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槟榔黄化病是世界各地槟榔生产上普遍发生的毁灭性病害。至今没有根治的有效方法,根本原因是其病原还没有明确鉴定。有报道表明槟榔黄化病的病原可能是植原体。本文利用巢式PCR方法,对海南3个市县采集的28个黄化病植株的不同器官进行了植原体检测,结果表明在2市县9个槟榔黄化病病株叶片中检测到了植原体,而在根和茎中没有发现植原体。同源性聚类发现其属于翠菊黄花组B亚组。 相似文献
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茅苍术种植基地内发现少数植株表现为扁茎、叶序紊乱、花变叶等症状,与植原体侵染引发的症状类似。为确定是否是植原体侵染,以及是何种植原体侵染,本研究利用植原体通用引物P1/P7和R16F2n/R16R2对扁茎变异茅苍术植原体的16S rDNA基因序列进行巢式PCR扩增、克隆、测序,并进行分析。结果表明,所得扩增产物约1.2 kb的植原体特异片段,通过对实验结果分析,确定该苍术变异原因为植原体感染。系统进化分析结果表明,引起茅苍术发生扁茎变异的植原体属于翠菊黄化组(Aster yellows group)16SrI-B亚组。本研究首次从分子水平确定了引起中国湖北英山地区茅苍术扁茎变异的病原菌为植原体,明确了其分类地位,为指导苍术该病害流行学研究和苍术植原体防治提供了理论依据。 相似文献
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<正>(接上期)10.苹果黄叶病综合防控技术研究及应用单位名称:河北工程大学评价日期:2010.11.25评价单位名称:邯郸市科技局针对石灰性土壤上生长的果树普遍存在的缺铁问题,采用邻二氮杂菲铁示踪的方法,研究了铁在树体内吸收、运输和 相似文献
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对缺铁黄叶病的主要症状进行了介绍,并对该病害的发病原因进行了相关分析,针对发病原因提出对应的防治措施,希望能为我国桃树种植起到一定的帮助作用。 相似文献
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枣疯病植原体的快速检测 总被引:2,自引:1,他引:1
以表现丛枝症状的‘金丝4号’枣树为试材,以叶片总DNA粗提物为模板通过PCR扩增技术克隆16S rDNA基因,建立了枣疯病植原体快速检测方法。序列分析结果显示,枣疯病植原体‘金丝4号’株系(JWB-Jinsi4,TA)与JWB-G1(AB052876)同源性为99.7%,归属于16Sr Ⅴ-B组。实验结果表明,以DNA粗提物为模板的PCR扩增技术,可快速有效地检测枣疯病植原体,为生产实践中枣疯病的诊断和防治提供技术支持。 相似文献
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茄镰孢霉在欧美杨溃疡病发生中的作用 总被引:1,自引:1,他引:0
欧美杨溃疡病是国内广泛栽种的欧美杨(Populus×euramericana)品种上新发现的病害,其中分离到的Lonsdalea quercina和Fusarium solani在室内外接种,均可在欧美杨上产生症状,但L. quercina引起的症状更为典型,F. solani在致病过程中所起的作用尚不明确。为了明确欧美杨溃疡病发生过程中2种微生物的关系,通过拮抗试验、接种试验和野外调查,并将所得数据用SPSS软件分析。结果表明,L. quercina和F. solani两者间不具有拮抗关系,L. quercina和F. solani混合接种与L. quercina、F. solani单独接种时所形成病斑的相关系数分别在0.9和0.8以上,L. quercina与病害发生相关性更高,起主要致病作用;F. solani在溃疡病发生中后期出现较多,致病力较低,常常伴随L. quercina发生,扮演着加重病害发生的角色。 相似文献
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为了分析茶饼病病叶表面微生物多样性,分离鉴定病叶上真菌微生物。以茶饼病病叶与健康叶片为试材,采用扫描电镜对叶表面进行观察,利用16S和ITS测序分析不同叶片的细菌与真菌群落组成。在茶饼病病害叶片表面检测到113种细菌,252种真菌;健康叶片上检测到237种细菌,161种真菌。病叶细菌的多样性显著低于健康叶片,病叶真菌的多样性高于健康叶片。与健康叶片相比,枝孢属和拟盘多毛孢属真菌在病叶中丰度较低,被孢霉属丰度较高。采用PDA培养基从感病叶片表面分离鉴定获得了枝状枝孢菌,该菌可能在茶饼病的感染过程中发挥着重要作用。茶饼病感染后茶叶表面微生物群落结构发生显著变化,分离鉴定出的枝状枝孢菌可能与茶饼病的致病过程密切相关。 相似文献
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Wang Jing Zhai Weibo Meng Jing Gao Huan Shi Jinyan Zhang Wenwei Han Rong Qi Fangjun 《棉花学报》2018,30(6):486-491
[Objective] Cotton Alternaria leaf spot disease causes serious damage during cotton production. The aim of this study was the optimization of an inoculation method for cotton Alternaria leaf spot. [Method] An Alternaria alternata spore suspension was used as the inoculum. Three inoculation methods were tested and compared: smearing with spores plus retaining moisture using a sealed plastic bag; smearing with spores plus retaining moisture by intermittently spraying with water; and spraying spores plus retaining moisture by intermittently spraying with water. [Result] Inoculated cotton leaves were most readily infected by smearing with spores plus intermittently spraying with water. This inoculation method was effective for distinguishing resistant cotton cultivars from susceptible ones, but often caused nonuniform disease lesions on cotton leaves; thus, the incidence of diseased leaves was random and somewhat nonuniform. Spraying spores plus retaining moisture by intermittently spraying with water led to a relatively longer disease pathogenesis and slower disease progression; however, the incidence of diseased leaves was relatively consistent and uniform, thus providing repeatable inoculation experimental conditions compared with the other two inoculation methods. [Conclusion] Spraying spores plus retaining moisture by intermittently spraying with water is a suitable inoculation method for studying cotton resistance to Alternaria leaf spot. 相似文献
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The performance of insect-resistant transgenic poplar trees (Populusnigra) expressing a Cry1Ac gene from Bacillus thuringiensis subsp. Kurstaki HD-1 against poplar defoliators was evaluated in the field at the Manas Forest Station in Xinjiang Uygur
Autonomous Region during1994–1997. The results showed that the average percentage of highly damaged leaves on the transgenic
trees was 10% while that on the control trees in nearby plantations reached 80–90%. The average number of pupae per m2 of soil at 20cm depth in transgenic poplar plantation was 18 which was only 20% of that found in the non-transgenic control
field. The number of pupae and the leaf-damage on transgenic trees described above are all far below the threshold set for
chemical protection measures. The non-transformed poplar trees grown in the same plantation with the transgenic trees were
also protected indicating that cross protection occurred between these two kinds of trees. Insect-resistant transgenic poplar
trees have a potential application value in afforestation.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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生物有机肥对衰弱病杨梅营养改良及强壮树势的作用 总被引:1,自引:1,他引:0
衰弱病是杨梅产业上的严重病害,为开发杨梅衰弱病的关键防控技术,选择3种生物有机肥和挪威复合肥进行试验,不施肥病树为对照,研究施生物有机肥和复合肥对病树营养改良和树势的影响。结果表明,3种生物有机肥10~20 kg/株施用量防病以及营养改良效果较好,总体上优于复合肥,病害防效66.12%~82.64%,复合肥无防效,使病树土壤pH和有机质含量升高,土壤内的速效氮、有效钙、镁、有效硫、锌、铜、铁等7种元素以及叶片内的钙、硫、铁、锰、硼等5种元素含量显著增加,改善了病树营养生长和果实品质,单果重和可溶性固形物分别增加51.8%~77.0%和1.50~3.36个百分点,可滴定酸含量降低0.16~0.31个百分点。综上所述,生物有机肥对杨梅衰弱病有一定防效,可改善病树土壤和叶片营养元素含量,促进营养生长,提升果实品质。 相似文献
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摘要:以患病牙鲆的囊肿组织为实验材料,冰裕匀浆后采用低速离心的方法提取淋巴囊肿病毒,用粗提的病毒液感染牙鲆鳃细胞系FG-9307,同时应用以抗淋巴囊肿病毒单克隆抗体为第一抗体的间接免疫荧光抗体技术检测淋巴囊肿病毒是否感染了牙鲆鳃细胞并在其中增殖。结果发现生长旺盛的单层牙鲆鳃细胞接种淋巴囊肿病毒2天后出现明显的细胞病变效应,形成空斑,应用间接免疫荧光抗体方法检测结果为抗淋巴囊肿病毒单抗与未接种淋巴囊肿病毒的牙鲆鳃细胞呈阴性反应,而接种病毒的牙鲆鳃细胞在接种后,细胞质中有明显的特异性荧光,该结果说明牙鲆鳃细胞系是淋巴囊肿病毒的敏感细胞系。 相似文献
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基于形态学与ITS序列对冠突散囊菌多样性研究 总被引:1,自引:1,他引:0
为研究茯砖茶中冠突散囊菌多样性的问题,以来自中国3个省份的5种茯砖茶为样品,从中分离出优势微生物金花菌4、16、B1、B3、B7,依托形态学与和分子生物学技术对菌株进行鉴定。结果发现,这5株菌株均属冠突散囊菌,并且存在着遗传多样性,其中16号菌株的电镜观察结果与其他4株菌株差异明显,且菌株子囊孢子的“赤道”周围存在较多小孔;在ITS序列构建系统发育树中4、B1、B3、B7聚类为一组,16号聚类成另一组,说明冠突散囊菌可能存在亚种,这与形态学结果一致。 相似文献