共查询到20条相似文献，搜索用时 171 毫秒
1.
为探究儿茶素对高脂饮食小鼠减肥作用及肠道菌群的影响，将小鼠随机分为对照组、模型组、儿茶素组（0.06%儿茶素+高脂饲料），各组连续喂养28周，记录小鼠体质量和摄食量，计算肝脏系数、体脂比和Lee’s指数，检测血脂指标，并采用HE染色观察小鼠肝脏病理变化，实时荧光定量PCR检测小鼠肠道菌群的相对表达量。研究结果显示，与对照组相比，模型组小鼠体质量、肝脏系数、体脂比和Lee’s指数明显增加，血脂指标总胆固醇（TC）、甘油三酯（TG）及低密度脂蛋白（LDL-C）水平明显高于对照组，高密度脂蛋白（HDL-C）水平明显降低（P<0.05）；与模型组相比，儿茶素组小鼠体质量、肝脏系数及Lee’s指数明显降低，血脂指标TG和LDL-C水平明显降低、HDL-C水平显著增加（P<0.05）。肝组织病理结果显示，儿茶素组小鼠与模型组相比能明显改善肝组织病变程度，减少肝损伤；肠道菌群结果显示，儿茶素组小鼠拟杆菌相对表达量明显高于模型组（P<0.05）。研究结果表明，儿茶素可显著减少小鼠摄食量、降低体质量、调节血脂水平、改善肝组织病变和肠道菌群紊乱，对于高脂饮食小鼠具有较好的减肥作用。 相似文献
2.
以相同鲜叶加工而成的红茶及其发花红砖茶为原料,在应用链脲佐菌素腹腔注射并成功建立高血糖模型小鼠方法的基础上,将90只KM雄性小鼠按每组10只,随机分为正常组、模型组、红茶及发花红砖茶高、中、低剂量组和盐酸二甲双胍片组,连续灌喂28天,探讨了红茶及其发花红砖茶对高血糖模型小鼠的降血糖作用。结果表明,与正常组相比,模型组小鼠体重、饮水、饮食、血糖水平均有极显著差异(P<0.01),且血糖水平达到高血糖标准,说明造模成功;与模型组相比,红茶及发花红砖茶高剂量组的肝脏指数显著下降(P<0.05)、胰腺指数显著上升(P<0.05);中、高剂量组的总胆固醇显著下降(P<0.05),过氧化氢酶、超氧化物岐化酶显著上升(P<0.05);各剂量组的血糖、甘油三酯、丙二醛含量水平显著下降(P<0.05),口服耐糖量、谷胱甘肽过氧化物酶活性及胰岛素含量水平则显著上升(P<0.05),除红茶低剂量组外各剂量组肝糖原含量水平均显著上升(P<0.05),同时可修复受损胰岛;与红茶组相比,发花红砖茶高剂量组在改善胰岛素水平、血糖水平、口服耐糖量、丙二醛水平及肝糖原水平上显著优于红茶高剂量组(P<0.05),中、高剂量组在提高过氧化氢酶、超氧化物岐化酶及谷胱甘肽过氧化物酶的酶活性上显著优于红茶中、高剂量组(P<0.05)。由此表明,红茶及其发花红砖茶均具有降低高血糖模型小鼠血糖的作用,且在作用效果上发花红砖茶优于红茶,但其机制有待进一步探究。 相似文献
3.
茶色素对胰岛素抵抗大鼠胰岛素受体的影响 总被引：1，自引：1，他引：0
探讨茶色素对胰岛素抵抗大鼠红细胞胰岛素受体的影响。将50只清洁级SD大鼠随机分成对照组、高脂组和低中高剂量茶色素干预组。高脂组和茶色素干预组以高脂饲料饲喂8周诱发肥胖致胰岛素抵抗模型,茶色素干预组再给予0.1、0.2g/(kgbw·d)和0.4g/(kgbw·d)剂量茶色素灌胃8周,实验结束后采血测定红细胞胰岛素受体水平,同时测定血糖、血胰岛素、血FFA及血痩素。与对照组相比,高脂组空腹胰岛素、痩素和FFA明显升高(P<0.05),而红细胞高亲和力受体数目明显降低(P<0.05),与高脂组相比,中高剂量茶色素干预组空腹胰岛素、痩素和FFA明显降低(P<0.05),而红细胞高亲和力胰岛素受体数目明显升高(P<0.05)。说明茶色素干预可提高胰岛素抵抗大鼠胰岛素高亲和力受体数目,其机制可能与降低痩素和FFA水平间接提高受体数目或直接作用于受体基因调节元件有关 相似文献
4.
[目的]探讨蛹虫草对小鼠体液免疫功能和NK细胞活性的影响。[方法]以83.3、166.7、333.3 mg/kg·BW剂量的蛹虫草给予小鼠灌胃30 d。[结果]灌胃30 d后同对照组比较,各剂量组对小鼠体重、脾/体重、胸腺/体重比值无影响(P>0.05),中、高剂量组能显著升高小鼠血清半数溶血值(P<0.05),中、高剂量组能显著增强抗体生成细胞能力(P<0.05),各剂量组均能增强小鼠NK细胞活性(P<0.05)。[结论]蛹虫草具有增强体液免疫功能和NK细胞活性的作用。 相似文献
5.
普洱茶多糖降血脂功能的量效关系 总被引：10，自引：0，他引：10
研究结果表明,与高脂对照组比较,低剂量茶多糖组(3组)小鼠血清TG、TC、LDL-C、HDL-C水平及AI值没有显著差异。而中、高剂量组(4、5组)小鼠血清TG、TC、LDL-C水平分别下降了40.60%、25.36%、24.38%;40.02%、26.68%、33.59%。同时,HDL-C水平分别提高了73.44%、82.81%。但中、高两个剂量组之间没有明显差异。表明茶多糖抑制高脂饮食小鼠血脂升高可能存在量效关系。 相似文献
6.
《大豆科学》2015,(4)
通过建立大鼠高血脂症动物模型,用不同剂量的自制大豆小肽灌胃大鼠,分别在第20,40,60天时尾静脉采血测定血液生化指标,并在第21天进行游泳负重实验和肝糖原含量测定,探讨了大豆低聚肽在调解血脂、预防动脉粥样硬化、抗疲劳作用。结果表明:灌胃60 d时高剂量组的大鼠的TC、TG、LDL-C、TXA2相对于模型对照组显著降低,分别降低22.97%、8%、15.29%、18.97%,高剂量组大鼠的NO、HDL-C、PGI2相对于模型对照组显著升高,分别升高了43.85%%、20.56%、28.92%。抗疲劳试验中,在灌胃剂量2.0 g·kg-1(体重)时,小鼠负重时间比对照组延长61.49%,小鼠肝糖原含量为20.07 mg·g-1,与对照组相比提高了41.14%。表明大豆小肽具有明显的预防动脉粥样硬化和抗疲劳的功能。 相似文献
7.
8.
目的探讨参红补血颗粒(SBG)对血虚型血管内皮功能障碍(VED)小鼠模型的血管内皮保护作用,阐明其相关作用机制。方法通过腹腔注射环磷酰胺联合乙酰苯肼法建立血虚型VED小鼠模型,随机分为VED组、阳性对照组、SBG高剂量组、SBG中剂量组和SBG低剂量组,以腹腔注射等量生理盐水小鼠作为对照组。观察小鼠脏器指数,外周血象指标,HE染色法观察小鼠胸主动脉组织病理形态学,试剂盒检测血清中一氧化氮(NO)含量和胸主动脉组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性,Western blotting法检测胸主动脉组织中TLR4、MyD88、NF-κBp65、ICAM-1蛋白表达量。结果与VED组比较,阳性对照组及SBG高、中、低剂量组小鼠胸腺指数显著升高,脾脏指数明显降低(P<0.01,P<0.05),RBC、HGB、HCT、WBC、PLT水平显著升高(P<0.01,P<0.05),GSH-Px和SOD水平显著升高(P<0.01,P<0.05),TLR4、MyD88、NF-κBp65、ICAM-1明显降低(P<0.01,P<0.05)。结论SBG可以通过抑制TLR4信号通路,降低炎症因子水平,减轻氧化应激损伤,达到保护气滞血瘀型VED小鼠血管内皮的作用。 相似文献
9.
以相同品种的鲜叶加工而成的红茶及其发花红砖茶为原料,在应用氧嗪酸钾成功建立高尿酸血症模型小鼠方法的基础上,将90只KM雄性小鼠按每组10只随机分为正常组、模型组、红茶及发花红砖茶高、中、低剂量组和别嘌呤醇组,连续灌喂14 d,探讨红茶及其发花红砖茶对高尿酸血症模型小鼠的降尿酸作用。结果表明,与模型组相比,给茶各剂量组的体质量、肾脏指数、血尿酸均显著降低(P<0.05),其中发花红砖茶中、高剂量组在降低血尿酸上达到极显著水平(P<0.01);两种茶各剂量组的血尿素氮、血肌酐也均极显著降低(P<0.01),且中、高剂量组的尿尿素氮极显著升高(P<0.01),高剂量组的尿尿酸极显著升高(P<0.01);除红茶低剂量组尿肌酐水平显著升高(P<0.05)之外,其他给茶组的尿肌酐均达到极显著升高(P<0.01);除发花红砖茶中剂量组显著降低黄嘌呤氧化酶和腺苷脱氨酶活性(P<0.05)之外,红茶中剂量组及两种茶高剂量组均能极显著降低这两种酶的活性(P<0.01)。结果表明,红茶及发花红砖茶均可减少尿酸的产生,且发花红砖茶高剂量组在降低血尿酸与升高尿尿酸、尿尿素氮、尿肌酐方面优于红茶,其机制有待进一步探究。 相似文献
10.
为了探讨陈年六堡茶的调脂保肝功效,比较分析了不同年代六堡茶的主要化学物质的差异,再以高脂高糖饮食建立高脂血症小鼠模型,并用选定的陈年六堡茶水提物灌胃小鼠,观察小鼠相关生理生化指标及组织形态的变化。结果表明,陈化15年茶样被选为陈年六堡茶的代表,用于后续试验。与模型组相比,陈年六堡茶尤其中高剂量条件下能有效减轻高脂血症小鼠体重、肝重和降低肝系数,显著提高高密度脂蛋白-胆固醇(HDL-C)含量,降低血清中总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白-胆固醇(LDL-C)含量,降低谷丙转氨酶(ALT)和谷草转氨酶(AST)活力,改善大鼠肝脏氧化应激状态,具有统计学意义(P0.05或P0.01)。肝脏和脂肪组织病理切片显示,陈年六堡茶能明显减少肝细胞中脂滴的形成和抑制脂肪细胞膨大,呈剂量依赖关系。综上表明,陈年六堡茶在正常剂量条件下能有效改善因高脂高糖饮食所致的小鼠脂质代谢紊乱和肝脏损伤。 相似文献
11.
This study was designed to investigate the effects of long-term feeding of chitosan on plasma glucose and lipids in rats fed a high-fructose (HF) diet (63.1%). Male Sprague-Dawley rats aged seven weeks were used as experimental animals. Rats were divided into three groups: (1) normal group (normal); (2) HF group; (3) chitosan + HF group (HF + C). The rats were fed the experimental diets and drinking water ad libitum for 21 weeks. The results showed that chitosan (average molecular weight was about 3.8 × 105 Dalton and degree of deacetylation was about 89.8%) significantly decreased body weight, paraepididymal fat mass, and retroperitoneal fat mass weight, but elevated the lipolysis rate in retroperitoneal fats of HF diet-fed rats. Supplementation of chitosan causes a decrease in plasma insulin, tumor necrosis factor (TNF)-α, Interleukin (IL)-6, and leptin, and an increase in plasma adiponectin. The HF diet increased hepatic lipids. However, intake of chitosan reduced the accumulation of hepatic lipids, including total cholesterol (TC) and triglyceride (TG) contents. In addition, chitosan elevated the excretion of fecal lipids in HF diet-fed rats. Furthermore, chitosan significantly decreased plasma TC, low-density lipoprotein cholesterol (LDL-C), very-low-density lipoprotein cholesterol (VLDL-C), the TC/high-density lipoprotein cholesterol (HDL-C) ratio, and increased the HDL-C/(LDL-C + VLDL-C) ratio, but elevated the plasma TG and free fatty acids concentrations in HF diet-fed rats. Plasma angiopoietin-like 4 (ANGPTL4) protein expression was not affected by the HF diet, but it was significantly increased in chitosan-supplemented, HF-diet-fed rats. The high-fructose diet induced an increase in plasma glucose and impaired glucose tolerance, but chitosan supplementation decreased plasma glucose and improved impairment of glucose tolerance and insulin tolerance. Taken together, these results indicate that supplementation with chitosan can improve the impairment of glucose and lipid metabolism in a HF-diet-fed rat model. 相似文献
12.
红茶对高脂饮食小鼠血脂的调节作用研究 总被引：1，自引：0，他引：1
采用脂代谢紊乱模型法—预防性给予不同地区不同剂量的红茶（剂量为成人每日饮用量的5倍、10倍和20倍3种）,观察小鼠体重、血脂、相关酶及肝脏形态的变化。结果表明：与高脂模型对照组相比,红茶剂量组能极显著（P<0.01）降低血清总胆固醇（TC）、低密度脂蛋白（LDL-C）、肝脏丙二醛（MDA）、肝脏指数;极显著（P<0.01）升高血清载脂蛋白A1（apoA1）、脂蛋白脂酶（LPL）、肝脂酶（HL）、总脂酶、肝脏超氧化物歧化酶（SOD）及谷胱甘肽过氧化物酶（GPX）,血清高密度脂蛋白（HDL-C）有所上升,小鼠体重也得到了一定的抑制,肝脏病变程度得到了相应的减轻,说明红茶具有一定的调节血脂及减小肝脏因高脂饮食所带来的损伤。 相似文献
13.
高油酸花生对大白鼠血脂水平影响的研究 总被引：1，自引：0，他引：1
目的：观察高油酸花生在高血脂症形成过程中对大白鼠血脂水平的影响。方法：选取50只Wistar大白鼠，随机分为5组，每组10只，高油酸花生分为5％和10％两个剂量组，普通花生设10％一个剂量组，另设一个高脂饲料组及一个基础饲料组；除基础饲料组外，其他4个组喂含有猪油的高脂饲料一个月，建立高脂模型。模型建好后，分别用10％普通花生饲料、5％高油酸花生饲料和10％高油酸花生饲料喂养一个月后，取尾血，测血清TC、TG、HDL-C、LDL-C四项指标。结果：高油酸花生饲料显示出能降低大白鼠血清中的TC、TG含量（P〈0．01）。 相似文献
14.
Indirani Mani Uliyar V Mani 《Plant foods for human nutrition (Dordrecht, Netherlands)》1987,37(2):161-168
Wheat bran supplementation (11 gm per day) was carried out for a period of two months in 30 maturity onset diebetics. The efficacy of bran supplementation was monitored by measuring the fasting and 2 h post prandial blood sugar levels, glycosylated serum protein levels, glycosylated albumin levels and serum lipid and lipoprotein cholesterol levels. The results indicated a transient reduction in fasting and 2 h post prandial blood sugar levels after one month's bran therapy. No appreciable changes in the serum glycosylated protein levels, glycosylated albumin levels, serum lipids and lipoprotein cholesterol levels were observed during the period of supplementation.Abbreviations TC
total cholesterol
- HDL-C
high density lipoprotein cholesterol
- LDL-C
low density lipoprotein cholesterol
- VLDL-C
Very low density lipoprotein cholesterol
- TG
triglycerides
- PL
phospholipids
- FFA
free fatty acids
- G-Alb
glycosylated albumin
- GSP
glycosylated serum proteins
- NIDDM
Non Insulin Dependent Diabetes Melitus 相似文献
15.
Valcheva-Kuzmanova S Kuzmanov K Mihova V Krasnaliev I Borisova P Belcheva A 《Plant foods for human nutrition (Dordrecht, Netherlands)》2007,62(1):19-24
Aronia melanocrpa fruit juice (AMFJ) used in our experiment was very rich in phenolic substances (709.3 mg gallic acid equivalents/100 ml juice).
Anthocyanins (106.8 mg cyanidin-3-glucoside equivalents/100 ml juice) were the main flavonoid group. The aim of this study
was to assess the influence of AMFJ on plasma lipids and lipoprotein profile, and histopathology of liver and aorta in rats
with dietary-induced hyperlipidemia. AMFJ was administered by gavage for 30 days at doses of 5, 10 and 20 ml/kg body weight
to rats fed a standard diet (SD) or a 4% cholesterol-containing diet (4% ChD). The 4% ChD caused a significant elevation of
plasma total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and triglycerides (TG). AMFJ did not significantly
influence plasma lipids in rats fed the SD and significantly hindered the elevation of plasma TC, LDL-C and TG in rats fed
the 4% ChD. High-density lipoprotein cholesterol (HDL-C) levels were not significantly influenced either by the 4% ChD or
by AMFJ. Neither the cholesterol feeding, nor AMFJ treatment induced any histopathological changes in rat liver and aorta.
In conclusion, AMFJ showed an antihyperlipidemic effect in rats with hyperlipidemia and could be valuable in reducing this
factor of cardiovascular risk. 相似文献
16.
为探究L-茶氨酸（L-theanine,LTA）对来曲唑诱导的多囊卵巢综合征（Polycystic ovarian syndrome,PCOS）大鼠临床症状的改善效果。将28只雌性SD大鼠按每组7只随机分为正常对照组、PCOS模型组、PCOS+低剂量LTA组和PCOS+高剂量LTA组。建模阶段,除正常对照组以生理盐水灌胃外,其余3组持续灌胃来曲唑28 d诱导PCOS模型。干预阶段,正常对照组和PCOS模型组以生理盐水持续灌胃,两个LTA干预组大鼠分别持续灌胃对应剂量LTA干预30 d。结果显示,与PCOS模型组相比,LTA干预组大鼠血清中睾酮（Testosterone,T）、黄体生成素（Luteinizing hormone,LH）、胰岛素（Insulin,INS）、甘油三酯（Triglyceride,TG）、总胆固醇（Total cholesterol,TC）、低密度脂蛋白（Low-density lipoprotein cholesterol,LDL-C）、脂多糖（Lipopolysaccharide,LPS）水平及胰岛素抵抗指数（HOMA-IR）均显著降低（P<0.05）,高密度脂蛋白（High-density lipoprotein cholesterol,HDL-C）水平均显著升高（P<0.05）,黄体生成素与卵泡刺激素的比值（LH/FSH）、空腹血糖（Fasting plasma glucose,FPG）和体重均有所下降（P>0.05）;低剂量LTA干预组大鼠血清雌二醇（Estradiol,E2）显著升高（P<0.05）;高剂量LTA干预组大鼠卵泡刺激素（Follicle stimulating hormone,FSH）显著升高（P<0.05）;LTA干预组大鼠的发情周期紊乱、卵巢组织多囊样病变较PCOS模型组均有一定程度的改善。结果表明,LTA干预能有效调节PCOS大鼠性激素分泌、恢复发情周期规律变化和改善其卵巢多囊样病变,同时显著改善PCOS大鼠胰岛素抵抗（Insulin resistance,IR）、血脂代谢紊乱并抑制异常水平的脂多糖（Lipopolysaccharide,LPS）分泌。 相似文献
17.
《中国油料作物学报(英文)》2020,5(4):205-212
Lard, a fat rich in saturated fatty acids (SFAs), is regarded as a risk factor for metabolic diseases. In the present study, effect of different lard blended with sunflower oil diets on lipid accumulation in adipose tissue, liver, and serum by mouse model was researched. Body weight, body fat percentage, cross-sectional area of adipocytes, liver triglycerides (TGs), and oil red stained area in mice liver of lard blend sunflower oil (L-SFO) group were significantly lower than those of sunflower oil (SFO) group, whereas no significant differences were observed between mice of lard and L-SFO groups. Serum TG and free fatty acid levels were significantly lower in L-SFO group than in other two groups. Furthermore, data showed that sunflower oil decreased contents of hormone-sensitive lipase and carnitine palmitoyl transferase 1 (CPT-1) and increased fatty acid synthase activity in liver tissue. A mixture of lard and sunflower oil rather than only sunflower oil or lard might promote body fat loss and reduce lipid accumulation in adipose tissue, serum, and liver by promoting hydrolysis of TG, increasing β-oxidation of fatty acids. These data suggested that mixing lard and vegetable oil (e.g. sunflower oil) for cooking, or alternate using lard and vegetable oil could be beneficial for reducing body fat. 相似文献
18.
Increasing energy expenditure (EE) is beneficial for preventing obesity. Diet-induced thermogenesis (DIT) is one of the components of total EE. Therefore, increasing DIT is effective against obesity. We examined how much fish oil (FO) increased DIT by measuring absolute values of DIT in mice. C57BL/6J male mice were given diets of 30 energy% fat consisting of FO or safflower oil plus butter as control oil (Con). After administration for 9 days, respiration in mice was monitored, and then the data were used to calculate DIT and EE. DIT increased significantly by 1.2-fold in the FO-fed mice compared with the Con-fed mice. Body weight gain was significantly lower in the FO-fed mice. FO increased the levels of uncoupling protein 1 (Ucp1) mRNA and UCP1 protein in brown adipose tissue (BAT) by 1.5- and 1.2-fold, respectively. In subcutaneous white adipose tissue (subWAT), the levels of Ucp1 mRNA and UCP1 protein were increased by 6.3- and 2.7-fold, respectively, by FO administration. FO also significantly increased the expression of markers of browning in subWAT such as fibroblast growth factor 21 and cell death-inducing DNA fragmentation factor α-like effector a. Thus, dietary FO seems to increase DIT in mice via the increased expressions of Ucp1 in BAT and induced browning of subWAT. FO might be a promising dietary fat in the prevention of obesity by upregulation of energy metabolism. 相似文献
19.
藤茶总黄酮及二氢杨梅素降血脂作用研究 总被引：16，自引：0，他引：16
本文探讨了藤茶中主要活性成分黄酮及二氢杨梅素对高血脂症小鼠的降血脂效果。结果表明:与高脂模型组比较,二氢杨梅素对高脂血症小鼠体重有明显减轻作用;黄酮及二氢杨梅素都能不同程度降低小鼠血清TC、TG、LDL-C、MDA含量,提高血清HDL-C含量及SOD活性,降低肝系数。肝脏病理学观察表明,喂饲藤茶黄酮及二氢杨梅素组小鼠肝细胞变性、肿胀的程度减轻。说明藤茶黄酮及二氢杨梅素能降低小鼠血脂,增强机体抗氧化能力,减轻高脂对肝细胞的损伤作用。 相似文献
20.
R. W. Peace G. Sarwar H. G. Botting S. P. Touchburn 《Plant foods for human nutrition (Dordrecht, Netherlands)》1992,42(4):337-349
Weanling male Wistar rats were fed 20% protein diets based on casein or either of two combinations of soy protein isolate and ground raw soy providing three levels of soybean trypsin inhibitors (SBTI; 0,448 and 808 mg of trypsin inhibited per 100g of diet respectively). DL-ethionine was included at three levels (0,0.05% and 0.10%) with each level of SBTI. After 4, 8 and 12 weeks ofad libitum feeding, diets containing SBTI without DL-ethionine were associated with decreases in weight gain, feed efficiency, serum cholesterol and serum urea nitrogen. Higher levels of triglycerides, glutamate pyruvate transaminase (SGPT) and altered serum free amino acid levels were also found. Increased dietary levels of DL-ethionine also resulted in deficits in growth and feed efficiency, decreased serum cholesterol, increased SGPT and similar alterations in serum free amino acids. Combination of dietary SBTI with DL-ethionine resulted in even greater growth deficits and serum cholesterol decreases as well as increases in SGPT and serum triglycerides and changes in serum free amino acid levels. Methionine deficiency in the young rats fed SBTI and DL-ethionine was indicated by the changes in serum amino acids and growth deficits. Moderation of some effects over the 12 week test period suggested decreased methionine requirements in the older rats. 相似文献