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1.
Vilcek S Durkovic B Kolesarova M Paton DJ 《Preventive veterinary medicine》2005,72(1-2):31-5; discussion 215-9
Genetic typing of bovine viral diarrhoea virus (BVDV) is important for the precise classification of viruses as well as for the development of molecular epidemiology. BVDV isolates were usually typed based on comparison of genomic sequences from the 5'-untranslated region (5'-UTR), N(pro) and E2 region. Recently we have identified 11 genetic groups (subgenotypes) of BVDV-1. Our further experiments confirmed a new subgenotype, BVDV-1k, isolated from cattle in Switzerland. BVDV isolates from India were typed as BVDV-1b whereas BVDV-1c is a predominant subgenotype in Australia. The results of genetic typing of BVDV indicate that distribution of subgenotypes has no relationship to the geographic origin of viral isolates. 相似文献
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Mahony TJ McCarthy FM Gravel JL Corney B Young PL Vilcek S 《Veterinary microbiology》2005,106(1-2):1-6
Eighty-nine bovine viral diarrhoea viruses (BVDV) from Australia have been genetically typed by sequencing of the 5' untranslated region (5'-UTR) and for selected isolates the N(pro) region of the viral genome. Phylogenetic reconstructions indicated that all of the samples examined clustered within the BVDV type 1 genotype. Of the 11 previously described genetic groups of BVDV-1, 87 of the samples examined in this study clustered with the BVDV-1c, while two samples clustered with the BVDV-1a. Based on these analyses there appears to be limited genetic variation within the Australian BVDV field isolates. In addition, the phylogenetic reconstructions indicate that the clustering of Australian BVDV in the phylogenetic trees is not a result of geographic isolation. 相似文献
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Mishra N Pattnaik B Vilcek S Patil SS Jain P Swamy N Bhatia S Pradhan HK 《Veterinary microbiology》2004,104(3-4):207-212
Thirteen BVDV isolates collected in four geographic regions of India between 2000 and 2002 were typed in 5'-UTR. To confirm results of genetic typing, selected viruses were also analysed in the N(pro) region. Phylogenetic analysis revealed that all Indian BVDV isolates belong to BVDV-1b (Osloss-like group). Despite a long distance between the farms from which the viruses were isolated there was no correlation between the origin of viral isolates and their position in a phylogenetic tree. Higher genetic similarity of Indian BVDV isolates was observed most probably due to the uncontrolled movement of cattle as well as the uncontrolled use of semen from bulls for breeding of local and farm cattle in different states of India. 相似文献
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分析2013—2019年中国西北部分省区不同基因亚型牛病毒性腹泻病毒（BVDV）抗原基因Erns的分子特征,了解其遗传演化规律。从甘肃、青海、宁夏规模化牛场送检的疑似牛病毒性腹泻发病牛150份EDTA抗凝血提取总RNA,利用RT-PCR扩增病毒基因组Erns-E1区,克隆测序后比对,构建系统进化树进行遗传演化关系分析。利用牛肾细胞MDBK对检出的不同基因亚型BVDV进行分离,并鉴定其生物型。RT-PCR扩增结果表明,BVDV总体阳性率为37.33%,其中甘肃省、青海省、宁夏回族自治区BVDV阳性率分别为37.68%、35.71%、40.00%。获得56份Erns-E1 DNA,克隆测序获得33条不同的Erns序列,长度均为681 bp,分析表明流行株分属10个BVDV基因亚型：BVDV-1a （2株）、BVDV-1b （5株）、BVDV-1c （1株）、BVDV-1d （3株）、BVDV-1m （11株）、BVDV-1o （1株）、BVDV-1p （4株）、BVDV-1q （4株）、BVDV-1v （1株）、BVDV-2a （1株）。分离获得BVDV-1a亚型、BVDV-1b亚型、BVDV-1v亚型、BVDV-2a亚型分离株各1株,BVDV-1 d亚型分离株2株,均为非致细胞病变型。各亚型株间Erns基因核苷酸相似性以BVDV-1a～1d经典亚型株（79.8%～85.9%）或1m～1q及1v新亚型株（81.0%～87.3%）较高,以BVDV-1 m和BVDV-1p流行株亚型间相似性最高（87.3%）。各亚型株Erns基因编码蛋白的RNA酶活性位点以及双链RNA作用基序（139KKGK142）保守,但Erns第26位糖基化位点（26 NRSL）在1m～1q、1v亚型株移位（24 NVSR）。首次以Erns核苷酸序列构建系统进化树,结果显示1m～1q及1v等亚型BVDV株在进化上关系较为密切。本研究首次选用Erns靶标基因对甘肃、青海、宁夏部分省区牛源BVDV株进行同源性及系统进化分析,发现10个基因亚型流行株,以1m亚型株最为普遍,1m～1q及1v等亚型株亲缘关系密切。 相似文献
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Genetic heterogeneity of bovine viral diarrhoea virus (BVDV) isolates from Turkey: identification of a new subgroup in BVDV-1 总被引：1，自引：0，他引：1
Kadir Y Christine F Barbara BW Zeki Y Feray A Aykut O Ibrahim B Sibilina Cedillo R Heinz-Jürgen T Matthias K 《Veterinary microbiology》2008,130(3-4):258-267
Genetic heterogeneity of Turkish ruminant pestiviruses was investigated by phylogenetic analysis of complete N(pro) encoding nucleotide sequences. A total of 30 virus isolates obtained from 15 provinces around the country between 1997 and 2005 were included in the phylogenetic analysis. Virus isolates mostly originated from cattle with one isolate from sheep. The bovine isolates all belonged to BVDV-1, the sheep isolate to BVDV-2. Fifteen isolates formed a new subgroup within BVDV-1, tentatively named BVDV-1l. The remaining bovine isolates were typed as BVDV-1a (n=4), BVDV-1b (n=4), BVDV-1d (n=3), BVDV-1f (n=2) and BVDV-1h (n=1). The isolates allocated to BVDV-1l originated from various geographical regions in different years. There was no correlation between genetic grouping and locations where isolates were obtained. Viruses originating from one farm in most cases belonged to the same subgroup (n=5). This study indicates that the newly detected subgroup BVDV-1l is predominant and widespread in Turkey. Moreover, an ovine virus isolate was identified as the first member of BVDV-2 reported in Turkey. A serological survey using samples from western Turkey indicated that BVDV-2 is also present in cattle. 相似文献
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Falcone E Cordioli P Tarantino M Muscillo M La Rosa G Tollis M 《Veterinary research communications》2003,27(6):485-494
The genetic characteristics, of 38 field isolates of bovine viral diarrhoea virus (BVDV) collected in 1999 from sick or healthy and persistently infected cattle of dairy farms situated in northern Italy, were investigated. A partial 5-untranslated region (5-UTR) sequence of each isolate was determined and a phylogenetic analysis was performed. All the isolates were classified as belonging to the BVDV-1 genotype and could be assigned to different BVDV-1 groups, namely BVDV-1b (n = 20), BVDV-1d (n = 6) and BVDV-1e (n = 10). Two remaining isolates could be classified as BVDV-1f and BVDV-1h, respectively. These results provided evidence for genetic heterogeneity of BVDV in Italy, and contribute to a better knowledge of the circulation of BVDV strains, and to their classification. 相似文献
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Llamas and alpacas are domesticated South American camelids (SACs) important to ancestral population in the Altiplano region, and to different communities worldwide where they have been introduced. These ungulates have shown to be susceptible to several livestock viral pathogens such as members of the Pestivirus genus, in particular Bovine Viral Diarrhea (BVDV), but there is little data available on Pestivirus infections in SACs. In this study we aimed to detect and identify Pestivirus genotypes and subgroups infecting SACs in both wild and confined environments. Samples were collected from 136 llamas and 30 alpacas from different areas in the Chilean Altiplano (wild animals), and from 22 llamas and 26 alpacas diagnosed as Pestivirus positive from the Metropolitana region in Chile (confined animals). Seroneutralization tests showed titers lower than 2 in all 166 samples from Chilean Altiplano. These samples were also negative to BVDV isolation, indicating that these animals have not been exposed to Pestivirus. After reactivation of positive samples from the Metropolitana region, the 5′ non-codifying region (5′NCR) and E2 glycoprotein were amplified by RT-PCR from the Pestivirus genome. Viral sequences were pairwise compared and phylogenetic trees were constructed. The 5′NCR analysis showed that all 12 sequenced isolates belonged to BVDV-1. Of particular interest, isolates from eight llama and two alpaca were BVDV-1j and two alpacas were BVDV-1b. In agreement with these results, E2 phylogenetic analysis rendered a similar grouping indicating that all 16 isolates belong to BVDV-1. However, the lower availability of E2 sequences determines the creation of a smaller number of sub-groups than the 5′NCR sequences. Based on the E2 sequences, the 5′NCR BVDV 1j group consisting of all the llamas and 3 alpacas are completely included in the E2 BVDV 1e group. Due to the universal availability of the 5′NCR segment, we propose the classification of these Chilean llamas and alpacas Pestivirus isolates as BVDV 1j and BVDV 1b respectively. Thus, this is the first time BVDV-1j is obtained in SACs. In addition, these results indicate Pestivirus infection in llamas and alpacas is associated with bovine population as genotypes and sub-groups are the same as those affecting Chilean livestock. 相似文献
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Tajima M 《The Japanese journal of veterinary research》2006,54(2-3):129-134
Genotypes and subgenotypes of bovine viral diarrhea virus (BVDV) field isolates from Japan, Germany and the United States of America (USA) were identified, and the prevalent pattern of BVDV in individual countries was estimated genetically. Subgenotypes were determined based on phylogenetic analyses of nucleotide sequences of a part of the E2-coding gene of BVDV. Forty-five, 61 and 56 BVDV strains were isolated from naturally infected cattle in Japan, Germany and USA, respectively, between 1980 and 2003. The most prevalent BVDV in these three countries was BVDV-1b. The second most prevalent BVDV strains were 1a, 1d and BVDV-2 in Japan, Germany and USA, respectively. The most prevalent subgenotype 1b in each country constructed individual small clusters in the subgenotype 1b branch in the phylogenetic tree. Although cattle and/or cattle products were moving among the three countries as part of international trade, the distribution of BVDV in the field in each country showed long-standing individual patterns. 相似文献
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Asami NISHIMORI Shizuka HIROSE Saho OGINO Kiyohiko ANDOH Norikazu ISODA Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):228
Bovine viral diarrhea virus (BVDV) is a causative agent of bovine viral diarrhea. In Japan, a previous study reported that subgenotype 1b viruses were predominant until 2014. Because there is little information regarding the recent epidemiological status of BVDV circulating in Japan, we performed genetic characterization of 909 BVDV isolates obtained between 2014 and 2020. We found that 657 and 252 isolates were classified as BVDV-1 and BVDV-2, respectively, and that they were further subdivided into 1a (35 isolates, 3.9%), 1b (588, 64.7%), 1c (34, 3.7%), and 2a (252, 27.7%). Phylogenetic analysis using entire E2 coding sequence revealed that a major domestic cluster in Japan among BVDV-1b and 2a viruses were unchanged from a previous study conducted from 2006 to 2014. These results provide updated information concerning the epidemic strain of BVDV in Japan, which would be helpful for appropriate vaccine selection. 相似文献
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Endsley JJ Quade MJ Terhaar B Roth JA 《Veterinary therapeutics : research in applied veterinary medicine》2002,3(4):364-372
Expression of CD25 (interleukin-2 receptor alpha chain) was used to monitor antigen-specific activation of T lymphocyte subsets (CD4+, CD8+, and gamma delta T cells) from cattle immunized with modified-live virus (MLV) bovine viral diarrhea virus (BVDV) vaccines. Two groups of 15 animals each were vaccinated with one dose of either BVDV genotype 1 (BVDV-1) or BVDV-1 and BVDV genotype 2 (BVDV-1/2). Six animals negative for both BVDV antibody and BVDV virus were used as negative controls. Three animals vaccinated 7 and 5 weeks before the start of the experiment with MLV BVDV-1 vaccine served as positive controls. Blood samples were taken from the negative control group, the positive control group, and the BVDV-1/2 group 0, 21, 35, 60, and 90 days after vaccination. Blood samples were taken from the BVDV-1 group 0, 21, and 90 days after vaccination. Isolated peripheral blood lymphocytes from immunized and control animals were incubated for 5 days with and without BVDV-1 or BVDV-2. Compared with nonvaccinated animals, a significant (P <.05) increase in expression of CD25 by CD4+ (60 days), CD8+, and gammadelta T (35 to 90 days) lymphocytes from the group given BVDV-1/2 was detected following in vitro exposure to BVDV-1 or BVDV-2 after vaccination. The CD8+ and gammadelta T cells from the group vaccinated with BVDV-1 had significantly (P <.05) increased expression of CD25 compared with nonvaccinates following postvaccination exposure to in vitro BVDV-1 but not to BVDV-2. There was no significant difference between the two vaccinated groups in CD25 expression on any of the T cell subsets in response to BVDV-1 or BVDV-2 exposure. A single administration of MLV BVDV vaccine may be more effective at stimulating CD8+ and gammadelta T cell-specific immune responses to the homologous genotype than to the heterologous genotype. 相似文献
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从牦牛(Yak)体内分离出牛病毒性腹泻/黏膜病病毒(BVDV/MDV),参考GenBank中已收录的BVDV-Ⅰ型的全基因组序列,设计了19对引物,通过RT-PCR方法,对牛病毒性腹泻病毒牦牛株进行克隆及测序,得到其全基因组序列(GenBank登录号:JQ799141),序列全长12214 nt,其中5'-UTR长288 nt,3'-UTR长232 nt。将牦牛株BVDV全基因序列与GenBank中登录的其他8株BVDV病毒全基因序列进行同源性比对及系统进化分析,结果表明,牦牛株BVDV与BVDV-Ⅰ型毒株出于同一分支,但与其他8株BVDV毒株全基因序列同源性均不高,有可能属于新的独立基因型或基因亚型,仍需进一步研究证实。 相似文献
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Maria P. Guelbenzu-Gonzalo Lynsey Cooper Craig Brown Sam Leinster Ronan O’Neill Liam Doyle David A. Graham 《Irish veterinary journal》2015,69(1):7
Background
The genus pestivirus within the family Flaviviridae includes bovine viral diarrhoea virus (BVDV) types 1 and 2, border disease virus (BDV) and classical swine fever virus. The two recognised genotypes of BVDV are divided into subtypes based on phylogenetic analysis, namely a-p for BVDV-1 and a-c for BVDV-2.Methods
Three studies were conducted to investigate the phylogenetic diversity of pestiviruses present in Northern Ireland. Firstly, pestiviruses in 152 serum samples that had previously tested positive for BVDV between 1999 and 2008 were genotyped with a RT-PCR assay. Secondly, the genetic heterogeneity of pestiviruses from 91 serum samples collected between 2008 and 2011 was investigated by phylogenetic analysis of a 288 base pair portion of the 5’ untranslated region (UTR). Finally, blood samples from 839 bovine and 4,437 ovine animals imported in 2010 and 2011 were tested for pestiviral RNA. Analysis of animal movement data alongside the phylogenetic analysis of the strains was carried out to identify any links between isolates and animal movement.Results
No BVDV-2 strains were detected. All of the 152 samples in the first study were genotyped as BVDV-1. Phylogenetic analysis indicated that the predominant subtype circulating was BVDV-1a (86 samples out of 91). The remaining five samples clustered close to reference strains in subtype BVDV-1b. Out of the imported animals, 18 bovine samples tested positive and 8 inconclusive (Ct ≥36), while all ovine samples were negative. Eight sequences were obtained and were defined as BVDV-1b. Analysis of movement data between herds failed to find links between herds where BVDV-1b was detected.Conclusion
Given that only BVDV-1a was detected in samples collected between 1968 and 1999, this study suggests that at least one new subtype has been introduced to Northern Ireland between 1999 and 2011 and highlights the potential for importation of cattle to introduce new strains.17.
1株猪源牛病毒性腹泻病毒的分离与鉴定 总被引：2，自引：1，他引：1
从BVDV阳性仔猪病料中分离病毒,为开展猪源BVDV病原学研究奠定基础。将处理后BVDV阳性仔猪组织样品接种MDBK细胞,分离到1株猪源BVDV,命名为SD0803株。通过细胞培养、直接免疫荧光、5′-UTR与Npro PCR扩增、电镜观察、TCID50测定及对其分子进化特征加以分析。结果表明,该毒株在MDBK细胞上盲传至13代未出现细胞病变。在直接免疫荧光试验中呈阳性荧光信号。PCR扩增分别获得5′-UTR与Npro预期大小DNA片段。电镜观察,病毒粒子略呈圆形,有囊膜,直径约50nm。病毒滴度为10-6.5 TCID50.0.2 mL-1。SD0803 5′-UTR、Npro序列进化分析显示,该分离株属于BVDV-1,与已知BVDV-1各亚型之间同源性较低,单独成一分支。结果表明,成功分离鉴定1株猪源BVDV SD0803,该毒株为非致病变型BVDV-1,极有可能为BVDV-1新的亚型。 相似文献
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Pestiviruses isolated from sheep and goats in India thus far have been bovine viral diarrhoea virus 1 (BVDV-1) or BVDV-2. During routine genetic typing of pestiviruses in the years 2009-10, border disease virus (BDV) was detected in eight Indian sheep of a flock showing clinical signs of BD by real time RT-PCR. All the samples yielded positive virus isolates in cell culture but were found negative by a BVDV antigen ELISA. A representative BDV isolate was characterized at genetic and antigenic level. Phylogenetic analysis carried out in 5′-UTR, Npro and E2 regions of genome typed the Indian BDV isolate as BDV-3. A more detailed analysis in Npro and entire region coding structural proteins showed that the Npro (168), C (100 aa), Erns (227 aa), E1 (195 aa) and E2 (373 aa) proteins were of size characteristic for BDV reference strain X818. Antigenic differences were evident between the BDV-3 isolate and previously reported BDV-1, BDV-5 and BDV-7 strains. Although origin of BDV-3 in India is not clear, the results reflect probable introduction through trade in sheep between India and other countries or BDV-3 may be more widely distributed. Additionally, this study suggests that for diagnosis of BDV infection, the commercial BVDV Ag-ELISA should be used with caution. This is the first identification of BDV in sheep in India which highlights the need for continued pestivirus surveillance and assessing its impact on sheep and goat production. 相似文献
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König M Cedillo Rosales S Becher P Thiel HJ 《Berliner und Münchener tier?rztliche Wochenschrift》2003,116(5-6):216-221
Pestiviruses cause economically important diseases of farm animals. Members of the Pestiviruses are bovine viral diarrhea virus 1 (BVDV-1), BVDV-2, classical swine fever virus (CSFV) and border disease virus (BDV). Phylogenetic analyses based on the entire nucleic acid sequence encoding the Npro allow a statistically significant segregation of established species and of subgroups within the species. BVDV-1 strains isolated in Germany can be associated with at least five different subgroups. In contrast all BVDV-2 isolates detected in Germany so far are closely related, belonging to one subgroup. A group of virus isolates from sheep and zoo animals is clearly different from established pestivirus species and can be designated as BDV-2. Antigenetic relatedness of pestiviruses was studied using defined virus isolates and antisera in cross-neutralization assays. Six antigenic groups were distinguished corresponding to the genetic clusters BVDV-1, BVDV-2, CSFV, BDV-1, BDV-2 and Giraffe-1. A significant antigenic difference was also observed between members of subgroups 1a and 1b of BVDV-1. Studies on the genetic and antigenic heterogeneity of pestiviruses are important for the development of new vaccines, diagnostic tests and for eradication programs. 相似文献
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Yuri ABE Tomokazu TAMURA Shiho TORII Shiho WAKAMORI Makoto NAGAI Kazuya MITSUHASHI Junki MINE Yuri FUJIMOTO Naofumi NAGASHIMA Fumi YOSHINO Yukihiko SUGITA Takushi NOMURA Masatoshi OKAMATSU Hiroshi KIDA Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):61-70
In our previous study, we genetically analyzed bovine viral diarrhea viruses (BVDVs)
isolated from 2000 to 2006 in Japan and reported that subgenotype 1b viruses were
predominant. In the present study, 766 BVDVs isolated from 2006 to 2014 in Hokkaido,
Japan, were genetically analyzed to understand recent epidemics. Phylogenetic analysis
based on nucleotide sequences of the 5′-untranslated region of viral genome revealed that
766 isolates were classified as genotype 1 (BVDV-1; 544 isolates) and genotype 2 (BVDV-2;
222). BVDV-1 isolates were further divided into BVDV-1a (93), 1b (371) and 1c (80)
subgenotypes, and all BVDV-2 isolates were grouped into BVDV-2a subgenotype (222). Further
comparative analysis was performed with BVDV-1a, 1b and 2a viruses isolated from 2001 to
2014. Phylogenetic analysis based on nucleotide sequences of the viral glycoprotein E2
gene, a major target of neutralizing antibodies, revealed that BVDV-1a, 1b and 2a isolates
were further classified into several clusters. Cross-neutralization tests showed that
BVDV-1b isolates were antigenically different from BVDV-1a isolates, and almost BVDV-1a,
1b and 2a isolates were antigenically similar among each subgenotype and each E2 cluster.
Taken together, BVDV-1b viruses are still predominant, and BVDV-2a viruses have increased
recently in Hokkaido, Japan. Field isolates of BVDV-1a, 1b and 2a show genetic diversity
on the E2 gene with antigenic conservation among each subgenotype during the last 14
years. 相似文献