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对本市范围内的23个乡镇场，85个点，550份鸡血清样本进行鸡新城疫（ND）、鸡马立克氏病（MD）、鸡痘（FP）、鸡病毒性关节炎门A）、鸡腺病毒病（CELD）、白血病（AL）、鸡传染性法氏囊病（IBD）、鸡传染性支气管炎（IB）、鸡传染性喉气管炎（IL）、鸡传染性鼻炎（IC）、禽霍乱（FC）、鸡白痢（SP）、鸡减蛋综合征任DS-76）等共间种疾病进行监测。检狈OMD、FP、VA、CELD、IBD、IB、ILT、IC、FC、SP等10种病，用琼脂扩散试验即AGP法。检测ND、EDS-76用血凝抑制试验（HI）。鸡白痢用平板凝集试验。l调查结果表明，… 相似文献
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野鸟和候鸟对各种鸡病病原体的传播起很重要的作用。作者等以SPF(无特殊病原体)鸭为模型进行了鸭类对鸡病原体敏感性的研究。给SPF鸭从自然感染部位感染11种鸡病病原体:腺病毒、脑脊髓炎(AE)、鸡痘(FP)、呼肠弧、传染性气管炎(IB)、传染性法氏囊病(IBD)、传染性喉气管炎(ILT)、马立克病(MD)、新城疫(ND)、网状内皮病(RE)及火鸡疱疹(HVT)病毒,进行致 相似文献
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应用基因芯片技术检测禽重要疫病的研究——I．4种禽病检测基因芯片靶基因的克隆及鉴定 总被引：3，自引：3，他引：0
对NDV、IBV、AIV和IBDV等病原进行了分子生物学特征分析，以RT-PCR分别扩增制备NDV、IBV、IBDV和AIV的靶基因，分别命名为ND1、ND2、ND3、ND4、ND5、IB1、IB2、IB3、IB4、AI1、A12和IBD1。并分别对制备的靶基因进行了克隆和鉴定分析，经BamHⅠ酶切、PCR和核酸序列测定鉴定分析表明，除T／WZ重组质粒外，T／ND1（F48E9），T／ND2（LaSota），T／ND3（LaSota），T／ND4（F48E9），T／ND5（LaSota），T／ND5（F48E9），T／IB1（SM），T／IB1（M41），T／IB2（SM），T／IB3（M41），T／WZ，T／AI1，T／AI2，T／IBD1等14个重组质粒，均是其病原的特异性基因。该批靶基因的成功克隆和鉴定为禽疫病检测基因芯片的构建和制备提供了确实可靠的靶基因材料，是禽类疫病基因检测芯片构建和制备的关键技术之一。 相似文献
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对NDV、IBV、AIV和IBDV等病原进行了分子生物学特征分析,以RT-PCR分别扩增制备NDV、IBV、IBDV和AIV的靶基因,分别命名为ND1、ND2、ND3、ND4、ND5、IB1、IB2、IB3、IB4、AI1、AI2和IBD1.并分别对制备的靶基因进行了克隆和鉴定分析,经BamHⅠ酶切、PCR和核酸序列测定鉴定分析表明,除T/WZ重组质粒外,T/ND1(F48E9),T/ND2(LaSota),T/ND3(LaSota),T/ND4(F48E9),T/ND5(LaSota),T/ND5(F48E9),T/IB1(SM),T/IB1(M41),T/IB2(SM),T/IB3(M41),T/WZ,T/AI1,T/AI2,T/IBD1等14个重组质粒,均是其病原的特异性基因.该批靶基因的成功克隆和鉴定为禽疫病检测基因芯片的构建和制备提供了确实可靠的靶基因材料,是禽类疫病基因检测芯片构建和制备的关键技术之一. 相似文献
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鸡的传染病有多种多样,其中以新城疫(ND)、传染性支气管炎(IB)及传染性喉气管炎(ILT)的症状最危急,传染性强,造成的经济损失也很大。这些病都是由病毒引起的、以呼吸器官症状为特征的急性传染病,由于毒力强和发病时危害大,日本家畜传染病预防法规定,ND 为法定传染病,IB 和 ILT 为上报传染病。一、发生情况和疾病梗概(一)新城疫病新城疫病是由鸡新城疫病病毒(NDV)引起的传染性极强的传染病,除可感染鸡、火 相似文献
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1发病特点1.1鸡病种类增多20世纪90年代初仅见有新城疫(ND)、马立克氏病(MD)、鸡白痢(PD)等,进入90年代中后期,除上述疫病外还出现了鸡传染性法氏囊病(IBD)、传染性支气管炎(IB)、传染性喉气管炎(ILT)、鸡传染性鼻炎(IC)、鸡慢性呼吸道病(CRD)、葡萄球菌病(SC)、鸡大肠杆菌病(AC)和病毒性关节炎(AVA)等。养殖发达地区新病种类多、混合感染疾病难以及时确诊,以致造成疫病流行,影响养殖户的信心和经营。1.2死亡率增高由于危害养鸡业的流行疾病众多,在防治上有困难,在肉鸡、蛋鸡的养殖中出现了三个时期的死亡高峰。如1～15日龄为PD… 相似文献
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在80年代鸡的常见传染病仅仅见有新城疫（ND）、马立克氏病（MD）、鸡痘（FP）、禽霍乱（FC）和鸡白痢（PD）,而现代除上述疫病外还出现了传染性法氏囊病（IBD）、传染性支气管炎（IB）、鸡减蛋综合症（EDS-76）、传染性脑脊髓炎（AC）、病毒性关节炎（AVA）、鸡传染性鼻炎（IC）、鸡大肠肝菌病（AC）、鸡慢性呼吸道病（CRD）以及葡萄球菌病（SC）, 相似文献
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鸡14种病毒病基因芯片的研究 总被引：4，自引：0，他引：4
应用PCR或RT—PCR方法分别获得鸡新城疫(ND)、禽流感(AI)、鸡传染性支气管炎(IB)、鸡传染性法氏囊病(IBD)、鸡传染性喉气管炎(ILT)、鸡马立克氏病(MD)、减蛋综合征(EDS-76)、禽网状内皮组织增生症(RE)、鸡脑脊髓炎(AE)、鸡传染性贫血症(CIA)、鸡病毒性关节炎(AVA)、包涵体肝炎(IBH)、禽白血病(AL)、鸡痘(FP)各一特异性片断，以这些片断为探针，采用接触式点样技术，制成低密度的DNA阵列芯片。自病料中提取核酸，通过生物标记技术，使样品核酸标记上生物素。当样品和预点在硝酸纤维素膜上的探针杂交后．通过显色技术，即可得出诊断结果。 相似文献
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Bivalent Newcastle disease (ND)/infectious bursal disease (IBD) and trivalent ND/IBD/infectious bronchitis (IB) inactivated oil emulsion vaccines were prepared in the laboratory and evaluated under field conditions. Broiler breeder parent chickens previously vaccinated with live vaccines were inoculated with commercial monovalent ND and experimental bivalent or trivalent oil emulsion vaccines. The commercial vaccine induced a higher initial ND haemagglutination inhibition (HI) response than the experimental vaccines but, by 34 weeks after vaccination, the mean ND HI levels were not significantly different in any of the three flocks. All three vaccines provided sufficient ND immunity to protect against the clinical disease and egg production losses. The IBD responses of both flocks vaccinated with oil emulsion vaccine were similar to each other and only slightly lower than those flocks vaccinated with monovalent IBD oil emulsion vaccine in earlier experiments. Six weeks after vaccination, sufficient immunity was transferred to protect all the progeny against IBD challenge up to 33 days of age and some of them up to 45 days of age. Thirty-four weeks after vaccination of the parents with oil emulsion vaccine, the progeny were totally immune up to 27 days of age and some of them were immune until 37 days. Application of oil emulsion vaccines in bivalent or trivalent form did not impair the responses of the chickens to the monovalent components. 相似文献
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Three similar flocks of broiler breeder parent chickens that had been given live infections bronchitis (IB) vaccines during rearing were injected at 20 weeks of age with three different oil emulsion vaccines: a commercial monovalent Newcastle disease (ND) vaccine (flock A); an experimental bivalent vaccine containing ND and infectious bursal disease (IBD) components (flock B); and an experimental trivalent vaccine containing ND, IBD and IB components (flock C). One week after vaccination 40 hens from flock A and 40 from flock C were taken to the laboratory and their egg yields individually recorded. At 37 weeks of age they were challenged by aerosol exposure to virulent IB virus. The egg production dropped significantly in the hens from flock A but not in the hens from flock C. On the farm, flock C showed a higher mean IB virus antibody titre four weeks after vaccination but titres rose in all three flocks indicating the presence of active IB virus infection. No differences in egg yields were found between the three farm flocks. 相似文献
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ZHANG Zhen-hua LI Lin ZHANG Jian-wei SHEN Jia SHI Ai-hua HUANG Feng-jun ZHENG Xiao-lan JIANG Bei-yu 《中国畜牧兽医》2016,43(5):1374-1378
1-day-old SPF chickens and commercial Jingfen chickens were vaccinated with IBD immune complex(IC) vaccine, NDV La Sota vaccine were inoculated simultaneously every one week and every two weeks.NDV La Sota immunization alone was as the control group.At the 2nd, 3rd, 4th and 5th week post inoculation, blood samples were taken and the ND HI antibody were tested.Experimental chickens were challenged with high virulent NDV at the 5th week post inoculation, the protective rate of each group was calculated.The results showed the ND HI antibody were not significant different in the combined immunization of IBD IC vaccine priming and NDV La Sota vaccine boost and NDV La Sota vaccine alone immunization (P>0.05).The results indicated that IBD IC vaccine has no immunosuppression on NDV La Sota vaccine in SPF chickens and commercial Jingfen chickens. 相似文献
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鸡新城疫、传染性支气管炎、传染性法氏囊病三联弱毒疫苗的试制 总被引：1，自引：0，他引：1
用同胚培养的鸡新城疫Lasota株和传染性支气管炎H120（或H52）株以及用鸡胚成纤维细胞增殖的传染性法氏囊弱毒株以适当比例混合为抗原,用蔗糖脱脂乳作保护剂,经真空冷冻干燥制成三联弱毒疫苗。通过三批疫苗室内外各项指标的测试,表明该三联疫苗安全性能可靠,免疫效果确实,使用方法简便。在实验室进行的物理性状检验、无茵检验、支原体检验、剩余水分检验、真空度检查均符合国家标准;用10倍大剂量接种15日龄雏鸡无不良反应;以常规量颈部皮下接种免疫后,7d产生免疫力,免疫后14d抗鸡新城疫、鸡传染性支气管炎、鸡传染性法氏囊病三株强毒攻击的保护率均为,100％。对15日龄雏鸡首免,其免疫期至少为30d,在-25℃保存期为1年。 相似文献
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O A Adewuyi O A Durojaiye D F Adene 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1989,36(1):43-48
A serological survey for IBD in market guinea fowls gave a total prevalence rate of 44.3% from five different locations in Nigeria. Guinea fowl keets were susceptible to experimental IBD infection and transmitted it to in-contact sentinel chickens. The infected guinea fowl keets showed the typical clinical-pathology and seroconversion for IBD. These results suggest that guinea fowls could play an active part in the epidemiology of IBD. 相似文献
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笔者实地调查了解了目前在尼日利亚西北地区散发流行的非典型鸡新城疫的发病情况并参与了诊断与防治工作。认为免疫不当、野毒不断侵袭、鸡群免疫力低是当地发病的基本原因。症状不典型,发病季节不明显,病鸡持续零星死亡,并且在已打过ND疫苗的鸡群仍然发病是本病在当地的流行特征。文章还反映了中尼人员合作采取的综合性的谤治措施。 相似文献
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Immunosuppressive viral diseases threaten the poultry industry by causing heavy mortality and economic loss of production, often as a result of the chickens' increased susceptibility to secondary infections and sub-optimal response to vaccinations. This paper aimed to present an up-to-date review of three specific economically important non-oncogenic immunosuppressive viral diseases of chickens, viz. chicken infectious anaemia (CIA), infectious bursal disease (IBD) and hydropericardium syndrome (HPS), with emphasis on their immunosuppressive effects. CIA and IBD causes immunosuppression in chickens and the socio-economic significance of these diseases is considerable worldwide. CIA occurs following transovarian transmission of chicken anaemia virus and has potential for inducing immunosuppression alone or in combination with other infectious agents, and is characterized by generalized lymphoid atrophy, increased mortality and severe anemia. The virus replicates in erythroid and lymphoid progenitor cells, causing inapparent, sub-clinical infections that lead to depletion of these cells with consequent immunosuppressive effects. The IBD virus replicates extensively in IgM(+) cells of the bursa and chickens may die during the acute phase of the disease, although IBD virus-induced mortality is highly variable and depends, among other factors, upon the virulence of the virus strain. The sub-clinical form is more common than clinical IBD because of regular vaccination on breeding farms. Infection at an early age significantly compromises the humoral and local immune responses of chickens because of the direct effect of B cells or their precursors. HPS is a recently emerged immunosuppressive disease of 3-6-weeked broilers, characterized by sudden onset, high mortality, typical hydropericardium and enlarged mottled and friable livers, with intranuclear inclusion bodies in the hepatocytes. The agent, fowl adenovirus-4, causes immunosuppression by damaging lymphoid tissues; the presence of IBD and CIA viruses may predispose for HPS or HPS may predispose for other viral infections. Synergism with CIA or other virus infections or prior immunosuppression is necessary to produce IBH-HPS in chickens and the susceptibility of chickens infected with fowl adenovirus varies throughout the course of CIA infection. The mechanism of immunosuppression has been studied in detail for certain chicken viruses at molecular levels, which will provides new opportunities to control these diseases by vaccination. 相似文献
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The characteristics of the pathogenic infectious bursal disease virus (IBDV) that infected avian species other than commercial chickens were largely unknown. In this study, by using in vivo and molecular methods, we had characterized an IBDV isolate (named 94268) isolated from an infectious bursal disease (IBD) outbreak in Malaysian village chickens--the adulterated descendant of the Southeast Asian jungle fowl (Gallus bankiva) that were commonly reared in the backyard. The 94268 isolate was grouped as the very virulent IBDV (vvIBDV) strain because it caused severe lesions and a high mortality rate in village chickens (>88%) and experimentally infected specific-pathogen-free chickens (>66%). In addition, it possessed all of the vvIBDV molecular markers in its VP2 gene. Phylogenetic analysis using distance, maximum parsimony, and maximum likelihood methods revealed that 94268 was monophyletic with other vvIBDV isolates and closely related to the Malaysian vvIBDV isolates. Given that the VP2 gene of 94268 isolate was almost identical and evolutionarily closely related to other field IBDV isolates that affected the commercial chickens, we therefore concluded that IBD infections had spread across the farm boundary. IBD infection in the village chicken may represent an important part of the IBD epidemiology because these birds could harbor the vvIBDV strain and should not be overlooked in the control and prevention of the disease. 相似文献