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1.
Sepiapharaonis, the pharaoh cuttlefish was cultured through multiplegenerations in the laboratory (5 consecutive generations) using closed,recirculating water filtration systems. The eggs of the original parentalgeneration (GP) were spawned by a wild caught Gulf of Thailandfemale in alocal fisheries laboratory, then packed and shipped air cargo to Texas wherehatching occurred. The culture temperature ranged 25°–28°C, except for one generation that was chilled intentionallyto21 °C and then warmed to 25 °C after 9.6months. Spawning occurred as early as day 161. Spawning output was high in allgenerations except the group that was cultured at 21 °C. Eggfertility was low in captivity (< 20%), but hatchling survival was high(>70%). The average egg incubation time was 13.6 d at 25–28°C. The largest spawn resulted in 600 viable hatchlings andthesmallest resulted in 11 hatchlings. The cuttlefish ate a wide variety ofestuarine crustaceans and fishes as well as frozen shrimp. There were noapparent disease problems since survival from hatching to maturity was over70%.The average life span for cuttlefish cultured at 25–28°Cwas 8.9 months and 12.3 months at 21 °C. Size at hatching wasmeasured for fourth generation (G4) hatchlings; the mean weight athatching was 0.103 g and the mean mantle length was 6.4mm. The largest cuttlefish cultured was a male 300 mmML and 3,045 g; the oldest cuttlefish lived 340 d.This cuttlefish species presents an excellent choice for commercial mariculturebecause of its rapid growth, short life span, tolerance to crowding andhandling, resistance to disease and feeding habits.  相似文献   

2.
Feeding rates, growth rates and feed efficiency ratios were studied in experimentally reared juvenile cuttlefish Sepia officinalis which had been hatched from eggs collected from three different locations, Plymouth, North Wales and Southampton. Groups of newly hatched cuttlefish were either maintained at 19°C and well fed (experiment 1) or were maintained at ambient seawater temperature (7–16°C) with little food for 6 months so that their development was delayed and then transferred to optimum conditions (experiment 2). In the first investigation (expt 1), no significant differences in growth rates (3.72±0.08%, 3.75±0.04% and 3.55±0.04% body weight (BW) day?1 respectively), feeding rates (9.53±0.36%, 9.28±0.36% and 8.95±0.37% BW day?1 respectively) and feed efficiency ratios (38.11±1.67%, 40.52±1.78% and 39.96±1.78% respectively) were observed between cuttlefish from the 3 locations. During the second investigation (expt 2), cuttlefish, whose development was initially delayed after hatching and then were stimulated to grow under optimum conditions (19°C and fed), showed growth rates (3.46±0.08% BW day?1) similar to those held under optimum conditions of seawater temperature (19°C) and food supply shortly after hatching. Feeding rates and feed efficiency ratios were however significantly higher in cuttlefish maintained at 19°C compared to 11°C (8.27±0.14% BW day?1, 41.25±0.52% and 2.75±0.09% BW day?1, 24.87±1.87% respectively).  相似文献   

3.
Individual growth rates, feeding rates (%BWd?1) and food conversions for cuttlefish (S. officinalis) hatchlings and juveniles were determined during this study. A flow‐through system was used. Water temperature reached 30 °C during the hottest part of the day, gradually decreasing to 25 °C during the night; salinity varied between 37 ± 3 ppt and lights were kept on for 14 h day?1. Hatchlings were placed in separate compartments with a water volume of 1.2 L. Juvenile cuttlefish (from 0.5 to 25 g) were placed in bigger baskets, with a water volume of 5.2 L. Water flow was 120 L h?1. The biggest cuttlefish used in these experiments (> 25 g) were gathered in groups of five and placed in circular tanks (water volume of 250–300 L). Thus, results obtained in this case are means and not individual data. During the first 10, 20, 30 and 40 days, mean growth rates (of all individuals sampled by age group) decreased consistently (11.8 ± 4.1, 9.8 ± 1.8, 8.1 ± 2.2 and 7.3 ± 0.7%BW?1 respectively); in similar fashion, mean feeding rates decreased with age group (33.7 ± 13.5, 22.0 ± 7.9, 17.3 ± 3.9 and 16.7%BWd?1 respectively). Mean food conversions varied between 3.6 and 2.5 between the age groups. When grouping results by weight class, similar patterns occur, as growth and feeding rates decrease consistently as cuttlefish grow bigger. Highest mean growth and feeding rates are obtained by hatchlings (< 0.1 g) with 12.4 ± 4.5 and 35.3 ± 15.1%BWd?1, respectively, while the lowest growth and feeding rates were recorded for the largest animals, between 15 and 25 g (3.4 ± 1.1 and 10.8 ± 4.1%BWd?1 respectively). For these weight classes, mean food conversions varied between 2.7 ± 0.9 and 3.8 ± 2.8.  相似文献   

4.
Diapause eggs of Centropages hamatus were used to investigate the effect of temperature and duration of incubation on egg hatching. Eggs were incubated for 10, 12, 14, 16, 20, 24, 28, 32, 36 and 40 h at 15°C and 14L–10D. After incubation for the designated period, eggs were transferred to 25°C and monitored periodically to determine egg hatching. Control eggs were incubated solely at 15°C and monitored for egg hatching. The greatest daily hatching success of eggs occurred within 1 or 2 days after transfer from 15°C to 25°C, while the controls required 3–4 days. The cumulative hatching success of eggs was significantly lower than the control, with the exception of eggs held for at least 36 h at 15°C before transfer to 25°C. These results indicate that overall time to hatching of diapause eggs of C. hamatus can be reduced by transferring the eggs to a higher temperature, for example, 25°C, following a minimum period of time (36 h) at reduced temperature, for example, 15°C. Exposure to 15°C for only 10 h does not appear to be sufficient to result in any subsequent hatching at higher temperature.  相似文献   

5.
Two experiments, dealing with short‐term storage of ova and thermal conditions to optimize gamete and eggs management in hatcheries of the African catfish, Heterobranchus longifilis, were carried out. In the first experiment, ova collected by stripping from two strains of H. longifilis were stored for intervals up to 8 h at two temperature regimes: in a domestic refrigerator (3–5°C) and at ambient room temperature (20.5–22°C). In the second experiment, eggs were incubated from fertilization to hatching at different experimental temperatures (21, 25, 29, 32 and 35°C) to determine the effects of temperature on the kinetics of white egg appearance, hatching times and hatching quality. Gamete storage at warmer temperatures significantly prolonged viability irrespective of the strain used. In fact, the hatching rate for ova stored at 20.5–22 and 3–5°C for 5 h ranged between 75.2–79.3% and 6.5–9.4% respectively. Loss of viability was most noticeable after 6 h storage at ambient room temperature. Post‐storage viability significantly declined after 2 h exposure to the domestic refrigerator temperature. No hatching of normal larvae took place after 8 h post‐storage time. Results from the second experiment showed that time to maximum whitening of eggs was both strain‐ and temperature‐dependent. The time to maximum mortality of eggs was shorter in the Layo strain (LS) than in the Noun strain (NS), regardless of incubation temperature. The appearance of white eggs was shorter with increasing incubation temperatures. Hatching times decreased with increasing temperature, regardless of strain. Hatching took place from 21 to 27 h and 19 to 24 h after fertilization at temperature of 29°C, respectively, for NS and LS. The length of the hatching period was remarkably shorter for LS than NS at any tested incubation temperature, except 35°C. No hatching took place at 21°C. The highest proportion of normal larvae occurred at 25 and 29°C, respectively, for NS and LS. Hatching rate was highest at 25 and 29°C, respectively, for NS and LS. There was a significantly higher proportion of deformed larvae at 35°C regardless of the strain.  相似文献   

6.
This study tested two premises. First, that there is a range in water temperature within which to artificially incubate marron eggs and hatchlings, which enables a combination of rapid development, high survival, and the production of large juveniles. Second, that the population density of marron eggs and hatchlings incubated artificially can be increased without altering development time, survival, or juvenile body weight. Marron eggs were collected from 31 gravid females and artificially incubated at four water temperatures (i.e. 16°C, 20°C, 24°C, 28°C), while eggs were collected from eight females and artificially incubated at three population densities (i.e. 0.35, 0.70, 2.80 eggs mL?1). The eggs and hatchlings were assessed for development time and survival as they hatched and developed into juveniles. The juveniles were weighed at the conclusion of the incubation period. As water temperature was increased from 16°C to 28°C, there was an inverse curvilinear relationship between development time and water temperature, the time required for the eggs to hatch and develop into juveniles decreasing from 70.2 to 24.5 days. However, at 16°C and 28°C, the survival of the eggs and hatchlings was reduced (i.e. 83.8% and 87.7% at 20°C and 24°C vs. 70.4% and 57.5% at 16°C and 28°C), while incubation at 28°C resulted in the development of small juveniles (i.e. 29.7, 29.2, and 28.5 mg at 16°C, 20°C, and 24°C vs. 25.1 mg at 28°C). In comparison with water temperature, increasing the population density of the eggs and hatchlings from 0.35 to 2.80 eggs mL?1 did not have a significant effect on development time, survival, or juvenile body weight. The two premises tested in this study were supported, demonstrating that marron eggs and hatchlings can be artificially incubated at high population densities, and are most successfully incubated in water maintained between 20°C and 24°C.  相似文献   

7.
Loligo vulgaris is a commercially important squid throughout the Mediterranean region and is a candidate species in biomedical and aquaculture research. Some loligo species (L. opalescens, L. forbesi, Sepiteuthis lessoniana) have now been cultured through some successive generations in closed, recirculating seawater systems. The effects of salinity on hatching European Squid (L. vulgaris Lamarck, 1798) eggs were investigated during November 2004. The egg capsules were incubated directly in salinity of 32, 34, 36, 38, 40, 42 and 37 g L?1 (control group) at 19.8°C (SD 1.2°C), and a photoperiodicity of 12 h light:12 h dark for 16–23 days before hatching. In all treatments, the eggs were developed and hatched normally after 16–22 days at 32 g L?1, 17–22 days at 34, 18–21 days at 42 g L?1, 18–22 days at 36 and 40 g L?1, 19–22 days at 37 g L?1 and 19–23 h at 38 g L?1. In the experiments, the highest hatching rate and hatching success (HS) of the eggs were obtained at 38 g L?1 (hatching rate: 100% (SD 0%) and HS: 96.7% (SD 3.5%)) and the lowest hatching rate at 42 g L?1 (hatching rate: 3% (SD 6%) and HS: 0%). Dorsal mantle lengths (DML) of new hatchlings ranged from 2.08 to 2.80 mm. The present study showed that salinity affects the hatching rate and HS of eggs and first hatching time and DML of paralarvae in L. vulgaris. The squid eggs at stage 11 (I) can tolerate 5 g L?1 reduction and 3 g L?1 increase in salinity.  相似文献   

8.
Proteolytic activity of sea trout hatching liquid was examined towards casein and azocazein as a function of pH and temperature. The optimum pH for caseinolytic and azocaseinolytic activities were 9.4, and 9.0, respectively. At alkaline pH the enzyme was activated by low concentrations of Zn2+ ions (10−5 M). Maximum proteolytic activity of the hatching liquid was observed at 25°C. Temperatures exceeding 30°C caused a rapid reduction in enzyme activity. Proteolytic activity observed at 10°C was approximately 50% of that observed at 25°C. In general, a pseudo-Arrhenius plot indicated a Q10 of 1.6 between 6 and 25°C.  相似文献   

9.
Larvae and juveniles of the green abalone grow and survive well at temperatures in the range 20–28°C. A program of hatchery research with this species utilizing thermal effluent has been in progress since 1979 at the Redondo Beach Electric Power Facility, Southern California Edison Company. All life stages may be cultured in the effluent seawater with temperature regulation by admixture of ambient seawater when required. Using thermal effluent, growth rates of juveniles are increased by a factor of 1.5–2.0 over those reared at ambient temperatures (14–20°C) characteristic of southern California. The time to harvest for seed 1–2 cm is reduced from about one year to six months. While optimal temperatures for larvae and postlarvae fall in the range 20–24°C, juveniles are more broadly tolerant of heat and exhibit a growth optimum in the range 24–28°C. The upper lethal limit for juveniles is 31.5°C (LD50, 48 hours). Postlarvae, juveniles and adults have exhibited no indications of unfavorable conditions in power plant effluent. Young adults at 1.5 years (4–5 cm) became reproductively mature and yielded viable larvae. Both hatchery-reared and wild-caught adult green abalone were conditioned to spawning readiness during winter months (natural period, spring-fall) when held in a mixture of effluent and ambient seawater at 20–24°C. Progeny from this research were provided to the California Department of Fish and Game for release in experimental plants. Approximately 30,000 juveniles were produced in 1980–81.  相似文献   

10.
This study tested the effects of constant and varying temperatures on newly hatched yellowtail kingfish Seriola lalandi larvae in two experiments. In Experiment I, four constant temperatures (21, 23, 25, and 27 °C) were tested under fed or unfed conditions with the fish age from the day of hatch to 24 days post hatch (DPH). Temperatures at 25 and 27 °C reduced the time of fish to reach irreversible starvation, but did not affect the percentage of fish that were able to ingest food. Fish survivals at 21 and 23 °C were significantly higher than those at 25 °C by 24 DPH, but all fish died at 27 °C by 24 DPH in the treatment with food. In Experiment II, three constant temperatures (21, 23, and 25 °C) and two varying temperatures (21–23 and 21–25 °C) were compared using fish from hatch to 28 DPH. On 4 DPH, fish ingested more rotifers, but from 6 to 9 DPH, fish ingested fewer rotifers at 25 °C than at other temperatures. On 19 and 23 DPH, fish ingested more Artemia at 25 °C than at other temperatures. At 25 °C, fish selected for Artemia nauplii earlier than at other temperatures. Fish length and survival between constant temperatures (21 and 23 °C) were not significantly different, but fish survival at the constant 21 °C or at the 21–25 °C varying temperature was significantly higher than that at the constant 23 °C or at the 21–23 °C varying temperature. This study indicates that within the range of temperature tested, the optimal temperature for the first feeding larvae is 21–23 °C after hatch and mortality is likely to occur at ≥25 °C in the first 10 DPH, but fish grew faster at 25 °C after they adapted to the increasing temperature.  相似文献   

11.
Photosynthetic activities of seedlings of Zostera marina were successively measured using a gas volumeter for 6 days at seven light (0–400 μmol photons/m2 per s) and 11 water temperature conditions (5–35°C). The seedlings were collected from mature plants (Ise Bay, central Japan), and stored and cultured in incubators accurately controlled at each test temperature. The maximum gross photosynthesis (P maxg) was recorded at an optimal water temperature of 29°C after 0 days. After 6 days, P maxg appeared at 25°C and most plants cultured at 29–30°C bleached and withered after the drastic increase of light compensation point (I c). On the contrary, at 5–28°C, the photosynthetic activities either changed little (5–25°C) or recovered after a temporal reduction (26–28°C); seedlings survived and looked healthy after being cultured for 6 days. The recovery was thought to be an acclimation to tolerate higher water temperature. As a result, the critical upper water temperature for Z. marina seedlings was proposed as 28°C. The temperature was consistent with the previously reported maximum water temperature in habitats around the southern boundary of Z. marina in the northern hemisphere.  相似文献   

12.
In order to clarify the respiratory responses strategy of Amur sturgeon Acipenser schrenckii exposed to water temperature changes, respiratory parameters of the fish were studied under two temperature regimes: fish acclimated at 13°C for Group I, temperature was increased to 16°C, 19°C, 22°C and 25°C and then returned stepwise to 22°C, 19°C, 16°C and 13°C; and fish acclimated at 25°C for Group II, the water temperature was reduced in steps to 22°C, 19°C, 16°C and 13°C, subsequently, returned to 16°C, 19°C, 22°C and 25°C. The results showed that the respiratory frequency (fR), oxygen consumption rate (VO2) and gill ventilation (VG) of the fish were directly dependent on the acute temperature in both acclimation groups (p < .05). The initial 25°C VO2 in Group II was significantly higher than the initial 13°C VO2 in Group I (p < .05), but was significantly lower than that at 25°C in Group I (p < .05). In Group I, respiratory stroke volume (VS.R) of fish significantly increased or decreased with the acute temperature increases or decreases, respectively (p < .05); oxygen consumption efficiencies (EO2) of fish did not significantly show differences when temperature increased to 25°C from 13°C (p > .05), but the EO2 significantly declined while returning to acclimation temperature (p < .05). In Group II, the VS.R of the fish did not significantly change with acute temperature fluctuations between 25 and 13°C (p > .05), while the EO2 increased with acute temperature increases (p < .05). The Q10 values for fR, VO2, VS.R, VG and EO2 were 1.53–1.72, 1.92–2.06, 1.07–1.60, 1.78–2.44 and 1.11–1.65 at 13–25°C of temperature interval respectively. Amur sturgeon showed partial metabolic compensation to temperature changes. The study results suggest that the ability of Amur sturgeon to regulate metabolism in response to acute temperature changes makes this species good adaptability in the aquaculture rearing.  相似文献   

13.
High larval mortalities during rearing of gilthead bream, Sparus auratus L., led to experiments on the influence of salinity and temperature on eggs and yolk-sac larvae. Test salinities ranged from 5 to 70 ppt for eggs and from 15 to 45 ppt for larvae; experimental temperatures were 18–20°C for eggs and 18, 23 and 26°C for larvae. Spawning conditions were 18–20°C and 33–35 ppt salinity; the yolk-sac larvae were chosen from hatches obtained under similar conditions (18°C and 35 ppt salinity). For eggs the optimum survival range was found to be 30–50 ppt at 18°C and 15–60 ppt at 23°C, while that for yolk-sac larvae was 15–25 ppt at all three temperatures. Choosing normal development (no dorsal curvature) as the decisive criterion, the optimum salinity range for egg incubation was reduced to 30–40 ppt at 18°C and to 35–45 ppt at 23°C, while that for the yolk-sac stage remained 15–25 ppt at all test temperatures. Egg incubation was most successful at salinity-temperature combinations close to those during spawning, whereas salinity had to be reduced by at least 10 ppt for yolk-sac larvae.  相似文献   

14.
Abstract. First culture results are presented from four major experiments (lasting up to 478 days) on the commercially important squid species, Loligo forbesi Steenstrup, Details are provided on eggs, hatching, feeding, growth, survival, behaviour and sexual maturation. Best survival during the critical first 75 days was 15%. The hatchlings (up to 4.9mm mantle length, ML) are the largest among the genus Loligo , and the largest squid grown was a male 155mm ML and 124g. First schooling was observed only 40–50 days post-hatching. Spawning was not achieved although males reached maturity, females had maturing ova and mating was observed. The largest giant axon measured was 425μm in diameter (from a female 130mm ML), a size suitable for most biomedical applications. Laboratory data suggest a 2-year life cycle compared to fishery data which suggest a 1-year cycle.  相似文献   

15.
Senegal sole aquaculture is at present limited due to poor reproduction of captive breeders in many facilities. Temperature seems to play an important role in controlling reproduction of Solea senegalensis, and differences in temperature regimes followed by various hatcheries are likely to be responsible for lack of success in some of them. This work describes the reproduction of captive soles, held in facilities that used water at ambient temperature, from a marshy environment where this species naturally breeds. Acclimated sole breeders were kept for two consecutive years. The main spawning period occurred from February to May, with a secondary spawning in autumn. Total yearly fecundity ranged from 1.15×106 to 1.65×106 eggs kg−1 body weight. Of the total egg batches produced, only 5.4% corresponded to autumn spawns. The male population was found to produce sperm all year round, with a maximum proportion of 100% occurring in spring, and a minimum proportion of around 50% in summer. Females showed the more developed ovary stages from October to May, with partial regression in the summer months. During the main spawning period, eggs were produced between 46% and 69% of days.Spawning took place at temperatures from 13 to 23 °C, although higher fecundities (P<0.05) occurred between 15 and 21 °C. Within the range between 17 and 20 °C, the mean number of spawned eggs was 29,600±21,600 eggs day−1 kg−1. Most of the eggs (65–73%) were produced after temperature increased up to 2.5 °C within 3 days prior to spawning. Mean egg fertilization was 63.1±17% (year 2002) and 44.9±18% (year 2003), and hatching rates varied from 69.7±24% (2002) to 56.5 ±25% (2003). Weak correlations were found between either fertilization or hatching and fecundity, whereas a positive regression (P<0.05) indicated that higher hatching rates were achieved when fertilization increased. A weak, but significantly (P<0.05) positive correlation was found between egg fertilization and the spawning temperature. Present results indicate temperature is an important control factor for reproduction of S. senegalensis, and suggest it can be used to properly manage controlled captive reproduction of this species.  相似文献   

16.
This paper reports on a 4 × 4 factorial design experiment conducted to examine the combined effects of temperature and salinity on embryonic development and growth and survival of black-lip pearl oyster, Pinctada margaritifera (L.) larvae. The temperatures used were 20 °C, 25 °C, 30 °C and 35 °C, and the salinities were 25°/oo, 30°/oo, 35°/oo and 40°/oo. Response surface contour diagrams were generated from the survival and growth data to estimate optimal conditions. Normal development of embryos occurred only from 25 °C to 30 °C. The optimal conditions for maximum survival and growth were 26–29 °C and 28–32°/oo. Temperatures of 35 °C or greater were lethal for larvae and, at all temperatures tested, larval growth and survival were lowest at a salinity of 40°/oo.  相似文献   

17.
The purpose of this experiment was to observe the impact of stocking density on growth and food consumption of juvenile Sepia pharaonis reared at 23 and 28°C. Two groups of 32 cuttlefish each were reared in closed recirculating seawater systems with water temperatures of 23°C (group A) and 28°C (group B). Each group was divided into three treatments with two replicates per treatment: low-density (equivalent to 20 cuttlefish m−2), medium-density (equivalent to 100 cuttlefish m−2), and high-density (equivalent to 200 cuttlefish m−2). Measured amounts of live food were added three times a day and the wet body weight of each cuttlefish was measured once a week during the 42-day study. Cuttlefish in group B had higher growth rates and food consumption than cuttlefish in group A. The different stocking densities in group B affected the size of the cuttlefish whereas the stocking densities of the cuttlefish in group A treatments did not lead to different sizes between densities. Overall, the gross growth efficiency of the high-density treatments was lower than that of the low-density treatments, as was the weight of the cuttlefish in the high-density treatment. Although the wet weights of group A treatments were not significantly different (P > 0.05), the wet weights of the cuttlefish in the high-density, group B, treatment were lower than those in the low and medium density treatments. This decrease in individual size suggests that stocking densities of 100 to 200 cuttlefish m−2 may interfere with growth.  相似文献   

18.
The effects of animal density and water temperature on the culture of the mysid, Mysidopsis almyra (Bowman), in a static water system were evaluated. An initial set of experiments tested the effects of mysid density on production. Densities of 25, 37.5, 50, 100 and 200 mysids L–1 were placed in trays with 20 L of sea water. Temperatures were maintained at 26 ± 2 °C. A second set of experiments was conducted in the same system at three different temperatures (18 ± 1, 22 ± 1 and 26 ± 2 °C) using a mysid density of 50 mysids L–1 (1000 mysids tray–1). All experiments had a duration of 30 days. The mysids in all trials were cultured at 20 ± 2‰ salinity and fed Artemia nauplii enriched with marine fatty acids. There was a positive correlation between production and mysid densities up to populations of 100 mysids L–1; maximum production was 273 ± 99 hatchlings day–1. At a population density of 200 mysids L–1, high mortality and low production were recorded 4 days after the start of the experiment. The experiments testing different temperatures showed that mysid production was higher at 22 ± 1 °C, although this result was not significant (P > 0.05). Growth rates and hatchling survival after 7 days were significantly higher (P < 0.05) at 26 ± 2 °C compared to survival and growth at 18 or 22 °C.  相似文献   

19.
Constant and oscillating egg incubation temperatures on embryonic development and early larval morphology were studied in longfin yellowtail (Seriola rivoliana Valenciennes). We investigated the effects of constant temperatures from 16 to 32°C on embryo development and larval morphology at hatch, and whether oscillating temperature during embryogenesis could lead to larval morphological variations. After hatching, larval morphology and development during yolk sac (YS) utilization were examined in larvae at constant temperatures and larvae at 25°C that had oscillating temperature during egg incubation. Hatching rates were > 75%, only decreasing to ~ 50% at 30°C. At constant temperatures, the largest larvae occurred at 22 and 24°C. The oscillating temperature did not affect the timing of embryo development but resulted in larger and smaller larvae with a smaller and bigger YS, respectively, with a similar hatching time. Therefore, a growth response occurred in embryos during a window of development before hatching, depending on the adaptive response to temperature (spawn‐specific). After hatching, most of the YS was absorbed within 24 hr in all treatments, and the growth of the larval head was a priority with an optimal development at 26°C. There was compensatory growth in smaller larvae resulting in similar sizes after YS utilization, but larvae showed variations in body structure that could be important in further aquaculture research.  相似文献   

20.
The high mortality rate of reared Japanese eel Anguilla japonica larvae is largely due to lower growth rate and the higher rate of deformed larvae. To establish an effective rearing protocol for this species, we examined the effects of water temperature and feeding regimes on their growth and notochord kyphosis. Larvae at 165 days post hatching were reared for 28 days at mean temperatures of 24, 25 and 27 °C, and were fed 4 or 6 times per day. Larval growth rate was significantly higher in larvae reared at 24–25 °C and fed 6 times per day. However, growth rate was significantly reduced at 27 °C, suggesting a shortage of metabolic energy due to an elevated cost of the higher basal metabolic rate at higher temperatures and low nutritional performance of currently used artificial diet. Notochord kyphosis was promoted by elevated water temperature, and two-way ANOVA showed that water temperature and feeding frequency had combined effects on the deformity. These findings suggest the importance of concurrently manipulating both environmental and nutritional factors to produce healthy eel larvae in captivity.  相似文献   

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