共查询到20条相似文献，搜索用时 140 毫秒
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介绍了热泵技术的基本原理以及在国内油田地面工程领域的利用情况,包括热泵技术在联合站的采暖及伴热、在注水站的采暖及注水泵冷却、井场原油加热及其他应用等,分析了热泵技术在油田地面工程应用中存在的问题,给出了具体的方法建议。 相似文献
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总结了银露梅的特征特性,从土壤改良及整地、种子选择及处理、播种、抚育管理等方面提出了其在互助县的驯化繁育技术,并分析了其取得的成效及问题。 相似文献
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沙棘研究现状、开发利用及发展前景 总被引：10，自引：0，他引：10
介绍了沙棘的资源分布及在涵养水源、保持水土、固氮改土、防风固沙方面的作用,挖掘了沙棘在食用、药用和木材加工方面的经济价值,展望了沙棘在保持水土、治理沙漠化及深加工产品方面的发展前景。 相似文献
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本文通过对上海杨浦公园中园林地被植物(以下简称地被植物)成功应用情况及部分地被植物品种、类别、习性、用途的介绍,验证了掌握植物生态习性在园林地被植物应用过程中的重要性,列举了地被植物在乔木、花灌木树坛,花境配置及草坪改建中的应用等作为上海地区公园及公共绿地中地被植物合理应用借鉴的可行性方案。文中概括了目前地被植物应用中普遍存在的突出问题及相应解决的办法,预测了地被植物在上海应用的发展趋势。 相似文献
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Fonseca DM Keyghobadi N Malcolm CA Mehmet C Schaffner F Mogi M Fleischer RC Wilkerson RC 《Science (New York, N.Y.)》2004,303(5663):1535-1538
In the Old World, some mosquitoes in the Culex pipiens complex are excellent enzootic vectors of West Nile virus, circulating the virus among birds, whereas others bite mainly humans and other mammals. Here we show that, in northern Europe, such forms differing in behavior and physiology have unique microsatellite fingerprints with no evidence of gene flow between them, as would be expected from distinct species. In the United States, however, hybrids between these forms are ubiquitous. Such hybrids between human-biters and bird-biters may be the bridge vectors contributing to the unprecedented severity and range of the West Nile virus epidemic in North America. 相似文献
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2010年我国爆发了使蛋鸭产蛋量急剧下降、由一种鸭黄病毒引起的疾病,通过对来自山东某发病鸭场的病料进行分离,得到实验室分离株Du/CH/LSD/110128。对其进行全基因组序列测定分析,确定该株病毒全基因组含有一个开放阅读框,编码一个由3 426个氨基酸组成的多聚蛋白。将试验测得株Du/CH/LSD/110128株与黄热病毒(Yellow fever virus)、登革热病毒(Dengue virus)、伊利乌斯脑炎病毒(Ilheus virus)、西尼罗病毒(West Nilevirus)、巴格扎病毒(Bagaza virus)和其他一些已公布序列的坦布苏病毒进行核酸同源性比较和遗传进化树分析,发现Du/CH/LSD/110128株与巴格扎病毒亲缘关系较近但介于病毒种的水平上,与其他株坦布苏病毒核酸同源性在87%以上,可以确定Du/CH/LSD/110128株属于新型鸭坦布苏病毒(Duck Tembusu virus)。 相似文献
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Enserink M 《Science (New York, N.Y.)》2000,290(5496):1483
With support from the National Institutes of Health (NIH), several research groups are taking the first steps toward developing drugs to fight the acute phase of West Nile virus infection. NIH is also supporting the fast-track development of a vaccine. But because it doesn't make sense to vaccinate the entire population for such a rare disease, it is unclear how large the market for a vaccine will be. 相似文献
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间接免疫荧光检测石蜡切片中鸭肿头出血症病毒及抗原定位方法的初步建立 总被引：1，自引：0，他引：1
【目的】建立间接免疫荧光（IFA）检测石蜡切片中鸭肿头出血症病毒（DSHDV）的方法,为DSHDV感染的实验室诊断、DSHDV在感染鸭组织细胞中的亚细胞定位和动态研究提供有效的检测手段。【方法】用差速离心纯化DSHDV,将纯化DSHDV免疫家兔制备兔抗DSHDV高免血清,并以DEAE-SephadexA-50柱层析纯化出兔抗DSHDV IgG,建立IFA检测石蜡切片中DSHDV的方法;利用建立的IFA对28日龄鸭人工感染DSHDV死亡鸭不同组织器官以及临床样品进行检测。应用PAGE电泳分析DSHDV基因组特性。【结果】IFA最佳条件为：切片在0.01 mol•L-1 柠檬酸（pH 6.0）缓冲液微波修复20 min后,以10%马血清37℃下封闭30 min,然后加入1﹕50的一抗4℃孵育过夜,最后加入1﹕100 FITC标记的含0.01%伊文斯蓝的二抗37℃孵育30 min。IFA检测DSHDV感染死亡鸭肝脏石蜡切片为阳性,而检测鸭瘟、禽流感病毒(H5N1)、鸭病毒性肝炎、鸭疫里默氏杆菌感染死亡鸭肝脏石蜡切片为阴性。IFA检测28日龄人工感染DSHDV死亡鸭的不同组织器官,心、肝、脾、胰、肺、肾、法氏囊、食管、气管、胸肌、胸腺、十二指肠、空肠、回肠、盲肠和直肠为阳性;哈德氏腺、脑、皮肤、腺胃为阴性。阳性组织中病毒抗原主要分布在细胞浆。经甲醛固定保持1～6年的临床死亡鸭的病毒分离阳性肝脏,IFA检测结果也呈阳性。DSHDV具有呼肠孤病毒特征,有10条分节段核酸,呈“334”分布。【结论】建立的检测石蜡组织切片中DSHDV抗原的IFA具有直观、特异性强的优点,应用于DSHDV在感染鸭组织细胞中的亚细胞定位具有良好效果,可用于DSHDV感染的实验室诊断、病原在鸭组织细胞中分布研究。DSHDV抗原存在感染细胞浆,肠道、肾脏、法氏囊和胸腺是DSHDV侵害的主要靶器官。 相似文献
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Influenza virus effects on cell membrane proteins 总被引：16，自引：0，他引：16
During infection by influenza virus, viral proteins become firmly attached to (or part of) host cell plasma membrane, nuclear membranes, mitochondrial, and microsomal membranes. Purified virus particles contain less than I percent host cell protein. Virus envelope proteins completely replace host membrane proteins in those discrete spots on the plasma membrane from which progeny virions bud out. 相似文献
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Persistent noncytopathic infection of normal human T lymphocytes with AIDS-associated retrovirus 总被引：25，自引：0，他引：25
J A Hoxie B S Haggarty J L Rackowski N Pillsbury J A Levy 《Science (New York, N.Y.)》1985,229(4720):1400-1402
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New human T-lymphotropic retrovirus related to simian T-lymphotropic virus type III (STLV-IIIAGM) 总被引：21，自引：0，他引：21
P J Kanki F Barin S M'Boup J S Allan J L Romet-Lemonne R Marlink M F McLane T H Lee B Arbeille F Denis 《Science (New York, N.Y.)》1986,232(4747):238-243
This report describes serologic evidence for a virus similar to that known as simian T-lymphotropic virus type III of African Green monkeys (STLV-IIIAGM) infecting apparently healthy people in Senegal, West Africa, and the isolation of virus from these individuals. Serum samples from selected healthy West African people showed unusual serologic profiles when tested with antigens of HTLV-III/LAV, the etiologic agent of AIDS, and of STLV-IIIAGM. The samples reacted strongly with all of the major viral antigens of STLV-IIIAGM but showed variable or no reactivity with the major viral antigens of HTLV-III/LAV by radioimmunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A new human T-lymphotropic virus (HTLV-IV) isolated from these people was grown in vitro and shown to have retroviral type particles, growth characteristics, and major viral proteins similar to those of the STLV-III and HTLV-III/LAV group of retroviruses. The gp120/160, gp32, p64, p55, p53, p24, and p15 proteins precipitated were the same size as and reactive with STLV-IIIAGM proteins. The serologic data suggest that this virus shares more common epitopes with STLV-IIIAGM than with the prototype HTLV-III/LAV that infects people in the United States and Europe. Further study of this virus and of the origin of the HTLV-III/LAV group of viruses may expand our understanding of the human AIDS virus. 相似文献
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Lindenbach BD Evans MJ Syder AJ Wölk B Tellinghuisen TL Liu CC Maruyama T Hynes RO Burton DR McKeating JA Rice CM 《Science (New York, N.Y.)》2005,309(5734):623-626
Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10(5) infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals. 相似文献