共查询到20条相似文献，搜索用时 171 毫秒
1.
芦荟乙酰化甘露聚糖对肉仔鸡肠道主要菌群、小肠微绒毛密度、免疫功能及生产性能的影响 总被引：1，自引：0，他引：1
为研究芦荟凝胶、芦荟多糖、乙酰化甘露聚糖对内仔鸡肠道主要菌群、小肠微绒毛密度、免疫功能及生产性能的影响，600只粤黄鸡随机分为5组．每组设6个重复．雌雄各半。分别施以5种不同日粮：①基础日粮添加乙酰化甘露聚糖0．1％；②基础日粮添加乙酰化甘露聚糖0．05％；③基础日粮添加芦荟多糖0．1％；④基础日粮添加芦荟凝胶干粉0.1％；⑤对照组（基础日粮），试验期为56d。结果表明．肉仔鸡饲粮中添加芦荟凝胶、芦荟多糖、乙酰化甘露聚糖。能显著降低盲肠内容物大肠杆菌浓度．提高盲肠内双歧杆菌和乳酸菌杆菌浓度（P〈0．05）；回肠微绒毛高度增加、密度加大（P〈0．05）；免疫器官胸腺、脾脏、法氏囊相对质量均显著增加．新城疫抗体效价显著提高（P〈0．05）；添加芦荟多糖提高了肉仔鸡的日增重和降低了料肉比．但试验组与对照组之间的差异不显著。 相似文献
2.
研究大肠杆菌热敏性肠毒素突变体蛋白(heat-labile enterotoxin,LTRG)对鸡新城疫(Newcastle disease virus,NDV)疫苗经不同途径接种效果的影响.以NDV LaSota株作为共免疫原,与LTRG重组蛋白经滴鼻、口服及肌肉注射三种途径接种雏鸡,通过血清IgG抗体、HI抗体及粘膜分泌型IgA抗体水平变化,评价不同途径接种LTRG对NDV疫苗的免疫效果的影响.结果显示:①三种途径接种,LTRG均能显著增强NDV疫苗的免疫抗体水平,免疫雏鸡血清抗体和粘膜抗体水平均高于NDV疫苗单独免疫组;②LTRG对NDV疫苗免疫增强作用,以滴鼻接种最显著:二、三免后LTRG+NDV滴鼻组与NDV组血清IgG、粘膜IgA差异均显著(P＜0.01、P＜0.05);LTRG+NDV口服与NDV组血清IgG差异不显著(P＞0.05)、粘膜IgA差异板显著(P＜0.01);LTRG+NDV肌注与NDV组IgG差异不显著(P＞0.05)、粘膜IgA差异显著(P＜0.05);③三种免疫途径比较:血清IgG抗体水平差异均不明显(P＞0.05);滴鼻、口服免疫可诱导雏鸡产生较高的粘膜IgA抗体、血清HI抗体.由此可见:LTRG粘膜佐剂对NDV疫苗免疫增强作用,以粘膜(滴鼻、口服)途径接种效果最为显著,能诱导高水平的粘膜IgA抗体及血清抗体. 相似文献
3.
旨在探讨饲粮中添加黑沙蒿醇提物(AOEE)对肉仔鸡生长性能、免疫指标及相关基因表达量的影响。试验选取240只体质量相近、健康1日龄AA肉仔鸡,随机分为5个处理组,每组6个重复,每个重复8只鸡。对照组饲喂基础饲粮,试验组在基础饲粮中分别添加250,500,750和1 000 mg/kg AOEE,试验期为42 d。结果表明：42日龄时,随着AOEE添加量的增加,肉仔鸡的平均体质量呈极显著的一次线性或二次曲线升高效应(P<0.01),且与对照组相比,饲粮中添加750和1 000 mg/kg AOEE对肉仔鸡效果显著(P<0.05);AOEE以剂量依赖的方式显著提高了肉仔鸡前期、后期以及全期的饲料转化率(P<0.05)。42日龄时,随着AOEE添加量的增加,肉仔鸡脾脏指数与胸腺指数呈显著的一次线性或二次曲线升高效应(P<0.05)。21日龄时,随着AOEE添加量的增加,肉仔鸡肝脏IgA含量呈显著的一次线性或二次曲线升高效应(P<0.05),且与对照组相比,饲粮中添加500,750,1 000 mg/kg AOEE显著提高肉仔鸡肝脏、脾脏中IgG和IgM的含量(P... 相似文献
4.
中草药和芦荟多糖对肉仔鸡肠道微生态、免疫功能及生产性能的影响 总被引：2，自引：1，他引：2
研究了中草药添加剂、芦荟多糖对肉仔鸡肠道主要菌群、小肠微绒毛密度、免疫功能及生产性能的影响.结果表明,肉仔鸡饲粮中添加中草药、芦荟多糖,能显著降低盲肠内容物大肠杆菌浓度,提高盲肠内双歧杆菌和乳酸菌杆菌浓度(P＜0.05);回肠微绒毛高度增加、密度加大(P＜0.05);免疫器官胸腺、脾脏、法氏囊相对质量均显著增加,新城疫抗体效价显著提高(P＜0.05);添加中草药、中草药 芦荟多糖显著提高了肉仔鸡的日增重和降低了料肉比(P＜0.05). 相似文献
5.
为研究甘露寡糖脂质体纳米制剂对鸡生长发育及免疫功能的影响,在饲料中添加不同剂量的甘露寡糖脂质体纳米制剂(100、50、25mL g/kg),饲养49d后,测定了鸡体重、料肉比、免疫指标及血液中免疫球蛋白G(IgG)含量。结果表明,甘露寡糖脂质体纳米制剂能提高正常鸡的平均日增重,降低料肉比,其中在1日龄～21日龄阶段,高剂量组和低剂量组平均日增重明显高于药物对照组,差异极显著(P<0.01),与空白对照组比较,差异显著(P<0.05);甘露寡糖脂质体纳米制剂高、中、低剂量组鸡血浆中一氧化氮含量,与药物对照组、空白对照组比较均极显著升高(P<0.01)。说明甘露寡糖脂质体纳米制剂对鸡的免疫功能具有一定的调节作用,发挥抗病促生长作用。 相似文献
6.
试验选择1日龄AA肉仔鸡144只,完全随机分为3个日粮处理组,每组4个重复,每个重复12只鸡,进行42 d的饲养试验,旨在研究在肉仔鸡日粮中添加不同剂量的沙蒿籽粉对肉仔鸡免疫功能的影响。3种日粮分别是在基础日粮中添加0、0.1%和0.5%的沙蒿籽粉配合组成。结果表明,日粮中添加0.1%和0.5%沙蒿籽粉后,21和42日龄免疫器官指数无明显变化(P>0.05);21 d时s,CD4含量在组间差异不显著(P>0.05)4;2 ds,CD4在添加组含量均高于对照组,其中0.1%组显著升高(P<0.05)。此外,不论前期或后期,沙蒿籽粉添加组sCD8含量均极显著低于对照组(P<0.01);不论前期或后期,添加组IgA含量均低于对照组,差异极显著(P<0.01)。21日龄,添加组IgM含量低于对照组,差异显著(P<0.05);42日龄,添加组IgM含量与对照组相比呈下降趋势,差异不显著(P>0.05)。21和42日龄,添加组IgG含量均极显著高于对照组(P<0.01);此外,21和42日龄时,新城疫抗体滴度均没有明显变化。 相似文献
7.
研究拟通过甘露聚糖、海带多糖与益生素合用(简称甘海益)来替代黄芪多糖。动物试验结果表明,整个试验周期,甘海益组的料肉比低于黄芪多糖组,两组差异显著(P<0.05);42 d,甘海益组脾脏指数较高,与对照组和黄芪多糖组差异显著(P<0.05);35~42 d,甘海益组法氏囊指数高于黄芪多糖组(P<0.01或P<0.05);养殖前期,新城疫抗体效价各组差异不显著(P>0.05),35 d后,甘海益组新城疫抗体效价较高,与对照组和黄芪多糖组差异显著(P<0.05);黄芪多糖组和甘海益组对IgG的生成均有促进作用,42 d,与对照组差异显著(P<0.05),黄芪多糖和甘海益两组间差异不显著(P>0.05);35 d时,甘海益组血清总抗氧化能力（T-AOC）数值较高,与对照组和黄芪多糖组差异极显著(P<0.01);14 d,黄芪多糖组MDA含量较低,与对照组和甘海益组相比差异显著(P<0.05);21~28 d时,黄芪多糖和甘海益组MDA含量均较低,与对照组相比差异显著(P<0.05)和极显著(P<0.01)。甘露聚糖、海带多糖与益生素合用具有协同作用,可阶段性地提高机体的特异性免疫和非特异性免疫机能,提高肉鸡机体抗氧化能力,降低机体组织受损伤程度,可较好地替代黄芪多糖。 相似文献
8.
本试验旨在研究不同免疫应激对肉仔鸡生长性能、养分消化、血清生化指标和肉品质的影响.选取540只1日龄Cobb500肉仔鸡,随机分为5组(对照组、常免组、简免组、免疫亢进组和免疫抑制组),每组设9个重复,每个重复12只鸡.常免组和简免组按试验要求进行疫苗免疫,亢进组和抑制组在常免基础上分别注射细菌脂多糖(LPS)和环磷酰胺(CPM).21日龄和42日龄屠宰取样,检测相关指标.结果表明:1)不同免疫应激对肉仔鸡料重比无显著差异(P>0.05);无免组和简免组肉仔鸡采食量和体增重与常免组无显著差异(P>0.05),LPS亢进和CPM抑制均极显著降低肉仔鸡全期体增重(P<0.01),且LPS亢进显著降低肉仔鸡全期采食量(P<0.05);2)不同免疫应激对能量表观消化率无显著差异(P>0.05);除简免组干物质表观消化率与无免组差异不显著(P>0.05)外,不同免疫应激均极显著或显著降低了肉仔鸡中后期干物质表观消化率(P<0.01)和粗脂肪表观消化率(P<0.05);肉仔鸡中后期免疫抑制组粗蛋白质回肠表观消化率显著降低(P<0.05);3)CPM抑制极显著增大了肌肉滴水损失(P<0.01).结果提示:合理控制饲养环境条件下,无免疫不影响肉仔鸡生长性能,而免疫应激降低肉仔鸡对饲粮干物质、回肠粗蛋白质、粗脂肪的表观消化率,影响采食量和体增重,不利于肉仔鸡生长和生产. 相似文献
9.
研究灌胃猪血浆IgG提取物对小白鼠免疫功能的影响。采用腹腔注射环磷酰胺制造试验小白鼠的免疫功能抑制模型,选用齐墩果酸为阳性对照组,猪血浆IgG提取物为试验组,预饲5d后,然后将猪血浆IgG提取物按每千克体重45、90和180mg3种剂量给小白鼠连续灌胃10d后测定小白鼠免疫水平的变化。小白鼠灌胃猪血浆IgG提取物后,对抗由环磷酰胺引起的小白鼠免疫器官萎缩有一定的保护作用,并对环磷酰胺所致动物DTH反应低下有一定的对抗作用;能显著增加小白鼠血清中IgG的含量和免疫球蛋白的含量(P<0.05),能显著增加小白鼠血清中球蛋白的含量(P<0.05),灌胃猪血浆IgG提取物能显著提高小白鼠的免疫功能。 相似文献
10.
牛膝多糖对雏鸡禽流感免疫效果的影响 总被引：2，自引：0，他引：2
研究牛膝多糖在对雏鸡进行禽流感灭活苗免疫中的影响。选用30只肉仔鸡分3组,每组10只鸡。所有肉仔鸡于2和6周龄分别进行禽流感灭活苗的首免和二免。免疫的同时,试验1组每羽鸡肌肉注射0.5 mL 8 mg/mL牛膝多糖;试验2组每羽鸡肌肉注射0.5 mL 4mg/mL牛膝多糖;对照组全价料正常饲喂,只免疫不注射多糖。测定脾、胸腺和法氏囊指数;分别在首免后14和21 d及二免后14和21 d测定禽流感血凝抑制抗体的滴度。与对照组相比,试验1组和试验2组雏鸡的脾、胸腺和法氏囊指数,均显著提高(P0.05);二免后禽流感血凝抑制抗体的滴度显著升高(P0.05),且牛膝多糖高剂量组显著高于低剂量组(P0.05)。在一定范围内高剂量的牛膝多糖能提高雏鸡的免疫器官指数和禽流感血凝抑制抗体的滴度,对雏鸡禽流感免疫有一定促进作用。 相似文献
11.
Akhtar M Hai A Awais MM Iqbal Z Muhammad F ul Haq A Anwar MI 《Veterinary parasitology》2012,186(3-4):170-177
This paper reports the immunostimulatory and protective effects of Aloe vera extracts (aqueous and ethanolic) against coccidiosis in industrial broiler chickens. The study was divided into two experiments. Experiment-I was conducted for the evaluation of immunostimulatory activity of A. vera and experiment-II demonstrated the protective efficacy of A. vera extracts against coccidiosis in chickens. Results of the experiment-I revealed significantly higher (p<0.05) lymphoproliferative responses in chickens administered with ethanolic extract of A. vera as compared to those administered with aqueous extract and control group. Microplate haemagglutination assay for humoral response on day 7th and 14th post primary and secondary injections of sheep red blood cells (SRBCs) revealed significantly higher (p<0.05) anti SRBC antibody (total Igs, IgG and IgM) titers in chickens of experimental groups as compared to the control group. None of the extracts, however, demonstrated significant effects on the development of lymphoid organs. Results of experiment-II revealed maximum protection (60%) in chickens administered with aqueous Aloe extract as compared to the ethanolic extract administered chickens (45%). Mean oocysts per gram of droppings in the control group was significantly higher (p<0.05) as compared to the chickens in both the experimental groups. Chickens administered with aqueous Aloe extract showed a minimal mean lesion score (2.3) followed by those administered with ethanolic Aloe extract (2.6) and control chickens (3.05) for caeca, and a similar pattern was observed for intestinal lesion scoring. Further, significantly higher weight gains and antibody titers (p<0.05) were observed in chickens administered with A. vera extracts as compared to those in the control group. It was concluded that A. vera may be a potential and valuable candidate to stimulate the immune responses and can be used successfully as an immunotherapeutic agent against coccidiosis in industrial broiler chickens. 相似文献
12.
Reduced serologic response to Newcastle disease virus in broiler chickens exposed to a Chinese field strain of subgroup J avian leukosis virus 总被引：3，自引：0，他引：3
In this study, a Chinese field strain of subgroup J avian leukosis virus (ALV-J), NX0101, was studied for its immunosuppressive effects in both commercial broilers and SPF white Leghorn chickens infected at 1 day of age. Our data demonstrated that NX0101 induced much more significant body and immune organ weight loss in the infected commercial broiler chickens in an earlier age than that in the SPF white Leghorn chickens. At the same time antibody responses to vaccinations of Newcastle disease virus (NDV) and infectious bursa disease virus (IBDV) in the NX0101-infected chickens were also evaluated and compared between the commercial broiler chickens and the SPF white Leghorn chickens. Compared with the control group of chickens, the hemagglutination inhibition (HI) antibody response to NDV vaccines was significantly reduced in the NX0101-infected commercial broiler chickens from as early as 20 days after vaccination. However, no significant difference in HI antibody response was seen when HI titers reached their peaks in the NX0101-inoculated and control SPF white Leghorn chickens, except it declined significantly faster in infected birds. Neither of these two types of chickens showed significant decrease of antibody response to IBDV vaccination. Herein, we conclude that this NX0101 strain of ALV-J could selectively suppress humoral immune reactions to NDV, especially in broilers. But challenge experiments were not conducted and, therefore, it cannot be known if decreased antibody levels correlated with decreased protection against NDV in this case. 相似文献
13.
本试验旨在研究霉变饲料和／或添加复方霉菌毒素吸附剂对肉鸡人工感染发生的影响。选取140羽1日龄健康的AA肉鸡随机分成7个组．每组4个重复。第1组为空白对照组,饲喂基础日粮;第2组饲喂霉变饲料;第3～7组以混合感染病鸡病变组织匀浆饮水制作人工感染疾病模型;第3～7组分别饲喂基础日粮、霉变饲料、霉变饲料＋复方霉菌毒素吸附剂、霉变饲料＋复方霉菌毒素吸附剂＋抗氧化剂、霉变饲料＋复方霉菌毒素吸附剂＋抗氧化剂＋中药增免剂。试验期35d。结果发现,肉鸡摄入霉变饲料与摄入正常饲料相比,提高肉鸡料重比和死淘率,显著降低肉鸡增重、胸腺指数、法氏囊指数,显著降低血清IBDV、H9N1v和NDV抗体水平（P〈0．05）。给人工感染肉鸡饲喂霉变饲料与饲喂正常饲料相比．显著提高料重比和死淘率,显著降低肉鸡增重、胸腺指数和脾脏指数,显著降低血清IFN-1、IL-2、IL-4和IL-12含量以及LTR,显著降低血清IBDV、H9N1V和NDV抗体水平（P〈0．05）。霉变饲料中添加复方霉菌毒素吸附剂能显著提高混合感染肉鸡增重,并降低料重比和死淘率（P〈0．05）,显著提高胸腺指数、法氏囊指数（P〈0．05）,显著提高血清IBDV、H9N1V和NDV抗体水平（P〈0．05）。可见,霉变饲料可导致肉鸡免疫功能抑制,加重肉鸡混合感染病情;复方霉菌毒素吸附剂能有效缓解霉变饲料对人工感染肉鸡生产性能、免疫功能和病情的不利影响,抗氧化剂和免疫功能增强剂可减轻霉变饲料的毒性作用。 相似文献
14.
通过观察疫苗免疫后鸡群的生长情况和临床症状,检测免疫后不同时期鸡群的ELISA抗体水平和攻毒保护率以及对鸡新城疫（ND）疫苗免疫后不同时间鸡群的HI抗体水平,评价和检测鸡传染性法氏囊病活疫苗（B87株 CA株 CF株）在石家庄地区临床应用的安全性、免疫效果、免疫持续期以及是否会产生免疫抑制。结果表明,试验疫苗接种蛋鸡和肉鸡后,均没有观察到因疫苗引起的不良反应,对蛋鸡和肉鸡均安全。疫苗接种蛋鸡后14 d、28 d、42 d、60 d和90 d,接种肉鸡后14 d、21 d、36 d攻毒保护率均达80%以上;商品蛋鸡接种疫苗后90 d其ELISA抗体水平仍高达6500以上。ND HI抗体检测结果表明,试验疫苗接种后对ND疫苗的免疫应答无显著影响,不引起免疫抑制。结论：试验疫苗安全、有效,质量稳定,适用于预防鸡传染性法氏囊病。 相似文献
15.
使用鸡新城疫-禽流感（H9N2 HP株）二联灭活疫苗分别免疫3、7、14日龄三组商品肉鸡各40羽,同时设一组空白对照组。各免疫组及对照组于3、7、14、21、28、35、42日龄采血检测新城疫、禽流感抗体,于21、28、35日龄进行禽流感病毒攻毒,对比不同日龄免疫组的抗体消涨情况及不同日龄禽流感攻毒结果。发现对照组随鸡日龄增加,新城疫与禽流感抗体逐渐下降,在42日龄时下降至0,而不同免疫组新城疫与禽流感抗体均先下降,21日龄左右开始上升,至35日龄新城疫与禽流感抗体升至6log2以上。3日龄免疫组的禽流感免疫保护效果最好,21、28、35日龄时禽流感强毒攻毒保护率均达100%;7日龄免疫组在21、35日龄时禽流感强毒攻毒保护率均达100%,28日龄时禽流感强毒攻毒保护率达70%;14日龄免疫组在28、35日龄时禽流感强毒攻毒保护率均达100%,21日龄时禽流感强毒攻毒保护率只达30%。试验表明,商品肉鸡选择3日龄免疫鸡新城疫-禽流感（H9N2 HP株）二联灭活疫苗时禽流感免疫保护效果最好,采用3日龄免疫程序可以提高新城疫与禽流感的免疫保护效果,减少养殖业的经济损失。 相似文献
16.
《畜牧与生物技术杂志(英文版)》2015,(3)
Background:As a member of the Paramyxoviridae group,Newcastle disease virus(NDV) is the key causative agent of Newcastle disease(ND) that attacks chickens,turkeys and other avian birds.Surviving birds showed lower feed utilization,growth performance or egg production,which results in severe economic losses.The purpose of this study was to determine the effect of different doses of NDV immunization on growth performance,plasma variables and immune response of broiler chickens.Methods:A total of 480 one-day-old Arbor Acres broilers were randomly administrated with 0,4,6 or 8 doses of NDV at 12 d and 28 d,respectively.Each group consisted often replicates with 12 birds each.Growth performance and organ weight were recorded.Plasma concentration of glucose,total protein,cholesterol,triglycerides and nonesterified fatty acid was determined using commercial kits.The concentration of plasma corticosterone and insulin was measured using commercially available radio immune assay kits.Serum antibody titer and peripheral blood lymphocyte proliferation were also recorded.Results:The results showed that NDV decreased body weight gain(BWG),and increased Feed:Gain ratio at 1-21 d at all doses(P 0.05).Plasma insulin concentration was lower in all immunization groups after the first immunization at12 d(P 0.01).The rest of the plasma indexes were not affected by NDV immunization,including glucose,total protein,cholesterol,triglycerides,nonesterified fatty acid,heterophil/lymphocyte ratio,as well as the proliferation of peripheral blood lymphocyte(P 0.05).Compared with the control group,NDV treatment elevated NDV antibody titer at 10 d after the first inoculation(P 0.05),and at d 5,9 and 13 after the second inoculation(P 0.05).Repeated NDV inoculation had no deleterious impacts on body composition at 42 d,and nutrient accretion rates at 8-42 d(P0.05).Conclusions:In conclusion,NDV challenge decreased BWG and feed efficiency in earlier stage of growth.However,NDV treatment at 6 doses down-regulated the Feed:Gain ratio by 6.36%throughout the whole growing period.These data suggest that appropriate lower doses of NDV inoculation increase feed efficiency of broiler chickens. 相似文献
17.
ZHANG Zhen-hua LI Lin ZHANG Jian-wei SHEN Jia SHI Ai-hua HUANG Feng-jun ZHENG Xiao-lan JIANG Bei-yu 《中国畜牧兽医》2016,43(5):1374-1378
1-day-old SPF chickens and commercial Jingfen chickens were vaccinated with IBD immune complex(IC) vaccine, NDV La Sota vaccine were inoculated simultaneously every one week and every two weeks.NDV La Sota immunization alone was as the control group.At the 2nd, 3rd, 4th and 5th week post inoculation, blood samples were taken and the ND HI antibody were tested.Experimental chickens were challenged with high virulent NDV at the 5th week post inoculation, the protective rate of each group was calculated.The results showed the ND HI antibody were not significant different in the combined immunization of IBD IC vaccine priming and NDV La Sota vaccine boost and NDV La Sota vaccine alone immunization (P>0.05).The results indicated that IBD IC vaccine has no immunosuppression on NDV La Sota vaccine in SPF chickens and commercial Jingfen chickens. 相似文献
18.
重组禽流感病毒灭活疫苗(H5N1亚型,Re-1株)免疫鸡抗体消长规律及免疫程序的初步研究 总被引：4，自引：0，他引：4
研究新型重组H5N1亚型禽流感灭活疫苗对种鸡和肉鸡的免疫原性,并对雏鸡母源抗体和免疫后的抗体进行动态观察,根据试验结果推荐该疫苗对鸡的免疫程序。用HI方法检测种鸡、肉鸡的母源抗体和免疫抗体,根据母源抗体的衰减和免疫抗体的消长规律确定首免和再免日龄。结果表明种鸡的母源抗体约能维持10 d多;0.3 mL/羽首免后10 d HI抗体就可达到6.40 log2,3-5周达到高峰期,至免疫17周后(19周龄)HI抗体水平仍然维持在4.88 log2;19周龄时0.5 mL/羽进行二免,有效抗体能维持约20周;280日龄0.5 mL/羽三免后抗体水平均一,下降缓慢,至种鸡淘汰时(三免后29周)抗体水平仍能维持在5.32 log2。肉鸡母源抗体约能维持7d,10日龄时0.3 mL/羽免疫,有效抗体能维持到上市。新型重组H5N1亚型禽流感灭活疫苗对鸡的免疫原性确实。 相似文献
19.
鸽禽Ⅰ型副粘病毒油佐剂灭活苗对雏鸡免疫效果评价 总被引：1，自引：1，他引：0
用鸽Ａ／ＰＭＶ－１油佐剂灭活苗与ＮＤＶ油佐剂灭活苗分别免疫雏鸡,免疫后２１ｄ抗体水平达到峰值,免疫后４２ｄ用新城疫强毒对两种疫苗免疫鸡分别进行攻击,鸽Ａ／ＰＭＶ－１油佐剂灭活苗免疫组保护率为７３．３３％,ＮＤＶ油佐剂灭活苗免疫组保护率为９９．６７％。 相似文献
20.
为了研究苜蓿多糖对新城疫弱毒苗免疫鸡所产生的免疫调节作用,本研究选择1日龄海兰雏鸡120羽,随机分为4组,每组30羽,7日龄每羽用鸡新城疫活疫苗（La Sota株）免疫,28日龄以相同剂量进行加强免疫。其中3个试验组从7日龄开始分别肌肉注射低、中、高剂量（10、25、50μg/mL）的苜蓿多糖,对照组注射生理盐水,1次/d,连用7 d。于14、21、28、35、42、49、56日龄,通过静脉采血,用MTT法检测外周血淋巴细胞吸光度值（OD值）;同时用微量法检测血清中新城疫病毒血凝抑制（HI）抗体效价。结果显示,苜蓿多糖能够显著提高外周血淋巴细胞OD值,中剂量组（25μg/mL）淋巴细胞OD值最大;苜蓿多糖能够显著提高血清中新城疫病毒HI抗体效价,中剂量组免疫后期抗体存在时间长,抗体高峰期持续时间也长。本试验表明合适剂量苜蓿多糖对新城疫弱毒苗的免疫效果有明显的增强作用。 相似文献