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本研究以羔羊为研究对象,利用外源激素刺激其卵巢发育,收集卵母细胞,筛选后进行成熟培养,研究获得的卵母细胞和成熟后的数量和质量。分别探讨了不同周龄、不同激素来源和使用剂量、重复超排和季节对辽宁绒山羊超数排卵的影响。结果表明,5～6周龄为辽宁绒山羊羔羊的适宜超排时期;国产FSH+PMSG组超排反应最好,加拿大产FSH+PMSG组均获得体外成熟的卵母细胞数量最多;春季是适于进行卵母细胞采集的季节,而夏季不适合进行卵母细胞采集;重复超排获卵数较初次超排极显著降低,但体外成熟率优于首次超排。 相似文献
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《中国畜牧兽医文摘》2012,(3)
<正>绒山羊母羔卵母细胞在体外成熟过程中,成熟培养液中添加12mg/ml的进口牛血清白蛋白(BSA)可以替代血清培养液,即含10%胎牛血清(FBS)的培养液,并不影响卵母细胞最终成熟。绒山羊母羔卵母细胞体外成熟后的核成熟率可达到55%左右,但其胞质成熟的质量与成年羊的卵母细胞相比有较大差距。 相似文献
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选择4~8、8~12、12~16周龄羔羊进行超排处理,研究羔羊卵巢与卵泡的发育情况、卵母细胞活体采集效果及其体外发育能力。结果表明:羔羊超排处理后卵巢大小随着周龄的增加明显下降;4~8周龄组可用卵泡数、回收卵母细胞数极显著高于8~12周龄组和12~16周龄组(P0.01),但卵母细胞的回收率随着周龄的增加而升高;12~16周龄的A、B级卵母细胞所占比率较高,A、B级与C级卵母细胞的成熟率差异极显著(P0.01);对成熟卵母细胞进行体外受精,C级卵母细胞卵裂率显著低于A级与B级卵母细胞(P0.05);酉州乌羊幼羔体外胚胎移植妊娠率为27.03%,生产羔羊14只。结果提示,4~8周龄酉州乌羊羔羊超排效果优于8~12、12~16周龄段羔羊,活体采集的羔羊卵母细胞可经体外成熟、体外受精和胚胎移植获得后代。 相似文献
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铁对牛卵母细胞体外成熟和体外受精的影响 总被引：1，自引：0，他引：1
本试验研究铁对体外生产牛胚胎的影响。从屠宰场收集牛卵巢,抽取卵巢表面的卵母细胞,采用体外成熟和体外受精的方法,研究不同浓度的铁(0.45mg/L,0.81mg/L,1.96mg/L,2.78mg/L)对牛卵母细胞体外成熟和体外受精的影响。结果如下:当卵母细胞在体外培养22h时,不同浓度的铁之间对卵母细胞体外成熟影响较小,差异不显著(p>0.05),且与对照组之间差异不显著(p>0.05);在体外受精的研究中,铁浓度为1.96mg/L和2.78mg/L的受精卵培养液可以明显提高8细胞胚胎发育率,差异显著(p<0.05);铁浓度为1.96mg/L的受精卵培养液可以明显提高囊胚发育率,囊胚发育率为31.6%,与其他浓度的铁相比,差异显著(p<0.05)。本试验结果说明:在卵母细胞体外成熟阶段,培养液中的铁对卵母细胞体外成熟没有影响,但对受精后早期胚胎的发育有促进作用,1.96mg/L是比较合适的早期胚胎培养液中铁的添加量。 相似文献
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以辽宁绒山羊母羔为研究对象,通过超排母羔、卵母细胞体外成熟、体外受精、胚胎移植技术等试验研究,建立了绒山羊母羔体外胚胎生产技术体系。结果表明:PMSG+FHS组与FSH+PMSG组超排方法获得的卵母细胞数无显著差异(P0.05),但PMSG+FHS组卵裂率和囊胚率均极显著高于FSH+PMSG组(P0.01)。成熟液添加EGF组PBⅠ率、卵裂率均显著高于对照组(P0.05);20 ng/m L EGF组PBⅠ率显著高于10 ng/m L组(P0.05),卵裂率差异不显著(P0.05)。卵母细胞成熟培养24~27 h组第一极体率与卵裂率均显著高于21~24 h组(P0.05);SOF受精体系卵裂率显著高于TALP(P0.05),囊胚率极显著高于TALP受精系(P0.01)。秋季胚胎移植受体母羊16只,产羔3只。 相似文献
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为了评价紫花苜蓿干草对不同类别辽宁绒山羊的营养价值,研究不同类别绒山羊对各种营养素消化利用能力,开展了本次试验研究。选择成年母羊、育成母羊、成年种公羊、育成公羊和羔羊各4只,依次设为1、2、3、4、5组,只饲喂苜蓿干草。测定不同类别辽宁绒山羊对苜蓿草各营养物质的消化、代谢和利用能力,评价苜蓿草对辽宁绒山羊的营养价值。结果表明,成年母羊对苜蓿草的纤维类和脂肪营养的消化率均高于育成公母羊,而育成公母羊对苜蓿草能量的表观代谢率和利用率均高于成年公母羊。羔羊对苜蓿草能量利用率均低于成年公母羊和育成公、母羊,并与育成公羊差异极显著（P〈0．01）;对苜蓿草钙、磷、纤维类营养、粗蛋白和粗脂肪的表观消化率也低于其他类绒山羊。 相似文献
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《中国草食动物科学》2015,(4)
以4~8周龄的辽宁绒山羊母羔为研究对象,研究不同年龄阶段羔羊卵泡发育数量和发育质量的变化,同时还比较了不同基础液作为受精液对体外受精和早期卵裂的影响,并尝试研究了在早期胚胎的发育培养液中添加EDTA的效果。结果表明:6~8周龄母羔的卵母细胞体外成熟率和受精率均显著高于4~6周龄母羔;利用SOF液作为受精体系的基础液时体外受精的效果更好,卵裂率达42.47%;在早期胚胎的发育液中添加10μmol/L EDTA可有效提高16细胞以上的胚胎数。 相似文献
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以我国地方品种小尾寒羊作为供体,对不同年龄羔羊(6～8周龄和12～14周龄)超数排卵效果以及卵母细胞冷冻保存对体外受精、体外胚胎发育、胚胎移植产羔的影响进行研究。结果表明:6～8周龄组羔羊只均超排处理获卵数和可用卵数(60.8枚和58.2枚)显著高于12～14周龄组(27.3枚和26.0枚)(P<0.05);经玻璃化冷冻—解冻后的卵母细胞体外受精,冷冻组的卵裂率和桑椹胚发育率(67.8%和35.6%)均显著降低于对照组(79.2%和53.8E)(P<0.05);玻璃化冷冻保存体外生产的胚胎,经移植后成功产下4只健康羔羊(4/52)。 相似文献
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探讨品种对羔羊超数排卵效果和卵母细胞发育能力的影响,并将体外受精胚胎进行了移植,为研究利用绵羊羔羊卵母细胞生产后代提供理论和技术方法。对4～8周龄不同品种羔羊用FSH+PSMG处理后获得的平均卵母细胞数量和体外胚胎卵裂率和囊胚率进行了比较,并比较成熟液中有无β-巯基乙醇对胚胎卵裂率和囊胚率的影响。结果:各品种获得的平均卵母细胞数分别为:哈萨克为210.22枚,湖羊为0枚,美利奴为135.80枚,湖羊与哈萨克的杂交后代为37.80枚,湖羊羔羊和湖羊与哈萨克羊的杂交羔羊获得的平均卵母细胞数显著低于哈萨克羔羊(P<0.01,P<0.05)。哈萨克羔羊卵裂率(69.0%)显著高于湖羊与哈萨克羊杂交羔羊(66.4%,P<0.05);哈萨克羔羊囊胚率(17.5%)和美利奴羔羊囊胚率(16.2%)显著高于湖羊×哈萨克羔羊(13.8%)(P<0.01,P<0.05)。β-巯基乙醇对羔羊胚胎卵裂率影响不显著,对囊胚率影响极显著(P<0.01)。哈萨克羔羊卵子体外受精获得的2-8细胞胚胎进行输卵管移植,14只受体羊中7只妊娠,妊娠率为50.0%;共产羔9只,其中8只存活。 相似文献
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Maturation Status, Protein Synthesis and Developmental Competence of Oocytes Derived from Lambs and Ewes 总被引：2，自引：0，他引：2
HPS Kochhar B Wu LHA Morris BC Buckrell JW Pollard PK Basrur & WA King 《Reproduction in domestic animals》2002,37(1):19-25
The efficacy of oocyte selection for in vitro embryo production depends on the abundance and diameter of follicles, cumulus layers around the oocytes and subsequent fertilization. Application of `ovum pick-up' technique allows us to utilize partially matured oocytes for embryo production even from juvenile subjects. To compare their developmental competence, oocytes derived from lambs and ewes and cultured in maturation medium for up to 26 h were assessed at 2 h intervals by confocal microscopy after chromatin and microtubulin-specific fluorochrome labelling. Lamb oocytes reached second meiotic metaphase (MII) at lower numbers at 24 h (60.0%) and 26 h (28.6%) whereas 85.7% of adult-derived oocytes attained MII status by 24 h of maturation. Radiolabelling of oocyte proteins revealed higher incorporation of [35 S-]-methionine and [35 S]-cysteine in adult-derived oocytes compared to lamb oocytes. Although the cleavage rate of lamb oocytes was similar to that of ewe oocytes, the proportion reaching blastocyst stage was significantly lower (p < 0.05) in the lamb-derived oocytes. However, blastocysts from both types of oocytes displayed similar cell lineage allocations to inner cell mass and trophectoderm. 相似文献
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Burkhard MJ Garry F 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2004,33(4):244-248
Background: A 35-day-old male lamb with Mycoplasma ovis infection (previously Eperyihrozoon avis ) was evaluated because of severe hypoglycemia (serum glucose 4 mg/dL, Hitachi 704 automated chemistry analyzer) inconsistent with the animal's condition. Whole blood glucose concentration measured with a glucometer was 74 mg/dL. Objective: The purpose of this study was to investigate this discrepancy through in vitro evaluation of the patient's blood.
Methods: Blood was incubated alone, with increasing concentrations of plasma, or with equine serum of known glucose concentration for 0,15, 30, and 60 minutes at room temperature; end-point glucose concentrations were compared with blood from a control sheep handled similarly.
Results: A rapid decline in glucose concentration was observed in heparinized or EDTA anticoagulated whole blood from the infected lamb incubated alone or with the equine serum. Glucose concentrations in incubated samples from a control sheep remained stable. Incubation of increasing concentrations of heparinized blood with autologous plasma resulted in decreased glucose concentrations in patient, but not control, blood. As parasitemia decreased after treatment, serum glucose concentration increased, serum lactate concentration decreased, and in vitro glucose concentration stabilized.
Conclusions: These findings are consistent with parasite-associated in vitro glucose consumption. An increase in the lamb's plasma glucose concentration associated with reduction of parasite load suggested excess glucose consumption also may have occurred in vivo. 相似文献
Methods: Blood was incubated alone, with increasing concentrations of plasma, or with equine serum of known glucose concentration for 0,15, 30, and 60 minutes at room temperature; end-point glucose concentrations were compared with blood from a control sheep handled similarly.
Results: A rapid decline in glucose concentration was observed in heparinized or EDTA anticoagulated whole blood from the infected lamb incubated alone or with the equine serum. Glucose concentrations in incubated samples from a control sheep remained stable. Incubation of increasing concentrations of heparinized blood with autologous plasma resulted in decreased glucose concentrations in patient, but not control, blood. As parasitemia decreased after treatment, serum glucose concentration increased, serum lactate concentration decreased, and in vitro glucose concentration stabilized.
Conclusions: These findings are consistent with parasite-associated in vitro glucose consumption. An increase in the lamb's plasma glucose concentration associated with reduction of parasite load suggested excess glucose consumption also may have occurred in vivo. 相似文献
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本研究旨在比较1、3月龄绵羔羊激素处理后卵巢、子宫及血液中促卵泡激素(FSH)变化,研究不同月龄对羔羊卵泡发育的影响。通过对1、3月龄羔羊进行FSH和孕马血清促性腺激素(PMSG)处理,比较羔羊在激素处理前后血液中FSH水平和卵巢、子宫大小变化,卵巢上2～8 mm卵泡的数量和卵母细胞体外成熟、受精后胚胎的发育情况。结果表明:1月龄羔羊实验组体内整体FSH水平高于3月龄羔羊(P<0.05)。1月龄羔羊注射外源激素后两侧卵巢可获卵母细胞数(42.3±2.5、36.8±1.1)枚及体外受精囊胚发育率(16.33%±0.96%)显著高于3月龄羔羊卵母细胞数(10.0±0.7、8.5±0.6)枚及囊胚率(9.29%±1.55%)(P<0.05)。羔羊进行超数排卵处理时卵巢上卵泡发育与血液中FSH水平密切相关,且较高的FSH水平预示着较多的卵泡发育。 相似文献