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1.
棉花数量性状遗传与QTL定位研究进展   总被引:4,自引:0,他引:4  
棉花许多重要的性状多为数量性状。现代分子生物技术的发展为植物数量性状基因的定位、分离等研究提供了条件。从数量性状基因座(QTL)作图群体类型及其特点,QTL定位方法,QTL精细定位、克隆、利用等方面进行了综述,并对今后QTL研究进行了展望。  相似文献   

2.
棉花许多重要的性状多为数量性状。现代分子生物技术的发展为植物数量性状基因的定位、分离等研究提供了条件。从数量性状基因座(QTL)作图群体类型及其特点,QTL定位方法,QTL精细定位、克隆、利用等方面进行了综述,并对今后QTL研究进行了展望。  相似文献   

3.
作物产量性状QTL定位的研究现状及应用前景   总被引:5,自引:0,他引:5  
作物的许多农艺性状和经济性状是数量性状。研究作物数量性状遗传对农作物育种具有十分重要的意义。本文综述了数量性状基因座QTL(quantitative trait locus)定位的原理和常用方法及分子标记在水稻、小麦、玉米、棉花、大豆、番茄、大麦和油菜等重要作物产量性状基因定位中的研究现状,并对目前产量性状QTL定位存在的问题和发展前景进行了探讨  相似文献   

4.
植物QTL研究进展   总被引:1,自引:0,他引:1  
摘要:随着分子生物学和基因组学的发展,作物QTL研究方面取得了极大的进展。采用QTL定位方法研究作物数量性状从通常可见表型发展到基因的表达水平,从某一发育阶段的静态QTL定位发展到全生育期的动态QTL定位。并且作物QTL研究也不断深入,近年来已克隆了番茄,水稻和小麦等多种植物重要农艺性状的QTL,并从分子水平上剖析了它们的作用机理。本文就作物QTL分析的现状,发展趋势以及作物QTL图位克隆中精细定位策略等方面进行了介绍和分析。  相似文献   

5.
陆地棉中G6主要性状主效和上位性QTL分析   总被引:2,自引:0,他引:2  
利用基于混合线性模犁的复合区间作图法对重组近交系(recombinant inbreed line,RIL)"中G6"进行QTL定位,生育期性状共定位了主效QTL位点5个,上位性QTL位点4对,纤维品质性状定位了主效QTL位点1个,上位性QTL位点6对,产量性状定位了主效QTL位点3个,上位性QTL位点5对.其中定位的果枝始节、吐絮期、上半部平均长度、衣分的主效QTL位点均距离最近标记1 cM以下,这有利于在育种实践中主效QTL跟踪检测.定位的霜前花率主效QTL位点具有较高的加性效应和遗传贡献率,应进行QTL精细定位、图位克隆,将会对早熟性育种工作有一定的推动意义.定位的马克隆值、衣分和予指总的遗传贡献率均在30%以上,对性状特征均影响显著.对主效及上位性QTL位点进行遗传效应分析,验证了前人有关数量性状遗传符合主基因与多基因混合遗传的论断,认为此模型是研究数量性状遗传的有效途经;对主效及上位性QTL位点进行A、D亚基因组定位,并对主效及上位性QTL位点在A、D亚基因组上的分布及互作方式进行了详细的分析.全文认为上位性QTL位点和主效QTL位点一样在物种遗传变异和聚合育种中起着重要作用.  相似文献   

6.
基于作物QTL的分子育种研究进展   总被引:6,自引:0,他引:6  
分子标记技术和QTL(Quantitative Traits Loci)定位技术的迅速发展,使得以DNA多态性为基础的分子育种技术的研究不断深入,并在作物遗传育种中得到了一些成功的运用,为解决有关复杂性状的选择问题带来了希望。本文综述了近年来基于作物QTL的分子标记辅助选择及目标性状QTL克隆在作物的产量、品质、抗旱性等数量性状遗传育种中的主要应用,证实了分子育种的有效性;对目前影响分子育种效率的因素及存在的问题、应用前景进行了探讨。  相似文献   

7.
《种业导刊》2012,(5):36-36
由扬州大学农学院徐辰武教授主持的《作物复杂性状遗传分析与基因/QTL定位新方法研究》项目,在国家973计划、863计划等课题资助下,通过多方面的系统研究,形成了一套对复杂性状进行遗传分析和基因/QTL定位的新方法,奠定了我国在国际统计基因组学研究中的重要学术地位。  相似文献   

8.
大豆的许多重要农艺性状和经济性状是受多基因控制的数量性状。针对大豆产量性状、种子品质性状和重要病害的抗性等,综述了近年来大豆数量性状基因座位(quantitative trait locus, QTL)定位研究的进展,并讨论了目前大豆QTL定位研究存在的问题及解决途径。  相似文献   

9.
此文首先简述了数量性状基因(QTL)克隆研究方法,然后较全面的总结了作物QTL克隆的研究进展,最后讨论了QTL调控的分子机理。对37个已成功克隆的QTL进行汇总分析表明:控制作物数量性状的有转录因子、蛋白激酶、细胞分裂素氧化酶类等基因;其中12个已成功克隆的QTL编码转录因子,说明转录调控因子是新的植株形态进化和变异的重要原因。随着测序技术的发展,海量的基因组数据将为QTL的遗传定位和分子剖析提供更多便利,越来越多的QTL将会被克隆出来。  相似文献   

10.
水稻许多重要农艺性状都是数量性状,水稻遗传改良的大部分工作就是改良这些数量性状。1980年以后,随着分子标记的出现,水稻高密度遗传连锁图谱的大量绘制,各种作图群体的迅猛构建,功能强大的各种统计学软件的快速开发,全基因组序列测序的完成,特别是多个QTL精细定位和先后克隆,使得我们对数量性状的认识得到了长足的进步,QTL的研究正在走进新时代。  相似文献   

11.
During the past decade, numerous studies have been published on molecular mapping of Fusarium head blight (FHB) resistance in wheat. We summarize the relevant findings from 52 quantitative trait loci (QTL) mapping studies, nine research articles on marker-assisted selection and seven on marker-assisted germplasm evaluation. QTL for FHB resistance were found on all wheat chromosomes except chromosome 7D. Some QTL were found in several independent mapping studies indicating that such QTL are stable and therefore useful in breeding programmes. We summarize and update current knowledge on the genetics of FHB resistance in wheat resulting from QTL mapping investigations and review and suggest FHB breeding strategies based on the available information and DNA markers.  相似文献   

12.
Fungal diseases are a major constraint for wheat production. Effective disease resistance is essential for ensuring a high production quality and yield. One of the most severe fungal diseases of wheat is Septoria tritici blotch (STB), which influences wheat production across the world. In this study, genomewide association mapping was used to identify new chromosomal regions on the wheat genome conferring effective resistance towards STB. A winter wheat population of 164 North European varieties and breeding lines was genotyped with 15K single nucleotide polymorphism (SNP) wheat array. The varieties were evaluated for STB in field trials at three locations in Denmark and across 3 years. The association analysis revealed four quantitative trait loci, on chromosomes 1B, 2A, 5D and 7A, highly associated with STB resistance. By comparing varieties containing several quantitative trait loci (QTL) with varieties containing none of the found QTL, a significant difference was found in the mean disease score. This indicates that an effective resistance can be obtained by pyramiding several QTL.  相似文献   

13.
Spike length (SL) is one of the most important components of spike morphology, and the uppermost internode represents an ideal organ to study the transportation system. We performed conditional and unconditional quantitative trait locus (QTL) mapping in two unrelated recombinant inbred line populations to precisely detect QTLs for uppermost internode length (UIL) and SL, and to dissect the genetic relationship between these two factors with plant height (PH). Both of the populations were derived from crosses with synthetic wheat. Ten repetitive QTLs for UIL and six environment-independent QTLs for SL were identified in this study, and twelve of these were completely independent of PH. Conditional QTL mapping analysis indicated that SL was more independent to PH than UIL was. The results indicated that the conditional QTL mapping method could evaluate PH component effects on PH, and thus accelerate the selection of suitable loci that improve commercial wheat morphology yet avoid changes to PH.  相似文献   

14.
Much effort has been invested in identifying molecular markers in wheat (Triticum aestivum L.) linked to quantitative trait loci (QTL) that confer resistance to Fusarium head blight (FHB) caused by Fusarium graminearum Schwabe [teleomorph Gibberella zeae (Schwein) Petch]. Even after several generations of crossing and selection by many wheat breeding programs, resistance of the Chinese spring wheat cultivar ‘Sumai 3’ (PI 481542) remains among the most effective. It therefore seems that undocumented resistance QTL present in Sumai 3 were not detected in various mapping studies. Using an extremely susceptible Tibetan landrace (‘Y1193-6’; unknown pedigree) in the creation of a mapping population with Sumai 3, the objective of this research was to identify undocumented resistance QTL in Sumai 3. This was accomplished through collecting disease index (DI) and Fusarium damaged kernel (FDK) phenotypic values along with 305 Diversity Array Technology (DArT) and 52 Simple Sequence Repeat (SSR) marker genotypes on 160 F2:6 recombinant inbred lines (RILs). Disease response evaluations were based on four (two greenhouse and two field) experiments where spray inoculation methods were used. Three QTL were identified on chromosome arms 3BS, 6BL and 2DS explaining 26.1, 10.7 and 18.9% of the phenotypic variation for DI, respectively. The same QTL were also significantly associated with reduced FDK scores and explained 28.0, 11.0 and 23.0% of phenotypic variation. Lines within the mapping population were placed in eight categories with respect to their various QTL combinations. Lines with no QTL were the most susceptible, whereas those with the Sumai 3-derived 3BS and 6BL QTL combined with the 2DS QTL from Y1193-6 were the most resistant. Though the 3BS and 6BL QTL are well-documented, the 2DS resistance QTL, which was contributed by the susceptible parent, confers increased susceptibility when derived from Sumai 3. In this study no new FHB QTL from Sumai 3 was discovered, but results suggest that Sumai 3 contains a QTL for susceptibility on chromosome arm 2DS. Selection against this QTL may potentially increase resistance levels among Sumai 3-derived populations.  相似文献   

15.
W. D. Bovill    W. Ma    K. Ritter    B. C. Y. Collard    M. Davis    G. B. Wildermuth    M. W. Sutherland 《Plant Breeding》2006,125(6):538-543
Crown rot (causal agent Fusarium pseudograminearum) is a fungal disease of major significance to wheat cultivation in Australia. A doubled haploid wheat population was produced from a cross between line ‘W21MMT70’, which displays partial seedling and adult plant (field) resistance to crown rot, and ‘Mendos’, which is moderately susceptible in seedling tests but partially resistant in field trials. Bulked segregant analysis (BSA) based on seedling trial data did not reveal markers for crown rot resistance. A framework map was produced consisting of 128 microsatellite markers, four phenotypic markers, and one sequence tagged site marker. To this map 331 previously screened AFLP markers were then added. Three quantitative trait loci (QTL) were identified with composite interval mapping across all of the three seedling trials conducted. These QTL are located on chromosomes 2B, 2D and 5D. The 2D and 5D QTL are inherited from the line ‘W21MMT70’, whereas the 2B QTL is inherited from ‘Mendos’. These loci are different from those associated with crown rot resistance in other wheat populations that have been examined, and may represent an opportunity for pyramiding QTL to provide more durable resistance to crown rot.  相似文献   

16.
Chromosomal location of genes for carotenoid pigments in Hordeum chilense   总被引:1,自引:0,他引:1  
In wheat, carotenoids are very important for end‐use quality in noodle production. Hexaploid tritordeums are the amphiploids derived from the cross between a wild diploid barley (Hordeum chilense) and durum wheat. Hexaploid tritordeums exhibit a higher carotenoid content than their respective wheat parents. The cross between H1 and H7 lines of H. chilense was used in order to map quantitative trait loci (QTL) for carotenoid content. Multiple interval mapping identified one QTL mapped on chromosome 2. This knowledge is helpful to transfer this favourable trait to other cereal genomes because of the high crossability of H. chilense with other members of the tribe Triticeae.  相似文献   

17.
Rye production in European growing areas is constrained by the soilborne cereal mosaic virus (SBCMV) and the wheat spindle streak mosaic virus (WSSMV). To date, no European rye cultivars are known to exhibit resistance against these viruses. In this study, we pursued a quantitative trait locus (QTL) mapping strategy to identify genomic regions for resistance to SBCMV and WSSMV in rye. Three populations, each comprising 100 lines segregating for resistance to SBCMV and/or WSSMV, were evaluated for disease response at two years in three locations in Germany where soils are naturally infested with SBCMV and WSSMV. In the combined analysis across environments, one QTL for SBCMV resistance on chromosome 5R explained 31.9% of the phenotypic variation in one of the populations. For WSSMV resistance, one QTL explaining up to 64.0% of the phenotypic variation was detected on chromosome 7R in each of the three populations. On the Triticeae homoeologous group 5, we found evidence for synteny of the major QTL for SBCMV resistance between the wheat and rye genomes.  相似文献   

18.
不同盐浓度胁迫下小麦苗期苗高和主根长的QTL分析   总被引:3,自引:0,他引:3  
小麦苗期苗高和主根长是鉴定小麦苗期耐盐性的重要指标。利用小麦品种花培3号×豫麦57获得的DH群体168个株系,在去离子水(对照)以及50,100,200 mmol/L NaCl溶液处理下,进行苗高和主根长的数量性状基因(QTL)定位分析。利用完备区间作图法,共检测到影响苗高和主根长的25个QTL,单个QTL对表型的贡献率为4.19%~23.72%。位于3D染色体区间Xgdm72-Xbarc1119上影响主根长的QTL位点具有最大的遗传效应,贡献率为23.72%;在100 mmol/L和50 mmol/L NaCl处理下,在2D染色体Xwmc170.2-Xgwm539区段,同时检测到影响苗高的2个QTL位点,其贡献率分别为12.59%和8.40%;在100 mmol/L和200 mmol/L NaCl处理下,在4D染色体Xc-fa2173-Xcfe188区段,同时检测到影响主根长的2个QTL位点,其贡献率分别为8.77%和5.70%;在对照和100mmol/L NaCl溶液处理下,在5BL染色体Xgwm213-Xswes861.2区段,同时检测到影响苗高的QTL位点,其贡献率分别为17.49%和6.28%。另外,在50 mmol/L NaCl溶液处理下,4B染色体Xwmc657-Xwmc48区段还定位了1个影响苗高的QTL位点,其贡献率为12.59%;在染色体3A和染色体7D上各检测出与主根长有关的1个不同的QTL;在5A染色体Xbarc358.2-Xgwm186和Xcwem40-Xbarc358.2区间分别检测到1个影响苗高的QTL。这些主效QTL可用于苗高和主根长的分子标记辅助选择。  相似文献   

19.
A wheat (Triticum aestivum L.) recombinant inbred line (RIL) population was used to identify quantitative trait loci (QTL) associated with yield, yield components, and canopy temperature depression (CTD) under field conditions. The RIL population, consisting of 118 lines derived from a cross between the stress tolerant cultivar ‘Halberd’ and heat stress sensitive cultivar ‘Karl92’, was grown under optimal and late sown conditions to impose heat stress. Yield and yield components including biomass, spikes m?2, thousand kernel weight, kernel weight and kernel number per spike, as well as single kernel characteristics were determined. In addition, CTD was measured during both moderate (32–33 °C) and extreme heat stress (36–37 °C) during grain-filling. Yield traits showed moderate to high heritability across environments with a large percentage of the variance explained by genetic effects. Composite interval mapping detected 25 stable QTL for the 15 traits measured, with the amount of phenotypic variation explained by individual QTL ranging from 3.5 to 27.1 %. Two QTL for both yield and CTD were co-localized on chromosomes 3BL and 5DL and were independent of phenological QTL. At both loci, the allele from Halberd was associated with both higher yield and a cooler crop canopy. The QTL on 3BL was also pleiotropic for biomass, spikes m?2, and heat susceptibility index. This region as well as other QTL identified in this study may serve as potential targets for fine mapping and marker assisted selection for improving yield potential and stress adaptation of wheat.  相似文献   

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