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1.
The human cytochrome P450 CYP1A1 gene was introduced into rice plants (Oryza sativa cv. Nipponbare). One-month-old CYP1A1 plants grown in soil clearly showed a healthy growth and tolerance to 8.8 microM atrazine and 50 microM simazine, but nontransgenic plants were completely killed by the herbicides. Although transgenic and nontransgenic plants metabolized the two herbicides into the same sets of compounds, CYP1A1 plants metabolized atrazine and simazine more rapidly than did control plants. In small-scale experiments, residual amounts of atrazine and simazine in the culture medium of CYP1A1 plants were 43.4 and 12.3% of those in control medium; those of nontransgenic Nipponbare were 68.3 and 57.2%, respectively. When cultivated in soil with 2.95 microM atrazine and 3.15 microM simazine for 25 days, CYP1A1 plants eliminated 1.3 times more atrazine and 1.4 times more simazine from the soil than did control plants. Thus, CYP1A1 rice plants make it possible to remove atrazine and simazine more rapidly from the culture medium and soil than can nontransgenic Nipponbare.  相似文献   

2.
Atrazine and metolachlor are commonly detected in surface water bodies in southern Louisiana. These herbicides are frequently applied in combination to corn, and atrazine to sugarcane, in this region. A study was conducted on the runoff of atrazine and metolachlor from 0.21 ha plots planted to corn on Commerce silt loam, a Mississippi River alluvial soil. The study, carried out over a three-year period characterized by rainfall close to the 30-year average, provided data on persistence in the surface soil (top 2.5 cm layer) and in the runoff active zone of the soil, as measured by decrease in runoff concentrations with time after application. Regression equations were developed that allow an estimate of the runoff extraction coefficients for each herbicide. Atrazine showed soil half-lives in the range 10.5-17.3 days, and metolachlor exhibited half-lives from 15.8-28.0 days. Concentrations in successive runoff events declined much faster than those in the surface soil layer: Atrazine runoff concentrations decreased over successive runoff events with a half-life from 0.6 to 5.7 days, and metolachlor in runoff was characterized by half-lives of 0.6-6.4 days. That is, half-lives of the two herbicides in the runoff-active zone were one-tenth to one-half as long as the respective half-lives in the surface soil layer. Within years, the half-lives of these herbicides in the runoff active zone varied from two-thirds longer for metolachlor in 1996 to one-fifth longer for atrazine in 1995. The equations relating runoff concentrations of atrazine and metolachlor to soil concentrations contain extraction coefficients of 0.009. Losses in runoff for atrazine were 5.2-10.8% of applied, and for metolachlor they were 3.7-8.0%; atrazine losses in runoff were 20-40% higher than those for metolachlor. These relatively high percent of application losses indicate the importance of practices that reduce runoff of these chemicals from alluvial soils of southern Louisiana.  相似文献   

3.
本试验以日光温室秋冬茬番茄-冬春茬黄瓜轮作体系为研究对象,采用田间小区试验,研究了5季节水控肥（冬春茬黄瓜和秋冬茬番茄季N-P2O5-K2O总投入量分别为600-300-525 kg/hm2和450-225-600 kg/hm2）有机无机肥配施对 040 cm（根区）土壤硝态氮供应、 40100 cm（根区以外）硝态氮残留和 0100 cm土体不同形态氮素淋失的影响,探索了设施蔬菜生产中节水节肥潜力,为构建设施蔬菜合理水肥管理下土壤肥力培育和土壤质量提升模式提供技术支持。试验结果表明, 1）农民习惯水肥管理节水节肥潜力较大; 节水控肥后0100 cm土体硝态氮积累量、 矿质氮和有机氮渗漏量均明显下降,种植蔬菜经济效益显著增加。2）商品有机肥猪粪与化肥在土壤无机氮供应方面的效果接近; 节水控肥1/41/2 猪粪氮替代1/41/2 化肥氮后,040 cm土体硝态氮供应和40100 cm土体硝态氮残留均无显著变化,但是随着猪粪氮配施比例的增加,土壤溶液渗漏量及养分淋失量呈增加趋势。3）施用秸秆促进了土壤无机氮固持,降低根区土壤硝态氮供应水平,提高土壤养分保蓄能力; 节水控肥1/2秸秆氮替代1/2化肥氮后,040 cm土壤硝态氮供应量平均下降34.3%~56.2%,40100 cm土体硝态氮残留量下降42.5%~87.8%, 0100 cm土体土壤溶液渗漏量下降65.0%,硝态氮淋失量下降 82.0%,而产量和经济收入无显著差异。根据本试验结果,对于新建温室可采用单施化肥、 化肥与猪粪配施方案,能在短时间内提高土壤无机氮供应强度,满足蔬菜氮素需求; 对于种植了一段时间的温室,可采用冬春茬黄瓜季化肥配施猪粪秋冬茬番茄季化肥配施秸秆方案,能固定积累于土壤中的无机氮,提高土壤养分容量,保证根层土壤氮素的稳定供应,降低环境风险,维护设施农业的可持续发展。  相似文献   

4.
Adsorption and desorption of the herbicides glyphosate [N-phosphonomethyl-aminoaceticacid], simazine [6-chloro-N,N′-diethyl-1,3,5-triazine-2,4-diamine] and atrazine [6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine] were studied in four sandy soils from Western Australia. Distribution coefficients (Kds) were calculated from breakthrough curves (BTCs) resulting from leaching step changes in concentrations through small saturated columns of soil at flow rates ranging from 0.3 to 30 m day–1. A comparison was made with Kds obtained after batch equilibrating solutions of the herbicides with the same soils. The Kds of herbicides in soils decreased with increasing flow rate and most strongly for glyphosate in soils rich in clay content. Resulting increases in mobility of about 40–50% were estimated for simazine and atrazine and > 50% for glyphosate at flow rates of 3 m day–1. Adsorption and desorption rates were estimated by fitting numerically simulated BTCs to experimental BTCs. Best fits were obtained with a time-dependent Freundlich adsorption equation. The resulting coefficient for time dependency in the equation suggests that the rates of adsorption and desorption are controlled mainly by diffusion in an adsorbing layer on or in soil particles.  相似文献   

5.
A multiresidue method was developed for the simultaneous determination of low parts per billion (ppb) concentrations of the herbicides alachlor, metolachlor, atrazine, and simazine in water and soil using isotope dilution gas chromatography/mass spectrometry (GC/MS). Known amounts of 15N,13C-alachlor and 2H5-atrazine were added to each sample as internal standards. The samples were then prepared by a solid phase extraction with no further cleanup. A high resolution GC/low resolution MS system with data acquisition in selected ion monitoring mode was used to quantitate herbicides in the extract. The limit of detection was 0.05 ppb for water and 0.5 ppb for soil. Accuracy greater than 80% and precision better than 4% was demonstrated with spiked samples.  相似文献   

6.
Influence of fertilization on nitrogen leaching after cultivation of maize for silage over four successive seasons In a field trial, nitrogen leaching from soil was determined between February 1983 and May 1986 by analyzing soil water from 50, 80 and 110 cm below the soil surface every 14 days. On a Stagno-gleyic Luvisol, maize after maize was cultivated over four successive seasons. Nitrogen was applied either minerally in spring according to Nmin or as a semiliquid cattle manure. The time of application (autumn and/or spring), application rate and use of nitrification inhibitor dicyandiamide (DCD) were varied. Under very low N-fertilization (underground fertilization only), nitrate nitrogen losses by leaching dropped from 100 kg N/ha in the first year to 33 kg N/ha in the 3rd. Nitrogen leaching from the various treatment plots depended on the maize growth and rainfall conditions. Because of an intensive and long lasting seepage of gravitational water, nitrogen leaching from the root zone ranged from 113 to 208 kg N/ha during the fall and winter seasons of 1983/84 and 1984/85. Under the more balanced infiltration conditions of the leaching period 1985/86, and after a high yield of maize in 1985, losses due to leaching were reduced to values between 69 to 108 kg N/ha. Under these experimental conditions (deliberately high quantities of semiliquid cattle manure; DCD-application in autumn) no reduction in nitrogen losses could be proved due to the addition of dicyandiamide.  相似文献   

7.
Field experiments over a 3 y period were conducted in a winter wheat‐maize double‐cropping system at the Dongbeiwang Experimental Station, Beijing, China. Three different treatments of irrigation (sprinkler “suboptimal” and “optimized”; conventional flood irrigation) and N fertilization (none, according to Nmin soil tests, conventional) were studied with respect to effects on soil water balance, nitrate leaching, and grain yield. Under sprinkler irrigation, evaporation losses were higher due to a more frequent water application. On the other hand, in this treatment nitrate leaching was smaller as compared to flood irrigation, where abundant seepage fluxes >10 mm d–1 along preferential flow paths occurred. For quantifying nitrate leaching, passive samplers filled with ion‐exchange resins appeared to be better suited than a method which combined measurements of suction‐cup concentrations with model‐based soil water fluxes. As a result of the more balanced percolation regime (compared to that under conventional flood irrigation), there was a tendency of higher salt load of the soil solution in the rooting zone. Given a seepage rate of 50 mm, a winter wheat grain production of 5–6 t ha–1 required a total water addition of about 430 mm. Fertilizer treatments >100 kg N ha–1 did not result in any additional yield increase. An even balance between withdrawing and recharge of groundwater cannot be achieved with “optimized” irrigation, but with a reduction of evapotranspiration losses, adapted cropping systems, and/or by tapping water resources from reservoirs in more distant areas with surpluses.  相似文献   

8.
Application of water-saving irrigation technologies in transplanted rice (TPR) cultivation resulted in different soil water regimes compared to traditional flood irrigation and consequent diverse nitrogen transport and losses in paddy fields. In this study, nitrogen transport and transformations in a TPR field under multiple shallow irrigation (MSI) conditions in the Taihu Lake Basin of Eastern China were observed and simulated (Hydrus-1D model) during the 2008 and 2009 seasons based on a previous study. MSI controlled well the depth of floodwater and reduced nitrogen losses substantially through percolation and surface runoff, in particular during the 2008 season with relative less rainfall. Nitrogen balance analysis showed that both denitrification and NH3 volatilisation were the two major paths of nitrogen loss during the two seasons. Most nitrogen transformations occurred in top soil (0–40?cm) during early-middle seasons. The overall nitrification and denitrification differed slightly between the two seasons but largely between soil depths, averagely 92.7% and 73.0% of respective total in a 120?cm soil profile occurred in 0–40?cm soil, respectively. MSI method coupled with deep applied fertiliser is recommended to substantially reduce nitrogen losses through surface runoff, percolation, and NH3 volatilisation in TPR fields.  相似文献   

9.
Abstract. Nitrate leaching and soil mineral N status under grassland were measured on three contrasting soils, spanning winters 1995/96, 1996/97 and 1997/98, in Western England. The soils investigated were a freely draining silty clay loam (Rosemaund), a well drained loam (IGER 1) and a poorly drained clay loam (IGER 2). The effects of reseeding (ploughing and resowing grass) at IGER 1 and IGER 2 in autumn 1995 or 1996 were compared with undisturbed pasture. Reseeding at Rosemaund, in autumns 1995 or 1996, or spring 1996 was compared with undisturbed pasture of 3 sward ages (2, 5, >50 years).
Nitrate-N leaching losses during the winter immediately following autumn reseeding ranged between 60 and 350 kg N ha–1 in 1995/96, depending on soil type, sward management history and rainfall. Losses were much less in the following winter when treatments were repeated (10–107 kg N ha–1).
Reseeding in spring had little effect on soil mineral N content or leaching losses in the following autumn, compared with undisturbed pasture. Similarly, leaching losses from autumn reseeds in the second winter after cultivation were the same as undisturbed pasture (1-19 kg N ha–1). The effect of ploughing grassland for reseeding was relatively short-term, in contrast to the effect of repeated annual cultivation associated with arable rotations.  相似文献   

10.
Triazine herbicides are among the most widely used herbicides in the United States. Many triazine compounds are relatively stable under natural conditions and have become prominent contaminants in hydrologic systems. It was previously reported that chloro-s-triazine compounds were rapidly dechlorinated in water by polysulfides, and the reaction was assumed to be aromatic nucleophilic substitution (SNAr). In this study, we evaluated the effect of free radical inhibitors on the reaction rate of polysulfides with herbicides atrazine, simazine, and cyanazine. The reaction was significantly inhibited by radical scavengers oxygen and 1,4-benzoquinone, suggesting involvement of free radicals in the reaction. Spectral analysis of the reaction mixture using electron spin resonance showed that after the reaction, the free radical concentration in polysulfide solution substantially decreased. These evidences indicate that radical sulfur anions may also be involved in the reaction, likely via a free radical substitution reaction (SRN1) mechanism. Amendment of sodium tetrasulfide significantly reduced the leaching of atrazine or simazine from packed sand columns. Therefore, polysulfide salts may be potentially used to remove residues of triazine herbicides in environmental media.  相似文献   

11.
The effects of 20 herbicides on denitrification of nitrate in three soils were studied by determining the effects of 10 and 50μgg?1 soil of each herbicide on the amounts of nitrate lost and the amounts of nitrite, N2O and N2 produced when soil samples were incubated anaerobically after treatment with nitrate. The herbicides used were butylate, EPTC, chlorpropham, propham, diuron, linuron, monuron, siduron, alachlor, trifluralin, 2,4-D amine, 2,4-D ester, atrazine, cyanazine, metribuzin, simazine, dalapon, chloramben, dicamba and dinoseb.None of the herbicides studied significantly affected denitrification of nitrate when applied at the rate of 10 μg g?1 soil, but dinoseb increased the ratio of N2 to N2O in the gaseous products of denitrification when applied at this rate. Butylate, EPTC, diuron, simazine and dalapon had no significant effect on denitrification when applied at the rate of 50μgg?1 soil, whereas metribuzin and dinoseb enhanced denitrification when applied at this rate. The influence of the other herbicides on denitrification when applied at the rate of 50μgg?1soil depended on the soil, but all enhanced or inhibited denitrification in at least one soil.  相似文献   

12.
The transportation pathways of triazines were investigatedfrom their origins onwards, in the Marne and the Seine rivers(France). According to the 1991 survey, the highest total triazine concentration levels (956 ng L-1) were found in the river Marne: 580 ng L-1 for atrazine and 200 ng L-1 for simazine, in relation with agricultural practices.The total triazine levels reached 829 ng L-1 in the riverSeine. The other triazines remained low, from 5 to 75 ng L-1. The triazine fluxes were 226 and 404 kg for a 118 d period in the Marne and the Seine rivers, respectively. Out of the treatment period, triazine peaks were related to riverflows with a 48 hr delay between the peak and the top of theflood. After herbicide treatment, peak concentrations wereclosely related with the precipitations. Concerning theriver Marne tributaries, highest atrazine concentrationswere found in June, in those which drained the vineyardarea. In January, the concentrations were higher inperiurban areas underlining their contribution to theherbicide inputs in the rivers. On the whole, the ratio oftriazines transported in the two rivers represented 0.2%of the total amount applicated annually on their catchment basins.  相似文献   

13.

Purpose

Laboratory experiments were conducted to examine the potential for metal (Cu, Ni and Zn) and herbicide (simazine, atrazine and diuron) release from agricultural soil and dredged sediment in managed realignment sites following tidal inundation.

Materials and methods

Column microcosm and batch sorption experiments were carried out at low (5?practical salinity units, psu) and high (20?psu) salinity to evaluate the changes in the partitioning of metals and herbicides between the soil/sediment and the aqueous phase, and the release of metals and herbicides from soil/sediment to the overlying water column.

Results and discussion

For both the metals and herbicides, the highest contaminant loads were released from the sediment within the first 24?h of inundation suggesting that any negative impacts to overlying water quality in a managed realignment scheme will be relatively short term following tidal inundation of soil and sediment. The release of metals was found to be dependent on a combination of salinity effects and the strength of binding of the metals to the soil and sediment. In the case of the herbicides, salinity impacted on their release. Particulate organic carbon was found to control the binding and release of the herbicides, highlighting the importance of assessing soil and sediment organic matter content when planning managed realignment sites.

Conclusions

Our research demonstrates that metals and herbicides may be released from contaminated sediments and agricultural soils during initial periods of flooding by seawater in managed realignment sites.  相似文献   

14.
Biochar mediated alterations in herbicide breakdown and leaching in soil   总被引：3，自引：0，他引：3  
Biochar application to soil has been proposed as a mechanism for improving soil quality and the long term sequestration of carbon. The implications of biochar on pesticide behavior, particularly in the longer term, however, remains poorly understood. Here we evaluated the influence of biochar type, time after incorporation into soil, dose rate and particle size on the sorption, biodegradation and leaching of the herbicide simazine. We show that typical agronomic application rates of biochar (10-100 t ha−1) led to alterations in soil water herbicide concentrations, availability, transport and spatial heterogeneity. Overall, biochar suppressed simazine biodegradation and reduced simazine leaching. These responses were induced by a rapid and strong sorption of simazine to the biochar which limits its availability to microbial communities. Spatial imaging of 14C-labeled simazine revealed concentrated hotpsots of herbicide co-localized with biochar in the soil profile. The rate of simazine mineralization, amount of sorption and leaching was inversely correlated with biochar particle size. Biochar aged in the field for 2 years had the same effect as fresh biochar on the sorption and mineralization of simazine, suggesting that the effects of biochar on herbicide behavior may be long lasting. We conclude that biochar application to soil will reduce the dissipation of foliar applied pesticides decreasing the risk of environmental contamination and human exposure via transfer in the food chain, but may affect the efficacy of soil-applied herbicides.  相似文献   

15.
Most farming systems involving tilled crops require use of pesticides and nitrogen fertilizers in different combinations although most pesticides effects on soil N transformation are scantly documented. Studies were initiated to compare atrazine and simazine herbicides with two biological nitrification inhibitors (nitrapyrin and terrazole) for their effects on biological nitrification and corn (Zea mays L.) growth. In a laboratory study, inhibition of nitrification was less than 3% in a Tifton loamy sand incubated with 10 μg a.i g‐1 soil atrazine or simazine but was more than 10% in soil amended with nitrapyrin or terrazole, applied separately or in combinations with either herbicide at the same rate. Similar trends were observed with soil treated with different combinations of 2.5 μg a.i. g‐1 soil nitrapyrin or terrazole and 1.25 μg a.i. g‐1 soil atrazine or simazine and incubated with and without corn plants under greenhouse conditions. The combination of either herbicide with nitrapyrin or terrazole significantly reduced the corn dry weights with substantial accumulation of Kjeldahl N and NO3 ‐ in tissues of plants, probably due to a concentration effect. However, these chemical combinations, applied at the rate of 1.2 kg a.i. ha‐1 in conjunction with 35 kg ha‐1 N as (NH4)2SO4 in split banded applications (at planting and at the 6th leaf stage), showed a nonsignificant trend towards increased corn ear yields in two‐year field studies. Generally, when atrazine or simazine was part of the chemical treatment, its effects on nitrification, plant growth and total N contents outweighed or masked those of nitrapyrin or terrazole.  相似文献   

16.
Abstract. Losses of soil and fertilizer nitrogen by leaching and denitritication from a clay soil in southern England have been measured over four years. Nitrate losses in drainage water from direct-drilled land averaged 20–30 kg N ha 'a' with wide seasonal variation. Ploughing and conventional cultivations increased this loss. Denitritication from direct-drilled land averaged 5–10 kg N ha 'a' with wide seasonal variation. Ploughing and drainage both diminished denitritication losses but cultivation had the greater effect. These nitrogen losses occurred mainly in autumn and spring.
Nitrogen losses, in drainage water or by denitritication after spring fertilizer applications, were related to the rainfall in the 28 days following top dressing. Approximately 40 mm rain was needed to cause a loss of 10% of the nitrogen applied but in practice losses were quite variable.  相似文献   

17.
Abstract. We examined the effect on soil nutrient status and sustainability of water percolation through an irrigated paddy field in Japan, to the depth of drainage (40 cm). The difference between amounts of nutrients leached by percolation and those supplied by irrigation indicated that 25–130 kg ha−1 Ca, 8–24 kg ha−1 Mg, from −1 to 9 kg ha−1 K, and 8–17 kg ha−1 Fe, respectively, were lost each year from the 0–40 cm soil layer during rice cultivation, when the supply from fertilization and rainfall and the loss in grain harvest were not accounted for. When the supply of K from rainfall and the loss in grain harvest were taken into account, a total K loss of about 10 kg ha−1 was estimated. The electrical neutrality of inorganic ions in the percolating water was always maintained. From these results we estimate that the amounts of exchangeable Ca and Mg in the soil to a depth of 40 cm would decrease by 50% within 50–260 and 30–100 years, respectively, if similar management were continued without fertilization. The total amount of carbon dioxide (ΣCO2) leached in percolating water during the period of rice cultivation was 120–325 kg C ha−1, which corresponded to 0.47–0.94% of the soil organic carbon to 40 cm depth.  相似文献   

18.
The photodegradation of three triazines, atrazine, simazine, and prometryn, in aqueous solutions and natural waters using UV radiation (lambda > 290 nm) has been studied. Experimental results showed that the dark reactions were negligible. The rate of photodecomposition in aqueous solutions depends on the nature of the triazines and follows first-order kinetics. In the case of the use of hydrogen peroxide and UV radiation, a synergistic effect was observed. The number of photodegradation products detected, using FIA/MS and FIA/MS/MS techniques, suggests the existence of various degradation routes resulting in complex and interconnected pathways.  相似文献   

19.
The impact of incorporated residues of winter oilseed rape, peas and oats on soil N availability and the risk of N leaching during autumn and winter in a northern climate is not clear. Therefore, the aim was to determine the influence of incorporated residues on net N mineralization–immobilization in topsoil during autumn and winter. A field experiment carried out at three sites in South Sweden provided soil samples and crop residues for an interpretive, in situ incubation study. Topsoil corresponding to a 7‐cm soil layer from each site used for the field experiment was incubated with and without aboveground residues under natural temperature conditions at a single field location. On the basis of the incubation study, we concluded that in the field experiment, soil N dynamics during autumn and winter trials were the combined outcome of net N mineralization in the topsoil fraction not affected by aboveground residues and net N immobilization in the fraction in contact with aboveground crop residues. In the absence of aboveground residues, the net rate of N mineralization during early autumn was similar after both oilseed rape and peas, but values were larger than that after oats. After incorporation, aboveground residues of winter oilseed rape and peas made no contribution to soil mineral N in late autumn and thus did not increase the risk of N losses during winter. In fact, the residues of oilseed rape, peas and oats reduced the amount of soil mineral N by 7–14 kg N/ha during the main drainage period (October–March). Therefore, incorporating chopped aboveground residues should be encouraged before sowing winter wheat after peas and winter oilseed rape.  相似文献   

20.
The effect of light and temperature on the dissipation of four s-triazine herbicides (terbuthylazine, simazine, atrazine and prometryn) was studied in drinking and wastewaters during long-term laboratory incubation (4 months), and by comparing the results with those obtained in purified water (Milli-Q). Residues were analyzed by GC-NPD and confirmed by GC-MSD. A micro on-line method for isolating the herbicide residues was used. The results showed that temperature and light had a certain influence on the behaviour of the s-triazine herbicides. In drinking water, prometryn dissipated more rapidly than the other compounds under all laboratory conditions (t 1/2= 75–128 days), while atrazine showed the highest degree of persistence (t 1/2= 132–227 days). In general, atrazine was the least (t 1/2= 90–142 days) and simazine the most (t 1/2= 118–278 days) persistent compound in Milli-Q and wastewater. Only in the case of atrazine in drinking water was the remaining percentage at the end of the experiment higher than 50% (53–69%). Dissolved organic substances in wastewater (DOC, 53.3 mg l–1) appear to be particularly important in the photosensitization process. The time required to reach the maximum admissible concentration (MAC) according to European legislation (0.1 g l–1) ranged from 3 to 10 years for prometryn and atrazine, respectively, in drinking water depending on the used conditions.  相似文献   

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