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1.
Hai lan CuiXiangju Li Guiqi WangJianping Wang Shouhui WeiHongyu Cao 《Pesticide biochemistry and physiology》2012,102(3):229-232
The increasing use of AHAS-inhibiting herbicides has resulted in evolved resistance in key dicot weeds infesting cereal cropping systems worldwide. Shepherd’s purse (Capsellabursa-pastoris) is a common dicot weed species in wheat in China with populations that have evolved resistance to the AHAS herbicide tribenuron-methyl. The seeds of eight resistant populations were collected from wheat fields and one susceptible population from road side in Hebei province of China. All eight populations showed high level resistance to tribenuron-methyl with resistance indices of over 100 fold based on whole plant dose response assays in the greenhouse. Comparison of the AHAS gene sequences of the susceptible and resistant populations with Arabidopsis revealed that proline at position 197 of the AHAS gene was substituted by threonine in population CAPBU-HB-2, serine in populations CAPBU-HB-3, CAPBU-HB-4, CAPBU-HB-5, and CAPBU-HB-6, leucine in population CAPBU-HB-7 and CAPBU-HB-8, histidine in population CAPBU-HB-9. The study confirmed tribenuron-methyl resistance in shepherd’s purse in Hebei province of China due to target site mutations at AHAS codon position 197. 相似文献
2.
麦田猪殃殃对苯磺隆抗药性的快速检测 总被引：1，自引：0，他引：1
以采自山东、河南、安徽、陕西等地麦田及非农田的10个猪殃殃Galium aparine生物型为试材,运用单剂量甄别技术对不同生物型对苯磺隆的抗药性进行了检测,并以温室盆栽法及皿内抗性水平测定法验证其可靠性和可行性。结果表明:在甄别剂量(有效成分)85 mg/L下,猪殃殃不同生物型萌发率存在显著性差异,可以较好区分猪殃殃抗、感生物型;温室盆栽法及皿内抗性水平测定法结果一致,除采自山东泰安和陕西周至的麦田猪殃殃对苯磺隆仍处于敏感状态外,其他3个地区麦田猪殃殃均产生了不同程度的抗药性,且均与皿内单剂量甄别法结果一致,表明皿内单剂量甄别法具有可靠性和可行性。 相似文献
3.
荠菜对乙酰乳酸合成酶抑制剂类除草剂的抗性水平及其分子机制 总被引：1，自引：1，他引：0
为明确荠菜种群对苯磺隆的抗性水平及其靶标抗性产生的分子机制,采用整株水平测定法测定了荠菜对苯磺隆及其他5种乙酰乳酸合成酶（ALS）抑制剂类除草剂的抗性水平,同时扩增和比对了荠菜抗性和敏感种群之间ALS基因的差异。结果显示:与敏感种群15-ZMD-1相比,抗性种群15-ZMD-5对苯磺隆产生了高水平抗性,抗性倍数为219.6;15-ZMD-5种群不同单株中共存在3种突变方式,分别为ALS基因197位点脯氨酸（CCT）突变为亮氨酸（CTT）、574位点色氨酸（TGG）突变为亮氨酸（TTG）以及单株同时发生上述197和574位点的氨基酸突变。15-ZMD-5抗苯磺隆种群对嘧草硫醚、啶磺草胺和氟唑磺隆均产生了高水平的交互抗性,抗性倍数分别为41.2、79.3和87.8;对双氟磺草胺和咪唑乙烟酸产生了低水平的交互抗性,抗性倍数分别为8.5和5.6。分析表明,荠菜抗性种群ALS基因发生的氨基酸突变可能是导致其对ALS抑制剂类除草剂产生抗性的重要原因之一。 相似文献
4.
为明确河南省部分地区的多花黑麦草Lolium multiflorum种群对乙酰辅酶A羧化酶（acetylCoA carboxylase,ACCase）和乙酰乳酸合成酶（acetolactate synthase,ALS）抑制剂类除草剂的抗性水平和抗性机理,采用整株生物测定法测定采自新乡市和驻马店市的多花黑麦草种群对ACCase抑制剂类除草剂精噁唑禾草灵、炔草酯、唑啉草酯和ALS抑制剂类除草剂甲基二磺隆、氟唑磺隆、啶磺草胺的抗性水平,并对多花黑麦草ACCase和ALS靶标酶编码基因进行克隆及氨基酸序列比对,分析其靶标抗性机理。结果显示,与多花黑麦草敏感种群HNXX01相比,HNZMD04和HNXX05种群对6种除草剂均产生了抗性,HNZMD04种群对精噁唑禾草灵和啶磺草胺的相对抗性倍数分别为44.65和40.31,对炔草酯和氟唑磺隆的相对抗性倍数分别为11.91和11.93;HNXX05种群对精噁唑禾草灵和氟唑磺隆的相对抗性倍数分别为27.70和25.67。HNZMD04和HNXX05抗性种群的ACCase基因均发生了D2078G突变,2个种群的突变率分别为55%和70%;HNZMD04... 相似文献
5.
A Papaver rhoeas population resistant to several acetolactate synthase (ALS) inhibiting herbicides, called 25/98, was found in Catalonia (Northeastern of Spain). This population has an altered form of the enzyme that showed cross-resistance to several herbicides of this group. The highest resistance was found with tribenuron-methyl and sulfometuron-methyl. Studies were conducted to define the molecular basis of this resistance. Two regions of the ALS gene were amplified using degenerated universal primers and sequenced. Population 25/98 contained a single nucleotide substitution in domain A changing Pro197 by Ser (using the nomenclature of Arabidopsis thaliana) that confers sulfonylurea resistance. Another change was detected in a region located outside of any conserved domains described to date, but its implication in the resistance remains unclear. We analyze the putative role of the found mutations in relation to the observed resistance using a putative three-dimensional model of the Papaver ALS enzyme. 相似文献
6.
河南省部分地区麦田荠菜对苯磺隆的抗性水平及抗性靶标分子机制 总被引：1，自引：0，他引：1
为明确河南省部分地区麦田荠菜Capsella bursa-pastoris对苯磺隆的抗性水平及抗性靶标分子机制,采用整株生物法测定了12个荠菜种群的抗性水平,并对乙酰乳酸合成酶(acetolactate synthase,ALS)离体活性和ALS基因突变进行了测定分析。结果表明,商丘市民权县花园村(MQ)、周口市西华县小于楼村(XH)、平顶山市叶县穆寨村(YX)、许昌市长葛市董庄村(CG)采集的荠菜种群对苯磺隆产生了较高的抗性,GR_(50)分别为129.14、110.67、62.91和85.29 g/hm~2,抗性倍数分别为215.23、184.45、104.85和142.15倍;ALS离体活性测定所得I_(50)分别为5.85、4.87、1.38和3.83μmol/L,抗性倍数分别为83.57、69.57、19.71和54.71倍;其余8个种群的GR_(50)在0.60~2.86 g/hm~2之间,抗性倍数在1.00~4.77之间;I_(50)在0.07~0.37μmol/L之间,抗性倍数在1.00~5.29之间。荠菜种群MQ、XH的ALS基因Domain A区域第197位脯氨酸(CCT)均突变为丝氨酸(TCT),荠菜种群CG的第197位脯氨酸(CCT)突变为亮氨酸(CTT),表明靶标ALS基因突变是荠菜对苯磺隆产生抗性的重要原因之一,但荠菜种群YX的ALS基因保守区内暂未发现突变位点,其抗药性可能由其它原因造成。 相似文献
7.
Monitoring of beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) resistance to chlorantraniliprole in China 总被引：1，自引：0，他引：1
Beet armyworm, Spodoptera exigua is a major insect pest of vegetables in China, and has been reported to develop resistance to many broad-spectrum insecticides. Recently registered chlorantraniliprole provides a novel option for control of this pest resistant to other conventional insecticides. The susceptibilities of field collected populations were measured by diet incorporation assay with neonate, obvious variation of susceptibility was observed among the 18 field populations with LC50 values varying from 0.039 to 0.240 mg/liter. Moderate resistant level was discovered in 8 of 18 field populations, other 8 populations had become low level tolerance to chlorantraniliprole, and only one population in all the field colonies remained susceptible. Biochemical assays were performed to determine the potential mechanisms involved in tolerance variation. Field populations displayed varied detoxification enzyme activities, but the regression analysis between chlorantraniliprole toxicities and enzyme activities demonstrated each field population might have specific biochemical mechanisms for tolerance. Artificial selection in laboratory with chlorantraniliprole was carried out, 23 generations of continuous selections resulted in 11.8-fold increase in resistance to chlorantraniliprole, and 3.0-fold and 3.7-fold increases in mixed function oxidase and esterase, respectively. Compared with the susceptible strain kept in laboratory the selection strain had developed 128.6-fold resistance to this insecticide. Synergism assays showed the detoxification enzymes might not involved in the resistance observed in field collected populations and the selected strain. 相似文献
8.
Jamshid Ashigh Peter J. Smith François J. Tardif 《Pesticide biochemistry and physiology》2009,95(1):38-46
A population of Amaranthus powellii that had survived imazethapyr did not have any of the documented AHAS mutations causing resistance in this species in Ontario. The goal of this research was to confirm resistance in this population, determine its molecular basis and develop diagnostic tests. Whole plant dose-response experiments showed the resistant population had greater than 25-fold resistance to the selective agent imazethapyr at the whole plant level. Whole plant resistance to other herbicides ranged between 9-fold to flumetsulam and 85-fold to flucarbazone. This was confirmed at the enzyme level with resistance ranging between 25-fold to imazethapyr and 485-fold to thifensulfuron. AHAS from the resistant population had 16% higher specific activity than that of the susceptible population and it was also less sensitive to feedback inhibition by valine, leucine and isoleucine. Genomic DNA was extracted to PCR amplify and sequence the AHAS gene and to determine the applicability of diagnostic tests. Sequencing of the AHAS gene revealed a single point mutation of thymine1125 to guanine coding for an Asp376Glu substitution. Two different diagnostic tests, restriction fragment length polymorphism and PCR amplification of specific allele, were able to correctly identify the resistant population from the susceptible control and from other resistant populations. Although this mutation appears to confer higher resistance to pyrithiobac and flucarbazone, two herbicides not registered in Eastern Canada, it endows plants with enough protection to allow survival to imazethapyr, the main selective agent in Ontario. 相似文献
9.
Tao JinJunliang Liu Zhibo HuanCuixia Wu Yaling BiJinxin Wang 《Pesticide biochemistry and physiology》2011,100(2):160-164
Capsella bursa-pastoris, a winter annual weed in the mustard family, can not be controlled by tribenuron after the herbicide has been continuously used for several years. The resistant biotype Lz-R was the generation of a population collected from Liangzhu, a place where tribenuron had been used for more than 15 consecutive years. To confirm and characterize the resistance of C. bursa-pastoris to tribenuron, whole-plant bioassays were conducted in the greenhouse. The results of whole-plant bioassays revealed that Lz-R was highly resistant to tribenuron with the resistance index (GR50 Lz-R)/(GR50 Lz-S) up to 236.6. To investigate the molecular basis of resistance in C. bursa-pastoris, the acetolactate synthase (ALS) genes were sequenced and compared between susceptible and resistant biotypes. Analysis of the nucleotide and deduced amino acid sequences between the biotypes indicated that one substitution had occurred in Domain A, cytosine by thymine (CCT to TCT) at position 197, that led to a change of the amino acid proline in the susceptible to serine in the Lz-R. 相似文献
10.
河南省麦田荠菜对苯磺隆的抗性及其交互抗性 总被引：1，自引：0，他引：1
为明确河南省荠菜Capsella bursa-pastoris种群对苯磺隆的抗性水平及其可能存在的抗性机理,应用整株法测定了采自驻马店及南阳等6个荠菜发生严重市的10个荠菜种群对苯磺隆的抗性,扩增和比对了荠菜苯磺隆抗性种群及敏感种群之间靶标酶乙酰乳酸合成酶基因ALS的差异,并使用单剂量法测定了以上种群对双氟磺草胺、啶磺草胺及氟唑磺隆等ALS抑制剂类除草剂的交互抗性。结果表明,驻马店市的汝南县冯湾村(ZMD-1)及平舆县五里路村(ZMD-3)荠菜种群对苯磺隆的抗性倍数分别为3.1和2.5,表现出低水平抗性;驻马店市汝南县赖楼村(ZMD-2)和周口市川汇区文庄村(ZK-1)荠菜种群对苯磺隆的抗性倍数分别为21.7和57.8,表现出高水平抗性;南阳市唐河县上屯村(NY-2)荠菜种群对苯磺隆的抗性倍数为116.5,表现出极高水平抗性,其它种群对苯磺隆仍然较敏感。NY-2、ZMD-2和ZK-1种群的ALS基因第197位氨基酸由脯氨酸(CCT)分别突变为丝氨酸(TCT)、丙氨酸(GCT)和亮氨酸(CTT),其它种群中均未发现有突变产生;这3个种群在氟唑磺隆推荐剂量处理下,死亡率仅为18.9%、23.3%和11.1%,说明已对氟唑磺隆产生了较高水平的交互抗性,其中NY-2种群对双氟磺草胺和啶磺草胺产生了低水平交互抗性,推荐剂量下死亡率分别为82.2%和83.1%。表明ALS基因突变很可能是导致荠菜种群对苯磺隆等ALS抑制剂类除草剂产生抗性的重要原因。 相似文献
11.
Fipronil resistance mechanisms were studied between the laboratory susceptible strain and the selective field population of rice stem borer, Chilo suppressalis Walker in the laboratory. The borer population was collected from Wenzhou county, Zhejiang province. After five generations of selection, fipronil resistance ratio was 45.3-fold compared to the susceptible strain. Synergism experiments showed that the synergistic ratios of PBO, TPP and DEF on fipronil in susceptible and resistant strains of C. suppressalis were 7.55-, 1.93- and 2.91-fold, respectively, and DEM showed no obvious synergistic action on fipronil. Activities of carboxylesterase and microsomal-O-demethylase in the resistant strain were 1.89- and 1.36-fold higher that in susceptible strain, and no significant difference of glutathione-S-transferase activity was found between the resistant and susceptible strains. The Km and Vmax experiments also demonstrated that fipronil resistance of C. suppressalis was closely relative to the enhanced activities of carboxylesterase and microsomal-O-demethylase. Moreover, cross-resistance between fipronil and other conventional insecticides and the multiple resistant properties of the original Wenzhou’s population were also discussed. 相似文献
12.
山东省部分市县麦田杂草麦家公Lithospermum arvense对苯磺隆的抗药性 总被引：1，自引：0，他引：1
采用温室盆栽法和培养皿法测定了山东省部分市县冬小麦田杂草麦家公Lithospermum arvense L.对苯磺隆的抗药性水平,以及其抗药性生物型乙酰乳酸合成酶(ALS)对苯磺隆的敏感性。温室盆栽结果显示,供试杂草对苯磺隆产生了不同程度的抗药性,其中胶州麦家公生物型抗性水平最高,抗性倍数为12.8倍;培养皿法测定结果也显示胶州麦家公生物型抗性水平最高,但抗性倍数为3.89倍。交互抗性测定结果表明,胶州抗性麦家公生物型对其他ALS抑制剂噻吩磺隆和苄嘧磺隆已产生不同程度的交互抗性,其中对噻吩磺隆的抗性倍数达到3.11倍。离体条件下,与敏感生物型ALS活力的抑制中浓度(IC50)相比较,胶州抗性麦家公生物型的IC50值是敏感麦家公的 2.65倍。表明ALS敏感性降低可能是山东部分市县麦家公对苯磺隆产生抗药性的重要原因之一。 相似文献
13.
猪殃殃对AHAS抑制剂靶标抗性的快速分子检测 总被引：2，自引：0，他引：2
为建立猪殃殃靶标抗性快速检测方法,并明确小麦田猪殃殃Galium aparine var.tenerum对AHAS抑制剂靶标的突变类型及分布,从河南、陕西、安徽、江苏和山东5省不同田块采集疑似对AHAS抑制剂产生抗性的猪殃殃植株,采用特异性引物PCR扩增靶标酶AHAS基因保守区片段,并以直接测序法检测采集样品,通过与拟南芥AHAS基因序列比对分析后明确其突变位点。结果显示,在5省25个农田的样品中共有19个农田检测到AHAS突变,分布在河南、安徽和江苏3省;在检测样品中发现突变发生在2个位点,共有3种突变类型,分别是197位脯氨酸(CCC)突变为丙氨酸(GCC)或丝氨酸(TCC),或者是574位色氨酸(TGG)突变为亮氨酸(TTG),检测结果与田间药效反应基本一致。这种用特异性引物扩增目的片段测序的方法,由于其可以在生长当季进行检测,适用于田间靶标突变抗性猪殃殃的快速检测与监测。 相似文献
14.
药剂对小菜蛾抗性及敏感品系乙酰胆碱酯酶抑制作用比较 总被引：5，自引：1，他引：4
采用浸叶法测定了云南通海、元谋和澜沧的小菜蛾plutella xylostella田间种群对常用杀虫剂的抗药性。结果表明,云南上述地区小菜蛾田间种群对各类杀虫剂均产生了不同程度的抗性。对有机磷类药剂的抗药性为1.74~31.1倍;对菊酯类药剂的抗药性为7.41~764倍;对阿维菌素类药剂则产生了 5.60~4.06×104倍的抗性。通过离体和活体试验测定了药剂对小菜蛾头部乙酰胆碱酯酶(AChE)的抑制作用。敌敌畏和灭多威对通海抗性品系AChE离体和活体内的抑制中浓度(I50)分别是敏感品系的209、26.5倍和2.21、2.16倍;敌敌畏对通海小菜蛾种群的离体和活体内抑制中时间(IT50)小于敏感品系,分别是敏感品系的0.32和0.17倍;而灭多威对通海小菜蛾种群的离体和活体内抑制中时间(IT50)则大于敏感品系,分别是敏感品系的1.37和1.74倍。 相似文献
15.
A field population of the rice stem borer (Chilo suppressalis Walker) with 203.3-fold resistance to triazophos was collected. After 8-generation of continuous selection with triazophos in laboratory, resistance increased to 787.2-fold, and at the same time, the resistance to isocarbophos and methamidophos was also enhanced by 1.9- and 1.4-fold, respectively, implying some cross-resistance between triazophos and these two organophosphate insecticides. Resistance to abamectin was slightly enhanced by triazophos selection, and fipronil and methomyl decreased. Synergism experiments in vivo with TPP, PBO, and DEM were performed to gain a potential indication of roles of detoxicating enzymes in triazophos resistance. The synergism results revealed that TPP (SR, 1.92) and PBO (SR 1.63) had significant synergistic effects on triazophos in resistant rice borers. While DEM (SR 0.83) showed no effects. Assays of enzyme activity in vitro demonstrated that the resistant strain had higher activity of esterase and microsomal O-demethylase than the susceptible strain (1.20- and 1.30-fold, respectively). For glutathione S-transferase activity, no difference was found between the resistant and the susceptible strain when DCNB was used as substrate. However, 1.28-fold higher activity was observed in the resistant strain when CDNB was used. These results showed that esterase and microsomal-O-demethylase play some roles in the resistance. Some iso-enzyme of glutathione S-transferase may involve in the resistance to other insecticides, for this resistant strain was selected from a field population with multiple resistance background. Acetylcholinesterase as the triazophos target was also compared. The results revealed significant differences between the resistant and susceptible strain. The Vmax and Km of the enzyme in resistant strain was only 32 and 65% that in the susceptible strain, respectively. Inhibition tests in vitro showed that I50 of triazophos on AChE of the resistant strain was 2.52-fold higher. Therefore, insensitive AChE may also involved in triazophos resistance mechanism of rice stem borer. 相似文献
16.
小偃6号成株期高温抗条锈性遗传分析 总被引：5，自引：4，他引：1
为揭示小偃6号抗病机制和培育持久抗病品种,采用常规杂交分析方法,在小麦抽穗期利用小麦条锈菌小种CYR30、CYR32和Su11-4对小偃6号、铭贤169及其杂交F1、F2、F2∶3接种,平均气温达到21℃时对小偃6号进行了抗条锈性调查和遗传分析。结果显示,接种CYR30、CYR32时,F1代表现高感,F2代群体中抗感分离比例符合1 R∶15 S的理论比例。接种Su11-4时,F1代表现高抗,F2代群体中抗感分离比例符合3R∶1S的理论比例。研究表明小偃6号对CYR30、CYR32的抗病性均由2对隐性基因累加作用控制,对Su11-4的抗病性由1对显性基因控制。 相似文献
17.
The present paper aims to quantify the degree of resistance development in Rotala indica Koehne var. uliginosa Koehne to sulfonylurea (SU) herbicides based on whole plant responses. Seeds of resistant (from Omagari City, Akita Prefecture, Japan) and susceptible (from Tsukuba City, Ibaraki Prefecture, Japan) R. indica plants were seeded in 200 cm2 pots. The plants, at the 1.5 leaf stage, were blanket-applied with different rates of three sulfonylurea herbicides: bensulfuron methyl (BSM), pyrazosulfuron ethyl (PSE) and imazosulfuron (ISN). Of the three herbicides, the weed appears to have developed the highest degree of resistance to BSM. The resistant/susceptible (R/S) values based on mortality, root length and dry weight are consistently highest at 197, 157 and 101, respectively. Responses based on shoot length, however, showed a higher R/S value for PSE. Roots of R. indica are more sensitive to higher rates of SU herbicides than the shoots. Indeed, a high degree of resistance to SU herbicides have evolved in R. indica , for which the recommended field rates are not enough for effective control of the weed. 相似文献
18.
Xuguo Zhou Gautam Sarath Laurence D. Chandler 《Pesticide biochemistry and physiology》2004,78(2):114-125
Increased hydrolytic metabolism of organophosphate insecticides has been associated with resistance among Nebraska western corn rootworm populations. In this study, resistance-associated esterases were partially purified by differential centrifugation, ion exchange, and hydroxyapatite column chromatography, with a final purification factor of 100-fold and recovery of approximately 10%. Kinetic analysis of the partially purified enzyme indicated that the Km of the group II esterases was identical for the two populations, although Vmax was consistently threefold higher in the resistant population. A putative esterase, DvvII, was further purified to homogeneity by preparative polyacrylamide gel electrophoresis. DvvII is a monomer with a molecular weight of approximately 66 kDa, although three distinct isoforms with similar pIs were evident based on isoelectric focusing gel electrophoresis. Immunoassays with the Myzus persicae E4 antiserum indicated that group II esterases from D. v. virgifera were cross-reactive and expressed at much higher titers in the resistant population relative to the susceptible counterpart. These results suggest that the resistance is likely associated with overproduction of an esterase isozyme in resistant D. v. virgifera populations. 相似文献
19.
中国北方部分地区麦田荠菜对苯磺隆的抗性水平 总被引：9，自引：2，他引：7
为明确北方地区冬小麦田荠菜对苯磺隆的抗性水平,运用培养皿法和温室盆栽法分别测定了山东、山西、河南、河北、陕西5省13个地区采集点麦田潜在抗药性生物型对苯磺隆的抗性水平,并分别测定了驻马店梁祝镇采集点荠菜潜在抗药性生物型和敏感生物型乙酰乳酸合成酶(ALS)对苯磺隆的敏感性。培养皿法测定结果表明:驻马店梁祝镇采集点荠菜抗药性生物型对苯磺隆的抗性水平最高,抗性倍数为6.17倍,其他采集点荠菜抗性倍数在0.94～2.04倍之间,仍处于较为敏感状态。温室盆栽法测定结果表明:驻马店梁祝镇采集点荠菜抗性倍数仍为最高,达到233倍, 其他地区采集点荠菜抗性倍数在1.23～3.73倍之间,尚未产生明显的抗药性。离体条件下,苯磺隆对荠菜抗药性和敏感生物型ALS的抑制中浓度(IC50)分别为0.664 μmol/L和0.053 3 μmol/L,抗药性生物型的抗性倍数达12.5倍。结果表明,驻马店梁祝镇采集点荠菜已对苯磺隆产生了较高水平的抗药性,而其体内ALS敏感性降低可能是抗药性产生的原因之一。 相似文献
20.
双唑草酮为中国创制的新型对羟基苯基丙酮酸双氧化酶(HPPD)抑制剂类除草剂，已于2018年取得中国农药登记。为了测定其对麦田抗性杂草荠菜的除草效果，共采集了36个荠菜种群，其中1个敏感种群采自未施用过除草剂的路边，其他35个疑似抗性种群均采自于中国苯磺隆使用历史超过20年的冬小麦田。在温室中采用整株盆栽法测定了荠菜种群对双唑草酮、苯磺隆、双氟磺草胺、甲基二磺隆、唑草酮和2甲4氯的抗性水平。结果表明，有28个种群对苯磺隆产生了抗性，其中种群K16009、K17005、15053和17003表现出高水平抗性。这4个种群对其他除草剂的交互抗性检测试验表明:种群K16009、K17005和15053对双氟磺草胺和甲基二磺隆表现出高水平交互抗性，其抗性指数范围为25-321，其GR50值远高于供试药剂登记的田间推荐剂量；而上述4个抗性种群对双唑草酮、唑草酮和2甲4氯均较为敏感，其GR50值均低于供试药剂登记的田间推荐剂量。唑草酮和2甲4氯也可用于小麦田抗性荠菜种群的防治，但该类药剂对施药时间要求较为严格。本研究结果表明，双唑草酮可有效防除对乙酰羟基酸合成酶（AHAS）类除草剂已经产生抗性的荠菜。 相似文献