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1.
2020年9月西南大学动物医学院动物医院收到重庆市荣昌区某乌鸡养殖户乌鸡病料一份(1只),送本实验室进行病理学诊断。病鸡经临床诊断后处死,进行尸体剖检,采集有病变的组织器官用10%福尔马林固定,之后制作石蜡切片及苏木精-伊红染色,光学显微镜下观察组织病理学变化。结果表明:病鸡临床表现为精神沉郁,体型消瘦,胸肌菲薄,皮包骨头;尸体剖检病变表现为腺胃、肌胃和小肠内有数条蛔虫;肝脏、脾脏、肾脏和肠系膜表面均有大小及数量不等的白色结节;其他组织器官均有不同程度损伤。综合临床症状、病理剖检及病理组织学检查结果,初步诊断为鸡蛔虫病。 相似文献
2.
要搞好猪病剖检诊断，应熟悉猪的正常组织器官，避免病理剖检时将正常组织器官误诊作病变组织器官，熟悉猪死亡后尸体的变化，避免把某些死后变化误认为是生前的病理变化，熟悉各种觉病理变化和各种猪病的特征病变，掌握正确的剖检诊断程序及方法，以确保剖检效率和发现有诊断价值的病变，剖检结果应结合病猪发病情况和外部检查情况，才能做出剖检诊断. 相似文献
3.
该文对珠海台创园羊驼养殖基地出现的羊驼死亡病例进行病理剖检、组织病理学和血液学检查、分子生物学检测。血常规及生化检查提示羊驼出现炎症，尸体剖检和切片检查显示多器官充血和出血，通过PCR及序列测定确认该羊驼存在念珠菌性肺支原体感染。本文通过对该病例的确诊，积累了同类疫病防控的经验。 相似文献
4.
从山羊鼻内腺瘤病例中分离出嗜麦芽窄食单胞菌的报告 总被引：3，自引：1，他引：2
对临床发病的山羊病例进行尸体剖检、病理学诊断和细菌学检查,分析疫病发生的原因。根据尸体剖检和病理学检查诊断为山羊鼻内腺瘤病,并继发有严重的细菌感染。根据细菌分离培养、染色特性、生理生化特性及16S rDNA序列分析鉴定为嗜麦芽窄食单胞菌。 相似文献
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6.
尸体剖检是运用病理学和其他学科的知识，通过检查尸体的病理变化来诊断疾病的方法。剖检牛时，要先采出肠道，宜采用左侧卧位。虽然习惯上用右侧卧位，因为牛胃体积很大，占腹腔整个左侧和右侧的下部，大、小肠仅占腹腔右侧的上部。 相似文献
7.
二、鸡的尸体剖检技术鸡的尸体剖检即解剖鸡的尸体,检查各器官组织的病理变化,进行科学地分析、综合,找出死亡原因,判定疾病的性质。尸体剖检过程中,不能破坏器官组织的病理变化,不能漏检器官组织的病理变化。所以剖检时要按一定的程序和技术要 相似文献
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尸体剖检是家畜病理学的重要组成部分,是运用病理学和其他学科的知识,通过检查尸体的病理变化来诊断疾病的方法。通过尸体检查,使家畜的疾病迅速得到确诊,对疾病的及时防治以及检验生前诊断是否正确,提高诊疗工作质量都具有重要意义。 相似文献
10.
要搞好猪病剖检诊断,应熟悉猪的正常组织器官,熟悉猪死亡后尸体的变化,避免把某些死后变化误认为是生前的病理变化;熟悉各种常见病理变化和各种猪病的特征病变,掌握正确的剖检诊断程序及方法,以确保剖检效率和发现有诊断价值的病变,剖检结果应结合病猪发病情况和外部检查情况,才能做出剖检诊断。 相似文献
11.
Herich R Levkutová M Kokincáková T Reiterová K Hipíková V Levkut M 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2006,53(7):340-345
Twenty-eight BALB/c mice were infected with different strains of Encephalitozoon species (Encephalitozoon cuniculi II - mouse type, E. cuniculi III - dog type, Encephalitozoon hellem, Encephalitozoon intestinalis). Five of them were infected with E. cuniculi II (mouse type) and simultaneously immunosuppressed with dexamethasone. Clinical signs of encephalitozoonosis were not remarkable. Ascites was found in two mice of dexamethasone-treated group 14 days post-infection (p.i.). The histopathological changes were found mainly in spleen and liver in the form of lymphoepithelioid granuloma. Spores were found in faeces since day 14 p.i. and visualized by Calcoflour White M2R. After cultivation on cellular cultures (VERO E6 - monkey kidney cells, RK-13 - rabbit kidney fibroblasts), the species differentiation was performed by PCR using panmicrosporidial primers (PMP1, PMP2) and specific primers (ECUN-F, ECUN-R, V1, SI-500). The differences were recorded in the immune response of immunocompetent and immunosuppressed mice. At day 60 p.i., the titres of specific antibodies measured by indirect immunofluorescence antibody test were lower (1:4096) in dexamethasone-treated mice when compared with non-immunosuppressed animals (1:8196). The significant increases of antibody titres were recorded in particular infected groups within the experiment (P < 0.01 between day 14 p.i. and day 30 p.i., P < 0.001 between day 14 p.i. and day 60 p.i.). Experimental encephalitozoonosis in non-immunosuppressed and immunosuppressed mice provides a useful model for the study of immune response and lesions associated with these protozoans. 相似文献
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Newborn cats, pigs, and sheep (3 to 14 days old) and postweanling cats (2.5 months old) that had been inoculated with Gardner feline sarcoma virus and feline leukemia virus at 10 days of age were infected experimentally with a rabbit isolate of the mammalian protozoan parasite Encephalitozoon cuniculi. Infection occurred in all cats and in some sheep, but was questionable in pigs. Brain and kidney were the 2 major target organs in cats. The lesions were compatible with, but less severe than, those of naturally infected cats and other carnivores. Of 13 cats, E cuniculi could be detected morphologically in the kidneys of 12 cats and in the brain of 1 cat. The organisms were reisolated from 2 cats with ground tissue suspension of kidney or urine sediment. The indirect immunofluorescence antibody (IFA) titers were 1:20 to 1:1,280 at the time the animals were killed, but antibodies were not detected before inoculation. Lesions were seen in the kidneys of 2 of 4 sheep. These lesions were mild, but were compatible with those in a spontaneously affected goat. Encephalitozoon cuniculi were found morphologically in the kidney of 1 sheep with lesions. All sheep had IFA titers of 1:10 to 1:20 before inoculation, and the titers were 1:20 to 1:320 when they were killed. Vasculitis, similar to the subacute-to-chronic stage of polyarteritis nodosa, was observed in 1 of 8 pigs. The lesions were primarily present in the kidney; comparable but milder lesions were also seen in the heart and brain. Antibody was not detected before inoculation.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
13.
3只临床表现斜颈、麻痹和打滚的病兔,经病理组织学检查诊断为脑炎原虫病.取其脑组织制成乳剂,接种于兔肾细胞、Vero细胞、猫肾细胞和兔脉络丛细胞.用前3种细胞均分离出原虫.该虫体表现为多种形态,呈杆状、圆形或卵圆形;Gram染色呈阳性,Goodpasture氏染色呈红色.用20%H_2O_2处理虫体,在相差显微镜下见到虫体极丝的伸展.通过虫体的形态学、染色特征和极丝的突出实验,鉴定该虫体为脑炎原虫.兔肾细胞、Vero细胞和猫肾细胞的感染率分别为40%、40%和5%,而在兔脉络丛细胞原代培养物中未检查到虫体. 相似文献
14.
Szeredi L Pospischil A Dencsö L Mathis A Dobos-Kovács M 《Acta veterinaria Hungarica》2007,55(4):525-532
A Lippizan mare aborted a male fetus a few days before the expected foaling date without showing any clinical sings. Focal lympho-histiocytic hepatitis in the foal and multiplex focal lympho-histiocytic villitis accompanied by villus necroses and marked hypertrophy of chorionic epithelial cells in the arcades were observed. Elongated nucleated organisms were seen in groups in vacuoles or solitarily located in the cytoplasm of the chorionic epithelial cells. The organisms were in large numbers and often extracellularly in areas of villitis and villus necroses. They were Gram-positive, stained with haematoxylin and eosin (HE), periodic acid-Schiff (PAS) and Giemsa, weakly with Warthin-Starry silver stain but not with G?m?ri's methenamine-silver stain. By ultrastructural and immunohistochemical examinations, the organisms were identified as microsporidia belonging to the genus Encephalitozoon. No Encephalitozoon organisms were detected in the fetal organs. This is the first reported case of equine abortion induced by Encephalitozoon sp. in Europe. Although abortion induced by Encephalitozoon is rare, microsporidia should be considered a differential diagnosis for intracellular organisms observed in the chorionic epithelial cells of horses. 相似文献
15.
Infections with Encephalitozoon cuniculi and Leptospira interrogans, serovars grippotyphosa and ballum, in a kennel of foxhounds 总被引：2，自引：0，他引：2
J R Cole L T Sangster C R Sulzer A R Pursell H C Ellinghausen 《Journal of the American Veterinary Medical Association》1982,180(4):435-437
Leptospira interrogans serovars grippotyphosa and ballum were isolated from kidney and urine of an American Foxhound pup. The pup was from a litter of 12, all of which were unthrifty. Titers for serovar grippotyphosa in pups from the litter ranged from 200 to 6,400 and 23 of 36 adult dogs in the kennel had titers to that serovar. None of the sera was tested for antibodies to serovar ballum. Leptospires were not isolated from or observed in 2 littermates and 1 penmate, but gram-positive organisms morphologically compatible with Encephalitozoon cuniculi were detected in their brains and kidneys. 相似文献
16.
Encephalitozoon cuniculi infects a wide variety of domestic and wild mammalian species including humans. Although the infection status has been studied in laboratory and pet rabbits worldwide, there is shortage of information regarding the disease in Iran. In the present study, the occurrence of infection in brains of 117 asymptomatic rabbits from six breeding and experimental units with highest population of rabbit colonies in the country (n = 60) as well as pet rabbits of pet stores in two cities (n = 57) were examined by nested-PCR. Histological sections of brains and kidneys were also studied by light microscopy. PCR results revealed that 3.3% of laboratory rabbits (2/60) and 59.6% of pet rabbits (34/57) harboured E. cuniculi in their brains. Histopathology on the other hand showed spores of the parasite in kidney and brain of one and kidney of another pet rabbit. As encephalitozoonosis may interfere with results of experiments performed on laboratory rabbits, routine screenings for identification and culling of infected animals is recommended. Furthermore, infected companion rabbits can transmit E. cuniculi to people in close contact with them, therefore, improving public knowledge of this zoonotic infection is suggested. 相似文献
17.
Encephalitozoonosis in a parrot 总被引：2，自引：0，他引：2
K B Poonacha P D William R D Stamper 《Journal of the American Veterinary Medical Association》1985,186(7):700-702
Encephalitozoon infection was diagnosed in a double yellow-headed Amazon parrot, Amazona ochrocephala. Illness was characterized by respiratory distress, decreased appetite, diarrhea, and weight loss. This gram-positive organism was present in renal tubules, intestine, and lungs. Ultrastructural features consisted of 5 polar filament coils and a single nucleus. Organisms were contained within parasitophorous vacuoles in cytoplasm of the renal tubular epithelium. 相似文献
18.
Microsporidia in dogs and cats is primarily caused by the obligate, intracellular parasite Encephalitozoon cuniculi, which is a member of the phylum Microsporidia. The aim of the current study is the detection of this parasite in stool samples of small animals of Iran, by polymerase chain reaction. Microsporidia spp. was found in 31% (31/100) of dogs (E. cuniculi (18/100), Encephalitozoon bieneusi (8/100) and Encephalitozoon intestinalis (5/100)), and 7.5% (3/40) of the specimens obtained from cats were infected with E. bieneusi. Sequencing of PCR products confirmed these results. In conclusion, Microsporidia infection seems to be fairly common in pet animals of Iran, especially in dogs. This finding could indicate the importance of pet animals as zoonotic reservoirs of microsporidial human infections. 相似文献
19.
《Journal of Exotic Pet Medicine》2007,16(3):135-145
With rabbit patients, as in other species, analyzing blood and urine samples can be useful and informative, although interpretation of the results is sometimes challenging. This article summarizes the interpretation of laboratory results from rabbits. Hematological parameters can yield information about the red blood cell population and leukocyte response to stress and pathogens. Biochemistry evaluation can be used to investigate liver, kidney, and other organ function, and urinalysis results may yield additional information about kidney function and electrolyte imbalances. Serological tests are available for several pathogens of rabbits, including Encephalitozoon cuniculi, although the significance of positive results and antibody titers is not clear. Serum protein electrophoresis aids the understanding of protein disorders and the immune response to acute and chronic inflammation. 相似文献