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1.
Cuixia Sun Guangming Zhang Meng Li Xiaopeng Wang Guodong Zhang Yanchen Tian Zeli Wang 《Euphytica》2010,174(2):219-229
Maize dwarf mosaic is one of the devastating and wide spread viral diseases in the world. The present investigation was carried
out to develop DNA markers closely linked to the resistance gene mdm1 (t). Linkage between the markers and phenotypes was confirmed by analyzing an F2 population obtained from a cross between a resistant parent ‘Huangzaosi’ and a susceptible parent ‘Mo17(478)’. Four AFLP
markers were found in the maize dwarf mosaic resistant plants. By using (BSA) bulked segregant analysis, two of the four AFLP
markers were transformed into Sequence-characterized amplified regions markers (SCARs), nominated Rsun-1 and Rsun-2. The two
amplified fragment length polymorphism (AFLP) markers, RHC-1and RHC-2, from the amplification products of primer combination
E-AGC/M-CAA and E-AGC/M-GAA, showed linkage with the mdm1 (t) gene in a genetic distance 1.6 and 2.0 cM, respectively. The results indicate that the new SCAR markers will be valuable
to distinguish resistant plants from susceptible plants in plantlets growing in seedbeds. The markers developed in this study
are suitable for marker-assisted selection for maize dwarf mosaic resistance. 相似文献
2.
Simple sequence repeat markers linked to a major QTL controlling pod shattering in soybean 总被引：3，自引：0，他引：3
H. Funatsuki M. Ishimoto H. Tsuji K. Kawaguchi M. Hajika K. Fujino 《Plant Breeding》2006,125(2):195-197
Shattering of soybean pods prior to harvest leads to a reduction in yield. In order to identify simple sequence repeat (SSR) markers linked to quantitative trait loci (QTLs) conditioning pod shattering, QTL analysis was conducted using an recombinant inbred line (RIL) population segregating for this trait. The degrees of pod‐shattering resistance were evaluated by heat treatment applied to pods harvested from plants in the field and in a growth chamber. Composite interval mapping identified one major QTL between SSR markers Sat_093 and Sat_366 on linkage group J for both environments. The position and the effect of this QTL were confirmed in an F2 population derived from a cross between the pod shattering‐susceptible parental cultivar and a pod shattering‐resistant RIL. The SSR markers linked to the major QTL will be useful for marker‐assisted selection in soybean‐breeding programmes. 相似文献
3.
Prakash R. Arelli Vergel C. Concibido Lawrence D. Young 《Journal of Crop Science and Biotechnology》2010,13(3):163-167
Worldwide, soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is the most destructive pathogen of soybean [Glycine max (L.) Merr.]. Crop losses are primarily mitigated by the use of resistant cultivars. Nematode populations are variable and
have adapted to reproduce on resistant cultivars over time because resistance primarily traces to two soybean accessions,
Plant Introduction (PI) 88788 and Peking. Soybean cultivar Hartwig, derived primarily from PI437654, was released for its
comprehensive resistance to most SCN populations. A synthetic nematode population (LY1) was recently selected for its reproduction
on Hartwig. The LY1 nematode population currently infects known sources of resistance except soybean PI567516C; however, the
resistance to LY1 has not been characterized. The objective of this study was to identify quantitative trait loci (QTLs) underlying
resistance to the LY1 SCN population in PI567516C, identify diagnostic DNA markers for the LY1 resistance, and confirm their
utility for marker-assisted selection (MAS). Resistant soybean line PI567516C was crossed to susceptible cultivar Hartwig
to generate 105 recombinant inbred lines (F2-derived F5 families). QTLs were mapped using simple sequence repeats (SSRs) covering 20 Linkage Groups (LGs) and three diagnostic markers,
Satt592, Satt331, and Sat_274, were identified on LG O. These markers have a combined efficacy of 90% in identifying resistant
lines in a second cross that has been generated by crossing a susceptible cultivar 5601T with resistant PI567516C. F2-derived F4 segregating population was used in MAS to identify resistant lines. 相似文献
4.
Akhouri Nishant Bhanu Mahendra Narain Singh Kartikeya Srivastava 《Plant Breeding》2019,138(2):202-206
Six intervarietal crosses involving two resistant and three susceptible genotypes of mungbean were attempted with the objectives to determine the mode of inheritance of gene‐specific Mungbean Yellow Mosaic Virus (MYMV) resistance. An infector row technique along with artificial inoculation was used for evaluating parents, F1, F2 and F3 plants for MYMV resistance. Disease scoring for MYMV indicated that F1s were highly susceptible as were the susceptible parents while resistant parent exhibited resistant reaction. The F2 progeny segregated in the ratio of 9 S:3 MS:3 MR:1 R suggesting that the resistance was governed by digenic recessive genes (rm1 and rm2). When one gene (rm1) was present in the homozygous recessive condition in different plants, it conferred moderately susceptible (MS) reaction, whereas when other gene (rm2) was in homozygous condition, moderately resistant (MR) reaction was obvious. When both genes (rm1 and rm2) were present together in the homozygous recessive condition, resistant reaction (R) was observed. The F2 segregation explained on the basis of phenotypic expression was further confirmed by F3 segregation. 相似文献
5.
Eveline Teixeira Caixeta Aluízio Borém Samir de Azevedo Fagundes Silvia Niestche Everaldo Gonçalves de Barros Maurílio Alves Moreira 《Euphytica》2003,134(3):297-303
The existence of genetic variability for angular leaf spot (ALS) resistance in the common bean germplasm allows the development
of breeding lines resistant to this disease. The BAT 332 line is an important resistance source to common bean ALS. In this
work we determined the inheritance pattern and identified RAPD markers linked to a resistance gene present in BAT 332. Populations
F1, F2,BCs and BCr derived from crosses between BAT 332 and cultivar Rudá were used. Rudá is a commercial cultivar with carioca
type grains and susceptible to ALS. The resistance of BAT 332 to race 61.41 of the pathogen was confirmed. Segregation analysis
of the plants indicated that a single dominant gene confers resistance. For identification of RAPD markers linked to the resistance
gene, bulk segregant analysis (BSA) was used. Two RAPD markers,OPAA07950 and OPAO12950, linked in coupling phase at 5.10 and 5.83 cM of this gene, respectively, were identified. These molecular markers are important
for common bean breeders and geneticists as source of genetic information and for marker assisted selection in breeding programs.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
6.
Genetic mapping for resistance to gray leaf spot in maize 总被引：1，自引：0，他引：1
Fernando Cesar Juliatti Michelle Gonçalves Pedrosa Heyder Diniz Silva Junia Vianna Corrêa da Silva 《Euphytica》2009,169(2):227-238
The molecular marker technology has been used on mapping of quantitative trait loci (QTLs) associated with plant resistance.
The objectives of this research were to estimate genetic parameters and to map genomic regions involved in the resistance
to gray leaf spot in maize. Ninety F3 families from the BS03 (susceptible) and BS04 (resistant) cross were used. Field trials were performed using a 10 × 10 square
lattice design with three replications. Data from 62 SSR markers were used for linkage analysis. The locations of the QTLs
on the linkage groups were determined by composite interval mapping method and the phenotypic variance explained by each marker
was determined by regression analysis. Several QTLs associated to disease resistance were identified in the population BS03 × BS04.
Some QTLs showed significant effects over the different environments studied. The existence of significant QTLs in common
among different environments indicates these genomic regions as possible new tools for marker-assisted selection in maize
breeding programs. 相似文献
7.
Jorge Luis Fuentes Fernando José Correa-Victoria Fabio Escobar Gustavo Prado Girlena Aricapa Myriam Cristina Duque Joe Tohme 《Euphytica》2008,160(3):295-304
The present work was conducted to identify microsatellite markers linked to the rice blast resistance gene Pi-1(t) for a marker-assisted selection program. Twenty-four primer pairs corresponding to 19 microsatellite loci were selected from
the Gramene database (www. gramene.org) considering their relative proximity to Pi-1(t) gene in the current rice genetic map. Progenitors and DNA bulks of resistant and susceptible families from F3 segregating populations of a cross between the near-isogenic lines C101LAC (resistant) and C101A51 (susceptible) were used
to identify polymorphic microsatellite markers associated to this gene through bulked segregant analysis. Putative molecular
markers linked to the blast resistance gene Pi-1(t) were then used on the whole progeny for linkage analysis. Additionally, the diagnostic potential of the microsatellite markers
associated to the resistance gene was also evaluated on 17 rice varieties planted in Latin America by amplification of the
specific resistant alleles for the gene in each genotype. Comparing with greenhouse phenotypic evaluations for blast resistance,
the usefulness of the highly linked microsatellite markers to identify resistant rice genotypes was evaluated. As expected,
the phenotypic segregation in the F3 generation agreed to the expected segregation ratio for a single gene model. Of the 24 microsatellite sequences tested, six
resulted polymorphic and linked to the gene. Two markers (RM1233*I and RM224) mapped in the same position (0.0 cM) with the
Pi-1(t) gene. Other three markers corresponding to the same genetic locus were located at 18.5 cM above the resistance gene, while
another marker was positioned at 23.8 cM below the gene. Microsatellite analysis on elite rice varieties with different genetic
background showed that all known sources of blast resistance included in this study carry the specific Pi-1(t) allele. Results are discussed considering the potential utility of the microsatellite markers found, for MAS in rice breeding
programs aiming at developing rice varieties with durable blast resistance based on a combination of resistance genes.
Centro Internactional de Agricultura Tropical (CIAT) institute where the research was carried out 相似文献
8.
Molecular mapping and identification of QTL's associated to oat crown rust partial resistance 总被引：1，自引：0，他引：1
Marta M. Barbosa Luiz C. Federizzi Sandra C. K. Milach José A. Martinelli Gladis C. Thomé 《Euphytica》2006,150(1-2):257-269
Summary Molecular mapping is a promising strategy for studying and understanding traits with complex genetic control, such as partial resistance to oat crown rust. The objectives of this research were to develop molecular maps from the progenies of the cross UFRGS7 (susceptible) × UFRGS910906 (partially resistant) and to identify QTLs (quantitative trait loci) associated to partial resistance to oat crown rust in two generations of that population.DNA of 86 genotypes of the F2 and 90 genotypes of the F6 UFRGS7 × UFRGS910906 population were used to generate AFLP markers. Molecular maps were constructed using Mapmaker Exp. 3.0 and QTLs for partial resistance to oat crown rust were identified with Mapmaker/QTL software. Five hundred and fifty seven markers in the F2 and 243 markers in the F6 generations were identified. The F2 map integrated 250 markers in 37 linkage groups. The F6 map integrated 86 markers in 17 linkage groups.Five QTLs were identified for partial resistance to oat crown rust in the F2 generation and three QTLs in the F6. The QTL identified on F6 through the PaaaMctt340 AFLP marker showed consistency across two environments and two generations (F4 and F6), and appear to have potential for marker-assisted selection in oat. 相似文献
9.
由菜豆炭疽菌引起的菜豆炭疽病是危害我国菜豆生产的主要病害之一, 鉴定和发掘新的抗病基因对于菜豆抗病育种具有十分重要的意义。以来自安第斯基因库的我国菜豆抗炭疽病地方品种红花芸豆与感病地方品种京豆杂交的F2群体为试验材料, 通过人工接种菜豆炭疽菌81号小种进行抗病性鉴定, 发现该分离群体中抗病植株数与感病植株数符合3∶1的分离比例, 确定红花芸豆对菜豆炭疽菌81号小种的抗性由显性单基因控制, 将此基因命名为Co-F2533。用分离群体分组分析法(BSA)和微卫星多态性分析(SSR)技术对红花芸豆中的抗炭疽病基因进行分子标记鉴定, 用Mapmaker3.0计算标记与目的基因间的遗传距离, 发现B6连锁群上的4个SSR标记BM170、Clon1429、BMD37、Clon410与抗炭疽病基因Co-F2533连锁, 遗传距离分别为6.6、18.4、20.9和30.9 cM, 这些SSR标记与Co-F2533基因在B6连锁群上的排列顺序为Clon1429-Co-F2533- BM170-BMD37-Clon410。根据基因所在连锁群的位置、抗病基因的基因库来源可知Co-F2533是一个新的来源于安第斯基因库的抗炭疽病基因。 相似文献
10.
Identification and characterization of RAPD and SCAR markers linked to anthracnose resistance gene in sorghum [Sorghum bicolor (L.) Moench] 总被引：1，自引：0，他引：1
Anthracnose, one of the destructive foliar diseases of sorghum growing in warm humid regions, is incited by the fungus Colletotrichum graminicola.The inheritance of anthracnose resistance was studied using the parental cultivars of Sorghum bicolor (L.) Moench, HC 136 (susceptible to anthracnose) and G 73 (anthracnose resistant). The F1 and F2 plants were inoculated with the local isolates of C. graminicola cultures. The F2 plants showed a segregation ratio of 3 (susceptible): 1(resistant) indicating that the locus for resistance to anthracnose in sorghum accession G 73 segregates as a recessive trait in a cross to susceptible cultivar HC 136. RAPD (random amplified polymorphic DNA) marker OPJ 011437 was identified as marker closely linked to anthracnose resistance gene in sorghum by bulked segregant analysis of HC 136 × G73 derived recombinant inbred lines (RILs) of sorghum. A total of 84 random decamer primers were used to screen polymorphism among the parental genotypes. Among these, only 24 primers were polymorphic. On bulked segregant analysis, primer OPJ 01 amplified a 1437 bp fragment only in resistant parent G 73 and resistant bulk. The marker OPJ 011437 was cloned and sequenced. The sequence of RAPD marker OPJ 011437 was used to generate specific markers called sequence characterized amplified regions (SCARs). A pair of SCAR markers SCJ 01-1 and SCJ 01-2 was developed using Mac Vector program. SCAR amplification of resistant and susceptible parents along with their respective bulks and RILs confirmed that SCAR marker SCJ 01 is at the same loci as that of RAPD marker OPJ 011437 and hence, is linked to anthracnose resistance gene. Resistant parent G 73 and resistant bulk amplified single specific band on PCR amplification using SCAR primer pairs. The RAPD marker OPJ 011437 was mapped at a distance of 3.26 cM apart from the locus governing anthracnose resistance on the sorghum genetic map by the segregation analysis of the RILs. Using BLAST program, it was found that the marker showed 100 per cent alignment with the contig{_}3966 located on the longer arm of chromosome 8 of sorghum genome. Therefore, these identified RAPD and SCAR markers can be used in the resistance-breeding program of sorghum anthracnose by marker-assisted selection.An erratum to this article can be found at 相似文献
11.
Giovani Greigh de Brito Eveline Teixeira Caixeta Ana Paula Gallina Eunize Maciel Zambolim Laércio Zambolim Valdir Diola Marcelo Elhers Loureiro 《Euphytica》2010,173(2):255-264
The most important disease of Coffea arabica is coffee leaf rust caused by the fungus Hemileia vastatrix. The purpose of this study was to characterize the inheritance of coffee resistance gene(s) to race II of this pathogen and
to identify and map molecular markers linked to this trait. Different populations were used: F2 (160 plants), BCr (20), and BCs (135), derived from a cross between the resistant genotype Híbrido de Timor UFV 427-15 and
the susceptible cultivar Catuaí Amarelo UFV 2143-236 (IAC 30). The segregation analysis showed that the resistance of Híbrido
de Timor to race II of the H. vastatrix is conferred by a single dominant gene. The amplification of 176 AFLP (Amplified fragment length polymorphism) primer combinations
using bulked segregant analysis (BSA) allowed the identification of three molecular markers linked to the resistance gene.
Genetic mapping of these three markers in the F2 population indicated that they are distributed on both sides, flanking the resistance gene. The markers E.CTC/M.TTT405 and
E.CGT/M.TGT300 were found linked to the resistance gene at 8.69 cM (LOD 18.91) and 25.10 cM (LOD 5.37), respectively, while
E.CCT/M.TTC230 was localized on the other side of the gene, at 20.50 cM (LOD 6.15). These markers are the first rust resistance
markers identified in Híbrido de Timor and can be useful for marker assisted selection in coffee breeding programs. 相似文献
12.
Random amplified polymorphic DNA (RAPD) was used with the objective of identifying DNA markers linked to the sclerotinia crown and stem rot (SCSR) resistance of red clover. Bulked segregant analysis was used to detect polymorphism that should be linked to SCSR resistance. Two bulks were made by pooling previously extracted DNA. Each bulk (one resistant, and the other susceptible) consisted of eight genotypes from an F2 population obtained from a cross between a susceptible and a resistant parent. A binomial model was used to select RAPD fragments with a low probability of no linkage with SCSR resistance. Four RAPD fragments were retained as candidate markers of SCSR resistance. Three are associated with resistance and one with susceptibility. 相似文献
13.
Fábio Gelape Faleiro Vagner Tibaldi Queiroz Uilson Vanderlei Lopes Cláudia Teixeira Guimarães José Luis Pires Milton Macoto Yamada Ioná Santos Araújo Messias Gonzaga Pereira Raymond Schnell Gonçalo Apolinário de Souza Filho Cláudia Fortes Ferreira Everaldo Gonçalves Barros Maurílio Alves Moreira 《Euphytica》2006,149(1-2):227-235
Molecular markers (RAPD, AFLP and microsatellites) were used to generate a linkage map and to identify QTLs associated to witches' broom (Crinipellis perniciosa) resistance in cacao (Theobroma cacao), using 82 individuals of an F2 population derived from the clones ICS-1 (susceptible) and Scavina-6 (resistant). Fifteen evaluations of the number of brooms have been carried out in six years (1997–2002). In order to increase the precision and accuracy in the measures of resistance, each F2 plant was cloned in three replications in a randomized block design with single-tree plots and evaluated over 2 years. Three hundred and forty-two markers were obtained, being 33 microsatellites, 77 AFLPs and 232 RAPDs. The distribution of the number of brooms in the F2 population was skewed to resistance, suggesting the involvement of major genes controlling resistance and the repeatability estimated for resistance was 44%. A strong putative QTL was detected as being related to witches' broom resistance. Associated to this QTL, the microsatellite mTcCIR35 explained 35.5% of the phenotypic variation in resistance. This marker is being used for marker-assisted selection in Scavina-6 progenies, including those selected in private plantations, as an auxiliary tool to the phenotypic selection. 相似文献
14.
Bulked segregant analysis was employed to identify random amplified polymorphic DNA (RAPD) markers linked to a gene that confers rhizomania resistance to a sugar beet line created from a Holly Sugar Company breeding population (USA). Polymorphism revealed with 160 arbitrary 10-mer oligonucleotide primers was screened in two bulks produced by separately pooling the individual DNAs from the six most resistant and the six most susceptible plants of an F2 population segregating for rhizomania resistance. A study of the F2 individuals showed that 19 primers generated 44 polymorphic markers which were then grouped into nine linkage groups. By analysis of variance, 12 were shown to have a significant effect upon the level of resistance and were mapped on a segment 22.3 cM long. A quantitative trait locus (QTL) of resistance was identified and located in a 4.6cM interval between two markers. It accounted for 67.4% of the observed variation and almost all the genetic variation. These results suggest that the identified QTL corresponds to a unique major gene conditioning the Holly resistance studied, which we have named Rz-l. 相似文献
15.
R. Amiri M. Moghaddam M. Mesbah S. Yaghoub Sadeghian M.R. Ghannadha K. Izadpanah 《Euphytica》2003,132(3):363-373
In this study, the inheritance of resistance to Beet necrotic yellow vein virus (BNYVV) in accessions Holly-1-4and WB42 was investigated. Crosses between both resistant sources and susceptible parents
were carried out and F1F2 and BC1 populations were obtained. Virus concentrations in WB42and its F1 populations were lower than in Holly-1-4. Observed ratios of susceptible and resistant plants in segregating populations
of Holly-1-4 as well as WB42 were in agreement with hypothesis of one dominant major gene. Segregation of plants in F2 populations obtained from crosses betweenHolly-1-4 and WB42 revealed that the resistance genes in Holly-1-4 and WB42 were
nonallelic and linked loci.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
16.
Molecular mapping of a novel blast resistance gene Pi38 in rice using SSLP and AFLP markers 总被引：2，自引：0，他引：2
Blast, caused by Magnaporthe grisea, is the most devastating disease of rice worldwide. In this study, the main objective was to identify and map a new gene for blast resistance, in an indica rice cultivar ‘Tadukan’ against blast fungal isolate B157, using molecular tools. F2 segregating population was derived from ‘CO39’ (susceptible) and ‘Tadukan’ (resistant), and molecular mapping of the blast resistance gene was carried out using simple sequence length polymorphism (SSLP) and amplified fragment length polymorphism (AFLP) methods. Two SSLP markers, RM206 and RM21 and three AFLP markers (AF1: E‐aca/M‐ctt; AF2: E‐aca/M‐cat and AF3: E‐acc/M‐cac2) were identified to be linked to the resistance gene. The co‐segregation analysis using SSLP markers implied that the blast resistance gene designated Pi38 resides on rice chromosome 11. 相似文献
17.
Fusarium wilt caused by Fusarium oxysporum Schlechtend.: Fr f. sp. ciceris (Padwick) Matuo & Sato is a devastating disease of chickpea. The current study was conducted to determine the inheritance
of the gene(s) for resistance to race 4 of fusarium wilt and to identify linked RAPD markers using an early wilting line,
JG-62, as a susceptible parent. Genetic analysis was performed on the F1s, F2s and F3 families from the cross of JG-62 × Surutato-77. The F3 families were inoculated with a spore suspension of the race 4 wilt pathogen and the results were used to infer the genotypes
of the parent F2 plants. Results indicated that two independent genes controlled resistance to race 4. Linkage analysis of candidate RAPD
marker, CS-27700, and the inferred F2 phenotypic data showed that this marker locus is linked to one of the resistance genes. Allelism indicated that the two resistance
sources, Surutato-77 and WR-315, shared common alleles for resistance and the two susceptible genotypes, C-104 and JG-62,
carried alleles for susceptibility. The PCR-based marker, CS-27700, was previously reported to be linked to the gene for resistance to race 1 in a different population which suggested that
the genes for resistance to races 1 and 4 are in close proximity in the Cicer genome.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
18.
Black rot is the most devastating disease of cauliflower worldwide causing severe damage to crop. The identification of markers linked to loci that control resistance can facilitate selection of plants for breeding programmes. In the present investigation, F2 population derived from a cross between ‘Pusa Himjyoti’, a susceptible genotype, and ‘BR‐161’, a resistant genotype, was phenotyped by artificial inoculation using Xcc race 1. Segregation analysis of F2 progeny indicated that a single dominant locus governed resistance to Xcc race 1 in ‘BR‐161’. Bulk segregant analysis in resistant and susceptible bulks of F2 progeny revealed seven differentiating polymorphic markers (three RAPD, two ISSR and two SSR) of 102 markers screened. Subsequently, these markers were used to genotype the entire F2 population, and a genetic linkage map covering 74.7 cM distance was developed. The major locus Xca1bo was mapped in 1.6‐cM interval flanked by the markers RAPD 04833 and ISSR 11635. The Xca1bo locus was located on chromosome 3. The linked markers will be useful for marker‐assisted resistance breeding in cauliflower. 相似文献
19.
K.K. Jena I.C. Pasalu Y.K. Rao Y. Varalaxmi K. Krishnaiah G.S. Khush G. Kochert 《Euphytica》2003,129(1):81-88
An introgression line derived from an interspecific cross between Oryzasativa and Oryza officinalis, IR54741-3-21-22 was found to beresistant to an Indian biotype of brown planthopper (BPH). Genetic analysisof 95 F3 progeny rows of a cross between the resistant lineIR54741-3-21-22 and a BPH susceptible line revealed that resistance wascontrolled by a single dominant gene. A comprehensive RAPD analysisusing 275 decamer primers revealed a low level of (7.1%) polymorphismbetween the parents.RAPD polymorphisms were either co-dominant (6.9%), dominant forresistant parental fragments (9.1%) or dominant for susceptible parentalfragments (11.6%). Of the 19 co-dominant markers, one primer,OPA16, amplified a resistant parental band in the resistant bulk and asusceptible parental band in the susceptible bulk by bulked segregantanalysis. RAPD analysis of individual F2 plants with the primerOPA16 showed marker-phenotype co-segregation for all, with only onerecombinant being identified. The linkage between the RAPD markerOPA16938 and the BPH resistance gene was 0.52 cM in couplingphase. The 938 bp RAPD amplicon was cloned and used as a probe on122 Cla I digested doubled haploid (DH) plants from aIR64xAzucena mapping population for RFLP inheritance analysis and wasmapped onto rice chromosome 11. The OPA16938 RAPD markercould be used in a cost effective way for marker-assisted selection of BPHresistant rice genotypes in rice breeding programs. 相似文献
20.
Mapping and validation of QTLs for resistance to an Indian isolate of Ascochyta blight pathogen in chickpea 总被引：1，自引：0，他引：1
Pratibha Kottapalli Pooran M. Gaur Sanjay K. Katiyar Jonathan H. Crouch Hutokshi K. Buhariwalla Suresh Pande Kishore K. Gali 《Euphytica》2009,165(1):79-88
Ascochyta blight (AB) caused by Ascochyta rabiei, is globally the most important foliar disease that limits the productivity of chickpea (Cicer arietinum L.). An intraspecific linkage map of cultivated chickpea was constructed using an F2 population derived from a cross between an AB susceptible parent ICC 4991 (Pb 7) and an AB resistant parent ICCV 04516. The
resultant map consisted of 82 simple sequence repeat (SSR) markers and 2 expressed sequence tag (EST) markers covering 10
linkage groups, spanning a distance of 724.4 cM with an average marker density of 1 marker per 8.6 cM. Three quantitative
trait loci (QTLs) were identified that contributed to resistance to an Indian isolate of AB, based on the seedling and adult
plant reaction. QTL1 was mapped to LG3 linked to marker TR58 and explained 18.6% of the phenotypic variance (R
2) for AB resistance at the adult plant stage. QTL2 and QTL3 were both mapped to LG4 close to four SSR markers and accounted
for 7.7% and 9.3%, respectively, of the total phenotypic variance for AB resistance at seedling stage. The SSR markers which
flanked the AB QTLs were validated in a half-sib population derived from the same resistant parent ICCV 04516. Markers TA146
and TR20, linked to QTL2 were shown to be significantly associated with AB resistance at the seedling stage in this half-sib
population. The markers linked to these QTLs can be utilized in marker-assisted breeding for AB resistance in chickpea. 相似文献