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1.
采用微波辅助提取豆粕中大豆异黄酮,通过单因素实验和正交实验研究了微波火力、乙醇浓度、料液比、微波时间对大豆异黄酮提取率的影响.结果表明,在试验条件范围内各因素对大豆异黄酮提取率的影响主次顺序为:微波火力>乙醇浓度>料液比>微波时间.微波辅助提取大豆异黄酮的最佳条件为:料液比1∶20(g∶mL),微波火力中高火,微波时间3 min,乙醇浓度50%.在此条件下,大豆异黄酮的提取率可达到1.2432%.  相似文献   

2.
以黑豆为原料,按照一定条件萌发处理。利用微波辅助提取法,通过对料液比、乙醇浓度、微波处理时间、微波功率进行单因素和正交试验,探讨黑豆豆芽中大豆异黄酮提取率的影响因素,并优化筛选其最佳提取工艺条件。结果表明:影响大豆异黄酮提取率的因素顺序为:微波处理时间料液比微波功率乙醇浓度。黑豆豆芽中大豆异黄酮的最佳微波提取工艺为:料液比1∶25、乙醇浓度50%、微波处理4 min、微波功率为450 W。在此条件下,黑豆豆芽中大豆异黄酮的提取率为0.595%。  相似文献   

3.
为优化提取和纯化低温豆粕中皂甙的加工工艺,采用微波法提取低温豆粕中的大豆皂甙,通过单因素试验和正交试验研究了微波火力、乙醇浓度、料液比、微波时间对大豆皂甙提取率的影响.结果表明:在试验条件范围内各因素对大豆皂甙提取率影响的主次顺序为微波时间>微波火力>乙醇浓度>料液比;在微波火力为中高火、微波时间2.5 min、乙醇浓度60%、料液比1:25(g:mL)的条件下得到最优工艺条件,此时大豆皂甙的提取率为0.640%.  相似文献   

4.
以茶籽为原料,以茶籽皂苷提取得率为考察指标,系统研究了乙醇浓度、微波强度、微波辅助提取时间、料液比等因素对微波辅助提取茶籽皂苷的得率影响,优化筛选了微波辅助提取茶籽皂苷的最优工艺技术及其参数.结果表明,采用乙醇体积分数60%、微波功率539 W、微波辅助提取时间7 min、料液比1∶50的工艺参数进行微波辅助提取茶籽皂苷,皂苷得率可达18.56%.与常规水提取、乙醇提取相比,微波辅助提取的茶籽皂苷得率分别提高了6.84%、6.22%,且提取时间大为缩短.  相似文献   

5.
李雅晶  吴建阳  郑雅婷 《大豆科学》2012,31(1):112-114,118
通过正交实验考察了微波提取温度、微波提取时间、料液比、提取次数对大豆皂苷得率的影响,并研究了大豆皂苷对酪氨酸酶活性的影响。结果表明:微波辅助提取大豆皂苷的较佳工艺为:微波处理时间30 min,微波处理温度40℃,料液比1∶5,提取3次。在上述条件下测得大豆皂苷得率8.11%;大豆皂苷对酪氨酸酶活性具有抑制作用,且随浓度的增加,抑制作用增强,其IC50值为222.7μg.mL-1。  相似文献   

6.
龙牙楤木总皂苷提取工艺的优化   总被引:1,自引:0,他引:1  
目的确定龙牙楤木总皂苷的最佳提取工艺。方法采用正交试验比较超声提取法、微波提取法和热回流浸提法提取龙牙楤木总皂苷的工艺及效率。结果超声法的最佳提取工艺为:料液比1:30、80%乙醇、时间60min,皂苷得率为7.56﹪;微波法的最佳提取工艺为:料液比1:25、80%乙醇、时间50s、功率6400w,皂苷得率为7.32﹪;热回流法的最佳提取工艺为:料液比1:25、时间2.5h、60%乙醇、温度60℃,皂苷得率为7.19﹪;结论超声提取法优于微波提取法和回流提取法。  相似文献   

7.
大豆与大豆芽中异黄酮提取工艺研究   总被引:1,自引:0,他引:1  
姚占静  郭睿  李宁 《大豆科学》2008,27(2):357-360
以乙醇为溶剂,分别以大豆、大豆芽为原料,研究了异黄酮的提取工艺,并在最佳工艺条件下探讨了两者中异黄酮的得率,试图寻找提取大豆异黄酮的最佳原料和工艺方法.结果表明温度、乙醇浓度、料液比、提取时间等'因素对异黄酮的提取均产生不同程度的影响,大豆芽中异黄酮含量比大豆中高.通过正交试验得出从大豆、大豆芽中提取异黄酮的最佳工艺条件分别为温度80℃,乙醇浓度70%,料液比120,时间2 h;温度80℃,乙醇浓度70%,料液比114,芽长6 mm,在此条件下异黄酮的提取率分别为1854、2105 μg·g-1.  相似文献   

8.
于海莲 《大豆科学》2011,30(1):144-146
以乙醇作为萃取剂,采用微波法从大豆中提取异黄酮,分别考察了微波功率、乙醇的体积分数、料液比和微波辐射时间对异黄酮提取量的影响.结果表明:从大豆中提取异黄酮的最佳工艺条件:微波功率400 w、乙醇的体积分数50%、料液比1:4、微波辐射时间5 min.在最佳工艺条件下,异黄酮的提取量1.3205 mg.g-1.与传统溶剂...  相似文献   

9.
以茶籽为原料,以茶籽皂苷提取得率为考察指标,系统研究了乙醇浓度、微波强度、微波辅助提取时间、料液比等因素对微波辅助提取茶籽皂苷的得率影响,优化筛选了微波辅助提取茶籽皂苷的最优工艺技术及其参数。结果表明,采用乙醇体积分数60%、微波功率539 W、微波辅助提取时间7 min、料液比1:50的工艺参数进行微波辅助提取茶籽皂苷,皂苷得率可达18.56%。与常规水提取、乙醇提取相比,微波辅助提取的茶籽皂苷得率分别提高了6.84%、6.22%,且提取时间大为缩短。  相似文献   

10.
响应面法优化枇杷花总黄酮超声波辅助提取工艺的研究   总被引:3,自引:0,他引:3  
采用超声波辅助提取枇杷花中的总黄酮,研究超声波功率、乙醇浓度、提取温度、提取时间、液料比及提取次数对总黄酮提取得率的影响。根据单因素试验,选择在超声波功率80 W、一次浸提条件下优化提取工艺;采用Box-Behnken中心组合试验设计原理,设计4因素3水平试验,以响应面分析法优化乙醇浓度、提取温度、提取时间及液料比4个因素对总黄酮提取得率的影响。结果表明:当超声波功率为80 W时,枇杷花中总黄酮超声波辅助提取的优化工艺参数为:乙醇浓度47.33%、提取温度58.42 ℃、提取时间12.01 min、液料比(mL ∶ g)45.56 ∶ 1,在此条件下枇杷花总黄酮一次浸提的提取得率可达10.48%。  相似文献   

11.
12.
Summary

The efficiency of N fertilizers is usually poor; often less than 50% of the applied N is taken up by the crop. This review focuses on various N fertilizers with respect to the significance of different N loss pathways, namely (i) ammonia volatilization, (ii) dinitrogen and nitrogen oxide emissions, and (iii) nitrate leaching. Further, the significance of biological N immobilization, ammonium fixation and, finally, the impact of nitrate vs. ammonium uptake on crop yield are also discussed. The reviewed literature shows that N fertilizers may differ markedly in their susceptibility to losses. There is, however, considerable scope to improve N efficiency of each N source by proper N management practices.  相似文献   

13.
Summary The effects of the leaves of five plant species, one from each of the generaAmbrosia, Anemone, Eupatorium, Eucalyptus andLantana, on potato tuber moth were investigated under indigenous storage conditions at the Central Potato Research Station, Shillong (1800 m above sea level). Their action was compared with that of a biological insecticide (spores ofBacillus thuringiensis), a chemical insecticide (carbaryl), and an untreated control. The data collected after six months storage on tuber damage, sprout damage and the rotting indicated that the leaves ofLantana aculeata provided most protection to the tubers, reducing damage from over 70% in the check to below 5%, and sprout damage from over 45% to below 3%. Next best wasEucalyptus globulus followed byB. thuringiensis. They may be used on tubers stored for table use or for seed as they had no adverse effect on germination or on the yield of a subsequent crop.  相似文献   

14.
15.
Novel food and non-food uses for sorghum and millets   总被引:4,自引:3,他引:4  
Sorghum and millets have considerable potential in foods and beverages. As they are gluten-free they are suitable for coeliacs. Sorghum is also a potentially important source of nutraceuticals such antioxidant phenolics and cholesterol-lowering waxes. Cakes, cookies, pasta, a parboiled rice-like product and snack foods have been successfully produced from sorghum and, in some cases, millets. Wheat-free sorghum or millet bread remains the main challenge. Additives such as native and pre-gelatinised starches, hydrocolloids, fat, egg and rye pentosans improve bread quality. However, specific volumes are lower than those for wheat bread or gluten-free breads based on pure starches, and in many cases, breads tend to stale faster. Lager and stout beers with sorghum are brewed commercially. Sorghum's high-starch gelatinisation temperature and low beta-amylase activity remain problems with regard to complete substitution of barley malt with sorghum malt . The role of the sorghum endosperm matrix protein and cell wall components in limiting extract is a research focus. Brewing with millets is still at an experimental stage. Sorghum could be important for bioethanol and other bio-industrial products. Bioethanol research has focused on improving the economics of the process through cultivar selection, method development for low-quality grain and pre-processing to recover valuable by-products. Potential by-products such as the kafirin prolamin proteins and the pericarp wax have potential as bioplastic films and coatings for foods, primarily due to their hydrophobicity.  相似文献   

16.
Summary The in vitro antimicrobial activity of extracts from accessions ofSolanum commersonii Dun. collected in the south of Uruguay was investigated against five microorganisms including the pathogenRalstonia solanacearum. A total of 30 extracts corresponding to organic and aqueous extracts were studied. Interestingly, most of the positive results for growth inhibition were againstR. solanacearum. The extracts were also analyzed for the presence of glycoalkaloids and lectins. Six of the ten aqueous extracts showed lectin presence and a wide variation in the type and amounts of glycoalkaloids, was found. Results indicate that there is no clear relationship between the antimicrobial activity against the five microorganisms screened and the presence or amounts of lectins and glycoalkaloids, traditionally regarded as possible antimicrobial metabolites in theSolanum genus, which indicates the presence of as yet unidentified antimicrobial compounds.  相似文献   

17.
Summary Clones derived from thirty-one different accessions (nineteen of Argentine origin) belonging to eightSolanum species were screened for resistance to infection by potato virus X strain cp (PVX cp) by mechanical inoculation of plantlets that had been micropropagated in vitro. Estimates of PVX multiplication obtained by enzyme linked immunosorbent assay and slot blot nucleic acid hybridization allowed the identification of resistant clones derived from five accessions belonging toS. commersonii S. oplocense, S. sparsipilum andS. tuberosum andigena. Resistant genotypes supported PVX concentrations 5 to 15 times smaller than did the susceptible control cultivar Spunta. Graft inoculation test confirmed the presence of extreme resistance similar to that conferred by the ‘immunity’ gene X1 (also called RXact).  相似文献   

18.
The loss of density and elasticity, the appearance of wrinkles and hyperpigmentation are among the first noticeable signs of skin aging. Beyond UV radiation and oxidative stress, matrix metalloproteinases (MMPs) assume a preponderant role in the process, since their deregulation results in the degradation of most extracellular matrix components. In this survey, four cyanobacteria strains were explored for their capacity to produce secondary metabolites with biotechnological potential for use in anti-aging formulations. Leptolyngbya boryana LEGE 15486 and Cephalothrix lacustris LEGE 15493 from freshwater ecosystems, and Leptolyngbya cf. ectocarpi LEGE 11479 and Nodosilinea nodulosa LEGE 06104 from marine habitats were sequentially extracted with acetone and water, and extracts were analyzed for their toxicity in cell lines with key roles in the skin context (HaCAT, 3T3L1, and hCMEC). The non-toxic extracts were chemically characterized in terms of proteins, carotenoids, phenols, and chlorophyll a, and their anti-aging potential was explored through their ability to scavenge the physiological free radical superoxide anion radical (O2•−), to reduce the activity of the MMPs elastase and hyaluronidase, to inhibit tyrosinase and thus avoid melanin production, and to block UV-B radiation (sun protection factor, SPF). Leptolyngbya species stood out for anti-aging purposes: L. boryana LEGE 15486 presented a remarkable SPF of 19 (at 200 µg/mL), being among the best species regarding O2•− scavenging, (IC50 = 99.50 µg/mL) and also being able to inhibit tyrosinase (IC25 = 784 µg/mL), proving to be promising against UV-induced skin-aging; L. ectocarpi LEGE 11479 was more efficient in inhibiting MMPs (hyaluronidase, IC50 = 863 µg/mL; elastase, IC50 = 391 µg/mL), thus being the choice to retard dermal density loss. Principal component analysis (PCA) of the data allowed the grouping of extracts into three groups, according to their chemical composition; the correlation of carotenoids and chlorophyll a with MMPs activity (p < 0.01), O2•− scavenging with phenolic compounds (p < 0.01), and phycocyanin and allophycocyanin with SPF, pointing to these compounds in particular as responsible for UV-B blockage. This original survey explores, for the first time, the biotechnological potential of these cyanobacteria strains in the field of skin aging, demonstrating the promising, innovative, and multifactorial nature of these microorganisms.  相似文献   

19.
The peer-reviewed marine pharmacology literature from 2009 to 2011 is presented in this review, following the format used in the 1998–2008 reviews of this series. The pharmacology of structurally-characterized compounds isolated from marine animals, algae, fungi and bacteria is discussed in a comprehensive manner. Antibacterial, antifungal, antiprotozoal, antituberculosis, and antiviral pharmacological activities were reported for 102 marine natural products. Additionally, 60 marine compounds were observed to affect the immune and nervous system as well as possess antidiabetic and anti-inflammatory effects. Finally, 68 marine metabolites were shown to interact with a variety of receptors and molecular targets, and thus will probably contribute to multiple pharmacological classes upon further mechanism of action studies. Marine pharmacology during 2009–2011 remained a global enterprise, with researchers from 35 countries, and the United States, contributing to the preclinical pharmacology of 262 marine compounds which are part of the preclinical pharmaceutical pipeline. Continued pharmacological research with marine natural products will contribute to enhance the marine pharmaceutical clinical pipeline, which in 2013 consisted of 17 marine natural products, analogs or derivatives targeting a limited number of disease categories.  相似文献   

20.
Chitosan is considered to be one of the most promising and applicable materials in adsorption applications. The existence of amino and hydroxyl groups in its molecules contributes to many possible adsorption interactions between chitosan and pollutants (dyes, metals, ions, phenols, pharmaceuticals/drugs, pesticides, herbicides, etc.). These functional groups can help in establishing positions for modification. Based on the learning from previously published works in literature, researchers have achieved a modification of chitosan with a number of different functional groups. This work summarizes the published works of the last three years (2012–2014) regarding the modification reactions of chitosans (grafting, cross-linking, etc.) and their application to adsorption of different environmental pollutants (in liquid-phase).  相似文献   

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