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1.
A questionnaire concerning the diagnosis and treatment of bovine mastitis was sent to all 350 Swedish food animal practitioners. 287 (82%) of the questionnaires were returned. One of the main aims was to establish if Swedish food animal practitioners used a common therapeutic regime that could be used as a control treatment in future clinical trials.It was found that many factors of importance for the clinical diagnosis of mastitis such as body temperature, duration of the symptoms etc. often were not considered. On the other hand 60–70% of the practitioners regularly took milk samples to obtain a bacteriological diagnosis. Approximately 40% of the veterinarians cultured the milk samples in their home laboratory.Basic measures like frequent emptying of the udder were recommended by only 40–50% of the veterinarians.All responding field veterinarians used the systemic route for administering antibiotics when treating cases of acute, clinical bovine mastitis. The drug of choice, initially, in these cases was benzylpenicillinprocain, which was used by 65–75% of the veterinarians. Twentyfive percent used a broadspectrum antibiotic, most commonly a combination of penicillin and streptomycin. A minority (5%) directed their initial therapy towards gramnegative bacteria.About 30–40% supported the systemic therapy with intra-mammaries. Other drugs such as NSAID, corticosteroids and oxytocin was used, on a regular basis, by only about 10% of the practitioners. 相似文献
2.
This report was delineated to study the clinical, bacteriological and therapeutic aspects concerned with acute coliform mastitis
in buffaloes. Bacteriological examination of 80 quarter milk samples obtained aseptically from 56 buffaloes with acute mastitis
revealed that coliform bacteria was the most common pathogen (45 cases) followed by Staphylococcus aureus (seven cases) then Streptococcus uberis (three cases), and Streptococcus agalactiae (one case). Clinically, hotness, swelling and painful reaction with serous excretion containing clots was recorded in buffaloes
with coliform mastitis. The efficacy of ceftiofur was evaluated in the treatment of buffaloes with acute coliform mastitis.
Parenteral ceftiofur neither improved clinical signs nor returned milk to pre-infection production level, whereas intramammary
ceftiofur and combination of intramammary with parenteral ceftiofur improved the clinical signs in 10/15 and 12/15 buffaloes,
respectively. On quarter level, 3/17, 12/17 and 15/21 quarters recovered in groups received parenteral, intramammary and combination
therapy, respectively. This study demonstrates that systemic ceftofur is not effective in the treatment of clinical coliform
mastitis in buffaloes. 相似文献
3.
Farmers recorded the clinical signs of cows with clinical mastitis and submitted milk samples for bacteriological examination, so that the clinical signs could be correlated with the bacteriological findings. Odds ratios for the demeanour of the cow, the appearance of the milk, milk yield, udder texture, and the administration of parenteral antibiotics were calculated for mastitis cases classified in terms of their microbiology as either enterobacteriaceae, major Gram-positive pathogens, minor pathogens, 'no growths' or 'all other pathogens'. Animals infected with enterobacteriaceae had the highest odds of being reported as having a reduced milk yield, swollen or hard udders, watery milk and/or being systemically sick. A logistic regression model was used to predict the Gram-staining characteristics of the bacteria causing clinical mastitis. The clinical findings found to be significant predictors in the model were the demeanour of the cow and its milk yield. The regression model was used as a basis for a predictive test. Using a test data set, the sensitivity of the test was 28 per cent, its specificity was 96 per cent, the positive predictive value was 74 per cent and the negative predictive value was 80 per cent. The overall accuracy of these predictions was 79 per cent. 相似文献
4.
Waage, S., P. Jonsson and A. Franklin: Evaluation of a cow-side test for detection of Gram-negative bacteria in milk from cows with mastitis. Acta vet. scand. 1994, 207-211.– A modified Limulus amebocyte lysate (LAL) cow-side test was evaluated under field conditions. The principle of the test is to visualize reactions between test components and endotoxin from the cell wall of Gram-negative bacteria. The practical purpose is to detect such bacteria in mastitic milk. Secretions from 789 udder quarters with clinical mastitis were examined by the LAL-test. Parallel quarter milk samples were sent to a mastitis laboratory for microbiological examination. Eleven veterinary surgeons in three veterinary districts in Norway performed the field investigations. Results of the LAL-test and culture agreed in 93% of all milk samples tested, agreement measured by kappa being 0.63. The sensitivity of the test in detecting Gram-negative bacteria was 63%, while the specificity was 97%. The predictive value of a positive test result was 70%, the figure being somewhat higher (75%) when the material was limited to milk samples without antibiotic residues. The predictive value of a negative test result was 95%. The LAL-test is considered to constitute a valuable cow-side test for the veterinary practitioner, aiding the selection of antibacterial drug of choice for the initial treatment of clinical mastitis. 相似文献
5.
White ME Glickman LT Barnes-Pallesen FD Stem ES Dinsmore P Powers MS Powers P Smith MC Jasko D 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(5):218-220
We examined the ability of clinicians to predict the causative organism of bovine mastitis in our practice. We obtained 118 milk culture results from 112 mastitic cows and compared the culture results to the predictions of clinicians at the time of milk sample collection. Sixty of 118 culture results were accurately predicted. The positive predictive value for coliform mastitis was 42% and the negative predictive value was 79% in a study population with a 31% prevalence of coliform mastitis. The proportion of coliform positive cultures accurately predicted was significantly greater than that expected by chance (p < 0.02). 相似文献
6.
M E White L T Glickman F D Barnes-Pallesen E S Stem P Dinsmore M S Powers P Powers M C Smith M E Montgomery D Jasko 《The Cornell veterinarian》1986,76(4):342-347
We tested an equation, which had been developed previously using discriminant analysis, for predicting whether a cow has coliform mastitis. Variables indicating a high probability of coliform infection included history of previous mastitis in the affected quarter, weakness, clear or white color of milk, water consistency of the milk, swelling of the udder, lack of previous mastitis in other quarters, lack of palpable udder abscesses, and a high body temperature. Application of this predictive equation to 114 cows with mastitis to determine if they would have coliform organisms cultured from the affected quarters resulted in an accuracy of 71% (sensitivity = 0.42, specificity = 0.85), compared to an accuracy of 62% (sensitivity = .64, specificity = .61) for cowside prediction by the attending clinicians. Changing the cutoff score of the discriminant rule so that the sensitivity of the discriminant prediction was similar to that of the clinicians yielded an accuracy of 64% (sensitivity = .64, specificity = .64). 相似文献
7.
Partial budget analysis of vaccinating dairy cattle against coliform mastitis with an Escherichia coli J5 vaccine 总被引：3，自引：0，他引：3
Partial budget analysis of clinical coliform mastitis prevention supported vaccination of dairy cows with an Escherichia coli J5 vaccine. Increased profits of $57/cow lactation were predicted using a computer spreadsheet derived partial budget with generalized herd input data. Herd vaccination programs were predicted to be profitable when greater than 1% of cow lactations resulted in clinical coliform mastitis. Herd vaccination programs were predicted to be profitable at all herd milk production levels. 相似文献
8.
9.
真菌性乳房炎病原菌的分离鉴定与流行病学调查 总被引：7，自引：0，他引：7
本研究对山东省济南、泰安、临沂三地区共386份乳房炎奶样进行了真菌的分离鉴定与流行情况调查。经鉴定其病原为白色念珠菌。调查结果表明真菌性乳房炎在不同地区均有发生。个体养殖户奶牛奶样的真菌阳性率（19．3％）明显高于规模化养殖场（7．62％）；临床型乳房炎的真菌阳性率（13．11％）高于隐型乳房炎（3．75％）。本文的调查结果为更好地了解真菌在我国奶牛业的感染情况和采取有效的防治措施提供了一定的依据。 相似文献
10.
为评价复方阿莫西林乳房注入剂对泌乳期奶牛临床型乳房炎的治疗效果,在甘肃某两个牛场选择70头自然发生的临床型乳房炎奶牛进行临床试验。将患病奶牛随机分为试验组(n=36头)和对照组(n=34头)。试验用药和对照用药分别为郑州百瑞动物药业有限公司和齐鲁动物保健品有限公司提供的复方阿莫西林乳房注入剂。每个感染乳区注入3 g药物,每12 h注射1次,连续用药3 d。在用药前、停药后7 d及14 d采集各组奶牛的奶样进行细菌学检测及乳汁体细胞计数。通过临床观察、细菌清除率及体细胞计数变化对临床疗效进行评价。停药后14 d,试验组和对照组的临床治愈率分别为58.3%和61.8%,细菌总清除率分别为94.12%和93.55%。两组奶样中的SCC在治疗后均显著下降(P<0.01)。两组临床疗效无统计学差异(P>0.05),表明试验用药对奶牛临床型乳房炎具有良好的治疗效果。 相似文献
11.
D J Wilson P C Bartlett J H Kirk R W Mellenberger E C Mather 《American journal of veterinary research》1991,52(7):1110-1116
Milk samples were collected at onset of 508 episodes of clinical mastitis on a 1,700-cow dairy farm in Michigan. Daily milk production and disease events were recorded for all cows in the herd. Despite statistical association with severity of mastitis, this association was too weak for N-acetyl-beta-D-glucosaminidase (NAGase) activity to be of great value as a prognostic test for clinical mastitis. High milk NAGase activity was significantly (P less than 0.0001) associated with: increased duration of treatment; increased duration of clinical signs of mastitis; decreased daily milk production; and increased risk of the cow being culled because of mastitis. The NAGase value was combined with days in milk production, baseline milk production before mastitis onset, parity, and season of onset to predict the outcome of clinical cases as measured by the first 3 aforementioned variables. Statistical models explained little of the variability among cows in duration of treatment (R2 = 0.11), duration of clinical signs of infection (R2 = 0.11), and milk production change (R2 = 0.09). 相似文献
12.
W D Hueston K H Hoblet G Y Miller M L Eastridge 《Journal of the American Veterinary Medical Association》1990,196(12):1956-1961
A telephone survey was conducted of 50 randomly selected Ohio-licensed veterinarians engaged in dairy practice. The survey's purpose was to determine the extent of mastitis control services offered by practitioners and to assess their utilization of milk somatic cell count (SCC) data on individual cows available from the Dairy Herd Improvement Association (DHIA). During the preceding year, 96% (48/50) of practitioners surveyed had performed bacteriologic culture of milk samples. Practitioners were twice as likely to have performed culture on milk from mastitic cows that failed to respond to treatment as they were to have performed culture for purposes of identifying pathogen trends within a herd. Veterinarians in predominantly dairy practices were more likely to have completed bacteriologic examination of milk in their own laboratories than were veterinarians who were engaged in less than 50% dairy practice (P = 0.016). Most veterinarians (83%) reported that coagulase test results were available or that Staphylococcus aureus was differentiated from other staphylococcal species. Streptococcus agalactiae was not differentiated from other streptococcal species by 35% of practitioners surveyed. For veterinarians with clients enrolled in the DHIA, 91% (43/47) reported looking at, discussing, or otherwise using the DHIA records. Eighty-one percent (35/43) of veterinarians who had clients using services from the DHIA reported that clients also received individual cow milk SCC results. Veterinarians engaged in predominantly, dairy practice expressed a greater familiarity with the linear score method of SCC reporting than did veterinarians whose practices were less than 50% dairy (P = 0.085); however, both groups reported a preference for raw SCC data.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
13.
[目的]为了摸清辽西地区规模化奶牛场奶牛隐性乳房炎发病情况,[方法]本研究应用美国加州奶牛乳房炎检测方法(California mastitis test, CMT)对规模化奶牛场奶牛定期采集奶样进行隐性乳房炎动态监测。通过临床检查和CMT方法对辽西地区5个规模化奶牛养殖场共1 326头泌乳期奶牛进行了隐性乳房炎的监测,[结果]统计结果显示,隐性乳房炎发病率高达41.25%,发病率以夏秋季节最高,随着年龄增大,胎次增多,泌乳增加,乳房炎的发病率增高。[结论]研究结果表明辽西地区奶牛乳房炎发病情况较为严重,需要规模化奶牛养殖场高度重视,同时为该地区奶牛场乳房炎的防控提供了理论依据。 相似文献
14.
Clinical mastitis in dairy cattle in Ontario: frequency of occurrence and bacteriological isolates. 总被引：5，自引：0，他引：5 
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下载免费PDF全文 J M Sargeant H M Scott K E Leslie M J Ireland A Bashiri 《The Canadian veterinary journal. La revue veterinaire canadienne》1998,39(1):33-38
The objective of this study was to describe the frequency of occurrence of clinical mastitis in dairy herds in Ontario. The study group consisted of 65 dairy farms involved in a 2-year observational study, which included recording all clinical mastitis cases and milk sampling of quarters with clinical mastitis. Lactational incidence risks of 9.8% for abnormal milk only, 8.2% for abnormal milk with a hard or swollen udder, and 4.4% for abnormal milk plus systemic signs of illness related to mastitis were calculated for 2840 cows and heifers. Overall, 19.8% of cows experienced one or more cases of clinical mastitis during location. Teat injuries occurred in 2.1% of lactations. Standard bacteriology was performed on pretreatment milk samples from 834 cows with clinical mastitis. The bacteria isolated were Staphylococcus aureus (6.7%), Streptococcus agalactiae (0.7%), other Streptococcus spp. (14.1%), coliforms (17.2%), gram-positive bacilli (5.5%), Corynebacterium bovis (1.7%), and other Staphylococcus spp. (28.7%). There was no growth in 17.7% of samples, and 8.3% of samples were contaminated. Clinical mastitis is a common disease in dairy cows in Ontario; approximately 1 in 5 cow lactations have at lease one episode of clinical mastitis. There is, however, considerable variation in the incidence of clinical mastitis among farms. The majority of 1st cases of clinical mastitis occur early in lactation, and the risk of clinical mastitis increases with increasing parity. Environmental, contagious, and minor pathogens were all associated with cases of clinical mastitis. 相似文献
15.
Indicators of inflammation in the diagnosis of mastitis 总被引：18，自引：0，他引：18
Pyörälä S 《Veterinary research》2003,34(5):565-578
Mastitis affects the quality of milk and is a potential health risk for the other cows. In a well managed dairy herd, in addition to clinical mastitis, subclinical mastitis should be efficiently detected. Bacteriological sampling is not feasible as a routine test to identify subclinical mastitis, and indirect tests of mastitis are more suitable for selecting cows with intramammary infections for subsequent bacteriological sampling. Mastitis affects the composition of milk, and the degree of changes depends on the infecting agent and the inflammatory response. Indicators of inflammation in the milk which can be determined using rapid, reliable and easy routine techniques, can be used for the early detection of mastitis. The measuring of the somatic cell count in milk is the standard method, but the analysis technique is problematic for routine use in herds. The most promising parameters for monitoring subclinical mastitis are milk N-acetyl-beta-D-glucosaminidase activity, lactose, and electrical conductivity along with some other indicators such as optical and milk flow measurements, preferably with an inter-quarter evaluation included in the test. Acute phase proteins, haptoglobin and serum amyloid A, are also potential candidates for mastitis monitoring. New mastitis detection systems which can be adapted into on-line use are urgently needed, since dairy units are growing bigger and automatic milking systems are being taken into use. 相似文献
16.
Quarter milk samples (n = 543) from 152 traditionally managed lactating camels (Camelus dromedarius) in Afar Region, north-eastern Ethiopia were examined to determine the prevalence of camel mastitis and identify its bacterial causes. Out of 152 camels examined, 19 (12.5%) were diagnosed as clinical mastitis cases based on clinical signs and bacteriological examinations. Of the 257 California Mastitis Test (CMT) positive quarter milk samples 162 (63.0%) yielded pathogenic bacteria. A positive correlation was observed between CMT positive results and presence of major pathogens in camel milk samples. The main mastitis pathogens isolated were Staphylococcus aureus, coagulase-negative staphylococci, Streptococcus agalactiae, S. dysgalactiae, and other species of streptococci, Pasteurella haemolytica and E. coli. Results of the present study suggest that mastitis in Afar camels is prevalent, Gram-positive cocci are the major isolates from camel milk samples and the CMT can be used as a screening test for the detection of mastitis in camels. 相似文献
17.
According to the present study the limulus amebocyte lysate test (LAL) seems to be a convenient test to detect endotoxin in milk from udder quarters with and without inflammation. The correlation between endotoxin concentration and the results from the bacteriological investigation of 79 milk samples was good (Table I). Determination of endotoxin in 20 milk samples from cases of acute clinical mastitis with high cell count and a negative bacteriological culture showed that all but one had an endotoxin concentration of greater than 1.0 ng/ml milk (Table II). By using a micromethod of the LAL it is possible to detect cases of mastitis caused by gram-negative bacteria about one hour after the sample has reached the laboratory. In a preliminary field study milk from 13 cases of acute clinical mastitis were tested by a modified limulus amebocyte lysate (LAL) test ("cowshed test"). A 100% correlation to bacteriological findings was observed (Table IV). By using the LAL test to detect mastitis cases caused by gram-negative bacteria great economic advantages and less risk for resistance problems can be achieved by using proper antibiotics. This is the fact in Sweden where the frequency of acute clinical mastitis caused by streptococci (100% of strains sensitive for penicillin) and Staphylococcus aureus (about 90% of strains sensitive for penicillin) is high (70-80%) and about 20% are caused by gram-negative bacteria, mostly E. coli. 相似文献
18.
Smallholder dairy farmers in Tanzania appear to be unaware of the subclinical mastitis situation in their cows. A cross-sectional study was carried out between June and September 2002 on smallholder dairy herds in the Dar es Salaam region. The study objectives were to establish the prevalence of subclinical mastitis and related risk indicators, and to assess their contribution to the occurrence of subclinical mastitis. Three field procedures based on the principles of herd health and production management were followed: clinical, farm and data inspection. The California mastitis test (CMT) was carried out on quarter milk samples to determine the prevalence of subclinical mastitis. A total of 182 lactating cows from 62 herds were investigated. Clinical inspection indicated that 3.8% of the lactating cows had clinical mastitis. Subclinical mastitis was detected in 90.3% of lactating cows screened. Farm inspection revealed that water scarcity, barn size, residual suckling, single udder-towel and dairy labourers as the most substantial (p < 0.05) risk indicators. Although most of the risk indicators studied were not found to be statistically significantly associated with the occurrence of subclinical mastitis, possibly owing to sample size and the presence of confounders, the epidemiological need to address such risk indicators cannot be overemphasized. 相似文献
19.
Investigation of mastitis problems on farms. 总被引：7，自引：0，他引：7
The production of high-quality milk is a high-priority issue for dairy farmers and milk processors. The investigation of mastitis problems on dairy farms is an area of increasing demand and is an ideal way for veterinary practitioners to increase involvement in production medicine programs. Goals for the amount of clinical and subclinical mastitis should be predefined on dairy farms, and a herd investigation initiated when needed. The use of a structured approach to mastitis problems can identify risk factors for infection, result in rapid resolution of mastitis problems, and hasten the application of appropriate preventive practices. 相似文献
20.
M E White L T Glickman M E Montgomery F D Barnes-Pallesen E S Stem P Dinsmore M S Powers P Powers M C Smith D Jasko 《The Cornell veterinarian》1987,77(1):13-20
Logistic regression was used to analyze the clinical findings (attributes) which predicted coliform mastitis in 113 dairy cattle, 36 of which had coliforms cultured from milk. Weakness of the cow, swelling of the udder, decreased body temperature and watery consistency of the milk were selected for the model. An analysis was then done to find the attributes which clinicians used when predicting that a cow would have a coliform cultured. Clinicians appeared to use water consistency of the milk, shivering, firmness of the udder, pulse rate, elevated body temperature, and respiratory rate. In a final analysis the clinicians' predictions were forced into the model to determine which attributes might be used by clinicians to increase diagnostic accuracy. Inclusion of weakness of the cow, swelling of the udder, decreased temperature of the cow, and duration of mastitis of less than 24 hours increased accuracy over clinical prediction alone. Accuracy of cowside diagnosis might be increased if more attention were paid to these attributes when making a diagnosis of coliform mastitis. 相似文献