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To gain insights into the regulation of fat synthesis, we have investigated the effect of cold environmental exposure and feed restriction of sheep on activity and immunodetectable protein content of acetyl-CoA carboxylase (ACC) and fatty acid synthase in adipose tissue. Subcutaneous and mesenteric adipose tissues were collected at slaughter from sheep exposed to either cold (0+/-2 degrees C) or warm (23+/-2 degrees C) environment, and given either ad libitum or restricted access to feed for three 5-wk periods. Acetyl-CoA carboxylase was isolated from frozen adipose tissue samples and activity determined as the rate of incorporation of H14CO3- into acid stable malonyl-CoA. Cold exposure and feed restriction reduced (P < .05) ACC activity in the two adipose tissue depots. Western blot analysis with peroxidase-conjugated streptavidin showed that both adipose tissue depots express a single isoform of ACC. In s.c. adipose tissue, cold exposure increased (P < .05) ACC protein abundance, which is opposite to the change in activity. However, feed restriction reduced immunodetectable ACC protein. There was no significant effect of environment or feeding level on ACC protein abundance in mesenteric tissue. Fatty acid synthase activity determined in ammonium sulfate extract by measuring the malonyl-CoA- and acetyl-CoA-dependent oxidation of NADPH was decreased (P < .05) by feed restriction in both s.c. and mesenteric tissues. Cold exposure reduced fatty acid synthase activity in s.c. but not in mesenteric tissue. There was no effect of environment on fatty acid synthase protein abundance in either adipose tissue depot. However, feed restriction significantly reduced fatty acid synthase protein abundance in the two depots. The data suggest that feed restriction and exposure of ruminants to cold environmental conditions may significantly down-regulate the activity of key lipogenic enzymes. 相似文献
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The purpose of these studies was to determine the time course for changes in feed intake, blood metabolites, and lipogenic activity in adipose tissue in response to the initiation of porcine somatotropin (pST) treatment and following withdrawal from treatment in barrows. An initial study was conducted to determine the impact of chronic pST treatment (4 wk of daily injection; 0 vs 4 mg/d) on adipose tissue lipid metabolism in barrows (initial weight 67 kg). Feed efficiency was improved 27%, backfat thickness was decreased 43%, and glucose and lactate oxidation and incorporation into lipid in adipose tissue was reduced 70 to 86% in pST-treated pigs. Palmitate esterification was decreased 44%, whereas palmitate oxidation was unaffected. In vitro metabolism of lactate, glucose, and palmitate in liver slices was not affected by pST treatment. The time-course for changes in intake and adipose tissue metabolism in response to 7 d of pST (0 vs 4 mg/d) treatment and 7 d of withdrawal was examined in subsequent studies in barrows (initial weight 75 kg). Feed intake during pST treatment was significantly (P < .05) less than in control pigs within 24 h of the initiation of treatment and remained low through 3 d after withdrawal. Adipose tissue biopsies were obtained on d 0, 1, 2, 4, and 7 of the treatment phase and on d 2, 4, and 7 after withdrawal from 7 d of treatment. Maximal inhibition of lipogenesis by pST treatment in adipose tissue in vitro was observed on d 4 (-68%) and d 7 (-69%). Similarly, fatty acid synthase activity declined during the treatment period, with the greatest change noted on d 7 (-26%). After withdrawal from treatment, lipogenesis gradually increased, returning to control values 7 d after withdrawal. Levels of IGF-I began to increase from d 1 to d 7 of treatment, continually decreased during withdrawal, and were normalized by the end of the withdrawal period. Plasma urea nitrogen concentrations decreased during treatment, increased during the withdrawal phase, and were normalized 4 d after the last pST treatment. Overall results indicate that most of the metabolic changes in response to pST occur within 1 wk of treatment and return to pretreatment values after 7 d of withdrawal from treatment. 相似文献
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The effect of porcine somatotropin (pST) on the lipid profiles of adipose tissue and muscle was investigated. Sixteen crossbred barrows were injected daily with either 3 mg of pST or a placebo. After slaughter, total lipid and fatty acid composition of raw subcutaneous (SC) adipose and intermuscular (IM) adipose tissue and longissimus muscle were determined. The SC adipose tissue from pST-treated pigs had a 7.5% decrease in total lipid content; specific fatty acids 16:0, 18:0, and 18:1(n-9)c decreased most. The IM fat from pST-treated pigs had lower levels of 16:0 and 20:0. There was no effect of pST treatment on the lipid profile of the longissimus muscle. The data suggest that pST treatment produces small but significant changes in the saturated fatty acid content of adipose tissue in pigs. 相似文献
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Our objective was to determine the influence of bovine growth hormone (bGH) and bovine growth hormone-releasing factor (bGRF) administration on the mRNA abundance of lipoprotein lipase (LpL) and stearoyl-CoA desaturase (SCD). Primiparous Holstein cows received bGH, bGRF, or no treatment from 118 to 181+/-1 d postpartum. We hypothesized that bGH and bGRF treatment would increase the mRNA abundance of both SCD and LpL in the mammary gland with a corresponding reduction in adipose tissue. Milk yield significantly increased but milk fat percentage did not change as a result of bGH or bGRF treatment. Short-, medium-, and long-chain fatty acid concentrations in milk were not affected by either bGH or bGRF treatments, with the exception of a modest, but significant, increase in C16:1 and C18:1 following bGH treatment. Analysis was conducted on the genes encoding LpL (E.C. 3.3.1.34), a key enzyme involved in the uptake of fatty acids into tissues, and SCD (E.C. 1.14.99.5), which is the enzyme responsible for introducing delta9 double bonds in fatty acids of 16 and 18 carbons in length. In adipose tissue, treatment with bGH and bGRF reduced the mRNA abundance of LpL to 14.6 and 25.7% respectively, of that observed for control animals. Similarly, these treatments reduced the SCD mRNA abundance to undetectable levels in adipose tissue. In mammary gland, bGH and bGRF had no significant impact on LpL mRNA abundance. Bovine GH did not significantly affect SCD mRNA abundance in the mammary gland, and bGRF reduced SCD mRNA abundance. From this study to examine the role of bGH and bGRF on the expression of the genes encoding these key lipogenic enzymes in cattle, we conclude that the increased substrate required for enhanced milk fatty acid yield may have been provided through redirection of nutrients to the mammary gland away from adipose tissue and through overall increased metabolism in the mammary gland. 相似文献
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Effect of recombinant porcine somatotropin on energy and protein utilization by growing pigs: interaction with capacity for lean tissue growth. 总被引：1，自引：0，他引：1
Three litters of four pigs from each of four different groups were used to evaluate the effect of porcine somatotropin (pST) on growth performance, body gain composition, energy and N metabolism, and in vitro cytochrome oxydase (final enzyme of the respiratory chain) activity of tissues. The four groups included boars from a synthetic line (SG1) or the Large White breed (SG2) and barrows from the Large White breed (SG3) or crossbred between Large White and Meishan breeds (SG4). Inherent capacity for daily lean tissue growth (LTG) decreased from SG1 to SG4. Within a litter, one pig was slaughtered and dissected at the beginning of the experiment (55 kg BW) and the three others were fed the same daily supply of protein and amino acids (26 g of lysine/d) but relative daily energy levels were either 113 (without pST: E1/0), 100 (3 mg of pST/d: E2/pST) or 87 (3 mg of pST/d: E3/pST). The 100 energy level corresponded to the ad libitum intake of E2/pST pigs. Two energy and N balances were carried out in respiration chambers during the experimental period. Pigs were slaughtered and dissected at approximately 95 kg BW and composition of gain was estimated using the comparative slaughter technique. In E1/0 pigs, daily BW, lean, and N gain were affected (P less than .01) by group; 566, 471, 374, and 315 g/d of lean tissue gain in SG1, SG2, SG3, and SG4 pigs, respectively. At high ME intake (E2/pST vs E1/0), pST increased daily BW (+14%), lean (+27%), or N (+26%) gain and reduced adipose tissue (-50%) gain, but the pST effect was inversely related to LTG: for N, the improvement was 2.8, 7.1, 7.0, and 11.1 g/d in SG1, SG2, SG3, and SG4 groups, respectively. Energy restriction (E3/pST vs E2/pST) reduced (P less than .001) adipose tissue gain in all groups but did not affect lean tissue or N gains in SG1, SG2, and SG3 pigs. In the pST-treated pigs of the SG4 group, the lean tissue or N gains were reduced (P less than .01) by energy restriction. Energy restriction combined with pST treatment (E3/pST) led to negligible amounts of fat deposited (40 g/d for SG1 + SG2 + SG3 pigs) and a gain:feed ratio higher than 500 g/kg (580 in SG1 pigs). The increased heat production measured in pST-treated pigs was due to its maintenance component: 275 vs 257 kcal of ME.kg BW-.60.d-1 (P less than .01).(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
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Freese LG Rehfeldt C Fuerbass R Kuhn G Okamura CS Ender K Grant AL Gerrard DE 《Domestic animal endocrinology》2005,29(3):457-475
The aim of this study was to examine whether exogenous somatotropin (ST) can alter the insulin-like growth factor (IGF) axis in the porcine epitheliochorial placenta. Crossbred gilts were injected either 6 mg of recombinant porcine ST or vehicle from days 10 to 27 after artificial insemination (term day 116). Control and ST-treated gilts were euthanized on day 28 (8 control/5 treated), day 37 (4 control/6 treated), and day 62 (4 control/6 treated) of gestation. Endometrium and placental tissue samples were collected and subjected to mRNA analyses. In control gilts, somatotropin receptor (STR) and IGF-I mRNA abundance in the endometrium decreased with gestation. Conversely, the amounts of IGF-II mRNA and of IGF binding protein (BP)-2 and -3 mRNA, which were analyzed in endometrium and placental chorion, increased with gestation. The endometrium contained less IGF-II mRNA but more IGFBP-2 and-3 mRNA than the placental chorion. In response to pST treatment, the amounts of endometrial STR and IGF-I mRNA were lower at days 28 and 37, but higher at day 62 of gestation. The content of IGF-II mRNA was higher in the endometrium of pST-treated than control gilts on day 37. The amount of IGFBP-2 mRNA was increased on day 37 in endometrium and placenta of pST-treated gilts, whereas no changes in IGFBP-3 mRNA were observed. The IGF-II/IGFBP-2 ratio was higher in the placenta in response to pST on day 28 of gestation. Results show that pST treatment of pregnant gilts during early gestation alters IGF axis in maternal and fetal placental tissues and suggest pST may exert an effect on fetal growth by altering the relative amount of IGFBPs and IGFs at the fetal-maternal interface. 相似文献
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t10,c12-CLA对猪皮下脂肪和背最长肌组织脂类代谢的影响 总被引：1，自引：0，他引：1
试验首先建立了30日龄猪皮下脂肪和背最长肌组织块体外培养体系,共设2个处理,处理1为阴性对照(添加0.1mmol/l牛血清白蛋白,BSA),处理2添加100μmol/l的t10,c12-CLA,每个处理设6个平行,接种时即加入BSA与t10,c12-CLA进行处理至第10d,试验结束后收集细胞保存备用。采用荧光定量PCR技术及试剂盒检测研究t10,c12-CLA对猪皮下和背最长肌脂肪代谢关键酶(FAS、ME、LPL、HSL)和激素(INSR、GHR)及甘油三酯(TG)合成的影响。结果表明:(1)100μmol/lt10,c12-CLA显著降低了猪皮下脂肪TG含量并提高了猪背最长肌的TG含量(P0.05);(2)100μmol/lt10,c12-CLA显著抑制了猪皮下脂肪FAS、INSR与背最长肌HSL和LPL的基因表达,促进了猪皮下脂肪HSL和背最长肌INSR的基因表达(P0.05);(3)本研究结果从脂肪代谢关键酶类与调控激素方面揭示了t10,c12-CLA对猪皮下和背最长肌组织脂肪代谢与沉积的差异性调控机制,进一步证实了t10,c12-CLA抑制猪皮下脂肪沉积同时提高肌内脂肪含量。 相似文献
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Dietary conjugated linoleic acid alters fatty acid composition of pig skeletal muscle and fat 总被引：8，自引：0，他引：8
The dietary dose responsiveness of conjugated linoleic acid (CLA) addition relative to the fatty acid profile of edible lean tissue was examined in grower pigs treated with or without porcine somatotropin (pST). Gilts and barrows were fed CLA at 0, 0.25, 0.5, 1.0, or 2.0% of diet by weight from 20 to 55 kg BW. Additional pigs were administered (pST) at 0 or 100 microg x kg BW x d(-1) and fed either 0.5 or 2.0% CLA. Animals were fed diets containing 18% CP, 1.2% lysine, and 3.5 Mcal of DE/kg at 110% of ad libitum intake. The fatty acid profile in latissimus dorsi and dorsal s.c. adipose tissue samples was determined by gas chromatography. Dietary CLA replacement of corn oil increased the percentage of total fatty acids as stearic acid, whereas the percentages as oleic and linolenic acids were reduced in lattisimus muscle. Treatment with CLA + pST increased the percentages of linoleic and arachidonic acids while reducing the percentages of palmitic and oleic acids in lattisimus muscle. Dietary CLA increased the percentages of palmitic and stearic acids in s.c. adipose tissue while reducing the percentages of oleic, linoleic, linolenic, and arachidonic acids. The percentage of palmitic acid was reduced in s.c. adipose tissue, whereas linoleic acid was increased with CLA + pST. No synergistic effect was detected between CLA and pST for reducing carcass lipid content in grower pigs. However, pST increased the percentage of polyunsaturated fatty acids in lattisimus muscle and s.c. adipose tissue while reducing the percentages of saturated fatty acids in swine fed CLA. 相似文献
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Effects of dietary copper on the expression of lipogenic genes and metabolic hormones in steers 总被引：3，自引：0，他引：3
An experiment was conducted to determine the effects of Cu supplementation on performance, subcutaneous adipose tissue mRNA expression of acetyl CoA carboxylase (ACC), stearoyl CoA desaturase (SCD), uncoupling protein 2 (UCP2), and leptin in growing and finishing steers. Forty-eight purebred Angus steers were allotted to one of five treatments: 1) control (no supplemental Cu); 2) 10 mg Cu/kg DM from CuSO4; 3) 10 mg Cu/kg DM from a Cu amino acid complex (Availa Cu); 4) 20 mg Cu/kg DM from CuSO4; 5) 20 mg Cu/kg DM from Availa Cu. Steers were fed an alfalfa hay corn-based diet for 56 d (basal diet contained 7.1 mg Cu/kg DM) and switched to a high-concentrate diet for 144 d (basal diet contained 6.1 mg Cu/kg DM). Blood samples were obtained every 28 d throughout the entire experiment. On d 112 of the finishing period, subcutaneous adipose tissue biopsies were obtained from the tailhead of three animals per treatment and analyzed for ACC, SCD, UCP2, and leptin mRNA expression. Animal performance was not affected by Cu supplementation during the growing phase. Steers receiving 10 mg Cu/kg DM from Availa Cu had higher (P < 0.05) ending body weights and tended (P < 0.10) to have higher ADG than steers receiving 10 mg Cu/kg DM from CuSO4 during the finishing phase. Serum concentrations of nonesterified fatty acid and insulin were not affected by Cu supplementation. Steers receiving supplemental Cu tended (P < 0.11) to have less backfat relative to controls. However, dietary Cu did not influence the level of subcutaneous adipose tissue ACC and SCD mRNA. Neither UCP2 nor leptin gene expression was affected by Cu supplementation. These results indicate that dietary Cu supplementation (10 to 20 mg Cu/kg DM diet) may alter lipid metabolism of subcutaneous adipose tissue; however, it does not seem to affect expression of certain lipogenic genes. 相似文献
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试验首先建立了30日龄猪皮下脂肪和背最长肌组织块体外培养体系,共设2个处理,处理1为阴性对照（添加0.1mmol/L牛血清白蛋白,BSA）,处理2添加100μmol/L的t10,c12-CLA,每个处理设6个平行,接种时即加入BSA与t10,c12-CLA进行处理至第10天,试验结束后收集细胞保存备用。采用荧光定量PCR技术及试剂盒检测研究t10,c12-CLA对猪皮下和背最长肌脂肪代谢关键酶（FAS、ME、LPL、HSL）和激素（INSR、GHR）及甘油三酯（TG）合成的影响。结果表明：①100μmol/Lt10,c12-CLA显著降低了猪皮下脂肪TG含量并提高了猪背最长肌的TG含量（P〈0.05）;②100μmol/Lt10,c12-CLA显著抑制了猪皮下脂肪FAS、INSR与背最长肌HSL和LPL的基因表达,促进了猪皮下脂肪HSL和背最长肌INSR的基因表达（P〈0.05）;③该研究结果从脂肪代谢关键酶类与调控激素方面揭示了t10,c12-CLA对猪皮下和背最长肌组织脂肪代谢与沉积的差异性调控机制,进一步证实了t10,c12-CLA抑制猪皮下脂肪沉积同时提高肌内脂肪含量。 相似文献
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Manipulation of growth in pigs through treatment of the neonate with clenbuterol and somatotropin 总被引：2，自引：0，他引：2
Neonatal pigs were treated with lipolytic agents to determine whether this would cause a long-term decrease in their ability to deposit fat, with a consequent increase in muscle growth and feed efficiency. Groups of 25 female piglets were given clenbuterol (100 microg/kg BW), porcine somatotropin (pST; 100 microg/kg BW), pST plus clenbuterol, or saline injections from 3 d to 40 d of age. Five piglets from each group were then slaughtered to determine body composition. Clenbuterol and pST both increased ADG up to weaning when given separately (24%, P < 0.05; 20%, P < 0.1 respectively) but did not reduce fat deposition. In contrast, pigs given clenbuterol plus pST showed no increase in ADG and a 41% reduction in carcass fat (P < 0.05). Clenbuterol caused a marked decrease in beta2-adrenoceptor density in porcine adipose tissue (P < 0.001) and skeletal muscle (P < 0.01). This effect was attenuated by concurrent pST treatment, which helps to explain the synergistic effect of these drugs on fat deposition. Once the drugs were withdrawn at 40 d, the anabolic effect of pST gradually disappeared, so that the live weight of pST-treated and control pigs was identical at 168 d. Clenbuterol withdrawal caused the rapid loss of extra weight gained, plus an additional 4 to 5 kg live weight that was never recovered. During the 4-wk finishing period there was an increase in feed intake in pigs that had previously undergone treatment with pST (23%, P < 0.1), with no increase in ADG, and so feed efficiency was impaired (P < 0.05). Pigs that were treated with pST plus clenbuterol showed no marked increase in feed intake during this period. Carcasses from clenbuterol-treated pigs tended to be leaner at 168 d, but there was no long-term effect of pST or the combined treatment on carcass composition. Overall, the treatment of neonatal pigs with repartitioning agents was counter-productive, due to the withdrawal effects of the beta-adrenefgic agonist and the delayed long-term effect of pST on feed intake. 相似文献
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Shishuai Sun Qingwei Meng Zhang Luo Baoming Shi Chongpeng Bi Anshan Shan 《Journal of animal physiology and animal nutrition》2019,103(3):813-821
The purpose of this article was to investigate the effects of dietary resveratrol supplementation during gestation and lactation of sows on the milk composition of sows and the fat metabolism of sucking piglets. Forty sows were allotted to two experimental treatment groups that included the following: (a) control sows (CON treatment, n = 20) fed with a corn–soybean meal control diet and (b) treatment sows (RES treatment, n = 20) fed with a control diet with addition of 300 mg/kg resveratrol. The results showed that the content of lactose in the colostrum was increased (p < 0.05) and the content of fat in 21‐day milk was increased (p < 0.05) by dietary resveratrol supplementation. In the RES treatment group, the concentrations of high‐density lipoprotein‐cholesterol (HDL‐C), low‐density lipoprotein‐cholesterol (LDL‐C), lipase activity and insulin (INS) in plasma of sucking piglets were increased (p < 0.05). In the adipose tissue, the enzyme activities of hormone‐sensitive lipase (HSL), acetyl‐CoA carboxylase (ACC) and lipoprotein lipase (LPL) increased significantly by RES treatment (p < 0.05), and the mRNA levels of acetyl coenzyme A‐alpha (ACCα), LPL, fatty acid transport protein (FATP1) and CCAAT–enhancer‐binding protein gene (C/EBPα) were higher in the RES treatment group (p < 0.05). In conclusion, resveratrol supplementation on gestational and lactating sows improved the content of lactose in the colostrum and the content of fat in milk at day 21 of lactation. In addition, resveratrol supplementation on sows increased HDL and LDL in the plasma of piglets. In piglet adipose tissue, the enzyme activity and mRNA level related to lipolysis, fatty acid uptake from circulating triacylglycerols and lipogenesis are partially improved by resveratrol supplementation on sows. These aspects affect fat metabolism in piglets. 相似文献
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为探讨脂联素(ADPN)与激素敏感脂肪酶(HSL)对围产期奶牛脂肪动员的影响及其相互关系,随机选取围产期健康奶牛10头,分别于产前28天、产前14天、产后1天、产后14天和产后28天,应用荧光定量RT-PCR法检测其脂肪组织ADPN mRNA和HSL mRNA丰度。结果表明:在围产期,随着分娩日龄的临近,其脂肪组织的HSL mRNA水平随着ADPN mRNA丰度的降低而下降,产后则随其升高而上升,初步推测脂联素可能是通过调控脂肪HSL mRNA的表达和分泌来调节围产期奶牛脂肪的。 相似文献
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Sadri H Mielenz M Morel I Bruckmaier RM van Dorland HA 《Journal of animal physiology and animal nutrition》2011,95(6):790-797
The objective was to study changes in plasma leptin concentration parallel to changes in the gene expression of lipogenic- and lipolytic-related genes in adipose tissue of dairy cows around parturition. Subcutaneous fat biopsies were taken from 27 dairy cows in week 8 antepartum (a.p.), on day 1 postpartum (p.p.) and in week 5 p.p. Blood samples were assayed for concentrations of leptin and non-esterified fatty acids (NEFA). Subcutaneous adipose tissue was analysed for mRNA abundance by real-time qRT-PCR encoding for leptin, adiponectin receptor 1 (AdipoR1), adiponectin receptor 2 (AdipoR2), hormones-sensitive lipase (HSL), perilipin (PLIN), lipoprotein lipase (LPL), acyl-CoA synthase long-chain family member 1 (ACSL1), acetyl-CoA carboxylase (ACC), fatty acid synthase (FASN) and glycerol-3-phosphate dehydrogenase 2 (GPD2). Body weight and body condition score of the cows were lower after parturition than before parturition. The calculated energy balance was negative in week 1 and 5 p.p., with higher negative energy balance in week 1 p.p. compared with that in week 5 p.p. On day 1 p.p., highest concentrations of NEFA (353.3 μmol/l) were detected compared with the other biopsy time-points (210.6 and 107.7 μmol/l, in week 8 a.p., and week 5 p.p. respectively). Reduced plasma concentrations of leptin during p.p. when compared with a.p. would favour increasing metabolic efficiency and energy conservation for mammary function and reconstitution of body reserves. Lower mRNA abundance of ACC and FASN expression on day 1 p.p. compared with other biopsy time-points suggests an attenuation of fatty acid synthesis in subcutaneous adipose tissue shortly after parturition. Gene expression of AdipoR1, AdipoR2, HSL, PLIN, LPL, ACSL1 and GPD2 was unchanged over time. 相似文献
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Adipose tissue expresses adipokines, which are involved in regulation of energy expenditure, lipid metabolism, and insulin sensitivity. To adapt for the transition from pregnancy to lactation, particularly in high-yielding dairy cows, adipokines, their receptors, and particular G-protein coupled receptors (GPRs) are of potential importance. Signaling by GPR 41 stimulates leptin release via activation by short-chain fatty acids; GPR 43/109A inhibits lipolysis, and GPR 109A thereby mediates the lipid-lowering effects of nicotinic acid and β–hydroxybutyrate. The aim of this study was to compare the mRNA expression of adiponectin and visfatin, adiponectin receptors 1 and 2 (AdipoR1/2), leptin receptor (obRb), insulin receptor as of the aforementioned GPRs during the transition period in high-yielding dairy cows. Biopsies from subcutaneous fat and blood samples were obtained from 10 dairy cows 1 week before and 3 weeks after calving. For AdipoR1 and AdipoR2 mRNA abundance as well as for leptin concentrations in plasma, a reduction (P ≤ .05) was observed postpartum; for visfatin and putative GPR 109A mRNA abundance in adipose tissue, there was a trend (P < .1) for analogous changes. In contrast, the mRNA content of obRb and GPR 41 in adipose tissue was higher (P ≤ .05) in samples from early lactation than in those from late gestation. Our results indicate decreasing adiponectin sensitivity in adipose tissue after calving, which might be involved in the reduced insulin sensitivity of adipose tissue during early lactation. In addition, visfatin, GPR 41, and GPR 109A may further modulate insulin sensitivity. 相似文献
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T. E. Weber B. J. Kerr M. E. Spurlock 《Journal of animal physiology and animal nutrition》2008,92(5):569-577
Soy protein regulates adiponectin and peroxisome proliferator‐activated receptor α (PPARα) in some species, but the effect of dietary soy protein on adiponectin and PPARα in the pig has not been studied. Therefore, the objective of this study was to determine whether soya bean meal reduction or replacement influences serum adiponectin, adiponectin mRNA, serum metabolites and the expression of PPARα and other genes involved in lipid deposition. Thirty‐three pigs (11 pigs per treatment) were subjected to one of three dietary treatments: (i) reduced crude protein (CP) diet containing soya bean meal (RCP‐Soy), (ii) high CP diet containing soya bean meal (HCP‐Soy) or (iii) high CP diet with corn gluten meal replacing soya bean meal (HCP‐CGM) for 35 days. Dietary treatment had no effect on overall growth performance, feed intake or measures of body composition. There was no effect of dietary treatment on serum adiponectin or leptin. Dietary treatment did not affect the abundance of the mRNAs for adiponectin, PPARα, PPARγ2, lipoprotein lipase or fatty acid synthase in adipose tissue. The mRNA expression of PPARα, PPARγ2, lipoprotein lipase or fatty acid synthetase in loin muscle was not affected by dietary treatment. In liver tissue, the relative abundance of PPARα mRNA was greater (p < 0.05) in pigs fed the HCP‐Soy diets when compared to pigs fed RCP‐Soy or HCP‐CGM diets. Hepatic mRNA expression of acyl‐CoA oxidase or fatty acid synthase was not affected by dietary treatment. Western blot analysis indicated that hepatic PPARα protein levels were decreased (p < 0.05) in pigs fed the RCP‐Soy diets when compared to pigs fed the HCP‐Soy diets. These data suggest that increasing the soy protein content of swine diets increases hepatic expression of PPARα without associated changes in body composition. 相似文献