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1.
A sandy soil was amended with different types of sewage sludge (digested, dried, and composted) and pig slurry. The composted sludges displayed higher organic‐matter stability (39–45%) than only digested sludge (26–39%) or digested + dried sludge (23–32%). The microbial biomass of the dried sludge was undetectable. Digested and composted sludges and pig slurry displayed microbial biomasses (12492–13887 µg g?1, 1221–2050 µg g?1, and 5511 µg g?1, respectively) greater than the soil (108 µg g?1). The wastes were applied at seven doses, ranging from 10 to 900 g kg?1. Soils were incubated for 28 days. Substrate‐induced respiration (SIR) was measured for 12 consecutive hours on day 1 and on day 28. The results showed that SIR increased with the dose of organic amendment. However, SIR decreased when moderate doses of pig slurry or high doses of digested + dried sludge were tested. The possibility of using this inhibition as an ecotoxicological indicator is discussed.  相似文献   

2.
Abstract

The effect of zinc–phosphorus (Zn‐P) interaction on Zn efficiency of six wheat cultivars was studied. The higher dry matter yields were observed when Zn was applied at 5 µg g?1 soil than with no Zn application. Phosphorus applications also increased dry matter yield up to the application of 25 µg P g?1 soil. The dry matter yield was significantly lower at the P rate of 250 µg g?1 soil. At the Zn‐deficient level, the Zn‐efficient cultivars had higher Zn concentrations in the shoots. Zinc concentrations in all cultivars increased when the P level in the soil was increased from 0 to 25 µg P g?1 soil except for the cv. Durati, in which Zn concentrations decreased with increases in P levels. However, when Zn×P interactions were investigated, it was observed that at a Zn‐deficient level, Zn concentrations in the plant shoot decreased with each higher level of P, and more severe Zn deficiency was observed at P level of 250 µg g?1 soil.  相似文献   

3.
Abstract

To clarify the mechanism of Magnesium (Mg) in alleviating cadmium (Cd) phytotoxicity, Japanese mustard spinach (Brassica rapa L. var. pervirdis) was grown for 10 days after treatment in hydroponics in a growth chamber under natural light. The treatments were: (1) nutrient solution alone (Control), (2) 10 mmol L?1 Mg (High-Mg), (3) 2.5 µmol L?1 Cd (Cd-toxic), (4) 2.5 µmol L?1 Cd plus 10 mmol L?1 Mg (Mg-alleviated). The Cd-toxic treatment showed substantial growth retardation and chlorosis of young leaves, such symptoms were not observed in Mg-alleviated plants. Magnesium-alleviated plants showed higher shoot growth, more than twofold, and decreased shoot Cd concentration, approximately 40%, compared with Cd-toxic plants. This increase in shoot growth and simultaneous decrease in shoot Cd concentration may explain the alleviation of Cd toxicity with Mg in Japanese mustard spinach. In Cd-toxic plants, concentrations of K in shoots and Zn in both shoots and roots increased compared with the other three treatments. Concentrations and accumulations of Fe and Mn in shoots decreased significantly in the Cd-treated (Cd-toxic and Mg-alleviated) plants compared with the control and High-Mg plants. Thus, the application of high amounts of Mg in the nutrient solution can alleviate Cd toxicity in plants.  相似文献   

4.
《Journal of plant nutrition》2013,36(5):1065-1083
Abstract

Ten cvs. of four Brassicaceae species were tested to evaluate their copper (Cu) uptake and translocation. Germination and root length tests indicated that Brassica juncea cv. Aurea and Raphanus sativus cvs. Rimbo and Saxa were the species with the highest germinability and longest roots at Cu concentrations ranging from 25 up to 200 µM. Raphanus sativus cv. Rimbo grown in hydroponic culture at increasing Cu concentrations (from 0.12 up to 40 µM) for 10 days produced a relatively high biomass (17.2 mg plant?1) at the highest concentration and had a more efficient Cu translocation (17.8%) in comparison with cvs. Aurea and Saxa. The potential of cv. Rimbo for Cu uptake was then followed for 28 days at 5, 10, and 15 µM Cu. In comparison with the control, after 28 days of growth the 15 µM Cu‐treated plants showed a reduction in the tolerance index (?40%) and in the above‐ground dry biomass (?19%). On the contrary, an increase in the below‐ground dry weight was observed (+35%). Copper accumulated during the growth period both in the below‐ and above‐ground parts (about 14 and 4 µg plant?1 at 10 and 15 µM Cu, respectively), but the translocation decreased from 50 to 30% in the last week at all the concentrations used. In addition, cv. Rimbo grown in a multiple element [cadmium (Cd), chromium (Cr), Cu, lead (Pb), and zinc (Zn)] naturally‐contaminated site accumulated all elements in the above‐ground part in a range from 5 to 62 µg plant?1.  相似文献   

5.
The genus Prosopis is a tree or shrub in the leguminosae family, subfamily fabaceae (mimosaceae). Many plants of the genus Prosopis are known to have medicinal properties. Only one species of Prosopis is found in Jordan, Prosopis farcta (Banks & Sol.) J.F. Macbr. The local name is Yanbout, and the English name is locust pods.

The aim of this study was to investigate some selected heavy metals including cadmium (Cd), copper (Cu), lead (Pb), and zinc (Zn) in Prosopis farcta, an unexplored Jordanian species of the Prosopis genus, because no data are available about these levels in this medicinal plant. These metals were tested in different parts of Prosopis farcta including root, aerial, and fruit as ethanolic extract plant and dry plant. Moreover, these metals were investigated in soil samples collected from the same area in which Prosopis farcta was grown. Results revealed that there was a significant difference between root and fruit for all test elements (P < 0.05). Roots were found to contain high average concentrations of Pb (2.14 µg g?1), Cu (18.56 µg g?1), and Zn (13.74 µg g?1). Copper and Zn concentrations in Prosopis farcta were within the permissible limits, whereas Pb concentration exceeded the permissible limit. Moreover, soil samples were analyzed for the metals. Results revealed that there was a positive correlation between the levels of Cu and Zn in medicinal plants and soils, whereas there was a negative correlation for Pb.

Two certified reference materials (tea leaves, NCSDC 73351; soil, GBW 07406) were analyzed to authenticate the accuracy of the method, and the precision was expressed by relative standard deviation.  相似文献   

6.
Silicon has the potential to improve drought tolerance in crops. Seeds primed with silicon were used in the present study to explore its potential benefit to withstand water stress. Seeds of two wheat varieties, NARC-2009 and Chakwal-50, were sown in pots after priming with distilled water and different concentrations (0.5%, 1.0% and 1.5%) of silicon sources (silicic acid, sodium silicate and silica gel) at PMAS, Arid Agriculture University, Rawalpindi. Maximum silicon uptake at three-leaf stage (0.028 µg g?1 dry weight (DW)), anthesis (0.072 µg g?1 DW) and maturity (0.103 µg g?1 DW) was recorded for silica gel. Silicon uptake increased significantly in response to increase in Si concentration from 0.5% to 1.5%. Leaf membrane stability index, epicuticular wax, relative water content and proline remained maximum – 78.90%, 2.6 mg g?1 DW, 83.88% and 54.90 µg g?1 – for silica gel treatments compared with others. Silica gel with 1.5% silicon concentration resulted in maximum spike length (14.3 cm), biological yield (7.63 g pot?1), hundred-grain weight (3.97 g pot?1) and grain yield (2.46 g pot?1). Based on the study outcomes, it is concluded that silica gel might be a good priming option with 1.5% silicon concentration to establish plant under drought stress.  相似文献   

7.
Response of the integrated use of mycorrhiza, inorganic and organic manures on microbial variables and yield performance of colocasia (Colocasia esculenta L.) was studied in an acid Alfisol. Significantly highest mean cormel yield (14.13 t ha?1) was recorded due to application of super optimal doses of nitrogen, phosphorus, and potassium (NPK). Integrated application of lime + farmyard manure (FYM) + ½ NPK + zinc sulfate (ZnSO4) has recorded the highest dehydrogenase activity (2.048 µg TPF hr?1 g?1) and Fluorescein Diacetate Hydrolysis assay (1.855 µg g?1 hr?1). The highest acid and alkaline phosphatase activities (77.67 and 51.18 µg PNP g?1 h?1, respectively) were observed due to Vesicular Arbuscular Mycorrhiza (VAM) inoculation in combination with lime + FYM + ½ NPK. Dehydrogenase and phosphatase activities had a highly significant relationship with cormel yield and the biochemical constituents of colocasia. The study emphasized the conjunctive use of balanced inorganic fertilizers and organic manures to enhance the enzymatic activities and to realize higher crop yields of colocasia in acid Alfisols.  相似文献   

8.
Cassava is an important subsidiary food in the tropics. In Tamil Nadu, India, microbial cultures were used to eradicate the tuberous root rot of cassava. Hence, an experiment was conducted for two consecutive years to test the effects of coinoculation of microbes on soil properties. The surface soil from the experimental site was analysed for soil available nutrients, soil enzyme activities and microbial biomass carbon. The treatment of Azospirillum with Trichoderma at the 50% recommended N:P2O5:K2O (NPK) rate (50:25:50 kg ha?1) significantly increased soil available nitrogen (142.81 kg ha?1) by 72.66% over uninoculated control. There was a significant increase in available phosphorus in soil by the inoculation of AM (arbuscular mycorrhizal) fungi with Trichoderma at the 50% recommended NPK rate (41.04 kg ha?1) compared to other treatments. The application of Pseudomonas fluorescens with Trichoderma at the 50% recommended NPK rate significantly increased available iron (19.34 µg g?1) in soil. The treatment of Azospirillum with Trichoderma increased urease enzyme activity at the recommended NPK rate (816.32 μg urea hydrolyzed g?1 soil h?1). Soil application of all cultures at the 50% recommended NPK rate significantly increased dehydrogenase activity (88.63 μg TPF g?1 soil) and β-glucosidase activity (48.82 μg PNP g?1 soil) in soil. Inoculation of Trichoderma alone at the 50% recommended NPK rate significantly increased microbial biomass carbon (3748.85 μg g?1 soil). Thus, the microbial inoculations significantly increased soil available nutrient contents, enzyme activities such as urease, dehydrogenase and β-glucosidase activity and microbial biomass carbon by reducing the amount of the required fertilizer.  相似文献   

9.
Abstract

Six desulfo-glucosinolates (DS-GSLs), DS-glucoraphanin, DS-4-(β-d-glucopyranosyldisulfanyl)butyl GSL, DS-glucoerucin, DS-glucobrassicin, DS-dimeric 4-mercaptobutyl GSL and DS-4-methoxyglucobrassicin, were isolated from rocket salad (Eruca sativa Mill.) By using high-pressure liquid chromatography (HPLC) and identified by their retention times on HPLC and electrospray ionization mass spectrometry (ESI-MS) techniques. Total GSL contents were 125, 11 and 22 µmol g?1 dry weight (DW) in seeds, leaves and roots, respectively. Two DS-GSLs, namely DS-glucoraphanin and DS-glucoerucin, were the major GSLs in seeds and roots, while DS-glucoraphanin, DS-glucoerucin and DS-dimeric 4-mercaptobutyl GSL were the predominant GSLs in leaves (> 1 µmol g?1 DW or > 10% of the total GSL content). In addition, the anti-oxidant activity (n = 3) of intact leaf and root powders, whole DS-GSL eluates and purified DS-4-methoxyglucobrassicin were measured using the XYZ-dish method. DS-4-methoxyglucobrassicin showed the greatest anti-oxidant activity value (3.8 unit g?1 DW), and the value for whole DS-GSL eluates (1.6) was higher than that of intact leaves (1.5) and roots (0.87).  相似文献   

10.
We assessed the effect of mycorrhizal inoculation on short‐term uptake kinetics of arsenate and arsenite by excised roots of upland rice (Oryza sativa L. cv. Zhonghan 221). A concentration of 0.01–0.05 mM arsenic (As) differentially affected the influx rates of both arsenate and arsenite into rice roots non‐inoculated or inoculated with Glomus mosseae and G. versiforme. While Vmax for arsenate uptake by non‐mycorrhizal roots was 1.02 µmol g?1 fresh weight h?1, it was reduced by a factor of 2.4 for mycorrhizal roots (about 0.42 µmol g?1 fresh weight h?1) in the high‐affinity uptake system. However, at high concentrations of 0.5–2.5 mM As only G. versiforme was able to reduce As influx. The results show that mycorrhizal effects on As uptake of upland rice are both concentration and species‐specific.  相似文献   

11.
The effect of medium-term (5 years) application of organic and inorganic sources of nutrients (as mineral or inorganic fertilizers) on soil organic carbon (SOC), SOC stock, carbon (C) build-up rate, microbial and enzyme activities in flooded rice soils was tested in west coast of India. Compared to the application of vermicompost, glyricidia (Glyricidia maculate) (fresh) and eupatorium (Chromolaena adenophorum) (fresh) and dhaincha (Sesbania rostrata) (fresh), the application of farmyard manure (FYM) and combined application of paddy straw (dry) and water hyacinth (PsWh) (fresh) improved the SOC content significantly (p < 0.05). The lowest (p < 0.05) SOC content (0.81%) was observed in untreated control. The highest (p < 0.05) SOC stock (23.7 Mg C ha?1) was observed in FYM-treated plots followed by recommended dose of mineral fertilizer (RDF) (23.2 Mg C ha?1) and it was lowest (16.5 Mg C ha?1) in untreated control. Soil microbial biomass carbon (Cmb) (246 µg g?1 soil) and Cmb/SOC (1.92%) were highest (p < 0.05) in FYM-treated plot. The highest (p < 0.05) value of metabolic quotient (qCO2) was recorded under RDF (19.7 µg CO2-C g?1 Cmb h?1) and untreated control (19.6 µg CO2-C g?1 Cmb h?1). Application of organic and inorganic sources of nutrients impacted soil enzyme activities significantly (p < 0.05) with FYM causing highest dehydrogenase (20.5 µg TPF g?1 day?1), phosphatase (659 µg PNP g?1 h?1) and urease (0.29 µg urea g?1 h?1) activities. Application of organic source of nutrients especially FYM improved the microbial and enzyme activities in flooded and transplanted rice soils. Although the grain yield was higher with the application of RDF, but the use of FYM as an organic agricultural practice is more useful when efforts are intended to conserve more SOC and improved microbial activity.  相似文献   

12.
Abstract

A study was undertaken to determine the effects of different concentrations of arsenic (As) in irrigation water on Boro (dry‐season) rice (Oryza sativa) and their residual effects on the following Aman (wet‐season) rice. There were six treatments, with 0, 0.1, 0.25, 0.5, 1, and 2 mg As L?1 applied as disodium hydrogen arsenate. All the growth and yield parameters of Boro rice responded positively at lower concentrations of up to 0.25 mg As L?1 in irrigation water but decreased sharply at concentrations more than 0.5 mg As L?1. Arsenic concentrations in grain and straw of Boro rice increased significantly with increasing concentration of As in irrigation water. The grain As concentration was in the range of 0.25 to 0.97 µg g?1 and its concentration in rice straw varied from 2.4 to 9.6 µg g?1 over the treatments. Residual As from previous Boro rice showed a very similar pattern in the following Aman rice, although As concentration in Aman rice grain and straw over the treatments was almost half of the As levels in Boro rice grain. Arsenic concentrations in both grain and straw of Boro and Aman rice were found to correlate with iron and be antagonistic with phosphorus.  相似文献   

13.
Abstract

We have identified Crassocephalum crepidioides (Benth.) S. Moore (Compositae) as a cadmium (Cd)-accumulator plant in a heavy-metal polluted environment. In soil polluted with Cd, 5.7–17.5 mg kg?1 Cd, concentrations in the above-ground plant tissues were measured as 14.6–78.6 mg kg?1 with transfer factors in the above-ground plant tissues (concentration in above-ground tissues/soil concentration) of 1.5–6.0. No other toxic heavy metals or plant micronutrients were found to have accumulated into the above-ground plant tissues. In a hydroponic culture with 1 µmol L?1 Cd added to Hoagland's nutrient solution, Cd concentration in the above-ground plant tissues was 121.0 mg kg?1, with a transfer factor of more than 1000. In a pot culture carried out for 9 weeks in a greenhouse, the highest Cd concentration in the above-ground plant tissues, 121.2 mg kg?1, was found in a treatment with 5 mg kg?1 Cd, whereas the highest Cd content in an above-ground plant tissue, 106.1 µg, was found in a treatment with 2 mg kg?1 Cd. These results clearly showed that C. crepidioides is a Cd accumulator. In all samples, the Cd concentration in the above-ground plant tissues was higher than that in the roots. The results obtained in the present study show that this plant has a strong potential for use in phytoremediation in farm fields contaminated with Cd.  相似文献   

14.
Abstract

Arsenic (As) is a deadly poison at high concentrations. It is mysterious in the sense that people are exposed to it most of the time through drinking groundwater, fortunately at much lower concentrations than the deadly levels, and usually without knowing it. Arsenic content in alluvial aquifers of Punjab varied from 3.5 to 688 µg L?1. Arsenic status of groundwater is classified into low (<10 µg L?1), moderate (≥10 to <25 µg L?1), high (≥25 to <50 µg L?1), and very high (>50 µg L?1). In zone I, the concentration of As in groundwater varied from 3.5 to 42 µg L?1 with a mean value of 23.4 µg L?1. On the basis of these limits, only 8% of samples were low, whereas 51 and 41% of the total samples collected from this region fall in the moderate and high As categories. The concentration of As in groundwater of zone II varied from 9.8 to 42.5 µg L?1 with a mean value of 24.1 µg L?1. Arsenic concentration in the alluvial aquifers of the central plain of zone II is 2 and 52% in the low and moderate limits. In this region, 46% of groundwater sites contain high As concentrations. Arsenic concentrations in the aridic southwestern parts are significantly different from other two provinces. The As concentration ranged from 11.4 to 688 µg L?1 with average value of 76.8 µg L?1. Eleven percent of the aquifers of the southwestern region of zone III are in the moderate category, 54% in the high, and 35% in the very high. According to safe As limits (<10 µg L?1), only 3 and 1% of the groundwater samples collected from zones I and II were fit for dinking purposes with respect to As content. In the aridic southwest, zone III, all water samples contained As concentrations greater than the safe limits and thus are not suitable for drinking purposes. The presence of elevated As concentrations in groundwater are generally due to the results of natural occurrences of As in the aquifer materials. The concentration of other competitive oxyanions in waters such as phosphate, sulfate, and borate also depressed the adsorption of As on the sorption sites of aquifer materials and thereby eventually elevate the As concentration in groundwaters. In groundwater of alluvial aquifers of Punjab, released from sulfide oxidation and oxyhydroxide of iron, elevated (>10 µg L?1) concentrations of As were widespread because of high pH (>8.0) and higher concentrations of phosphate, borate, sulfate, and hydroxyl anions. It is conclusively evident that geochemical conditions, such as pH, oxidation–reduction, associated or competing ions, and evaporative environments have significant effects on As concentration in groundwater. These conditions influence how much As is dissolved or precipitated into the water and how much is bound to the aquifer materials or the solid particles in water.  相似文献   

15.
Abstract

Maximum yield research (MYR) and maximum economic yield systems (MEY) are important under the present situation of increased demographic changes, which have reduced the per capita availability of both land and food. Among root and tuber crops, cassava is found in a variety of production systems and performs well under various levels of management from low‐input to high‐input systems. A systematic approach in fertilizer use involves the determination and elimination of soil nutrient constraints for balanced supply of all potentially deficient essential nutrients for sustainable high yield. In the systematic approach of determining optimal fertilizer for cassava (Manihot esculenta Crantz) in a Typic Kandiustult soil of Kerala, India, studies on critical levels of nutrients, original nutritional status of the soil, its sorption capacity, and greenhouse/screenhouse nutrient survey were carried out. The critical levels of phosphorus (P) and potassium (K) were determined as 8.23 and 43.5 µg g?1, respectively. The preliminary analysis of the soil of the experimental site revealed the overall fertility status as very poor. The sorption studies conducted for P, K, copper (Cu), zinc (Zn), manganese (Mn), sulfur (S), and boron (B) indicated P, K, S, and B were limiting in this particular soil. The quantities of these nutrients needed to optimize the soil nutrient status were determined from the preliminary soil analysis, critical levels of nutrients, and sorption curves and found to be 136 µg mL?1 P, 0.338 meq 100 mL?1 K, 20 µg mL?1 S, and 6.025 µg mL?1 B, respectively. From the greenhouse nutrient survey, N, P, K, and calcium (Ca) were seen as limiting nutrients for this soil; therefore the optimum treatment for cassava was fixed as N‐P‐K at 100:300:300 kg ha?1, respectively. Field experiments were conducted for two seasons with different levels of the optimum treatment using a short duration (6–7 months) cassava variety, Sree Vijaya. The optimum treatment gave a yield of 43.41 t ha?1, whereas the yield under the existing practice recommendations was 20–25 t ha?1. Economic analysis showed this optimum treatment as the best in terms of highest gross return (Rs 1,31,125/‐), net return (Rs 83,150/‐), added return (Rs 70, 178/‐), added profit (Rs 56,142/‐), and benefit cost ratio (BCR) (2.73).  相似文献   

16.
The concentrations of Hg, Cu, Pb, Cd, and Zn accumulated by regional macrophytes were investigated in three tropical wetlands in Colombia. The studied wetlands presented different degrees of metal contamination. Cu and Zn presented the highest concentrations in sediment. Metal accumulation by plants differed among species, sites, and tissues. Metals accumulated in macrophytes were mostly accumulated in root tissues, suggesting an exclusion strategy for metal tolerance. An exception was Hg, which was accumulated mainly in leaves. The ranges of mean metal concentrations were 0.035?C0.953 mg g?1 Hg, 6.5?C250.3 mg g?1 Cu, 0.059?C0.245 mg g?1 Pb, 0.004?C0.066 mg g?1 Cd, and 31.8?363.1 mg g?1 Zn in roots and 0.033?C0.888 mg g?1 Hg, 2.2?C70.7 mg g?1 Cu, 0.005?C0.086 mg g?1 Pb, 0.001?C0.03 mg g?1 Cd, and 12.6?C140.4 mg g?1 Zn in leaves. The scarce correlations registered between metal concentration in sediment and plant tissues indicate that metal concentrations in plants depend on several factors rather than on sediment concentration only. However, when Cu and Zn sediment concentrations increased, these metal concentrations in tissues also increased in Eichhornia crassipes, Ludwigia helminthorriza, and Polygonum punctatum. These species could be proposed as Cu and Zn phytoremediators. Even though macrophytes are important metal accumulators in wetlands, sediment is the main metal compartment due to the fact that its total mass is greater than the corresponding plant biomass in a given area.  相似文献   

17.
《Journal of plant nutrition》2013,36(7):1227-1242
Abstract

Uptake of arsenic (As) and its distribution in Chinese Brake fern (Pteris vittata L.), an As hyperaccumulator, and Boston fern (Nephrolepis exaltata L.), a nonhyperaccumulator, in the presence of phosphorus (P), were characterized by employing a hydroponic experiment with a complete three-factorial design. Two levels of As (100 and 1000 µM) and four levels of P (0, 100, 500, and 1000 µM) were used in this study. Arsenic uptake rates on the basis of root fresh weight for the two ferns were similar at low As concentration (100 µM). At high As concentration (1000 µM), however, As uptake rates (373–987 nmol g?1 f wt h?1) of P. vittata were significantly greater than those of N. exaltata (164–459 nmol g?1 f wt h?1). In both ferns, addition of P reduced their As uptake rate as well as accumulation. Pteris vittata had a greater As TF (Translocation factor = concentration ratio of fronds to roots) than N. exaltata. On the contrary, N. exaltata displayed a greater P TF than P. vittata. As a result, high P/As ratio was observed in the roots of P. vittata, whereas high P/As ratio was observed in the fronds of N. exaltata. The study illustrated that As hyperaccumulation by P. vittata may be facilitated by its high As influx rate and its high molar P/As ratio in the roots resulting from both high As TF and low P TF.  相似文献   

18.
The phytotoxicity imposed by cadmium (Cd) in sunflower was investigated on biomass, Cd accumulation, superoxide and lipid peroxidation product as well as the activities of superoxide dismutase, catalase and peroxidase. Plants accumulate substantial amount of Cd in different parts, the maximum being in roots, i.e., up to 820 μg g?1 dry matter. Cadmium induced oxidative stress, indicated by increase in lipid peroxidation and superoxide content with increase in metal supply. Under Cd stress, the activities of superoxide dismutase and catalase declined to a greater extent in roots than in leaves. Even though the peroxidase activity increased in leaves, a decreasing trend was observed in root due to Cd stress. The threshold of toxicity (10% growth reduction) and toxicity (33% growth reduction) values of Cd in sunflower were 14 and 72 μg g?1 in leaves, 19 and 90 μg g?1 in stem and 65 and 250 μg g?1 Cd in roots, respectively.  相似文献   

19.
Abstract

A series of laboratory incubation experiments were conducted on soils from Maindample and Ruffy in northeast Victoria and from Whittlesea in the Plenty Valley, north of Melbourne, Victoria, Australia, to develop a technique for quantifying both autotrophic and heterotrophic nitrification in acidic pasture soils. The use of a specific inhibitor of the autotrophic ammonium oxidizers (N‐serve) did not completely inhibit autotrophic nitrification in its commonly recommended concentrations (10 and 20 µg g?1 soil) in these soils. The N‐serve concentration, which completely inhibited autotrophic nitrification, was found to be 60–80 µg g?1. Varying soil types, pHs, and organic‐matter contents affected the optimum dose of N‐serve required for complete inhibition of autotrophic nitrification. Mixing the inhibitor with the soil after application was also important for immediate inhibition of autotrophic nitrification. Using N‐serve in combination with 15N‐labeled glycine in the Maindample soil showed that heterotrophic organisms were using the organic route for nitrification, and N‐serve did not affect heterotrophic nitrification. A lag of 12 to 24 h in complete inhibition of autotrophic nitrification by N‐serve may have occurred suggesting nitrification studies using N‐serve should include pre‐incubation of the soils with N‐serve for at least 1 day.  相似文献   

20.
Abstract

Nutrient sorption studies were carried out as a part of a mega project on “Yield maximization in cassava (Manihot esculenta Crantz) through systematic approach in fertilizer use” to find out the sorption characteristics of major, secondary, and micronutrients [i.e., phosphorus (P), potassium (K), sulfur (S), copper (Cu), zinc (Zn), manganese (Mn), and boron (B)] in a typic kandiustult of Kerala, India. The sorption curve fitted by using this study along with the results of preliminary soil analysis and critical level of these nutrients were used to optimize the nutritional status of this soil. Sorption study revealed that P, K, S, and B were limiting and others were sufficient for this soil. The quantities of the limiting nutrients required to optimize the soil nutritional status were estimated from the sorption curve as P, K, S, and B at 136 µg mL?1, 0.338 meq 100 mL?1, 20 µg mL?1, and 6.025 µg mL?1, respectively.  相似文献   

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