共查询到19条相似文献，搜索用时 734 毫秒
1.
采用软脂酸诱导3T3-L1脂肪细胞胰岛素抵抗后,给予不同剂量的六堡茶水提物治疗,探讨六堡茶对胰岛素抵抗3T3-L1脂肪细胞的糖脂代谢的影响。结果表明,1 mmol·L-1的软脂酸对3T3-L1脂肪细胞作用24 h后,葡萄糖摄取量减少,建立胰岛素抵抗模型。六堡茶对模型细胞干预24 h后,与模型组比较,细胞培养基的残留液中葡萄糖和游离脂肪酸的含量降低;对糖脂代谢相关关键酶（己糖激酶、6-磷酸果糖激酶-1、丙酮酸激酶、甘油-3-磷酸酰基转移酶）和葡萄糖转运子基因表达都有上调作用,并下调乙酰辅酶A羧化酶、蛋白酪氨酸磷酸酯酶1B基因表达。低剂量的六堡茶下调肉碱脂酰转移酶-I基因的表达,但是高剂量能显著上调肉碱脂酰转移酶-I基因表达。实验证明了六堡茶能够提高胰岛素抵抗3T3-L1脂肪细胞对葡萄糖的摄取能力,同时促进细胞内糖脂代谢,六堡茶有改善3T3-L1脂肪细胞胰岛素抵抗的作用。 相似文献
2.
茯砖茶中几种单体成分功效的高通量筛选研究 总被引：4，自引：3，他引：1
选择与降脂减肥作用相关的FXR、LXR、PPARδ、PPARγ及3T3-L1模型对从茯砖茶中分离制备的6个单体化合物进行高通量药物筛选研究。结果表明,各单体对所选模型均有活性。对于FXR激活模型,没食子酸(GA)和表儿茶素没食子酸酯(ECG)在50μg/ml的初筛浓度时激活值分别达1.766和3.220;表没食子儿茶素没食子酸酯(EGCG)在10μg/ml和50μg/ml的初筛浓度时激活作用均较强,特别是50μg/ml的初筛浓度时,其激活倍数达6.000;对于PPARδ模型,此三种单体添加量均需达50μg/ml,作用才显著。在初筛浓度为30μg/ml时,没食子儿茶素(GC)对PPARγ模型的激活倍数达1.619;3-甲氧基-4,5-二羟基苯甲酸(MDBA)对PPARγ模型的激活倍数达1.734;3,4-二羟基苯甲酸(DBA)对FXR抑制值为3.641,明显小于对照初级胆汁酸鹅脱氧胆酸(CDCA)的抑制值6.435,因此其抑制模型的作用非常明显。以上试验结果说明此6个化合物均为活性化合物。 相似文献
3.
探究鸠坑龙井茶水提物（LJT）对小鼠肝组织脂质代谢SREBPs通路信号影响及肠道菌群的调节作用。通过高脂饮食诱导小鼠构建非酒精性脂肪肝（NAFL）模型,并给予LJT(300 mg·kg-1)灌胃干预。定期记录小鼠的体质量,检测小鼠血清生化指标和葡萄糖耐受水平,观察并分析Hematoxylin-Eosin(HE)染色、油红O染色肝组织切片特征;应用Real-time qPCR技术检测小鼠肝组织SREBPs通路7个基因（SREBP-1c、FAS、SCD-1、ACC-1、SREBP-2、HMGCR、PPARγ）的相对表达量,采用蛋白免疫印记技术（Western Blot）分析肝组织蛋白质表达水平,同时对小鼠肠道菌群进行高通量测序（16 SrDNA）并分析其结构。结果显示,LJT干预后小鼠体质量、血糖AUC、血清TG、TC、LDL-C和肝脏中TG、TC水平有显著下降,龙井组小鼠肝组织SREBP-1c、FAS、ACC-1、SCD-1和PPARγ蛋白表达水平降低,SREBP-1c、SCD-1、FAS、ACC-1、SREBP-2、HMGCR、PPARγ基因的相对表达量显著下调;16 S rDNA分析... 相似文献
4.
高通量筛选研究茯砖茶降脂功效 总被引：18，自引：12，他引：6
寻找高效天然的降脂减肥药物是当前研究的热点之一,高通量药物筛选是近年发展起来的药物筛选新方法。将茯砖茶水提液分别用三氯甲烷、乙酸乙酯、正丁醇萃取,制得三氯甲烷层、乙酸乙酯层、正丁醇层及水层样品,茶渣用95%的乙醇提取,均采用过氧化物酶增殖体活化受体(PPARα、PPARγ、PPARδ)、肝X受体(LXR)、法尼酯衍生物X受体(FXR)及前脂肪细胞(3T3-L1)模型进行高通量药物筛选研究茯砖茶的降脂减肥功效,结果显示茯砖茶各部位对PPARγ、PPARδ有激活作用,对FXR有抑制作用,对PPRAα、3T3-L1作用不明显,证明茯砖茶有多条降脂途径,降脂减肥功能较强,有望开发成天然的降脂药物,其降脂有效成分还有待进一步研究。 相似文献
5.
普洱茶功能成分单体降糖降脂作用研究 总被引：10，自引：5，他引：5
本文选取与糖脂代谢相关的PPARδ、PPARα、PPARγ、FXR、LXR、3T3-L1和α-淀粉酶模型,探讨了普洱茶中单体功能成分尿嘧啶、没食子酸的降糖降脂作用。结果表明:尿嘧啶、没食子酸在PPARγ、FXR、LXR模型表现出明显的活性作用,其中尤以没食子酸对PPARγ的激活效果最为显著,其值高达2.438,与阳性对照药物的激动效果相当,而对PPARδ、PPARα、3T3-L1模型的活性作用较弱。在PPARγ模型中,没食子酸对PPARγ受体的激活作用强于尿嘧啶,而在FXR、LXR模型中尿嘧啶的活性作用要强于没食子酸。此外,没食子酸对α-淀粉酶也有较强的抑制作用。本研究结果可为普洱茶的降糖降脂作用机理提供一定的理论依据。 相似文献
6.
目的研究人参皂苷Rh2的促神经分化作用,初步明确相关分子机制。方法将细胞分为对照组、实验组,使用MTT检测Rh2对细胞毒性的影响,筛选最适药物浓度,用倒置相差显微镜观察拍照,使用Image-J软件对照片进行细胞分化率和神经突起长度统计分析,用Western Blotting检测其促神经分化所激活的信号通路,之后用相关通路抑制剂加以干预,检测神经突起长度、分化率和相关通路的蛋白表达变化。结果人参皂苷Rh2浓度在2~6μM不影响细胞存活,能够促进神经分化,随剂量依赖性增加,其中6μM的效果最显著,其通过激活PI3K/Akt、MEK/Erk发挥作用,其中p-Akt和p-Erk在30 min表达最明显,Rh2可以降低由于PI3K/Akt、MEK/Erk抑制剂所致p-Akt、p-Erk抑制的效果。结论人参皂苷Rh2促进神经突起生长、增加和延长,具有较好促进神经分化的效果,具有开发为治疗神经退行性疾病的潜力。 相似文献
7.
脂肪组织类型与人体代谢密切相关,通过饮食或营养干预将白色脂肪细胞转变为产热的米色脂肪细胞是一种减少脂肪积蓄、调节代谢的安全策略。目前关于白色脂肪组织米色化作用的研究多聚焦于肥胖群体,为探究EGCG对非肥胖代谢紊乱群体的内脏白色脂肪组织米色化的诱导作用及相关机制,采用非肥胖型自发性2型糖尿病模型Goto-Kakizaki(GK)大鼠,给予每日高脂饮食,并进行40 mg·kg-1和80 mg·kg-1 EGCG灌胃干预,检测GK大鼠的体质量、摄食量、脂肪组织细胞形态及米色化相关基因表达水平、UCP1蛋白表达水平,并进行转录组测序。结果表明,80 mg·kg-1 EGCG灌胃干预对GK大鼠摄食量和体质量无明显影响,但能够促使脂肪细胞呈现向多房型脂肪细胞转变趋势,并显著上调米色化相关的Pparg、Ppargc1a、Ucp1基因表达水平和UCP1蛋白表达水平,具有诱导高脂饮食GK大鼠内脏附睾白色脂肪组织米色化的作用,且表现出调节脂质代谢的潜力。结合转录组分析结果表明,EGCG对高脂饮食GK大鼠白色脂肪组织米色化的诱导作用机制可能与... 相似文献
8.
为探索EGCG对Nicotine诱导肺癌细胞增殖的抑制作用,本研究通过MTT实验筛选出EGCG、Nicotine对肺腺癌细胞H1299的最佳作用浓度,利用实时荧光定量PCR技术检测EGCG、Nicotine对H1299细胞中Bax、Bcl-2、Jak2和Stat3基因mRNA相对表达量的变化。实验结果表明,EGCG对H1299细胞的半抑制浓度IC50值约为32βμmol·L-1（24βh）、15βμmol·L-1（48βh）;1βμmol·L-1的Nicotine对H1299细胞促增殖作用明显;以15βμmol·L-1的EGCG预处理H1299细胞24βh可显著下调1βμmol·L-1 Nicotine的促增殖作用（P<0.05）。1βμmol·L-1的Nicotine处理H1299细胞可明显降低JAK2/STAT3信号通路中Bax基因mRNA的表达,增加Bcl-2、Jak2、Stat3基因的mRNA表达;15βμmol·L-1 EGCG预处理H1299细胞可反向调控Nicotine诱导所致JAK2/STAT3信号通路中Jak2、Stat3和Bax、Bcl-2基因表达量的变化,结果具有显著性差异（P<0.05）。由此可知,EGCG对Nicotine诱导的肺腺癌H1299细胞增殖及JAK2/STAT3信号通路中促增殖基因mRNA的表达起抑制作用。 相似文献
9.
选用不同浓度的染料木素(GEN)及二十二碳六烯酸(DHA)作用于MCF-7细胞,研究大豆中抗癌成分染料木素与不同比例的多不饱和脂肪酸(PUFAs)联用,对乳腺癌细胞MCF-7增殖及脂代谢基因表达情况的影响。根据DHA的有效浓度设定亚油酸(LA)及LA∶DHA(10∶1)混合PUFAs作用浓度。将GEN分别与DHA、LA及LA∶DHA(10∶1)联合作用细胞,检测细胞生存率,并通过qPCR方法检测细胞内过氧化物酶体增殖物激活受体γ(PPARγ)、5-脂氧合酶(5-LOX)、环氧合酶-2(COX-2)mRNA表达情况。结果表明:高浓度GEN(300,200,100μmol·L~(-1))及DHA(400,300,200μmol·L~(-1))可抑制MCF-7细胞的增殖,且随着时间增加抑制作用增强。而低浓度GEN(50,25μmol·L~(-1))及LA组及10∶1组对细胞增殖有轻度促进作用。添加GEN的G+L组和G+10∶1组细胞的生存率显著降低,同时GEN可降低G+L组细胞5-LOX mRNA的表达量,且GEN与LA∶DHA(10∶1)联用,增加了细胞内PPARγ mRNA的表达水平并抑制了COX-2 mRNA的表达。试验证实GEN可抑制高比例ω-6 PUFAs对乳腺癌细胞增殖的促进作用,其作用机制主要是通过增加PPARγ mRNA表达,抑制COX-2 mRNA表达水平实现的。大豆的抗乳腺癌作用可能是由于GEN与PUFAs在抑制乳腺癌细胞增殖方面产生了协同效应。 相似文献
10.
通过模拟生理条件体外实验,将β-淀粉样蛋白1-42(Aβ42)与EGCG和4种茶黄素单体分别按照1︰1和1︰5比例进行孵育,采用硫黄素T荧光检测β-折叠结构的生成量,结果表明,EGCG与茶黄素均能明显降低β-折叠结构生成,从而抑制Aβ42聚集;此外,通过建立20μmol·L~(-1) Aβ42诱导人神经母细胞瘤SH-SY5Y细胞损伤模型,将不同浓度的EGCG或茶黄素(10、50、100μmol·L~(-1))处理后,MTT法检测细胞存活率,同时,检测细胞内活性氧(ROS)、谷胱甘肽过氧化物酶(GSH-Px)及丙二醛(MDA)等抗氧化指标,结果表明,EGCG与茶黄素可抑制因Aβ42诱导SH-SY5Y细胞活力下降及氧化损伤。体内实验中,通过10mg·kg~(-1)·d~(-1)的EGCG或茶黄素处理快速老化模型小鼠(SAMP8),10周后检测小鼠血清Aβ42及晚期糖基化终末产物(AGEs)含量,结果表明,EGCG和茶黄素可显著降低SAMP8小鼠血清中Aβ42及AGEs含量。本研究表明了茶黄素和EGCG可抑制Aβ42聚集纤维化及Aβ42导致的神经氧化损伤,从而对阿尔茨海默病具有一定保护作用。 相似文献
11.
12.
Mitochondrial dysfunction contributes to the pathogenesis of kidney injury related with cardiovascular disease. Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) protects renal tubular cells by upregulating nuclear factor erythroid 2-related factor 2 (Nrf2). AMP-activated protein kinase (pAMPK)-mediated phosphorylation and sirtuin 1/3 (SIRT1/3)-mediated deacetylation are required for PGC-1α activation. In the present study, we aimed to investigate whether omega-3 fatty acids (FAs) regulate the expression of mediators of mitochondrial biogenesis in 5/6 nephrectomy (Nx) rats. Male Sprague-Dawley rats were assigned to the following groups: sham control, Nx, and Nx treated with omega-3 FA. The expression of PGC-1α, phosphorylated PGC-1α (pPGC-1α), acetylated PGC-1α, and factors related to mitochondrial biogenesis was examined through Western blot analysis. Compared to the control group, the expression of PGC-1α, pAMPK, SIRT1/3, Nrf1, mTOR, and Nrf2 was significantly downregulated, and that of Keap 1, acetylated PGC-1α, and FoxO1/3, was significantly upregulated in the Nx group. These changes in protein expression were rescued in the omega-3 FA group. However, the expression of pPGC-1α was similar among the three groups. Omega-3 FAs may involve mitochondrial biogenesis by upregulating Nrf1 and Nrf2. This protective mechanism might be attributed to the increased expression and deacetylation of PGC-1α, which was triggered by SIRT1/3. 相似文献
13.
107份小麦历史品种抗穗发芽基因型的检测 总被引：1，自引：0，他引：1
为了在历史小麦品种中鉴定出抗穗发芽的种质材料,利用与小麦抗穗发芽相关的分子标记Vp1A3和Vp1B3对107份我国小麦历史品种的穗发芽抗性进行综合筛选。结果表明,属于抗穗发芽单倍型Vp-1Agm的材料有3份,分别是郑州742、陕农17、内乡5号;属于抗穗发芽单倍型Vp-1Bb的材料有10份,分别是跃进5号、泰山1号、济南12、泾阳60、复壮30、丰抗15、济农2号、北京8号、济南矮6号、京130;属于抗穗发芽单倍型Vp-1Bc的材料有48份。在具有发芽指数的77份材料中,平均发芽指数小于10%的抗穗发芽基因型材料有5份,分别为安选2号、烟农15、郑州683、郑州742、克群。该研究结果为小麦抗穗发芽育种中抗性亲本的选择提供了基因水平的依据,拓宽了小麦抗穗发芽育种亲本的选择范围。 相似文献
14.
González-Espinosa de los Monteros LA Ramón-Gallegos E Torres-Torres N Mora-Escobedo R 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(4):355-362
Germination of soybeans increases the bioavailability of some nutrients. An evaluation was done to determine if germination
increased the anti-adipogenic and lipolytic effects of soybean. Soybeans were germinated for 0 to 6 days and protein concentrates
extracted from beans germinated at each period. Soy protein concentrates can retain notable amounts of phytochemicals with
anti-adipogenic activity. For this reason, it was evaluated the effect of protein hydrolysates with and without phytochemicals
in the adipocyte-like cells after 3T3-L1 (murine fibroblasts) cell line differentiation. Cell viability decreased with exposure
to the germinated soybean protein hydrolysates during the differentiation stage, but not during the fibroblast or mature adipocyte
stages. Adipogenesis and triglycerides accumulation were strongly inhibited by the hydrolysate from soybeans germinated for
2 days (with ethanol-soluble phytochemicals), when compared to ungerminated soybean. Adipolysis increased with exposure to
hydrolysates from beans germinated for 2 days (with phytochemicals) and 5 days (without phytochemicals). Germinated soy protein
hydrolysates had an effect on inhibition of lipid storage in adypocites and increasing lipolysis, which was improved by changes
of the protein and increased phytochemical content due to germination. 相似文献
15.
Jiugang Yuan Yuanyuan Yu Qiang Wang Xuerong Fan Shaoyu Chen Ping Wang 《Fibers and Polymers》2013,14(8):1254-1260
For a long time, alkali is the main modification reagent for ramie modification due to its good effect and low cost. However, the large consumption of alkali in the processing leads to a serious pollution to the environment. To develop a new eco-friendly modification method, a mixed green solvent composed of 95 wt% 1-butyl-3-methylimidazolium chloride ionic liquid and 5 wt% water was employed in the paper. The swelling ratio, surface composition and crystal index was studied in detail with video microscope, FTIR and XRD analysis. Results showed that the solvent system had a distinct swelling effect on ramie. The crystal index of ramie fiber decreased from 74.2 % to 54.5 % after the treatment. Otherwise, the modification also removed some gummy substances including 75 % content of pectin in ramie. These changes improved the wetability and dyeing properties of ramie. However, the treatment also did much harm to the tensile strength. 相似文献
16.
An ionic liquid (IL)-water mixture employed to treat lignocellulosic biomass is promising. The addition of water decreases viscosity and process cost so as to improve the IL practical application. In this work, effects of temperature (50-170 °C), water content (0-80 wt%), treating duration (0.5-4 h) and pressure (0.1-3.2 MPa) on treating legume straw process using a 1-butyl-3-methylimidazolium chloride ([C4mim]Cl)-water mixture were experimentally investigated. Legume straw was found to be partially dissolved, and the dissolved substances can be flocculated by adding the coagulating agent—water (equal to volume of the solution). For this process at 0.1 MPa, the maximum 29.1 wt% legume straw is dissolved in the [C4mim]Cl-water mixture with water content of 20 wt% at 150 °C during 2 h, which is much higher than 9.8 wt% using pure [C4mim]Cl. A hemicellulose-free lignin-rich material (64.0 wt% lignin and 35.3 wt% cellulose) is obtained by adding the water. Even for 0.5 h, 22.3 wt% of legume straw is dissolved in the case of water content of 20 wt%, 150 °C and 0.1 MPa. High pressure favors the dissolution of legume straw but lignin content in the residue has no obvious change. The addition of proper amount of water facilitates the dissolution of legume straw and a relative rapid dissolving rate can be achieved in a [C4mim]Cl-water mixture. There are great differences in chemical and physical properties between legume straw and the obtained samples (residue and floc) due to the dissolution and reconstitution. 相似文献
17.
Wheat gluten films were cast from aqueous dispersions containing 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as cross-linking reagents and glycerol as a plasticizer. Cross-linking was carried out to improve film properties such as water sensitivity and tensile strength. Films were characterized by measuring protein and water content, amount of amino groups, swelling of the films in water, and mechanical properties such as tensile strength (σmax) and strain at maximum stress ( at σmax). The use of different ratios of EDC to COOH resulted in different tensile properties and different percentage of swelling, which was attributed to the degree of cross-linking in the film. At a ratio of EDC/NHS/COOH=0.5/0.5/1, films had a water content of 10–11.5% and showed the highest σmax (2.8±0.9 MPa), the lowest at σmax (142±67%), and the lowest swelling (46%) compared to σmax=1.7±0.4 MPa, at σmax=257±63%, and swelling=68% for native gluten films. 相似文献
18.
《Journal of Cereal Science》1995,21(2):137-144
Two recombinants between Gli-A1 and Glu-A3 prolamin genes and seven between Gli-B1 and Glu-B3 obtained from two crosses of durum wheat were classified on the basis of the prolamin alleles at the Glu-A1, Gli-A1, Glu-A3, Gli-A2, Glu-B1, Gli-B1 and Glu-B3 loci. The separate effects on gluten strength of gliadin genes at Gli-1 and low Mr glutenin subunit genes at Glu-3 were estimated by comparing the SDS-sedimentation test values of the recombinants with those of the same progeny having the same prolamin composition, except for the recombined alleles. The results showed clearly that low Mr glutenin subunit genes at Glu-A3 and Glu-B3, rather than gliadin genes at Gli-A1 and Gli-B1, were responsible for the differences in gluten strength. 相似文献