共查询到19条相似文献，搜索用时 281 毫秒
1.
2.
保存温度对网纹甜瓜花粉生活力的影响 总被引：6，自引：0，他引：6
研究了网纹甜瓜花粉生活力在不同保存温度和培养温度下的变化,结果表明25-35℃培养温度范围最适合 网纹甜瓜花粉萌发,萌发下限温度为10℃,上限温度为45℃。常温(25℃)下网纹甜瓜花粉极易失去活力;5℃低温条 件下比较适合于网纹甜瓜花粉的短期保存;-20℃和-75℃低温条件下不适合网纹甜瓜花粉的保存。 相似文献
3.
4.
以大棚‘早生新水’露白期和白蕾期的花枝为试材,在25、35、40℃下分别处理不同时间来模拟高温对花粉量和花粉活力的影响。结果表明:露白期,25℃时花粉量没有明显变化,花粉萌发率增加53%以上;35℃高温下花粉量增加多达46%,每花药为5 000粒,花粉萌发率也较高;40℃高温下花粉量、花粉萌发率处理2h之后就显著下降。白蕾期比露白期的花粉量多,25℃时花粉量没有明显变化,而花粉萌发率处理6h后升高;35℃高温下花粉量明显减少,每花药为2 000粒,花粉萌发率在处理6h后急剧下降;40℃高温下花粉量和花粉萌发率均显著下降。不同温度处理后,将花枝在25℃进行修复试验(处理4h)。不同处理修复前和修复后的花粉萌发率变化不明显,因此高温对不同时期花粉活力的影响均不可逆转。与花粉量、花粉萌发率相比,高温对花粉管生长影响小。因此,在设施栽培过程中应严格管控花期的温度,尽量避免35℃以上高温长时间出现。 相似文献
5.
6.
7.
8.
研究表明，培养基中的硼酸浓度对甜瓜花粉离休萌发具有明显的影响，培养甜瓜花粉宜采用含蔗糖15％、硼酸400×10－6的液体培养基。在28±1℃条件下培养2小时，甜瓜花粉萌发率基本上达最高值，花粉管长度的相对增长率也基本下降到最低值。 相似文献
9.
10.
11.
12.
地被菊花粉活力和储藏性研究 总被引：4，自引：0，他引：4
以地被菊（groundcover chrysanthemum）品种‘毛白玉’、‘粉芙蓉’的新鲜花粉为材料,采用离体萌发法对花粉活力进行测定,并观察花粉萌发及花粉管生长情况。试验结果表明：外源Ca2+显著促进了花粉萌发和花粉管生长,硼酸对花粉萌发没有显著影响;花序中部筒状花的花粉活力显著高于花序外部和内部筒状花的花粉;遮荫降低了花粉活力;‘毛白玉’和‘粉芙蓉’花粉在室温下可分别储藏8和6 d,4 ℃下可分别储藏25 d和20 d,–20 ℃下可储藏25 d,–80 ℃下可分别储藏21和18 d。–80 ℃储藏前干燥处理2 h的花粉,储藏后花粉活力显著高于干燥处理4 h和6 h的花粉。预冻处理显著降低了花粉活力。–80 ℃储藏的花粉采用35 ℃解冻2 min或冰上解冻效果较好。 相似文献
13.
台湾青枣不同品种花粉萌发和生活力测定 总被引：12，自引：0，他引：12
以5个台湾青枣品种盛花期花粉为试材，应用正交试验测定不同品种花粉萌发率及适宜的萌发条件，并用4种不同方法测定花粉生活力。结果表明：（1）不同品种的花粉萌发率存在显著差异，其中大世界的花粉萌发率最高。（2）硼酸浓度、培养温度等因素对花粉萌发率有极显著影响。最适培养基为含琼脂1％、蔗糖15％、硼酸0．02％的组合，适宜温度为30—32℃。（3）花粉生活力的测定中，离体萌发法是最佳选择，I—KI染色法、醋酸洋红染色法和TTC染色法不适宜用作台湾青枣花粉生活力的测定。 相似文献
14.
15.
N. Sukhvibul V. Vithanage M. K. Smith V. J. Doogan Suzan E. Hetherington 《The Journal of Horticultural Science and Biotechnology》2013,88(2):214-222
SummaryThe effect of a constant (10, 15, 20 or 25°C) or a diurnal maximum/minimum (15/5, 20/10, 25/15 or 30/20°C) incubation temperature on in vitro pollen germination and pollen tube growth in the pistils of two poly-embryonic (‘Kensington’ and ‘Nam Dok Mai’), and two mono-embryonic (‘Irwin’ and ‘Sensation’) mango cultivars was studied. In in vitro experiments where pollen was incubated in a liquid germination medium for 24 h in darkness, little difference was found between pollen germination of mono- and poly-embryonic cultivars. Averaged over the four cultivars, 53.9% of pollen germinated at 10°C, this increased to 76.2% when the incubation temperature was increased to 15°C, thereafter up to 25°C the percentage germination remained stable but germination decreased slightly to 68.2% at 30°C. Similarly, there was no difference in percent germination between cultivars when pollen was incubated under diurnal temperature regimes. Mean pollen germination of all four cultivars was 52.3% at 15/5°C and pollen germination increased by 10% when the temperature was raised to 30/20°C. When self-pollinated flowers were incubated for 24 h on a semi-solid agar medium at 10°C, pollen tube growth of the four cultivars was retarded and no pollen tubes reached the ovaries. As the temperature was increased from 15 to 25°C, the mean number of pollen tubes in ovaries increased from 0.04 to 0.25. At 30°C, the mean number of pollen tubes that entered ovaries decreased to 0.04. After incubation under diurnal temperature regimes, the mean number of pollen tubes in ovaries of all four cultivars at 15/5°C was 0.23 and increased to 0.42 when the temperature increased to 30/20°C. At each incubation temperature, there were significant differences in pollen tube growth between cultivars, but there were no differences between the temperature response of pollen from mono- and poly-embryonic cultivars. 相似文献
16.
17.
18.
An improved in vitro pollen germination method was developed for avocado (Persea americana Mill.). The effect of different concentrations of sucrose, polyethylene glycol (PEG), Mg and Ca on pollen germination was evaluated in order to determine the optimal pollen germination medium, i.e. that maximizing the percentage of pollen germination and minimizing the percentage of bursted pollen grains. Once the germination medium was optimized we used it to study the effect of temperature on in vitro pollen germination and tube growth in different cultivars from the three botanical varieties of avocado, that differ in their adaptation to environmental conditions. Significant differences in percentage of pollen germination and in pollen tube growth were observed among cultivars. These results could have implications not only for optimizing pollen management in avocado but also to select the best pollinizers for a particular cultivar. 相似文献