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1.
Dose‐splitting or reduced dose repeat application, i.e. the application of a herbicide twice to the same plant, is likely to become a more common scenario in future, if farmers adopt the use of novel techniques for prediction of herbicide efficacy shortly after application. Fourteen pot experiments were conducted to study whether graminicides applied to annual grass weeds as dose‐splitting treatments were as effective as single applications. The influence of time interval between applications and the ratio of the doses of the two applications were studied. Two statistical approaches were applied: comparison of the ED90 of the single treatments and a joint action model. The study revealed that flupyrsulfuron, iodosulfuron, sulfosulfuron, clodinafop and glyphosate could be applied as repeat treatments with up to 14 days’ interval between the two applications without loss of activity. In some experiments, particularly with iodosulfuron, dose‐splitting enhanced herbicide performance. By contrast, prosulfocarb responded adversely, most likely due to the pronounced impact of weed growth stage on the activity of this herbicide. The ratio of the doses and the timing between the two applications had no significant influence on herbicide responses. The results of the present studies showed that even if a reduced dose of a graminicide results in an unsatisfactory control and it is necessary to re‐spray, the overall use of herbicide will not increase, if methods are available that can predict the efficacy of a herbicide application within the first 1–2 weeks after application.  相似文献   

2.
BACKGROUND: Lolium rigidum Gaud. is one of the most common weed species in winter cereals in north‐eastern Spain, with populations that have evolved resistance to herbicides becoming more widespread since the mid‐1990s. Nine trials on commercial fields with herbicide‐resistant L. rigidum were conducted during the cropping seasons 2001–2002 to 2003–2004, testing the efficacy of 20 herbicides and mixtures pre‐ and post‐emergence and as sequential applications. Weed populations chosen had different resistance patterns to chlortoluron, chlorsulfuron, diclofop‐methyl and tralkoxydim, representative of the resistance problems faced by farmers. RESULTS: In pre‐emergence, prosulfocarb mixed with trifluralin, chlortoluron or triasulfuron was effective on six populations. In post‐emergence, iodosulfuron alone or mixed with mesosulfuron gave the best results but did not control three resistant populations. At Ferran 1, none of the herbicide combinations reached 90% efficacy. CONCLUSIONS: The diverse efficacy patterns of the different populations demonstrate the need for detailed knowledge of the populations before using herbicides. Moreover, the unexpected insufficient efficacy of the new herbicide iodosulfuron prior to its field use shows the need to combine herbicides with other non‐chemical weed control methods to control resistant L. rigidum in north‐eastern Spain. Copyright © 2010 Society of Chemical Industry  相似文献   

3.
Japanese foxtail is one of the most common and troublesome weeds infesting cereal and oilseed rape fields in China. Repeated use during the last three decades of the ACCase-inhibiting herbicide fenoxaprop-P-ethyl to control this weed has resulted in the occurrence of resistance. Dose–response tests established that a population (AHFD-1) from eastern China had evolved high-level resistance to fenoxaprop-P-ethyl. Based on the resistance index, this resistant population of A. japonicus is 60.31-fold resistant to fenoxaprop-P-ethyl. Subsequently, only a tryptophan to cysteine substitution was identified to confer resistance to fenoxaprop-P-ethyl in this resistant population. ACCase activity tests further confirmed this substitution was linked to resistance. This is the first report of the occurrence of Trp-2027-Cys substitution of ACCase in A. japonicus. From whole-plant pot dose–response tests, we confirmed that this population conferred resistance to other APP herbicides, including clodinafop-propargyl, fluazifop-P-butyl, quizalofop-P-ethyl, haloxyfop-R-methyl, cyhalofop-butyl, metamifop, DEN herbicide pinoxaden, but not to CHD herbicides clethodim, sethoxydim. There was also no resistance observed to ALS-inhibiting herbicides sulfosulfuron, mesosulfuron-methyl, flucarbazone-sodium, pyroxsulam, Triazine herbicide prometryne and glyphosate. However, this resistant population was likely to confer slightly (or no) resistant to Urea herbicides chlortoluron and isoproturon.  相似文献   

4.
A. GUR  Y. GIL  B. BRAVDO 《Weed Research》1979,19(2):109-116
Application of several herbicides and a combination of herbicides in two vineyards resulted in a perfect control of Bermuda grass by glyphosate and mixed or alternate applications of MSMA and dalapon. Johnson grass was eliminated by MSM A with and without the addition of dalapon. Dalapon combined with MSMA reduced the yield of the grapevines and their growth, as measured by the weight of pruning. Yields were also significantly reduced by aminotriazole and in one vineyard also by simazine. Reduced vegetative growth was caused by MSMA and glyphosphate. MSMA and glyphosphate reduced the chlorophyll content of the grape leaves and the reducing sugar content of the canes in winter. Amino triazole reduced the reducing sugar content of both leaves and canes and the nitrogen content of the canes. In one vineyard simazine reduced the chlorophyll content of the leaves and increased the nitrogen content of the canes. MSMA alone and in combination with dalapon increased the arsenic content of leaves, canes and fruits of grapevines. Arsenic levels were the highest in the canes, but still did not exceed 0.6 ppm. Levels were lowest in fruits and did not exceed 0.1 ppm. Dalapon reduced the chlorophyll content of leaves in June and increased it in July. It also affected the reducing sugar content of leaves and canes without affecting yields or growth.  相似文献   

5.
Glyphosate-based herbicides (e.g. Roundup Ultra 360 SL) are extensively used in aquatic environment. Although glyphosate is more environmental favorable than many other herbicides, it may be exceptionally dangerous for aquatic ecosystems through high water solubility. Thus, the aim of the work was quantification of influence of Roundup Ultra 360 SL (containing isopropylamine salt of glyphosate as an active ingredient) on biomass and chlorophyll content within duckweed (Lemna minor L.). Moreover, changes in polyamine content and activity of such antioxidative enzymes as catalase (CAT) and ascorbate peroxidase (APX) were assayed in order to determine the biochemical mechanisms of L. minor response to the herbicide treatment. Obtained results showed that phytotoxicity of the herbicide was connected with decrease in chlorophyll-a, b and a+b content, and reduction of biomass growth. Roundup, similarly to some abiotic and biotic stressors, caused over-accumulation of putrescine, spermidine and total polyamines (PAs) within duckweed tissues. In addition an increase in CAT and APX activities suggested that stress generated by the herbicide treatment was at least partially connected with oxidative burst. Intensity of the duckweed responses to the herbicide was dependent on the applied herbicide level and/or duration of treatment.  相似文献   

6.
Cocker  Coleman  Blair  Clarke  & Moss 《Weed Research》2000,40(4):323-334
Aryloxyphenoxypropionate (APP) and cyclohexanedione (CHD) herbicides are used extensively in the UK to control grass weeds, including Avena spp. (wild-oats). Reports of resistance to APP and CHD herbicides are a particular concern for the agricultural community. In this study, the responses of four UK Avena populations were characterized towards the APP herbicides fenoxaprop-P-ethyl and fluazifop-P-butyl, and towards the CHD herbicides cycloxydim and tralkoxydim. An A. sterilis ssp. ludoviciana population (T/41) was found to be highly resistant to fenoxaprop-P-ethyl and fluazifop-P-butyl, but did not show cross-resistance to cycloxydim and tralkoxydim. In contrast, one A. sterilis ssp. ludoviciana (T/11) and one A. fatua population (Dorset) showed partial resistance to both APP herbicides and also showed cross-resistance to the CHD herbicide tralkoxydim, but not to cycloxydim. Before this study, the biochemical mechanisms that confer resistance to the APP and CHD herbicides in UK Avena populations were unknown. Results from the present study show that an enhanced rate of metabolism of fenoxaprop-P-ethyl was found to confer resistance in the two partially resistant Avena populations (T/11 and Dorset), and the presence of an insensitive form of the target enzyme, ACCase, was responsible for target site resistance to fenoxaprop-P-ethyl and fluazifop-P-butyl in the highly resistant population T/41. Cross-resistance to the CHD herbicide tralkoxydim in the T/11 and Dorset populations was not conferred by insensitive ACCase, and was most probably caused by enhanced metabolism. This is the first report that resistance to fenoxaprop-P-ethyl can be conferred by enhanced metabolism in Avena spp.  相似文献   

7.
Eleven free amino acids were identified in the grass weed loose silky-bent (Apera spica-venti) after exposure to four rates of the thiocarbamate herbicide prosulfocarb. A new HPTLC method was developed for quantification of amino acids in raw plant extracts. A pattern of significant changes in the content of glutamine, glycine, alanine, and proline due to exposure were detected. The content of all four amino acids increased at increasing exposure rates. Quantitative analyses 7 and 21 days after herbicide application showed that over time the amount of proline increased, the alanine and glycine content decreased, and that at high exposure rates the amount of glutamine increased. Visual effects were evaluated and the biomass was measured. The visual symptoms increased and the biomass decreased with increasing exposure rates. A significant correlation between the changes in the content of the four amino acids and the visual effects and the biomasses were found.  相似文献   

8.
This study investigated the physiological causes of differences in phytotoxic symptoms shown in barnyardgrass from foliar applications of the herbicides fenoxaprop-P-ethyl and cyhalofop-butyl. When these were applied to the third leaves of the whole plant, the chlorosis and desiccation in the third leaf was greater in fenoxaprop-P-ethyl than cyhalofop-butyl. However, initial growth inhibition of the fourth leaf was greater when using cyhalofop-butyl than when using fenoxaprop-P-ethyl. In the shoot regrowth test, regrowth at five days after treatment (DAT) was smaller in cyhalofop-butyl than in fenoxaprop-P-ethyl; the regrowth at 10 DAT exhibited the reverse trend. The chlorosis (decrease of chlorophylls: carotenoids ratio) in barnyardgrass leaf segments that were floated on herbicide solution was greater in the fenoxaprop-P-ethyl treatment. These results indicate that different herbicidal responses induced by the two herbicides are likely to be related to differential translocation and metabolism. The relatively light chlorosis and desiccation in treated leaves, severe cessation of initial growth (but a lower final herbicidal efficacy in the cyhalofop-butyl treatment) are probably related to its rapid translocation to the meristem region from the treated leaf, followed by faster metabolism. In contrast, the relatively greater chlorosis and desiccation compared to inhibition of initial growth in the fenoxaprop-P-ethyl treatment is likely to be related to its relatively slower translocation and metabolism in the treated leaf.  相似文献   

9.
The phytotoxicity of glyphosate(N-(phosphonomethyl glycine) to seedlings of white mustard (Sinapis alba) cultivated indoors was studied. Yellowing and wrinkling of leaves was observed, necrotic spots appeared and the elongation of the seedlings was significantly reduced at doses 0–49 kg ai/ha and above. Only when sprayed at 4–97 kg ai/ha was the effect of glyphosate 100% lethal (5–7 days after spraying) At the highest concentration of herbicide a marked decrease in chlorophyll content was found but with 0–49 kg ai/ha the chlorophyll content was found to be higher than that in the leaves of control plants. Two and fourteen days after spraying with glyphosate and the commercial product samples of leaf and stem were harvested for electron microscopy. Cellular defects in the leaves ranging from slight swelling to complete disruption of the chloroplasts were detected at the two highest herbicide doses 48 h after spraying. These defects were intensified with time und in addition other sub-morphological changes occurred: decrease in starch grain content, an increase in the number of dictyosomes and mitochondria, disruption of tonoplasts and increase of plastoglobuli In the more central parts of stem segments the commercial product resulted in greater cellular effects than did glyphosate. It is suggested that the differences may be due to the surfactant.  相似文献   

10.
BACKGROUND: Grass seed crops are minor crops that cannot support the development of selective herbicides for grass weed control in grass seed crops. An option is to screen for selective herbicides with the use of logarithmic spraying technology. The aim of this paper is to assess selectivity of various herbicides in grass seed crops by using dose–response curves. RESULTS: Six grass species were subjected to logarithmic spraying with 11 herbicides and with Poa pratensis L. as a weed. The ratio between the doses that caused 10% of damage to the crop and 90% of damage to the weed was used as a selectivity index. Compounds with selectivity indices above 2 can be safely used in a crop. The two ACCase herbicides clodinafop‐propargyl and fenoxaprop‐P‐ethyl and a mixture of the two ALS herbicides mesosulfuron and iodosulfuron could be used selectively to control P. pratensis in Festuca rubra L., although the selectivity indices in no instances were greater than the desired 2.0. CONCLUSION: The logarithmic sprayer can be a rapid screening tool for identifying compounds with favourable selectivity indices. Good experimental design is needed to alleviate rates being systematically distributed and confounded with growth rate and soil fertility gradients. Copyright © 2009 Society of Chemical Industry  相似文献   

11.
Widely used herbicides sometimes inadvertently contaminate surface waters. In this study we evaluate the toxicity of herbicides to aquatic plants and algae and relate it to environmental herbicide concentrations and exposure scenarios, herbicide formulation and mode of action. This was done experimentally for ten herbicides, using the aquatic macrophyte Lemna minor L. and the green alga Pseudokirchneriella subcapitata (Korshikov) Hindak, supplemented with a database study comprising algae toxicity data for 146 herbicides. The laboratory study showed that herbicide formulations in general did not enhance herbicide efficacy in the aquatic environment. The Roundup formulation of glyphosate proved to be the only exception, decreasing the EC(50) of the technical product for both L. minor and P. subcapitata approximately fourfold. Comparison of the sensitivity of L. minor and P. subcapitata revealed up to 1000-fold higher sensitivity of L. minor for the herbicides categorized as weak acids (pK(a) < 5), emphasizing the importance of higher plants in hazard assessment. Database analyses showed that no herbicide group, categorized by site of action, was significantly more toxic than another. Synthetic auxins were the exception as they are virtually non-toxic to unicellular algae. There was no strong correlation between toxicity to algae and K(ow) of the herbicides, not even within groups having the same site of action. Evaluating all data, few herbicides were toxic at concentrations below 1 microg l(-1), which is the 99.9th percentile of the herbicide concentrations measured in the Danish surveillance programme. Joint action of several herbicides cannot however be excluded.  相似文献   

12.
为探讨除草剂与安全剂混用对杂草的防治效果及对糜子安全性的影响,以'榆糜2号'为试验材料,在糜子三叶期选取两种茎叶型除草剂——阔世玛(3.6％ 二磺 ? 甲碘隆水分散粒剂)和锐超麦(20％ 双氟 ? 氟氯酯可湿性粉剂)与两种安全剂——芸苔素内酯(brassinolide,BR)和赤霉素(gibbrellic acid,G...  相似文献   

13.
Glyphosate has been associated with control failures for Spermacoce verticillata in some Brazilian States. The objective of this work was to evaluate and determine the possible causes of this and propose alternative herbicides to use. Glyphosate was applied at three plant stages of development (2–4 leaves, 4–6 leaves and full bloom) where foliar absorption and translocation of glyphosate to various plants parts and time were analysed using the 14C technique. Data were submitted to nonlinear regressions and analysis of variance, where means were compared by a Tukey test at 5% probability. Plant control by the application of different herbicides (19) in the same three stages of development of weed was evaluated. Twenty‐one days after herbicide application, control was visually evaluated and data analysed and means were compared. Due to absorption and/or translocation problems, S. verticillata plants were not controlled by glyphosate. Plants with 4–6 leaves showed lower absorption and translocation of the herbicide to the leaf/root regions compared with plants at the beginning of their development. Plants at full bloom showed lower translocation of the herbicide to the root. In addition to the application of glyphosate at early stages of development, the application of paraquat, flumioxazin and mixtures of glyphosate with flumioxazin or cloransulam is recommended. Late applications could result in poor control, principally if glyphosate is used. Therefore, early applications are strongly recommended for control of this species.  相似文献   

14.
BACKGROUND: The herbicide glyphosate inhibits the biosynthesis of aromatic amino acids by blocking the shikimate pathway. Imazethapyr and chlorsulfuron are two herbicides that act by inhibiting branched‐chain amino acid biosynthesis. These herbicides stimulate secondary metabolism derived from the aromatic amino acids. The aim of this study was to test if they cause any cross‐effect in the amino acid content and if they have similar effects on the shikimate pathway. RESULTS: The herbicides inhibiting two different amino acid biosynthesis pathways showed a common pattern in general content of free amino acids. There was a general increase in total free amino acid content, with a transient decrease in the proportion of amino acids whose pathways were specifically inhibited. Afterwards, an increase in these inhibited amino acids was detected; this was probably related to proteolysis. All herbicides caused quinate accumulation. Exogenous application of quinate arrested growth, decreased net photosynthesis and stomatal conductance and was ultimately lethal, similarly to glyphosate and imazethapyr. CONCLUSIONS: Quinate accumulation was a common effect of the two different classes of herbicide. Moreover, exogenous quinate application had phytotoxic effects, showing that this plant metabolite can trigger the toxic effects of the herbicides. This ability to mimic the herbicide effects suggests a possible link between the mode of action of these herbicides and the potential role of quinate as a natural herbicide. Copyright © 2009 Society of Chemical Industry  相似文献   

15.
Echinochloa colona is the most common grass weed of summer fallows in the grain‐cropping systems of the subtropical region of Australia. Glyphosate is the most commonly used herbicide for summer grass control in fallows in this region. The world's first population of glyphosate‐resistant E. colona was confirmed in Australia in 2007 and, since then, >70 populations have been confirmed to be resistant in the subtropical region. The efficacy of alternative herbicides on glyphosate‐susceptible populations was evaluated in three field experiments and on both glyphosate‐susceptible and glyphosate‐resistant populations in two pot experiments. The treatments were knockdown and pre‐emergence herbicides that were applied as a single application (alone or in a mixture) or as part of a sequential application to weeds at different growth stages. Glyphosate at 720 g ai ha?1 provided good control of small glyphosate‐susceptible plants (pre‐ to early tillering), but was not always effective on larger susceptible plants. Paraquat was effective and the most reliable when applied at 500 g ai ha?1 on small plants, irrespective of the glyphosate resistance status. The sequential application of glyphosate followed by paraquat provided 96–100% control across all experiments, irrespective of the growth stage, and the addition of metolachlor and metolachlor + atrazine to glyphosate or paraquat significantly reduced subsequent emergence. Herbicide treatments have been identified that provide excellent control of small E. colona plants, irrespective of their glyphosate resistance status. These tactics of knockdown herbicides, sequential applications and pre‐emergence herbicides should be incorporated into an integrated weed management strategy in order to greatly improve E. colona control, reduce seed production by the sprayed survivors and to minimize the risk of the further development of glyphosate resistance.  相似文献   

16.
The effects of the phenylurea cytokinin 4PU-30 and the herbicide glyphosate, applied alone and in combination on young maize plants were investigated. The influence of the compounds on the changes of growth, chlorophyll content, levels of hydrogen peroxide, and some stress markers, the activities of peroxidase, catalase, and glutathione-S-transferase, as well as glutathione amount were measured 3, 6, and 10 days after the treatment. The application of glyphosate increased the levels of lipid peroxidation, glutathione, and free proline content, ion fluxes, and the activity of catalase, guaiacol peroxidase, and glutathione-S-transferase, i.e., along with the inhibition of its target enzyme the herbicide induced also an oxidative stress. We found that the phenylurea cytokinin 4PU-30 alleviated in some extent the detrimental effects due to the glyphosate action. Moreover, we speculated that the cytokinin renders its protective action by induction of “hardiness” in the antioxidant defense systems in maize plants similarly to the effects observed after the application of some herbicide safeners.  相似文献   

17.
Glyphosate and glufosinate-ammonium herbicide tolerance traits were combined into both winter and spring lines of Brassica napus L. This allowed the study of possible interactions between these transgenes in two genetic backgrounds when treated with a variety of herbicides. Selective herbicides that are commonly used within Brassica crops showed no adverse effects on the transgenic plants or their null controls. Lines containing both glyphosate and glufosinate transgenes remained tolerant to their respective herbicides, regardless of the presence of the second tolerance transgene. Lines containing only a single transgene retained tolerance to the encoded trait and did not show cross-tolerance to the second. Null lines were killed by either herbicide. All plant lines, regardless of their transgene content, were found to be equally susceptible to three herbicides (paraquat, metsulfuronmethyl and mecoprop), commonly used to remove volunteer B napus from succeeding crops and set-a-side land.  相似文献   

18.
In the intensely farmed corn-growing regions of the mid-western USA, surface waters have often been contaminated by herbicides, principally as a result of rainfall runoff occurring shortly after application of these to corn and other crops. In some vulnerable watersheds, water quality criteria for chronic human exposure through drinking water are occasionally exceeded. We selected three settings representative of vulnerable corn-region watersheds, and used the PRZM-EXAMS model with the Index Reservoir scenario to predict corn herbicide concentrations in the reservoirs as a function of herbicide properties and use pattern, site characteristics and weather in the watersheds. We compared herbicide application scenarios, including broadcast surface pre-plant atrazine and alachlor applications with a glyphosate pre-plant application, scenarios in which losses of herbicides were mitigated by incorporation or banding, and scenarios in which only glyphosate or glufosinate post-emergent herbicides were used with corn genetically modified to be resistant to them. In the absence of drift, in almost all years a single runoff event dominates the input into the reservoir. As a result, annual average pesticide concentrations are highly correlated with annual maximum daily values. The modeled concentrations were generally higher than those derived from monitoring data, even for no-drift model scenarios. Because of their lower post-emergent application rates and greater soil sorptivity, glyphosate and glufosinate loads in runoff were generally one-fifth to one-tenth those of atrazine and alachlor. These model results indicate that the replacement of pre-emergent corn herbicides with the post-emergent herbicides allowed by genetic modification of crops would dramatically reduce herbicide concentrations in vulnerable watersheds. Given the significantly lower chronic mammalian toxicity of these compounds, and their vulnerability to breakdown in the drinking water treatment process, risks to human populations through drinking water would also be reduced.  相似文献   

19.
Lolium rigidum (annual or rigid ryegrass) is a widespread annual weed in cropping systems of southern Australia, and herbicide resistance in L. rigidum is a common problem in this region. In 2010, a random survey was conducted across the grain belt of Western Australia to determine the frequency of herbicide‐resistant L. rigidum populations and to compare this with the results of previous surveys in 1998 and 2003. During the survey, 466 cropping fields were visited, with a total of 362 L. rigidum populations collected. Screening of these populations with the herbicides commonly used for control of L. rigidum revealed that resistance to the ACCase‐ and ALS‐inhibiting herbicides was common, with 96% of populations having plants resistant to the ACCase herbicide diclofop‐methyl and 98% having plants resistant to the ALS herbicide sulfometuron. Resistance to another ACCase herbicide, clethodim, is increasing, with 65% of populations now containing resistant plants. Resistance to other herbicide modes of action was significantly lower, with 27% of populations containing plants with resistance to the pre‐emergent herbicide trifluralin, and glyphosate, atrazine and paraquat providing good control of most of the populations screened in this survey. Ninety five per cent of L. rigidum populations contained plants with resistance to at least two herbicide modes of action. These results demonstrate that resistance levels have increased dramatically for the ACCase‐ and ALS‐inhibiting herbicides since the last survey in 2003 (>95% vs. 70–90%); therefore, the use of a wide range of integrated weed management options are required to sustain these cropping systems in the future.  相似文献   

20.
紫茎泽兰化学防除试验   总被引：1，自引：0，他引：1  
为探究低毒、低残留、高效的药剂防除紫茎泽兰的方法,选用50%特丁赛草隆、75%苄.草苷、41%草甘膦异丙胺盐、50%草甘膦等4种药剂,在紫茎泽兰生长期和非生长期实施叶片正反面喷施试验。结果表明,以41%草甘膦异丙胺盐水剂、50%草甘膦可湿性粉剂效果较好。41%草甘膦异丙胺盐水剂150 mL＋水125 00 g（浓度为1.16%）株防效达100%,50%草甘膦按50 g＋洗衣粉10 g＋水12 500 g株防效达75.33%。同种药剂生长期较非生长期防除效果好,41%草甘膦异丙胺盐水剂在生长期浓度达1.16%时株防效达100%,而在非生长期浓度为1.6%时株防效仅达62.6%。  相似文献   

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