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1.
病毒病是马铃薯生产中的主要病害之一,侵染马铃薯的病毒中,影响较为严重和发生较为普遍是卷叶病毒(potato leaf roll virus,PLRV)引起的卷叶病。本研究通过田间性状调查得到50株马铃薯‘合作88’自交F2代抗PLRV性状分离群体,首先利用DAS-ELISA明确侵染病毒的类型,随机选取5株抗病群体和5株感病群体,通过Illumina 2X覆盖度重测序,随后以马铃薯双单倍体DM为参考基因组进行单倍体组装和注释。通过基于Perl语言下的MISA程序进行全基因组SSR挖掘和比对,共筛选出277个与抗卷叶病相关的特异性SSR标记;接下来随机选取180个SSR标记并设计引物,PCR扩增后通过LabChip GX Touch检测得到232个等位位点,其中多态性位点152个,基于Minimum-Evolution的聚类分析发现,在遗传相似性系数为0.80时,能把抗PLRV两个性状分离群体区分。利用JoinMap 4.0构建了‘合作88’抗PLRV连锁的遗传图谱;遗传图谱共包含8个连锁群,总长度为2684.3 cM,标记间平均距离11.57 cM。最后用TetraploidMap检测到5个与抗卷叶病相关的QTL,其遗传贡献率分别为4.31%、8.70%、10.89%、13.52%、7.82%。基于mapping的单倍体测序数据,5个QTL分别被定位于第Ⅹ号、Ⅲ号、Ⅰ号、Ⅷ号、Ⅸ号染色体。本研究可为高通量开发马铃薯抗卷叶病分子标记提供技术参考,也可为精细定位马铃薯优良性状相关基因提供理论依据。 相似文献
2.
西藏三联小穗小麦中三联小穗性状控制基因的SSR分子标记定位 总被引：1，自引：1，他引：0
西藏三联小穗小麦是中国西藏地区一种独特的小麦地方品种,拥有特殊的三联小穗性状,超多的小穗数和小花数。分子定位控制三联小穗基因的基因座,发掘与之紧密连锁的分子标记,可为小麦高产育种提供分子标记辅助选择工具。本研究利用西藏三联小穗小麦的衍生系TTSW-5与普通穗型小麦,间3和川麦55,分别构建F2群体,成熟后进行穗部性状的表型分析和SSR基因型鉴定。性状表型遗传分析表明,西藏三联小穗小麦的三联小穗性状由两个独立遗传的隐性基因控制;通过SSR标记鉴定来自TTSW-5/间3组合的F2群体,在2A染色体上检测到1个与三联小穗性状相关的QTL,定位于SSR标记Xgwm275和Xgwm122之间,两标记间的遗传距离为6.6cM,该QTL的LOD值为6.19,可解释的表型变异值为33.1%,初步命名为qTS2A-1。我们推测qTS2A-1可能是控制三联小穗性状相关的主效QTL,SSR标记Xgwm275和Xgwm122可能可用于三联小穗性状的辅助选择。 相似文献
3.
杂交稻部分不育系与恢复系的SSR分类 总被引：20，自引：3，他引：20
选用分布于水稻(Oryza sativa L.)12条染色体上的36对SSR(simple sequence repeats)引物，分析了5个光温敏核不育系、7个细胞质雄性不育系及54份来自不同生态类型恢复系或品种的遗传差异。在供试材料中共检测出300条多态性片段，平均每对SSR引物检测到8.33条。分析结果表明，(1) SSR标记明确地把供试的66份水稻材料中的65份区分开来，和已知系谱的亲缘关系多数吻合，与表型性状聚类结果也有一定的相似性。(2) 以GD=0.73为标准，可准确地将籼稻、粳稻、野生稻三大类区分开；以GD=0.63为标准，又可区分出籼稻大类中带有粳稻或野生稻血缘的品种。表明SSR标记对籼稻、粳稻、野生稻品种的分类灵敏度高、结果可靠；并筛选出可用于鉴别水稻籼粳类型的10对特异性引物。(3) 以GD=0.56为标准，将供试材料划分在8个生态类型中，为生产上杂优组合亲本的选配提供了一些有益的参考。同时，生产上常用的一些杂交稻亲本划分在不同的生态类型中，表明强优势组合亲本间的遗传差异一般较大。 相似文献
4.
Quantitative trait loci mapping of freezing tolerance and photosynthetic acclimation to cold in winter two‐ and six‐rowed barley 
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下载免费PDF全文 Photoacclimation (PA) and freezing tolerance (FT) have been identified as closely related traits, due to common mechanisms of environmental control. In this study, diversity array technology (DArT) was used for identification of the quantitative trait loci (QTL) of FT and PA in winter barley. Simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers were subsequently used to saturate QTL regions. Two F2 mapping populations were created, for two‐rowed (P44) and six‐rowed barley (CaP). Different regions of the genome were responsible for differences in traits between parents in these two populations. Eleven QTLs were identified in the P44 population, including five typical for FT and PA, on chromosomes 2H, 3H and 7H. In the CaP population, only one QTL connected with PA and 10 connected with FT were found on all chromosomes except 2H. Our results demonstrate that different sets of markers should be applied in marker‐assisted selection for FT in two‐ and six‐rowed barley, as several loci determine FT at the level of biparental crosses. 相似文献
5.
Abbas Rezaeizad Benjamin Wittkop Rod Snowdon Maen Hasan Valiollah Mohammadi Abbas Zali Wolfgang Friedt 《Euphytica》2011,177(3):335-342
Association mapping identifies quantitative trait loci (QTLs) by examining the marker-trait associations that can be attributed
to the strength of linkage disequilibrium between markers and functional polymorphisms across a set of diverse germplasm.
In this study, association mapping was performed to detect QTL-linked and genome wide SSR markers linked to phenolic compounds
of extraction meal in a population of 49 genetically diverse oilseed rape cultivars of dark-seeded, winter-type oilseed rape
accessions. Correction for population structure was performed using 559 genome wide SSR markers. Results showed that seed
colour is an important contributor to seed meal quality. Totally, 52 SSR markers linked to phenolic compounds were detected,
five of them being QTL linked markers. Some of these markers were already mapped on Brassica napus chromosomes that contain known QTL controlling oilseed rape meal quality traits. Our results demonstrate that association
mapping is a useful approach to complement and enhance previous QTL information for marker-assisted selection. 相似文献
6.
Comparisons of RFLP and PCR-based markers to detect polymorphism between wheat cultivars 总被引：1，自引：0，他引：1
Previously chromosome 3A of wheat (Triticum aestivum L.) was reported to carry genes influencing yield, yield components, plant height, and anthesis date. The objective of current
study was to survey various molecular marker systems for their ability to detect polymorphism between wheat cultivars Cheyenne(CNN)
and Wichita (WI), particularly for chromosome3A. Seventy-seven `sequence tagged site' (STS), 10simple sequence repeat (SSR),
40 randomly amplified polymorphic DNA (RAPD) markers, and 52 restriction fragment length polymorphism (RFLP) probes for wheat
homoeologous group 3 chromosomes, were investigated. Three (3.9%) STS-PCR primer sets amplified polymorphic fragments for
the two cultivars, of which one was polymorphic for chromosome 3A. Sixty percent of SSR markers detected polymorphism between
CNN and WI of which 50% were polymorphic for chromosome 3A. Twenty percent of RAPD markers detected polymorphism between CNN
and WI in general, but none of these detected polymorphism for chromosome 3A. Of the fifty-two RFLP probes, 78.8% detected
polymorphism between CNN and WI for group 3 chromosomes with one or more of seven restriction enzymes and 42% of the polymorphic
fragements were for chromosome 3A. These high levels of RFLP and SSR polymorphisms between two related wheat cultivars could
be used to map and tag genes influencing important agronomic traits. It may also be important to reconsider RFLP as the most
suitable marker system at least for anchor maps of closely related wheat cultivars.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
7.
陆地棉亚红株突变体基因的初步定位 总被引：6，自引：0，他引：6
陆地棉亚红株突变体PD-17与GK19杂交F1代表现出良好的光合作用效率,具有较高的超亲优势,是潜在的优良种质资源。寻找与该突变基因Rs连锁的分子标记并进行染色体定位,对于该基因的进一步精细定位或克隆具有重要的意义。以亚红株突变体PD-17与GK19配置的BC1群体为作图群体,选用覆盖棉花所有已鉴定染色体及大多数连锁群的419对SSR引物,利用BSA（bulked segregation analysis）法筛选有多态性差异引物,然后根据群体单株基因型进行作图分析。结果显示,位于第7条染色体上的5个分子标记与Rs基因相连锁,SSR标记BNL2634与Rs基因的遗传距离较小,约为10.3 cM,SSR标记CIR393位于Rs基因另一侧,与Rs基因的遗传距离约为29.1 cM,由此,可将Rs基因定位于第7染色体上。 相似文献
8.
Muhammad N. Sattar Muhammad Javed Syed B. Hussain Muhammad Babar Peng W. Chee Zafar Iqbal Muhammad Munir Sallah A. Al-Hashedi 《Plant Breeding》2023,142(2):247-257
Cotton leaf curl disease (CLCuD) is a major threat to cotton production in Asia and Africa. Using marker-assisted breeding can be the best sustainable approach to tackle CLCuD. Identification of new QTLs in the indigenous cotton germplasm is necessary to combat CLCuD. The current study was designed to construct a genetic linkage map of bi-parental F2:F3 populations developed from highly tolerant MNH-886 and highly susceptible S-12 cotton cultivars. One hundred seven CLCuD-associated simple sequence repeat (SSR) marker alleles were identified as polymorphic and three new QTLs were found on chromosomes C11, C19 and C21. Two QTLs on chromosomes C11 and C19 were detected in both F2 and F3 populations in the region flanked by SSR markers CIR316 and BNL4094, and BNL285 and BNL3348, respectively. Whereas, one QTL on chromosome C21 was detected in the region flanked by SSR markers JESPR158 and JESPR135 in both F2 and F3 generations. The CLCuD-associated QTLs identified in this study can help fine-tune the molecular mapping of the QTLs on the cotton genome against CLCuD. 相似文献
9.
The Rf1 gene in sunflower can effectively restore the pollen fertility of PET1 cytoplasm in male-sterile lines and has been widely used in commercial hybrid production. Identifying molecular markers tightly linked to this gene will be useful in marker-assisted selection to develop maintainer and restorer lines. Rf1 has been mapped to Linkage Group (LG) 13 of the public sunflower simple sequence repeat (SSR) map by aligning maps constructed from different populations and only one SSR marker was reported to be loosely linked to Rf1 . This paper reports the result of applying target region amplification polymorphism (TRAP) and SSR markers to map and develop a sequence-tagged site (STS) marker tightly linked to Rf1 using two populations derived from a cross between two U.S. public sunflower lines, RHA439 and cmsHA441. An SSR marker, ORS511, was 3.7 cM from the Rf1 gene and a TRAP marker, K11F05Sa12-160, was linked to Rf1 at a distance of 0.4 cM. This TRAP marker was converted to an STS marker for using in sunflower breeding. 相似文献
10.
Soybean (Glycine max [L.] Merr.) is cultivated primarily for its protein and oil in the seed. In addition, soybean seeds contain nutraceutical compounds such as tocopherols (vitamin E), which are powerful antioxidants with health benefits. The objective of this study was to identify molecular markers linked to quantitative trait loci (QTL) that affect accumulation of soybean seed tocopherols. A recombinant inbred line (RIL) population derived from the cross ‘OAC Bayfield’ × ‘OAC Shire’ was grown in three locations over 2 years. A total of 151 SSR markers were polymorphic of which a one‐way analysis of variance identified 42 markers whereas composite interval mapping identified 26 markers linked to tocopherol QTL across 17 chromosomes. Individual QTL explained from 7% to 42% of the total phenotypic variation. Significant two‐locus epistatic interactions were identified for a total of 122 combinations in 2009 and 152 in 2010. The multiple‐locus models explained 18.4–72.2% of the total phenotypic variation. The reported QTL may be used in marker‐assisted selection (MAS) to develop high tocopherol soybean cultivars. 相似文献
11.
Simple sequence repeat markers linked to a major QTL controlling pod shattering in soybean 总被引：3，自引：0，他引：3
H. Funatsuki M. Ishimoto H. Tsuji K. Kawaguchi M. Hajika K. Fujino 《Plant Breeding》2006,125(2):195-197
Shattering of soybean pods prior to harvest leads to a reduction in yield. In order to identify simple sequence repeat (SSR) markers linked to quantitative trait loci (QTLs) conditioning pod shattering, QTL analysis was conducted using an recombinant inbred line (RIL) population segregating for this trait. The degrees of pod‐shattering resistance were evaluated by heat treatment applied to pods harvested from plants in the field and in a growth chamber. Composite interval mapping identified one major QTL between SSR markers Sat_093 and Sat_366 on linkage group J for both environments. The position and the effect of this QTL were confirmed in an F2 population derived from a cross between the pod shattering‐susceptible parental cultivar and a pod shattering‐resistant RIL. The SSR markers linked to the major QTL will be useful for marker‐assisted selection in soybean‐breeding programmes. 相似文献
12.
以禾谷镰刀菌(Fusarium graminearum Schwabe)菌株进行穗部喷雾和单花滴注接种,评价了10个小麦抗源的赤霉病和脱氧雪腐镰刀菌烯醇(DON)积累抗性。结果表明,望水白、苏麦3号、延岗坊主、繁60096属于高抗品种,Frontana表现感病,其余品种表现中抗。除Frontata外,所有抗源DON含量在3 mg/kg以下。不同接种方法间、不同致病菌株间的病小穗率和DON含量以及同一处理内的病小穗率和DON含量间呈极显著相关。利用与已报道的赤霉病抗性QTL相关SSR引物对供试材料进行PCR扩增,比较扩增产物等位位点的差异,除4B染色体的GWM113标记外,其余标记在品种间具有2~8个等位位点,多态信息含量为0.14~0.85。单倍型分析表明,延岗坊主具有与望水白一致的3B主效QTL的SSR标记位点,扬麦158和新中长分别在2D和4B上具有多个与武汉1号一致的抗性QTL相关SSR位点,翻山小麦在3B和6B上具有多个与苏麦3号或望水白一致的抗性QTL相关SSR位点,繁60096在2D上有多个与武汉1号一致的QTL相关SSR标记,而镇麦7459和温州红和尚与已报道的小麦赤霉病抗性多数SSR位点不一致,可能具有不同的抗性基因。 相似文献
13.
14.
Two genic male sterile (GMS) lines, Lang-A conditioned by ms 15 and Zhongkang-A conditioned by ms 5 ms 6 duplicate recessive genes in Gossypium hirsutum L., were chosen to map GMS genes. These two lines were crossed with Gossypium barbadense cv. 'Hai7124' to produce segregating populations. The ms 15 gene was mapped on chromosome 12, and was flanked by two simple sequence repeat (SSR) markers, NAU2176 and NAU1278, with a genetic distance of 0.8 and 1.9 cM respectively. The ms 5 and ms 6 genes were mapped to one pair of homoeologous chromosomes, ms 5 on chromosome 12 flanked by three SSR markers, NAU3561, NAU2176 and NAU2096, with genetic distances of 1.4, 1.8 and 1.8 cM, respectively, and ms 6 on chromosome 26 flanked by two SSR markers, BNL1227 and NAU460, with a genetic distance of 1.4 and 1.7 cM respectively. These tightly linked markers with the ms 15 , ms 5 and ms 6 genes can be used in the marker-assisted selection among segregating populations in a breeding programme, and provide the foundation for gene isolation by map-based cloning for these three genes. 相似文献
15.
Validation of a major QTL for scab resistance with SSR markers and use of marker-assisted selection in wheat 总被引：10，自引：1，他引：10
The objectives of this study were to validate the major quantitative trait locus (QTL) for scab resistance on the short arm of chromosome 3B in bread wheat and to isolate near‐isogenic lines for this QTL using marker‐assisted selection (MAS). Two resistant by susceptible populations, both using ‘Ning7840’ as the source of resistance, were developed to examine the effect of the 3BS QTL in different genetic backgrounds. Data for scab resistance and simple sequence repeat (SSR) markers linked to the resistance QTL were analyzed in the F2:3 lines of one population and in the F3:4 lines of the other. Markers linked to the major QTL on chromosome 3BS in the original mapping population (‘Ning7840’/‘Clark’) were closely associated with scab resistance in both validation populations. Marker‐assisted selection for the QTL with the SSR markers combined with phenotypic selection was more effective than selection based solely on phenotypic evaluation in early generations. Marker‐assisted selection of the major QTL during the seedling stage plus phenotypic selection after flowering effectively identified scab resistant lines in this experiment. Near‐isogenic lines for this 3BS QTL were isolated from the F6 generation of the cross ‘Ning7840’/‘IL89‐7978’ based on two flanking SSR markers, Xgwm389 and Xbarc147. Based on these results, MAS for the major scab resistance QTL can improve selection efficiency and may facilitate stacking of scab resistance genes from different sources. 相似文献
16.
Heterosis is an important way to improve yield and quality for many crops. Hybrid rice and hybrid maize contributed to enhanced productivity which is essential to supply enough food for the increasing world population. The success of hybrid rice in China has led to a continuous interest in hybrid wheat, even when most research on hybrid wheat has been discontinued in other countries for various reasons including low heterosis and high seed production costs. The Timopheevii cytoplasmic male sterile system is ideal for producing hybrid wheat seeds when fertility restoration lines with strong fertility restoration ability are available. To develop PCR-based molecular markers for use in marker-assisted selection of fertility restorer lines, two F2 populations derived from crosses R18/ND36 and R9034/ND36 were used to map fertility restoration genes in the two elite fertility restorer lines (R-lines) R18 and R9034. Over 678 SSR markers were analyzed, and markers closely linked to fertility restoration genes were identified. Using SSR markers, a major fertility restoration gene, Rf3, was located on the 1B chromosome in both populations. This gene was partially dominant in conferring fertility restoration in the two restorer lines. SSR markers Xbarc207, Xgwm131, and Xbarc61 are close to this gene. These markers may be useful in marker-assisted selection of new restorer lines with T. timopheevii cytoplasm. Two minor QTL conferring fertility restoration were also identified on chromosomes 5A (in R18) and 7D (in R9034) in two R-lines. 相似文献
17.
Harpinder Singh Randhawa Zlatko Popovic Jim Menzies Ron Knox Stephen Fox 《Euphytica》2009,169(2):151-157
This study was conducted to determine the genetic control of resistance to loose smut caused by Ustilago tritici race T33 in two durum recombinant inbred line populations (DT662 × D93213 and Sceptre × P9162-BJ08*B) and to identify molecular
markers linked to the resistance. Resistance in both populations was controlled by single genes. Two SSR markers were linked
with loose smut resistance in the Sceptre × P9162-BJ08*B population. In DT662 × D93213, two AFLP, two wheat SSRs and one SCAR
markers were linked to resistance. The SCAR marker, 3.2 cM distal to the smut resistance locus (Utd1) on chromosome 5BS, accounted for up to 64% of the variability in disease reaction; the other markers were proximal to Utd1 at genetic distances ranging from 5.9 to 35.9 cM. SSR markers Xgwm234 and Xgwm443 segregated in both crosses suggesting a common resistance gene. The SCAR and SSR markers can be used effectively for marker
assisted selection to incorporate loose smut resistance into durum cultivars. 相似文献
18.
Male and female genetic linkage map of hops, Humulus lupulus 总被引：2，自引：0，他引：2
A male and female linkage map of hop has been constructed using 224 DNA polymorphisms (106 amplified fragment length polymorphisms (AFLPs), three random amplified polymorphic DNAs (RAPDs), one RAPD‐sequence‐tagged‐site (STS), and three microsatellite (STSs) segregating in an F1 population of the English cultivar ‘Wye Target’‐the German male breeding line ‘85/54/15’. Linkage between these loci was estimated using JOINMAP Version 2.0. The final map for the female parent consisted of 110 loci assigned to eight linkage groups covering a distance of 346.7 cM. For the male map, 57 loci could be mapped on nine linkage groups spanning over 227.4 cM. One of these male linkage groups (Gr09‐M) presumably represents the Y chromosome, since all markers assigned (10 AFLPs, three RAPDs and one STS) were closely linked to the male sex (M). Because of their sex‐specific segregation, 10 doubly heterozygous AFLPs spanning a distance of 18.7 cM could be identified as markers describing the X chromosome, which is part of the male and female map. Three STMSs, which had already proved useful in hop genotyping, could be integrated as codominant locus‐specific markers and thus allowed to produce reliable allelic bridges between the female and male counterparts. 相似文献
19.
QTLs for black-point resistance in wheat and the identification of potential markers for use in breeding programmes 总被引：3，自引：0，他引：3
A. Lehmensiek A. W. Campbell M. W. Sutherland P. M. Williamson M. Michalowitz G. E. Daggard 《Plant Breeding》2004,123(5):410-416
Quantitative trait loci (QTLs) for black‐point resistance have been mapped in two doubled haploid‐derived wheat populations, each thought to contain unrelated sources of resistance. In the ‘Sunco’בTasman’‐derived population, QTLs were located on chromosomes 1D, 2B, 3D, 4A, 5A and 7A with each QTL explaining between 4 and 15% of the observed phenotypic variance. QTLs were contributed by both parents. In the ‘Cascades’בAUS1408’‐derived population, QTLs from ‘Cascades’ were identified on chromosomes 2A, 2D and 7A with each QTL explaining between 12 and 18% of the phenotypic variance. Several markers were identified which are promising candidates for use in marker‐assisted selection programmes. If one, two or three of these markers would have been used to select for black‐point resistance in the ‘Sunco’בTasman’ population, then with one marker 34 of 39 resistant lines, with two markers 23 of 32 and with three markers 17 of 32 would have been selected. At the same time, 67 false positives obtained by selecting with one marker are reduced to 24 by selection with two markers and to 11 by selection with three markers. Similarly, if one, two or three markers are used to select for black‐point resistance in the ‘Cascades’בAUS1408’ populations, then with one marker 25 of 31 resistant lines, with two markers 26 of 31 and with three markers 10 of 31 are selected. At the same time, 14 false positives are obtained with one marker are reduced to six by selection with two markers and no false positives are selected using three markers. 相似文献
20.
Molecular mapping of a new gene for resistance to frogeye leaf spot of soya bean in 'Peking' 总被引：5，自引：0，他引：5
Amplified fragment length polymorphism (AFLP) and microsatellite (simple sequence repeat, SSR) techniques were used to map the _RGSpeking gene, which is resistant to most isolates of Cercospora sojina in the soya bean cultivar ‘Peking’. The mapping was conducted using a defined F2 population derived from the cross of ‘Peking’(resistant) בLee’(susceptible). Of 64 EcoRI and MseI primer combinations, 30 produced polymorphisms between the two parents. The F2 population, consisting of 116 individuals, was screened with the 30 AFLP primer pairs and three mapped SSR markers to detect markers possibly linked to RcsPeking. One AFLP marker amplified by primer pair E‐AAC/M‐CTA and one SSR marker Satt244 were identified to be linked to ResPeking. The gene was located within a 2.1‐cM interval between markers AACCTA178 and Satt244, 1.1 cM from Satt244 and 1.0 cM from AACCTA178. Since the SSR markers Satt244 and Satt431 have been mapped to molecular linkage group (LG) J of soya bean, the ResPeking resistance gene was putatively located on the LG J. This will provide soya bean breeders an opportunity to use these markers for marker‐assisted selection for frogeye leaf spot resistance in soya bean. 相似文献