共查询到18条相似文献，搜索用时 203 毫秒
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《中国家禽》2004,26(1):42-42
用人工诱发与自然病例作研究对象,经X线胫骨摄片确定OP诊断后,选择有代表性的30例,按诊断结果分成5组:OP轻度阳性( )14例,中度阳性( )4例,重度阳性( )6例,OP可疑(±)3例,阴性(-)对照3例。取胫骨中段骨组织作超薄切片行透射电镜检查对比,以观察两者的相关性。结果3例X线OP阴性鸡电镜所见为正常,骨细胞和哈佛氏管等超微结构无异常改变。X线OP可疑组和各阳性( 、 、 )组,每组均检出病毒粒子,其大小形态与超微结构都与C型肿瘤病毒相似,为放射学诊断提供了病原证据。X线诊断为OP可疑和各组阳性的病例电镜观察全部有超微结构上的改变… 相似文献
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鸡骨型白血病免疫琼扩与放射摄片诊断的比较 总被引：1，自引：1，他引：0
为了探索特异性免疫学诊断方法对鸡骨型白血病(OP)的检测效果。用双盲法分批采样同时进行羽髓琼脂扩散试验和胫骨X线摄片检查对比,共检834只鸡(433羽属天然自发OP鸡群,401羽属人工诱发实验鸡群)。结果羽髓琼扩试验的OP阳性检出率为3．48％(29／834：)。阳性病理符合率为75．86％(22／29)。阳性误诊(假阳性)率24．13％(7／29),阴性误诊(假阴性)率36．15％(291／805)。而X线摄片检查的OP阳性检出率为38．37％(320／834)。X线阳性完全可获病理组织学证实,不存在假阳性或假阴性。把禽白血病羽髓琼扩试验试用于检测鸡骨型白血病尚属初次报道,其阳性检出率低而误诊率高,尚不宜作OP检测手段。放射摄片诊断的阳性检出率高而误诊率低,可快速诊断,故适用于OP检疫。 相似文献
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近年来,鸡马立克氏病在我县大面积发生和流行,青县畜牧局分别彩临床检查病理剖检、羽毛囊琼扩和血清琼扩进行诊断。现将结果报告如下：１典型临床病例诊断对５０例有明显临床症状（采食减少,消瘦,拉稀,发育不良和／或瘫痪、劈叉）和典型剖检病变（肝、脾、肾、心、肺。肠、卵巢或睾丸）临床诊断为鸡马立克氏病阳性（阳性率１００％）的病例,于剖检前分别采集新生羽毛髓和血清,进行羽毛囊琼扩和血清琼扩试验,结果为：羽毛囊琼扩阳性４１例,阳性率８２％;血清琼扩阳性２２例,阳性率４４％。结果表明,临床诊断为马立克阳性的鸡,羽… 相似文献
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犬瘟热琼脂扩散试验方法的建立及初步应用 总被引：7，自引：0，他引：7
采用犬瘟热鸡胚成纤维细胞弱毒疫苗免疫绵羊制备琼脂扩散抗体，应用犬瘟热病貉肝脏（电镜检查“＋＋”以上）制备琼脂扩散抗原，建立了琼脂扩散试验方法，用于犬瘟热病死动物肝、脾脏器中病毒抗原成分的检出。检测送检病料１０份，与电镜检查结果比较，阳性符合率为８５．７％（６／７），阴性符合率为７５％（３／４），总体符合率为９０％（９／１０）。本方法特异、敏感，操作简便，易于推广应用。 相似文献
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鸡骨型白血病疫情的放射学研究 总被引：5，自引：2，他引：3
报道了鸡骨型白血病在３个城市的１０几个大型鸡场中存在的重大疫情。应用放射学诊断技术，分２０批对３３２８只鸡胫骨组合Ｘ线片进行了观察。被检鸡包括蛋用种的柯比特、海兰和肉用种的ＡＡ、艾维因和ＡＳＡ等１０几个品种，检出该病发生率平均高达３８．２８％（１２７４／３３２８），蛋用鸡群最高者达４８．０２％（９７／２０２），肉用种最高者达７４．０１％（１３１／１７７）。揭示出该病存在的广泛性、高发性和亚临床性。放射学诊断技术可用于该病的检出，对该病检疫和净化可能有重要作用。 相似文献
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狄高辛标记DNA探针对鸡马立克氏病的流行病学研究 总被引：2，自引：0，他引：2
用狄高辛标记的Ⅰ型马立克氏病病毒特异性ＤＮＡ探针，对自然感染鸡羽囊、人工感染鸡羽囊和脏器、同居感染鸡羽囊进行了检测。结果表明，自然感染鸡，其羽囊最早出现阳性的日龄为４２日龄，随日龄增大，羽囊阳性检出率升高，１５０日龄达最高峰（２６．７％）。以后则阳性率显著下降（６月龄为０．４８％，１年以上为０．２４％）。鸡人工感染马立克氏病国际标准强毒（ＭＤＶ－ＧＡ强毒）后３ｄ，就可从５只鸡中３只的心、肝、肺、肾、法氏囊、胸腺、肌胃等脏器检出Ⅰ型ＭＤＶ－ＤＮＡ；２５ｄ，５只鸡的脏器均能检出ＭＤＶ－ＤＮＡ；同时，感染后１０ｄ，就可从羽囊检出ＭＤＶ－ＤＮＡ，１５ｄ羽囊检出阳性率达１００％。同居感染鸡从羽囊排毒的最早时间为同居后３１ｄ，即接触强毒２１ｄ后开始持续排毒，直至死亡。 相似文献
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白兴武 《青海畜牧兽医杂志》2010,40(1):25-26
在被检的934头奶牛中,初检检出阳性结核病牛8头,可疑牛5头,对可疑牛复检后,检出阳性牛1头,共检出阳性牛9头,阳性率为0．96％（9／934）：阳性牛中黑白花奶牛7头,阳性率为1．17％（7／599）,当地黄牛2头,阳性率为0．60％（2／335）;从检测结果看,恰卜恰镇黑白花奶牛的阳性率;比当地黄牛的阳性率高,差异性极显著（P〈0．01）,说明黑白花奶牛对结核病的抵抗力比当地黄牛相对较差。 相似文献
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本文在国内首次报告用骨型白血病患鸡抗凝全血接种 10日龄鸡胚绒毛尿囊腔 ,诱发本病获得成功 ,经X线摄片检查 ,检出发病率为 4 1 3% (19/ 4 6 ) ,其中重度阳性率为 4 2 11% (8/ 19) ,可早在 2 1日龄X线检出病变 ,其中最早有2例于 15日龄临床上已表现出症状。同时用患鸡血腹腔接种 1日龄雏鸡 ,同样诱发出本病 ,X线摄片检查发病率为36 79% (39/ 10 6 ) ,其中重度阳性率为 10 2 6 % (4/ 39)。与腹腔接种法相比 ,两种接种方式所获阳性率无显著差异 (P >0 .0 5 ) ,但重度阳性率差异显著 (P <0 .0 5 )。对人工诱发病鸡作禽白血病特异性血清学诊断 ,其中羽髓琼扩试验 (AGP)检出阳性率为 4 4 9% (4/ 89) ,酶联免疫吸附试验 (ELISA)为首次试用于OP ,检出阳性率为 12 3% (15 / 12 2 )。另对 12 0例生产鸡群老龄鸡进行诊断性普查 ,放射学阳性检出率为 34 17% (41/ 12 0 ) ,AGP试验阳性检出率 1 6 7% (2 / 12 0 ) ,ELISA试验阳性检出率 4 17% (5 / 12 0 )。表明血清学的AGP与ELISA法对OP不敏感 ,可致能反映OP病变存在的X线阳性鸡大量漏诊。 相似文献
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2013年3月,甘肃省某鸡场50日龄鸡发生疑似马立克氏病（MD）,导致大批死亡。为进行进一步确诊和病理学研究,分别采集样品进行琼脂扩散试验和病理组织学观察。结果显示,琼脂扩散试验呈马立克氏病病毒（MDV）抗原阳性。病理学观察显示,病鸡肝、脾、肾、心等实质器官程度不等肿大,色泽变淡,甚至形成大小不等和数量不一的灰白色结节;部分病鸡坐骨神经呈单侧性不规则肿粗,弹性降低或丧失;病鸡实质器官及坐骨神经组织中均出现大量多形态的类似淋巴细胞、成淋巴细胞、浆细胞、巨噬细胞及网状细胞聚集或散在,并可见典型的MD细胞,尤其在血管周围和淋巴管周围;各型肿瘤细胞和MD细胞异型性明显,肿瘤细胞周围实质程度不等变性、坏死,间质水肿。研究证明,该鸡场鸡病为MDV感染引起的马立克氏病（MD）,病理变化主要为高异型性的淋巴样肿瘤细胞的广泛浸润,肿瘤细胞恶性程度高、增生活跃。 相似文献
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Investigations of the occurrence of Avibacterium paragallinarum infections in Uganda 总被引：1，自引：0，他引：1
Byarugaba DK Minga UM Gwakisa PS Katunguka-Rwakishaya E Bisgaard M Olsen JE 《Avian diseases》2007,51(2):534-539
Investigations were conducted to determine the occurrence of Avibacterium paragallinarum in poultry in Uganda. A total of 710 each of bacteriologic and serum samples were taken from chickens and turkeys for demonstration of A. paragallinarum and antibodies. Samples for isolation of A. paragallinarum were also subjected to direct polymerase chain reaction (PCR) for demonstration of the organism's presence. Antibodies to A. paragallinarum were demonstrated in the sera using the hemagglutination inhibition test. A total of five isolates were recovered from two out of five commercial layer chicken farms investigated where suspected cases of infectious coryza were reported, and all of them belonged to Page's serovar C. PCR detected more positive samples (11/68) than did culture (5/68). Isolates were not recovered from free-range poultry nor were there any positive samples by PCR. The overall seroprevalence was 40.5% and the seroprevalence to serovars A, B, and C were 18%, 0.5%, and 22%, respectively. Antibodies to all Page's serovars A, B, and C were demonstrated in free-range chickens but only serovar C antibodies were demonstrated in commercial chickens. No antibodies were demonstrated in turkeys. This is the first time infectious coryza has been confirmed in Uganda and the causative agent, A. paragallinarum, isolated. A high seroprevalence observed in free-range chickens seems to indicate a subclinical infection under extensive village management conditions. 相似文献
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A comparison of gel diffusion, fluorescent antibody and virus isolation methods in experimental and natural cases of infectious bursal disease. 总被引：1，自引：1，他引：0 
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下载免费PDF全文 P R Ide 《Canadian journal of veterinary research》1975,39(2):183-190
In studies with chicks inoculated with the Sk-1 strain of infectious bursal agent the bursa of Fabricius was found to be the tissue of choice for virus isolation as well as for use in the fluorescent antibody test and the agar gel diffusion test. In separate experiments positive results were obtained until postinoculation days 3 or 4 by the agar gel diffusion test, 5 or 6 by the fluorescent antibody test and 14 by the virus isolation method, respectively. Bursas from chickens involved in seven natural outbreaks of infectious bursal disease were then examined by these three methods. Virus was isolated from six outbreaks and infectious bursal agent antigen was demonstrated in three by the agar gel diffusion test method and seven (three by direct examination and four after one passage in chicks) by the fluorescent antibody test method. Passage in chicks was required when nonspecific fluorescence complicated the interpretation of fluorescent antibody test results. 相似文献
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F Goll 《Avian diseases》1989,33(4):760-763
A national survey was conducted to address the concern over the uniformity of serological test results for avian mycoplasmosis. The National Veterinary Services Laboratories produced chicken and turkey mycoplasma serum-check test kits. Each kit had a total of 25 sera that consisted of negative and positive sera. Participating laboratories were requested to examine their kits using the serum plate agglutination test with the plate antigens currently being used and the hemagglutination inhibition test with the hemagglutinating antigens provided. A conclusion whether serum plate agglutination-positive sera were positive, negative, or suspicious was based on the hemagglutination inhibition test results. Results in each category were scored on the basis of 100 points. The average scores on the serum plate agglutination test, hemagglutination inhibition test, and conclusion were 95, 71, and 91, respectively, for the chicken serum test kit and 91, 71, and 89, respectively, for the turkey serum test kit. The results indicated a high degree of uniformity among laboratories in reporting serological test results for mycoplasmosis in chickens and turkeys. 相似文献
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Three groups of 100 individually marked salmonella-free chickens were followed for a period of 53 wk. The chickens were infected as day olds by crop instillation of 10(8) colony-forming units: one group with Salmonella enteritidis and a second group with Salmonella typhimurium. A third group was kept uninfected as controls. The groups were monitored bacteriologically by examination of cloacal swabs and organs and serologically by examination of serum and egg yolk by a lipopolysaccharide enzyme-linked immunosorbent assay throughout the period. Within the first week, 100% of birds in both infected groups were excreting salmonella bacteria in the feces. However, the number of fecal excretors declined rapidly with time, down to 6% in 16 wk for S. typhimurium and down to a similar level within the first 8 wk for S. enteritidis. For the latter, relapses with up to 40% positive birds were observed at the onset of egg production. For both S. typhimurium and S. enteritidis, positive bacteriologic cultures were obtained by sampling from internal organs at the end of the experiment, more than 1 yr from the time of infection. At the age of 6-7 wk, 50% of the chickens in the two infected groups showed a measurable serologic response in serum samples. The response persisted throughout the study in both serum and egg yolk samples. The inclusion of serologic methods is a valuable additional tool in the detection of salmonella in poultry, but serology should be used in conjunction with bacteriologic methods in surveillance programs, in particular to detect flocks in early stages of infection before a measurable serologic response has been raised. 相似文献