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1.
吡喹酮防治犬细粒棘球绦虫效果观察胡义,保广祺,郭红伟,周翰信(青海省海北州牧科所)为检验、考核对犬细粒棘球绦虫(E.gro-nulosus)综合防治效果,于1991年11月至1994年11月在海晏县全境内进行了细粒棘球线虫和棘球蚴病综合防治效果观察。...  相似文献   

2.
青海省不同地区棘球蚴感染率的差异较大,从1989—2003年的资料分析,都兰县羊棘球蚴感染率98.53%,门源县羊棘球蚴平均感染率为29.42%,达日县牦牛的的感染率86.80%,某军牧场4岁以上牛感染率100%。天峻县牦牛棘球蚴感染率为37.8%,玉树州犬细粒棘球绦虫感染率78.13%,海南州犬细粒棘球绦虫感染率为19.30%。青海人群本病的平均患病率为2%,少数牧区高达7%~10%。海北州从1991—2003年间开展棘球蚴病综合防治,牛棘球蚴平均感染率降至5.23%。羊棘球蚴平均感染率降至8.50%。犬细粒棘球蚴绦虫感染率由综防前的63.64%下降为零,防治成效显著。  相似文献   

3.
1989年某军牧5志向匀以下羊棘球蚴的平均感染率为80%,牛为66.67%;彩用氢溴酸槟榔碱驱虫法监测犬细粒棘球绦虫的感染率为38.46%;1969年以来,人间包虫病累计手术病例数超过当地居民数的0.5‰。1990-1994年施行控制养犬数量,吡喹酮定期给犬驱虫、妥盖处理患病脏器等综合性防治,连续防治5年后监测结果:5岁以下羊棘球是细的平均感染率下降至9.58%,牛棘球蚴的感染率下降至6.06%,犬细粒棘球绦虫的感染率下降为零。在人间再未发现新的包虫病病例。  相似文献   

4.
棘球蚴病综合防治技术推广应用   总被引:6,自引:6,他引:0  
1996 —1998 年在海北州3 县5 场进行棘球蚴病综合防治技术推广,对项目区21 万只犬应用吡喹酮药物采取“犬犬投药、季节驱虫”的模式,连续三年累计驱虫达3483 万只( 次) ,平均驱虫密度达95 % ,犬细粒棘球绦虫感染率由综防前的6207 % 下降到零;1 ~3 岁幼年羊棘球蚴感染率由综防前的3631 % 、4006 % 和5786 % 分别下降到780 % 、1016 % 、1343 % ;幼年羊多头蚴感染率由综防前的429 % 下降到032 % ;1 ~3 岁牛棘球蚴由综防前的1940 % 、4261 % 和5926 % 分别下降到零和243 % 、566 % ,效果明显、经济效益显著。  相似文献   

5.
贵南县包虫(棘球蚴)病基线调查   总被引:1,自引:1,他引:0  
为了全面掌握贵南地区包虫(棘球蚴)病流行程度,制定防治对策,2004年5~10月对本地区人、犬、牛、羊的包虫病感染情况作了全面调查。结果表明:人包虫病患病率0.79‰,犬细粒棘球绦虫平均感染率为28.33%,牛棘球蚴平均感染率为58%,羊棘球蚴平均感染率为43.73%,表明贵南县为棘球蚴病高发区,应采取综合性防制措施,有效控制包虫病的发生。  相似文献   

6.
通过对玛曲县欧拉、尼玛等乡牧场棘球蚴病高发区牛15头、羊100只、犬15条剖检和分析发现,棘球蚴感染率牦牛为66.67%、羊为50%、母羊为75%,母犬细粒棘球绦虫的感染率为27%、人的棘球蚴感染率为0.3%。对1~5岁绵羊各50只,进行的间接血凝感染抗体检测表明:1岁羊血清滴度较低,2岁和4岁羊的滴度较高,而3岁和5岁羊的滴度略低于前一年龄组。酶联免疫吸附试验结果:1岁羊血清平均值最低,2岁最高,以后各年龄组略微降低,并大致保持平稳状态。  相似文献   

7.
经调查海晏地区绵羊棘球蚴病的感染率为46.13%,牦牛棘球蚴病的感染率为16.85%,犬细粒棘球绦虫感染率为33.33%,人的棘球蚴患病率为0.69%。这与1991年的调查数相比感染率有所下降,而与综防后的1994年相比,感染率大幅度回升,说明海晏地区棘球蚴病的危害依然严重,需在今后的工作中继续加强防治,促使棘球蚴病达到稳定控制。  相似文献   

8.
青海高原地区棘球绦虫/棘球蚴病的流行概况   总被引:3,自引:2,他引:1  
对青海高原地区犬棘球绦虫感染率和人、动物棘球拗的感染情况资料进行整理和分析。结果:犬的棘球绦虫感染率为19.3%-82.3%;家畜棘球蚴感染率在80.0%以上者占全省40个县、市的52.5%;人体因包虫病住院接受手术治疗者占人群均数的0.2‰-1‰,感染率平均为1%-5%。结果表明该地区为棘球蚴病的高度流行区,并就防治提出了建议。  相似文献   

9.
为了解青海省家畜棘球蚴病流行现状,给防治工作提供科学依据,2011年,在不同自然环境条件下选择5个县(市)对牛、羊棘球蚴病进行了流行病学调查.结果表明,5县牛、羊棘球蚴平均感染率和感染强度分别为27.09%、33.89%和0.5、1.3,犬细粒棘球绦虫平均阳性率为10.91%.  相似文献   

10.
2000年5月-12月在果洛甘德县进行了家畜棘球蚴病感染情况调查。共检查羊1700只,阳性818只,感染率为48.12%;牛96头,阳性54头,感染率56.25%;犬细粒棘球绦虫感染调查犬63只,阳性22只,感染率34.92%;对人包虫的感染情况进行了回顾性调查表明,甘德地区属棘球蚴病高发流行区。  相似文献   

11.
During routine necropsy of a 28-year-old horse with intestinal volvulus, numerous hydatid cysts were discovered in the liver. Viable protoscolices of Echinococcus granulosus were obtained from the cyst. As a 4-year-old, this horse had been imported from an area that is enzootic for equine hydatidosis.  相似文献   

12.
The prevalence of cysticercosis and hydatidosis in Ethiopian sheep   总被引:1,自引:0,他引:1  
Five hundred and sixty sheep, slaughtered at an Addis Ababa abattoir, were examined for cysticercosis and hydatidosis. The prevalence of the metacestodes of Taenia hydatigena was 37.1% and that of Echinococcus granulosus 16.4%. In addition, the age and sex prevalences of hydatid cysts, their organ distribution and the ratio of fertile cysts were determined.  相似文献   

13.
为建立一种快速、高效的诊断家畜细粒棘球蚴病方法,提取绵羊细粒棘球蚴包囊新鲜囊液,盐析囊液抗原,点样于硝酸纤维膜,以胶体金-驴抗羊IgG和胶体金-兔抗鼠IgG为检测标记物,采用垂直流渗滤装置检测绵羊与人工感染细粒棘球蚴小鼠血清和全血特异性抗体。患病绵羊阳性血清及全血检出率在90.91%~94.4%,细粒棘球蚴感染小鼠血清及全血检出率均为100%;细粒棘球蚴阴性羊血清和全血假阳性率为4.00%~4.59%;与脑多头蚴病血清交叉反应率为28.57%(2/7)。研究结果表明细粒棘球蚴全血金标渗滤法(DIGFA)可应用于绵羊棘球蚴病的诊断与检疫。  相似文献   

14.
Sentinel lambs were used to identify young Echinococcus granulosus infections in sheep, to provide an early indication of the progress of the South Powys Hydatidosis Control Scheme. Four sentinel lambs were purchased on each of 60 farms, from inside and outside the control area; they were examined when approximately six, 10 and 15 months of age. Gross examination, thin slicing of organs and histological examination of the lesions in the viscera revealed no E granulosus hydatid cysts in lambs born within the control area, whereas 25 per cent of the 15-month-old lambs from outside the area harboured E granulosus cysts (less than 1 to 2 mm in diameter). Lambs from E granulosus infected farms had significantly higher anti-E granulosus ELISA antibody titres than lambs from uninfected farms. It was concluded that within one year of beginning to treat dogs with praziquantel every six weeks the transmission of E granulosus to sheep had ceased. In contrast, this treatment did not prevent infections with Taenia hydatigena or T ovis; an examination of the 240 lambs revealed T hydatigena in 33.3 per cent of them, Tovis in 4.2 per cent, Dictyocaulus filaria in 12.1 per cent and Meullerius capillaris in 49.2 per cent.  相似文献   

15.
In the current study, soluble proteins prepared from 200 mature Echinococcus granulosus and protoscolices of sheep hydatid cysts were applied to immunize sheep and mice respectively. The samples were mechanically homogenized in a blender, sonicated and the final yield was maintained at -20 degrees C until analysis. Hydatid fluid was isolated from liver or lung of sheep under sterile conditions. In the first experiment, 15 mice were randomly allocated to three groups of five mice each. Each mouse in groups 1 and 2 was immunized with 100 microg of hydatid fluid and protoscolex proteins in 100 microl of phosphate-buffered saline (PBS) and emulsified with an equal volume of Freund's complete adjuvant (FCA) respectively. The mice of group 3 were immunized with adjuvant in PBS. The mice were boosted 4 weeks after the first vaccination with the same preparation except that FCA was replaced by Freund's incomplete adjuvant (FIA). In the second experiment, eight male or female lambs 4-6 months of age, were allocated to two groups of four lambs each. Each lamb in the test group was vaccinated subcutaneously in the neck with a 2-ml dose of vaccine (1 mg of whole body protein of E. granulosus dissolved in 1 ml of PBS plus 1 ml of FCA). Control lambs were vaccinated with adjuvant in PBS. Lambs were boosted the same way as in the first experiment. Three weeks after the second vaccination, each mouse and lamb received a challenge infection with 2000 protoscolices intraperitoneally and each lamb additionally received 10 gravid E. granulosus. All mice and sheep were killed after 7 months and examined for hydatid cysts. In these studies, protective immunity was induced in mice with protoscolex protein and with hydatid fluid, and in sheep with whole-body homogenate of E. granulosus and the levels of protection afforded were found to be 72.1, 82.6 and 90.9% respectively.  相似文献   

16.
The aim of this study was to develop an immunological method for the identification of sheep infected with Echinococcus granulosus which would allow the monitoring of animals imported into countries free from hydatidosis and as an aid to countries where control schemes for the disease are in operation. Three enzyme-linked immunosorbent assays (ELISAs) were developed and validated, using as antigen either a purified 8 kDa hydatid cyst fluid protein (8kDaELISA), a recombinant EG95 oncosphere protein (OncELISA) or a crude protoscolex preparation (ProtELISA). Sera used for the assay validations were obtained from 249 sheep infected either naturally or experimentally with E. granulosus and from 1012 non-infected sheep. The highest diagnostic sensitivity was obtained using the ProtELISA at 62.7 and 51.4%, depending on the cut-off. Assay sensitivities were lower for the 8kDaELISA and the OncELISA. Diagnostic specificities were high, ranging from 95.8 to 99.5%, depending on the ELISA type and cut-off level chosen. A few sera from 39 sheep infected with T. hydatigena and from 19 sheep infected with T. ovis were recorded as positive. Western immunoblot analysis revealed that the dominant antigenic components in the crude protoscolex antigen preparation were macromolecules of about 70-150 kDa, most likely representing polysaccharides. This study demonstrated that the ProtELISA was the most effective immunological method of those assessed for detection of infection with E. granulosus in sheep. Because of its limited diagnostic sensitivity of about 50-60%, it should be useful for the detection of the presence of infected sheep on a flock basis and cannot be used for reliable identification of individual animals infected with E. granulosus.  相似文献   

17.
This study was conducted in order to determine the prevalence and fertility of hydatid cysts in sheep in Kirikkale, Turkey. A total of 3.2% of 553 lambs and 50.9% of 1320 adults were found to be infected with hydatid cysts. The most common locations of cysts were in the liver and lung. Liver cysts tended to be more fertile than lung cysts (81.53% v. 76.47%), though the difference was not significant. The mean number of viable protoscoleces in the liver and lung cysts were 12,400 and 5,800, respectively. In general, 1-10 cysts were found in liver (51.8%) and lung (64.7%). The findings of this study indicate that hydatid cysts are common in sheep and the fertility rate of cysts is quite high. Sheep play an important role in the life cycle of Echinococcus granulosus in this region.  相似文献   

18.
In the present study, 115 stray dogs (56 males and 59 females, mixed breed), 86 golden jackal (Canis aureus, 42 males and 44 females), 60 red foxes (Vulpes vulpes, 33 males and 27 females), and three female wolves (Canis lupus) were examined for Echinococcus granulosus infection, as well as, 32,898 sheep, 10,691 goats, 15,779 cattle and 659 buffaloes for hydatid infection from five provinces in western Iran during 3 years (1997-2000). Meanwhile fertility rates of different types and forms of cysts isolated from infected animals and the viability of protoscolices were also determined. Results indicated that 19.1% of the dogs, 2.3% of the golden jackals and 5% of the red foxes were infected with Echinococcus granulosus. 11.1% of the sheep, 6.3% of the goats, 16.4% of the cattle and 12.4% of the buffaloes were also found to be infected with hydatid cyst. The cysts isolated from liver and lungs of the sheep show higher fertility rate than the cysts of liver and lungs of goats, cattle and buffaloes.  相似文献   

19.
A 14-year-old male Chapman's zebra had been treated for a cervical granulomatous cellulitis for 3 months prior to sudden death associated with myocardial fibrosis. Incidental findings at necropsy included multiple 3-8-cm unilocular cysts in the liver and lungs. Cysts contained either a clear fluid with or without hydatid sand or a gelatinous substance with foci of mineralization. Light and scanning electron microscopic examination of the hydatid sand revealed free protoscolices as well as intact and ruptured brood capsules with protoscolices attached to the germinal membrane. The protoscolices had 2 rows of 36-38 rostellar hooks with a length of 25-30 microm. The cyst wall consisted of inner germinal, intermediate laminated, and outer adventitial layers. Hydatidosis caused by Echinococcus granulosus was diagnosed based on the unilocular cysts, multiple protoscolices formed in a brood capsule, typical trilayered cyst wall, and herbivorous intermediate host. This is the first reported case of animal hydatidosis in Taiwan. The infection is thought to have been established 12 years ago in South Africa prior to importation.  相似文献   

20.
The aim of this study was to determine the fertility and viability of hydatid cysts in slaughtered sheep and cattle. Cysts were collected from the liver and lungs of 169 sheep and 171 cattle infected with Echinococcus granulosus when slaughtered in industrial abattoir in Sari, Iran, 2007–2008. Fertility was determined by the examination of cyst fluid for the presence of protoscoleces. The viability of the protoscoleces was assessed by staining with 0.1% aqueous eosin solution. The fertility rates of hepatic cyst of sheep and cattle were 47.1% and 1.4%, respectively and the fertility rates of pulmonary cyst of sheep and cattle were 39.4% and 8.1%. In the sheep, the fertility of cysts in the liver was higher than that in lungs, but in the cattle the fertility of cysts in lungs was higher than liver. The viability of protoscoleces of fertile cysts for sheep and cattle were about 76.9% and 82.5%, respectively. Based on the finding in the present study, effort should be made to control transmission of cystic echinococcosis by safe disposal of Echinococcus cysts such that dogs cannot have access to the cysts.  相似文献   

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