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1.
The chemical composition and protein quality of 11 alternative protein sources (chicken products, blood products, enzyme-hydrolyzed fish protein concentrate, soybean meal, and spray-dried pork liver) were determined, and an experiment was conducted to determine palatability and digestibility of processed red blood cell-containing diets. Chicken protein sources differed in concentrations of CP, acid-hydrolyzed fat, and total AA (TAA) by 20, 31, and 24%, respectively, and GE by 1.7 kcal/g. Blood protein sources varied little in acid-hydrolyzed fat and GE concentrations, but concentrations of CP and TAA differed by 11 and 8%, respectively. Protein solubility of chicken and blood protein source categories averaged 57 and 69%, respectively. Protein solubility of enzyme-hydrolyzed fish protein concentrate, soybean meal, and spray-dried pork liver was 53, 67, and 26%, respectively. Based on calculations from immobilized digestive enzyme assay values, lysine digestibility averaged approximately 80.4 and 81.7% for blood and chicken protein sources, respectively. Lysine digestibility values for soybean meal and spray-dried pork liver were 89 and 77%, respectively. A chick protein efficiency ratio (PER) assay showed that chicken protein sources had high protein quality values, as the PER ranged from 2.7 to 5.3, whereas blood protein sources had poor protein quality (PER values less than 1.5). Enzyme-hydrolyzed fish protein concentrate, spray-dried pork liver, and soybean meal had high protein quality (PER values greater than 2.8). In the dog palatability and digestibility experiments, a corn and chicken-based diet supplemented with either 0 or 3% processed red blood cells was tested. The palatability test showed that dogs consumed more of the diet that contained 0% vs. 3% processed red blood cells. The intake ratio for the 3% processed red blood cells diet was 0.34. Nutrient digestibilities did not differ, except for CP, where the digestibility was greater (P = 0.01) for dogs consuming the 0% processed red blood cells diet. These data suggest that chemical composition and quality of alternative protein sources differ greatly among ingredients within the same category. Palatability data suggest that a processed red blood cells-containing diet is not highly palatable but, when this diet was offered as only one food, dogs demonstrated no aversion response but some decrease in protein digestion.  相似文献   

2.
An experiment to determine the chemical composition and protein quality of 13 fish substrates (pollock by-products, n = 5; fish protein hydrolysates, n = 5; and fish meals, n = 3) was conducted. Two of these substrates, salmon protein hydrolysate (SPH) and salmon meal with crushed bones (SMB), were used to determine their palatability as components of dog diets. Pollock by-products differed in concentrations of CP, crude fat, and total AA by 71, 79, and 71%, respectively, and GE by 4.1 kcal/g. Fish protein hydrolysates and fish meals were less variable (approximately 18, 14, and 17%, and 1.4 kcal/g, respectively). Biogenic amine concentrations were much higher in fish protein hydrolysates as compared with pollock by-products and fish meals. Pollock liver and viscera had the highest total fatty acid concentrations; however, red salmon hydrolysate and SMB had the highest total PUFA concentrations (49.63 and 48.60 mg/g, respectively). Salmon protein hydrolysate had the highest protein solubility in 0.2% KOH. Based on calculations using immobilized digestive enzyme assay values, lysine digestibility of fish meal substrates was comparable to in vivo cecectomized rooster assay values and averaged approximately 90.3%. Also, pollock milt, pollock viscera, red salmon hydrolysate, and sole hydrolysate had comparable values as assessed by immobilized digestive enzyme assay and rooster assays. A chick protein efficiency ratio (PER) assay compared SMB and SPH to a whole egg meal control and showed that SMB had high protein quality (PER = 3.5), whereas SPH had poor protein quality (PER value less than 1.5). However, using whole egg meal as the reference protein, both fish substrates were found to be good protein sources with an essential AA index of 1.0 and 0.9 for SMB and SPH, respectively. In the dog palatability experiments, a chicken-based control diet and 2 diets containing 10% of either SPH or SMB were tested. Dogs consumed more of the SPH diet compared with the control, and similar amounts of the SMB and control diets. The intake ratios for each were 0.73 and 0.52, respectively. Salmon protein hydrolysate was especially palatable to dogs. These data suggest that chemical composition and nutritional quality of fish substrates differ greatly and are affected by the specific part of the fish used to prepare fish meals and fish protein hydrolysates.  相似文献   

3.
Background: The study aimed to determine the apparent total tract digestibility coefficients(ATTDC) of nutrients, the apparent metabolizable energy(AME and AMEn) and the amino acid(AA) apparent ileal digestibility coefficients(AIDC)of a partially defatted(BSFp) and a highly defatted(BSFh) black soldier fly larvae meal. The experimental diets were: a basal diet and two diets prepared by substituting 250 g/kg(w/w) of the basal diet with BSFp or BSFh, respectively.Results: Significant differences were found between BSFp and BSFh meals for ATTDC of the nutrients: BSFp resulted more digestible than BSFh, except for ATTDC of CP which did not differed between meals, while a statistical trend was observed for ATTDC of DM and EE. The AME and AMEn values were significantly(P 0.05) different between the two BSF meals, with higher levels for BSFp(16.25 and 14.87 MJ/kg DM, respectively). The AIDC of the AA in BSFp ranged from 0.44 to 0.92, while in BSFh they ranged from 0.45 to 0.99. No significant differences were observed for the AA digestibility(0.77 and 0.80 for BSFp and BSFh, respectively), except for glutamic acid, proline and serine that were more digestible in the BSFh meal(P 0.05).Conclusions: Defatted BSF meals can be considered as an excellent source of AME and digestible AA for broilers with a better efficient nutrient digestion. These considerations suggested the effective utilization of defatted BSF larvae meal in poultry feed formulation.  相似文献   

4.
This study evaluated the effect of two rendering processes applied to meat and bone meal (MBM) production, associated or not with extrusion, by measuring diet apparent total tract digestibility of dogs and cats, and true digestibility of amino acids (AA) by cecectomized roosters. Four diets were evaluated, with the main protein source as follows: conventional and extruded MBM; high temperature and pressure (HPT; 135 °C, 3 bar, 20 min) and extruded MBM; conventional non‐extruded MBM; HPT non‐extruded MBM. Nutrient digestibility and food metabolizable energy content were evaluated with dogs and cats by the method of total collection of faeces. True AA digestibility was evaluated by a precision‐fed assay with cecectomized roosters. The evaluated MBM had high‐ash content. The HPT process of MBM increased the digestibility of crude protein of the diets by cats, and the true digestibility of several AA by cecectomized roosters. The extrusion process did not modify the apparent total tract nutrient digestibility of MBM by dogs and their amino acid digestibility by roosters, but increased the digestibility of the dietary protein by cats. Dogs fed HPT MBM presented a higher urea post‐prandial response, suggesting reductions in bioavailability and protein synthesis from absorbed AA.  相似文献   

5.
Black soldier fly larvae (BSFL) are an alternative protein source for animals, including dogs and cats. Dietary calcium source is an essential nutrient for BSFL development in the pupal stage. Calcium carbonate (CaCO3) and calcium chloride (CaCl2) are common calcium sources but differ in solubility, acid-binding capacity, and calcium concentration. A high calcium concentration in BSFL may affect how well nitrogen and amino acids (AA) are digested by animals consuming them, thereby affecting feed conversion efficiency. Our objective was to determine the effects of dietary calcium form and concentration on nutrient composition, AA digestibility, and digestible indispensable amino acid score (DIAAS)-like values of BSFL intended for use in animal feeds using the precision-fed cecectomized rooster assay. All BSFL tested in this study were harvested at 18 d after hatch. Industry standard rearing conditions were maintained and a commercial layer ration was fed to all BSFL until 11 d post-hatch. From day 11 to 18, BSFL were fed a combination of distiller’s dried grains with solubles from a distillery, bakery byproduct meal, and varied calcium sources. All BSFL diets contained 0.2% calcium in the basal diet plus additional calcium in the following amounts and forms: BSFLA: 1.2% CaCl2, BSFLB: 1.2% CaCO3, BSFLC: 0.75% CaCO3, and BSFLD: 0.6% CaCO3 + 0.6% CaCl2. On day 18, BSFL were washed and frozen. Prior to the rooster assay, BSFL were lyophilized and ground. In total, 16 cecectomized roosters (4 roosters per substrate) were randomly assigned to test substrates. After 24 h of feed withdrawal, roosters were tube-fed 20 g of test substrates. Following crop intubation, excreta were collected for 48 h. Endogenous corrections for AA were made using five additional cecectomized roosters. All data were analyzed using a completely randomized design and the GLM procedure of SAS 9.4. Nutrient and AA digestibilities were not different among substrates. DIAAS-like values were calculated to determine protein quality according to the Association of American Feed Control Officials nutrient profiles and National Research Council recommended allowances for dogs and cats. Although AA digestibilities did not differ, those containing CaCO3 generally had higher DIAAS-like reference values than the diet containing CaCl2 alone (BSFLA). Aromatic AA (Phe + Tyr) and sulfur AA (Met + Cys) were often first-limiting AA. Our results suggest that calcium sources fed to BSFL did not affect AA digestibility and protein quality.  相似文献   

6.
1. The objectives were to study the variation in amino acid (AA) digestibility of rapeseed meal in laying hens and to investigate whether the variation in AA digestibility can be explained by chemical constituents of the rapeseed meal. Nine rapeseed meals from different processing plants operating in Germany were used. The crude protein and crude fibre concentrations of the meals ranged from 367 to 410 and 137 to 175?g/kg dry matter (DM), respectively. The concentrations of total glucosinolates varied between 5·1 and 12·9?mmol/kg DM. Each meal was included in one of the experimental diets at an inclusion level of 200?g/kg at the expense of maize starch.

2. A total of 16 Lohmann Brown laying hens were used for this experiment and were surgically caecectomised. They were housed individually in metabolism cages. Each of the 10 diets was fed to at least 6 hens in 4 periods of two weeks duration. Excreta were collected during the last 5?d of each period. The digestibility coefficient of AA was calculated for each diet. The digestibility of the rapeseed meals was calculated by multiple linear regression analysis.

3. Mean essential AA digestibility of rapeseed meals varied between 0·78 and 0·84. Among the essential amino acids, the highest level of digestibility was found for arginine (0·88) and methionine (0·87), and the lowest for lysine (0·74) and threonine (0·75). The highest variation between rapeseed meals in digestibility was found for threonine (0·68 to 0·79) and lysine (0·68 to 0·78). The concentration of neutral detergent insoluble nitrogen of the rapeseed meals was negatively correlated with the digestibility of lysine, but was not significantly correlated with digestibility of any other AA. Glucosinolate concentration was significantly correlated with valine digestibility.

4. Multiple regression analysis showed that the concentrations of crude protein and ash together were the major factors considered to explain variation in digestibility of essential AA. Additional consideration of neutral detergent insoluble nitrogen and total glucosinolates improved the goodness of fit only marginally and was unlikely to be of practical significance.  相似文献   

7.
This experiment was designed to evaluate the effects of selected soybean (SB) processing byproducts (gums, oil, soapstock, weeds/trash) when added back to soybean meal (SBM) during processing on the resulting nutrient composition, protein quality, nutrient digestibility by swine, and true metabolizable energy (TMEn) content and standardized AA digestibility by poultry. To measure ileal DM and nutrient digestibility, pigs were surgically fitted with a T-cannula in the distal ileum. The concentration of TMEn and the standardized AA digestibility by poultry were determined using the precision fed cecectomized rooster assay. Treatments in the swine experiment included SBM with no by-products; SBM with 1% gum; SBM with 3% gum; SBM with 0.5% soapstock; SBM with 1.5% soapstock; SBM with 2% weeds/trash; SBM with a combination of 3% gum, 1.5% soapstock, and 2% weeds/trash; SBM with 5.4% soybean oil; and roasted SB. A 10 x 10 Latin square design was utilized. The experiment was conducted at the University of Illinois, Urbana-Champaign, and at The Ohio State University, Columbus. In the swine experiment, apparent ileal DM, OM, CP, and AA digestibilities were reduced (P < 0.05) when pigs consumed the combination by-product diet compared with the diet containing no by-products. Apparent ileal digestibilities of DM, CP, and total essential, total nonessential, and total AA were lower (P < 0.05) for any diet containing by-products compared with the diet with no by-products. Apparent ileal digestibilities of DM, OM, CP, and AA were lower (P < 0.05) for the roasted SB-compared with the SB oil-containing diet. In the rooster experiment, TMEn values were greater (P < 0.05) for roasted SB compared with SBM with no by-products and increased linearly as the addition of soapstock increased. Individual, total essential, total nonessential, and total AA digestibilities were lower (P < 0.05) for roosters fed roasted SB versus SBM devoid of by-products. Gums, soapstock, and weeds/trash reduce the nutritive value of the resultant meal when they are added back during processing.  相似文献   

8.
Amino acid composition of meat-and-bone meals, poultry by-product meals, blood meals, bone meals and feather meals showed characteristic differences. Meat-and-bone meals and blood meals had surplus in lysine, whereas poultry by-product meals and feather meals were relatively rich in cystine. Blood meals had high levels of branched-chain amino acids as compared to isoleucine. In vitro pepsin digestibility of meat-and-bone meals (79.9 +/- 17.7%; n = 24) and blood meals (95.8 +/- 4.2%; n = 11) was found to be higher than that of poultry by-product meals (65.3 +/- 7.7%; n = 14) or feather meals (44.7 +/- 9.2%; n = 16). Pepsin digestibility of poultry by-product meals showed a significant negative correlation with crude protein content (r = -0.73; P less than 0.05; n = 14). However, in vitro pepsin digestibility of poultry by-product meals as well as meat-and-bone meals, blood meals and feather meals, showed insignificant correlations with NPU indices as well as with available crude protein contents of the meals. The NPU values of meat-and-bone meals for rats (29.9 +/- 11.7; n = 100) were lower than those of poultry by-product meals (52.1 +/- 7.1; n = 14). The NPU values of blood meals (6.4 +/- 5.6; n = 11) and feather meals (23.5 +/- 10.0; n = 16), determined as sole sources of protein, were low and they did not elicit weight gain in rats. The available crude protein content of poultry by-product meals (34.3 g/100 g +/- 3.6; n = 14) was higher than that of meat-and-bone meals (17.0 g/100 g +/- 7.3; n = 100).(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

9.
Eight digestion trials were conducted with pigs fitted with ileal T-cannulas to determine the apparent digestibilities of N and amino acids in common protein feedstuffs. Trial-to-trial variation was minimal as determined by the variation in digestibilities for soybean meal, which was evaluated in each trial. Apparent digestibilities varied widely; ileal N digestibility ranged from 88 to 63%, and ileal lysine digestibility ranged from 93 to 40%. In general, ring-dried blood meal, corn gluten meal, Menhaden fish meal, poultry-by-product meal and extruded whole soybeans were the most digestible, followed by canola meal, sunflower meal, peanut meal and meat and bone meal. Cottonseed meal and feather meal were the least digestible. Mean values for each feedstuff agree well with published data. Variation in digestibilities among samples of the same feedstuff was greater for the meat and bone meals. Regression of ileal essential amino acid digestibilities on ileal and fecal N digestibility indicated that amino acid digestibilities can be predicted more precisely from ileal N digestibility than from fecal N digestibility. However, neither ileal nor fecal N digestibility could be used with a high degree of certainty to predict ileal amino acid digestibilities.  相似文献   

10.
The digestibility of AA in dry extruded-expelled soybean meal (DESBM) and regular, solvent-extracted soybean meal (SBM) were determined in pigs and poultry. In the pig assay, 4 Cotswold barrows (average initial BW of 80.4 kg) fitted with a T-cannula at the distal ileum were allotted to 4 semipurified diets in a 4 x 4 Latin square design. Diet 1, a low protein diet (5% casein), was used to quantify endogenous CP and AA losses. Diets 2, 3, and 4 were formulated to contain 35% regular, solvent-extracted SBM; batch 1 of DESBM (DESBM-1); and batch 2 of DESBM (DESBM-2), respectively, as the sole source of protein. The DESBM samples were obtained from 2 different batches but were subjected to the same processing conditions. Chromic oxide (0.3%) was included as a digestibility marker in all diets. Compared with DESBM-1 and DESBM-2, apparent ileal digestibility of DM in SBM was greater (P < 0.05). Apparent and true ileal digestibilities of AA in SBM were greater (P < 0.05) compared with DESBM-2. In the poultry assay, 4 dietary treatments were each assigned to adult cecectomized roosters in a completely randomized design. Treatment 1 was a nonnitrogenous diet (NND; 90% sucrose and 10% vegetable oil) used to estimate endogenous N and AA losses. Treatments 2, 3, and 4 contained SBM, DESBM-1, and DESBM-2 as the only source of protein. Each of these diets was fed in 25-g quantities formulated to provide 5 g of CP from the respective soybean meal source. The SBM had greater (P < or = 0.05) true digestibility for isoleucine, leucine, cysteine, proline, serine, and tyrosine compared with DESBM-1. The results indicate that, relative to regular, solvent-extracted soybean meal, AA digestibilities of different batches of dry extruded-expelled soybean meal varied in pigs and poultry.  相似文献   

11.
试验比较了不同热处理时间大豆片、普通豆粕和去皮豆粕在生长猪回肠表观和真消化率的影响。试验采用12头回肠末端安装简单T型瘘管的生长猪,6×6有重复拉丁方设计。采用玉米淀粉-豆粕半纯合型日粮,配制酪蛋白日粮用以测定内源氮和氨基酸的损失量。结果表明:随着大豆片热处理时间的延长(0~18min),粗蛋白质和氨基酸的回肠表观和真消化率均显著提高(线性效应,P<0.01)。生大豆片的粗蛋白质和氨基酸的消化率显著低于普通豆粕和去皮豆粕(P<0.01),但普通豆粕与去皮豆粕间差异不显著(P>0.05)。  相似文献   

12.
The apparent ileal digestibility of amino acids in 8 feed ingredients was determined using 14-, 28- and 42-d-old male broiler chickens. The ingredients included three cereals (wheat, sorghum and maize), one cereal by-product (mill run), three oilseed meals (canola, cottonseed and soybean meals) and one animal protein meal (meat and bone meal). Dietary crude protein in the assay diets was supplied solely by the test ingredient. All diets contained 20 g/kg acid-insoluble ash as an indigestible marker, and each diet was offered ad libitum in mash form to 5 replicate pens from 11 to 14 d, 25 to 28 d and 39 to 42 d post-hatching. There were 12, 6 and 6 birds per pen for the 14, 28 and 42 d samplings, respectively. The results suggest that the age of broilers significantly influenced the apparent ileal digestibility of amino acids. The effects, however, varied among amino acids and ingredients. In wheat, the digestibility of most amino acids was higher in 14- than in 28- and 42-d-old broilers. In maize, the digestibility coefficients of amino acids were higher at 28 and 42 d than at 14 d. The digestibility coefficients in maize and wheat at 28 and 42 d were similar. The digestibility of amino acids in sorghum at 42 d was higher than those at 28 d, but similar to those at 14 d except for histidine, lysine, serine and glycine, which were significantly higher at 42 d. Digestibility of amino acids in sorghum was similar between 14 and 28 d except for isoleucine, leucine, phenylalanine, glutamic acid and alanine. The digestibility of amino acids in mill run at 42 d was significantly higher than those at 14 and 28 d. There were no differences in digestibility between 14 and 28 d. In general, the digestibility of amino acids in canola meal, soybean meal and, meat and bone meal was higher at 28 and 42 d compared to those at 14 d, and similar between 28 and 42 d of age. In cottonseed meal, age had no effect on the digestibility coefficient of amino acids, except for lysine and arginine, which increased with age. Analysis of the combined results for the 8 feed ingredients showed that, in general, the digestibility coefficients of amino acids increased with advancing age of broiler chickens.  相似文献   

13.
1. The apparent ileal and excreta digestibilities of amino acids in 15 samples representing 12 food ingredients were determined using 5-week-old male broiler chickens. The ingredients included 3 samples of cereals (wheat, maize and sorghum), 6 samples of plant protein meals (soyabean meal, cottonseed meal, canola meal and sunflower meal) and 6 samples of animal protein meals (meat meal, meat-and-bone meal, feather meal and fish meal). 2. The test ingredients were incorporated as the sole source of dietary protein in assay diets. Each diet was offered ad libitum to 3 pens (4 birds/pen) from d 35 to d 42 post-hatching. Total collection of excreta was carried out during the last 4 d. All birds were killed on d 42 and the contents of the lower half of the ileum were collected. Apparent ileal and excreta amino acid digestibilities were calculated using acid-insoluble ash as the indigestible marker. 3. The influence of site of measurement was found to vary among food ingredients, among samples within an ingredient and among different amino acids within an ingredient. Ileal amino acid digestibility values were similar in some ingredients, but significantly lower or higher in others than the corresponding excreta values. 4. Average ileal and excreta amino acid digestibilities in sorghum and maize were similar, but significant differences were observed for individual amino acids. In contrast, ileal amino acid digestibility values were higher than the corresponding excreta digestibility values in wheat. 5. The average ileal and excreta digestibilites of amino acids in the 3 soyabean meal samples were similar although small, but significant differences were noted for individual amino acids. Site of measurement had no effect on the digestibility of amino acids in canola meal. Digestibilities of valine, isoleucine, phenylalanine, histidine, glutamic acid, alanine and tyrosine in sunflower meal and those of valine, methionine, isoleucine, leucine, lysine, glutamic acid and alanine in cottonseed meal were lower by excreta analysis. 6. Digestibilities in animal protein meals, with the exception of blood meal and fish meal, were consistently higher by excreta analysis. Ileal-excreta differences in individual amino acid digestibilities were more evident in feather meal, meat meal and meat-and-bone meal. 7. Threonine and valine were the indispensable amino acids that were more frequently influenced by the site of measurement. Of the dispensable amino acids, aspartic acid, serine, glutamic acid and alanine were the most affected. 8. Differences determined between ileal and excreta digestibilities in the present study clearly demonstrate that amino acid metabolism by hindgut microflora in chickens may be substantial and that digestibilities measured in the terminal ileum are more accurate measures of amino acid availability than those measured in the excreta.  相似文献   

14.
Recent advances in swine protein nutrition are characterized by the development of functional amino acids (AA) in regulating fetal and postnatal survival, growth and development. These AA include arginine, glutamine, glutamate, proline, leucine, cysteine and tryptophan. Due to limited knowledge on AA nutrition, pork producers have traditionally paid little attention to supplementing the arginine family of AA to swine diets. Results of recent studies indicate that functional AA serve important regulatory functions in nutrient metabolism, protein turnover, and immune function, therefore enhancing efficiency of feed utilization by pigs. The underlying mechanisms include activation of nitric oxide, mammalian target of rapamycin, gaseous signaling, and AMP-activated protein kinase pathways, as well as anti-oxidative function. Dietary supplementation with arginine, glutamine, proline or leucine to weanling piglets enhances their growth performance. Arginine or glutamine is also effective in increasing milk production by lactating sows. Furthermore, supplementing arginine to the diet of pregnant gilts between days 30 and 114 of gestation increases the number of live-born piglets and litter birth-weight. Availability of feed-grade functional AA holds great promise for improving animal health and nutrient utilization in pig production worldwide. Additionally, feedstuffs of animal origin [e.g., blood meal (ring dried), feather meal (hydrolyzed), meat and bone meal, porcine protein meal, and poultry by-product meal (both feed- and petfood-grades)] are excellent and cost-effective sources of both essential and functional AA for formulating balanced swine diets. New knowledge on AA nutrition provides a much needed scientific basis for revising the next edition of swine nutrient requirements.  相似文献   

15.
Recent advances in swine protein nutrition are characterized by the development of functional amino acids(AA)in regulating fetal and postnatal survival,growth and development. These AA include arginine,glutamine,glutamate,proline,leucine, cysteine and tryptophan. Due to limited knowledge on AA nutrition,pork producers have traditionally paid little attention to supplementing the arginine family of AA to swine diets. Results of recent studies indicate that functional AA serve important regulatory functions in nutrient metabolism,protein turnover, and immune function,therefore enhancing efficiency of feed utilization by pigs. The underlying mechanisms include activation of nitric oxide,mammalian target of rapamycin,gaseous signaling,and AMP-activated protein kinase pathways, as well as anti-oxidative function. Dietary supplementation with arginine,glutamine, proline or leucine to weanling piglets enhances their growth performance. Arginine or glutamine is also effective in increasing milk production by lactating sows. Furthermore,supplementing arginine to the diet of pregnant gilts between days 30 and 114 of gestation increases the number of live-born piglets and litter birth-weight. Availability of feed-grade functional AA holds great promise for improving animal health and nutrient utilization in pig production worldwide. Additionally, feedstuffs of animal origin[e. g. , blood meal (ring dried),feather meal(hydrolyzed),meat and bone meal, porcine protein meal,and poultry by-product meal(both feed- and petfood-grades )]are excellent and cost-effective sources of both essential and functional AA for formulating balanced swine diets.New knowledge on AA nutrition provides a much needed scientific basis for revising the next edition of swine nutrient requirements.  相似文献   

16.
1. This study investigated the net disappearance of crude protein (CP) and amino acids (AA) from soybean meal and rapeseed meal in different sub-sections of the ileum of hens. A basal diet was used and in another 4 diets, maize starch contained in the basal diet was replaced with soybean meal or rapeseed meal at 140 and 280 g/kg so that changes in dietary AA concentrations resulted from the oilseed meals only. 2. A total of 250 Lohmann Brown pullets were used for this experiment at 20 weeks old and given one of the 5 diets for 7 d. The section between Meckel's diverticulum and 2 cm anterior to the ileo-caecal-colonic junction was taken after slaughtering the hens and cut into three sub-sections of equal length. Digesta were taken separately from each sub-section and then frozen. Net disappearance of AA and CP for the diets and the two protein sources was calculated based on standard equations and using a multiple linear regression approach. 3. Net disappearances of CP and all AA were significantly lower in the proximal sub-section than in the central and terminal sub-sections. Also, the net disappearance of CP and all AA from rapeseed meal was significantly lower in the proximal sub-section than in the central or terminal sub-sections. For soybean meal, only the net disappearances of arginine, aspartic acid, glutamic acid and phenylalanine were significantly lower in the proximal than in the terminal sub-section. No significant differences in the net disappearance of CP and AA were detected between protein sources within the central and terminal sub-sections or between the central and terminal sub-section within each protein source. Soybean meal had a significantly higher net disappearance of CP and AA (with the exception of cystine and methionine) than rapeseed meal in the proximal sub-section but these differences were not statistically significant in the central and terminal sub-sections. 4. Amino acids disappeared from the proximal region of the ileum. This needs consideration in standard approaches that are used for measuring AA digestibility. There is a variation in AA digestibility between soybean meal and rapeseed meal. The ranking of individual AA regarding their digestibility is different between the two oilseed meals.  相似文献   

17.
体外消化过程中蛋白质品质与寡肽释放的研究   总被引:14,自引:0,他引:14  
应用胃蛋白酶 胰酶两步体外消化法测定 6种豆饼 (粕 )、3种鱼粉、菜籽饼、玉米蛋白粉、肉粉、蚕蛹、酪蛋白等 1 4种蛋白质饲料的寡肽释放量 ,并研究了它们与蛋白质质量和氨基酸组成间的关系。结果表明 :酪蛋白、鱼粉、豆饼 (粕 )的寡肽释放量最高 ,贮藏的鱼粉及加热、贮藏过的豆粕、饼寡肽释放量降低 ;寡肽释放量与饲料赖氨酸、有效赖氨酸、异亮氨酸呈正相关 (r =0 .86~ 0 .93,P <0 .0 1 )。鱼粉、豆饼、蚕蛹、菜子饼寡肽平均链长在 5~ 6之间 ,新鲜豆粕、酪蛋白、玉米蛋白粉链长为 7.5、8.4、1 1 .4 ,大分子肽含量较高 ;植物饲料的寡肽中非必需氨基酸 (NEAA)占总氨基酸的 5 0 %~ 70 % ,必需氨基酸 (EAA)相对较低 ;动物性饲料NEAA比例仅略高于EAA。所有饲料释放的FAA不到总氨基酸的 1 % ,其中主要是EAA ,饲料中含有效碱性氨基酸的真蛋白与饲料总真蛋白的比值 (CBBG/TP)与寡肽和FAA的释放量呈显著线性相关 (r=0 .92和 0 .87) ,寡肽释放量和CBBG/TP均较好地反映了蛋白质质量。  相似文献   

18.
青鱼对8种饲料原料中营养物质的表观消化率   总被引:1,自引:0,他引:1  
本试验旨在研究青鱼(Mylopharyngodon piceus)对国产鱼粉、蝇蛆粉、玉米蛋白粉、大豆粕、花生粕、棉籽粕、菜籽粕和米糠8种饲料原料干物质、粗蛋白质、氨基酸、粗脂肪、总磷和总能的表观消化率。试验饲料由70%基础饲料和30%待测饲料原料组成,并以0.1%的三氧化二钇(Y2O3)为外源指示剂。选取平均体重为(60.17±1.28)g的青鱼270尾,随机分成9组,每组3个重复,每个重复10尾鱼。对照组试验鱼饲喂基础饲料,试验组试验鱼分别饲喂1种试验饲料。饲喂1周后采用自排法收集粪便待测。结果表明:8种饲料原料的干物质、粗蛋白质、粗脂肪、总磷、总能以及总氨基酸的表观消化率的范围分别是62.17%~86.47%、83.34%~95.84%、78.93%~100.06%、37.33%~81.99%、66.75%~89.86%、86.33%~96.58%。在8种饲料原料中,玉米蛋白粉的干物质、总磷和总能的表观消化率最高,而大豆粕的粗蛋白质、粗脂肪和总氨基酸的表观消化率最高;棉籽粕的干物质和总能的表观消化率最低,米糠的粗脂肪和总磷的表观消化率最低。各饲料原料中总氨基酸表观消化率与粗蛋白质表观消化率的变化趋势一致,其中以蝇蛆粉最低。由此可见,大豆粕和玉米蛋白粉是青鱼的优质植物蛋白质源,可适量替代鱼粉;蝇蛆粉中粗蛋白质和氨基酸的消化率都较低,在青鱼饲料中的添加量不宜过高;花生粕、棉籽粕和菜籽粕也是较好的植物性蛋白质源,在青鱼饲料中适量添加既有利于饲料的营养平衡,还可降低饲料成本;米糠作为青鱼的能量原料必须保持新鲜,并控制其在饲料中的用量。  相似文献   

19.
浙江鱼粉和秘鲁鱼粉氨基酸对鸡的消化率比较   总被引:1,自引:0,他引:1  
本试验以去盲肠鸡和正常鸡各16只测定了浙江鱼粉和秘鲁鱼粉的氨基酸表观消化率(AAAD)和真消化率(TAAD)。结果表明,以去盲肠鸡测定,浙江鱼粉多数氨基酸的AAAD和TAAD超过秘鲁鱼粉(P<0.05)。以正常鸡测定,两者大多数氨基酸消化率无显著差异(P>0.05)。说明新鲜优质浙江鱼粉的氨基酸可消化性与进口鱼粉相当,甚至更优  相似文献   

20.
The objectives of this experiment were to determine the apparent ileal digestibility and the standardized ileal digestibility (SID) of CP and AA in bakery meal, corn gluten meal, corn gluten feed, corn germ meal, and hominy feed and to compare these values with the apparent ileal digestibility and SID of CP and AA in corn and distillers dried grains with solubles (DDGS). Eight growing barrows (initial BW: 82.5 ± 5.5 kg) were randomly allotted to an 8 × 8 Latin square design with 8 diets and 8 periods. Diets contained corn, DDGS, bakery meal, corn gluten meal, corn gluten feed, corn germ meal, or hominy feed as the sole source of protein and AA. An N-free diet was used to measure basal endogenous losses of AA and protein. Pigs were fed experimental diets for eight 7-d periods, with ileal digesta being collected on d 6 and 7 of each period. Results indicated that the SID of Lys in corn gluten meal (78.7%) was greater (P < 0.01) than in DDGS, bakery meal, corn germ meal, and hominy feed (46.0, 48.4, 68.4, and 58.8%, respectively). The SID of all indispensable AA except Arg, Leu, and Met in bakery meal were not different from those in DDGS. The SID of Arg, His, Leu, and Met in corn gluten feed were less (P < 0.01) than in corn, but the SID of all other indispensable AA in corn gluten feed were not different from those in corn. However, for most indispensable AA, the SID in corn gluten feed was not different from the SID in DDGS. The SID of all indispensable AA in corn germ meal, except Arg, His, Leu, and Met, were not different from the SID in corn. Likewise, the SID of all indispensable AA in corn germ meal, except Arg and Leu, were not different from those in DDGS. The SID of Ile, Met, Phe, and Val in hominy feed were less (P < 0.01) than in corn, but the SID of the remaining indispensable AA in hominy feed were not different from the SID of indispensable AA in corn. All indispensable AA in hominy feed also had SID values that were not different from the SID values of AA in DDGS, except for Arg and Lys, which had greater (P < 0.01) SID than in DDGS. In conclusion, bakery meal had SID values of most AA that were less than in corn, but corn gluten meal had SID values for most AA that were greater than the SID of AA in corn, bakery meal, and corn coproducts.  相似文献   

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