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1.
Abstract

The effect of liming on mineralization and soil nitrifier activity (NA) was investigated with Brookston clay (pH 5.7) and Haldimand clay (pH 4.7). Liming increased the rate of mineralization in both soils but at a rate about 4‐times greater in Haldimand clay than Brookston clay. A significant increase in N mineralization due to liming occurred in both soils only when pH was raised above 6.0. The rate of mineralization was greater than nitrification in the Haldimand soil resulting in NH4 + accumulation. Nitrifier activity increased with liming of Brookston clay, but decreased in Haldimand clay after 15 days of incubation. There was a significant increase in nitrifier activity due to liming from 15 to 60 days in Haldimand clay. After 60 days nitrifier activity in limed treatments increased by five times over the unlimed control.

The nitrification of urea powder (1000 mg N.kg‐1) mixed into the soil was also studied in several soils incubated at 15°C for 28 days. There was evidence up to 14 days that nitrification of urea was correlated with initial nitrifier activity. Between 14 and 28 days, other factors such as soil pH and possible ammonia toxicity in coarser textured soils as well as nitrifier activity were important. Accumulation of nitrite occurred mainly in soils with a pH above 7.0 up to 28 days especially where nitrifier population enrichment was not done.  相似文献   

2.
The absorption and assimilation of 15N‐labeled urea applied to the foliage of tall fescue (Festuca arundinacea Schreb.) and creeping bentgrass (Agrostis palustris Huds.) turf was examined under a controlled environment. Each source of N was dissolved in deionized water to a final concentration of 25 g N liter‐1 and spray‐applied at a rate of 5 g N m‐2. Absorption of the fertilizer‐N over a 72 hr period, as measured by 15N analysis of tissue digests, averaged 55% for the two species. Absorption was also estimated by a washing procedure which measured the urea remaining on the foliage, and by the increase in total N in the plant tissue.

There were no significant differences between the three methods in estimating absorption. Partitioning of the absorbed 15n between tissues averaged 37% in new leaves, 51% in old leaves and shoot tissue, and 11% in the roots. More than 90% of the absorbed urea‐N was hydrolyzed by 72 hr.  相似文献   

3.
Application of 10 mM urea to the flag leaf of wheat plants enhanced in vivo urease activity several fold. Photosynthetic rate was also increased considerably. There were significant differences in the leaf internal carbon dioxide (CO2) concentrations between the urea‐treated and untreated leaves. The finding that carbon (14C) was detected in the ethanol extract of the leaves fed with 14C‐urea suggests that CO2 released from urea is re‐fixed by the leaves.  相似文献   

4.
Abstract

A laboratory study was conducted to assess the effectiveness of phenylphosphorodiamidate (PPD) and N‐(n‐butyl) thiophosphoric triamide (NBT) in retarding urea hydrolysis in four flooded rice soils under simulated oxidized and reduced conditions. Urea (400 μg N g‐1soil) with PPD or NBT (2.0% w/w) was added to preincubated soils and analyzed for urea content 1, 3, 5, 7 and 15 days after N application. N‐(n‐butyl) thiophosphoric triamide was more effective in delaying urea hydrolysis under oxidizing conditions and at 5 days 57% of the added urea remained in the oxidized soils compared to only 4% under reduced soil conditions. In three soils, PPD was observed to be effective under reducing soil constraints. At 5 days 56 and 31% of the added urea was unhydrolyzed under reducing and oxidizing soil conditions, respectively, with the addition of PPD. For two soils 48% of the added urea remained at the 15 day sampling for the urea + NBT treatment  相似文献   

5.
Controlled‐release urea is a fertilizer which meters out urea over a long period of time. It can provide a favorable nitrogen (N) concentration for root growth, especially at the early stage of plant development. The objective of this study was to determine the interactions of urea or controlled‐release urea granules with barley roots and the resultant N uptake by plants. Two experiments (Experiment I and Experiment II) with treatments of Nil, non‐coated urea, Coated I and Coated II (Coated I and Coated II are controlled‐release urea products) were conducted in a greenhouse at 23±5°C. In both experiments, one barley (Hordeum vulgare L. cv. Duke) seed and one granule of urea or controlled‐release urea were placed in a pot (5.2‐cm height and 8‐cm diameter) containing soil low in mineral N. In Experiment I, shoot and soil samples were taken at 14, 28, and 46 days after seeding. Roots and fertilizer interaction were visually examined and photographed. In Experiment II, root samples both around the fertilizer granule and away from the granule were taken only at 28 days after seeding. In both experiments, dry matter mass and total N content of shoot and root, and mineral N in soil were determined. In Experiment I, at the 28‐day sampling roots proliferated around the controlled‐release urea granule but not around the urea granule. Shoot N uptake since the 28 days was higher with controlled‐release urea than with urea because of the root proliferation. In Experiment II, root dry mass and N content around the granule was higher with controlled‐release urea than with urea. In the controlled‐release urea treatments, root mass and N content away from the granule were also increased in comparison to the Nil. This shows a stimulus relationship between the two portions of the roots in the same plant, i.e., the roots being accessed to the N source increased growth of the other roots with no access to the source. Because only a small portion of roots was involved in N uptake in the controlled‐release urea treatments, the intensity of N uptake per unit of root mass was much higher with controlled‐release urea as compared to urea. In conclusion, root growth was enhanced around controlled‐release urea granule, and that portion of roots around the fertilizer granule played a major role in absorbing N. In addition, a stimulus relationship existed between roots grown around the granule and those grown away from the granule.  相似文献   

6.
Effects of autumn foliar application of N‐urea on N storage and reuse in young apple seedling (Malus Pumila Mill.) were studied. Foliar application of urea‐15 N (3.5%) during autumn enhanced the retranslocation of leaf N to other plant parts and increased stored N. Foliar N sprays increased the proteolytic activities of the leaves; therefore, such activities appear to be a major mechanism of retranslocation of leaf N. Foliar applied N enabled the plant to produce more growth during the following seasons. A considerable portion of the plant total N during second and third years was attributable to the foliarly applied N during the first year.  相似文献   

7.
Abstract

Large volatile losses of NH3 can occur from surface‐applied urea in semi‐arid areas. Our objective was to determine possible absorption of this volatilized N by the crop canopy under field conditions. At two different times during crop growth, 15N‐enriched urea was surface‐applied at rates equivalent to 100 kg N ha‐1 to soil contained in trays placed between two rows of spring wheat. Seven days after application, the soil in the trays was removed from the field and analyzed for 15N content. Addition of HC1 during soil air drying was necessary to prevent volatile losses of 15N. Of applied urea‐N, 13% was volatilized over seven days at both application times. Of the urea‐N that was volatilized, 15% was absorbed by wheat at the first application time and 7% was absorbed by wheat at the second application time. Plant absorption of urea N (Y, mg) declined with distance from the source (x, cm) following the equation Y=10.95*10(‐0.0142x). About 90% of absorbed N was within the first three wheat rows. Our findings suggest that a significant portion of ammonia volatilized from top‐dressed urea might be captured by plant foliage.  相似文献   

8.
Abstract

Dicyandiamide (DCD) is a nitrification inhibitor that has been proposed for use in drill‐seeded rice. Immobilization of fertilizer NH4 +‐N by soil microorganisms under aerobic conditions has been found to be significantly enhanced in the presence of a nitrification inhibitor. The objective of this laboratory study was to determine if DCD significantly delayed nitrification of urea‐derived N, and if this enhanced immobilization of the fertilizer N in the delayed‐flood soil system inherent to dry‐seeded rice culture. Nitrogen‐15‐labeled urea solution, with and without DCD (1: 9 w/w N basis), was applied to a Crowley silt loam (Typic Albaqualf) and the soil was incubated for 10 weeks in the laboratory. The soil was maintained under nonflooded conditions for the first four weeks and then a flood was applied and maintained for the remaining six weeks of incubation. The use of DCD significantly slowed the nitrification of the fertilizer N during the four weeks of nonflooded incubation to cause the (urea + DCD)‐amended soil to have a 2.5 times higher fertilizer‐derived exchangeable NH4+‐N concentration by the end of the fourth week. However, the higher exchangeable NH4+‐N concentration had no significant effect on the amount of fertilizer N immobilized during this period. Immobilization of the fertilizer N appeared to level off during the nonflood period about the second week after application. After flooding, immobilization of fertilizer N resumed and was much greater in the (urea + DCD)‐amended soil that had the much higher fertilizer‐derived exchangeable NH4 +‐N concentration. Immobilization of fertilizer N appeared to obtain a maximum in the urea‐amended soil (18%) about two weeks after flooding and for the (urea + DCD)‐amended soil (28%) about four weeks after flooding.  相似文献   

9.
Abstract

Field experiments were conducted for periods of 14 or 15 years at four sites on Thin Black Chernozemic soils in south‐central Alberta to determine the effect of source and time of N application on dry matter yield (DMY), protein yield (PY), protein concentration, N use efficiency and recovery of N applied to bromegrass (Bromus inermis Leyss.) grown for hay. Two sources of N (urea and ammonium nitrate ‐ A.N.) were applied at four times (early fall, late fall, early spring and late spring) at a rate of 112 kg N ha‐1. Urea was generally less effective in increasing DMY, PY, protein concentration, N use efficiency and % N recovery than A.N. The average, DMY, PY, protein concentration, N use efficiency and % N recovery with A.N. were 4.38 t ha‐1, 445 kg ha‐1, 104 g kg‐1, 21.2 kg DM kg‐1 N ha‐1 and 40.2%, respectively. In the same order, the values with urea were 3.90 t ha‐1, 376 kg ha‐1, 99 g kg‐1, 16.9 kg DM kg‐1 N ha‐1 and 30.2%, respectively. The DMY was greatest with early spring application for A.N., while the protein concentration, PY and % N recovery were greatest with the late fall application for both urea and A.N. The increase in DMY or recovery of applied N with urea as a percentage of the increase with A.N., was greatest with application in late spring and least with application in early fall. In conclusion, urea was less effective than A.N. as a forage fertilizer and early spring application was most effective for increasing DMY.  相似文献   

10.
Abstract

Work reported showed that N‐butyl phosphorothioic triamide (NBPT) is considerably more effective than phenylphosphorodiamidate (PPD) as a soil urease inhibitor and merits consideration as a fertilizer amendment for retarding hydrolysis of urea fertilizer in soil. Studies to determine the factors influencing the effectiveness of NBPT for retardation of urea hydrolysis in soil showed that the inhibitory effect of NBPT on hydrolysis of urea by soil urease increased markedly with the amount of NBPT added and decreased markedly with time and with increase in temperature from 10 to 40°C. They also showed that the ability of NBPT to retard urea hydrolysis in 13 surface soils selected to obtain a wide range in properties was significantly correlated with organic C content (r = ‐0.70**), total N content (r = ‐0.76**), cation‐exchange capacity (r = ‐0.67* ), sand content (r = 0.61*), clay content (r = ‐0.63*), and surface area (r = ‐0.66*), but was not significantly correlated with pH, silt content, urease activity, or CaCO3 equivalent. Multiple‐regression analyses indicated that the effectiveness of NBPT for retardation of urea hydrolysis in soil tends to increase with decrease in soil organic‐matter content.  相似文献   

11.
Growth and N‐P‐K uptake in pumpkin (Curcubita moschata Poir.) cv ‘Libby‐Select’ were studied in dryland and irrigated culture. In both moisture regimes, maximum rates of dry matter accumulation occurred between the early and mid‐fruiting developmental stages. Higher total dry matter production with irrigated than dryland culture was primarily associated with increased shoot growth. Concentrations of N, P, and K in foliage generally decreased as pumpkin age increased. Irrigated pumpkins in conjunction with higher total vegetative dry matter accumulated more N, P, and K than dryland pumpkins. Up through early fruit development, N, P, and K accumulation was primarily in leaves and vines and by the late growth stages was almost entirely in the fruit. Total N, P, and K uptake at late fruiting was estimated at 219, 32, and 228 kg/ha in irrigated pumpkins and 180, 21, and 177 kg/ha in dryland pumpkins. Approximately 58% of the N, 52% of the K, and 68% of the P accumulated by late‐fruiting was absorbed by the plant after the early‐fruiting stage in both moisture regimes. Potassium redistribution from vegetative tissues during late fruit development decreased foliar K contents 32% in dryland pumpkins and 21% in irrigated pumpkins.  相似文献   

12.
Abstract

The objective of this study was to determine the effects of pH and ionic strength on the distribution and speciation of zinc (Zn), copper (Cu), and cadmium (Cd) in surface soil samples from two Brazilian Oxisols amended with biosolids. Soils and biosolids were equilibrated in an experimental dual‐chamber diffusion apparatus that permits the soils and biosolids to react through a solution phase via diffusion across a membrane. After equilibrium was reached, soil and biosolids samples were sequentially fractionated to identify various solid forms of Zn, Cu, and Cd. Metal concentrations in the solution phase were determined and mass balance calculated. Equilibrating pH had no major effect on Cu solubility from biosolids and, at pH range from 4 to 7, most Cu remained in the biosolids. Soluble Zn and Cd concentration increased with decreasing pH because of the increased solubility of the biosolids. Copper and Zn were primarily associated with the residual fraction and Fe oxides in one soil, but were primarily associated with chemically unstable fractions, or adsorbed to the surface of oxides, in the other soil. In both soils, Cd was primarily associated with readily bioavailable fractions. The effect of pH on the metal distribution was more evident than the ionic strength effect. Free ions were the predominant metal species in solution, especially at lower pH values.  相似文献   

13.
Abstract

Distribution of dissolved (DOC) and soil organic carbon (SOC) with depth may indicate soil and crop‐management effects on subsurface soil C sequestration. The objectives of this study were to investigate impacts of conventional tillage (CT), no tillage (NT), and cropping sequence on the depth distribution of DOC, SOC, and total nitrogen (N) for a silty clay loam soil after 20 years of continuous sorghum cropping. Conventional tillage consisted of disking, chiseling, ridging, and residue incorporation into soil, while residues remained on the soil surface for NT. Soil was sampled from six depth intervals ranging from 0 to 105 cm. Tillage effects on DOC and total N were primarily observed at 0–5 cm, whereas cropping sequence effects were observed to 55 cm. Soil organic carbon (C) was higher under NT than CT at 0–5 cm but higher under CT for subsurface soils. Dissolved organic C, SOC, and total N were 37, 36, and 66%, respectively, greater under NT than CT at 0–5 cm, and 171, 659, and 837% greater at 0–5 than 80–105 cm. The DOC decreased with each depth increment and averaged 18% higher under a sorghum–wheat–soybean rotation than a continuous sorghum monoculture. Both SOC and total N were higher for sorghum–wheat–soybean than continuous sorghum from 0–55 cm. Conventional tillage increased SOC and DOC in subsurface soils for intensive crop rotations, indicating that assessment of C in subsurface soils may be important for determining effects of tillage practices and crop rotations on soil C sequestration.  相似文献   

14.
Annual plants may partition carbon (C) preferentially to reproductive structures slowing root elongation and subsequent nutrient uptake. Although foliar applications of nitrogen (N), phosphorus (P), potassium (K), and sulfur (S) supplement uptake by roots, soybean [Glycine max (L.) Merr.] yield increases have not been found in most studies. Experiments were designed to determine if foliar applications of boron (B), magnesium (Mg), or B+Mg would increase soybean yield and if soybean would respond to B applied to the soil several weeks prior to planting. Foliar B or Mg applied separately four times during reproductive growth did not affect soybean yield. However, four foliar applications of B+Mg increased soybean yield 12% at Mt. Vernon and 4% at Columbia over a three‐year period. Two foliar applications of B+Mg during the late reproductive stages increased soybean yield 8% over a two‐year period. The yield increase from foliar B+Mg treatment resulted from an increased number of pods on the main stem (18%) and branches (44%). A 2.8 kg/ha B application to soil eight weeks prior to planting increased soybean yield 11% during the first year and 13% the second year but had no effect on soybean yield by the third year after application. When results from the first two years were combined, 2.8 kg/ha B applied to soil increased the number of pods per branch by 17% and the number of branch pods per plant by 39%. Foliar applications of B+Mg increased soybean yield in four of six site‐years in the three‐year experiments at two locations.  相似文献   

15.
Both enzymatic activities increased under B‐deficient and B‐toxic treatments. The ortho‐diphenolic content did not change with B levels.

Our results suggest that the primary B action on the OPP pathway is at the first enzyme (glucose‐6P‐dehydrogenase) level and that the B effect on 6P‐gluconate‐dehydrogenase is secondary to this. The B action on the enzymatic activities seems not to be caused by any direct interaction with substrates, as B infiltration of B‐deficient culture “in vivo”; seems to result in long term effects on cell structures and/or processes not easily r

In this paper the glucose‐6P‐dehydrogenase and 6P‐gluconate ‐ dehydrogenase activities and ortho‐diphenolic content of hydroponically‐cultivated sunflower‐leaves with moderately deficient, normal and toxic B levels were measured. The change in these parameters during time was considered, together with the restoration of enzymatic activities by means of borate infiltration of deficient and normal leaves. The micronutrient content of the leaves was alsversible by B infiltration of leaves. The positive correlation found between the 6P‐gluconate‐dehydrogenase activity and the Zn content in leaves might be interpretable as and indirect B effect on that activity through modification of Zn content.  相似文献   

16.
Sorghum [Sorghum bicolor (L.) Moench cv RTX430, SC214, SC574, SC599, TAM428, and SC326xSC103] were grown on soils of pH 4.2 or 6.2–6.5. Leaf and nonexserted juvenile panicle tissues were collected at 75 days after planting. Fresh and dry weights were measured and element contents [sulfur (S), phosphorus (P), magnesium (Mg), calcium (Ca), potassium (K), zinc (Zn), iron (Fe), and copper (Cu)] were measured by atomic absorption. Significant cultivar differences in ion concentration (μmol/g dry weight) were found. Juvenile panicles had higher ion concentration (μmol/g dry weight) [S, P, Mg, Ca, K, Zn, and Cu) than leaves. Within leaf tissue, ion concentration (μmol/g dry weight) was correlated with tissue water content (g water/g dry weight).  相似文献   

17.
The suitability of loss‐on‐ignition (LOI) as an alternative to direct measurement of organic carbon (OC) has been debated for decades without resolution. The literature contains an abundance of different linear regression models to describe the LOI–OC relationship, most based on untransformed values of LOI and OC. Such regression is suspect because the variables are unable to occupy Euclidean space. Logratio transformation—based on relative rather than absolute differences—eliminates this constraint. Re‐analysis of the relationship on new and 10 previously published datasets using logratio techniques reveals that the relationship is nonlinear and that the profusion of regression models is in part a function of the range of LOI. Although LOI may offer a crude estimate of OC at high LOI levels, OC/LOI ratios when LOI is less than about 25% are too variable for reliable OC estimation, and interstudy comparisons remain dubious. Direct measurement of OC is recommended.  相似文献   

18.
Abstract

About 35% of soils in Venezuela are acid and low in available phosphorus (P). To solve this problem farmers lime and apply phosphate fertilizers to the soils, but both lime and fertilizers are expensive. A good alternative to overcome soil acidity is the use of aluminum (Al)‐tolerant cultivars. The objective of this study was to test the hypothesis, by use of a pot experiment, that sorghum cultivars tolerant to Al toxicity are able to use P from phosphate rock more efficiently than are susceptible cultivars. Three sorghum (Sorghum bicolor L. Moench) cultivars, Chaguaramas III (Ch), AI‐tolerant, Decalb D59 (D59), and Pioneer 8225 (Pi), both Al‐susceptible, were grown in the greenhouse for 20 and 35 days in two acid soils fertilized with 0 and 100 mg P kg‐1 as triple superphosphate (SP) and Riecito phosphate rock (PR). Santa Maria soil was very low in available P (2 mg kg‐1) and highly saturated in Al saturation (64.5%) and Pao soil was higher in available P (20 mg kg‐1) and low in Al saturation (6.5%). Chaguaramas dry matter production, P uptake and root length was higher in Santa Maria soil as compared with Pi and D59 when grown with both SP and PR fertilization. Chaguaramas response to PR in Pao soil was not as good as in Santa Maria soil. The results of our experiment suggest that Al‐tolerant Ch is able to utilize P from PR more efficiently in soils like Santa Maria than Al‐susceptible cultivare Pi and D59.  相似文献   

19.
Abstract

Knowledge of the distribution of soil organic matter (SOM) fractions is important in managing soils toward a sustainable agricultural system in a tropical environment. However, data on Histosols is limited. This study developed 19 profiles of Histosols and soils with high organic-matter content from different regions of Brazil. Soil organic matter was fractionated into fulvic acids (FAF), humic acids (HAF), and humin (HUM). The ratios HAF/FAF and AE (alkaline extract)/HUM were calculated. The objectives were to evaluate the method for SOM fractionating in Histosols and related soils and to correlate the distribution of organic fractions with other soil attributes. The humic fractions presented significant correlations with other soil attributes, the best being the correlation between FAF and nutrient level. The HAF and HUM presented high correlation with cationic exchange capacity, active acidity (H+) and pH. Humin and the alkaline extract absorbance measured at 380 nm and 465 nm and presented good correlation with total organic carbon.  相似文献   

20.
Two Ca‐efficient and 3 Ca‐inefficient tomato lines selected on the basis of dry matter production, Ca concentrations in tissues, and severity of Ca deficiency symptoms were grown in nutrient solutions containing 6 levels of total Ca ranging from 15 to 365 mg in 70 mg increments. All lines responded to increased Ca supply by increasing in dry weight and by accumulating Ca. The critical Ca concentrations in the shoots were 0.25% and 0.40% on a dry weight basis for the efficient and inefficient lines, respectively. Concentrations of Ca, K, Mg, P, and NO3 ‐ were lower in shoots and except for Mg were lower in roots of efficient plants than in the inefficient plants. For all lines as more Ca was available in the media and as Ca increased in the shoots and roots, the concentrations of the nutrients other than Ca declined. The declines in concentrations of K and Mg were not due to dilution by higher dry matter production in the efficient lines relative to the inefficient ones, although the total accumulation of Ca, P, and NO3 ‐ did not vary with Ca supplied. Antagonism among cations may account for differences in efficiency among lines of tomato.  相似文献   

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