共查询到20条相似文献，搜索用时 31 毫秒
1.
High-resolution chromosome sorting and DNA spot-blot analysis assign McArdle's syndrome to chromosome 11 总被引：23，自引：0，他引：23
R V Lebo F Gorin R J Fletterick F T Kao M C Cheung B D Bruce Y W Kan 《Science (New York, N.Y.)》1984,225(4657):57-59
A rapid gene-mapping system uses a high-resolution, dual-laser sorter to identify genes from separate human chromosomes prepared with a new stain combination. This system was used to sort 21 unique chromosome types onto nitrocellulose filter papers. Several labeled gene probes hybridized to the sorted chromosomal DNA types predicted by their previous chromosome assignments. The skeletal muscle glycogen phosphorylase gene was then mapped to a portion of chromosome 11 by spot blotting normal and translocated chromosomes. 相似文献
2.
The role of the c-mos gene in the 8;21 translocation in human acute myeloblastic leukemia 总被引：2，自引：0，他引：2
M O Diaz M M Le Beau J D Rowley H A Drabkin D Patterson 《Science (New York, N.Y.)》1985,229(4715):767-769
The human c-mos proto-oncogene is located on chromosome 8 at band q22, close to the breakpoint in the t(8;21) (q22;q22) chromosome rearrangement. This translocation is associated with acute myeloblastic leukemia, subgroup M2. The c-myc gene, another proto-oncogene, has been mapped to 8q24. The breakpoint at 8q22 separates these genes, as determined by in situ hybridization of c-mos and c-myc probes. The c-mos gene remains on the 8q-chromosome and the c-myc gene is translocated to the 21q+ chromosome. Southern blot analysis of DNA from bone marrow cells of four patients with this translocation showed no rearrangement of c-mos. 相似文献
3.
Hu-ets-1 and Hu-ets-2 genes are transposed in acute leukemias with (4;11) and (8;21) translocations 总被引：32，自引：0，他引：32
N Sacchi D K Watson A H Guerts van Kessel A Hagemeijer J Kersey H D Drabkin D Patterson T S Papas 《Science (New York, N.Y.)》1986,231(4736):379-382
Human probes identifying the cellular homologs of the v-ets gene, Hu-ets-1 and Hu-ets-2, and two panels of rodent-human cell hybrids were used to study specific translocations occurring in acute leukemias. The human ets-1 gene was found to translocate from chromosome 11 to 4 in the t(4;11)(q21;23), a translocation characteristic of a subtype of leukemia that represents the expansion of a myeloid/lymphoid precursor cell. Similarly, the human ets-2 gene was found to translocate from chromosome 21 to chromosome 8 in the t(8;21)(q22;q22), a nonrandom translocation commonly found in patients with acute myeloid leukemia with morphology M2 (AML-M2). Both translocations are associated with expression different from the expression in normal lymphoid cells of ets genes, raising the possibility that these genes play a role in the pathogenesis of these leukemias. 相似文献
4.
T B Shows A Y Sakaguchi S L Naylor D V Goedell R M Lawn 《Science (New York, N.Y.)》1982,218(4570):373-374
At least ten leukocyte interferon genes and the single known fibroblast interferon gene have been localized on the pter leads to q12 region of human chromosome 9. Gene mapping was accomplished by blot hybridization of cloned interferon complementary DNA to DNA from human-mouse cell hybrids with a translocation involving human chromosome 9. Supporting evidence suggests these genes are clustered. 相似文献
5.
中性/碱性转化酶(Alkaline or neutral invertase,N/A-lnv)是木薯淀粉合成过程中的一种关键酶。笔者以木薯华南6号古铜期嫩叶为材料制备染色体标本,利用荧光原位杂交和原位PCR技术对N/A-lnv基因家族的11个成员进行了物理定位。结果表明,基因MeNINV5,MeNINV9和MeNINV10均位于第4号染色体上,其中基因MeNINV9和MeNINV10位于短臂上,到信号点的百分距离分别为68.52和95.35,基因MeNINV5位于长臂上,到着丝粒的百分距离为22.71;基因MeNINV4和nINV1均位于第6号染色体长臂上,到着丝粒的百分距离分别为43.16和80.71;基因MeNINV6和MeNINV7分别位于第7号和第17号染色体的长臂上,信号点到着丝粒的百分距离分别是15.38和57.97;基因MeNINV1,MeNIN,V2,MeNINV3和MeNINV8分别位于第11号、第9号、第5号和第16号染色体的短臂上,信号位点到着丝粒的百分距离分别是40.10,51.88,91.75和76.33。中性/碱性转化酶基因家族内部部分成员之间存在连锁关系。研究结果可为木薯淀粉的高效积累机制及木薯种质遗传改良提供分子细胞遗传学依据。 相似文献
6.
Detection of bcr-abl fusion in chronic myelogeneous leukemia by in situ hybridization 总被引：13，自引：0，他引：13
D C Tkachuk C A Westbrook M Andreeff T A Donlon M L Cleary K Suryanarayan M Homge A Redner J Gray D Pinkel 《Science (New York, N.Y.)》1990,250(4980):559-562
Chronic myelogeneous leukemia (CML) is genetically characterized by fusion of the bcr and abl genes on chromosomes 22 and 9, respectively. In most cases, the fusion involves a reciprocal translocation t(9;22)(q34;q11), which produces the cytogenetically distinctive Philadelphia chromosome (Ph1). Fusion can be detected by Southern (DNA) analysis or by in vitro amplification of the messenger RNA from the fusion gene with polymerase chain reaction (PCR). These techniques are sensitive but cannot be applied to single cells. Two-color fluorescence in situ hybridization (FISH) was used with probes from portions of the bcr and abl genes to detect the bcr-abl fusion in individual blood and bone marrow cells from six patients. The fusion event was detected in all samples analyzed, of which three were cytogenetically Ph1-negative. One of the Ph1-negative samples was also PCR-negative. This approach is fast and sensitive, and provides potential for determining the frequency of the abnormality in different cell lineages. 相似文献
7.
The human interleukin-2 receptor is an inducible growth factor receptor present on the surface of activated T lymphocytes. The receptor is required for a normal T-cell immune response. High-resolution fluorescence-activated chromosome sorting and DNA spot-blot analysis with complementary DNA's for the interleukin-2 receptor indicated that the receptor gene was located on chromosome 9, 10, 11, or 12. In situ hybridization studies showed that the interleukin-2 receptor gene is on the short arm of chromosome 10, p14----15. 相似文献
8.
Locus of the alpha-chain of the T-cell receptor is split by chromosome translocation in T-cell leukemias 总被引：23，自引：0，他引：23
J Erikson D L Williams J Finan P C Nowell C M Croce 《Science (New York, N.Y.)》1985,229(4715):784-786
Mouse lymphoma cells were hybridized with two human acute T-cell leukemias with a t(11;14) (p13;q11) translocation and the segregated hybrids were examined for the presence of the DNA segments coding for the constant (C) and the variable (V) regions of the alpha chain (C alpha and V alpha) of the T-cell receptor. The C alpha segment was translocated to the involved chromosome 11 (11p+) while the V alpha segment remained on the involved chromosome 14 (14q-). The data indicate that the locus for the alpha chain of the T-cell receptor is split by the chromosomal breakpoint between the V alpha and the C alpha gene segments, and that the V alpha segments are proximal to the C alpha segment within chromosome band 14q11.2. 相似文献
9.
A major human histone gene cluster on the long arm of chromosome 1 总被引：13，自引：0，他引：13
L Green R Van Antwerpen J Stein G Stein P Tripputi B Emanuel J Selden C Croce 《Science (New York, N.Y.)》1984,226(4676):838-840
A human histone gene cluster was assigned to chromosome 1 by Southern blot analysis of DNA's from a series of mouse-human somatic cell hybrids with 32P-labeled cloned human H4 and H3 histone DNA as probes. Localization of this histone gene cluster on the long arm of chromosome 1 was confirmed by in situ hybridization of this DNA probe to metaphase chromosomes. 相似文献
10.
Molecular analysis of a constitutional X-autosome translocation in a female with muscular dystrophy 总被引：19，自引：0，他引：19
S E Bodrug P N Ray I L Gonzalez R D Schmickel J E Sylvester R G Worton 《Science (New York, N.Y.)》1987,237(4822):1620-1624
The gene responsible for Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) maps to the X chromosome short arm, band Xp21. In a few females with DMD or BMD, the Xp21 region is disrupted by an X-autosome translocation. Accumulating evidence suggests that the exchange has physically disrupted the DMD/BMD locus to cause the disease. One affected female with a t(X;21)(p21;p12) translocation was studied in detail. The exchange points from both translocation chromosomes were cloned, restriction-mapped, and sequenced. The translocation is reciprocal, but not conservative. A small amount of DNA is missing from the translocated chromosomes; 71 to 72 base pairs from the X chromosome and 16 to 23 base pairs from the 28S ribosomal gene on chromosome 21. 相似文献
11.
Involvement of the bcl-2 gene in human follicular lymphoma 总被引：69，自引：0，他引：69
12.
Interferon-beta-related DNA is dispersed in the human genome 总被引：6，自引：0，他引：6
A D Sagar P B Sehgal L T May M Inouye D L Slate L Shulman F H Ruddle 《Science (New York, N.Y.)》1984,223(4642):1312-1315
Interferon-beta 1 (IFN-beta 1) complementary DNA was used as a hybridization probe to isolate human genomic DNA clones lambda B3 and lambda B4 from a human genomic DNA library. Blot-hybridization procedures and partial nucleotide sequencing revealed that lambda B3 is related to IFN-beta 1 (and more distantly to IFN-alpha 1). Analyses of DNA obtained from a panel of human-rodent somatic cell hybrids that were probed with DNA derived from lambda B3 showed that lambda B3 is on human chromosome 2. Similar experiments indicated that lambda B4 is not on human chromosomes 2, 5, or 9. The finding that DNA related to the IFN-beta 1 gene (and IFN-alpha 1 gene) is dispersed in the human genome raises new questions about the origins of the interferon genes. 相似文献
13.
Zhang Xueyong Phillip M Banks P.J. Larkin 《中国农业科学》2000,(1):56-62
Z1, Z2, Z3, Z4, Z5 and Z6 are alien addition lines to wheat involving Thinopyrum intermedium chromosomes. We have characterized the Thinopyrum intermedium chromosomes or segments in these lines using multi-color florescence in situ hybridization. The probes used included total genomic DNA of Pseudoroegneria stipfolia (St) and cloned probes of highly tandem repetitive DNA pSc119. 2 and pAs1. Disomic addition lines Z1, Z2 and Z6 have the same single pair of alien chromo-somes carrying the resistant gene(s) to barley yellow dwarf virus (BYDV). This alien chromosome is a St/E translocation; within the long arm, there is a big insertion of an E-genome chromosomalsegment (30%). Disomic addition line Z3 carries one pair of St/E Robertsonian translocation chromosomes ; on the short arm (E) there is a nuclear organizer region, which expresses in some cells. In Z5, the added chromosome is one pair of translocated chromosomes. Chromosomes 2D, 3D and 3Stwere involved in the translocation with great possibility〔2IS · 3DL (0. 47) - 3StL (0. 53)〕. The St segment is responsible for resistance to leaf and stem rusts. Addition line Z4 also carries the translo cated chromosome found in Z5, but in addition carries one pair of 7AS (0. 64) - 7StS (0. 36) · 7StL translocation chromosomes. The 7St fragment bears the stripe rust resistance, and replaces the normal 7A. All of the translocations in Z1, Z2, Z6 and Z3 existed in one of their parents, the wheat Th. intermedium partial amphiploid, Zhong 5. The two wheat-Th. intermedium translocations in Z4 and Z5 occurred during the backcrossing of Zhong 5 to the other wheat varieties in the development of the addition lines. Spontaneous homoeologous translocations showed a close genome relationship between wheat and Th. intermedium. This paper also demonstrated the potential of highly repetitive sequences DNA in verification and characterization of translocation chromosomes. 相似文献
14.
Gene for human insulin receptor: localization to site on chromosome 19 involved in pre-B-cell leukemia 总被引：15，自引：0，他引：15
Consistent chromosomal translocations in neoplastic cells may alter the expression of proto-oncogenes that are located near the breakpoints. The complementary DNA sequence of the human insulin receptor is similar to those of the EGF receptor (erbB oncogene) and products of the src family of oncogenes. With in situ hybridization and Southern blot analysis of somatic cell hybrid DNA, the human insulin receptor gene was mapped to the distal short arm of chromosome 19 (bands p13.2----p13.3), a site involved in a nonrandom translocation in pre-B-cell acute leukemia. 相似文献
15.
Duchenne muscular dystrophy involving translocation of the dmd gene next to ribosomal RNA genes 总被引：11，自引：0，他引：11
R G Worton C Duff J E Sylvester R D Schmickel H F Willard 《Science (New York, N.Y.)》1984,224(4656):1447-1449
Duchenne muscular dystrophy (DMD) is a severe X-linked disorder leading to early death of affected males. Females with the disease are rare, but seven are known to be affected because of a chromosomal rearrangement involving a site at or near the dmd gene on the X chromosome. One of the seven has a translocation between the X and chromosome 21. The translocation-derived chromosomes from this patient have been isolated, and the translocation is shown to have split the block of genes encoding ribosomal RNA on the short arm of chromosome 21. Thus ribosomal RNA gene probes may be used to identify a junction fragment from the translocation site, allowing access to cloned segments of the X at or near the dmd gene and presenting a new approach to the study of this disease. 相似文献
16.
Mapping of the human Blym-1 transforming gene activated in Burkitt lymphomas to chromosome 1 总被引：7，自引：0，他引：7
C C Morton R Taub A Diamond M A Lane G M Cooper P Leder 《Science (New York, N.Y.)》1984,223(4632):173-175
Blym-1, a transforming gene detected by transfection of NIH 3T3 cells with DNA from Burkitt lymphomas, was mapped to the short arm of chromosome 1 (1p32) by chromosomal in situ hybridization. The Blym-1 gene was not physically linked to the cellular myc oncogene or to any of the immunoglobulin gene loci implicated in the characteristic chromosomal translocations in Burkitt lymphoma. 相似文献
17.
水稻完全显性早熟性的发现和基因定位 总被引：9，自引：1，他引：9
研究发现早籼核不育系6442S-7具有完全显性早熟特性，它与13个不同类型的中、迟熟品种杂交，F1抽穗期与6442S-7完全相同或十分相近，对F2和B1F1遗传分析表明该早熟性主要受2对显性基因控制，利用F2群体和4种分子标记技术，将6442S-7携带的1个显性早熟基因定位于水稻第3染色体短臂造近端问一侧，该基因与RAPD标记OPI11.557、SSR标记RM22、RM231、RFLP标记C515和AFＬＰ标记ＰＴ６７１的遗传距离分别为１.5、３.0、６.7、１０.9和１２.4cM，该基因系首次发现并定位，暂命名为Ｅf-cd(t).6442S-7作为完全显性早熟性种质资源，对水稻遗传改良具有重要的应用价值。 相似文献
18.
【目的】探究异染色质纽在玉米、类玉米及其杂种后代染色体上分布的特点及遗传稳定性。【方法】利用组成玉米异染色质纽的180-bp重复序列和TR-1元件对二倍体多年生类玉米（Zea diploperennis, DP）、玉米自交系330及其远缘杂交后代异源种质纯系540的有丝分裂中期染色体进行荧光原位杂交,观察杂交信号在3个材料染色体上的位置、强弱及分布数量。【结果】玉米自交系330的第2、3、5、6、7号染色体长臂的近末端区显示较强杂交信号。DP的第2、3、5、6、7、8、9号染色体的长臂末端检测出异染色质纽,其中2号染色体在短臂的末端也检测到异染色质纽杂交信号。杂交后代540的第2、5、7染色体长臂近末端区检测到较强的杂交信号;3个材料的第6染色体短臂末端的随体上均显示强杂交信号。【结论】玉米大部分异染色质纽成分不能稳定遗传,其表现出来的多态性可以作为鉴定玉米和类玉米杂种后代的细胞学标记。 相似文献
19.
玉米C型Cms育性恢复基因Rf4的SSR标记 总被引：20，自引：0，他引：20
对A619与Cms237F2及BC1群体的育性观察结果表明，恢复系A619有一对恢复基因，用微卫星标记（SSR）将A619的恢复基因定位在玉米第8染色体短臂上，与SSR引物bnlg2307连锁，距bnlg2307 12.3cM。 相似文献