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1.
Sugarcane (Saccharum spp) is an important crop for both sugar and biofuel production. However, the sugarcane breeding process has resulted in modern sugarcane cultivars with a narrow genetic basis. To broaden the genetic basis and promote international collaborations in sugarcane cultivar development, we documented the peidgrees of representative sugarcane culativars widely used in China and the United States of America (USA), recruited more than six thousand simple sequence repeat (SSR) markers for sugarcane, and assessed the genetic diversity and relationships beween representative sugarcane cultivars and their potential ancestry accessions. The SSR gentoyping results indicated that both the USA and Chiniese cultivars had low genetic diversity, specifically the Chinese cultivars. The USA sugarcane cultivars experienced high presure of selection for sugar content as they had the closest relationship with S. officinarum, followed by Chinese cultivars, S. robustum, and S. spontaneum. The sugarcane accessions assessed could be divided into five and four groups through cluster and principal component analysis, respectively. S. spontaneum as a potential ancestor contributing to the stress tolerance of sugarcane cultivars was grouped into distinct clusters, and S. officinarum was grouped with sugarcane cultivars in both countries. S. robustum did not seem to contribute to the sugarcane cultivar development in China, but may have contributed to the USA cultivar development. This study not only provided a collection of easy to use SSR markers, but also detailed genetic diversity and relationship among the cultivars in the two counties, which will be referable to promote international collaboration and broaden the genetic basis of sugarcane cultivars.  相似文献   

2.
The effect of elevated tropospheric ozone concentration [O3] on root processes in wheat systems of different O3 sensitivity is not well understood. Two wheat cultivars (cv. Y15 and YN19) with contrasting O3 tolerance were grown in a fully open‐air O3 enrichment platform for one season. We found that elevated O3 (EO3) (50% above the ambient O3) significantly decreased the total biomass at all key growth stages and the yield of the O3‐sensitive cultivar YN19 but did not affect those of the O3‐tolerant cultivar Y15. EO3 significantly decreased the root biomass of two wheat cultivars at the jointing and grain‐filling stages. EO3 significantly decreased the root length, length density, surface area and volume of the two cultivars at the jointing stage but increased those of YN19 at the grain‐filling stage. EO3 significantly increased the root activities (specific root respiration rates) of Y15 and YN19 at the jointing, heading and grain‐filling stages. EO3 significantly decreased the contribution of fresh root respiration to soil respiration (CRS) of YN19 at the jointing stage but increased it at the heading stage; however, it did not change the CRS of Y15 at any growth stages. This study indicates that the effects of EO3 on root morphology and activity varied among wheat cultivars, and suggest that we can breed O3‐tolerant cultivars to maintain crop yield under higher [O3] scenarios.  相似文献   

3.
小麦、玉米粒重动态共性特征及其最佳模型的筛选与应用   总被引：4，自引：0，他引：4  
为了定量描述小麦、玉米两作物粒重变化共性特征,选用3个冬小麦和4个夏玉米不同熟期型品种进行不同密度的田间试验,对其粒重(GW)动态进行测定,并对GW和灌浆天数进行“归一化”处理得到的3个主要模型进行比较,结果表明,Logistic曲线方程y= a/(1+be–cx) 具有广泛适应性和生物学意义,具体方程式为y=1.0624/(1+52.8653e–6.7609x),r=0.9916 (P<0.01)。不同作物、品种、密度处理的方程参数a值基本为1;参数b在密度间变异很小,品种间变幅较大,为45.3379~66.9306;c值在品种和密度间变异均很小,在小麦和玉米间的变幅分别为6.2122~6.8025和7.0199~7.7325。应用本试验及河南焦作高产冬小麦和山东泰安高产夏玉米不同品种的GW试验资料对模型分别进行验证表明,冬小麦和夏玉米的归一化GW动态共性模型的模拟准确度(以k表示),分别为0.9870、1.0057和0.9982、1.0131,精确度(以R2表示)分别为0.9854、0.9918和0.9772、0.9926。说明归一化方法建立的小麦、玉米GW动态共性模型能够准确地反映两作物GW动态共性变化特点。利用该模型,仅根据品种的灌浆期和最大GW,以及参数b值的品种特点,便可还原整个灌浆期的GW动态。计算不同地点、年份及不同品种、密度处理的冬小麦、夏玉米灌浆前、中、后期的GW模拟值与测量值均比较接近,误差小于0.2797。  相似文献   

4.
Assessments of impacts of future climate change on widely grown sugarcane varieties can guide decision‐making and help ensure the economic stability of numerous rural households. This study assessed the potential impact of future climatic change on sugarcane grown under dryland conditions in Mexico and identified key climate factors influencing yield. The Agricultural Land Management Alternatives with Numerical Assessment Criteria (ALMANAC) model was used to simulate sugarcane growth and yield under current and future climate conditions. Management, soil and climate data from farm sites in Jalisco (Pacific Mexico) and San Luis Potosi (Northeastern Mexico) were used to simulate baseline yields. Baseline climate was developed with 30‐year historical data from weather stations close to the sites. Future climate for three decadal periods (2021–2050) was constructed by adding forecasted climate values from downscaled outputs of global circulation models to baseline values. Climate change impacts were assessed by comparing baseline yields with those in future decades under the A2 scenario. Results indicate positive impacts of future climate change on sugarcane yields in the two regions, with increases of 1%–13% (0.6–8.0 Mg/ha). As seen in the multiple correlation analysis, evapotranspiration explains 77% of the future sugarcane yield in the Pacific Region, while evapotranspiration and number of water and temperature stress days account for 97% of the future yield in the Northeastern Region. The midsummer drought (canicula) in the Pacific Region is expected to be more intense and will reduce above‐ground biomass by 5%–13% (0.5–1.7 Mg/ha) in July–August. Harvest may be advanced by 1–2 months in the two regions to achieve increases in yield and avoid early flowering that could cause sucrose loss of 0.49 Mg ha?1 month?1. Integrating the simulation of pest and diseases under climate change in crop modelling may help fine‐tune yield forecasting.  相似文献   

5.
Cultivar pedigrees from two sugarcane origins, 9 Argentine (AR) and 7 American (AM) have been reconstructed, and their genetic similarities (based on coefficient of parentage, COP, estimates) show an average of 0.206 ± 0.054. CP clones that enter the pedigrees of AM cultivars are parents or grandparents of AR cultivars, demonstrating that these genotypes have a strong genetic lineage in common. On average, AR pedigrees are smaller and contain less number of founding species than AM pedigrees. However, the lower height of the former is not explained by the different participation of founding species in the pedigrees. The presence of founding species in AR and AM pedigrees increases with the year of cultivar selection, indicating that more founding species entered the pedigree in recently selected cultivars than in older ones. The ancestry of the 16 cultivars trace back to 3 founding species: Saccharum officinarum, S. barberi, and S. spontaneum, with S. officinarum showing the greatest percentage of participation. As S. sinensis participates in 13 pedigrees and S. robustum in 3, the 5 founding species are present in 2 out of 16 pedigrees. Interestingly, the genus Sorghum enters the pedigree of LCP 85-384 pedigree. Industrial parameters assayed indicate that AR and AM cultivars were preferentially selected for their relatively high sucrose content and sugar yield in accordance with two facts: (i) the genetic background and the sucrose genes of sugarcane hybrids provided by the 68% of participation S. officinarum clones, and (ii) no clones of high fiber S. spontaneum have entered the pedigrees in earlier generations.  相似文献   

6.
Biophysical models to simulate crop yield are increasingly applied in regional climate impact assessments. When performing large-area simulations, there is often a paucity of data to spatially represent changes in genotype (G) and management (M) across different environments (E). The importance of this uncertainty source in simulation results is currently unclear. In this study, we used a variance-based sensitivity analysis to quantify the relative contribution of maize hybrid (i.e. G) and sowing date (i.e. M) to the variability in biomass yield (YT, total above-ground biomass) and harvest index (HI, fraction of grain in total yield) of irrigated silage maize, across the extent of arable lands in New Zealand (i.e. E). Using a locally calibrated crop model (APSIM-maize), 25 G x M scenarios were simulated at a 5 arc minute resolution (∼5 km grid cell) using 30 years of historical weather data. Our results indicate that the impact of limited knowledge on G and M parameters depends on E and differs between model outputs. Specifically, the sensitivity of YT and HI to genotype and sowing date combinations showed different patterns across locations. The absolute impact of G and M factors was consistently greater in the colder southern regions of New Zealand. However, the relative share of total variability explained by each factor, the sensitivity index (Si), showed distinct spatial patterns for the two output variables. The YT was more sensitive than HI in the warmer northern regions where absolute variability was the smallest. These patterns were characterised by a systematic response of Si to environmental drivers. For example, the sensitivity of YT and HI to hybrid maturity consistently increased with temperature. For the irrigated conditions assumed in our study, inter-annual weather conditions explained a higher share of total variability in the southern colder regions. Our results suggest that the development of methods and datasets to more accurately represent spatio-temporal G and M variability can reduce uncertainty in regional modelling assessments at different degrees, depending on prevailing environmental conditions and the output variable of interest.  相似文献   

7.
Genetic improvement of sugar content in sugarcane would benefit from the availability of sufficient DNA markers and a genetic map. Genetic linkage maps were constructed to identify quantitative trait loci (QTLs) for seedling brix (SB), brix (B), sucrose percent in juice (SUC), stalk number (SN), stalk length (SL), stalk diameter (SD), internodes (INT), number of green leaves (NGL), at three crop cycles across seven environments in a segregating population with 207 individuals derived from a bi-parental cross of sugarcane elite cultivars. Linkage analysis led to the construction of eight linkage groups (LGs) for Co86011 and sixteen LGs for CoH70. The combined length of the two linkage maps was 2606.77 cM distributed over 24 LGs. 31 QTLs were identified: 2 for SB, 7 for B, 6 for SUC, 4 for SN, 1 for SL, 3 for SD, 6 for INT and 2 for NGL at LOD scores ranging from 2.69 to 4.75. 7 QTLs (22 %) had stable effect across crop year and locations. Markers from parents were found to be associated with both positive and negative effect on all of the traits analyzed. The most important QTLs intervals identified in this study using single-dose marker, were qB2, qSUC2, qINT2 and qB2, qSUC2, qSL2, qINT2 located between SSR markers UGSM31548 and UGSM31649. These QTLs could be put into use in marker assisted breeding.  相似文献   

8.
The crop growth is highly dependent on growth conditions which vary from year to year making precision farming challenging. In the present paper was first investigated whether varying soil physical properties reflect the within-field yield variation of small grain cereals and how do the variations in weather conditions between growing seasons affect the within-field yield variation. Secondly, the potential biomass accumulation of the crop in existing soil and weather conditions was simulated. The simulated and experimentally based site-specific total biomasses were compared in order to find out whether the soil data explains the observed variations in yield.Three experimental fields size of 3–4 ha were established to examine the spatio-temporal yield variation during three years. The clay content of soils was high (> 46%) and soils were classified as Stagni-Vertic Cambisols. Correlations between soil water retention properties and crop yield were studied. Top and subsoil saturated (SWC), field capacity (FC) and permanent wilting point (PWP) water content, and saturated hydraulic conductivity of soil (Ksat), were determined from 19 to 24 places on each field once during the three years experimental period. During growing seasons, soil moisture content and leaf area index (LAI) were determined at same places biweekly, and yield was harvested. Spring barley (Hordeum vulgare) was grown on two fields, and spring wheat (Triticum aestivum, 2 years) and spring oilseed rape (Brassica napus L., one year) were grown on the third field.The measured grain yields correlated with selected soil physical properties only in few cases. The observed spatial variation in the biomass was in most cases found to be higher than the simulated. Therefore, the above mentioned parameters were not enough to predict the yield correctly in case of high variations. There were other factors decreasing the observed yield e.g. lodging, cold summer, extremely high precipitation and slopes in field. According to our results it is evident that it is very difficult to predict site-specific biomass accumulation solely by soil properties in order, for instance, to fertilize in a site-specific manner. Therefore one needs to measure the crop during the growing season in order to simulate the biomass accumulation for precision farming purposes.  相似文献   

9.
Summary With the increasing rate of new cultivar production for different crop plants, there is great need for methods of identifying each cultivar discriminatingly. Starch gel electrophoresis was employed to study the differences between the esterase and cathodal peroxidase isozyme patterns of 40 broad bean (Vicia faba L.) cultivars. A total of 10 and 17 medium to darkly stained bands were obtained for esterase and peroxidase systems, respectively. Bands from each enzyme system could be gropuped into three zones. Bands belonging to zone 1 of esterase (E1) and zones 2 and 3 of peroxidase (P2 and P3) were quite distinct, stained intensely, and were especially useful for identification purposes.The differences in banding patterns among cultivars of the same origin were as great as those of cultivars of unrelated origin. A large proportion of the cultivars could be completely differentiated using both of the isozyme systems. There were no bands present in either enzyme system which were common to all cultivars.  相似文献   

10.
Nitrogen use efficiency (NUE) in cultivated rice has become of utmost importance due to ecological and economical consequences of nitrogen (N) losses. However, detailed information on the components of NUE—physiological, absorption and agronomical NUEs—is lacking. The present study aimed to determine the components of NUE for three cultivars of Asian and African rices [IR64 (Oryza sativa indica), Azucena (O. sativa japonica) and TOG7105 (O. glaberrima)] exposed to a wide range of N supply, from excess to deficiency—from 4× to 1/8× of the standard Yoshida solution (1×). Increasing the N supply to 2× or 4× did not induce much change for most parameters, including tissue N concentrations and pNUE. However, aNUE and agNUE decreased gradually as the N supply increased from 1/2× to 4×. In contrast, lowering the N supply, particularly to 1/4× or 1/8×, induced a significant decrease in most measured parameters, except NUEs. The pNUE increased gradually when lowering the N supply from 1× to 1/8×, while aNUE and agNUE were maximal at 1/2 and/or 1/4× according to the cultivar. In contrast, the Fv/Fm and PSII values remained unchanged. Differences between cultivars were low. However, the O. glaberrima cultivar showed a significantly lower aNUE and agNUE than both O. sativa cultivars under low N supply (1/4 and 1/8×). These results demonstrate that all NUE components were sharply and differentially affected by low N supply, while the PSII remained unaffected. These results are important for determining the cultivar and N supply with the best balance between intensive and sustainable rice cultivation.  相似文献   

11.
Cereal cultivars conferring a high degree of crop competitive ability, especially against aggressive weeds, are highly beneficial in organic farming as well as other farming systems that aim to limit the use of herbicides. In this study, thirteen winter wheat cultivars, plus one spring wheat and one winter oat were assessed for their competitive ability at key growth stages, across three seasons. The natural population of weed species was allowed develop without agronomic intervention. Weed suppression ability for each cultivar (S var) was calculated as the difference between weed growth in plots for each cultivar and the maximal weed growth (W max) from adjacent uncropped areas. The sensitivity of S var in response to changes in weed growth (S varW) was derived from the linear regression coefficient of S var plotted against W max. There was significant variation in S var between cultivars and strong evidence for cultivars to vary in S varW. Amongst groups of cultivars with similar levels of S var some could be defined as being of higher or lower sensitivity to changes in weed growth. Some cultivars also had relatively good S var at high levels of weed growth. The use of both weed suppression ability and sensitivity across different levels of weed growth or weed populations has considerable potential for selecting new cultivars suitable for organic agriculture. Ideally new cultivars will be selected on the basis of high S var and/or low S varW. This analysis provides the means to measure sensitivity of cultivar performance across a range of favourable and unfavourable conditions.  相似文献   

12.
Location specific adaptation option is required to minimize adverse impact of climate change on rice production. In the present investigation, we calibrated genotype coefficients of four cultivars in the CERES-Rice model for simulation of rice yield under elevated CO2 environment and evaluation of the cultivar adaptation in subtropical India. The four cultivars (IR 36, Swarna, Swarn sub1, and Badshabhog) were grown in open field and in Open Top Chamber (OTC) of ambient CO2 (≈390 ppm) and elevated CO2 environment (25% higher than the ambient) during wet season (June–November) of the years 2011 and 2012 at Kharagpur, India. The genotype coefficients; P1 (basic vegetative phase), P2R (photoperiod sensitivity) and P5 (grain filling phase) were higher, but G1 (potential spikelet number) was lower under the elevated CO2 environment as compared to their open field value in all the four cultivars. Use of the calibrated model of elevated CO2 environment simulated the changes in grain yield of −13%, −17%, −4%, and +7% for the cultivars IR 36, Swarna, Swarna sub1, and Badshabhog, respectively, with increasing CO2 level of 100 ppm and rising temperature of 1 °C as compared to the ambient CO2 level and temperature and they were comparable with observed yield changes from the OTC experiment. Potential impacts of climate change were simulated for climate change scenarios developed from HadCM3 global climate model under the Intergovernmental Panel on Climate Change Special Report on Emission Scenarios (A2 and B2) for the years 2020, 2050, and 2080. Use of the future climate data simulated a continuous decline in rice grain yield from present years to the years 2020, 2050 and 2080 for the cultivars IR 36 and Swarna in A2 as well as B2 scenario with rising temperature of ≥0.8 °C. Whereas, the cultivar Swarna sub1 was least affected and Badshabhog was favoured under elevated CO2 with rising temperature up to 2 °C in the sub-tropical climate of India.  相似文献   

13.
Low erucic acid (LEA) rapeseed, which has accumulated mutant fatty acid elongase genes at the BnFAE1.1 and BnFAE1.2 loci of the A- and C-genome, respectively, is an important oilseed crop. Short growing turnip rape (B. rapa) is also important as a catch crop in the continuous cropping of rice in Asia but there is no LEA B. rapa cultivar for cultivation in South Asia. In order to develop LEA turnip rape cultivars, high erucic acid turnip rape cultivars were interspecifically crossed as recurrent parents to a canola quality rapeseed. In the meantime, we monitored incorporation of the mutant bnfae1.1 (e1) gene into A-genome of turnip rape, using a dCAPS primer pair, which can amplify PCR fragment only for the mutant e1 gene from A-genome. The early backcross progenies showed poor seed set, but which was improved in advanced progenies. Finally, homozygous e1e1 genotypes were established in the selfed progenies of BC2–BC3, and their LEA content was confirmed by gas-chromatography analysis. Our results and promising lines will contribute to LEA-trait selection in turnip rape and rapeseed breeding.  相似文献   

14.
The aim was to study the growth and development of six spring barley (Hordeum vulgare ssp. vulgare L.) cultivars as a response to a model weed population of Sinapis alba L. The development of light interception profiles over time was characterized for each cultivar in weed‐free stands. The cultivars were chosen such that they represent a range of weed‐suppressive abilities based on previously performed trials. One field experiment each was conducted in 1996 and 1997 at a site SE of Uppsala, Sweden. The two cultivars with low competitive ability against weeds, Etna and Blenheim, allowed the highest weed biomass and the lowest crop biomass in competition with the weeds. These two cultivars transmitted more photosynthetic active radiation through the canopy down to 20 and 40 cm height than did the other cultivars. Although the biomass of cv. Etna was low, the grain yield was higher than that of the other cultivars when grown in competition with weeds. In 1997, cv. Svani with good competitive ability against weeds transmitted least light and had greater grain yield than most other cultivars. The absence of a relationship between high grain yield and low weed suppressive ability in the present study indicates that it should be possible for plant breeders to combine high grain yielding capacity with approved weed‐competitive ability.  相似文献   

15.
Tomato cultivars differ in their sensitivity to heat stress, and the sensitivity depends on the developmental stage of the plants. It is less known how heat stress affects tomato at the anthesis stage in terms of leaf physiology and fruit set and whether the ability of tomato to tolerate heat at different developmental stages is linked. To investigate photosynthetic gas exchange characteristics, carbohydrate content and fruit set during heat stress, a thermo‐tolerant cultivar (‘LA1994’) and a thermo‐sensitive cultivar (‘Aromata’) were studied at the seedling and anthesis stage. The photosynthetic parameters, maximum quantum efficiency of photosystem II (Fv/Fm), chlorophyll content, carbohydrate content and fruit set were determined in plants grown at 26/18 °C (control) and 36/28 °C (heat stress). The physiological responses including net photosynthetic rate (PN), chlorophyll content and Fv/Fm decreased in ‘Aromata’ at both developmental stages during heat stress, whereas they were unaltered in ‘LA1994’ during heat stress as compared to the respective control. This was accompanied by lower contents of glucose and fructose in mature leaves of ‘Aromata’ at the seedling stage under heat stress. In contrast, the glucose content increased while the fructose content was unaltered in mature leaves of ‘LA1994’ at the seedling stage under heat stress. High temperature induced a similar change in carbohydrate content in the young leaves of both cultivars at anthesis. The fructose and sucrose content were unaffected in the mature leaves of ‘Aromata’ but significantly increased in ‘LA1994’ under heat stress at anthesis. The heat stress treatment decreased pollen viability and inhibited fruit set due to flower wilting and abnormal abscission in ‘Aromata’, whereas fruit set was not inhibited in ‘LA1994’. A decrease in chlorophyll content, photosynthesis and carbohydrate content in the mature leaves of tomato could be related to fruit set failure at high temperature. The results show that physiological responses to heat stress at the seedling stage correspond with the responses at the anthesis stage, demonstrating that screening for heat stress sensitivity can be carried out in young plants.  相似文献   

16.
Previously we reported that postproduction quality of pot ‘Seadov’ tulip (Tulipa gesneriana) was significantly increased by GA4+7 plus BA in a manner dependent on the concentration and stage of flower development at application. In these experiments, we extended the survey to 20 tulip cultivars to further evaluate the effects of GA4+7 plus BA sprays for enhancing postproduction flower and leaf quality. The senescence symptom of the cultivars fell into three categories: wilting, wilting-abscission (abscission shortly after tepal wilting) and abscission (abscission without wilting), with the majority of the cultivars belonging to the wilting and wilting-abscission categories. Pots bearing six plants were sprayed with a range of GA4+7 plus BA concentrations at marketable stage and placed in a simulated consumer environment (SCE). GA4+7 plus BA significantly enhanced individual flower and postproduction longevity, but the effect was dependent upon the senescence category of the cultivar. In general, GA4+7 plus BA increased individual flower and postproduction longevity of wilting-type cultivars at concentrations above 10 mg L?1, while longevity of wilting-abscission-type cultivars was only enhanced by 50 mg L?1. Abscission-type cultivars were not affected by any concentrations of GA4+7 plus BA. Regardless of floral senescence category, leaf yellowing was significantly reduced by GA4+7 plus BA sprays in those cultivars showing postproduction leaf yellowing. GA4+7 plus BA did not induce leaf and stem elongation in most cultivars. Only ‘Yellow Baby’, the shortest cultivar, showed elongation of stem and leaf by GA4+7 plus BA at concentrations above 25 mg L?1. Spray applications of GA4+7 plus BA can be useful to enhance flower and leaf quality in pot tulips.  相似文献   

17.
The marked underutilization of pea ecotype and old cultivar genetic resources need be reversed by producing information on elite germplasm and diversity patterns for key agronomic traits. This study generated such information for a world collection of 223 ecotypes or old cultivars of P. sativum subsp. sativum subdivided into 19 regional pools (each represented by 9–15 entries) and one reference pool of 11 locally-adapted varieties from France, Spain and Germany. The collection was evaluated for grain yield, seed protein content, straw biomass, phenology and other agronomic and morphological traits in a spring-sown, water-limited environment of Northern Italy. Variation within ecotype/old cultivar germplasm pools was at least two-fold greater for seed size and protein content and seven-fold greater for other traits relative to variation between pools, and was particularly large within pools from Central Europe, Near East and United Kingdom. The traditional germplasm, compared with improved variety material, tended to display higher dry grain yield (1.11 vs. 0.85 t/ha) and straw biomass, later crop maturity, smaller seed, and similar protein content. Traditional germplasm from Central Europe combined outstanding grain yield and total biomass with high seed protein content. High grain yield characterized, inter alia, the germplasm pools from Ukraine and Spain, the latter also featuring high protein content. Several ecotype/old cultivar accessions out-performed (P < 0.05) the best-ranking varieties for grain yield or protein content. Patterns of phenotypic variation were poorly related to geographic origin of germplasm pools, while highlighting the distinctness of improved variety germplasm.  相似文献   

18.
Insertion-deletion (indel) polymorphisms, such as simple sequence repeats, have been widely used as DNA markers to identify QTLs and genes and to facilitate rice breeding. Recently, next-generation sequencing has produced deep sequences that allow genome-wide detection of indels. These polymorphisms can potentially be used to develop high-accuracy polymerase chain reaction (PCR)-based markers. Here, re-sequencing of 5 indica, 2 aus, and 3 tropical japonica cultivars and Japanese elite cultivar ‘Koshihikari’ was performed to extract regions containing large indels (10–51 bp) shared by diverse cultivars. To design indel markers for the discrimination of genomic regions between ‘Koshihikari’ and other diverse cultivars, we subtracted the indel regions detected in ‘Koshihikari’ from those shared in other cultivars. Two sets of indel markers, KNJ8-indel (shared in eight or more cultivars, including ‘Khao Nam Jen’ as a representative tropical japonica cultivar) and C5-indel (shared in five to eight cultivars), were established, with 915 and 9,899 indel regions, respectively. Validation of the two marker sets by using 23 diverse cultivars showed a high PCR success rate (≥95%) for 83.3% of the KNJ8-indel markers and 73.9% of the C5-indel markers. The marker sets will therefore be useful for the effective breeding of Japanese rice cultivars.  相似文献   

19.
With the practice of conservation agriculture (CA) soil water and nutrient dynamics are modified by the presence of a mulch of crop residues and by reduced or no-tillage. These alterations may have impacts on crop yields. The crop growth model DSSAT (Decision Support Systems for Agrotechnology Transfer) has recently been modified and used to simulate these impacts on crop growth and yield. In this study, we applied DSSAT to a long-term experiment with maize (Zea mays L.) grown under contrasting tillage and residue management practices in Monze, Southern Province of Zambia. The aim was (1) to assess the capability of DSSAT in simulating crop responses to mulching and no-tillage, and (2) to understand the sensitivity of DSSAT model output to input parameters, with special attention to the determinants of the model response to the practice of CA. The model was first parameterized and calibrated for the tillage treatment (CP) of the experiment, and then run for the CA treatment by removing tillage and applying a mulch of crop residues in the model. In order to reproduce observed maize yields under the CP versus CA treatment, optimal root development in the model was restricted to the upper 22 cm soil layer in the CP treatment, while roots could optimally develop to 100 cm depth under CA. The normalized RMSE values between observed and simulated maize phenology and total above ground biomass and grain yield indicated that the CA treatment was equally well simulated as the CP treatment, for which the model was calibrated. A global sensitivity analysis using co-inertia analysis was performed to describe the DSSAT model response to 32 model input parameters and crop management factors. Phenological cultivar parameters were the most influential model parameters. This analysis also demonstrated that in DSSAT mulching primarily affects the surface soil organic carbon content and secondly the total soil moisture content, since it is negatively correlated with simulated soil water evaporation and run-off. The correlations between the input parameters or crop management factors and the output variables were stable over a wide range of seasonal rainfall conditions. A local sensitivity analysis of simulated maize yield to three key parameters for the simulation of the CA practice revealed that DSSAT responds to mulching particularly when rooting depth is restricted, i.e., when water is a critical limiting crop growth factor. The results of this study demonstrate that DSSAT can be used to simulate crop responses to CA, in particular through simulated mulching effects on the soil water balance, but other, often site-specific, factors that are not modeled by DSSAT, such as plough pan formation under CP or improved soil structure under CA, may need to be considered in the model parameterization to reproduce the observed crop yield effects of CA versus CP.  相似文献   

20.
This study analysed the alleviating effect of elevated CO2 on stress‐induced decreases in photosynthesis and changes in carbohydrate metabolism in two wheat cultivars (Triticum aestivum L.) of different origin. The plants were grown in ambient (400 μl l?1) and elevated (800 μl l?1) CO2 with a day/night temperature of 15/10 °C. At the growth stages of tillering, booting and anthesis, the plants were subjected to heat stress of 40 °C for three continuous days. Photosynthetic parameters, maximum quantum efficiency of photosystem II (PSII) photochemistry (Fv/Fm) and contents of pigments and carbohydrates in leaves were analysed before and during the stress treatments as well as after 1 day of recovery. Heat stress reduced PN and Fv/Fm in both wheat cultivars, but plants grown in elevated CO2 maintained higher PN and Fv/Fm in comparison with plants grown in ambient CO2. Heat stress reduced leaf chlorophyll contents and increased leaf sucrose contents in both cultivars grown at ambient and elevated CO2. The content of hexoses in the leaves increased mainly in the tolerant cultivar in response to the combination of elevated CO2 and heat stress. The results show that heat stress tolerance in wheat is related to cultivar origin, the phenological stage of the plants and can be alleviated by elevated CO2. This confirms the complex interrelation between environmental factors and genotypic traits that influence crop performance under various climatic stresses.  相似文献   

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