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《园艺学报》2007,34(2):310-310,402,460,500,524
CNKI中国引文数据库《园艺学报》高被引频次论文(截至2007年4月)序号被引文献题名被引文献作者被引文献来源被引频次1蔬菜硝酸盐累积的研究———Ⅰ.不同蔬菜硝酸盐沈明珠,翟宝杰,东惠茹,李俊国园艺学报/1982/04298和亚硝酸盐含量评价2温室土壤次生盐渍化的形成和治理途径研究童有为,陈淡飞园艺学报/1991/021473氮磷钾对叶菜硝酸盐积累和硝酸还原酶、过氧高祖明,张耀栋,张道勇,史瑞和,章满芬园艺学报/1989/04114化物酶活性的影响4黄瓜种质资源遗传亲缘关系的RAPD分析张海英,王永健,许勇,欧阳新星,康国斌园艺学报/1998/041005切花保鲜剂… 相似文献
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《园艺学报》是中国园艺学会和中国农业科学院蔬菜花卉研究所主办的学术期刊,创刊于1962年,刊载有关果树、蔬菜、观赏植物、茶及药用植物等方面的学术论文、研究报告、专题文献综述、问题与讨论、新技术新品种以及园艺研究动态与信息等,适合园艺科研人员、大专院校师生及农业技术推广部门专业技术人员阅读参考。《园艺学报》是中文核心期刊,被英国《CAB文摘数据库》、美国CA化学文摘、日本CBST科学技术文献速 相似文献
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XING Ling-xiao ZHANG Xiang-hong LI Yue-hong YAN Xia WANG Jun-ling WANG Feng-rong 《园艺学报》2004,20(3):306-310
AIM: To explore the effects of sterigmatocystin (ST) on IL-2 and IFN-γ expression and secretion in murine spleen cells in vitro. METHODS: The secretion and expression of IL-2 and IFN-γ in murine spleen cells after ST pretreatment at five different dosages(0.125 mg/L,0.25 mg/L,0.5mg/L,1 mg/L,2 mg/L) were studied with ELISA and semi-quantitative RT-PCR method, respectively. RESULTS: Pretreatment of murine spleen cells in vitro with ST at five different dosages affected the IL-2 and IFN-γ secretion at protein level and expression at mRNA level of the treated cells. The effects varied dependently to the ST dosage. At relatively lower dosages, ST induced the expression of IL-2 and IFN-γ in murine spleen cells, while at relatively higher dosages, inhibitory effects were found, with the most significant inhibitory effects seen in ST 1 mg/L group. CONCLUSION:ST affected the se-cretion and expression of IL-2 and IFN-γ in treated murine spleen cells.At relatively lower dosage, ST induced IL-2 and IFN-γ secretion and expression, while at relatively higher dosages，inhibitory effects appeared. 相似文献
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AIM: To investigate the effect of homocysteine(Hcy) on secretion and expression of interleukin-6(IL-6), which is a multifunctional proinflammatory cytokine, in cultured rat vascular smooth muscle cells(VSMCs). METHODS: Rat VSMCs were stimulated with Hcy. Cell ELISA was performed to measure the expression of IL-6 protein and semiquantitative RT-PCR was used to dectect the IL-6 mRNA expression. RESULTS: Compared with control, treatment of 0.25 mmol Hcy for 6 h could increase IL-6 production. In addition, Hcy concentration-dependently increased the expression of IL-6 protein in these cells. 0.1 mmol/L, 0.25 mmol/L Hcy increased IL-6 production 1 4-fold and 3 4-fold, respectively Furthermore, RT-PCR analysis demonstrated that homocysteine also enhanced IL-6 mRNA expression in a concentration- and time-dependent manner.CONCLUSION: Homocysteine can induce IL-6 expression in VSMCs and elicit vascular inflammatory response, which may thereby influence the pathogenesis of atherosclerosis. 相似文献
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LI Fei WANG Jing-feng NIE Ru-qiong LUO Nian-sang GENG Deng-feng YUAN Wo-liang XIE Shuang-lun LIN Yong-qing ZHAO Wen-jie 《园艺学报》2008,24(5):909-914
AIM: To investigate the effects of ox-LDL on TLR2 and TLR4 expression and production of TNF-α, IL-10, IL-12, NO and MDA in macrophages and to observe intervention effect of GW1929 in above procedure. METHODS: The mouse peritoneal macrophages were pretreated with ox-LDL (50 mg/L, 100 mg/L) and GW1929 (20 μmol/L) respectively for 24 h. The concentrations of MDA, NO-2/NO-3, TNF-α, IL-10 and IL-12 in the culture fluid were detected. Flow cytometry was used to observe TLR2 and TLR4 expressions after the mouse peritoneal macrophages were pretreated with ox-LDL (50 mg/L) and GW1929 (20 μmol/L) respectively for 6 h, 12 h, and 24 h. RESULTS: The concentrations of MDA, NO-2/NO-3, TNF-α and IL-10 in ox-LDL (50 mg/L, 100 mg/L) group were higher than those in control and GW1929 group obviously, but the concentrations of above index in ox-LDL (50 mg/L, 100 mg/L)+GW1929 group were lower than those in ox-LDL (50 mg/L, 100 mg/L) group apparently. No IL-12 in every group was detected. Expressions of TLR-2 in ox-LDL+GW1929 (6 h, 12 h, 24 h) group were lower than those in ox-LDL (6 h, 12 h, 24 h) group respectively. TLR-4 expressions in ox-LDL+GW1929 (12 h) were lower than those in ox-LDL (12 h) apparently. CONCLUSION: ox-LDL up-regulates TLR2 and TLR4 expressions and promotes the production of ROX, NO, TNF-α and IL-10 in macrophages. GW1929 is capable of inhibiting the above ox-LDL effects. 相似文献
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HUANG Xiang-hua ZHANG Xiang-hong YAN Xia YIN Gui-ran LI Yue-hong TAN Yan-wei WANG Jun-ling WANG Feng-rong 《园艺学报》2002,18(2):169-171
AIM:To explore the putative effects of sterigmatocystin (ST) on human help T lymphocyte(Th1)function. METHODS:The effects of ST on interferon-γ(IFN-γ)secretion of human peripheral blood mononuclear cells(HPBMc) in vitro were determined with ELISA method. RESULTS:The effects of ST on IFN-γ secretion of HPBMc in vitro were closely dependent on ST concentrations. ST at relatively lower concentrations (0.03125-0.12500 mg/L) showed inhibiting effects on IFN-γ secretion. While, stimulating effects could be found when ST concentration was above 0.25mg/L. The highest level was seen in ST 1 mg/L group (P＜0.05)。At concentration ranging from 0.25 mg/L to 1 mg/L, a positive dose- effects correlation was found between ST concentration and IFN-γ secretion (r=0.492, P＜0.01). Time-effects analysis from 1 h to 64 h after ST treatment (1 mg/L)showed that the effects of ST on IFN-γ secretion varied as the changes of treatment times. An inhibiting effect on IFN-γ secretion of HPBMc 4 h and 8 h after ST treatment was found (8 h, P＜0.05). As the treatment time prolonged from 16 h, IFN-γ level gradually increased (32 h, P<0.05). There was a positive correlation between treatment time of ST and IFN-γ level of HPBMc in vitro from 16 h to 64 h after ST treatment (r=0.736, P＜0.01).CONCLUSION:The effects of ST on IFN-γ secretion of HPBMc in vitro closely depended on concentration and treatment time of ST. Generally, inhibiting effects were found at relatively lower ST concentration and shorter treatment period, while stimulation effects could be seen at relatively higher ST concentration and longer ST treatment time period. 相似文献
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JIA Qing-zhe GE Jun-bo LIANG Chun LUO Yu-kun HUANG Dong WANG Ke-qiang CHEN Hao-zhu 《园艺学报》2006,22(1):139-142
AIM: To investigate the effect of advanced glycosylation end products on the expression of receptor for advanced glycosylation end products in human monocyte-derived dendritic cells. METHODS: Monocytes were purified (over 98%) using anti-CD14＋ microbeads. After 8 d culture in RPMI-1640 medium containing rhGM-CSF (100 μg/L) and rhIL-4 (50 μg/L), immature MDCs were derived, then exposed to AGE-BSA (0 or 200 mg/L) for 24 h. Expression of RAGE was semi-quantified by RT-PCR and Western blotting. At the same time, supernatants were collected. IFN-γ and IL-12 were analyzed by ELISA. RESULTS: mRNA and protein of RAGE incubated by 200 mg/L AGE-BSA was higher than that in control at 24 h. Treatment of DCs with AGE-BSA resulted in about two-fold increase in the expression of RAGE (P<0.05). The concentrations of IFN-γ and IL-12 were both significantly higher than that in control (P<0.05). CONCLUSION: AGEs up-regulates the expression of RAGE and induces the secretion of IFN-γ and IL-12 by DCs. These findings may provide insight into the effect of DCs on the processes of atherosclerosis. 相似文献
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AIM:To determine the effect of unfractionated heparin(UFH) on lipopolysaccharide(LPS)-induced expression of interleukin-8(IL-8) and the role of Toll-like receptor 4(TLR4) signaling. METHODS:Human pulmonary microvascular endothelial cells(HPMECs) were either exposed to LPS alone(10 mg/L) or in combination with 100 U/L or 103 U/L UFH. UFH was added to the cells 15 min prior to stimulation with LPS. Those samples not receiving LPS or UFH received an equal volume of phosphate-buffered saline. The concentrations of IL-8 in the cell culture supernatants were detected by ELISA at 2, 6 and 12 h. The mRNA expression of IL-8, CD14 and TLR4 in HPMECs was detected by real-time fluorescence quantitative polymerase chain reaction at 2, 6 and 12 h. RESULTS:Compared with normal control group, the mRNA expression of IL-8 in LPS group was increased, and reached the peak at 6 h. The protein level of IL-8 reached the peak at 12 h. The mRNA expression of TLR4 in LPS group reached the peak at 6 h. They were down-regulated in UFH group. The mRNA expression of CD14 was not detected. CONCLUSION:The expression of IL-8 is obviously increased in LPS-treated HPMECs. UFH might exert its therapeutic effect through TLR4 signaling. 相似文献
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YU Si-yang WANG Yan ZENG Gao-feng LIU Yang XU Jian-qiang ZENG Meng-ya TANG Ye-hua ZENG Zhi-ying SHI Xiao-qiao CHEN Ying ZHAO Guo-jun 《园艺学报》2016,32(5):863-868
AIM: To explore the role of nucleotide-binding oligomerization domain-like receptor protein 1 (NLRP1) inflammasome in atorvastatin-induced reduction of interleukin-1β (IL-1β) and interleukin-18 (IL-18) releases from the THP-1 macrophages. METHODS: Lipopolysaccharide (LPS, 10 μg/L) was used to trigger the secretion of IL-1β and IL-18 in the THP-1 macrophages. The cells were incubated with different concentrations of atorvastatin (1, 10 and 20 μmol/L) for 24 h, or treated with 10 μmol/L atorvastatin for different time (12 h, 24 h and 48 h). NLRP1 siRNA was transfected into the THP-1 cells. The mRNA expression of NLRP1 inflammasome was detected by RT-PCR. The protein expression of NLRP1 inflammasome was determined by Western blot. The secretion of proinflammatory cytokines IL-1β and IL-18 was quantified by ELISA. RESULTS: Atorvastatin inhibited the mRNA and protein expression of NLRP1 inflammasome in the THP-1 macrophages in a dose- and time-dependent manner. Transfection of NLRP1 siRNA significantly decreased the protein expression of NLRP1 and promoted the suppressive effect of atorvastatin on IL-1β and IL-18 secretion in the THP-1 macrophages. CONCLUSION: Atorvastatin inhibits the production of IL-1β and IL-18 in the macrophages through decreasing NLRP1 inflammasome expression, possibly contributing to the anti-inflammatory effect of atorvastatin on atherosclerosis. 相似文献
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AIM: To investigate the effects of atorvastatin on the expression of pregnancy-associated plasma protein A(PAPP-A)induced by TNF-α and IL-1β in endothelial cells. METHODS: The rat aortic endothelial cells were isolated from thoracic aortas and cultured by the tissue explant method. The cells in passage 3-4 were used in the experiment and were randomly divided into 4 groups: blank control group: the cells were treated without any intervention; atorvastatin concentration groups: the cells were incubated with atorvastatin at the concentrations of 0.1, 1 and 10 μmol/L for 24 h; atorvastatin time groups: the cells were incubated with atorvastatin at the concentration of 10 μmol/L for 6 h,12 h and 24 h; atorvastatin+inflammatory factors groups: the cells were pre-incubated with 60 μg/L TNF-α or 20 μg/L IL-1β for 1 h, then different concentrations of atorvastatin (0.1, 1.0, 10 μmol/L) were added for 6 h,12 h and 24 h. MTT reduction assay was used to observe the cell proliferation. The mRNA expression of PAPP-A was detected by RT-PCR. The protein level of PAPP-A in the supernatants of cultured cells was measured by ELISA. RESULTS: Compared with blank control group, no significant change of cell proliferation was observed after the intervention of atorvastatin and TNF-α/IL-1β for 3 h, 6 h, 12 h, 24 h and 48 h, indicating that the drugs had no toxic effects on the cells. No significant difference of PAPP-A expression between atorvastatin groups and blank control groups was found. Compared with TNF-α groups and IL-1β groups, PAPP-A expressions in atorvastatin intervention groups significantly decreased. The protein level of PAPP-A was gradually decreased with the raised concentration of atorvastatin and the prolonged time in a concentration- and time-dependent manner. CONCLUSION: Atorvastatin doesn't influence the PAPP-A expression, but inhibits the expression of PAPP-A activated by inflammatory factors in a concentration- and time-dependent manner in primary cultured rat aortic endothelial cells. 相似文献