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1.
Wellehan JF Green LG Duke DG Bootorabi S Heard DJ Klein PA Jacobson ER 《Comparative immunology, microbiology and infectious diseases》2009,32(5):379-394
Megachiropteran bats are biologically important both as endangered species and reservoirs for emerging human pathogens. Reliable detection of antibodies to specific pathogens in bats is thus epidemiologically critical. Eight variable flying foxes (Pteropus hypomelanus) were immunized with 2,4-dinitrophenylated bovine serum albumin (DNP-BSA). Each bat received monthly inoculations for 2 months. Affinity-purified IgG was used for production of polyclonal and monoclonal anti-variable flying fox IgG antibodies. ELISA and western blot analysis were used to monitor immune responses and for assessment of polyclonal and monoclonal antibody species cross-reactivity. Protein G, polyclonal antibodies, and monoclonal antibodies detected specific anti-DNP antibody responses in immunized variable flying foxes, with protein G being the most sensitive, followed by monoclonal antibodies and then polyclonal antibodies. While the polyclonal antibody was found to cross-react well against IgG of all bat species tested, some non-specific background was observed. The monoclonal antibody was found to cross-react well against IgG of six other species in the genus Pteropus and to cross-react less strongly against IgG from Eidolon helvum or Phyllostomus hastatus. Protein G distinguished best between vaccinated and unvaccinated bats, and these results validate the use of protein G for detection of bat IgG. Monoclonal antibodies developed in this study recognized immunoglobulins from other members of the genus Pteropus well, and may be useful in applications where specific detection of Pteropus IgG is needed. 相似文献
2.
Rehtanz M Bossart GD Doescher B Rector A Van Ranst M Fair PA Jenson AB Ghim SJ 《Veterinary microbiology》2009,133(1-2):43-53
Papillomaviruses (PVs) have been shown as being the etiologic agents of various benign and malignant tumours in many vertebrate species. In dolphins and porpoises, a high prevalence of orogenital tumours has recently been documented with at least four distinct novel species-specific PV types detected in such lesions. Therefore, we generated the immunological reagents to establish a serological screening test to determine the prevalence of PV infection in Atlantic bottlenose dolphins [(Tursiops truncatus (Tt)]. Using the baculovirus expression system, virus-like particles (VLPs) derived from the L1 proteins of two TtPV types, TtPV1 and TtPV2, were generated. Polyclonal antibodies against TtPV VLPs were produced in rabbits and their specificity for the VLPs was confirmed. Electron microscopy and enzyme-linked immunosorbent assay (ELISA) studies revealed that the generated VLPs self-assembled into particles presenting conformational immunodominant epitopes. As such, these particles are potential antigen candidates for a TtPV vaccine. Subsequently, the VLPs served as antigens in initial ELISA tests using sera from six bottlenose dolphins to investigate PV antibody presence. Three of these sera were derived from dolphins with genital tumour history and showed positive PV ELISA reactivity, while the remaining sera from lesion-free dolphins were PV antibody-negative. The results suggest that the developed screening test may serve as a potential tool for determining PV prevalence and thus for observing transmission rates in dolphin populations as the significance of PV infection in cetaceans starts to unfold. 相似文献
3.
Van Bressem M Waerebeek KV Jepson PD Raga JA Duignan PJ Nielsen O Di Beneditto AP Siciliano S Ramos R Kant W Peddemors V Kinoshita R Ross PS López-Fernandez A Evans K Crespo E Barrett T 《Veterinary microbiology》2001,81(4):287-304
Serum samples from 288 cetaceans representing 25 species and originating from 11 different countries were collected between 1995 and 1999 and examined for the presence of dolphin morbillivirus (DMV)-specific antibodies by an indirect ELISA (iELISA) (N = 267) or a plaque reduction assay (N = 21). A total of 35 odontocetes were seropositive: three harbour porpoises (Phocoena phocoena) and a common dolphin (Delphinus delphis) from the Northeastern (NE) Atlantic, a bottlenose dolphin (Tursiops truncatus) from Kent (England), three striped dolphins (Stenella coeruleoalba), two Risso's dolphins (Grampus griseus) and a bottlenose dolphin from the Mediterranean Sea, one common dolphin from the Southwest (SW) Indian Ocean, three Fraser's dolphins (Lagenodelphis hosei) from the SW Atlantic, 18 long-finned pilot whales (Globicephala melas) and a bottlenose dolphin from the SW Pacific as well as a captive bottlenose dolphin (Tursiops aduncus) originally from Taiwan. The presence of morbillivirus antibodies in 17 of these animals was further examined in other iELISAs and virus neutralization tests. Our results indicate that DMV infects cetaceans worldwide. This is the first report of DMV-seropositive animals from the SW Indian, SW Atlantic and West Pacific Oceans. Prevalence of DMV-seropositives was 85.7% in 21 pilot whales from the SW Pacific and both sexually mature and immature individuals were infected. This indicates that DMV is endemic in these animals. The same situation may occur among Fraser's dolphins from the SW Atlantic. The prevalence of DMV-seropositives was 5.26% and 5.36% in 19 common dolphins and 56 harbour porpoise from the NE Atlantic, respectively, and 18.75% in 16 striped dolphins from the Mediterranean. Prevalence varied significantly with sexual maturity in harbour porpoises and striped dolphins; all DMV-seropositives being mature animals. The prevalence of seropositive harbour porpoise and striped dolphins appeared to have decreased since previous studies. These data suggest that DMV is not endemic within these populations, that they are losing their humoral immunity against the virus and that they may be vulnerable to new epidemics. 相似文献
4.
WANG Leming WANG Yurui ZENG Zixuan RAO Guoshun WU Zhengjiao JIN Weikun WANG Dongying 《中国畜牧兽医》2022,49(2):677-686
【Objective】 This study was intend to obtain cathepsin L1(rFgCat L1) specific monoclonal antibody and construct the double antibody sandwich ELISA.【Method】 Five BALB/c mice were immunized with 1 mg/mL rFgCat L1 protein for four times.Mouse splenocytes were isolated and fused with SP2/0 cells to construct hybridoma cells.Strong positive hybridoma cell lines were screened, 1×106 cells were injected intraperitoneally per mouse to prepare monoclonal antibodies.Antibody titer and antigenic epitope were detected using ELISA method, antibody subtype and specificity were identified using Western blotting method.The double antibody sandwich ELISA was constructed by combining the anti-rFgCat L1 polyclonal antibody, and its sensitivity and specificity were tested.The positive and negative critical value was screened by 20 negative sera with positive control, and the constructed double antibody sandwich ELISA was verified by 47 goat positive sera and 47 dairy cow positive sera.【Result】 After immunization, the antibody titers in serum of 4 mice were all more than 104.After isolated mouse with the highest immune response spleen cells were fused with SP2/0 cells total of 8 of them were positive cell lines were obtained after selective culture.5D5 and 7G6 were identified as strong positive strains with stable antibody secretion.After multiple subcloning screens and subcultures, the antibodies secreted in the cell supernatant were stable, with titers of 29 and 210 respectively, with ascites titers of 107 and 108.Western blotting and antibody subtype identification kits identified that the two antibodies were IgG1 type and the light chain was kappa type, both of which could specifically bind FgESP.According to the same antigen site was recognized by the two kinds of antibodies, the antigen titer of the two monoclonal antibodies were comparied, 7G6 was used as the coating antibody, and anti-rFgCat L1 was used as the enzyme-labeled secondary antibody.The optimized condition of method was that 7G6 was coated at a concentration of 2 μg/mL, the dilution concentration of anti-rFgCat L1 polyclonal antibody was 25 μg/mL, the dilution of Don-HRP-conjugated was 1∶4 000, 5% skimmed milk powder was selected as the blocking solution and the color development time was 25 min.The method was proved that could recognize the lowest antigen concentration of 0.625 μg/mL, also could specifically recognize antigen of Fasciola fasciatus.The constructed sandwich ELISA method was used for antigen detection of 47 dairy cow positive serum and 47 goat positive serum infective samples kept in the laboratory and the positive antigen rate were 72.3% and 78.7%, respectively.【Conclusion】 Anti-rFgCat L1 monoclonal antibody was successfully prepared and the double-sheet sandwich ELISA method for fascioliasis was constructed, which provided a good theoretical basis and material basis for the development of low-cost and rapid diagnostic kits. 相似文献
5.
OBJECTIVE: To develop mouse monoclonal and rabbit polyclonal antibodies against immunoglobulin of Argentine boa constrictors and to demonstrate the ability of these reagents to detect antibody responses in boa constrictors by use of an ELISA and western blot analysis. ANIMALS: Two 3-year-old Argentine boa constrictors. Procedure-Boa constrictors were immunized with 2,4-dinitrophenylated bovine serum albumin (DNP-BSA). Each snake received biweekly inoculations of 250 microg of DNP-BSA (half SC, half IP) for a total of 6 inoculations followed by monthly inoculations for 3 months. Preimmune blood samples were collected. Subsequently, blood was collected immediately prior to each booster inoculation. Anti-DNP antibodies were isolated from immune plasma samples by affinity chromatography. Affinity-purified boa anti-DNP immunoglobulin was used for production of polyclonal and monoclonal antibodies. An ELISA and western blot analysis were used to monitor immune responses, for purification of boa anti-DNP immunoglobulin, and for assessment of polyclonal and monoclonal antibody specificity. RESULTS: A 6-fold increase in optical density (OD405) of immune boa plasma, compared with preimmune plasma, was detected by the polyclonal antibody, and a 12- and 15-fold increase was detected by monoclonal antibodies HL1787 and HL1785, respectively, between weeks 4 and 8. Results of western blot analysis confirmed anti-DNP antibody activity in immunized boa plasma and in affinity column eluates. Polyclonal and monoclonal antibodies detected specific anti-DNP antibody responses in immunized boas. CONCLUSIONS AND CLINICAL RELEVANCE: Polyclonal and monoclonal antibodies recognized boa constrictor immunoglobulin. These antibodies may be useful in serologic tests to determine exposure of snakes to pathogens. 相似文献
6.
为研究日本血吸虫凋亡蛋白抑制因子（inhibitor of apoptosis protein of Schistosoma japonicum,SjIAP）重组蛋白诱导BALB/c小鼠的免疫保护效果,利用PCR技术扩增SjIAP基因,构建重组表达质粒pET-28a（＋）-SjIAP,诱导表达重组SjIAP蛋白,并利用重组蛋白制备兔源多克隆抗体血清。然后,选用SjIAP重组蛋白免疫BALB/c小鼠,利用ELISA检测免疫小鼠血清中的特异性抗体水平,以及免疫小鼠脾脏淋巴细胞在SjIAP重组蛋白刺激后产生的细胞因子水平。强化免疫后,将小鼠进行血吸虫尾蚴攻虫试验,感染38 d,进行剖杀,计算虫体减虫率及肝脏减卵率。Western blot结果表明本研究制备的兔源多抗血清能特异性识别SjIAP重组蛋白。ELISA检测表明免疫SjIAP重组蛋白可诱导较高水平的IgG及IgG亚型（IgG1、IgG2a、IgG2b、IgG3）抗体和IFN-γ、IL-2及IL-4细胞因子。动物试验表明,免疫SjIAP重组蛋白的小鼠与PBS组相比分别获得了31.5%的减虫率和37.2%的肝脏减卵率。免疫SjIAP重组蛋白能诱导小鼠获得一定减虫和减卵保护效果,提示血吸虫凋亡蛋白抑制因子可作为抗血吸虫病的疫苗候选分子。 相似文献
7.
为获得分泌抗大豆凝集素(SBA)单克隆抗体的杂交瘤细胞,以纯化的SBA为抗原,免疫BALB/c小鼠,加强免疫3 d后取小鼠脾细胞与骨髓瘤细胞(SP2/0)融合,应用有限稀释法和间接ELISA 方法克隆筛选出2株分泌抗SBA单克隆抗体的杂交瘤细胞系A7、F12。细胞上清抗体效价均在1∶2×103以上,腹水抗体效价均为1∶1×106,单抗亚型鉴定结果均为IgG2b型,分子质量为189.6 ku,亲和常数为7.1×107 mol/L,Western blotting结果表明,2株单抗具有较高的特异性。该McAb的制备为建立SBA定量检测方法奠定了基础。 相似文献
8.
鸡抗REV血清的制备与检定 总被引：1，自引：1，他引：0
用网状内皮组织增生症病毒（REV）HA9901株免疫SPF鸡,无菌采血制备鸡抗REV血清。通过ELISA、IFA、AGP、HI等方法对制备的血清进行检测,未检出禽类常见的其他17种（或亚型）病毒的抗体,该血清具有良好的特异性。通过测定,该血清用于间接免疫荧光试验（IFA）的效价为1：2000,用于IFA检测REV的染色效果与REV单抗（11818和11854的混合物）相当。试验结果表明,该批血清1000倍稀释可作为一抗,用于REV的间接免疫荧光检测。 相似文献
9.
对虾白斑综合症病毒双抗体夹心ELISA方法的建立 总被引：1，自引：0，他引：1
用将纯化的对虾白斑综合症病毒VP28蛋白免疫BALB/c小鼠,分离免疫鼠脾细胞,与SP2/0细胞融合,经间接ELISA筛选,得到了2株（2G9,3E5）可以稳定分泌抗对虾白斑综合症病毒特异性单抗的杂交瘤细胞株。用纯化的VP28蛋白免疫家兔,按常规方法制备多抗。选用单抗（3E5）包被ELISA板,用兔多抗作为捕获抗体,建立了对虾白斑综合症病毒的双抗体夹心ELISA检测方法。该方法具有良好的特异性和敏感性,为对虾白斑综合症病毒的检测提供了有效工具。 相似文献
10.
Monoclonal antibodies to bovine enteric coronavirus (BEC) were produced. Additionally, polyclonal antibodies were made in rabbits and guinea pigs and extracted from the yolk of immunized hens. The antibodies were characterized by neutralization test, hemagglutination inhibition test, enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Neutralizing antibody titers of polyclonal antisera ranged from 1:1280 to 1:40,000. Only one out of 908 hybridoma colonies tested secreted antibodies with neutralizing activity. By ELISA, polyclonal sera exhibited high background reactions that could be significantly reduced by treatment with kaolin in the case of rabbit sera. Attempts to establish an ELISA for BEC antigen detection based on polyclonal sera failed due to low sensitivity and specificity. Optimal results were achieved when a mixture of two monoclonal antibodies was coated onto microplates for antigen capture, while rabbit hyperimmune serum served as detecting antibodies in an indirect assay. The combination of the two monoclonal antibodies did not increase sensitivity synergistically, but in a compensatory fashion, probably because of epitope differences between BEC field strains. 相似文献
11.
旨在制备非洲猪瘟病毒(ASFV)p54蛋白的特异性单克隆抗体。本研究利用大肠杆菌表达系统表达p54蛋白,免疫BALB/c小鼠,取其脾细胞与SP2/0细胞进行细胞融合。利用纯化的p54蛋白作为包被抗原,采用间接ELISA方法筛选获得阳性杂交瘤细胞。经4次亚克隆后,取杂交瘤细胞上清进行单克隆抗体亚型鉴定,利用体内诱生法制备单克隆抗体并进行纯化。间接ELISA方法检测单克隆抗体的效价,利用交叉反应性试验、间接免疫荧光试验和蛋白印迹对所获单克隆抗体的特异性进行鉴定。根据预测的p54蛋白二级结构,采用逐步截短法分析鉴定单克隆抗体识别的抗原表位区域,并在p54的三级结构中进行标注。结果显示：成功筛选了6株分泌p54单克隆抗体的杂交瘤细胞,分别命名为28G12-1、31G7-1、31G7-2、35F10-1、35F10-2、38D3-1。其中28G12-1、31G7-1、31G7-2重链为IgG2a型,35F10-1、35F10-2、38D3-1重链为IgG1型;轻链均为κ链。单克隆抗体的最低效价为1∶25 600,与猪繁殖与呼吸综合征病毒、猪伪狂犬病病毒、猪流行性腹泻病毒、猪细小病毒、猪急性腹泻综... 相似文献
12.
本研究拟评估仔猪接种猪繁殖与呼吸综合征减毒活疫苗对猪圆环病毒病疫苗、猪瘟疫苗免疫的干扰情况,并分析不同免疫方式对仔猪生长性能的影响,以期为探究PRRSV减毒活疫苗与PCV2、CSF疫苗的联合免疫提供数据参考。本研究先以100头仔猪为研究对象,将其随机分为A、B、C、D 4组。其中A组仔猪免疫PRRSV减毒活疫苗7 d后免疫PCV2疫苗;B组仔猪同期分点注射PRRSV减毒活疫苗和PCV2疫苗;C组仔猪仅免疫PCV2疫苗;D组仔猪仅免疫PRRSV减毒活疫苗。另外再筛选100头仔猪,随机分为E、F、G、H 4组。E组仔猪于免疫PRRSV减毒活疫苗12 d后免疫CSF疫苗;F组仔猪同期分点注射PRRSV减毒活疫苗和CSF疫苗;G组仔猪仅免疫CSF疫苗;H组仔猪仅免疫PRRSV减毒活疫苗。免疫4周后,测定仔猪血清中相关抗体水平。同时,称量免疫前后各组仔猪的体重,计算不同免疫方案仔猪的平均日增重。结果表明：A~D组中, A组和B组仔猪在免疫4周后均产生了较高水平的PRRSV及PCV2抗体,且A组抗体水平略高于B组。C组仔猪仅产生了PCV2抗体,D组仔猪产生了较高水平的PRRSV抗体。E~H 4组中,E组和F组仔猪均产生了较高水平的PRRSV及CSFV抗体。G组仔猪仅产生了高水平的CSFV抗体,H组仔猪仅产生了高水平的PRRSV抗体。A、B、C、D 4组中B组仔猪的平均日增重最高;而E、F、G、H 4组中E组仔猪的平均日增重显著高于其他3组。PRRSV减毒活疫苗与PCV2疫苗或CSF疫苗同期分点免疫、免疫PRRSV减毒活疫苗一段时间后再免疫PCV2或CSF疫苗均能诱导仔猪产生高水平的抗体;在体液免疫方面,PRRSV减毒活疫苗免疫与否均未对另外二种疫苗表现出明显的干扰作用。在仔猪28日龄时同期分点免疫PRRSV减毒活疫苗与PCV2疫苗,12 d后再免疫CSF疫苗的免疫方案不仅能诱导仔猪产生高水平抗体,还可以使仔猪具备较高的平均日增重。 相似文献
13.
Chen D Colditz IG Glenn GM Tsonis CG 《Veterinary immunology and immunopathology》2000,77(3-4):191-199
Cholera (and related) toxins (CT) when applied topically on unbroken skin induce systemic immune responses in mice, a procedure called transcutaneous immunization (TCI). The current study examined the capacity for TCI to induce systemic immune responses in sheep. Three groups (n=5 per group) were immunized at day 0 (priming) and day 28 (boosting) with 250 microg of CT in water by TCI, with 25 microg of CT in alum by intramuscular injection, or not immunized. Serum samples were taken at days 0, 28, 42, 56 and 70 after immunization for measurement of CT-specific IgG as well as CT-specific IgG1, IgG2, IgA and IgM antibodies by ELISA. After immunization, IgG, IgG1 and IgG2 antibody in immunized groups were significantly higher than in the control group, and boosting further increased these titres. IgG, IgG1 and IgG2 in the injection group were significantly higher than in the TCI group. There was a preponderance of IgG1 antibody, relative to IgG2, in both immunized groups. CT-specific IgA and IgM were detected in both immunized groups. Lymphocyte proliferation to CT was measured at day 90. A CT-specific lymphocyte proliferative response (stimulation index>2) was detected in all sheep from the injection group, in two sheep from the TCI group and in none of the controls. Results demonstrated that TCI induces primary and secondary antibody responses and specific proliferative responses to CT in sheep. 相似文献
14.
Antigen requirements and specificity of enzyme-linked immunosorbent assay for detection of canine IgG against canine distemper viral antigens 总被引：1，自引：0，他引：1
An enzyme-linked immunosorbent assay (ELISA) was developed for detection of specific immunoglobulin G (IgG) against canine distemper virus (CDV) antigens. Sucrose gradient separation of viral and cellular proteins was required to produce coating antigens for the ELISA. The specificity of the ELISA was demonstrated by blocking CDV-positive canine sera with CDV-specific antisera produced in goats and rabbits and adsorption of positive sera with CDV antigens. A comparison of the ELISA with the serum-neutralization technique for the detection of CDV antibodies was conducted. Anti-CDV IgG was detected in conventional dogs as early as 6 days after inoculation with a commercial vaccine to CDV. Paired sera from the immunized dogs were evaluated by both techniques and a statistically (P less than 0.01) significant agreement between the ELISA and the serum-neutralization technique was shown (r = 0.6121, n = 75). 相似文献
15.
Vaughan K Del Crew J Hermanson G Wloch MK Riffenburgh RH Smith CR Van Bonn WG 《Veterinary immunology and immunopathology》2007,120(3-4):260-266
The immunization of exotic species presents considerable challenges. Nevertheless, for facilities like zoos, animal parks, government facilities and non-profit conservation groups, the protection of valuable and endangered species from infectious disease is a growing concern. The rationale for immunization in these species parallels that for human and companion animals; to decrease the incidence of disease. The U.S. Navy Marine Mammal Program, in collaboration with industry and academic partners, has developed and evaluated a DNA vaccine targeting a marine viral pathogen – dolphin morbillivirus (DMV). The DMV vaccine consists of the fusion (F) and hemagglutinin (H) genes of DMV. Vaccine constructs (pVR-DMV-F and pVR-DMV-H) were evaluated for expression in vitro and then for immunogenicity in mice. Injection protocols were designed for application in Atlantic bottlenose dolphins (Tursiops truncatus) to balance vaccine effectiveness with clinical utility. Six dolphins were inoculated, four animals received both pDMV-F and pDMV-H and two animals received a mock vaccine (vector alone). All animals received an inoculation week 0, followed by two booster injections weeks 8 and 14. Vaccine-specific immune responses were documented in all four vaccinated animals. To our knowledge, this is the first report of pathogen-specific immunogenicity to a DNA vaccine in an aquatic mammal species. 相似文献
16.
以猪瘟弱毒疫苗免疫空怀母猪,待母猪分娩后,分别收集10 d内的乳汁,以建立的间接ELISA方法检测猪瘟IgA、IgG、IgM水平,并观察各种抗体动态变化规律。试验结果表明,猪初乳中的IgA、IgG、IgM抗体效价均在分娩当天达到最高值,随后迅速下降,7 d后抗体水平与常乳基本一致。对照组母猪仅在分娩当天检测到较低的抗体水平。 相似文献
17.
To obtain the anti-kappa casein monoclonal antibody and complete the identification of the antibody characteristics. The BALB/c mice were immunized with kappa casein using foot-pad immunization. Popliteal lymph node cells from the immunized mice were fused with SP2/0 myeloma cells in the presence of PEG. Three hybridoma strains (1C4, 3G3, 3E6) which secreted the antibody specific for kappa casein were obtained．The sub-class of the antibodies were IgG1. The ascites were purified by Protein G affinity layer absorption column. The antigenic epitope of 3G3 and 3E6 were different and it was close between 1C4 and 3E6.The titer of purified ascites(1C4) was 1.28×106 and the affinity constant was 2.89×108 mol/L. A anti-kappa casein monoclonal antibody with good affinity had been achieved，which provided foundations for the rapid detection of casein in bovine milk samples. 相似文献
18.
建立牛结核病的γ-干扰素(BovIFN-γ)免疫学检测方法。本研究利用rBovIFN-γ抗原免疫Balb/c小鼠,经5次免疫后,通过淋巴细胞杂交瘤技术及间接ELISA筛选制备抗BovIFN-γ的单克隆抗体,在McAb及多克隆抗体的基础上建立双抗体夹心ELISA,并进一步优化ELISA反应条件。结果表明,获得一株能稳定分泌抗BovIFN-γ McAb的杂交瘤细胞株A12E9,分泌单克隆抗体腹水效价为1∶107,属于IgG1亚类,具有良好的特异性;所建立的双抗体夹心ELISA方法,BovIFN-γ最低检测限为40 ng/mL,与其他蛋白不发生交叉反应,表现出良好的特异性。该方法为建立牛结核病的γ-干扰素诊断方法奠定了基础。 相似文献
19.
旨在制备猪RNA聚合酶Ⅱ的单克隆抗体并进行初步应用。本研究采用生物信息方法预测免疫原，将其化学合成后免疫5只4~8周龄Bal b/c雌性小鼠，对免疫后呈现阳性的小鼠进行细胞融合试验，取其脾细胞与骨髓瘤细胞进行融合并获得能稳定分泌抗RNA PolⅡ的杂交瘤细胞。鉴定结果显示，RNA PolⅡ单克隆抗体的重链为IgG 2A型，轻链为Kappa型。利用间接ELISA方法对杂交瘤细胞进行筛选和亚克隆，获得了8株稳定分泌RNA PolⅡ单克隆抗体的杂交瘤细胞株。将其初步应用到染色质免疫共沉淀（ChIP-seq）技术，并与商业化抗体的富集性进行比较，结果表明，本研究得到的单克隆抗体富集性更强。本研究获得的猪RNA PolⅡ单克隆抗体可为表观遗传学的研究提供理论基础，并且提供重要的生物学材料。 相似文献
20.
Estep JS Baumgartner RE Townsend F Pabst DA McLellan WA Friedlaender A Dunn DG Lipscomb TP 《Veterinary pathology》2005,42(3):357-359
Seminoma with metastasis was diagnosed in a spotted dolphin (Stenella frontalis) and an Atlantic bottlenose dolphin (Tursiops truncatus). Sertoli cell tumor and pheochromocytoma were also diagnosed in the spotted dolphin. The spotted and bottlenose dolphins were adult males that stranded and died on the coasts of northwest Florida and southeast North Carolina, respectively. Neoplasia is infrequently reported in cetaceans. This is the first report of seminoma, Sertoli cell tumor, and pheochromocytoma in a dolphin, the first report of three distinct neoplasms in a dolphin, and one of the few reports of malignant neoplasia in dolphins. 相似文献