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1.
The chemical fertility of the forest soils in the Belgian Ardenne is threatened by acidifying and eutrophying deposition and by the nutrient removal due to timber harvesting. Experiments were launched to evaluate the ability of liming and fertilization to improve foliar nutrition, maintain or restore crown condition and promote tree growth. In 1995, 10 liming and fertilization trials were installed in even-aged stands of European beech (5) and Norway spruce (5) distributed throughout the Ardenne. In each stand, two treatments were tested: liming with 3,000 kg ha−1 of dolomitic limestone and liming plus fertilization with 0 to 800 kg ha−1 of rock phosphate and 0 to 350 kg ha−1 of K2SO4. Between 1995 and 2006, the foliar Ca and Mg status of spruce and beech trees improved in the limed stands, which limited significantly but did not prevent the decline in crown condition triggered by the summer drought in 2003. For spruce, liming also increased the increment in basal area. The additional fertilization increased the foliar nutrition in P but had no significant effects on soil chemistry and tree vitality.  相似文献   

2.
3.
We quantified the effect of water and nutrient availability on aboveground biomass and nitrogen accumulation and partitioning in four species from the southeastern United States, loblolly pine (Pinus taeda), slash pine (Pinus elliottii), sweetgum (Liquidambar styraciflua), and sycamore (Platanus occidentalis). The 6-year-old stands received five levels of resource input (control, irrigation with 3.05 cm water week−1, irrigation + 57 kg N ha−1 year−1, irrigation + 85 kg N ha−1 year−1, and irrigation + 114 kg N ha−1 year−1). Irrigation significantly increased foliage, stem, and branch biomass for sweetgum and sycamore, culminating in 103% and 238% increases in total aboveground biomass. Fertilization significantly increased aboveground components for all species resulting in 49, 58, 281, and 132% increases in total aboveground biomass for loblolly pine, slash pine, sweetgum, and sycamore, respectively. Standing total aboveground biomass of the fertilized treatments reached 79, 59, 48, and 54 Mg ha−1 for loblolly pine, slash pine, sweetgum, and sycamore, respectively. Fertilization increased foliar nitrogen concentration for loblolly pine, sweetgum, and sycamore foliage. Irrigation increased total stand nitrogen content by 6, 14, 93, and 161% for loblolly pine, slash pine, sweetgum, and sycamore, respectively. Fertilization increased total nitrogen content by 62, 53, 172, and 69% with maximum nitrogen contents of 267, 212, 237, and 203 kg ha−1 for loblolly pine, slash pine, sweetgum, and sycamore, respectively. Growth efficiency (stem growth per unit of leaf biomass) and nitrogen use efficiency (stem growth per unit of foliar nitrogen content) increased for the sycamore and sweetgum, but not the loblolly or slash pine.  相似文献   

4.
The effect of ash fertilization on height growth and volume production of Scots pine (Pinus sylvestris L.) was studied on oligotroph peatland in southeast Norway. In the year 1944, plots 15 m × 15 m size were fertilized with 0, 4, 7, and 10 tons ha?1 of wood or peat ash. The area was treeless, but a satisfactory number of pine seedlings were present. All measurements were confined to the central inner plot, 10 m × 10 m area. Most plots were re-fertilized with 10 tons of wood ash ha?1 in the year 1993. Wood ash had higher content of nutrients, and generally, it had greater growth enhancement effect than peat ash. When the amount of ash was increased, volume production significantly increased for the age period 38–50 years and the total production at age 50 years. The mean annual increment during the first 50 years was about 6 m3 ha?1 for the plots applied with 10 tons of ash ha?1. Trees on plots fertilized with 7 or 10 tons in 1944 and replenished with 10 tons ha?1 at age 50 years (1993) had a mean annual increment of 14 m3 ha?1 for the stand age period 51–68 years. Over time some tree roots from control plots and plots fertilized with 4 tons ha?1 have captured nutrients from richer plots. Such effect is to a smaller extent relevant for treatment 7 tons. It is concluded that the content of mineral nutrients of wood and peat ash makes these ashes well suited as fertilizers on peatland.  相似文献   

5.
Green manure of multipurpose trees is known to be a good source of nutrients to crop. However, most agroforestry species do not have adequate phosphorus (P) in their leaves. Supplementing green manure with moderate dose of P is a beneficial strategy to improve food security in Rwanda. This study examines the effects of Calliandra calothyrsus Meissner, Tithonia diversifolia Hensley A.Gray and Tephrosia vogelii Hook.f. green manure applied independently or in combination with triple super phosphate (TSP) and lime on maize yield and P uptake in the Oxic Tropudalf of Rubona, Rwanda. The treatments were the control, lime at 2.5 t ha−1, TSP at 25 and 50 kg P ha−1, leaf of C. calothyrsus, T. diversifolia, and T. vogelii each at 25 and 50 kg P ha−1, respectively. Leaf shrubs biomass, TSP and lime were applied for four consecutive seasons (2001–2004). The results showed that the combination of green manure with TSP at a rate of 50 kg P ha−1 significantly increased maize yield from 24 to 508% when compared to the control and T. divesifolia combined with TSP was leading (508%). Equally, the same treatments as indicated above showed higher P uptake (15.6–18. 6 kg P ha−1) than the control (5 kg P ha−1) and 65% of maize yields variation was explained by total P uptake. The plant residues quality such as C:N ratio, total plant N, and P significantly influenced the variability of maize grain yields.  相似文献   

6.

Elevated nitrogen (N) deposition is changing soil communities around the world and will have unknown consequences for terrestrial ecosystem functions. In this study, we investigated a field experiment that lasted for 13 years to explore the effect of simulated N deposition and seasonal variations on the soil faunal community structure in a temperate natural secondary forest. The experimental design included a control group (0 kg N ha?1 yr?1, CK), low N addition (25 kg N ha?1 yr?1, LN), and high N addition (50 kg N ha?1 yr?1, HN). The results showed that long-term high N addition reduced the soil pH, C/N ratio, and microbial biomass carbon (MBC) and increased the total phosphorus. The soil faunal community structure after high N addition was significantly different from those after the CK and low N addition treatments. The overall trend was that abundance and richness increased under low N addition and decreased under high N addition. Further analysis showed that the abundance of omnivores and detritivores was lowest after high N addition, significantly less than the CK and low N addition. The interaction of N addition and seasonal dynamics had a significant impact on herbivores. We found that these changes were driven by differences in ecological strategies such as food and environmental preferences. Furthermore, temperature, moisture, nutrients, and pH in the soil environment were the key factors driving ecological strategies and environmental factors. Seasonal variations significantly affected the soil faunal community structure, showing the highest abundance, richness, diversity, and functional group abundance and richness of the soil faunal community in September. Nitrogen addition and seasonal dynamics significantly affected the abundance and richness of soil fauna by changing soil nutrient concentrations, MBC, and plant diversity. Our study showed that long-term high N addition reduced the abundance and functional group abundance of the soil fauna in natural secondary forests, while low N addition had a positive effect on soil faunal community structure. Collectively, the results suggest that the seasonal balance of soil fauna is affected after long-term N addition, which increases the seasonal sensitivity of soil fauna.

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7.
Small Appalachian hill farms may benefit economically by expanding grazing lands into some of their under-utilized forested acreages. Our objective was to study the forage production potential of forest to silvopasture conversion. We thinned a white oak dominated mature second growth forested area establishing two orchardgrass-perennial ryegrass-white clover silvopasture replications for comparison with two nearby open pasture replications. After thinning trees, silvopastures were limed, fertilized and seeded. Sheep were fed hay and corn scattered across the area to facilitate removal of residual understory vegetation, disruption of litter layer and incorporation of applied materials into surface soil. Each area was divided into multiple paddocks and rotationally grazed by sheep. Two 1 m2 herbage mass samples were taken from each paddock prior to animal grazing. There was no significant difference in soil moisture between silvopastures and open pastures however, there was adequate rainfall to prevent drought all 3 years. The two silvopastures received 42 and 51% of total daily incident PAR compared to the open field. Total dry forage mass yield from open pasture over the 3 years averaged 11,200 kg ha−1 y−1 and from silvopasture 6,640 kg ha−1 y−1. Silvopastures, however, had a higher PAR use efficiency (PARUE) than open pasture. Hill farms could increase grazing acreages without sacrificing all benefits from trees on the landscape by converting some areas to silvopasture.  相似文献   

8.
The relationships of nitrogen biogeochemistry are reviewed, focusing on forested watersheds in North America, Europe and Japan. Changes in both local and global nitrogen cycles that affect the structure and function of ecosystems are described. Within northeastern United States and Europe, atmospheric deposition thresholds of ~8 and ~10 kg N ha−1 year−1, respectively, result in enhanced mobilization of nitrate. High nitrate concentrations and drainage water loss rates up to 22 kg N ha−1 year−1 have also been found near Tokyo. Although atmospheric deposition may explain a substantial portion of the spatial pattern of nitrate in surface waters, other factors also play major roles in affecting the spatial patterns of nitrogen biogeochemistry. Calcium availability influences the composition of the vegetation and the biogeochemistry of nitrogen. The abundance of sugar maple is directly linked to soil organic matter characteristics and high rates of nitrogen mineralization and nitrification. Seasonal patterns of nitrate concentration and drainage water losses are closely coupled with differences in seasonal temperature and hydrological regimes. Snow-dominated forested catchments have highest nitrate losses during snowmelt. Watersheds in the main island of Japan (Honshu) with high summer temperatures and precipitation inputs have greatest losses of nitrate occur during the late summer. Understanding future changes in nitrate concentrations in surface waters will require an integrated approach that will evaluate concomitantly the influence of both biotic and biotic factors on nitrogen biogeochemistry.  相似文献   

9.
Subtropical evergreen broad-leaved forest is the most widely distributed land-cover type in eastern China. As the rate of land-use change accelerates worldwide, it is becoming increasingly important to quantify ecosystem biomass and carbon (C) and nitrogen (N) pools. Above and below-ground biomass and ecosystem pools of N and C in a subtropical secondary forest were investigated at Laoshan Mountain Natural Reserve, eastern China. Total biomass was 142.9 Mg ha−1 for a young stand (18 years) and 421.9 Mg ha−1 for a premature stand (ca. 60 years); of this, root biomass was from 26.9 (18.8% of the total) to 100.3 Mg ha−1 (23.8%). Total biomass C and N pools were, respectively, 71.4 Mg ha−1 and 641.6 kg ha−1 in the young stand, and 217.0 Mg ha−1 and 1387.4 kg ha−1 in the premature stand. The tree layer comprised 91.8 and 89.4% of the total biomass C and N pools in the young stand, and 98.0 and 95.6% in the premature stand. Total ecosystem C and N pools were, respectively, 101.4 and 4.6 Mg ha−1 for the young stand, and 260.2 and 6.6 Mg ha−1 for the premature stand. Soil C comprised 23.8–29.6% of total ecosystem C whereas soil N comprised 76.9–84.4% of the total. Our results suggest that a very high percentage of N in this subtropical forest ecosystem is stored in the mineral soil, whereas the proportion of organic C in the soil pool is more variable. The subtropical forest in eastern China seems to rapidly accumulate biomass during secondary succession, which makes it a potentially rapid accumulator of, and large sink for, atmospheric C.  相似文献   

10.
A trial in an 11-year-old stand of radiata pine (Pinus radiata D. Don) was used to analyse the effects of accelerated loss of nutrients from the site on forest productivity and nutrient status. Raking of litter was undertaken over 14 years prior to thinning, then for 2 years after thinning at which time the trial was destroyed in a wind storm. The experimental design was a factorial of three main treatments: (i) removal (raking) versus nil removal of the forest floor, (ii) replacement or non replacement of nutrients to adjust for imbalances between nutrients in litter and those in the tree stem, and (iii) complete replacement (or not) of all nutrients removed in the litter. Additionally, a small trial was incorporated to address components of physical aspects of litter removal by comparing raking with ‘raking and a cover of woven plastic mesh’. Raking and nutrient additions were carried out approximately every 6 months.Over the study period, the raking treatment removed about 75 Mg ha−1 of organic material with contained nutrients (559 kg ha−1 of N, 68 kg ha−1 of P, 323 kg ha−1 of Ca, 91 kg ha−1 of Mg, 243 kg ha−1 of K, 0.9 kg ha−1 of B) and this related to about four normal sawlog harvests or one total tree harvest. Up to the time of thinning, raking reduced basal area increment by 25% while raking together with replacement of nutrients reduced this by about 12%. Nutrient additions to unraked plots led to increases of up to 14% in basal area increment. The raking treatment reduced foliage nitrogen and this was correlated with reduced growth while other nutrients such as boron and sulphur were reduced but not to a degree to affect growth or health. The results were used to assess the effects on soil nutrient status and growth of different harvesting regimes (wood only, wood plus bark, total tree).  相似文献   

11.
Clear-cutting followed by mechanical site preparation is the major disturbance influencing nutrient and water fluxes in Fennoscandian boreal forests. The effects of soil harrowing on the fluxes of dissolved organic carbon (DOC), dissolved nitrogen compounds (organic N, NH4+ and NO3) and water soluble phosphorus (PO43−) through a podzolic soil were studied in a clear-cut in eastern Finland for 5 years. The old, mixed coniferous stand was clear-cut and stem only harvested in 1996 followed by soil harrowing in 1998 and planting in June 1999. Zero-tension lysimeters were used to collect soil water from below different soil horizons in the three types of microsites that resulted from site preparation treatment: low ridges (25% of clear-cut area), shallow furrows (30%) and the undisturbed soil (45%). After soil harrowing, the leaching of DOC, N and P from below the B-horizon increased compared to pre-treatment levels. However, the increases were short-lasting; 1–2 years for inorganic N and P, and 5 years for DOC and organic N. The highest concentrations were associated with the ridges and lowest with the furrows, reflecting the differences in amount of organic matter present in each microsite type and, for N, to enhanced mineralization and nitrification. Leaching from below the B-horizon over the 5 years following soil harrowing for the whole clear-cut area was 36.5 kg ha−1 for DOC, 0.88 kg ha−1 for NH4-N, 0.46 kg ha−1 for NO3-N, 1.24 kg ha−1 for organic N and 0.09 kg ha−1 for PO4-P. Site preparation increased temporarily the risk for nutrient leaching into watercourses and groundwater from the clear-cut area but soil fertility was not affected since the leached amounts remained small. The main reasons for the observed low leaching values were the rapid recovery of ground vegetation and low N deposition loads.  相似文献   

12.
Nitrogen (N) deposition exceeds the critical loads for this element in most parts of Switzerland apart from the Alps. At 17 sites (8 broadleaved stands, 8 coniferous stands, and 1 mixed stand) of the Swiss Long-term Forest Ecosystem Research network, we are investigating whether N deposition is associated with the N status of the forest ecosystems. N deposition, assessed from throughfall measurements, was related to the following indicators: (1) nitrate leaching below the rooting zone (measured on a subset of 9 sites); (2) the N nutrition of the forest stand based on foliar analyses (16 sites); and (3) crown defoliation, a non specific indicator of tree vitality (all 17 sites). Nitrate leaching ranging from about 2 to 16 kg N ha−1 a−1 was observed at sites subjected to moderate to high total N deposition (>10 kg ha−1 a−1). The C/N ratio of the soil organic layer, or, when it was not present, of the upper 5 cm of the mineral soil, together with the pool of organic carbon in the soil, played a critical role, as previous studies have also found. In addition, the humus type may need to be considered as well. For instance, little nitrate leaching (<2 kg N ha−1 a−1) was recorded at the Novaggio site, which is subjected to high total N deposition (>30 kg ha−1 a−1) but characterized by a C/N ratio of 24, large organic C stocks, and a moder humus type. Foliar N concentrations correlated with N deposition in both broadleaved and coniferous stands. In half of the coniferous stands, foliar N concentrations were in the deficiency range. Crown defoliation tended to be negatively correlated with N concentrations in the needles. In the majority of the broadleaved stands, foliar N concentrations were in the optimum nutritional range or, on one beech plot with high total N deposition (>25 kg ha−1 a−1), above the optimum values. There was no correlation between the crown defoliation of broadleaved trees and foliar concentrations.  相似文献   

13.
Significant increases in aboveground biomass production have been observed when Eucalyptus is planted with a nitrogen-fixing species due to increased nutrient availability and more efficient use of light. Eucalyptus and Acacia are among the most popular globally planted genera with the area of Eucalyptus plantations alone expanding to over 19 Mha over the past two decades. Despite this, little is known about how nutrition and light availability in mixed-species tree plantations influence water use and water use efficiency (WUE). This study examined to what extent water use and WUE have been influenced by increased resource availability and growth in mixed-species plantations. Monocultures of Eucalyptus globulus Labill. and Acacia mearnsii de Wildeman and 1:1 mixtures of these species were planted. Growth and transpiration were measured between ages 14 and 15 years. Aboveground biomass increment (Mg ha−1) was significantly higher in mixtures (E. globulus; 4.8 + A. mearnsii; 0.9) than E. globulus (3.3) or A. mearnsii monocultures (1.6). Annual transpiration (mm) measured using the heat pulse technique was also higher in mixtures (E. globulus; 285 + A. mearnsii; 134) than in E. globulus (358) and A. mearnsii (217) monocultures. Mixtures exhibited higher WUE than monocultures due to significant increases in the WUE of E. globulus in mixtures (1.69 kg aboveground biomass per cubic metre water transpired) compared to monocultures (0.94). The differences in WUE appear to result from increases in canopy photosynthetic capacity and above- to belowground carbon allocation in mixtures compared to monocultures. Although further studies are required and operational issues need to be resolved, the results of this study suggest that mixed eucalypt–acacia plantations may be used in water-limited environments to produce a given amount of wood with less water than eucalypt monocultures. Alternatively, because mixtures can be more productive and use more water per unit land area (but use it more efficiently), they could be utilized in recharge zones where rising water tables and salinity result from the replacement of vegetation (fast growing trees) that uses higher quantities of water with vegetation (shallow rooted annual crops) that use lower quantities of water.  相似文献   

14.
Nitrogen fertilization increased largely over the last decade in tropical eucalypt plantations but the behaviour of belowground tree components has received little attention. Sequential soil coring and ingrowth core methods were used in a randomized block experiment, from 18 to 32 months after planting Eucalyptus grandis, in Brazil, in order to estimate annual fine root production and turnover under contrasting N fertilization regimes (120 kg N ha−1 vs. 0 kg N ha−1). The response of growth in tree height and basal area to N fertilizer application decreased with stand age and was no longer significant at 36 months of age. The ingrowth core method provided only qualitative information about the seasonal course of fine root production and turnover. Mean fine root biomasses (diameter <2 mm) in the 0–30 cm layer measured by monthly coring amounted to 0.91 and 0.84 t ha−1 in the 0 N and the 120 N treatments, respectively. Fine root production was significantly higher in the 0 N treatment (1.66 t ha−1 year−1) than in the 120 N treatment (1.12 t ha−1 year−1), probably as a result of the greater tree growth in the control treatment throughout the sampling period. Fine root turnover was 1.8 and 1.3 year−1 in the 0 N and the 120 N treatments, respectively. However, large fine root biomass (diameter <1 mm) was found down to a depth of 3 m one year after planting: 1.67 and 1.61 t ha−1 in the 0 N and the 120 N treatments, respectively. Fine root turnover might not be insubstantial in deep soil layers where large changes in soil water content were observed.  相似文献   

15.
Studies on the combined effects of beech–spruce mixtures are very rare. Hence, forest nutrition (soil, foliage) and nutrient fluxes via throughfall and soil solution were measured in adjacent stands of pure spruce, mixed spruce–beech and pure beech on three nutrient rich sites (Flysch) and three nutrient poor sites (Molasse) over a 2-year period. At low deposition rates (highest throughfall fluxes: 17 kg N ha−1 year−1 and 5 kg S ha−1 year−1) there was hardly any linkage between nutrient inputs and outputs. Element outputs were rather driven by internal N (mineralization, nitrification) and S (net mineralization of organic S compounds, desorption of historically deposited S) sources. Nitrate and sulfate seepage losses of spruce–beech mixtures were higher than expected from the corresponding single-species stands due to an unfavorable combination of spruce-similar soil solution concentrations coupled with beech-similar water fluxes on Flysch, while most processes on Molasse showed linear responses. Our data show that nutrient leaching through the soil is not simply a “wash through” but is mediated by a complex set of reactions within the plant–soil system.  相似文献   

16.
Contrasting responses of Eucalyptus trees to K fertilizer applications have been reported on soils with low K contents. A complete randomized block experiment was set up in Brazil to test the hypothesis that large atmospheric deposits of NaCl in coastal regions might lead to a partial substitution of K by Na in Eucalyptus physiology and enhance tree growth. Treatments with application of 1.5, 3.0, 4.5 kmol K ha−1 (K1.5, K3.0, K4.5, respectively) as KCl, 3.0 kmol K ha−1 applied as K2SO4, 3.0 kmol Na ha−1 (Na3.0) as NaCl commercialized for cattle feeding, and a mixture of 1.5 kmol K + 1.5 kmol Na ha−1 (K1.5 + Na1.5) were compared to a control treatment (C) with no K and Na applications. All the plots were fertilized with large amounts of the other nutrients.  相似文献   

17.
Kanzler  Michael  Böhm  Christian  Freese  Dirk 《New Forests》2021,52(1):47-68

The aim of this study was to evaluate the potential of short rotation alley cropping systems (SRACS) to improve the soil fertility of marginal post-mining sites in Brandenburg, Germany. Therefore, we annually investigated the crop alleys (AC) and black locust hedgerows (ABL) of a SRACS field trail under initial soil conditions to identify the short-term effects of tree planting on the storage of soil organic carbon (SOC) and its degree of stabilization by density fractionation. We detected a significant increase in SOC and hot-water-extractable organic C (HWEOC) at ABL, which was mainly restricted to the uppermost soil layer (0–10 cm). After 6 years, the SOC and HWEOC accumulation rates at ABL were 0.6 Mg and 46 kg ha?1 year?1, which were higher than those in the AC. In addition, comparatively high stocks of approximately 4.6 Mg OC and 182 kg HWEOC ha?1 were stored in the ABL litter layer. Density fractionation of the 0–3 cm soil layer at ABL revealed that the majority of the total SOC (47%) was stored in the free particulate organic matter fraction, which was more than twice that of the AC. At the same time, a higher and steadily increasing amount of SOC was stored in the occluded particulate organic matter fraction at ABL, which indicated a high efficiency for SOC stabilization. Overall, our findings support the suitability of black locust trees for increasing the soil fertility of the reclaimed mining substrate and, consequently, the high potential for SRACS to serve as an effective recultivation measure at marginal sites.

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18.

Fineroots (≤ 2 mm diameter) are dynamic components of the forest ecosystems and play important role in water and nutrient acquisition in forests. These roots are sensitive to forest fertilization and therefore, the response of fineroots to nutrient application would provide better understanding of the forest carbon and nutrient dynamics that will be helpful in sustainable forest management plans. Two fertilization treatments, including (1) F400: 400 g P2O5 (16.5%)/tree and (2) F600: 600 g P2O5 /tree, and F0: control (without fertilization), were applied in an Acacia mangium plantation with a planting density of 1100 trees/ha (3 m?×?3 m). The evaluation of fineroot growth across seasons showed that fertilization significantly increased production and subsequent mortality and decomposition. The total decomposition associated with F600 application was 7.95 tons ha?1 year?1, equaling 121% of F400 and 160% of the control. Mortality in F600 was 8.75 tons ha?1 year?1, equaling 111% of F400 and 198% of F0, while production in F600 was 10.40 tons ha?1 year?1, equaling 127% of F400 and 143% of F0. Fineroot production, mortality, and decomposition are seasonally dependent, with higher values measured in the rainy season than in the dry season. Stand basal area increment was significantly correlated with fineroot production (R2?=?0.75), mortality (R2?=?0.44), and decomposition (R2?=?0.48). This study showed that fertilization could facilitate fineroot production, which can then lead to a higher turnover of carbon and nutrients through the decomposition of the greater mass of the fineroots.

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19.
Microbial communities play a pivotal role in soil nutrient cycling, which is affected by nitrogen loading on soil fungi and particularly mycorrhizal fungi. In this experiment, we evaluated the effects of allochthonous nitrogen addition on soil bacteria and fungi in two geographically distinct but structurally similar scrub oak forests, one in Florida (FL) and one in New Jersey (NJ). We applied allochthonous nitrogen as aqueous NH4NO3 in three concentrations (0 kg ha−1 yr−1 (deionized water control), 35 kg ha−1 yr−1 and 70 kg ha−1 yr−1) via monthly treatments over the course of 1 yr. We applied treatments to replicated 1 m2 plots, each at the base of a reference scrub oak tree (Quercus myrtifolia in FL and Q. ilicifolia in NJ). We measured microbial community response by monitoring: bacterial and fungal biomass using substrate induced respiration, and several indicators of community composition, including colony and ectomycorrhizal morphotyping and molecular profiling using terminal restriction fragment length polymorphism (TRFLP). Bacterial colony type richness responded differently to nitrogen treatment in the different sites, but ectomycorrhizal morphotype richness was not affected by nitrogen or location. Both experimental sites were dominated by fungi, and FL consistently supported more bacterial and fungal biomass than NJ. Bacterial biomass responded to nitrogen addition, but only in FL. Fungal biomass did not respond significantly to nitrogen addition at either experimental site. The composition of the bacterial community differed between nitrogen treatments and experimental sites, while the composition of the fungal community did not. Our results imply that bacterial communities may be more sensitive than fungi to intense pulses of nitrogen in sandy soils.  相似文献   

20.
Tree-based intercropping (TBI) systems, combining agricultural alley crops with rows of hardwood trees, are largely absent in Canada. We tested the hypothesis that the roots of 5–8 years old hybrid poplars, growing in two TBI systems in southern Québec, would play a “safety-net” role of capturing nutrients leaching below the rooting zone of alley crops. TBI research plots at each site were trenched to a depth of 1 m on each side of an alley. Control plots were left with tree roots intact. In each treatment at each site, leachate at 70 cm soil depth was repeatedly sampled over two growing seasons using porous cup tension lysimeters, and analyzed for nutrient concentrations. Daily water percolation rates were estimated with the forest hydrology model ForHyM. Average nutrient concentrations for all days between consecutive sampling dates were multiplied by water percolation rates, yielding daily nutrient leaching loss estimates for each sampling step. We estimated that tree roots in the TBI system established on clay loam soil decreased subsoil NO3 leaching by 227 kg N ha−1 and 30 kg N ha−1 over two consecutive years, and decreased dissolved organic N (DON) leaching by 156 kg N ha−1 year−1 in the second year of the study. NH4 + leaching losses at the same site were higher when roots were present, but were 1–2 orders of magnitude lower than NO3 or DON leaching. At the sandy textured site, the safety net role of poplar roots with respect to N leaching was not as effective, perhaps because N leaching rates exceeded root N uptake by a wider margin than at the clay loam site. At the sandy textured site, significant and substantial reductions of sodium leaching were observed where tree roots were present. At both sites, tree roots reduced DON concentrations and the ratio of DON to inorganic N, perhaps by promoting microbial acquisition of DON through rhizodeposition. This study demonstrated a potential safety-net role by poplar roots in 5–8 year-old TBI systems in cold temperate regions.  相似文献   

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