共查询到20条相似文献，搜索用时 187 毫秒
1.
电子束辐射诱变及克隆选择培育好好芭抗寒无性系的研究 总被引：6，自引：1，他引：6
利用电子辐射处理好好芭试管苗，从改变基因型入手提高其抗寒力，以获得稳定遗传的好好芭株系。研究表明150GY的辐射量是诱发好好芭发生变异的最佳剂量，结合生化标记，抗寒相关的酶类——淀粉酶和过氧化物酶活性分析，人工模拟低温实验的克隆检测，筛选出了具有较高抗寒能力的好好芭株系，这些株系通过无性系繁殖可用于抗寒育种。 相似文献
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低温和植物生长调节物质处理对好好芭无性系试管苗抗寒相关酶类活性的影响 总被引：6，自引：1，他引：6
采用不同的低温驯化时间、不同浓度的脱落酸(ABA)和矮壮素(CCC)以及不同冰冻温度的人工模拟冰冻实验处理好好芭无性系，测定其淀粉酶活性和过氧化物酶活性。研究发现无性系间两种酶活性均有较大差异。分别在经2℃驯化7d、培养基中含lmg／L ABA或含2mg／L CCC及冰冻温度-8℃时，淀粉酶活性最大，过氧化物酶活性也有相应的变化。说明这两种酶活性变化与好好芭植株抗冻性也是相关的，利用淀粉酶和过氧化物酶作为生化指标来进行好好芭抗寒性无性系的筛选，培育抗寒品种是可行的。 相似文献
3.
干旱及冻害胁迫下好好芭抗旱和耐寒性的测定方法 总被引：2，自引：0，他引：2
采用不同模拟干旱和模拟冻害条件,以不同处理时间长度处理好好芭叶片或试管苗,分别测定其失水率和电导率.结果表明PEG模拟干旱条件下的失水率和人工模拟冻害条件下的电导率作为检测指标可以快速、客观、有效地反映好好芭生活状态. 相似文献
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综述了目前国内蓝莓组织培养技术的研究状况,介绍了蓝莓组织培养过程中不同外植体无菌体系的建立、外植体培养、试管苗生根培养等方面的技术,并对蓝莓组织培养中的问题进行了分析,最后对组织培养技术在蓝莓栽培中的应用进行了展望。 相似文献
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组织培养是植物无性繁殖和遗传转化的重要技术基础。组织培养包括外植体脱分化、愈伤组织形成、再分化等过程,最后形成新的植株。在植物组织培养过程中,DNA甲基化模式不断发生变化,表明植物细胞发生了大量的表观遗传重编程事件。一般来说,DNA甲基化可以促进或加快组织培养过程,对于许多很难进行组织培养和再生的植物来说非常重要。本文主要总结了植物组织培养过程中DNA甲基化模式的变化规律和分子机制,以及新的研究手段和思路,旨在为开展植物组织培养过程中的DNA甲基化研究提供帮助。 相似文献
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玉米组织培养及植株再生研究进展 总被引：2，自引：0，他引：2
玉米组织培养及再生,目的是让外植体能够高频率地诱导出愈伤组织,建立高效的体细胞再生体系,为玉米遗传转化和细胞工程研究创造有利条件。主要综述了玉米组织培养研究进展,并就玉米组织培养存在的问题提出了解决方法。 相似文献
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多效唑是一种植物生长延缓剂,具有延缓植物生长、抑制茎枝伸长,促进分蘖,促进成花和座果,增强抗旱及抗寒性,提高耐盐性和延缓植物衰老等多种效应。本文从组织培养、抗逆性研究、产质量研究以及残留问题等方面综述了多效唑在植物生产中的应用和研究情况,为多效唑在植物生产上的应用研究提供方便。 相似文献
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《Industrial Crops and Products》2006,23(1):46-53
There is growing interest in recovering ferulic acid from plant sources for use as feedstock for several high-value applications. Jojoba meal was examined as a potential source of ferulic acid. The feruloyl esterase domain of the Clostridium thermocellum cellulosomal xylanase was employed to hydrolyze ferulic acid from defatted jojoba meal. Esterase treatment produced 6.7 g of ferulic acid/kg of jojoba meal. The predominant source (86%) of the ferulate was found to originate from the meal's water-soluble simmondsin fraction. Seven feruloyl simmondsin species from jojoba meal were identified by liquid chromatography–mass spectroscopy. Only one species, a didemethylsimmondsin ferulate, displayed an enzymatic hydrolysis rate distinctly faster than the other feruloyl simmondsins. Complete hydrolysis of all feruloyl simmondsin species was achieved in 24–48 h at 60 °C with a 100:1 meal:enzyme weight ratio. Ferulic acid was efficiently recovered from the medium by ethyl acetate extraction. The recovered ferulic acid was readily converted to ethyl ferulate, demonstrating a facile procedure for producing a valuable product from defatted jojoba meal. 相似文献
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茶树组织培养中的褐化控制研究 总被引：1，自引：0，他引：1
自20世纪60年代末以茶树幼茎小块为材料研究了离体咖啡碱的合成以来,茶树组织培养至今已走了约50年的研究历程,在多方面得到应用,但仍然有不少难题未攻克,主要有污染、褐化、玻璃苗、难以分化、再生体系不完善等等。其中,因为茶树多酚类物质含量高,褐化现象严重,阻碍了组培的后续工作。因此,有效的控制茶树外植体褐化是茶树组织培养的关键技术。本文就目前国内茶树组织培养的褐化控制研究进行综述,对茶树组织培养有参考意义。 相似文献
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为降低马铃薯组培苗的污染率,试验对不同浓度的医用抗生素、防腐剂、化学农药的抑菌效果以及对马铃薯组培苗生长及生根的影响进行了研究。试验数据表明：70%甲基硫菌灵可湿性粉剂和50%多菌灵可湿性粉剂对马铃薯组培苗生产中最常见的青霉菌、细菌和酵母菌3种菌都具有较好抑制作用,并且对马铃薯试管苗的生长以及生根产生的负影响最小。 相似文献
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Fadhel Jaâfar Mohamed Ali Lassoued Mahdi Sahnoun Souad Sfar Morched Cheikhrouhou 《Fibers and Polymers》2012,13(3):346-351
The medical uses of textile fibers and fabric forms keep on growing. Today, the knits can be used in the medical field. The
aim of this present work was to prepare compressive knits, impregnated by ethylcellulose (EC) microcapsules, for the burnt
skin. Ethylcellulose microcapsules containing jojoba oil were prepared via the phase separation method. Jojoba oil was chosen
because it plays an essential role in skin hydration and avoids the sebum accumulation. Ethylcellulose microcapsules obtained
were grafted on two different compressive knit surfaces by impregnation, using the Acrylic Resin (AR) as binder. The prepared
microcapsules were analyzed by Scanning Electron Microscopy (SEM). The stability of the microcapsules, the efficiency of the
microencapsulation method and the jojoba oil content determination were also investigated. This method of application improved
the fabric surface and permitted in maximum the preserving of initial characteristics of knit, such as touch, flexibility
and lightness. 相似文献
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为了探讨不同基因型、不同外植体和不同培养条件对小麦愈伤组织诱导、分化及再生的影响,以小偃22、西农1376、西农1012和西农1013为材料,对各小麦品种(系)的幼穗、花药、幼胚和成熟胚组织的培养特性及影响因素进行了研究.结果表明,愈伤组织的诱导和再生与外植体类型、基因型和培养条件密切相关.以幼穗和花药为材料,西农1376平均出愈率均最高,达98.97％和3.85％,成苗率和花培效率均值亦最高,达12.83％和2.39％;以幼胚为材料,西农1376平均出愈率也最高,达99.27％,西农1012平均成苗率最高,达51.03％;以成熟胚为材料,小偃22平均出愈率最高,达93.22％,西农1376平均成苗率达19.78％.离体培养条件下,不同小麦品种(系)的4种外植体愈伤诱导和分化率与2,4-D浓度密切相关,1.0～3.0 mg·L-1 2,4D浓度能较好地诱导4种离体组织愈伤的产生及分化.不同基因型和同一基因型的不同外植体在2,4-D最佳诱导浓度上也有差异.供试小麦4种离体组织培养再生特性比较表明,离体幼胚培养再生效率最高,成熟胚次之,幼穗最差. 相似文献
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《Industrial Crops and Products》1999,9(2):115-123
The oxidative stability indices (OSI) of several vegetable oils were determined at 110°C. Meadowfoam oil, Limnanthes alba, was found to be the most stable oil with an OSI time of 67.3 h for refined oil and 246.9 h for crude oil. Other oils with good oxidative stabilities were refined high oleic sunflower and crude jojoba oil (Simmondsia chinensis) with OSI times of 49.8 and 34.5 h respectively. The unusually high OSI time of crude meadowfoam oil could not be attributed to its tocopherol content since refining did not significantly alter the tocopherol content, but significantly reduced the stability. A relationship of iodine value to antioxidant was developed for vegetable oils; however, this linear relationship did not account for the unusually high oxidative stability of meadowfoam oil. Binary mixtures of vegetable oils were also examined for enhanced oxidative stability. Small amounts of crude meadowfoam oil gave enhanced oxidative stability in mixtures with jojoba, triolein and castor oils. Triolein/crude meadowfoam oil mixtures showed the most dramatic improvements in OSI time with a 5% (w/w) addition of crude meadowfoam causing a 21-fold increase in the OSI time. Meadowfoam mixtures with jojoba improved the OSI time of jojoba from 31.1 to 52.7 h, when 10% crude meadowfoam oil was added. A study of oxidative stability with respect to olefin position showed that the Δ5 double bond was the most stable by more than an order of magnitude. The oxidative stability of meadowfoam FAMEs at 90°C gave an OSI time of 4.9 h which was the same as methyl erucate and methyl petroselenate, but less than methyl oleate which had an OSI time of 14.8 h. However, when methyl 5-eicosenoate was isolated in high purity (monoene >96%) and tested, it gave an OSI time of 69.4 h. 相似文献
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In the present study, various explants of Murraya paniculata (Jack) Linn., such as cotyledons, shoots and young stems were cultured on MS medium supplemented with various concentrations of Benzyl Amino Purine (BAP) under 25 +/- 1 degree C with 16 h light and 8 h dark and also 8 h light and 16 h dark to obtain complete plant regeneration. In vitro flowering was observed from shoot explants cultured on MS supplemented with 0.5-2.0 mg L(-1) Naphthalene Acetic Acid (NAA) and also on MS basal medium under similar conditions. The leaves and flowers obtained from both in vivo and in vitro conditions were examined and compared. Morphological studies such as leaf clearing, epidermal peeling were studied using light and scanning electron microscope. Macromorphological studies of the flowers produced from in vivo and in vitro conditions were also examined. Morphologically, there were no differences between in vivo and in vitro flowers except the flowers produced from tissue culture systems were smaller in size with protruding stigmas. Differences were also found in the number of layers of palisade cells and the presence or absence of epicuticle layer of the leaves. Leaves produced from tissue culture system were smaller in size with membranous texture. Stomata were present only on the abaxial surfaces of both in vivo and in vitro leaves but the stomata were raised above the epidermis in the latter. 相似文献
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植物离体培养物中鱼藤酮的分离与HPLC测定 总被引：3，自引：0，他引：3
毛鱼藤（Ｄｅｒｒｉｓ ｅｌｌｉｐｔｉｃａ （Ｒｏｘｂ．）Ｂｅｎｔｈ．）和西非灰毛豆（Ｔｅｐｈｒｏｓｉａ ｖｏｇｅｌｉｉ Ｈｏｏｋ．ｆ．）离体培养物经９：１ＣＨＣｌ３／ＭｅＯＨ溶剂提取,Ｃ－１８柱层析分离后,在室温条件下以６６：３４ＭｅＯＨ／Ｈ２Ｏ或４２：５８ＣＨ３ＣＮ／Ｈ２Ｏ为流动相,支样品进行反相柱ＨＰＬＣ分析。结果表明,毛鱼藤和西百灰毛豆的愈伤组织均能合成鱼藤酮,但含量相当低,分别为１７．４μｇ／ｇ,１．８μｇ／ｇ干重。经诱导生根分化的毛鱼藤愈伤组织鱼藤酮含量达１３６．２μｇ／ｇ干重,为愈伤组织含量的７．８倍。西非灰毛豆悬浮培养物的鱼藤酮含量虽比愈伤组织有很大提高,但还不及毛鱼藤愈伤组织含量。结果表明,植物的基因类型在次生代谢上起重要作用,同时离体组织或细胞的分化有助于鱼藤酮的生物合成。 相似文献