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1.
在根系分隔盆栽条件下,采用15N土壤稀释标记方法,研究了“小麦/玉米/大豆”三熟套作体系不同作物间的相互作用及氮素的转移、吸收利用特性。结果表明,“小麦/玉米/大豆”套作体系促进小麦对肥料氮和土壤氮的吸收,不分隔处理的生物产量、15N总吸收量和总回收率得到显著提高,土壤残留15N丰度及总氮含量明显降低;玉米表现出套作优势（Awc<0,NCRwc<0）,不分隔处理的籽粒产量、籽粒15N吸收量、15N总回收率、土壤残留15N丰度及总氮含量较分隔处理提高17.17%、24.52%、17.63%、13.9%和10.1%;大豆表现出套作劣势,不分隔处理的15N总吸收量、籽粒15N吸收量、15N总回收率和土壤残留15N丰度降低,土壤总氮含量提高6.06%。“小麦/玉米/大豆”套作体系存在氮素的双向转移,以玉米向小麦、大豆向玉米转移为主。  相似文献   

2.
以两年生红富士/平邑甜茶苹果为试材,采用15N同位素示踪技术,研究牛粪与生物炭不同配比对苹果根系生长、土壤特性和氮素吸收利用的影响,为苹果生产中合理施肥及可持续发展提供依据。试验共设6个处理:对照(CK)、100%牛粪(T1)、75%牛粪+25%生物炭(T2)、50%牛粪+50%生物炭(T3)、25%牛粪+75%生物炭(T4)和100%生物炭(T5)。结果表明,牛粪和生物炭混施可显著改善土壤理化性状,增加土壤有机质、碱解氮、速效磷和速效钾含量,降低土壤容重。苹果根际土壤中的微生物均以细菌占绝对优势,放线菌次之,真菌含量最少;添加牛粪和生物炭均显著提高了根际土壤的细菌、放线菌和真菌数量,其中T2处理细菌、放线菌和真菌数量均达到最高。牛粪和生物炭混施还可促进苹果根系生长,根尖数、根表面积和根系活力等指标均在T2处理达到最高,分别较对照提高47.90%、33.47%、44.67%。表明T2处理可显著促进苹果根系的生长和根系活力的提高。与对照相比,牛粪和生物炭混合处理显著提高了苹果各器官的Ndff值、全氮量和15N吸收量,提高了15N利用率和15N残留率,降低了15N损失率。各处理15N利用率和15N残留率趋势为牛粪和生物炭混合处理最高,单施牛粪或生物炭处理次之,对照最低; 15N损失率呈相反的变化趋势。其中以T2处理效果最好,15N利用率和15N残留率最高, 15N损失率最低, 15N利用率较对照提高5.51%, 15N损失率较对照降低14.52%。综合分析认为, 75%牛粪+25%生物炭处理(T2)对苹果根系生长、土壤特性和氮素吸收利用的效果最好。  相似文献   

3.
采用盆栽试验,研究施肥方式(点施于距土表1、5 cm土柱中心位置,分别记为M1、M2;距土表0~5、0~10 cm土壤与氮肥混匀施用,分别记为M3、M4)和氮肥用量(N 0、0.3、0.6、0.9、1.2 g/盆)对直播稻苗期生物量、根系形态及氮素吸收的影响,以期为直播稻科学施用氮肥提供理论依据。结果表明,M1、M2施肥方式下N0.6处理地上部和根系生物量都显著高于其他处理,增幅分别为17.8%~84.8%和13.9%~46.9%,根系形态指标表现为N0.3和N0.6高于N1.2处理;M3、M4施肥方式下N1.2处理地上部和根系生物量都显著高于其他处理,增幅分别为32.6%~36.3%和14.9%~16.2%,根系形态指标有随着施氮量增加而增加的趋势。施肥方式和氮肥用量对氮素吸收量的影响趋势与生物量表现一致,氮素吸收利用率表现为点施条件下M1M2,混施条件下M3M4。相关分析结果表明,水稻地上部生物量及其氮素吸收与根系形态参数及氮素吸收量存在显著正相关关系。综上所述,低氮条件下浅层点施、高氮条件下浅层混施有利于促进水稻苗期的生长发育,稳固良好的根系形态特性,增强其对养分的吸收利用。  相似文献   

4.
以15年生嘎啦苹果/平邑甜茶为试材,采用C、N双标记技术,研究果实发育期不同施氮方式(传统一次性施氮、分次施氮和渗灌施氮,分别用ON、TN和IN表示)对苹果植株碳氮营养吸收、利用、分配、损失及果实产量和品质的影响。结果表明:至果实成熟期,苹果各器官Ndff值均为INTNON,新生器官间(果实、叶片和1年生枝)差异显著。植株全氮量和~(15)N吸收量均以IN处理最大,ON处理最低。与ON处理相比,TN和IN处理~(15)N利用率分别提高了41.63%和68.60%,而~(15)N损失率分别降低了10.60%和18.63%。各处理不同土层~(15)N残留量差异显著,0—40 cm土层~(15)N残留量为INTNON,60—120 cm土层趋势相反。TN和IN处理果实和贮藏器官(多年生枝、中心干和粗根)的~(13)C分配率均显著高于ON处理,而营养器官(叶片和1年生枝)的~(13)C分配率则以ON处理最高,IN处理最低。同时,在IN处理下,苹果产量、硬度、可溶性糖和糖酸比等品质指标均达到最高水平。综上,渗灌施氮通过降低氮素损失,显著促进植株对氮素的吸收利用,并优化光合产物在各器官间分配,从而最有利于苹果产量和品质的提高。  相似文献   

5.
果园生草对~(15)N利用及土壤累积的影响   总被引：1，自引：0，他引：1  
彭玲  文昭  安欣  姜翰  姜远茂 《土壤学报》2015,52(4):950-956
以2 a生红富士/平邑甜茶为试材,采用15N同位素示踪技术,研究了种植3种牧草(白三叶、鼠茅草和黑麦草)对苹果植株生长,氮素利用、损失及其在0~60 cm土层残留情况的影响。结果表明:与单作苹果相比,生草栽培后苹果植株总鲜重、新梢粗度、新梢长度及根冠比皆成增加的趋势;苹果植株根长、根长密度及根表面积均以种植白三叶处理最大,其次为种植鼠茅草,最低的为单作苹果;不同处理间氮素利用率差异显著,种植白三叶后苹果植株氮素利用率最高,种植鼠茅草次之,单作苹果最低;生草栽培后15N残留主要集中在0~20 cm土层,且其15N残留量显著高于单作苹果。而在20~40 cm及40~60 cm土层15N残留量则以单作苹果最高,种植黑麦草次之,最低的为种植白三叶;生草栽培后氮素损失率为单作苹果种植黑麦草种植鼠茅草种植白三叶。表明种植白三叶、鼠茅草及黑麦草在促进苹果植株氮素利用的同时,也一定程度上减弱了土壤氮素的损失。  相似文献   

6.
为探究有机肥腐熟度对配施化肥氮利用率的作用机制,利用~(15)N标记技术进行意大利生菜盆栽试验,从堆肥过程中选取不同腐熟度的有机肥[按照种子发芽指数(GI值)为50%、80%和100%进行堆肥的腐熟度区分],研究施~(15)NPK化肥(对照, CK)、~(15)NPK+GI 50%有机肥(GI50)、~(15)NPK+GI 80%有机肥(GI80)、~(15)NPK+GI 100%有机肥(GI100) 4个处理对意大利生菜化肥氮的转化、吸收和利用的影响。结果表明,与CK处理相比,添加有机肥处理意大利生菜生物量、~(15)N吸收量与~(15)N利用率分别显著提高30.5%～56.1%、 40.0%～91.0%和15.5%～41.8%(P0.05), GI80处理较GI50处理生物量、~(15)N吸收量与利用率分别显著提高17.1%、31.8%和35.4%(P0.05), GI100处理较GI50处理生物量、~(15)N吸收量与利用率分别显著提高19.6%、15.8%和22.8%(P0.05)。试验期间,添加有机肥处理较CK处理土壤~(15)NH_4~+-N显著提高44.9%～74.2%(P0.05), ~(15)NO_3~--N显著降低8.4%～38.1%(P0.05),净硝化率显著降低10.8%～24.6%(P0.05);GI80处理较GI50处理土壤~(15)NH_4~+-N提高7.9%～11.5%, ~(15)NO_3~--N显著降低18.5%～50.4%(P0.05),净硝化率显著降低15.0%～28.2%(P0.05);GI100处理较GI50处理土壤~(15)NH_4~+-N显著提高11.5%～26.9%(P0.05), ~(15)NO_3~--N显著降低15.8%～22.7%(P0.05),净硝化率显著降低12.5%～23.9%(P0.05)。土壤微生物量氮(MB~(15)N)缓慢上升,添加有机肥处理较CK处理显著提高67.3%～94.1%(P0.05),GI80处理较GI50处理提高6.0%～23.8%,GI100处理较GI50处理显著提高6.9%～25.5%(P0.05)。各处理MB~(15)N占MBN的54.9%～71.6%(P0.05)。相关分析结果表明, MB~(15)N、~(15)NH_4~+-N与~(15)N吸收量、~(15)N利用率呈现极显著正相关关系,且RDA分析结果说明MB~(15)N是影响化肥~(15)N吸收利用的关键驱动因子。因此,有机无机配施体系中适当增加有机肥的腐熟度(GI≥80%)能够明显增强土壤微生物的固氮能力,提高土壤氮素水平,减缓土壤铵态氮向硝态氮的转化速度,降低土壤净硝化速率,从而提高化肥氮的利用效率。  相似文献   

7.
以"M9T337"苹果幼苗为试材,利用~(15)N同位素示踪技术,研究了等氮量投入下,不同有机物料单施及混施对苹果幼苗生长、~(15)N吸收利用及土壤特性的影响。试验设置CK(只施用化学肥料,不施有机物料)、S(秸秆)、B(生物炭)、F(牛粪)、SB(1/2秸秆+1/2生物炭)、SF(1/2秸秆+1/2牛粪)、FB(1/2牛粪+1/2生物炭)、SFB(1/3秸秆+1/3牛粪+1/3生物炭)8个处理。结果表明:施用有机物料可以促进苹果幼苗的生长,其中SFB处理植株鲜重、株高、茎粗、叶面积、根系活力达到最优,显著高于CK和单施有机物料的处理。添加有机物料能降低土壤容重、增加孔隙度、提高土壤含水量,其中施用生物炭的处理土壤容重降幅较大、孔隙度较高。处理期间,有机物料混合施用的处理土壤矿化氮含量、土壤酶活性及微生物数量均优于有机物料单独施用的处理。与CK和单施有机物料的处理相比,有机物料混合施用显著提高了苹果幼苗~(15)N利用率和土壤~(15)N残留率,降低了~(15)N损失率,其中3种有机物料混施效果最好。综合分析可知,有机物料能促进苹果幼苗生长,改良土壤性质,促进植株对~(15)N的吸收利用,其中牛粪、秸秆和生物炭混合施用的处理(SFB处理)效果最佳。研究结果以期为有机物料在苹果园土壤质量提升和化肥减施增效中的应用提供依据。  相似文献   

8.
土壤C/N对苹果植株生长及氮素利用的影响   总被引：4，自引：0，他引：4  
土壤C/N是土壤氮素循环的重要影响因素。本研究以2年生"富士"/平邑甜茶为试验材料, 应用15N示踪技术研究了不同土壤C/N[6.21(CK)、10、15、20、25、30、35和40]对苹果植株生长及氮素利用和损失的影响。结果表明: 随着土壤C/N比值的逐渐增大, 苹果新梢长度和植株鲜重均呈先升高后降低的变化趋势, C/N=15、20和25的3个处理苹果新梢长度和植株鲜重最大, 三者间无显著差异, 但均显著高于其他处理。不同C/N处理间植株15N利用率存在差异, 土壤C/N=25时, 植株15N利用率最大, 为22.87%, 与C/N=20的处理间无显著差异, 但两者均显著高于其他处理; 土壤C/N=40时, 植株15N利用率最低, 仅为15.43%, 低于CK处理的16.65%。土壤C/N处于15~25时, 植株吸收的氮素来自于肥料氮的比例较高; 而土壤C/N较低(<15)或太高(>25)时, 植株吸收的氮素来自于土壤氮的比例较高。土壤氮素残留量随土壤C/N的增大逐渐增加, C/N=40处理的土壤氮素残留量是CK的1.32倍。随着土壤C/N比值的逐渐增大, 肥料氮损失量呈先减少后增加的变化趋势, 以C/N=25时最少, 仅为施氮量的49.87%, 而对照最大, 为61.54%。因此, 综合土壤C/N对苹果植株生长及氮素平衡状况来看, 土壤C/N为15~25时, 能促进植株的生长发育, 降低氮肥损失, 提高肥料利用率。  相似文献   

9.
地下部分隔对间作小麦养分吸收和白粉病发生的影响   总被引：8，自引：1，他引：8  
通过根系分隔的盆栽试验,探讨了地下部分隔对小麦//蚕豆间作系统中小麦的养分吸收和白粉病发生的影响。结果表明:根系不分隔小麦的生物量和N、P、K吸收量均大于尼龙网分隔处理和根系完全分隔处理。根系分隔方式还影响了小麦白粉病的发生,小麦白粉病的发病率和发病指数均表现为根系不分隔(I)尼龙网分隔(M)完全分隔(P)。表明地下部的相互作用改善了小麦的生长,提高了小麦抗白粉病的能力。  相似文献   

10.
应用~(15)N示踪法研究咸水滴灌棉田氮肥去向   总被引：4，自引：0，他引：4  
通过田间小区试验和微区试验相结合研究滴灌条件下不同灌溉水盐度、灌水量和施氮量对棉田土壤中氮肥去向的影响。试验设置3种灌溉水盐度(电导率,EC):0.35、4.61和8.04 d S m-1(分别以S0.35、S4.61和S8.04表示);2个灌水量:405和540 mm;同时设置2个施氮水平:240、360 kg hm-2(360 kg hm-2为当地棉田推荐氮肥用量)。结果表明:S0.35和S4.61灌溉处理的棉花氮素吸收量和产量无显著差异,分别较S8.04灌溉处理高出27.46%和33.65%、21.29%和21.63%。灌溉水盐度主要通过影响棉花单株结铃数来影响棉花产量。增加施氮量和灌水量,棉花氮素吸收量和产量均有所增加。15N同位素标记试验结果表明:植物15N回收率在34.20%~62.51%之间,随灌溉水盐度的增加,植物15N回收率呈现先增加后减小的趋势,S0.35、S4.61处理较S8.04处理分别高出30.70%和41.77%;增加灌水量和施氮量可显著提高植物15N回收率。土壤15N残留率随灌溉水盐度的增加而增加,S4.61和S8.04处理的土壤15N残留率较S0.35处理分别高出3.48%和23.22%。施氮量由240 kg hm-2增加至360 kg hm-2,土壤15N残留率增加9.51%。各处理15N淋洗损失率在0.35%~3.59%之间,低施氮量下,S0.35和S4.61处理的15N淋洗损失率无显著差异,S8.04处理的15N淋洗损失率分别较S0.35、S4.61处理高出1.87倍和0.84倍;高施氮量下,15N淋洗损失率随灌溉水盐度的增加而显著增加。增加灌水量和施氮量,15N淋洗损失率均显著增加。  相似文献   

11.
为探求适于晋西黄土区果农间作系统的水分调控措施,选取该地区典型的苹果×大豆间作系统为研究对象,结合覆盖与调亏灌溉2种节水措施,分析了不同水分调控措施对苹果和大豆根系空间分布、耗水量与水分利用等指标的影响。试验设置灌溉上限3个水平:田间持水量的55%(W1,低水),70%(W2,中水)和85%(W3,高水),2种覆盖材料:秸秆覆盖(M1)和地膜覆盖(M2)。结果表明:水分调控措施增加了苹果和大豆总根长密度,且扩大了苹果在水平和垂直方向上的根长分布。苹果根长密度与距树行距离呈负相关,而大豆则呈正相关,且均与垂直深度存在负相关关系。大豆鼓粒期土壤水分随距树行距离的增加先减后增,最小值为距树行1.5～2.0m,与清耕(CK0)和单一覆盖(CK1和CK2)相比,水分调控措施能显著提高0—60cm土层内的土壤水分。聚类分析表明水分调控下苹果和大豆主要水分竞争区域为距树行0.5～1.5m、垂直方向0—40cm土层范围内。M2W2处理苹果细根集中分布在20—40cm土层,大豆细根主要在0—20cm土层,根系错位分布缓解了种间水分竞争,其耗水量可较W3组减少40～50mm,且其产量和水分利用可分别较其他水分调控措施提高29.37%～41.92%,12.29%～53.35%,同时可使间作系统净收益最大,可达2 976.5元/hm~2。由此建议在未坐果的幼龄苹果树行间间作大豆时采用地膜覆盖措施,同时在分枝期灌水150m~3/hm~2,结荚期灌水400m~3/hm~2,鼓粒期灌水300m~3/hm~2,可显著提高间作系统水分利用水平和经济效益。  相似文献   

12.
为了研究晋西黄土区果农间作系统中土壤养分分布特征,选取研究区具有典型代表性的4,6和8年生苹果(Malus pumila)+大豆(Glycine max)间作系统为研究对象,对间作系统土壤养分(速效钾、速效磷、有机质、全氮)含量进行了定位、定量监测。结果表明:(1)在垂直方向上,不同树龄苹果+大豆间作系统速效钾、速效磷和有机质含量均随着土层深度增加而减少,而全氮含量表现为先增加再减少;(2)在水平方向上,随着距树行距离的增加,4年生间作系统与6年生间作系统土壤养分总体上呈现"W"型分布,而在8年生间作系统中呈现"M"型分布(全氮除外),极值分别出现在距树行1.5,2.5,3.5m处,且极大值点养分含量与极小值点含量有显著差异(p0.05);(3)随着树龄增长,果树与农作物的养分竞争关系逐渐发生变化,且在8年生间作系统中养分竞争最激烈。基于间作系统土壤养分特征,建议在田间施肥管理时,应以施氮肥为主,且随着树龄的增长应该适时增大复合界面的距离或停止复合经营。  相似文献   

13.
Appropriate 15N-labeling methods are crucial for estimating N2-fixation in trees used in agroforestry systems. A 4-year field experiment was conducted on an Alfisol in Southwestern Nigeria to compare the estimates of N2 fixed in Leucaena leucocephala, using two non-N2-fixing leguminous trees, Senna siamea and S. spectabilis, as reference plants and three different methods of introducing 15N into soil. The atom % 15N uptake pattern (as reflected in the leaves) was identical in both N2- and non-N2-fixing tree species irrespective of the 15N-application method. There was a significant decline in atom % 15N excess in the leaves of L. leucocephala (from 0.266 to 0.039), S. siamea (0.625 to 0.121), and S. spectabilis (from 0.683 to 0.118) from the first sampling 12 months after planting and the second sampling 18 months after sampling. From the second harvest in 1991 until the end of the experiment (fifth) harvest in 1993, however, the atom 15N % excess decline in leaves of the three species was less pronounced and depended on the method of 15N application. In those plants to which the tracer was applied once at planting, the 15N decline was steady between the second and the last prunings. In the split-application treatment, the atom 15N % excess increased slightly at the third pruning and decreased during the subsequent two prunings. The reference tree and the method of 15N application influenced the estimated proportion of N derived from atmospheric N2 by L. leucocephala, calculated as 73 and 64%, corresponding to 119 and 98 kg N ha-1 of N2 fixed per 6 months, when S. spectabilis and S. siamea were used as reference trees, respectively. The approach by which 15N-labeled fertilizer was applied to the soil in three splits gave slightly higher estimates of N derived from the atmosphere but this was of little agronomic significance because total N2 fixed was similar for all methods.  相似文献   

14.
为探究适于晋西黄土区果农间作系统滴灌水肥一体化管理制度,以典型的苹果-大豆间作系统为研究对象,设置灌水和施肥两因素,分析不同水肥调控措施对土壤含水量分布、苹果和大豆光合生理特征、大豆生长和产量以及间作系统水分利用等指标的影响。试验在大豆4个关键需水期进行灌水,肥料随灌溉水施入,每次设置不同灌水上限和施肥水平,4个灌水量上限水平分别为:田间持水量(Fc)的60%(W1),70%(W2),80%(W3)和90%(W4),3个施氮水平:纯N 59.40 kg/hm^2(F1),92.00 kg/hm^2(F2),124.32 kg/hm^2(F3),对照处理(CK)整个生育期不灌水不施肥,仅在播种前施入基肥。结果表明:各水肥处理土壤含水量在水平和垂直方向上具有显著差异,灌水量对土壤含水量的影响程度高于施肥量和水肥交互作用。苹果和大豆的净光合速率(Pn)和蒸腾速率(Tr)的日变化特征相似,均为单峰型曲线,最大值均为W3F2处理。各处理大豆株高、茎粗和叶面积指数(LAI)分别较对照组提高了1.3%~32.3%,2.8%~33.9%和3.4%~125.9%,其中最大值均出现在W3F2处理,该处理大豆产量和间作系统水分利用效率(WUE)也最优,较其他处理分别提高了10.9%~99.3%和8.0%~70.0%。在播种至出苗期、幼苗期至分枝期、开花结荚期和鼓粒期可以设置80%Fc的灌水上限,同时在大豆幼苗期至分枝期、结荚期和鼓粒期分别施加92.00 kg/hm^2的氮肥,该水肥管理方式使苹果—大豆间作系统获得较高的作物产量及水分利用效率,可为该地区间作系统滴灌水肥一体化管理提供参考。  相似文献   

15.
A field experiment on dhaincha, sunflower, and sorghum plants grown in monocropping and intercropping systems was conducted to evaluate growth and nitrogen (N2) fixation using 13carbon (C) and 15N natural abundance techniques. Intercropping of sesbania/sorghum showed a greater efficiency than monocropping in producing dry matter during the entire growth period, whereas the efficiency of producing dry matter in the sesbania/sunflower intercropping was similar to that in the monocropping system. Moreover, sorghum plants (C4) were more competitive than sesbania (C3) for soil N uptake, whereas sesbania seemed to be more competitive than its associated sunflower (C3). Nitrogen uptake in the mixed stand of sesbania/sorghum was improved as a result of the increase in soil N uptake by the component sorghum and the greater root nodule activity of component sesbania without affecting the amount of N2 fixed. The Δ 13C in plant materials was affected by plant species and the cropping system.  相似文献   

16.
Total nitrogen (N) concentration (N%), N derived from 15N-fertilizer (Ndff%), amount of 15N uptake (ANU) in main organs (leaves, shoots, roots, fruits), and N use efficiency (NUE) were measured to assess N absorption, distribution, and utilization of Red Fuji apple trees across two years using a 15N-enriched urea method. The N% in leaves and fruits decreased while those in shoots and roots increased in both years. The Ndff% and ANU in roots were highest at fruitlet stage than those in leaves, shoots and fruits at mature stages. This suggested that the absorbed 15N by roots was redistributed to new organs. The N% was lower while Ndff% and ANU were greater in 2008 than 2007. The most 15N absorbed was accumulated in the trunks, main and coarse roots and smaller in the fine roots and biennial branches. The NUE in 2007 and 2008 reached 9.9% and 12.2% respectively.  相似文献   

17.
Abstract

The contribution of biological nitrogen fixation (BNF) to the N nutrition of six annual forage legumes, subterranean clover (Trifolium subterraneum), burr medic (Medicago polymorpha), balansa clover (Trifolium michelianum), Persian clover (Trifolium resupinatum), yellow serradela (Ornithopus compressus), and pink serradela (Ornithopus sativus) was evaluated by the 15N natural abundance technique, using four grass species (Briza máxima, Bromus mollis, Hordeum berteroanum, Avena barbata) and two composite species (Leontodon leysseri and Hedipnois cretica) as reference plants. An additional objective was to determine whether alternative legume species to those in common use (T. subterraneum and M. polymorpha) in the area, could improve BNF. The field studies were conducted in two edaphic conditions, granitic (Entisol) and clay (Vertisol) soil, located in Cauquenes, VII Region, in the sub-humid Mediterranean zone of Chile. In the granitic soil the percentages of N derived from fixation were high in all species (74 to 94%); yellow serradela cv. Tauro presented the greatest N content in dry matter and N fixation, equivalent to 91 kg N ha?1. In contrast, pink serradela cv. Cádiz and subterranean clover cv. Gosse presented the lowest N fixation. In the clay soil, under periodically waterlogged conditions, balansa clover cv. Paradana and persian clover cv. Prolific had high percentage values of BNF (>95%) and fixed more N (100.2 and 82.5 kg N ha?1, respectively) than burr medic and subterranean clover cv. Gosse. The present study allowed the identification of new germplasm of high capacity of N fixation which is an additional criterion for selecting species for infertile and waterlogged soil conditions in the Mediterranean area of Chile.  相似文献   

18.
Abstract

The popular and widely used 15nitrogen (N)–isotope dilution method for estimating biological N fixation (BNF) of pasture and tree legumes relies largely on the ability to overcome the principal source of error due to the problem of selecting appropriate reference plants. A field experiment was conducted to evaluate the suitability of 12 non‐N2‐fixing plants (i.e., nonlegumes) as reference plants for estimating the BNF of three pasture legumes (white clover, Trifolium repens L.; lucerne, Medicago sativa; and red clover, Trifolium pratense L.) in standard ryegrass–white clover (RWC) and multispecies pastures (MSP) under dry‐land and irrigation systems, over four seasons in Canterbury, New Zealand. The 15N‐isotope dilution method involving field 15N‐microplots was used to estimate BNF. Non‐N2‐fixing plants were used either singly or in combination as reference plants to estimate the BNF of the three legumes. Results obtained showed that, on the whole, 15N‐enrichment values of legumes and nonlegumes varied significantly according to plant species, season, and irrigation. Grasses and herb species showed higher 15N‐enrichment than those of legumes. Highest 15N‐enrichment values of all plants occurred during late summer under dry‐land and irrigation conditions. Based on single or combined non‐N2‐fixing plants as reference plants, the proportion of N derived from the atmosphere (% Ndfa) values were high (50 to 90%) and differed between most reference plants in the MSP pastures, especially chicory (Cichorium intybus), probably because it is different in phenology, rooting depth, and N‐uptake patterns compared to those of legumes. The percent Ndfa values of all plants studied also varied according to plant species, season, and irrigation in the MSP pastures. Estimated daily amounts of BNF varied according to pasture type, time of plant harvest, and irrigation, similar to those shown by percent Ndfa results as expected. Irrigation increased daily BNF more than 10‐fold, probably due to increased dry‐matter yield of pasture under irrigation compared to dry‐land conditions. Seasonal and irrigation effects were more important in affecting estimates of legume BNF than those due to the appropriate matching of N2‐fixing and non‐N2‐fixing reference plants.  相似文献   

19.
A field experiment was conducted to obtain the N balance sheet for sole crops and intercrops of sorghum [Sorghum bicolor (L.) Moench] and pigeonpeas [Cajanus cajan (L.) Millsp.]. Intercropping gave a significant advantage over sole cropping in terms of dry matter production and grain yield, as calculated on the basis of the land equivalent ratio and area-time equivalent ratio. The N fertilizer use efficiency and atmospheric N2 fixation by pigeonpea were estimated using 15N-labeling and natural abundance methods. The N fertilizer use efficiency of sorghum was unaltered by the cropping system, while that of the pigeonpea was greatly reduced by intercropping. Although intercropping increased the fractional contribution of fixed N to the pigeonpeas, no significant difference was observed between the cropping systems in total symbiotically fixed N. There was no evidence of a significant transfer of N from the pigeonpea to the sorghum. This study showed that use of soil N and fertilizer N by pigeonpeas was almost the same as that by sorghum in sole cropping, indicating the potential competence of pigeonpeas to exploit soil N. However, when N was exhausted by a companion crop in intercropping, the pigeonpea crop increased its dependency on atmospheric N2 fixation. We conclude that knowledge of how N from different sources is shared by companion crops is a prerequisite to establishing strategies to increase N use, and consequently land productivity, in intercropping systems.  相似文献   

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