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1.
为了调查内蒙古呼和浩特地区犬猫弓形虫病的流行情况,采用酶联免疫吸附试验和胶体金免疫层析法对呼和浩特地区共254只犬猫进行弓形虫抗体检测,采用胶体金免疫层析法对阳性感染犬猫进行抗原检测.结果显示,利用酶联免疫吸附试验检测犬的弓形虫阳性率为12.6%,猫为10.3%;利用胶体金免疫层析方法检测犬的弓形虫阳性率为11.4%,猫为9.2%;利用胶体金免疫层析方法对阳性犬猫进行抗原检测,检出率分别为23.8%和33.3%.调查表明,不同来源犬猫的弓形虫感染率差异显著,因此,加强对散养及流浪犬猫的管理是非常必要的.同时建议加强血清学监测,以便及时有效地采取预防措施. 相似文献
2.
为了研究呼和浩特地区犬弓形虫感染情况,试验采用酶联免疫吸附试验(ELISA)对不同来源、不同性别、不同年龄试验动物的弓形虫抗体IgG与IgM进行检测.结果表明:酶联免疫吸附试验检测犬弓形虫抗体IgG与IgM 性率分别为12.4%和4.6%;不同来源犬的弓形虫感染率有显著差异,不同性别犬感染率无明显差异,随着年龄的增长I... 相似文献
3.
采用酶联免疫吸附试验（ELISA）检测194份犬猫血样,测得弓形虫IgG抗体阳性血清36份,阳性率为18．56％。不同来源的犬猫血清弓形虫IgG抗体阳性率不同,流浪犬猫的平均阳性率为27．3％,其中流浪犬阳性率最高为30．77％,其次流浪猫阳性率为25％,农村犬阳性率为22．86％,养犬场犬阳性率为15％,宠物犬阳性率为12．5％。不同来源的犬猫之间感染率差异不显著（P〉0.05）,宠物猫样本数少,未检测出阳性血清。胶体金免疫层析法和酶联免疫吸附试验对比试验显示,这两种方法对猫的弓形虫抗体检测结果基本相符。 相似文献
4.
为了解兰州地区宠物犬弓形虫病的感染情况以及不同弓形虫检测方法检测结果的差异,本研究对兰州地区272例宠物诊所受检犬只进行弓形虫阳性检出率的统计分析,对66份犬血样进行间接血凝试验(IHA)与酶联免疫吸附试验(ELISA)检测,并将2种方法结果进行比较。结果表明弓形虫的感染率与年龄有关,随着年龄的增长感染率增加,犬只小于1岁感染率最低3.77%,1～3岁感染率为10.59%,3～5岁感染率为15.28%,大于5岁时感染率最高22.58%,不同性别之间感染无显著差异(P>0.05);IHA与ELISA检测结果经t检验比较差异不显著(P>0.05),2种检测方法中,IHA方法适用于大规模流行病学调查。 相似文献
5.
为了解兰州地区宠物犬弓形虫病的感染情况以及不同弓形虫检测方法检测结果的差异,本研究对兰州地区272例宠物诊所受检犬进行弓形虫阳性检出率的统计分析;对66份犬血样进行间接血凝试验（IHA）与酶联免疫吸附试验（ELISA）检测,并将2种方法进行比较。结果表明,弓形虫的感染率与年龄有关,随着年龄的增长感染率增加,犬小于1岁感染率最低,为3.77%,1～3岁感染率为10.59%,3～5岁感染率为15.28%,大于5岁时感染率最高,为22.58%,不同性别之间感染无显著差异（P>0.05）;IHA与ELISA检测结果经t检验比较差异不显著（P＞0.05）。 相似文献
6.
为了解重庆市犬和猫弓形虫感染及流行情况,采集重庆市渝北区猫血清76份,重庆18个区县的犬血清789份,用酶联免疫吸附试验(ELISA)检测血清中的弓形虫抗体.结果表明,76份猫血清中阳性5份,阳性率6.58％;789份犬血清中阳性159份,阳性率20.15％,其中城市宠物犬432份,阳性率12.04％,农村家养犬357... 相似文献
7.
为监测自2002年起弓形虫病防控技术实施以来上海市区犬猫弓形虫病的感染情况,本研究应用弓形虫酶联免疫吸附试验(ELISA),对2014年本市4个城区免疫点收治的共291条犬和91只猫的血清样品进行了抗体检测。ELISA检测结果显示,2014年犬、猫弓形虫抗体阳性率分别为5.50%和5.49%,不同季节间差异显著(P0.05),春季最高,为16.48%。本次调查表明上海市区仍然存在犬猫弓形虫感染,但弓形虫病防控技术的实施对降低其感染起到一定作用。笔者对16例弓形虫抗体阳性家养犬病例和5例弓形虫抗体阳性家养猫病例进行了回访调查,结果发现,该21例动物主人的弓形虫检测结果均为阴性。 相似文献
8.
为研究温州市犬弓形虫病感染情况,应用酶联免疫吸附试验,检测351份血液样本。结果显示,本地区犬的弓形虫Ig G抗体阳性率为13.39%(47/351);公犬与雌犬阳性率差异不显著(P0.05),绝育/去势犬的阳性率显著低于未进行绝育/去势的犬(P0.05);成年犬Ig G抗体阳性率大大高于幼年犬和老年犬;流浪犬抗体阳性率最高,训导场内的犬最低,家养犬居中;不同饲喂食物下的犬,其Ig G抗体阳性率差异较大。 相似文献
9.
应用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)对2014-2015年间采集于西藏部分地区的454份藏猪血清进行了弓形虫血清学检测。结果显示,2015年检测出阳性血清116份,阳性率为25.6%。统计数据表明,西藏地区藏猪猪群中存在弓形虫感染。该研究旨在掌握西藏地区藏猪群中弓形虫感染的情况,为制定该病的预防及防治提供参考依据。 相似文献
10.
目的：采集龙岩市部分地区牛、羊血清,检测福建省牛和羊弓形虫病的感染情况。方法：应用酶联免疫吸附试验检测牛和羊血清弓形虫IgG抗体。结果：49份牛血清弓形虫IgG抗体均呈阴性;35份羊血清中,8份弓形虫IgG抗体呈阳性,阳性率为22．86％（8／35）。结论：龙岩市部分地区羊弓形虫的感染率较高,应引起人们重视。 相似文献
11.
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%). Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada. 相似文献
12.
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
13.
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection. 相似文献
15.
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
16.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated. Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively. 相似文献
17.
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
18.
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%). Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada. 相似文献
20.
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario. Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously. 相似文献
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