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1.
球孢白僵菌菌株抗紫外线能力与毒力的关系   总被引:5,自引:0,他引:5  
实验证明紫外光对球孢白僵菌孢子的影响表现为两个方面,一方面导致孢子活率降低,另一方面造成孢子萌发延迟,从而表现为菌株毒力下降。两者和毒力的相关性分别为0.8293及-0.8448,均达极显著水平。  相似文献   

2.
为掌握白僵菌发酵罐培养技术,白僵菌可工业化生产,以宜昌夷陵区白僵菌菌种为材料,对白僵菌液体发酵罐发酵培养的培养基、菌种生长形态、生长环境的pH值进行试验研究,从而确定转罐时间等液体发酵罐发酵技术参数。结果表明:在液体发酵培养中,碳、氮、磷为白僵菌生长必需的三要素,不同生长时期三要素比例不同,初期加入少量微量元素有助于提高白僵菌孢子生长速率和增加数量;在白僵菌液体发酵生长史中,初始pH值为6,继而在pH值4~5的环境中生长速率最快;前42~45 h在种子罐中主要产菌丝体,在转罐后21~26 h发酵罐中主要产孢子体。  相似文献   

3.
实验证明紫外光对球孢白僵菌孢子的影响表现在两个方面,一方面导致孢子活率降低,另一方面造成孢子萌发延迟,从而表现为菌株毒力下降。两者和毒力的相关性分别为0.8293及-0.8448,均达极显著水平。  相似文献   

4.
<正> 利用白僵菌防治松毛虫,在我国南方几省是一种主要手段。但在生产技术上仍是采用土法固体发酵生产分生孢子,此法原材料消耗大,杂菌污染影响菌粉质量和操作人员健康。黑龙江省曾用液体发酵生产白僵菌芽生孢子,此法工艺简单,节省原材料,但芽生孢子不耐贮存。苏联于七十年代初采用一步法液体深层培养生产出分生孢子。为克服土法生产中的缺点。我们于1984—1986年开展了液体深层发酵生产分生孢子的研究工作,现将结果报告如下。  相似文献   

5.
浙江省白僵菌生产推广应用技术   总被引:1,自引:0,他引:1  
浙东白僵菌生产从70年代初的常规固体浅盘发酵到80年代的开放性平板培养发酵、机械化提取白僵菌孢子粉生产工艺,生产技术日益提高,生产规模不断扩大,产品质量持续。稳定。1970~1991年,全省生产白僵菌899.69t,平均年产40.89t,产品含孢量108亿个/g,活孢率79%,含水量8.5%,各项指标基本符合省定标准。在温州、丽水、台州等10个地(市)的66个县(市)推广应用,防治松毛虫142.0  相似文献   

6.
通过观察、测定6株毒力不同的球孢白僵菌菌株在SDAY培养基平板上的萌发行为及萌发参数发现,白僵菌萌发孢子具有几种萌发形态,单芽管萌发型、双芽管萌发型、三芽管萌发型及拟附着孢型。对马尾松毛虫幼虫毒力的高低,与孢子萌发中时(GT50)相关极显著(r=0.9240),与单、双芽管孢子比例(C1/C2)(r=0.8598)、及双芽管孢子的长芽管与短芽管平均长度的差值(X1-X2)(r=0.8376)均相关显著。菌株毒力与萌芽管平均长度及萌芽管整齐度(CV)相关不显著。  相似文献   

7.
冯玉元 《林业调查规划》2004,29(2):99-101,104
用大米做培养基生产白僵菌,经多次实验,成功地从大米培养基产品上得到了比较纯净的白僵菌孢子粉产品,即白僵菌纯孢产品。其每克纯孢含3000多亿个孢子,比常规白僵菌产品含100亿个孢子的质量显著高出。成本低廉,有利于包装和运输。  相似文献   

8.
用白僵菌防治马尾松毛虫是目前普遍推广使用的一种方法,具有防治费用低,使用方便,不污染环境等特点。当前生产白僵菌主要采用三级发酵培养用此方法生产的白僵菌原粉体积大,含水量高,有效保存期一般只有半年,如不及时使用就会变质失效,造成浪费。根据这一情况我们试制了“GF——80型白僵菌孢子吸附粉碎机”。该机既能粉碎白僵菌原粉,  相似文献   

9.
从受侵染的美国白蛾蛹体内分离出白僵菌菌落,并对该白僵菌菌落进行纯化,获得白僵菌纯菌落。采用生长速率法和悬滴法研究了不同湿度、紫外线、营养液和不同培养基对球孢白僵菌Bb01菌株营养生长和孢子萌发的影响。结果表明,球孢白僵菌Bb01菌丝生长和孢子萌发的最适温度为25℃,在此温度下,15h孢子萌发率在100%;在湿度为100%的条件下菌丝生长良好,孢子萌发率高;经紫外线照射18h孢子萌发率在0%~17.00%范围内,而对菌丝生长影响不大。  相似文献   

10.
使用4种球孢白僵菌(Beauveria bassiana)在室内对美国白蛾5龄幼虫进行了防治试验。试验结果表明,白僵菌菌株Bb11对美国白蛾有较高的侵染效果,同时明确了其致死中浓度(LC50)。田间试验表明,浓度为5×108孢子/mL的白僵菌防治效果为89.8%,浓度为1×109孢子/mL的白僵菌防治效果为94.2%,浓度为2×109孢子/mL的白僵菌防治效果为96.9%。因此,在使用过程中应保持一定的孢子浓度以保障防治效果。  相似文献   

11.

In Scandinavia, moose (Alces alces L.) sometimes cause severe browsing damage to economically-important pine. Moose-vehicle accidents have spurred construction of fences along roads, and these may interfere with moose migration between summer and winter ranges, or the road alone may be a barrier. If this happens and moose build up along roads, landowners may suffer economically. Therefore, this study investigated whether roads, fences or other factors influence the use of young pine stands by moose. Eighty stands along roads in northern Sweden were evaluated in which individually-browsed branches were counted on 9972 pines. Moose browsing was not significantly related to birch (Betula pendula Roth, B. pubescens Ehrh.) density, nor did it differ between pines (Pinus contorta Douglas or P. sylvestris L.). However, increased pine density, site productivity and proximity to a highway were associated with increased browsing. Further large-scale studies are needed to understand moose habitat selection and the effects of roads.  相似文献   

12.

The root systems of 2-yr-old Picea glauca, Picea mariana and Pinus banksiana seedlings were submitted to various frost temperatures during an artificial frost to induce different levels of root damage. Frost-damaged and control seedlings were placed in a greenhouse under high and low soil moisture regimes. Seedling growth and physiology were evaluated periodically. Seedling survival was reduced when root damage reached levels of 60-80%. Root systems of all three species showed partial to total recovery by the end of the experiment. In general, root freezing damage caused reductions in seedling growth, with these reductions becoming less significant over time. Root damage had little to no effect on black spruce and jack pine seedling physiology, while white spruce CO 2 uptake decreased with increasing root damage. Shoot nitrogen content of all three species decreased slightly with increasing root damage.  相似文献   

13.

This study investigated the stand structure in pine, spruce and deciduous forests in the border district of Finland and Russia. A total of 46 mature forest stands was selected as pairs, the members of each pair being as similar as possible with respect to their forest site type, age, moisture and topography. The stands were then compared between the two countries by means of basal areas and number of stems. The proportions of dominating tree species were 2-12% lower, and correspondingly the proportions of secondary tree species higher, in Russian forests. The density of the forest stock was also higher in each forest type in Russia. The forests in the two countries differed most radically in terms of the abundance of dead trees. The amount was two to four times higher in Russian deciduous and spruce forests, and in pine forests the difference was 10-fold. The stand structures indicated that Russian coniferous stands, in particular, were more heterogeneous than intensively managed pine and spruce stands in Finland.  相似文献   

14.

The root collar diameter and the height:diameter ratio are of particular importance in container-grown seedlings where a high density in the containers may produce spindly seedlings. Temperature regimes and light quality are known to affect plant growth. The aim of this study was to identify responses in Picea abies (L.) Karst. seedlings grown with light providing different red:far-red ratios and under temperature regimes with alternating day (DT) and night temperature (NT) from negative (DT < NT) to positive (DT > NT) difference (DIF) between DT and NT. Experiments were conducted in controlled environment chambers and in a daylight phytotron. Only limited thermoperiodic responses appeared in P . abies seedlings with respect to seedling height and dry weight accumulation. The formation of terminal buds, however, was clearly delayed in seedlings grown at negative DIF. The results indicate a requirement for day extension light that is high in far-red, to prevent terminal bud formation under natural short-day conditions. An extended study should be conducted to clarify the minimum level of light intensity and the optimal light quality needed to prevent terminal bud formation under natural short-day conditions.  相似文献   

15.
本文分析了CAD在设计中引起的正面和负面影响,并进行了系统的阐述,从而使设计者在应用中能够保持客观的态度。  相似文献   

16.

The effects of soil scarification (mounding), slash removal and clear-cut age on the natural regeneration in clear-cuts was evaluated using data from four sites in southern Sweden. The treatments were carried out during a good seed and establishment year for birch ( Betula pubescens Ehrh. and B. pendula Roth). Scarification had the strongest positive effect on the density of naturally regenerated seedlings, especially in birch, but also in pine ( Pinus sylvestris L.) and spruce [ Picea abies (L.) Karst]. Slash removal had a positive effect on birch density. No statistically significant effect of clear-cut age was found. In addition, no statistically significant interactions between clear-cut age and scarification or slash removal were found. The ingrowth of field vegetation was the fastest in areas that were not scarified, less rapid in areas scarified on old clear-cuts, and the slowest after scarification in fresh clear-cuts. In conclusion, it may be possible to control the density of birch during a good establishment year for birch. If birch is desired, the best combination of treatments is to remove the slash and scarify; otherwise, these treatments should be avoided.  相似文献   

17.
18.

Based on an enquiry, risk perception among non-industrial private forest owners is described in relation to climate change and forestry hazards. Of the respondents, 11% took action to remedy the effects of climate change. Out of a given set, hazards were ranked according to each respondent's experience of recent substantial financial loss to the estate and in relation to his or her willingness to make investments aimed at risk reduction. For each hazard, the respondent assessed the risk in four classes ranging from very high to negligible risk. Six hazards were considered most problematic in all three aspects: browsing damage, falling timber prices, damage by wind, spruce bark beetle, root rot and pine weevil. A majority of the respondents claimed to take action to reduce the risk associated with at least one hazard, while 35% did not know whether they did. Excluding climate change, the need for decision support was the largest in relation to damage by wind owing to a combination of perceived high risk and a high level of ignorance in relation to whether risk-reducing measures were taken.  相似文献   

19.
In situ produced plant residues contain a mixture of different plant components of varying quality. To assess synergistic or antagonistic interactions occurring during the decomposition and mineralization of such mixtures, individual plant parts (stems, leaves, leaf litter and roots) or the mixture of stems, leaves and leaf litter of the agroforestry species pigeonpea (Cajanus cajan) or of crop residues of peanut (Arachis hypogaea) or of the weed hairy indigo (Indigofera hirsuta) were incubated in pots for 19 weeks. Periodically, remaining plant residues were sieved out (>2 mm), weighed and N content as well as soil mineral N determined. Above- and below-ground residues of peanut decomposed fastest and showed the largest N release in agreement with their high N concentration and low-acid detergent fibre (ADF) : N ratio. Hairy indigo was hypothesized to be of lower quality than pigeonpea because of its high-polyphenol content. However, it decomposed faster than pigeonpea, largely because of the higher N and lower lignin concentration of its components. Ranking of individual plant components for N mineralization resulted in the following pattern, leaves > leaf litter > roots > stems. In mixtures of the different plant components a similar species order in decomposition was obtained, e.g. peanut > hairy indigo > pigeonpea. The amount of N released from the mixture was dominated by stem material that comprised 46–61% of the mixture. The interactions in mixtures were relatively small for peanut (generally high-quality components) as well as for pigeonpea (low proportion of high-quality components, i.e. N rich leaf material). However, a positive interaction occurred during later stages of N mineralization in the mixture of hairy indigo as it had a significant proportion of N rich components and absence of highly reactive polyphenols. Thus, for plants with low to intermediate chemical quality attributes, manipulating plant composition (e.g. by varying harvest age, affecting stem and leaf proportions) will be important to obtain significant interactions during decomposition when its components are mixed.  相似文献   

20.
The use of organic waste materials such as milk sewage as an organic fertilizer could have the dual advantages of organic-waste disposal and reduced dependence on inorganic fertilizers. The effects of fertilization with (1) conventional mineral fertilization, (2) milk sewage sludge at 40 kg N ha−1 target rate and (3) no fertilization on pasture production and tree growth were examined in an experiment consisting of two pasture mixtures under a one-year-old Pinus radiata plantation with a density of 2500 trees ha−1. The two pasture mixtures were: (1) Dactylis glomerata L. var. saborto (25 kg ha−1) + Trifolium repens L. group Ladino (4 kg ha−1) + Trifolium pratense L. var. Marino (1 kg ha−1); (2) Lolium perenne L. var. Tove (25 kg ha−1) + Trifolium repens L. group Ladino (4 kg ha−1) + Trifolium pratense L. var. Marino (1 kg ha−1). The experiment began in the spring of 1995 using a randomized block design with three replicates in Castro Riberas de Lea (Lugo, Galicia, north-western Spain). Plot size was 12 × 8 m2, with a 1 m buffer strip between plots. Two-year data showed that fertilization with either material had a positive effect on pasture production, with no significant difference between the two fertilization treatments. Tree growth in the milk sewage sludge plot was significantly higher than in the control plots. Inorganic fertilization increased pasture production, but affected tree growth negatively. The results show that milk sewage sludge could be used as a fertilizer in silvo-pastoral systems. This revised version was published online in June 2006 with corrections to the Cover Date.  相似文献   

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