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1.
The effects of three fungicidal dusts (captan ; a mixture of benomyl, thiram and streptomycin sulfate; and a mixture of chloroneb, thiram and streptomycin sulfate) on the short-term populations dynamics of fungi and bacteria associated with caladium seedpieces planted in raw muck soil were investigated. Both germinating seedpieces and seedpieces which were decomposing after the removal of eyes were used. To determine microbial populations, dilutions of a comminuted suspension of a seed-piece and surrounding soil were plated on selective media at 0,2,4, 8 and 12 weeks after seedpieces were planted. Addition of seedpieces to soil resulted in increases in populations in the following approximate chronological order: fluorescent Pseudomonas spp, Pythium spp, total bacteria, Fusarium spp and other genera of fungi. Most organisms assayed reached higher populations on decomposing than on germinating seedpieces. The effects of the fungicidal dusts on populations of microorganisms associated with seedpieces were not related to changes in populations that followed incorporation of the dusts into soil without seedpieces. The fungicidal dusts reduced total fungal population increases on germinating, but not on decomposing, seedpieces. Bacterial population increases were similar for all treatments of germinating seedpieces, but were prolonged on decomposing, fungicide-dusted seedpieces, as compared to controls.  相似文献   

2.
The influence of potato cultivar and soil type on effectiveness of plant growth-promoting rhizobacteria (PGPR) was examined. Rhizobacteria were isolated from potato roots and tubers obtained from fields with a history of high potato yields. Fluorescent pigment-producing rhizobacteria. identified as strains of Pseudomonas putida and P. fluorescens, were selected for their antibiosis against Erwinia carovotora ssp. carotovora and growth-promoting activity on potatoes. In greenhouse tests, treatments of potato seedpieces and stem cuttings increased shoot dry weight from 1.23- to 2.00-fold and root dry weight from 1.27- to 2.78-fold. Survival of PGPR in the rhizosphere was monitored using antibioticresistant strains. Populations of these strains decreased from 3.6 × 109 cgu g?1 dry root weight to 4.5 × 105 cfu g?1 dry root weight 4 weeks after treatment. In field trials, PGPR strains were applied to seedpieces of cultivars Kennebec, Pungo, Red Pontiac and Superior and planted in Cape Fear loam. Plymouth loamy sand or Delanco sandy loam. Significant yield increases of 1.17–1.37-fold over controls were observed in two of three field trials. Variability in plant growth-promoting activity was observed between greenhouse and field trials, and no given treatment combination of PGPR strain, potato cultivar and soil type was consistently better than another.  相似文献   

3.
The effects of biocide use on nontarget organisms, such as arbuscular mycorrhizal (AM) fungi, are of interest to agriculture, since inhibition of beneficial organisms may counteract benefits derived from pest and disease control. Benomyl, pentachloronitrobenzene (PCNB) and captan were tested for their effects on the germination and early hyphal growth of the AM fungiGlomus etunicatum (Becker & Gerd.),Glomus mosseae (Nicol. & Gerd.). Gerd. and Trappe andGigaspora rosea (Nicol & Schenck) in a silty-clay loam soil placed in petri plates. Application of fungicides at 20 mg active ingredient (a.i) kg?1 soil inhibited spore germination by all three AM-fungal isolates incubated on unsterilized soil for 2 weeks. However, fungicides applied at 10 mg a.i. kg?1 soil had variable effects on AM-fungal isolates. Fungicide effects on germination and hyphal growth of G.etunicatum were modified by soil pasteurization and CO2 concentration in petri plates and also by placing spores below the soil surface followed by fungicide drenches. Effects of fungicides on mycorrhiza formation and sporulation of AM fungi, and the resulting host-plant response, were evaluated in the same soil in associated pea (Pisum sativum L.) plants. Fungicides applied at 20 mg a.i. kg?1 soil did not affect the root length colonized byG. etunicatum, but both benomyl and PCNB reduced sporulation by this fungus. Benomyl and PCNB reduced the root length colonized byG. rosea at 48 and 82 days after transplanting. PCNB also reducedG. mosseae-colonized root length at 48 and 82 days, but benomyl only affected root length colonized byG. mosseae at the earlier time point. Only PCNB reduced sporulation byG. mosseae, consistent with its effect on root length colonized by this fungus. captan reduced the root length colonized by G. rosea at 48 days, but not at 82 days, and reduced colonization byG. mosseae at 82 days, but not at 48 days. Captan did not affect sporulation by any of the fungi.G. rosea spore production was highly variable, but benomyl appeared to reduce sporulation by this fungus. Overall,G. etunicatum was the most tolerant to fungicides in association with pea plants in this soil, andG. rosea the most sensitive. Benomyl and PCNB were overall more toxic to these fungi than captan. Interactions of AM fungi and fungicides were highly variable and biological responses depended on fungus-fungicide combinations and on environmental conditions.  相似文献   

4.
This investigation was undertaken to study the compatibility of Mesorhizobium sp. Cicer with captan (fungicide) and chlorpyrifos (insecticide) for growth, symbiotic parameters and yield in chickpea. In an in vitro experiment, a significant reduction in the number of viable mesorhizobia was observed in Mesorhizobium sp. Cicer treated chickpea seeds at the recommended doses of captan (3 g kg?1 seed) and chlorpyrifos (10 ml kg?1 seed) after 4 h storage at 4°C, and further reduction was seen after 8–16 h contact with Mesorhizobium. The results showed that captan was more toxic than chlorpyrifos. In field experiments, improved growth and symbiotic parameters (plant height, dry weight of shoot, nodulation, leghaemoglobin content, chlorophyll content and nitrogen content) and a reduction in per cent damaged by termites and diseases were observed in the Mesorhizobium alone treatment compared with the uninoculated control. Grain yield was increased significantly in treatments with Mesorhizobium alone or in a mixture with fungicide and insecticide (captan and chlorpyrifos) compared with the control treatment. It is evident that chemically treated seeds should always be sown as soon as possible after inoculation. Recommended rates of captan and chlorpyrifos application with Mesorhizobium inoculant as a seed treatment was innocuous to chickpea–Mesorhizobium symbiosis.  相似文献   

5.
The effect of temperature on interactions between combinations of the solvent acetone and the fungicide captan was determined using the fungi Pythium uhimum, Sclerotinia homeocarpa, and Pestalotia sp. Seven concentrations of acetone, ranging from 0.1 to 3.0% (v/v), were interacted with four concentrations of captan, ranging from 1.0 to 10.0 ppm (mg L?1 ). This procedure was repeated at 15, 20, 25, and 30 °C using potato dextrose agar at pH 5.5. Acetone and captan interacted synergistically towards P. ultimum and S. homeocarpa, and antagonistically towards Pestalotia sp., regardless of the temperature. The exact temperature response was dependent upon both the captan level and culture used. As the temperature increased from 15 to 30 °C, the toxicity of captan decreased by up to 40% with P. ultimum, and from < 10 to 20 with S. homeocarpa and Pestalotia sp. With only a few exceptions, the magnitude of interactions observed generally decreased as the temperature was increased from 15 to 30 °C. This was most pronounced with P. ultimum. Generally, the lowest interaction magnitudes were recorded at 20 °C for P. ultimum, 20 to 25 °C for S. homeocarpa, and 30 °C for Pestalotia sp. The greatest interaction magnitudes were usually obtained at 15 or 30 °C with P. ultimum, 15 °C with S. homeocarpa, and 25 °C with Pestalotia sp.  相似文献   

6.
The effects of biocide use on nontarget organisms, such as arbuscular mycorrhizal (AM) fungi, are of interest to agriculture, since inhibition of beneficial organisms may counteract benefits derived from pest and disease control. Benomyl, pentachloronitrobenzene (PCNB) and captan were tested for their effects on the germination and early hyphal growth of the AM fungiGlomus etunicatum (Becker & Gerd.),Glomus mosseae (Nicol. & Gerd.). Gerd. and Trappe andGigaspora rosea (Nicol & Schenck) in a silty-clay loam soil placed in petri plates. Application of fungicides at 20 mg active ingredient (a.i) kg–1 soil inhibited spore germination by all three AM-fungal isolates incubated on unsterilized soil for 2 weeks. However, fungicides applied at 10 mg a.i. kg–1 soil had variable effects on AM-fungal isolates. Fungicide effects on germination and hyphal growth of G.etunicatum were modified by soil pasteurization and CO2 concentration in petri plates and also by placing spores below the soil surface followed by fungicide drenches. Effects of fungicides on mycorrhiza formation and sporulation of AM fungi, and the resulting host-plant response, were evaluated in the same soil in associated pea (Pisum sativum L.) plants. Fungicides applied at 20 mg a.i. kg–1 soil did not affect the root length colonized byG. etunicatum, but both benomyl and PCNB reduced sporulation by this fungus. Benomyl and PCNB reduced the root length colonized byG. rosea at 48 and 82 days after transplanting. PCNB also reducedG. mosseae-colonized root length at 48 and 82 days, but benomyl only affected root length colonized byG. mosseae at the earlier time point. Only PCNB reduced sporulation byG. mosseae, consistent with its effect on root length colonized by this fungus. captan reduced the root length colonized by G. rosea at 48 days, but not at 82 days, and reduced colonization byG. mosseae at 82 days, but not at 48 days. Captan did not affect sporulation by any of the fungi.G. rosea spore production was highly variable, but benomyl appeared to reduce sporulation by this fungus. Overall,G. etunicatum was the most tolerant to fungicides in association with pea plants in this soil, andG. rosea the most sensitive. Benomyl and PCNB were overall more toxic to these fungi than captan. Interactions of AM fungi and fungicides were highly variable and biological responses depended on fungus-fungicide combinations and on environmental conditions.  相似文献   

7.
Seed-applied fungicides are commonly used to prevent or suppress fungal disease organisms in pulse crop production. However, non-target beneficial fungi, such as arbuscular mycorrhizal fungi (AMF), also may be affected. Seed-applied fungicides Agrox® FL (active ingredient: captan), Allegiance™ FL (metalaxyl), Apron Maxx® RTA® (fludioxonil and metalaxyl), Thiram 75WP (thiram), Vitaflo® 280 (carbathiin and thiram), Crown® (carbathiin and thiabendazole), and Trilex® AL (trifloxystrobin and metalaxyl) were assessed in a greenhouse study for their effects on colonization and development of AMF in pea and chickpea, and the consequent impact on plant growth. In the absence of disease pressure, systemic fungicides Allegiance™ FL, Apron Maxx® RTA®, Vitaflo® 280, Crown® and Trilex® AL restricted mycorrhizal colonization, host growth and P uptake to different levels. In contrast, contact fungicides Agrox® FL and Thiram 75WP had minimal effects on mycorrhizal colonization, host growth and P uptake. Although consequent sporulation and glomalin-related protein production were not significantly affected by fungicides at an early host growth stage, the compositional structure of the AMF community in host roots was significantly altered in response to Agrox® FL, Allegiance™ FL, Apron Maxx® RTA®, and Trilex® AL as revealed by pyrosequencing-based analysis of fungal 18S rRNA. These results indicate that the suppressive effects of seed-applied fungicides on AMF development depend on specific fungicide-AMF interactions.  相似文献   

8.
 We assessed the effects of the fungicides captan and thiram on the survival and phenotypic characteristics of Rhizobium leguminosarum bv. viceae, strain C1. Fungicide was applied to pea seed at rates of 0.25–2 g a.i. kg–1 seed, and treated seed was inoculated with strain C1. After 24 h, rhizobia were extracted from treated seed and viable numbers determined by plating onto yeast extract mannitol agar. Phenotypic characteristics were assessed using FAME (fatty acid methyl ester) profiles and Biolog substrate utilization patterns. Captan and thiram significantly reduced the numbers of rhizobia recovered from seed and altered the FAME and Biolog profiles of recovered rhizobia. However, only the highest concentrations of captan affected nodulation and plant growth. Contact with some seed-applied fungicides can significantly alter phenotypic characteristics of rhizobia, but these changes might be offset by the presence of host plants. Received: 20 February 1999  相似文献   

9.
As a result of repeated applications, some fungicides may accumulate in the soil to levels high enough to have adverse effects on the activity of soil microorganisms and plant growth. Comparison of the effects of 10 mg kg‐1 soil of the benlate, captan, and lime‐sulfur fungicides with the nitrification inhibitors (NI) nitrapyrin and terrazole on oxidation of NH4 + in Tifton loamy sand (siliceous, thermic plinthic Typic Kandiudults) incubated at 30° C showed that benlate had no significant effects whereas captan inhibited nitrification 21% more than lime‐sulfur, but about 20% less than NI. Application of benlate enhanced NO3 reduction to N2O and N2 in liquid medium inoculated with soil whereas 50 and 100 mg L"1 medium of captan and lime‐sulfur compared favorably with the NI in suppressing NO3 and NO2 reductions, but were less effective than the inhibitors when applied at the low rate of 10 mg L‐1 medium. In a greenhouse study with tomato (Lycopersicon esculentum Mill. cv. ‘Better Boy'), weekly drench applications of 0.25 mg kg‐1 soil of the test biocides for four weeks with three NH4 +‐N: NO3 ‐N ratios showed that benlate applied with 1: 0 N ratio and lime‐sulfur applied with 0: 1 N ratio restricted significantly the plant growth and N uptake. The largest root: shoot ratios, total plant dry weight, and N uptake were obtained with plants fertilized with 1: 1 N ratio in combination with the biocides.  相似文献   

10.
Four fungicides, Captan, Dicloran, Thiram and Verdasan were applied at 28-day intervals for 12 consecutive months and their effects on soil fungal numbers and the incidence of individual species were studied. Immediately after application, these fungicides reduced the number of fungal propagules in soil by 23, 11, 36 and 50% respectively compared with control. Captan- and Dicloran-treated soils were rapidly recolonised within 7 days of the application of fungicides. The effects of Thiram and Verdasan were more persistent: the fungal numbers in soils treated with these fungicides did not recover sufficiently to reach control levels throughout the sampling period. Fewer species of fungi were isolated from Thiram- and Verdasan-treated soils than from Captan- and Dicloran-treated soils. Chrysosporium pannorum (Link) Hughes was the principal species isolated from Verdasan-treated soil and the fungus was isolated in increased numbers immediately after application of Verdasan than on subsequent sampling days. Cladosporium cladosporioides (Fres.) de Vries, Mortierella minutissima van Tiegh, Trichocladium asperum Harz, Trichoderma hamatum (Bon.) Bain, and Zygorhynchus moelleri Vuill. were found to be generally tolerant of all four fungicides. However, Botryotrichum piluliferum Sacc. and Marchai, Gliocladium roseum Bain., Humicola fusco-atra Traaen, Sepedonium chrysospermum (Bull.) Link ex Fr., and Trichoderma viride Pers. ex Fr. were generally intolerant of the fungicides but rapidly recolonised the treated soils. While the concentrations of Captan and Dicloran used were fungistatic to T. viride, Thiram and Verdasan were fungicidal.  相似文献   

11.
The effect of pH on interactions between combinations of the solvent acetone and the pesticide captan was determined using the fungiPythium ultimum, Sclerotinia homeocarpa, andPestalotia sp.. Seven concentrations of the solvent acetone, ranging from 0.1 to 3.0 % (v/v), were interacted with four concentrations of the fungicide captan, ranging from 1.0 to 10.0 ppm (mg L?1). This interaction procedure was repeated at pH 4.5, 5.5, 6.5, and 7.5, using a temperature of 30 °C. Acetone and captan interacted synergistically towardsP. ultimum andS. homeocarpa, and antagonistically towardsPestalotia sp., regardless of the pH. However, the solvent concentration at which synergism or antagonism was first observed usually decreased as pH increased. The actual pH response obtained was dependent upon both the captan level and culture used. As pH increased from 4.5 to 7.5, the toxicity of captan decreased by up to 40% withS. homeocarpa andPestalotia sp., and 80% withP. ultimum. WithS. homeocarpa andPestalotia sp., the magnitude of synergism or antagonism increased as the captan concentration was raised from 1.0 or 2.5 ppm up to 7.5 or 10.0 ppm. With P. ultimum, the degree of synergism decreased at pH 4.5 and 5.5, but increased at pH 6.5 and 7.5, as the captan concentration was raised from 2.5 to 10.0 ppm. The lowest interaction magnitudes were recorded at pH 4.5 forP. ultimum, but was variable for the other cultures. The greatest interaction magnitudes were obtained at pH 4.5 forS. homeocarpa, 5.5 forPestalotia sp., and 6.5 or 7.5 forP. ultimum.  相似文献   

12.
Fresh strawberries and grapes grown in Michigan and Indiana were surveyed for residues of captan and folpet, 2 fungicides commonly used on these crops. The fungicides were reportedly applied to the crops by overhead irrigation, tractor sprayer, or aerial spraying, in amounts ranging from 0.5 to 6 lb formulation/acre for captan and from 1 to 4 lb formulation/acre for folpet. Reported dates of last application ranged from just 2 days to nearly 5 months before samples were collected. Twenty-eight strawberry samples and 24 grape samples were collected of crops field-treated with one or both of these fungicides. Samples were analyzed by previously described methodology. Captan residues were found in all strawberry samples, ranging from less than 0.01 to 1.5 ppm. Folpet was found in only one strawberry sample at 0.041 ppm. Captan residues were found in only 6 grape samples, ranging from less than 0.01 to 0.082 ppm. Folpet residues were found in 12 grape samples, ranging from less than 0.01 to 0.50 ppm. All residues were well below the current tolerances of 25 ppm for both captan and folpet in strawberries and 50 ppm for captan and 25 ppm for folpet in grapes. Residue levels of these surface-applied, nonsystemic fungicides were inconsistent with amounts and dates of application, most likely because of variations in weather conditions, especially rainfall. Residues were quite stable in frozen sample homogenates, declining only 5-10% after 2 months.  相似文献   

13.
Respiratory methods to estimate the amount of C in the soil microbial biomass and the relative contributions of procaryotes and eucaryotes to the biomass were used to evaluate the influence of pesticides on the soil microflora. Experiments were conducted with 5 and 50 μg·g?1 of three fungicides, captan, thiram and verdasan. At 5 μg·g?1 they caused significant decreases (40%) in the biomass; the organomercury fungicide verdasan also caused a shift from fungal to bacterial dominance. Within 8 days, biomass in captan- and thiram-amended soils had recovered to that of the controls. Although the fungal to bacterial balance was restored in verdasan-amended soils, biomass recovery was not complete. At 50 μg·g?1 the fungicides caused long-term decreases in the biomass and altered the relative proportions of the bacterial and fungal populations. Verdasan had the greatest effect on soil microbial biomass and composition.  相似文献   

14.
The effects of seven insecticides and six fungicides on denitrification of nitrate in soils were studied by determining the effects of 10 and 50μgg?1 soil of each pesticide on the amounts of nitrate lost and the amounts of nitrite, N2O and N2 produced when soil samples were incubated anaerobically after treatment with nitrate. The insecticides used were lindane, fenitrothion, fonofos, malathion, phorate, terbufos and carbofuran. The fungicides used were mancozeb, maneb, thiram, benomyl, captan and terrazole.None of the insecticides studied had a significant effect on denitrification when applied at the rate of 10 μgg?1 soil. When applied at the rate of 50μgg?1 soil, lindane, fonofos and malathion enhanced denitrification in the three soils studied, whereas fenitrothion, phorate, terbufos and carbofuran either had no appreciable effect on denitrification in these soils, or enhanced denitrification in at least one of the soils.None of the fungicides studied had an appreciable effect on denitrification when applied at the rate of 10μgg?1 soil, but thiram increased the ratio of N2 to N2O in the gaseous products of denitrification. Captan inhibited denitrification in two of three soils studied when applied at the rate of 50μgg?1 soil. The other five fungicides either had no significant effect on denitrification, or enhanced denitrification, when applied at this rate. Reports that maneb, thiram and terrazole inhibit denitrification in soil were not confirmed.  相似文献   

15.
Abstract

In the current multiplication technique first-generation seed tubers produced in the field by transplanting plants raised on peat in plastic rolls from plants cultured by repetitive multiplication using tip- and stem-cuttings and truncated plants are compared with in vitro micro-plants from the aspect of obtaining optimal-sized, disease-free seed tubers.

The objective of the study is to compare the dynamics of total plant dry mass and tuber dry mass of field-grown potato plants, and analyse the effect of the year and variety. Two local late-maturing potato varieties, Ants and Vigri, were used in the study. The field experiments were carried out in 2005–2007.

Significant impact of the multiplication method and experimental year on total plant dry mass and tuber dry mass was observed. The plants multiplied in vitro from micro-cuttings produced lower total dry mass and also lower tuber dry mass per m2. The plants multiplied by tip- and stem-cuttings as well as truncated plants proved to be more adaptable to unfavourable weather conditions than in vitro plants; in a favourable year, however, the differences were insignificant. In the early phase of growth the ratio of the tuber dry mass to total plant dry mass increased more rapidly in the case of in vitro plants, whereas by the end of the growing season the relevant ratio was similar for all multiplication methods and years.

All developed multiplication methods are suitable for practical seed potato production in the field and ensure a reasonable potato crop.  相似文献   

16.
Many agrochemicals are applied postharvest on fruits and vegetables to extend their lives and preserve quality during storage, transport, and marketing. Persistence and distribution of residues on the edible portions of produce have been reported for citrus fruits, pome fruits, stone fruits, mangos, strawberries, bananas, kiwi fruits, avocados, some minor fruit commodities, and bell peppers and tomatoes. Data on the persistance and residues of the fungicides benomyl, biphenyl, sec-butylamine, captan, carbendazim, dicloran, fosetyl-aluminum, guazatine, imazalli, iprodione, metalaxyl, o-phenylphenol, prochloraz, thiabendazole, thiophanate-methyl, triadimeton, and vinclozolin, the fumigants ethylene dibromide, methyl bromide, and sulfur dioxide, the insecticides dimethoate and fenthion, the antiscald compounds diphenylamine and ethoxyquin, and the growth regulators 2,4-D and daminozide are presented and discussed.  相似文献   

17.
Ninety-four barley genotypes were used to investigate the genotypic differences in arsenic (As) uptake and translocation and their relationships with As tolerance index (TI) and translocation factor (TF). Two As treatments (300 µM and 500 µM) were applied in the initial screening and the confirmatory experiments, respectively. The results showed significant (p < 0.05) differences in tissue biomass, shoot height, root length, As TI and TF among genotypes. Based on As TI, 11 barley genotypes were selected and divided into 3 groups, i.e. tolerant, mildly tolerant and sensitive. There was more As uptake in the roots of the As tolerant genotypes, while the As sensitive genotypes contained more As in shoots, which was further proved by the greater TF. Significantly negative correlation was observed between shoot and root As concentration. The results showed that As tolerant genotypes are able to restrict the upward movement of As, thus developing their tolerance.  相似文献   

18.
The ability of Talaromyces flavus to occupy the rhizosphere of potato, cotton and eggplant and to reduce germinability of Verticillium dahliae microsclerotia (ms) was investigated. After 2 weeks the germination of microsclerotia of V. dahliae exposed to T. flavus-infested soil was 32–41% less than those exposed to soil that was not infested, depending on the soil type. With a new medium selective for T. flavus, TF medium, it was possible to monitor the populations of T. flavus in rhizosphere and non-rhizosphere soils. Significantly higher populations of T. flavus were found in rhizosphere soils than in non-rhizosphere soils of each of the test plants when T. flavus was applied as an ascospore drench to soil planted to potato, cotton or eggplant. The reduction of viable V. dahliae microsclerotia and the increase in T. flavus populations in rhizosphere soils may explain how T. flavus reduces Verticillium wilt of eggplant; further, it suggests the feasibility of testing the antagonist against V. dahliae on potato and cotton.  相似文献   

19.
Residues of benomyl (determined as carbendazim, widely accepted as MBC) and of thiabendazole in Wellspur apples were monitored after the apples were treated postharvest by immersion in a bath with each of the fungicides separately and stored under refrigeration. Whole fruit and pulp analyses were conducted over the period beginning 1 day after treatment and lasting up to 200 days afterwards. Under the conditions described, both benomyl (expressed as parts per million of carbendazim) and thiabendazole were found in the fruits in the following average amounts: 0.44 +/- 0.03 and 0.11 +/- 0.03 ppm benomyl (as carbendazim) in whole fruit and in pulp, respectively; 0.98 +/- 0.10 and 0.39 +/- 0.10 ppm thiabendazole in whole fruit and in pulp, respectively.  相似文献   

20.
There is increasing community awareness of the potential environmental risks posed by Cu-based fungicide use, which is placing increasing pressure on governments and industry to undertake risk minimisation action. However, if there is going to be a widespread move away from the use of Cu-based fungicides, logically there needs to be assurance that the alternatives pose a lower environmental risk. To that end, this study compared the effect of copper hydroxide, captan and trifloxystrobin on soil enzymatic (phosphomonoesterase and urease) activity. Compared to an untreated control, copper did not inhibit either enzyme activity, even at the highest dose used in the study (156 mg/kg). At their respective high doses, captan (96 mg/kg) and trifloxystrobin (144 mg/kg) did not cause inhibition of phosphomonoesterase activity but did inhibit urease activity. Consequently, the results from this study suggest that the copper hydroxide alternatives, captan and trifloxystrobin, do not pose a short-term risk to P cycling processes in soil, although the results do suggest that these two are more toxic than copper hydroxide to N cycling processes in soil. Moreover, captan and trifloxystrobin compounds are unlikely to pose a long-term risk to soil microbial function as they are unlikely to persist in soil at concentrations found to cause an adverse effect on urease activity. Nonetheless, the potential disruption to N cycling processes needs to be recognised and consideration given to limiting the annual applications of these fungicides, particularly around the timing of repeat fungicide applications, to prevent accumulation of the fungicides in surface soils.  相似文献   

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