共查询到20条相似文献，搜索用时 203 毫秒
1.
《Medicinal Plant》2014,(6)
[Objective]To research the apoptosis of hepatoma cells HepG-2 induced by shikimic acid from FRUCTUS ANISI STELLATI and its mechanism. [Methods]Hepatoma cells HepG-2 at logarithmic phase were randomly divided into drug group and control group. Shikimic acids from FRUCTUS ANISI STELLATI at the concentrations of 0. 125,0. 5 and 1 g /L were used. Inhibitory effects of shikimic acids on HepG-2 cell proliferation were detected by MTT method. Effects of shikimic acids at the concentrations of 0. 125,0. 5 and 1 g /L on the cell cycle of hepatoma cells HepG were researched by Flow Cytometer. Expression of Bcl-2 and Bax proteins were detected by immunocytochemistry detection. [Results]Results of MTT test showed that shikimic acid(0. 125,0. 5 and 1 g /L) had significant inhibitory effects on the proliferation of in vitro human liver cancer HepG-2 in a time-and dose-dependent relationship(P 0. 05). Shikimic acid blocked the hepatoma cells HepG-2 at G1 phase,so that the cells could not enter into S phase for DNA synthesis,which restricted the cell proliferation. Results of immunocytochemistry detection showed that protein expression of Bcl-2 decreased significantly,while Bax expression enhanced significantly,so that the proportion of Bcl-2 to Bax reduced. [Conclusions]shikimic acid blocked the hepatoma cells HepG-2 at G1 phase,and had significant inhibitory effects on the proliferation of human hepatoma cell through reducing the proportion of Bcl-2 to Bax. 相似文献
2.
为建立西兰花毛状根高增殖能力悬浮培养体系,实验对通过发根农杆菌ATCC15834浸染西兰花叶片获得的毛状根进行PCR检测,高增殖液体悬浮培养体系条件的优化,毛状根生长动态与萝卜硫素合成动力学研究。实验结果表明,诱导出的毛状根整合了发根农杆菌ATCC15834的rol B基因。毛状根液体悬浮培养的最佳条件为:接种量为1mg/mL、转速为110r/min、培养基体积100mL、培养温度为25℃,增殖倍数达26.47倍。毛状根悬浮培养生长分为迟滞期、分化期、增殖期、静止期、衰亡期五个时期,萝卜硫素的合成于培养的静止期,并向培养基中少量释放,且最佳收获时间为接种后的第21天。这一研究结果可为次生代谢产物萝卜硫素的生产提供一定的理论基础。 相似文献
3.
以高粱无菌苗幼叶为试验材料,对颗粒状胚性愈伤组织的获得、细胞悬浮系的建立及影响悬浮细胞生长的主要因素进行了研究。结果表明：幼叶在MS（Murashige and Skoog）+2mg/L 2,4-D培养基上诱导出的愈伤组织,经2~3次继代筛选后获得了浅黄色、颗粒状胚性愈伤组织;胚性愈伤组织接种于液体培养基中,于25±1℃、黑暗条件下,经45~60d的悬浮震荡培养建立了高质量的细胞悬浮系,细胞生长曲线呈“S”型,细胞密度可达6.45×10 5~5.08×10 6个/mL以上,活细胞率可达72.76%,近圆细胞率可达87.50%;培养基的基本成分、激素种类和水平对悬浮细胞生长状态有很大的影响,相比1/2MS培养基,MS培养基为适宜的培养基,2,4-D对保持细胞系的稳定增殖至关重要,适宜的浓度为1mg/L;培养液中加入0.5mg/L KT或6-BA会造成悬浮液褐化,影响悬浮细胞的生长;最适宜的细胞悬浮培养基为L2培养基（MS+1mg/L 2,4-D,30g/L蔗糖,pH5.8）,震荡培养转速为110~120r/min,继代周期为7d,新旧培养基的接种比例为2∶1。 相似文献
4.
甘薯胚性细胞悬浮培养系的建立 总被引：5，自引：0，他引：5
地甘薯胚性细胞悬浮增减系的进行了研究。将１２个基因的长约０．５ｍｍ的茎尖培养在含有０．２ｍｇ／Ｌ或２．０ｍｇ／Ｌ２，４－Ｄ的ＭＳ培养基上，形成了胚性愈伤组织。胚性愈伤组织的形成率因基因型和２，４－Ｄ深度不同而很大差异，为０－７５．７％。一方面，将胚性愈伤组织继续增减在含有２，４－Ｄ的ＭＳ培养基上，它们形成了处于各发育时期的体细胞胚。将具有体细胞胚的胚性愈伤组织转移到ＭＳ基本培养基上，体细胞胚发育成 相似文献
5.
多花黄精组织培养快繁技术的研究 总被引：3，自引：0，他引：3
[目的]:建立多花黄精组织培养快速繁殖体系。[方法]:以多花黄精带芽根茎为外植体,消毒后将其置于富含不同激素配比的培养基中培养,筛选各阶段合适的培养基。[结果]:在附加有2,4-D的诱导培养基上出现不定芽,增殖培养以MS+6-BA4.0 mg/L+2,4-D0.2 mg/L为好,增殖倍数可达10倍。在增殖培养基中加入GA3有利于壮苗。6-BA、2,4-D、GA3组合更加有利于形成粗壮无根苗。培养基1/2 MS+IBA0.7 mg/L最适合用于诱导黄精不定芽生根,生根率可达95%。[结论]:2,4-D比NAA更有利于多花黄精的不定芽诱导。该繁殖体系可在短时间内提供大量黄精种苗。 相似文献
6.
S. E. Kintzios M. Barberaki G. Aivalak.is J. Drossopoulos C. D. Holevas 《Plant Breeding》1997,116(2):113-118
Callus cultures were initiated from mature excised embryos of winter wheat on Murashige and Skoog (MS) medium supplemented with either 0, 3, 6 or 9g/l NaCl and various combinations of growth regulators. Callus induction rates were virtually 100% on every medium used, while the growth of calli during the induction phase was retarded at higher NaCl concentrations. Application of NaCl and auxin at high concentrations during the induction and proliferation phases strongly inhibited shoot induction. In addition, a competition between root induction and shoot induction was clearly observed. Conversely, rhizogenesis was not significantly decreased under saline conditions and was actually favoured at high NaCl (≥6 g/1) concentrations, especially when 2,4-D was used as the only auxin for callus induction. Roots induced at lower NaCl levels were significantly longer, thinner, lighter-coloured and had fewer hairs than roots induced on 6 or 9 g/1 NaCl. Roots induced on NaCl-free medium also had fewer but larger cortex parenchymatic cell layers and a wider stele compared with roots induced on the highest NaCl level. The best plant regeneration (simultaneous root and shoot formation) rates were observed when calli were induced on 2 mg/1 2,4-D and subcultured on 1 mg/1 NAA and 3 g/1 NaCl. 相似文献
7.
本研究旨在建立西番莲细胞悬浮培养体系,并优化悬浮系培养条件,为西番莲遗传工程提供基础。本研究以西番莲无菌苗为材料,研究西番莲愈伤组织的诱导和建立细胞悬浮系,并结合单因素试验与正交试验优化悬浮系培养条件。结果表明,西番莲愈伤诱导的外植体最佳选择是幼苗胚轴,愈伤诱导激素选择2 mg/L 2,4-D为宜,出愈率达83.3%。以疏松健康的愈伤组织培养细胞悬浮系,经单因素试验和正交试验,细胞悬浮系培养最佳培养培养基为MS培养基+2 mg/L 2,4-D+0.2 g/L Glutamine+30 g/L蔗糖,培养基pH 6.1,起始细胞用量为6.0 mg/mL,摇床转速为100 r/min,28℃暗培养,此条件下建立的细胞悬浮系均匀一致,细胞生长旺盛,西番莲悬浮细胞生长曲线呈“S”型,继代最佳时期为12~18天。本研究为后续利用细胞悬浮系建立西番莲高效细胞工程及遗传转化技术体系提供良好的材料和技术基础,对加强西番莲种业发展具重要意义。 相似文献
8.
匍枝筋骨草悬浮培养生产蜕皮甾酮的研究 总被引：2，自引：1，他引：1
为得到生长迅速、生物量大、蜕皮甾酮含量高的匍枝筋骨草(Ajuga lobata D.Don)细胞系,从固体培养基选择、激素种类筛选出筋骨草颗粒状的胚性愈伤组织,再从培养基类型、激素配比和接种量3个方面优化悬浮培养体系。试验结果表明：添加0.4 mg/L 2,4-D的MS固体培养基可以诱导匍枝筋骨草组培苗的根段产生松散的颗粒状胚性愈伤。比较细胞系在WPM、MS、1/2MS 3种液体培养基中的生长状况,确定MS为最佳的悬浮培养基。2,4-D对细胞生长有显著影响,细胞生物量可达到添加NAA生物量的1.3倍;其与6-BA配比对筋骨草细胞的影响研究结果表明,随着6-BA的浓度增加,蜕皮甾酮的含量随之增加,其中,添加0.5 mg/L 6-BA的筋骨草细胞中蜕皮甾酮含量与不添加6-BA的对照相比,差异显著(P<0.05);添加1 mg/L 6-BA积累的蜕皮甾酮与对照组差异极显著(P<0.01)。因此,0.4 mg/L 2,4-D+0.5 mg/L 6-BA为悬浮培养的最优激素配比,培养7天后细胞生物量净增长生物量可达到(4.3652±1.0739) g,蜕皮甾酮含量可达到(4.5692±0.2044) mg/g,所形成的细胞系更均一且繁殖速率最快。接种量为15%时,细胞长势好,鲜重增殖倍数达3.45,第7天细胞生长速率达1.109 g/d,为最适的接种量。试验建立了生长迅速、悬浮液均一的匍枝筋骨草细胞悬浮培养体系,适于蜕皮甾酮的大量生产。 相似文献
9.
Summary Shoot development through morphological transformation in spikelets occurred after segments of young unemerged orchardgrass (Dactylis glomerata L.) inflorescences were cultured on Linsmaier and Skoog's RM medium supplemented with 0, 4.52×10-4, 4.52×10-3 and 2.26×10-2 mM 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthaleneacetic acid (NAA). Effects on shoot formation were better with 2,4-D than NAA in all concentrations tested. The callus initiated from the primary culture on high 2,4-D medium was reproducible, but no evidence of shoot proliferation was noted. The shoots developed into healthy plantlets after being reared on RM medium not supplemented with hormones.Contribution from South Dakota Agricultural Experiment Station Journal Article No. 1741. 相似文献
10.
掌叶半夏细胞悬浮培养及单细胞培养再生植株 总被引：6，自引：0，他引：6
掌叶半夏成熟胚在含２，４－Ｄ２．０ｍｇ／Ｌ，ＢＡ０．５ｍｇ／Ｌ的ＭＳ培养基上可诱导形成颗粒状胚性愈伤组织。用附加２，４－Ｄ ２．０ｍｇ／Ｌ，ＢＡ０．１ ｍｇ／Ｌ，ＣＨ３００ｍｇ／Ｌ的ＭＳ液体培养基对胚性愈伤组织进行悬浮振荡培养，经３￣４次继代培养即可得到悬浮的单细胞，其细胞多为圆形或椭圆形，具有较强的分裂能力。经测定，悬浮培养过程中细胞生长曲线呈“Ｓ”型，培养２０天时细胞数量和鲜重达到最大值；随着 相似文献
11.
During the long process of evolution, plants have adapted to their environments through the biosynthesis of secondary metabolites (SM) using the acetate-malonate pathway, mevalonic acids pathway, or shikimic acid pathway, leading to resistance to different pests. As a major cash crop, cotton can produce many secondary metabolites with insecticidal activity, and show self-defense mechanisms under biotic stress conditions. These characteristics of cotton ensure reduced pest incidence and maintenance of ecological balance. This paper discusses the types of SMs, their synthesis, and insect resistance of cotton secondary metabolites in relation to their defense function. We propose possible pest-control strategies using secondary metabolites in cotton. 相似文献
12.
NaCl-resistant (selected at 300 mM NaCl) and non-selected cell lines of Vigna radiata were compared for growth, various ions and metabolites at weekly intervals up to 28 days of culture in the presence or absence of NaCl (0–300 mM). The sale-resistant line grew significantly better than the non-selected line at NaCl concentrations higher than 50 mM. The resistant line accumulated significantly more Na+ and maintained higher levels of K+ under NaCl stress than the non-selected line. Both lines accumulated various metabolites, e.g. protein, proline and soluble amino-nitrogen under stress, but this accumulation was greater in the non-selected line than in the resistant one. On the other hand, total content of reducing sugars and sucrose (soluble sugars) was significantly higher in the latter than the former. Moreover, the concentration of soluble sugars in resistant calli was much higher than the other intracellular metabolites. Thus, sugars and ions are likely the major osmoregulants in the resistant line of V. radiata under salt stress. 相似文献
13.
14.
细胞悬浮系的中短期保存广泛需要。本文应用中花15细胞悬浮系,比较了AA、N6、MS三种固体培养基对水稻细胞悬浮系的繁殖保存效果,结果表明,AA固体培养基保存效果最好。细胞系可以在AA0培养基上4个月、在AA0.5培养基上6个月(中间45 d左右继代一次)连续保存后仍然保持其可重悬性。通过比较AA固体培养基繁殖保存、冷冻保存、连续悬浮培养3种保存方法,表明AA固体培养基繁殖保存细胞系2~9个月内,既可保持细胞的可悬浮性,又对细胞系的POD、SOD活性和植株再生率影响较小,是一种理想的细胞悬浮系中短期保存方法。 相似文献
15.
16.
【研究目的】研究了不同剂量甜菜碱对三黄鸡胚胎原代成纤维细胞微核和增殖的影响,在细胞水平上揭示甜菜碱对三黄鸡生长影响的机理,为甜菜碱在动物生产中的应用提供依据。【方法】体外分离培养三黄鸡胚胎成纤维细胞,在培养液中添加不同剂量甜菜碱,观察细胞生长及分裂。【结果】①在基础培养液（DMEM+15%NBS）中添加10mM,20mM甜菜碱对三黄鸡胚胎原代成纤维细胞微核率无显著影响;添加30mM,40mM,50mM甜菜碱显著提高三黄鸡胚胎原代成纤维细胞的微核率。②在基础培养液（DMEM+15%NBS）中添加10mM、20mM甜菜碱能促进三黄鸡胚胎原代成纤维细胞的生长和增殖,以在培养液中添加20mM甜菜碱时三黄鸡原代成纤维细胞增殖速度最快。添加50mM甜菜碱时抑制了三黄鸡胚胎原代成纤维细胞的生长和增殖。【结论】低剂量甜菜碱可促进三黄鸡胚胎原代成纤维细胞的生长和增殖,高剂量甜菜碱对细胞分裂构成危害。 相似文献
17.
Summary The feasibility of developing an in vitro technique for screening drought-tolerant coconut germplasm has been investigated. Embryos excised from mature nuts of Sri Lanka-tall coconut were cultured as described previously. Water-stress in the culture system was progressively increased with each passage, by incorporating polyethylene glycol (PEG-6000), mannitol and sodium chloride into the culture medium. PEG and mannitol were observed to be growth inhibitory in action event at low concentrations and these two compounds were abandoned. In NaCl-stressed media, about 21% of randomly selected Sri Lanka-tall embryos died before reaching the 170 mM NaCl. About 78% survived 170 mM NaCl and only 12.6% were able to resist 320 mM NaCl. When zygotic embryos derived from two known drought-susceptible cultivars of coconut, CRIC-65 and Dwarf (from pumila) were tested using the same technique, 29% and 73% of embryos respectively died due to stress damage caused by 170 mM NaCl and none of either cultivar survived a salt concentration above 230 mM.However, embryos originated from two putative drought-tolerant cultivars showed a higher survival rate when subjected to salt stress. At 170 mM NaCl, all the embryos had developed into seedlings. In fact, percent germination of embryos was somewhat higher in 170 mM NaCl than in the control, that was devoid of NaCl. However, percent survivors gradually dropped with increase in salt concentration and about 18% survived the 330 mM NaCl. The technique seems to have great potential in screening drought-tolerant coconut germplasm. 相似文献
18.
19.
Somatic embryos differentiated directly on the rachis of immature inflorescences of Paspalum scrobiculatum L. cv. PSC 1 on culture to MS or N6 medium supplemented with different concentrations (4.5–22.5 μM) of 2,4-dichlorophenoxyacetic acid (2,4-D). Direct embryogenesis
on the rachis of inflorescence explants forms the first instance in graminaceous plants. Highest frequency of direct embryogenesis
(34%and 30% cultures, respectively) was possible on N6 medium supplemented with 4.5 μM of 2,4-D and MS medium fortified with9.0 μM of 2,4-D. Other tissues of the explant, floral-primordia,
only after an initial phase of callusing differentiated into somatic embryos; indirect embryogenesis. Somatic embryogenesis,
direct as well as indirect, was resolved by scanning electron microscopy. The somatic embryos germinated and developed into
plantlets on regeneration medium. Interestingly, one week incubation of somatic embryos on activated charcoal (0.5%) fortified
basal medium, supported high potential for ‘germination’ on transfer to charcoal-free basal medium. This beneficial effect
of activated charcoal on regeneration of somatic embryos into plantlets is the first record in the Gramineae.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
20.
连翘酯苷对内毒素作用下RAW264.7细胞功能的影响 总被引：1，自引：0，他引：1
为探讨连翘酯苷(FS)对内毒素(LPS)作用下RAW264.7细胞增殖、分泌NO和TNF-α、吞噬功能的影响。收集处于对数生长期细胞,用含10%胎牛血清的RPMI1640培养细胞,在培养液中分别加入脂多糖(LPS)以及不同浓度40、80、160 μg/mL的FS共培养。采用MTT法检测细胞增殖,Griess和ELISA法分别检测RAW 264.7细胞分泌NO和TNF-α量,染色法检测细胞吞噬能力。结果表明：低、中剂量FS可显著促进细胞增殖,而中和高剂量FS可缓解LPS对细胞的刺激作用;与LPS组比较,FS对LPS刺激的RAW 264.7细胞分泌NO影响不明显,但中、高剂量FS明显促进RAW 264.7细胞分泌;LPS与FS均能提高巨噬细胞吞噬鸡红细胞能力。FS对RAW264.7细胞增殖、分泌NO和TNF-α以及吞噬功能都有影响,结果提示这可能是连翘酯苷调节细胞免疫功能的机制之一。 相似文献