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1.
多肽在荔枝上的应用试验 总被引：4，自引：0，他引：4
为探求多肽在荔枝上的应用效果,以妃子笑和南岛无核品种为试材进行了应用试验.结果表明:施用多肽对成花、坐果、产量和果实品质均有明显的促进作用,与CK相比,妃子笑和南岛无核荔枝单株花穗数分别增加15.76%和20.60%,单穗雌花数分别增加40.59%和28.90%,单果重分别增加4.95%和6.49%,株产分别增加9.48%和12.10%,可溶性固形物含量分别增加7.14%和12.12%,Vc含量分别增加217.64%和202.05%,可滴定酸含量分别减少17.68%和18.18%,差异均极显著. 相似文献
2.
以新疆阿克苏地区水心（冰糖心）富士苹果为试材,分析讨论了果实碳水化合物、矿质元素和活性氧代谢与水心发生的关系。结果表明：果实可溶性固形物（SSC）含量越高,水心指数越高,呈显著正相关（P < 0.05）;而单果质量、果实纵横径比及L、b*、h°值与水心果率和水心指数相关不显著;进一步分析发现,与正常果实和组织相比,水心果实和组织中,山梨醇和蔗糖含量均显著升高,尤其是山梨醇含量,分别是正常果实和组织的2.19倍和2.86倍,果糖和葡萄糖含量相对较低;此外,水心组织中K和B含量显著高于正常组织,而Ca、Zn及Fe含量则显著降低;同时,水心果实和组织中超氧阴离子和过氧化氢含量较正常果实和组织显著升高,分别高282.39%、68.17%和12.6%、107.62%;POD、SOD和CAT等抗氧化酶活性显著降低,MDA含量和相对电导率显著升高,膜质过氧化水平严重。 相似文献
3.
硫和钴在杨梅植株体内的分布及对生长的影响 总被引：3，自引：0，他引：3
杨梅对硫有较强的吸收能力, 3 年生‘东魁’杨梅定植一年后, 在未施任何肥料的情况下,使土壤含硫量下降了37.7 %。杨梅吸收硫主要积累在叶片和根瘤中, 叶片和根瘤中的含硫量是根的2 倍左右。杨梅吸收钴主要积累在根瘤和根中, 根瘤和根中钴含量是叶片的4.88 和4.48 倍。每株施硫酸钴15 和30 mg 能促进杨梅对硫和钴的吸收利用, 且硫和钴优先向根瘤运输和积累; 根瘤和叶片含硫量分别是根系含硫量的3.38～4.58 倍和2.75～3.72 倍, 根瘤含硫量又是叶片含硫量的1.23 倍; 根瘤含钴量分别是根和叶的1.39～1.90 倍和3.53～4.55 倍。每株施硫酸钴15 和30 mg , 能显著增加杨梅植株的生物量、株高、根瘤量和固氮酶活性, 且根瘤的固氮酶活性是对照的8～18.35 倍。提出硫元素与Frankia 放线菌的共生固氮有关, 有利于根瘤形成和固氮。 相似文献
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5.
毛花猕猴桃‘华特’果实采后糖代谢研究 总被引：1，自引：0，他引：1
《果树学报》2016,(6)
【目的】探究毛花猕猴桃果实采后在常温和低温贮藏下果肉与果心的糖代谢特性。【方法】以毛花猕猴桃新品种‘华特’为材料,将采后果实分别在常温(20℃)和低温(1℃)贮藏28 d和56 d,检测果肉和果心的淀粉、蔗糖、果糖和葡萄糖含量、淀粉酶和蔗糖代谢相关酶活性的变化。【结果】‘华特’果肉和果心均以积累己糖为主,两者总糖含量相近,但不同糖的分配存在差异,特别是果心的蔗糖含量极显著高于果肉的含量。果肉和果心的淀粉和糖含量的变化趋势基本一致,即淀粉伴随着淀粉酶活性的升高迅速降解,果糖和葡萄糖快速积累,而蔗糖含量逐渐下降。果肉和果心糖含量的差异变化是蔗糖代谢各种酶协同作用的结果。果肉和果心的蔗糖分解酶活性,即酸性转化酶(AI)和中性转化酶(NI)活性高于蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)活性,使蔗糖含量逐渐下降。【结论】‘华特’果实采后果肉与果心的蔗糖、果糖和葡萄糖含量变化表现差异,果肉具有较高AI和NI活性,及较低SPS活性是导致其蔗糖含量显著低于果心的重要原因。低温贮藏有效降低了果实淀粉酶、AI、NI、SPS和SS活性的变化幅度,从而缓解了果肉和果心蔗糖和淀粉的降解,也延缓了果糖和葡萄糖的积累。 相似文献
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<正>受气候和栽培管理等因素影响,2009年西安葡萄产区霜霉病的发生出现了一些新情况:一是发病早,从往年的6月份提前到4月下旬暴发;二是侵染快,危害重,发生后迅速蔓延,不但在叶片和嫩梢上发生,而且侵染到花序和果穗,导致大量花序和幼果期果穗发病,有些直接造成穗轴和果梗干枯,不仅直接影响葡萄品质和产量,而且 相似文献
7.
本文以龙柏和国槐作为行道树木修剪的研究对象,努力探索能够和整体建筑相匹配,适合公园园林发展需求的大型行道树木的修剪方法,提升人文和整体景观效果。作为行道树,受环境因素和空间的影响,龙柏的修剪要保持其结构的完善和挺拔的树形,采取底部规整上部自然的修剪方法,使得植物在自然美的基础上,与建筑相得益彰,烘托出整体的园林氛围。国槐为大型落叶乔木,可根据周围具体空间情况和植物生长的主次关系以及人工管理的需求和安全性出发,进行适当的修剪,主要对上部生长过于旺盛和底部旁逸斜出的枝条进行短截或者回缩,抑制整体树势高度和冠幅不断扩大,保持整体树形紧凑,达到植物总体生长与管理的和谐统一。 相似文献
8.
腐植酸液肥对油麦菜和生菜产量及品质的影响 总被引：1，自引：0，他引：1
以油麦菜和生菜为试材,采用随机区组田间试验,在施用等量基肥情况下,研究追施腐植酸液肥对油麦菜和生菜产量及品质的影响.结果表明:与复合肥追肥相比,腐植酸液肥处理的油麦菜和生菜产量分别增加4.0％和6.4％,油麦菜的水溶性氨基酸、可溶性糖和可溶性蛋白质的含量分别提高44.58％、14.03％和9.27％,硝酸盐含量降低8.36％;生菜的水溶性氨基酸、可溶性糖和可溶性蛋白质的含量分别提高17.36％、11.04％和18.81％,硝酸盐含量降低6.89％.表明追施腐植酸液肥具有增产效果,并能有效改善油麦菜和生菜品质. 相似文献
9.
《中国瓜菜》2019,(9):34-37
以大田菠菜为试验材料,采用1.0 mg·L~(-1)臭氧水土壤浇灌和0.6 mg·L~(-1)臭氧水叶片喷施处理,测定菠菜幼苗相关生理指标和营养成分。结果表明,臭氧水浇灌和喷施处理显著增加菠菜幼苗鲜质量,分别比对照增加了80.07%和14.49%,明显促进菠菜幼苗生长;显著降低菠菜叶片质膜透性,分别比对照降低了53.85%和46.15%,提高了菠菜幼苗的抗逆能力;显著增加菠菜叶片氮素含量、SPAD值和叶绿素含量,浇灌处理分别比对照增加了30.74%,54.17%和125.32%,喷施处理分别比对照增加了12.45%,26.28%和89.87%,明显提高了菠菜幼苗的光合能力;显著增加菠菜叶片Fe、B和Mn含量及维生素C和可溶性糖含量,浇灌处理分别比对照增加了62.69%,30.08%,70.48%,22.58%和100.69%,喷施处理分别比对照增加了52.24%,21.59%,20.75%,9.68%和29.95%,明显改善了菠菜幼苗生理特性,提高了产量和品质。与叶片喷施处理相比,臭氧水浇灌处理的效果更好。 相似文献
10.
张放 《柑桔与亚热带果树信息》2012,(4):30-36
柑桔是我国仅次于苹果的第二大出口水果。2011年我国柑桔在出口量持续下降的同时,进口量呈继续增长态势。其中,出口量和出口额分别为90.16万t和72646万美元,与2010年相比,出口量减少了3.38%,出口额增加了17.97%,分别占2011年我国干鲜水果出口总量和总额的28.87%和22.78%;进口量和进口额分别为13.17万t和14834万美元,与2010年相比,分别增加了25.14%和39.87%,分别占2011年我国干鲜水果进口总量和总额的4.08%和4.91%。我国柑桔绝大部分出口邻近的东盟及独联体国家和地区,越南是出口量最大的目标市场,印度尼西亚是出口额最大的目标市场。宽皮柑桔是我国出口量最大的的柑桔水果,2011年出口量小幅增长,达70.87万t;而其他类型的柑桔水果出口量均有不同幅度减少,其中,甜橙和柠檬与酸橙出口量下降幅度近40%。 相似文献
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AIM: To investigate the combination therapeutic effect of ulinastatin and berberine on burn sepsis in the rats.METHODS: The rats with burn sepsis were administrated with ulinastatin or berberine or combination therapy. The survival rate and body weight of the rats were measured. The levels of IL-6, IL-10 and TNF-α were examined by ELISA. The morphological changes of the lung were observed by hematoxylin and eosin staining. The content of bacteria in the liver, lung and spleen tissues was detected.RESULTS: Combination of ulinastatin and berberine significantly increased the survival rate and body weight in the rats with burn sepsis. Moreover, combination therapy inhibited the elevation of IL-6 and TNF-α levels, whereas increased the IL-10 level. Combination therapy protected the structure of hepatic lobule, and further reduced the bacterial content in the liver, lung and spleen. CONCLUSION: Combination of ulinastatin and berberine improves the symptoms of burn sepsis by regulating the levels of IL-6, IL-10 and TNF-α and inhibiting the content of bacteria. 相似文献
12.
LI Zhong-hong SHU Zhao-hui LIAO Ying-qin LIU Pei-qing LU Jing WANG Ping WANG Gui-xiang PAN Xue-diao LAN Tian ZANG Lin-quan ZHOU Si-gui 《园艺学报》2018,34(2):251-257
AIM: To investigate the changes of short-chain acyl-CoA dehydrogenase (SCAD) in hypertensive vascular remodeling and to explore the relationship between SCAD and vascular remodeling in hypertension.METHODS: The spontaneously hypertensive rats (SHR; 24 weeks old) and Wistar rats (24 weeks old) were used as experimental control groups. The SHR and Wistar rats of 16 weeks old were trained by swimming as experimental groups. The systolic pressure was measured periodically. The thickness of vascular wall and the diameter of the vascular lumen were measured. The contents of ROS and ATP, the enzyme activity of SCAD, and the expression of SCAD at mRNA and protein levels in the aorta were determined. The free fatty acid in the serum and aorta was also measured.RESULTS: Compared with Wistar group, the diameter of vascular lumen decreased in SHR group. The thickness of vascular wall, the ratio of vascular wall and the diameter of vascular lumen, and the blood pressure in SHR group were increased significantly (P<0.05). Compared with SHR group, the diameter of vascular lumen increased in SHR+swim group. The thickness of vascular wall, the ratio of vascular wall and the diameter of vascular lumen, and the blood pressure in SHR+swim group were decreased significantly. Compared with control group, the expression of SCAD at mRNA and protein levels, the enzyme activity of SCAD, and the content of ATP were decreased in SHR group. However, the free fatty acid in the serum and aorta, and the content of ROS in the aorta were increased in SHR group. The expression of SCAD at mRNA and protein levels, the enzyme activity of SCAD, the content of ATP were increased in Wistar+swim group and SHR+swim group. However, the free fatty acid in serum and aorta, and the content of ROS in the aorta were decreased in Wistar+swim group and SHR+swim group.CONCLUSION: Decrease in SCAD expression may be associated with hypertensive vascular remodeling. Swimming training can reverse hypertensive vascular remodeling by increasing the expression of SCAD in the aorta. 相似文献
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蔗糖代谢相关酶在卡因菠萝果实糖积累中的作用 总被引：12，自引：1，他引：12
以菠萝品种卡因为材料,测定了不同发育时期果实中蔗糖、葡萄糖和果糖含量以及蔗糖代谢相关酶-酸性转化酶(AI)、中性转化酶(NI)、蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)的活性,并对果实中糖积累与酶活性的关系进行了分析.结果表明,卡因果实的发育呈单"S型"曲线,在幼果期到果实迅速生长期,果糖和葡萄糖积累较多,蔗糖积累缓慢,此期蔗糖积累较少与高活性蔗糖转化酶有关;进入成熟期,果实中蔗糖迅速积累,而己糖略有上升,果实蔗糖的显著增加主要与SS和SPS活性有关.酸性转化酶和中性转化酶前期活性很强,后期降低;蔗糖合成酶(合成方向)和蔗糖磷酸合成酶的活性变化趋势相似,前期弱后期强.经过相关性分析:果实中蔗糖含量与SS和SPS活性存在极显著的正相关性(相关系数分别为r=0.97273**和r=-0.91004**),而与NI活性呈极显著负相关(r=-0.76419**),与AI的活性呈显著负相关(r=-0.60594*). 相似文献
14.
南丰蜜橘果园生草栽培对土壤团聚体和有机碳特征及果实品质的影响 总被引：4，自引：0，他引：4
以‘南丰蜜橘’果园土壤为对象,分析行间播种白三叶草（Trifolium repens）、黑麦草（Lolium multiflorum）和清耕条件下土壤机械稳定性团聚体和水稳性团聚体组成、团聚体稳定性、团聚体有机碳含量及其贡献率的差异。结果表明：生草后土壤机械稳定性团聚体以 > 5 mm粒级团聚体居多（占比28.52% ~ 35.14%）,而清耕对照处理以 < 0.25 mm粒级团聚体居多（35.51% ~ 36.86%）;水稳性团聚体各处理均以 < 0.25 mm粒级团聚体为多（55.71% ~ 80.47%）;大团聚体比重R0.25总体为黑麦草 > 白三叶草 > 对照。在0 ~ 10 和10 ~ 20 cm土层中,白三叶草处理机械稳定性团聚体和水稳性团聚体平均质量直径（MWD）较对照分别提高了126.03%、106.8%和57.69%、31.25%,团聚体破坏率（PAD）分别降低了13.29%和12.41%;黑麦草处理机械稳定性团聚体和水稳性团聚体平均质量直径（MWD）分别提高了140.41%、125.17%和82.69%、64.58%,PAD分别降低了12.85%和16.61%,表明生草处理有利于大团聚体的形成,改善土壤结构的稳定性,且禾本科类牧草黑麦草处理明显优于豆科类牧草白三叶草处理。生草处理较大幅度提高了0 ~ 10 cm表层土层水稳性团聚体中总有机碳和各粒级团聚体有机碳含量,且白三叶草处理明显优于黑麦草处理。相关性分析表明,团聚体MWD和GMD与1 ~ 5 mm粒级团聚体有机碳含量之间均呈现显著正相关,PAD与各粒级团聚体有机碳含量之间均呈现出负相关。生草处理能明显提高‘南丰蜜橘’果实可溶性糖、可溶性固形物和维生素C含量,降低可滴定酸含量,改善果实鲜食品质,且白三叶草处理明显优于黑麦草处理。 相似文献
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桂花（Osmanthus fragrans Lour.）野生资源有非常丰富的性状和遗传变异,但目前被过度开发、破坏,导致分布范围急剧缩小,种群规模和数量锐减,生境片断化严重。以往的文献认为桂花起源于喜马拉雅山脉东段,即中国的西南地区;然而随着野生种群在湖南、浙江和福建等地被陆续发现,关于桂花自然分布的问题需要重新审视。作者在大量野外调研的基础上,结合前人研究结果,对桂花的自然分布和种群特征等进行了总结和概括,分析了野生资源与品种起源的关系,以期为桂花野生资源的有效保护和高效利用提供参考。 相似文献
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为探明番茄嫁接后砧木、接穗残株再生性及再利用的可行性,以番茄砧木品种久绿787和接穗品种佳红4号为试材,研究砧木、接穗残株再生苗的生长发育进程,比较再生苗与初次嫁接苗的愈合情况和生长指标。结果表明:番茄嫁接后砧木残株可扦插再生、接穗残株可腋芽再生形成完整的幼苗,与播种形成幼苗相比,砧木残株扦插苗和接穗残株腋芽再生苗达到嫁接苗龄的时间可缩短16 d;利用砧木、接穗残株嫁接,嫁接苗成活率、接合力、输导能力及后期生长参数与初次嫁接苗差异不显著。通过嫁接苗培养成本比较,利用砧/穗残株进行嫁接的幼苗生产成本明显低于初次嫁接苗,按每万株嫁接苗计,可节约成本875.5元。说明番茄嫁接后砧/穗残株可以嫁接再利用,并可明显提高经济效益。 相似文献
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AIM: To investigate the effect and its molecular mechanism of microRNA-137(miR-137) on the invasion, migration abilities and apoptosis of breast cancer cells. METHODS: miR-137 mimimics were transfected into the breast cancer MDA-MB-231 cells. The expression of miR-137 was detected by RT-qPCR. Apoptosis was analyzed by flow cytometry. The invasion and migration abilities were detected by Transwell assays. The protein levels of matrix metalloproteinase 9 (MMP-9), cleaved caspase-3 (C-caspase-3) and Bax were determined by Western blot. Bioinformatics software was used to predict that TWIST1 might be the target gene of miR-137 and then it was conformed by luciferase reporter gene identification. The effect of miR-137 mimics on TWIST1 protein expression was evaluated by Western blot. TWIST1 over-expression vector and miR-137 mimics were co-transfected into the MDA-MB-231 cells, and then the apoptosis, invasion, migration abilities and the protein levels of MMP-9, C-caspase-3 and Bax were determined. RESULTS: In the miR-137 mimics transfected MDA-MB-231 cells, the expression level of miR-137 and the apoptosis rate were increased, the cell invasion and migration abilities were decreased, the protein levels of C-caspase-3 and Bax were increased, the protein expression of MMP-9 was decreased (P<0.05). In addition, the target regulation of TWIST1 by miR-137 was identified by luciferase reporter assay. Moreover, the expression of TWIST1 in the MDA-MB-231 cells was inhibited by miR-137 mimics. Compared with the MDA-MB-231 cells co-transfected with negative control vector and miR-137 mimics, the protein expression levels of TWIST1 and MMP-9 in the MDA-MB-231 cells co-transfected with TWIST1 over-expression vector and miR-137 mimics were increased, the protein levels of C-caspase-3 and Bax and the apoptosis rate were decreased, the cell invasion and migration abilities were increased. CONCLUSION: miR-137 inhibits the invasion, migration abilities and induces apoptosis of breast cancer cells through targeting TWIST1. 相似文献
18.
AIM: To investigate the effects of monopolar electrocoagulation on the changes of electrolytes and uterine ultrastructure in the patients with laparoscopically hysterectomy.METHODS: Sixty patients, who accepted laparoscopically hysterectomy in the Third Affiliated Hospital of Jinan University from December 2009 to December 2010,were selected in the study and divided into 2 groups by the different hemostasis methods.Thirty patients were in monopolar electrocoagulation group and another 30 patients were in Ligasure group.the plasma concentrations of electrolytes (potassium, sodium, chlorine and calcium) were measured before operation, during operation, 24 h and 48 h after operation.The uteruses from 5 patients in each group were collected and the dissections between uterine horn and fallopian tube were prepared for observations under light microscope and transmission electron microscope.RESULTS: Compared to the levels before operation, the concentrations of plasm potassium in all patients were increased during operation,and the plasm sodium was not changed due to the supplement during and after operation, but the plasm chlorine and calcium were decreased.The electrolyte levels came to normal 24 h and 48 h after operation.Comparing the difference between 2 groups, no statistical significance of electrolyte concentrations was observed.Under transmission electron microscope, breakage of the cell membrane, expansion of mitochondria, pyknosis of chromation and karyorrhexis were also observed.CONCLUSION: The pathological changes exist under transmission electron microscope, although no change of the tissues in the place 10 mm far away from monopolar electrocoagulation is found under light microscope. 相似文献
19.
甘蔗渣培养基栽培木耳过程中主要组分的降解和有关酶活的变化 总被引：1，自引：0，他引：1
实验以甘蔗渣加少量麦麸栽培毛木耳和光木耳,在栽培的不同阶段分析测定培养物的的主要化学组成成分和有关酶活性的变化。结果表明,两种木耳利用栽培底物的能力有明显差别;在栽培后期,毛木耳和光木耳分别消耗纤维素为74%和43%,半纤维素为78%和61%,木质素寿60%和35%。不过它们对这些底物的消耗又具有相似的阶段性变化的特点,即在子实体形成期的消耗速率都比菌丝生长期快。培养物的纤维素酶和半纤维素酶的活性也有阶段性变化特点。底物降解速率的变化与酶活水平的变化之间有一定对应关系,其中尤以毛木耳较明显。实验还分析培养物的过氧化物酶和多酚氧化酶的活性变化,简要地讨论了它们在栽培底物降解中的作用。 相似文献
20.
山东寿光半地下式日光温室主要生态因子变化特征 总被引：2，自引：0，他引：2
2009年9月～2010年5月对山东寿光地区半地下式和地平式日光温室主要生态因子进行测定。结果表明：晴天条件下半地下式日光温室内昼夜气温变化范围为14.8～40.6 ℃,地温19.0～25.0 ℃,平均值1月最低,5月最高;空气相对湿度42.6 %～97.7 %,平均值1月和3月较高,5月最低。不同时期气温和地温的昼夜变化规律相似,气温每天5：30～7：30最低,12：00～13：30最高;地温的最低值和最高值分别出现在8：30～10：30和17：30～20：00。相对湿度的昼夜变化趋势与气温相反,12：00～15：00最低,7：00～9：00最高。测定期内半地下式日光温室白天的光量子通量密度变化范围为211.0～1 304.7 μmol?m-2?s-1,CO2浓度为207～1 219 μl?L-1。晴天光量子通量密度先升高后降低,而CO2浓度则先降低后升高,峰值和谷值出现在11：00～14：00。与地平式相比,半地下式日光温室的气温、地温、相对湿度较高,光量子通量密度和CO2浓度差异不大。一天中气温高于35 ℃和相对湿度高于90 %的时间较长,气温低于20 ℃和相对湿度低于70 %的时间较短。 相似文献