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马士孝 《上海畜牧兽医通讯》2020,(4)
为了解南京市高淳区山羊弓形虫病流行情况,采用弓形虫抗体ELISA检测试剂盒对该地区多个山羊养殖场的642份样品进行弓形虫抗体检测。结果显示:642份样品中,68份血清样品检测为阳性,总阳性率达10.59%;不同年龄阶段羊群弓形虫病阳性率有较大差异,成年羊的弓形虫病阳性率(14.49%)明显高于未成年羔羊(8.64%)。成年羊中,以配种公羊阳性率最高,达16.22%;哺乳母羊次之,为14.61%;妊娠与空怀母羊最低,为13.64%。羔羊中,以断奶母羔羊弓形虫阳性率最高,为9.30%,哺乳羔羊阳性率最低,为7.87%,但两者差异不显著(P0.05)。综上可知,南京市高淳区存在一定程度的山羊弓形虫感染,需采取相关防控措施。 相似文献
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贵州省7个地区主要山羊流产疫病的流行病学调查 总被引：1，自引：0，他引：1
为了解2011—2012年间贵州省山羊5种流产疫病的流行情况,采用虎红平板凝集试验(RBPT)、间接血凝试验(IHA)和酶联免疫吸附试验(ELISA)3种血清学方法对贵州省7个地(州、市)74个养殖场的514份山羊血清进行5种流产疫病抗体的血清学监测,并通过PCR方法对10个规模化养殖场流产死亡母羊子宫、胎儿、肺和肺门淋巴结组织进行羊流产亲衣原体病和山羊传染性胸膜炎的病原核酸进行检测。结果显示:布氏杆菌病、羊流产亲衣原体病、弓形虫病、蓝舌病、山羊传染性胸膜炎的丝状支原体山羊亚种和绵羊肺炎支原体血清抗体阳性率分别为0、1.26%、6.72%、26.95%、2.35%和6.79%;羊流产亲衣原体病、山羊传染性胸膜肺炎的丝状支原体山羊亚种和绵羊肺炎支原体病原核酸检测阳性率分别为0、0、10%。结果表明:目前除布氏杆菌病以外,其余4种山羊流产疫病均在贵州存在不同程度的流行。 相似文献
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应用衣原体间接血凝试验(IHA),对青海省门源县山羊进行了衣原体血清抗体检测,共检测山羊血清303份,检出阳性48份,阳性率为15.84%。 相似文献
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为了解贵州省山羊流产与山羊痘的相关性,采用琼脂扩散试验和PCR法对本省10个市(县)流产羊群的血清和病料样本进行山羊痘抗原抗体及病原核酸检测,同时血清进行布氏杆菌抗体检测,流产胎儿病料进行羊流产亲衣原体病原核酸检测。结果发现山羊痘羊群流产率达37.1%(4329/11660),山羊痘血清抗体阳性率为38.2%(34/89),抗原阳性率为72.7%(32/44),流产胎儿山羊痘病毒核酸检出率为83.3%(10/12),发病羊群未检出布氏杆菌和羊流产亲衣原体感染。结果表明,山羊流产与山羊痘感染有一定关系,提示在山羊养殖中应加强饲养管理,防止山羊痘感染引起孕羊流产。 相似文献
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大通县家畜弓形虫病流行病学调查 总被引：3，自引：0，他引：3
陈才英 《青海畜牧兽医杂志》2008,38(1):23-23
用间接血凝试验(IHA)对大通县羊流产高发区家畜弓形虫病检测,结果表明,检测山羊血清1 028份,阳性268份,阳性率26.1%;山羊流产胎儿心血100份,阳性13份,阳性率13.0%;绵羊血清56份,阳性16份,阳性率28.6%;牦牛血清43份,阳性29份,阳性率67.4%;鸡血清66份,阳性7份,阳性率10.6%;猪血清80份,阳性11份,阳性率13.8%;马血清149份,阳性4份,阳性率2.7%. 相似文献
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Tania de Freitas Raso Angelo Berchieri Júnior Aramis Augusto Pinto 《Journal of zoo and wildlife medicine》2002,33(2):118-121
The prevalence of Chlamydophila psittaci (formerly Chlamydia psittaci) infection was assessed in 95 apparently healthy, captive Amazon parrots from three breeder collections in southeastern and west-central Brazil. Cloacal swabs from 95 birds were tested for chlamydial antigen, which was detected by direct immunofluorescence (DIF), and serum samples from 44 of these birds were tested for antibodies to C. psittaci using an enzyme-linked immunosorbent assay. The prevalences of active infection as detected by DIF were 16.7%, 22.2%, and 56.1%, and seroprevalences were 100%, 87.5%, and 60% in flocks A, B, and C, respectively. We can therefore infer that C. psittaci may be widespread in captive parrot populations in Brazil. 相似文献
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J Apel O J Hübschle H Krauss 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1989,36(6):447-458
An IgG (H+L)-ELISA was applied as a screening test for antibodies against Chlamydia (C.) psittaci in sera of goats and sheep in Namibia. In 576 (27.3%) of a total of 2,107 sera (299 = 25.2% of 1,185 caprine and 277 = 30.0% of 922 ovine sera) chlamydial antibodies could be detected. 86% of all farms tested revealed seropositive animals. Chlamydial infections were prevalent in all the geographical regions tested. The infection rates per State Veterinary District varied from 12.0% (Otjiwarongo) to 50.0% (Otavi) in goats and from 13.3% (Otjiwarongo) to 41.7% (Windhoek) in sheep. The regional distribution of chlamydial infections was not related to geographical or climatic factors. Sera from herds showing symptoms indicative for chlamydial infections showed significantly higher antibody rates (35% in goats and 39% in sheep) than sera from herds without health problems (18% in goats and 24% in sheep). Considering only sera from farms with clinical history of chlamydiosis, high seroprevalences were correlated to the symptoms abortion and keratoconjunctivitis. As in other countries, enzootic abortion seems to be the main manifestation of chlamydial infection in small ruminants in Namibia. C. psittaci might also play a considerable role in the etiology of infectious keratoconjunctivitis, whereas association with other clinical entities seems to be rare. 相似文献
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Sharma SP Baipoledi EK Nyange JF Tlagae L 《The Onderstepoort journal of veterinary research》2003,70(1):65-68
The prevalence of antibodies to Toxoplasma gondii and Chlamydia psittaci was assessed in goats with a history of abortion, stillbirth and neonatal mortality. Antibodies were detected in 540 (30%) and 57 (3.2%) goats out of 1799 tested by indirect haemagglutination and complement fixation tests, respectively. Toxoplasma gondii was isolated for the first time in Botswana from 22 out of 81 sets (27.2%) of foetal tissues, maternal and foetal cotyledons and uterine tissues of goats which had previously aborted or given birth to stillborn or weak kids that died within two days of birth. These results implicate T. gondii and C. psittaci, but especially the former, to be associated with caprine reproductive problems and require appropriate control measures. 相似文献
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青海省海西州改良绒山羊衣原体病血清学诊断 总被引：1，自引：0，他引：1
李晓卉 《青海畜牧兽医杂志》2008,38(1):16-17
采集青海省海西州部分地区改良绒山羊血清样品561份,用间接血凝试验检测衣原体抗体.结果检出阳性123份,平均阳性检出率为21.93%. 相似文献
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Analysis of caprine IgG1 and IgG2 subclass responses to Chlamydia psittaci infection and vaccination
N Schmeer H Krauss J Apel M Adami H P Müller W Schneider J A Perez-Martinez H Rieser 《Veterinary microbiology》1987,14(2):125-135
Enzyme-linked immunosorbent assays (ELISAs) specific for caprine IgG(H+L), IgG1 and IgG2 were developed and evaluated for serodiagnosis of Chlamydia psittaci infections in a Tunisian goat flock with currently occurring chlamydial abortions and a clinically inapparent goat flock of an animal research facility. Additionally, ELISAs were applied to record the IgG1 and IgG2 dynamics of four goats vaccinated with inactivated Chlamydia psittaci and Coxiella burnetii. For screening purposes, the IgG(H+L) ELISA proved to be superior to the complement fixation test because it detected a larger number of chlamydial abortions and was easier to perform and to interpret. Analysis of Chlamydia psittaci-specific IgG1 and IgG2 responses to naturally occurring infections by ELISA revealed high IgG1 levels associated with IgG2 in goats with current abortions, whereas clinically inapparent, but seropositive goats were characterized by significantly lower IgG1 levels only (P less than 0.001). Similarly, the four vaccinated goats responded initially with Chlamydia psittaci-specific IgG1, whereas second and third vaccinations induced (as in goats with chlamydial abortions) predominantly IgG1, but also IgG2. The results indicated that clinically inapparent chlamydial infection may be distinguished from overt disease by analysing specific IgG1 and IgG2 responses. Applying Coxiella burnetii- specific ELISAs on field samples, IgG1 alone could be detected in eight, IgG2 alone in one and IgG1 combined with IgG2 in nine goats. The coxiella-specific antibody response of the four vaccinated goats was--in contrast to the chlamydia-specific response--characterized by IgG2 dominance. 相似文献
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猪流行性乙型脑炎的血清学调查 总被引：4，自引：1，他引：3
为调查贵州省猪乙型脑炎病毒（Japanese encephalitis virus, JEV）的隐性感染率和使用疫苗后的血清抗体水平,采用间接酶联免疫吸附试验（ELISA）对贵州省规模化养猪场和农村散养户共764头份猪血清样本进行了JEV抗体水平检测。结果表明,未免疫猪群JEV的血清抗体阳性率为13.25%;而免疫猪群中JEV的血清抗体阳性率为82.18%;表明JEV的免疫效果比较理想,但未免疫猪群存在JEV感染,应引起重视。 相似文献
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Nineteen ewes were injected subcutaneously with the agent of enzootic ovine abortion, Chlamydia psittaci serovar 1, at 50 days gestation. Placental and fetal tissues were examined at 15 days postinfection and thereafter at ten day intervals. Placental infection was detected at 15 days postinfection. Only postinoculation sera collected from postinfected ewes contained antibodies reactive to C. psittaci. Five (26%) chlamydial infected ewes experienced inapparent fetal loss before day 105 of gestation. This finding is significant since C. psittaci infection in sheep is commonly associated with abortion and not infertility. 相似文献