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1.
Neospora caninum is a worldwide distributed protozoan that may cause neuromuscular disease in dogs and reproductive failure in domestic and wild ruminants. One axis fawn (Axis axis) and four neonates from the same deer herd died at a zoo in Argentina within a four-month period. The fawn presented with dilatation of the anal sphincter at birth and incontinence, developed weakness and ataxia and died at 14 days of age. At necropsy, a mega formation of the distal large intestine was observed. Microscopically, non-suppurative encephalitis, suppurative bronchopneumonia, fibrin necrotic enteritis and degenerative changes in the liver were observed in hematoxilin and eosin-stained tissue sections, and thick-walled N. caninum-like cysts were observed in fresh brain samples. Serologic studies for N. caninum revealed an IFAT titer of 1:6400 in the fawn and 1:25, 1:400, 1:3200 and 1:6400 in the neonates. N. caninum DNA was detected in brain samples from the fawn and from one neonate by PCR, and the parasite was isolated in vitro from the fawn’ brain after passage through gerbils (Meriones unguiculatus) and gamma-interferon knock-out mice. N. caninum DNA obtained from the fawn, neonate and isolated parasites showed the same microsatellite pattern. This suggests a common infection source for both animals.The diagnosis of N. caninum infection was confirmed, suggesting its association with perinatal mortality in captive axis deer. To the best of our knowledge, this is the first report of clinical disease associated to N. caninum infection in axis deer and of isolation of the parasite from this wild ruminant species.  相似文献   

2.
Mycoplasma ovis is a hemoplasma parasite of sheep, goats, and reindeer; however, natural hemoplasma infection in white-tailed deer has not previously been reported. Subsequent to finding many coccoid, bacillary, and ring-shaped organisms, consistent with hemotropic mycoplasmas, on RBCs from a 72-day-old female white-tailed fawn, we sought to (1) identify the putative hemoplasma observed in blood from the fawn, (2) evaluate others in the herd for hemoplasma infection, and (3) identify clinicopathologic characteristics of hemoplasma-infected white-tailed deer. EDTA-anticoagulated whole blood was collected from the fawn and 8 apparently healthy does in the same herd. CBCs were performed on 7 nonclotted samples from the fawn and 6 does. DNA was extracted from all samples, followed by PCR amplification of bacterial (16S rDNA) and protozoal (18S rDNA) genes. The nearly complete 16S rDNA product from the fawn's sample was directly sequenced and compared with known sequences in the GenBank database. Samples from the fawn and 7 of 8 does were PCR-positive using hemoplasma-specific and M ovis-specific protocols. The fawn was PCR-negative for Anaplasma spp., Babesia spp., and Theileria spp. The 16S rDNA sequence from the fawn (GenBank accession number, FJ824847) was most closely related to M ovis (AF338268), having 98.5% sequence identity. The fawn had a mild nonregenerative anemia, a neutrophilic left-shift with toxic change, aspiration bronchopneumonia, and gastrointestinal disease. Hematologic values, including blood film evaluation, in infected does were unremarkable. The M ovis-like organism may have acted as either an opportunistic or primary pathogen in the fawn. The high occurrence of subclinical infections in the does suggests that white-tailed deer may act as wildlife reservoirs for M ovis.  相似文献   

3.
益生素对梅花鹿仔鹿和羔羊日粮营养物质消化率的影响   总被引:5,自引:1,他引:4  
4只5月龄梅花鹿仔鹿和3只7月龄安装永久瘤胃瘿管的小尾寒羊,先后饲喂基础日粮(定量颗粒饲料和自由采食玉米秸秆;对照组)和基础日粮添加4 g益生素(BioLac;试验组),观察添加益生素不同动物干物质采食量,日粮营养物质的消化率和秸秆在小尾寒羊瘤胃中的降解情况。结果表明,添加益生素可显著提高仔鹿和羔羊干物质的采食量,仔鹿干物质采食量由未添加益生素的441.4 g/d提高到506.0 g/d;羔羊的干物质采食量由未添加益生素的598.8 g/d提高到719.6 g/d。但试验组干物质、有机物、粗蛋白和中性洗涤纤维的消化率(仔鹿分别为:49.3%、52.1%、57.4%和49.3%;羊分别为:53.6%、56.7%、47.9%和53.0%)显著低于对照组(仔鹿分别为:56.4%、58.7%、65.0%和56.4%;羊分别为:61.0%、64.0%、58.8%和61.0%)。结果仔鹿可消化干物质的采食量在对照组(248.9 g/d)和试验组(249.1 g/d)间无显著差异,试验组羔羊可消化干物质采食量(384.9 g/d)略高于对照组(365.7 g/d)。瘤胃降解试验结果表明,秸秆干物质,有机物质和中性洗涤纤维2-48 h,在饲喂益生素的羔羊瘤胃内的降解率均略高于未添加益生素的羔羊瘤胃内的降解率,粗蛋白在饲喂试验日粮的羔羊瘤胃内的降解率在2 h时高于在饲喂对照日粮的羔羊瘤胃内的降解率,其他时间低于在饲?  相似文献   

4.
丁玉华 《野生动物》2006,27(6):30-31
苏州西山太湖麋鹿园的麋鹿一雌同时哺乳三仔的行为,仔鹿共表现出四种吮乳模式:即一仔鹿在哺乳雌鹿后侧吮乳,二仔鹿在哺乳雌鹿腹部左侧吮乳;一仔鹿在哺乳雌鹿后侧吮乳,二仔鹿在哺乳雌鹿腹部右侧吮乳;一仔鹿在哺乳雌鹿后侧吮乳,二仔鹿在哺乳雌鹿腹部左右侧各一头吮乳;三仔鹿分别立于哺乳雌鹿的后侧、左侧和胸腹下方吮乳。太湖麋鹿一雌哺乳三仔的特殊行为的表现,既取决于鹿群的生活空间和鹿群的密度,又与哺乳雌鹿摄入的饲料质量相关联,同时也与仔鹿一时饥饿而又无法寻找到自己的母亲哺乳有关。  相似文献   

5.
6.
Malignant catarrhal fever (MCF) was diagnosed in a 2-week-old Formosan sika deer. The fawn had been previously exposed to a clinically normal neonatal wildebeest calf from which alcelaphine herpesvirus-1 was isolated. Alcelaphine herpesvirus-1 was isolated from buffy coat leukocytes and nasal and ocular secretions of the fawn during the acute illness. The fawn clinically recovered after 3 weeks. Virus was not recovered from blood at this time. Dexamethasone, given 4 months after clinical recovery, resulted in reisolation of MCF virus from blood and recrudescence of clinical MCF. The deer was euthanatized. At necropsy, pathognomonic lesions of MCF, granulomatous disease, and malignant lymphoma were observed. Antibodies to bovine leukosis viral antigens were not detected in the serum. The epidemiologic and pathogenetic importance of the findings are discussed.  相似文献   

7.
提高天山马鹿繁殖率与鹿茸产量的研究   总被引:4,自引:1,他引:3  
应用现代生物科学的方法,调整野生动物在人工驯养条件下出现的生理机能紊乱,使其产生新的综合平衡。方法:采用科学繁殖技术、中草药复合添加剂及综合配套技术。结果表明:母鹿繁殖率达8842%,仔鹿成活率达9655%,产茸量提高1926%,疾病死亡率下降566%,经济效益显著,推动了我区养鹿业的发展。  相似文献   

8.
9.
羊茅黑麦草、高羊茅及一年生黑麦草苗期抗旱生理初探   总被引:1,自引:1,他引:0  
以羊茅黑麦草TF型(羊茅型)品种Hykor、羊茅黑麦草意大利黑麦草型品种Perny、一年生黑麦草(2倍体)品种Surrey、一年生黑麦草(4倍体)品种Angus、高羊茅牧草型品种Fawn为试验材料,采用单因素设计,通过盆栽自然干旱胁迫,研究各牧草的叶片叶绿素、可溶性糖、电导率和土壤含水量的动态变化.结果表明,5种植物种...  相似文献   

10.
为阐明睾酮在鹿角柄发生过程中的作用机理,本文对6头梅花鹿的生茸区骨膜和头部的非生茸区骨膜的睾酮特异结合进行了测定。所得结果为,正常、去势雄仔鹿的生茸区骨膜中都存在一定量的睾酮特异结合(分别为:正常,细胞质为29.32,细胞核为169.63;去势,细胞核为595.84。单位:fmol/mg蛋白),而非生茸区骨膜却未测到这种特异结合;雌鹿的测定结果为,除去势雌仔鹿生茸区骨膜具有一定量的睾酮特异结合(细胞核为223.21fmol/mg蛋白)外,其余生茸区和非生茸区均不存在睾酮特异结合。这些结果表明,雄仔鹿和去势雌仔鹿生茸区骨膜中可能存在着一定量的雄激素受体,这种骨膜在特定的生理条件下受睾酮的直接作用,是睾酮的靶组织。  相似文献   

11.
The Alberes population is a native bovine breed of Catalonia with an unclear origin, which historically some authors have assumed as being composed of two different colour varieties (black and fawn). Sixteen microsatellite loci were analysed, all of them included in the AIRE2066 European Concerted Action list. Overall expected and observed heterozygosities reached values of 0.649 and 0.662, respectively. Genetic differences among black and fawn varieties were not significant (FST = 0.007), and therefore the population is a single variety with a great colour gradation. On the contrary, we detected significant genetic differences among herds (FST = 0.026; p < 0.001), showing a genetic heterogeneity over short geographical distances. The number of migrants per generation among pairs of herds oscillates between 1.46 (Roig and Freixe herds) and 5.62 (Castanyers and Roig herds). Moreover, inbreeding and bottleneck situations can be rejected. The Alberes breed has been grouped within the Cantabrian trunk, closely related to the Asturiana de la Montaña and Alistana breeds, although some other breeds may also have influenced the population along its history.  相似文献   

12.
Bovine viral diarrhea virus (BVDV) infections cause substantial economic losses to the cattle industries. Persistently infected (PI) cattle are the most important reservoir for BVDV. White-tailed deer (Odocoileus virginianus) are the most abundant species of wild ruminants in the United States and contact between cattle and deer is common. If the outcome of fetal infection of white-tailed deer is similar to cattle, PI white-tailed deer may pose a threat to BVDV control programs. The objective of this study was to determine if experimental infection of pregnant white-tailed deer with BVDV would result in the birth of PI offspring. Nine female and one male white-tailed deer were captured and housed at a captive deer isolation facility. After natural mating had occurred, all does were inoculated intranasally at approximately 50 days of pregnancy with 10(6) CCID(50) each of a BVDV 1 (BJ) and BVDV 2 (PA131) strain. Although no clinical signs of BVDV infection were observed or abortions detected, only one pregnancy advanced to term. On day 167 post-inoculation, one doe delivered a live fawn and a mummified fetus. The fawn was translocated to an isolation facility to be hand-raised. The fawn was determined to be PI with BVDV 2 by serial virus isolation from serum and white blood cells, immunohistochemistry on skin biopsy, and RT-PCR. This is the first report of persistent infection of white-tailed deer with BVDV. Further research is needed to assess the impact of PI white-tailed deer on BVDV control programs in cattle.  相似文献   

13.
Objective To measure the impact of undernourishment during the rutting period on the breeding rate of Javan rusa deer and performance of fawns from birth to weaning.
Design A random controlled trial.
Animals Two groups of 37 hinds.
Procedure Group P grazed improved pasture and group M native grassland through the rutting period. Hinds were weighed at the beginning and the end of rutting, at ultrasound scan and prior to fawning. Fawning was monitored.
Results At the end of the rutting period, the mean difference in weight between the two groups was 8.5 kg and the pregnancy rate was 95% for group P and 57% for group M. Fifty births were recorded which equates to a fertility rate at 24 hours of 60% for group P and 30% for group M. Fawn mortality occurring in the first 24 hours affected 26% of all fawns. In 54% of the cases, newborn deaths were subsequent to abandonment by the hind. Average rusa fawn weight at birth was 3.6 + 0.1 kg, regardless of sex or group. Among fawns that weighed less than 3.0 kg, the mortality rate was 55%. The newborn fawn weight was related to the weight of the hind at ultrasound scanning. The weaning rate at 20 weeks was 84% for both groups. Mean weight gains for sucking fawns were 150 g per day for the first 2 months and 108 g per day from 60 to 120 days regardless of sex or group. The mean weight at 60 and 120 days was 12.7 kg and 18.5 kg, respectively. Two regression equations enable the calculation of the weight at 60 and 120 days using the fawn weight at birth and hind weight at the beginning of the rutting period.
Conclusion A 5% weight loss for the hinds resulting from undernourishment during the rutting period causes a 32% reduction in the herd fertility rate, without affecting the weight of newborn fawns, the survival rate nor growth performances in the sucking phase.  相似文献   

14.
Six seronegative pregnant reindeer (Rangifer tarandus L) were vaccinated with killed Brucella abortus strain 45/20 with added adjuvant. These were challenge exposed with B suis type 4 after 90 days; at the same time, 4 seronegative, nonvaccinated, pregnant reindeer (controls) were given the challenge inoculum. Humoral antibodies were detected in the vaccinated reindeer by postvaccination day 14. A marked increase in antibody levels also occurred after they were challenge exposed, but did not reach the levels observed in control reindeer which seroconverted within 8 days after they were given the challenge inoculum. One control reindeer aborted at 45 days after challenge exposure (at 165 days of a normal 225-day gestation period), and the fawn of another lived only a few days after delivery. Brucella suis type 4 was isolated from tissues of 3 of 4 control reindeer and of 2 of their fawns. All vaccinated reindeer gave birth to live fawns which were culture negative, although 1 fawn lived only a few days. Brucella suis type 4 was isolated from tissues of 1 vaccinated reindeer. Under the conditions of this experiment, killed B abortus 45/20 vaccine provided increased resistance to brucellosis.  相似文献   

15.
As part of a series on neurological disorders in ruminant livestock in Australia, this review focuses on the congenital neurogenic disorders of cattle. The genetic pressures that contribute to the emergence of congenital neurogenic disorders, as well as the methods of diagnosis, are discussed. Disorders reviewed are ordered by breed and include arthrogryposis multiplex, fawn calf syndrome, inherited congenital myoclonus and maple syrup urine disease.  相似文献   

16.
Polycystic kidney and liver disease was seen in a stillborn white-tailed deer (Odocoileus virginianus) fawn. Bilaterally enlarged kidneys were characterized by severe dilatation of all renal tubules. Glomeruli were sparse, small, and located within a dilated Bowman's capsule. The liver was characterized by marked periportal fibrosis, biliary hyperplasia, and bile duct ectasia with dilated ducts containing inspissated bile. The presentation and morphology of this case are most similar to autosomal recessive polycystic disease in humans.  相似文献   

17.
Neosporosis, caused by the protozoan parasite Neospora caninum, is a serious cause of bovine abortion, stillbirth and perinatal death. This paper reports a clinical neosporosis in a 3-week-old fallow deer (Dama dama). The fawn was full term and appeared normal at birth. Histological lesions consisted of a multifocal necrotizing and granulomatous meningoencephalomyelitis, with intralesional protozoal cysts. Positive immunohistochemical staining and characteristic PCR products confirmed the diagnosis of N. caninum infection.  相似文献   

18.
Adenovirus infection was the cause of an epizootic of hemorrhagic disease that is believed to have killed thousands of mule deer (Odocoileus hemionus) in California during the latter half of 1993. A systemic vasculitis with pulmonary edema and hemorrhagic enteropathy or a localized vasculitis associated with necrotizing stomatitis/pharyngitis/glossitis or osteomyelitis of the jaw were common necropsy findings in animals that died during this epizootic. To study transmission of adenovirus infection in deer and susceptibility of black-tailed deer (Odocoileus hemionus columbianus) fawns to adenovirus infection, six 3-6-month-old black-tailed fawns were divided into two treatment groups. One group was inoculated intravenously and the other group was inoculated through the mucous membranes of the eyes, nose and mouth with purified adenovirus. Each treatment group also included two additional fawns (four total) that were not inoculated but were exposed to inoculated animals (contact animals). One fawn served as a negative control. Between 4 and 16 days postinoculation, 8/10 fawns developed systemic or localized infection with lesions identical to lesions seen in animals with natural disease that died during the epizootic. Transmission was by direct contact, and the route of inoculation did not affect the incubation period or the distribution of the virus (systemic or the localized infection). Immunohistochemical analysis using polyclonal antiserum against bovine adenovirus type 5 demonstrated staining in endothelial cells of vessels in numerous tissues in animals with systemic infection and endothelial staining only in vessels subtending necrotic foci in the upper alimentary tract in animals with the localized form of the disease. All inoculated or exposed animals had staining in the tonsillar epithelium. Transmission electron microscopic examination of lung and ileum from two fawns with pulmonary edema and hemorrhagic enteropathy demonstrated endothelial necrosis and adenovirus virions in endothelial cell nuclei. Adenovirus was reisolated in black-tailed deer pulmonary artery endothelial cells using lung homogenate of the first fawn that developed systemic adenovirus infection. Serum virus neutralization test results suggest that this deer adenovirus is a new serotype.  相似文献   

19.
苇状羊茅91-82新品系品比试验报告   总被引:3,自引:0,他引:3  
选育出的苇状羊茅91-82新品系跟生产上推广种植的进口草种法恩(Fawn)相比,表现出植株高大,生长迅速,最高生长速度可达2.458 cm/d。整个生育期内,其植株高度均明显高于法恩,最高可达155cm,较对照高32.5cm;产草量高,每667m2年产干草可达1 436.77-1 851.67kg,比对照品种法恩(896.82-1 207.22kg)增产53.38%-60.21%,表现出显著的栽培利用价值。结果表明,苇状羊茅91-82是贵州省中南部地区人工草地建植的理想禾本科牧草新品种。  相似文献   

20.
2009年710月,在海南大田国家级自然保护区采用直接观察法对海南赤麂(Muntiacus muntjak ni gripes)的时间分配和活动规律进行了964 h的观察。结果表明,海南赤麂将最多的时间用于采食,其次是反刍和卧息,用于移动和其他行为的时间较少,海南赤麂是以晨昏活动为主的食草动物。性别、年龄和天气均是影响海南赤麂时间分配和活动规律的因素,在时间分配方面,不同性别的个体在反刍和卧息方面存在极显著差异(P<0.01),在采食和移动方面存在显著差异(P<0.05);不同年龄阶段的个体在卧息、移动和其他行为方面存在显著差异(P<0.05);不同天气条件对海南赤麂的移动影响差异显著(P<0.05)。在活动规律方面,不同性别个体的各种行为出现的高峰相似,只是雄性用于卧息的时间较雌性高;不同年龄阶段个体的活动规律差异较大,其中成体和亚成体较为接近,而幼体的活动规律则与成体相反;不同天气条件下海南赤麂的活动规律变化较大,其中受天气因素影响变化最大的是采食、反刍和休息。  相似文献   

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