共查询到20条相似文献，搜索用时 531 毫秒
1.
用水杨酸比色法测定广东佛山某奶牛场K、I 两个群体(27 头)奶牛的血、乳、尿中的酮体,其含量分别为血酮 1.155 mmol/L±0. 511 mmol/L,乳酮 2. 904 mmol/L±1.311 mmol/L,尿酮 3.127 mmol/L±1.814mmol/L。结果表明,其中有 6 头奶牛有隐性酮病, 其乳酮和尿酮的相关系数 r 为-0.59038。 相似文献
2.
用水杨酸比色法测定青海民和县某奶牛场A、B两个群体50头奶牛的血、乳和尿中的酮体,其含量分别为血酮1.163 mmol/L±0.421 mmol/L,乳酮3.041 mmol/L±1.214 mmol/L和尿酮3.232 mmol/L±1.713 mmol/L。结果表明,其中有9头奶牛有隐性酮病,其乳酮和尿酮的相关系数γ=-0.60147。 相似文献
3.
本试验随机选取南宁地区2个奶牛场41头黑白花奶牛进行奶牛酮病的监测研究,试验采用rothera法和水杨醛比色法分别定性和定量检测酮体,采用邻甲苯胺法定量检测血糖。结果表明:①甲奶牛场隐性酮病发生率、可疑隐性酮病发生率分别为20%和15%,乙奶牛场均为零。②甲奶牛场血酮、乳酮、血糖的含量分别为31.1±22.2、78.1±47.2及645.2±79.6 m g/L;乙奶牛场的分别为17.5±8.9、42.6±18.6及637±54.7 m g/L。乳酮及血酮含量在2奶牛场间均存在着极显著差异(P<0.01);而血糖均在正常值范围,两牛场间差异不显著(P>0.05)。最后还讨论分析了奶牛日粮营养,分析发现甲奶牛场日粮粗纤维含量严重不足。 相似文献
4.
为了评价罗氏(Roche)ACCU-CHEK手持血糖仪检测奶牛血浆葡萄糖水平的效果,以及对奶牛酮病的预测价值,选取荷斯坦奶牛场产后1-7周内奶牛,分多次于清晨空腹采集血样200份,应用手持血糖仪现场检测全血血糖含量,分离血浆后送实验室用半自动生化仪检测血浆葡萄糖水平,用酶标仪检测BHBA含量,分析手持血糖仪检测效果以及对酮病的预测情况。血糖仪检测结果(平均值±标准差为3.49±0.57 mmol/L)稍高于生化仪检测结果(3.36±0.58 mmol/L),但两种方法检测的结果之间出现极显著差异(P<0.01)。按照2.8 mmol/L作为健康奶牛血糖下限,血糖仪和生化仪低血糖奶牛检出率(头数)分别为2%(4头)和8.5%(17头)。相关性分析表明,两种血糖方法检测结果为强正相关(n=200,R=0.903,P<0.01)。100头奶牛BHBA检测结果 0.63±0.37 mmol/L,按BHBA>1.2 mmol/L诊断为酮病牛,共检出酮病牛7头,其中血糖仪检为低血糖4头,还有1头奶牛的血糖值为2.9 mmol/L,表明血糖仪可检出约一半以上的酮病牛,可以用于奶牛产后血糖水平的快速监测,对尽早供给能量、预防酮病非常有益。因本实验样本量还较少,今后仍需扩大样本继续试验,为奶牛酮病的早期预测与预防提供科学手段。 相似文献
5.
对示范奶牛场进行1 年酮病监测跟踪调查,对监测的2 794 头次奶牛的血酮值按照不同月份,产后不同阶段,1.20mmol/L≤血酮值＜2.00 mmol/L、血酮值≥2.00 mmol/L的比例,产后每天平均血酮值等进行归类分析,初步掌握了血酮变化情况,为酮病防控提供了技术依据,更具有生产指导意义。 相似文献
6.
奶牛酮病是奶牛产犊后几天至几周内由于碳水化合物及挥发性脂肪酸代谢紊乱引起的一种全身性功能失调的代谢性疾病。以血液、尿、乳中的酮体含量增高,血糖浓度降低,消化机能紊乱,体重减轻,产奶量下降,出现间断性神经症状为特征。奶牛酮病分为临诊酮病和亚临诊酮病。健康牛血清中的酮体(指β-羟丁酸,乙酰乙酸,丙酮)含量一般在1.72mmol/L(100mg/L)以下,亚临诊酮病母牛血清中的酮体含量为1.72~3.44mmol/L(100~200mg/L),而临诊酮病母牛血清中的酮体含量一般都在3.44mmol/L(200mg/L)以上。 相似文献
7.
本文以试剂盒测血清β-羟丁酸含量大于1.2mmol/L作为亚临床酮病奶牛的诊断标准,采用酮粉法、试剂法、ROSS法和试纸法分别对血酮、乳酮和尿酮含量进行检测,并将定性检测的结果与定量检测的结果相比较。结果表明,酮粉法测血酮的符合率最高、为88.54%;试纸法测尿酮次之、为87.50%;ROSS法测尿酮的符合率和阳性率最低,分别为79.17%和13.54%。酮粉法测血酮敏感性最高,为70.37%;ROSS法测尿酮的敏感性最低,为37.04%;试剂法测乳酮、ROSS法测尿酮和试纸法测尿酮三者的特异性均为95.65%,高于酮粉法测血酮的特异性(94.20%),酮粉法测尿酮特异性最低、仅为88.41%。从操作难易度和成本上考虑,酮粉法测定乳酮含量比较适合牧场亚临床酮病的检测。 相似文献
8.
本试验选取90头规模化奶牛场产后0~28d奶牛,采用酮粉法与TNN型奶牛血酮测试仪相结合的方法,调查规模化奶牛场奶牛醋酮血症的发病情况,并以TNN型奶牛血酮测试仪BHBA含量大于1.2mmol/L作为醋酮血症奶牛的诊断标准,采用酮粉法分别对尿、乳和血样品进行检测,并将定性检测的结果与定量检测的结果相比较。结果表明,血酮仪检测β-羟丁酸测得的阳性率为18.89%,与此结果相比较,符合率最高的是酮粉法测血酮,符合率为96.18%;其次是酮粉法测乳酮,符合率为92.47%;酮粉法检测尿酮与乳酮、血酮相比较差异极显著(P0.01);检测尿酮与乳酮结果差异显著不(P0.05)。假阳性率最低的方法是用酮粉法测血酮,假阳性率为9.08%,酮粉法测乳酮为21.06%,而假阳性率最高的是酮粉法测尿酮,为48.56%。从假阳性率分析3种检测方法差异极显著(P0.01)。几种方法中漏检率最低的是酮粉法测乳酮,漏检率为9.11%;其次为酮粉法测尿酮,漏检率为9.18%;漏检率最高的方法是试酮粉法测血酮,漏检率为17.96%。酮粉法测血酮与测尿酮乳酮相比漏检率差异极显著(P0.01)。 相似文献
9.
自1849年Lander首先发现了奶牛酮病的一定神经症状之后,引起了世界许多奶牛业发达国家对酮病的关注。酮病又称酮血病、酮尿病和醋酮血病,它是由于碳水化合物和脂肪代谢紊乱所引起的一种全身功能失调的营养代谢性疾病,其特点是血酮、尿酮、乳酮含量增高,血糖降低(3高1低),消化机能紊乱,泌乳量下降,同时间有神经症状。 相似文献
10.
为了在观察新疆荷斯坦酮病奶牛乳液和血清中β一羟丁酸(BHBA)浓度的相关性。以40头新疆荷斯坦酮病奶牛为试验动物,用酮粉法和改良的水杨醛检测法对其乳样和血清进行检测,将检测为阳性样本按照产奶量和产犊胎次分为2组,每组分别检测乳液和血液中β一羟丁酸浓度,应用磷钨酸沉淀法对乳液进行脱蛋白处理,采用酶荧光法测定乳中β-羟丁酸浓度,应用酶联免疫分析法测定血液中β-羟丁酸浓度。结果:酮病奶牛乳液中β-羟丁酸平均浓度为1.21±0.25 mmol/L;血液中β-羟丁酸平均浓度为2.19±0.35 mmol/L;二者的相关系数在0.7964~0.9105。表明随着产犊胎次的增加,酮病奶牛乳液中的BHBA浓度也逐渐升高,但血液中BHBA浓度变化不显著,其二者的相关系数较高;产奶量越高,乳样和血清中的BHBA含量也在逐渐增高,且二者的相关系数较高。 相似文献
11.
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection. 相似文献
13.
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario. Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously. 相似文献
15.
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
16.
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%). Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada. 相似文献
17.
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
18.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated. Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively. 相似文献
19.
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
20.
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%). Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada. 相似文献
|
