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用标准粘液瘤病毒人工感染中国家兔，实验结果表明，中国家兔对粘液瘤病毒极易感，感染率和死亡率为１００％；病理变化主要表现在皮下发生粘液性肿胀，真皮浅层至肌层间有大量瘤细胞浸润，瘤细胞多呈星形，也有呈梭形或蝌蚪形，粘液染色（＋），ＰＡＳ染色（＋）。在表皮的基细胞，棘细胞和瘤细胞胞浆中可见不同时期的病毒颗粒和病毒包涵体。研究结果为诊断粘液瘤病提供了理论依据和方法。 相似文献
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试用兔肾原代细胞传代；增殖兔粘液瘤病毒，呈现典型ＣＰＥ。粘液瘤病毒ＳＧ３３株在兔肾细胞上连续传３代之后，就不产生ＣＰＥ，在鸡胚上传３代再回归兔肾原代细胞，又呈现ＣＰＥ。ＳＧ＿（３３）在鸡胚上不能产生明显痘斑。维持液ｐＨ值影响ＳＧ＿（３３）在兔肾原代细胞上形成ＣＰＥ。 相似文献
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兔粘液瘤病是由兔粘液瘤病毒引起的一种高度接触性、致死性传染病,其特征为全身皮下尤其是颜面部和天然孔周围皮下发生粘液瘤性肿胀。近年来本病有向亚洲蔓延的趋势。在首次发病地区,发病率和死亡率都在90%以上。 相似文献
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兔粘液瘤病是由兔粘液瘤病毒引起的兔的一种高度接触性、致死性传染病。该病以全身皮下,尤其颜面部和天然孔周围皮下发生粘液瘤性肿胀为主要特征。1898年,Sanarelli在乌拉圭最早发现本病,之后在欧洲、美洲及澳大利亚的许多国家相继有本病的报道,目前我国尚未见有关本病的报道。近几年实验研究证明,中国家兔对兔粘液瘤病毒的发病率和死亡率均为100％,国家规定本病为二类动物传染病,随着改革开放的进行,WTO的加入,各种种兔及兔产品原料等的引入,该病对养兔业的潜在威胁很大,有必要对此病严密监控,防患于未然。现将本病作一简单介绍,以供参考。 相似文献
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马立克氏病（ＭＤ）是由疱疹病毒所引起的鸡淋巴细胞增生性瘤疾病。病毒染早期是溶细胞性的，随后产生Ｔ－细胞淋巴瘤。这些淋瘤细胞潜伏感染病毒，但仅可检测到和量的病毒基因转录和蛋白质合成。尽管有此证据表明这些病毒的转录产物可能参与肿瘤形成。而由病毒与细胞相互作用导致细胞表型转化的准确机理还未完全弄清。在这些病毒复制产物中，具有基本亮氨酸拉链结构（ｂＺＩＰ）的ｍｅｑ蛋白质是基因复制激活物，对肿瘤形成主要作用 相似文献
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SONG Lianjie CUI Yingying ZHAO Yiping BAI Dongyi REN Xiujuan TE Rigele DUGARJAVIIN Manglai LI Bei 《中国畜牧兽医》2007,47(9):2751-2758
To study a simple and rapid separation of testis Sertoli cells in Mongolian horses and ensure their activity,the testis tissue of two-year-old Mongolian horses was mechanically isolated under a low temperature environment in this experiment,and 1-3 g of testis tissue was chopped,the free red blood cells and interstitial cells were removed by gravity precipitation method.0.1% collagenase Ⅳ and 0.25% trypsin-EDTA were used for tissue digestion, and cells were cultured in medium containing 10% fetal bovine serum.After inoculation,the purified Sertoli cells were isolated and purified by differential sticking method,and the impurity cells were removed by Tris-HCl infiltration method,and were identified by alkaline phosphatase (AKP) staining,HE staining and Real-time quantitative PCR.The results showed that most of the cells began to adhere to the wall after culture 24 h,and the shape of the cells was oval.After culture 48 h,the cytoplasm increased and the refraction became stronger.After culture 3-4 d,the cytoplasm of the cells expanded into tight junction.At this time,the cells were triangular with obvious nucleoli.After culture 6-7 d,the cells entered the stable phase.Real-time quantitative PCR results showed that the expression of GATA4 and GDNF genes were extremely significantly expressed in cultured cells (P<0.01).AKP staining supported the negative expression in Sertoli cells,indicating that the isolated cultured cells were indeed Sertoli cells.In this experiment,tissue was treated by mechanical separation and two-step enzyme digestion,and testicular support cells were obtained quickly and efficiently,the method of culturing Sertoli cells in Mongolian horses testis in vitro was successfully constructed. 相似文献
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利用噬菌体环七肽库筛选与肝癌细胞特异性结合的多肽,并对其亲和力进行生物学鉴定。以HL-7702为消减细胞,HepG2为筛选靶细胞,对噬菌体随机环七肽库进行4轮全细胞消减筛选,并随机挑取60个阳性噬菌体克隆,以ELISA法鉴定其与HepG2细胞的结合活性,并取阳性克隆进行测序分析,并合成多肽进行免疫细胞化学染色鉴定。经4轮筛选,噬菌体在靶细胞HepG2上出现明显富集;利用ELISA从随机挑选的60个噬菌体克隆中得到15个与肝癌细胞具有高结合力的阳性克隆,测序并进行序列分析比对发现氨基酸序列无同源性,经免疫细胞化学染色鉴定后,发现1条多肽序列亲和力较高,为提高抗菌肽对肿瘤细胞的靶向杀伤作用奠定基础。 相似文献
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Canine venereal tumor cells were grown in monolayer and tumor tissue fragments were maintained in vitro for 56 days in medium-199. Attachment and cell replication were evident within 17 hours and monolayer was obtained by the tenth day. Growth at first comprised mainly round cells with abundance of cytoplasm and vesicular nuclei, and a few spindle-shaped cells. Later cells became more elongated and like fibroblasts. Cell degeneration started by the 17th day and most were degenerated by the 24th day. Necrosis and depletion of cell population were prominent in tumor explants during the first 2 weeks. Distinct cell multiplication was evident by the 21st day and tumor fragments were repopulated with cells resembling the original tumor by the 56th day. Two distinct morphological cell types were seen: small cells with vesicular, round to oval nuclei and acidophilic cytoplasm; and the large cells with large hyperchromatic nuclei and acidophilic cytoplasm. 相似文献
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为研究一种简单快速分离蒙古马睾丸支持细胞并保证其活性的基本方法,本试验采集2岁蒙古马睾丸组织于低温环境下进行机械分离,将1~3 g睾丸组织剪碎,采用重力沉淀法去除游离的红细胞和间质细胞;使用0.1%胶原酶Ⅳ和0.25%胰蛋白酶+EDTA逐步进行组织消化,并用含有10%胎牛血清的培养基进行细胞培养;在接种后采用差异贴壁法分离纯化支持细胞,使用Tris-HCl低渗法去除杂质细胞,并采用碱性磷酸酶（AKP）染色、HE染色、实时荧光定量PCR方法进行鉴定。结果显示,支持细胞贴壁性能较强,培养24 h后大多数细胞开始贴壁,细胞形状呈椭圆形;培养48 h后胞质增多,折光性变强;培养3~4 d细胞胞质展开紧密连接,此时细胞呈三角形,有明显的核仁,培养6~7 d细胞生长状态进入稳定期。实时荧光定量PCR结果显示,GATA4和GDNF基因在培养细胞中极显著表达（P<0.01）,AKP染色支持细胞呈阴性表达,表明分离培养的细胞确为支持细胞。本试验使用机械分离法与两步酶消化法处理组织,可快速高效地获得睾丸支持细胞,成功构建了蒙古马睾丸支持细胞体外培养方法。 相似文献
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Energy metabolism in canine erythrocytes associated with inherited high Na+- and K+-stimulated adenosine triphosphatase [(Na,K)-ATPase] activity (HK cells) was compared with that in normal canine erythrocytes (LK cells). Activities of some of the glycolytic enzymes in the HK cells were significantly higher than those in LK cells. The concentrations of adenosine triphosphate (ATP) and glycolytic intermediates in HK cells were almost equal to those in LK cells. Glucose utilization and lactate production by HK cells in vitro and incorporation of [32P]orthophosphate or [14C]glucose into 2,3-diphosphoglycerate in HK cells were higher than in LK cells. Radioactivity of [32P]ATP in HK cells was lower than in LK cells, but increased to approximately that of LK cells when (Na,K)-ATPase of HK cells was completely blocked by ouabain. When HK cells and LK cells were incubated in the absence of glucose, the concentration of ATP in HK cells was decreased more than that of LK cells. Although ouabain reduced the rate of decrease in ATP in HK cells, the decrease in ATP in HK cells was still 2-fold that in LK cells. The half-life of HK cells was about one-half that of LK cells. The results indicated that glycolysis is greater in HK cells than in LK cells, and that the increased glycolysis in HK cells was stimulated by an increased rate of ATP breakdown for active cation transport by the (Na,K)-ATPase and by increased degradation of ATP for some other pathway, eg, glutathione synthesis. Thus, the increased demand for ATP in HK cells might result in shortening the lifespan of HK erythrocytes. 相似文献
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In Mongolian gerbil, morphological changes with age in the content of endocrine cells in the pancreatic islets were analyzed and their relation evaluated by an oral glucose tolerance test. The glucose level 2 hours after glucose administration was 125 ± 5 mg./dl in the young group and 103 ± 4 mg./dl in the old group. In the dorsal portion, B cells were mainly observed in the central area of the islets, surrounded by circular layers of A cells in the peripheral area. Between the A cells and B cells, D cells were scattered or present in layers. A few PP cells were present in the peripheral area of the islets. In the ventral portion, only a few A cells were observed in the peripheral area of the islets, and B cells were surrounded by PP cells. Secretory granules of A cells generally had an electron-dense spherical core in the limiting membrane. The halo between the limiting membrane and core in A cells was narrower than that in B cells. Secretory granules of B cells were larger than those in A cells, and the core was less electron-dense, and the halo was wider. Secretory granules of D cells were similar in size to those of A cells; the core showed low electron density, and the halo was very narrow. Granules of PP cells resembled those of A cells, but the electron density of the core was slightly lower. The gerbils showed changes in glucose tolerance, the size of the pancreatic islets, the percentage of B cells, and of A cells in the dorsal portion with age. The content of endocrine cells in Mongolian gerbils was similar to that in humans, rats, mice, and hamsters. 相似文献
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In vitro infection of fractionated chicken lymphocytes by infectious bursal disease virus 总被引：1，自引：0，他引：1
Lymphocytes from bursa of Fabricius and thymus of chickens were purified and separated into the three cell subsets--T, B, and null cells--by the techniques of nylon fiber columns and cytotoxicity tests. The in vitro susceptibility of the fractionated lymphocytes to a virulent strain of infectious bursal disease virus (IBDV) was studied by using immunofluorescence as the infection criterion. B cells were highly susceptible. By contrast, T cells and null cells were insusceptible to infection by IBDV. The relationship between the target cells for virus infection and those B cells that possessed surface immunoglobulin (SIg) was tested. B cells were further divided into SIg(M)- and SIg(G)-bearing cells by immunoadsorbent columns employing anti-immunoglobulin M(IgM) (mu-specific) or anti-IgG (gamma-specific) sera coated with Sephadex. The SIg(M)-bearing cells were highly susceptible. These results suggest strongly that SIg(M)-bearing B cells were the target cells for infection by IBDV. 相似文献
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Timo Rahko 《Acta veterinaria Scandinavica》1973,14(2):245
Mast cells and globule leucocytes in the walls of the common bile ducts of seven uninfected slaughtered cows and of five cows chronically infected with Fasciola hepatica were studied by electron microscopy. The mast cells displayed numerous small intracytoplasmic granules, the matrixes of which were either strongly electron-opaque and homogeneous or less dense with a fine-granular ultrastructure. Unattached ribosomes were abundant in the cytoplasm of the cells. Many mast cells in the walls of chronically infected bile ducts were normal-looking while the cells in the subepithelial tissues often showed striking irregularities in the ultrastructure of the granules. Globule leucocytes were most numerous in the surface epithelium and were not connected by desmosomes to the epithelial cells. The nuclei of the globule leucocytes were similar to those in the mast cells except that indentations caused by the globules were more usually seen. The globules were considerably larger than the mast cell granules but bounded by similar agranular capsules. The matrix of most globules was dense and homogeneous while the other globules showed dense reticulate or lamellate structures. The relationship between the mast cell and globule leucocyte in the liver is briefly discussed with reference to recent reports on these cells in the intestinal wall. 相似文献