共查询到20条相似文献，搜索用时 15 毫秒
1.
G. H. Hudson A. V. Omprakash K. Premavalli G. Dhinakar Raj 《British poultry science》2017,58(2):192-199
1. Guinea fowl hens were inseminated weekly once with two doses of spermatozoa (75 million and 100 million) in two different diluents, Beltsville poultry semen extender (BPSE), and Instruments for Veterinary Medicine (IMV), each with and without pre-insemination vaginal douching. Per cent fertility, hatchability, dead germ, dead in shells along with data on sperm egg interaction and vaginal microbial counts were recorded.
2. Artificial insemination had significantly improved the per cent fertility and hatchability compared to natural mating. Dose dependent improvement in fertility was noticed with both diluents.
3. There was a beneficial effect of vaginal douching, which was more pronounced at lower insemination doses.
4. For optimum fertility and hatchability in guinea fowl, insemination of 75 million spermatozoa diluted in BPSE once in 4 d and 100 million spermatozoa diluted in BPSE or IMV once in 5 d coupled with vaginal douching is recommended.
2.
1. White Leghorn hens were artificially inseminated twice weekly with undiluted semen or semen diluted (1:3) in Tris buffer, pH 6.8 or saline solution (9 g NaCl/l); with or without pre-insemination douching of the vagina with a solution of ampicillin. 2. Pre-insemination douching reduced the bacterial load of the vagina, resulting in an improvement in fertility and hatchability, with a proportionate reduction in the embryonic mortality. 3. The fertilising and hatching abilities of saline-diluted semen were comparable with those of undiluted semen. 4. Dilution of semen with Tris buffer significantly reduced fertility, but not hatchability. 相似文献
3.
Paulenz H Söderquist L Adnøy T Nordstoga AB Andersen Berg K 《The Veterinary record》2005,156(12):372-375
The effect of vaginal and cervical deposition of frozen-thawed semen on the fertility of sheep was tested in a field trial in which 543 Norwegian crossbred ewes aged between six months and five-and-a-half years from 10 farms were inseminated after natural oestrus. Cervical insemination with 200 x 10(6) spermatozoa resulted in 25-day non-return and lambing rates of 75.4 and 72.7 per cent, respectively, and vaginal insemination gave rates of 71.3 and 67.4 per cent; the cervical inseminations produced significantly higher lambing rates (P=0.04). There were significant differences between the lambing rates for different rams (P=0.006) and different farmers (P=0.003), and there was a significant interaction between farmer and deposition site (P=0.03). After vaginal insemination fertility was encouragingly high, but the results varied with the farmer, and different flock and management conditions. 相似文献
4.
The purpose of this study was to determine whether fertilisation of Bobwhite quail could be secured by artificial insemination. After this proved to be feasible, the period between insemination and the onset of laying fertile eggs, the time during which fertile eggs were laid and levels of fertility and hatchability of eggs obtained from hens inseminated at weekly intervals over an extended period of time were determined.
Semen was collected from Bobwhite quail males by simultaneously applying pressure to both the left and right sides of the vent without prior stimulation. Upon collection, the semen was diluted with 0.9 per cent saline solution to prevent dehydration. Semen volume measurements were made with a tuberculin syringe to which was attached a piece of graduated, fine‐bore plastic tubing. Females were artificially inseminated by placing the diluted semen in the posterior opening of the everted oviduct.
Mean duration of fertility of the eggs from six trials, two of which utilised only a single insemination, one of which was the final insemination of a series of weekly inseminations and three of which utilised two inseminations within a period of 24 hr, was 9.3 days. Percentage fertility of eggs improved considerably after the first trial probably due to improvement of technique and gain in experience. Average fertility of eggs for the six trials was 64–9 per cent.
The average time interval between insemination and the appearance of the first fertile egg was 3.4 days. Peak fertility was reached by the fourth day following insemination.
Fertility of eggs averaged 62.2 ±3.5 per cent and their hatchability averaged 77.2 ±1.4 per cent over a period of 31 weekly inseminations. These values were comparable to 60.2 per cent fertility and 78.9 per cent hatchability which were obtained from natural matings. 相似文献
5.
Two experiments were conducted to determine the effects of delaying sexual maturity of broiler breeder males on subsequent reproductive performance. Four dietary protein levels (16.0, 12.4, 8.9 and 5.1 per cent) were fed during the growing period. Thereafter, all males were fed a diet containing 17.0 per cent protein.
Sexual maturity was delayed 4 and 8 weeks, respectively, by feeding the 8.9 and 5.1 per cent protein diets, but was not affected by feeding the 12.4 per cent protein diet. Viability was superior for males receiving the diets containing 8.9 or 5.1 per cent protein. The low protein diets had no adverse effects on semen production, fertility or hatchability following recovery from the protein restriction. 相似文献
6.
The effect of the deposition site and the numbers of sperm on the fertility of sheep was tested in a field trial in which 1292 Norwegian crossbred ewes aged between six months and five-and-a-half years from 52 farms were inseminated with liquid semen after natural oestrus. Cervical insemination with 150 x 10(6) and 75 x 10(6) spermatozoa resulted in 25-day non-return rates of 63.7 and 56.1 per cent, and vaginal insemination gave non-return rates of 63.3 and 56.6 per cent, respectively. There was no significant difference between the cervical and vaginal inseminations, but the inseminations with 150 x 10(6) spermatozoa gave significantly higher non-return rates (P=0.004). There were significant differences between the non-return rates for different rams (P<0.0001) and farmers (P=0.0002) but the age of the ewe had no significant effect. 相似文献
7.
1. From 1992 to 2003, selected (S) and control lines (C) of the laying Brown Tsaiya duck (Anas platyrhynchos) were simultaneously maintained under the same standardised conditions of feeding and management. 2. The selection objective was to increase the number of fertile eggs after a single artificial insemination (AI) with pooled Muscovy semen. From generations G1 to G11, 2452 and 2022 female ducks, in S and C lines, respectively, were measured and recorded. In the S line, the percentage selected varied between 20.2 and 34.3% in females and between 7.2 and 20.8% in males. 3. Selection for number of fertile eggs had a correlated effect of increasing the parameter tau of the logistic curves which fitted the daily variations (d 2 to 15) in fertility or hatchability on the basis of eggs set. The differences S-C for the estimates of the times of half maximal fertility and hatchability increased by 0.41 and 0.37 d per generation between G1 and G11, respectively. 4. The highest increases of fertility per day rates after a single AI were observed between d 5 and 11. Moreover, in the selected line, fertility rate was higher than, or equal to, 90% in d 2 from G8. The same tendencies were observed for the changes in the evolution of hatchability on the basis of eggs set. 5. Selection increased fertility and hatchability according to the egg set rates, especially for d 2 to 8 after AI. Hatchability of fertile eggs was not impaired, confirming that selection for one AI per week was possible in this strain of laying ducks. 相似文献
8.
Evaluation of glucose as a cryoprotectant for boar semen 总被引：2，自引：0，他引：2
Fertility parameters of boar spermatozoa were evaluated in vitro, after freeze-thawing the semen in three different extenders containing permeable and non-permeable cryoprotectants: A (111.0 mM Tris, 31.4 mM citric acid, 185.0 mM glucose, 20 per cent egg yolk, 3 per cent glycerol and 100 iu/ml penicillin G); B (200 mM Tris; 70.8 mM citric acid, 55.5 mM glucose, 20 per cent egg yolk, three per cent glycerol and 100 iu/ml penicillin G); C (200 mM Tris, 70.8 mM citric acid, 55.5 mM fructose, 20 per cent egg yolk, 3 per cent glycerol and 100 iu/ml penicillin G). The freeze-thawing techniques were the same for each extender. Eight ejaculates from four boars were obtained; the sperm-rich fraction of each ejaculate was extended in each of the three media at a final concentration of 400 x 106 sperm/ml, loaded into 0.5 ml straws and frozen at a rate of 30 degrees C/minute to -196 degrees C. The straws were thawed at 60 degrees C for eight seconds. Sperm motility, acrosomal integrity and in vitro sperm penetration through the zona pellucida of gilt oocytes matured in vitro were evaluated. The motility of unfrozen spermatozoa was 93.1 per cent compared with 60.7 per cent, 48.2 per cent and 35 per cent for sperm frozen in extenders A, B and C respectively; these values were all significantly different (P<0.05). There was no significant decline in sperm motility after incubation for 30 minutes in extender A, but there were significant decreases in sperm motility after 30 minutes of incubation in B and C. The percentage acrosomal integrities were 97.2 per cent for the control and 45.5 per cent, 30.3 per cent and 16.8 per cent for the frozen-thawed spermatozoa in extenders A, B and C respectively. The results of the in vitro penetration assay were 80.7 per cent when using control spermatozoa, and 42.2 per cent, 18.4 per cent and 3.3 per cent when using frozen-thawed spermatozoa in extenders A, B and C respectively 相似文献
9.
The fertility of 646 ewes and gimmers bred by laparoscopic artificial insemination (LAI) in the autumn of 2006 was investigated using a questionnaire and individual ewe breeding records kept for 13 commercial sheep flocks that used LAI routinely. Overall, the pregnancy rate was 66 per cent, but it was highest in ewes bred for the fourth time. Technical aspects of LAI influenced fertility: pregnancy rates were 70 per cent for ewes bred using frozen semen compared with 58 per cent when fresh semen was used (P≤0.01), and 74 per cent of ewes that travelled to an artificial insemination centre for mating conceived, compared with 62 per cent that remained on their own farm (P<0.01). Higher doses of equine chorionic gonadotrophin (>400 iu) used for oestrus synchronisation reduced pregnancy rates to only 49 per cent (P<0.001). However, the largest effect was associated with shepherds gathering, handling and treating breeding ewes four to six weeks before mating; pregnancy rates were 54 per cent among ewes where this was carried out, compared with 74 per cent for ewes not treated in this way (P<0.01). 相似文献
10.
The objective of this study was to investigate whether butylated hydroxytoluene (BHT) could be used as a suitable supporter or alternative of egg yolk during preservation of goat spermatozoa. Three in vitro experiments and a fertility test were conducted to evaluate the effect of BHT on viability of chilled‐stored semen as well as motility and kidding rate of frozen‐thawed spermatozoa. In the first two experiments, ejaculates (n = 30/experiment) were collected from 10 bucks, split, diluted with egg yolk‐based and egg yolk‐free extenders supplemented with or without 0.3, 0.6, 2, 5 and 8 mm BHT and stored at 5°C for 168 h. In the third experiment, 30 ejaculates were collected from the above‐mentioned bucks, split and diluted with egg yolk‐free extenders supplemented with or without 0.3, 0.6 and 0.9 mm BHT and egg yolk‐based extenders supplemented with or without 5 mm BHT. Diluted semen was cooled to 5°C over a period of 4 h, frozen and thawed in the form of 0.3‐ml pellets. In the fertility test, 75 ejaculates were collected from two proven fertile bucks, split, diluted with egg yolk‐free extenders containing 0.6 mm BHT and egg yolk‐based extenders supplemented with or without 5 mm BHT, frozen and thawed as described above. An insemination volume of 0.6 ml containing 120–140 × 106 progressively motile spermatozoa was used for a single cervical insemination of cloprostenol‐synchronized does (n = 230). The results showed that addition of 5 mm BHT to egg yolk‐deficient (2.5%) extenders significantly improved viability of chilled‐stored semen together with motility (48.5%) and fertility (62.5%) of frozen‐thawed spermatozoa. Replacement of egg yolk in semen extenders by 0.6 mm BHT could sustain not only viability of chilled‐stored semen but also post‐thaw motility (47.5%) and fertility (53.75%) of frozen‐thawed spermatozoa. In conclusion, supplementation of semen diluents with BHT can ameliorate preservability of goat sperm. 相似文献
11.
J C Segura J S Gavora J L Spencer R W Fairfull R S Gowe R B Buckland 《British poultry science》1988,29(3):545-553
1. Males from strains selected for high egg production (and other economic traits) and from unselected control strains were used to determine the frequency of shedding of lymphoid leukosis virus (LLV) into semen. The effect of the male's LLV status on semen production, fertility and hatchability was also examined in males of the unselected control strains. 2. The frequency of detection of exogenous LLV in semen by the phenotypic mixing test, and high concentrations of the viral group specific antigen in feather pulp by the complement fixation test, were both higher in control strains than in strains selected for high egg production. 3. Semen production was not reduced in LLV-shedding males. 4. Significant associations of LLV shedding with higher incidence of abnormal spermatozoa and reduced fertility were found in some populations but not in others. No significant effect of LLV shedding on hatchability was detected. 5. Tests for group specific antigen in feather pulp proved useful in identifying males that shed LLV in semen. 相似文献
12.
Fertility of a stallion with low sperm motility and a high incidence of an unusual sperm tail defect 总被引：1，自引：0，他引：1
At the beginning of the breeding season an eight-year-old standardbred stallion had semen with virtually zero sperm motility and an approximately 90 per cent incidence of midpiece and tail defects. The motility of the sperm improved to 7 per cent when semen was collected daily but its morphology did not improve. Electron microscopy revealed that the defects consisted mainly of a loss of microtubules in the axoneme and of disorganised midpieces. A pregnancy rate of 24 per cent per cycle and 44 per cent for the season was achieved in 32 mares after the insemination of whole ejaculates collected from the stallion frequently. The fertility was much higher than would have been expected from the characteristics of the semen. It is concluded that this sperm defect, reminiscent of the 'Dag defect' in bulls and the defect in T-locus mice, does not render the animal infertile. 相似文献
13.
不同稀释液对藏猪精液℃保存效果的影响 《畜牧与饲料科学》2016,37(10):22-22
为探讨不同精液稀释液对4℃保存藏猪精液的效果,保证优良藏猪种公猪精液的合理、高效利用,该试验选用5种常用的猪精液常温保存稀释液,并在藏猪精液4℃保存至第5天时检查藏猪精子活力、精子畸形率、精子顶体完整率、精子质膜完整率等。结果表明,在精液保存过程中,2号液对藏猪精液的保存效果最佳,精子活力在保存至第5天时仍然保持在0.54,且藏猪精子活力、精子畸形率、精子顶体完整率、精子质膜完整率均高于其余4组（P〈0.05）。该试验研究初步筛选了4℃保存藏猪精液的稀释液,为今后藏猪精液的生产应用奠定了基础。 相似文献
14.
The extenders and freezing rates from three different freezing protocols were combined and compared to each other in order to study the post-thawing acrosome integrity and fertility of frozen dog sperm. A commercial bovine TRIS-base extender (TRILADYL) and two self-made canine semen extenders (Norwegian and Dutch) were combined with a conventional bovine and two canine freezing regimes, and acrosome integrity of frozen/thawed spermatozoa was assessed by fluorescein isothiocyanate conjugated peanut agglutinin staining (FITC-PNA). Differences between freezing/thawing protocols were reflected in the proportion of cells with acrosomal damage and not based on motility results. It was concluded that during dog semen cryopreservation extenders had less influence on the post-thawing sperm quality than did the freezing rates. The optimal extender/freezing rate combination (TRILADYL/Norwegian) was used in the clinical practice to evaluate the fertility of frozen sperm administered by intrauterine insemination using a surgical approach. The pregnancy rate was 57% (4/7), but the average litter size was low (2.8). This may have been due to the insufficient sperm numbers contained in an insemination dose and/or to the incorrect timing of artificial insemination (AI). The final conclusion is that the commercial bovine extender is useful for freezing dog semen, and the TRILADYL/Norwegian freezing protocol is recommended as the most advantageous combination for the freezing of canine semen in the clinical practice. 相似文献
15.
Akhter S Ansari MS Andrabi SM Rakha BA Ullah N Khalid M 《Reproduction in domestic animals》2012,47(5):815-819
Egg yolk is routinely used as a cryoprotectant in semen extenders. However, it may contain cryoprotective antagonists, and there are hygienic risks associated with its use. Proteins of plant origin, like soya-lecithin, lack these hazards. The aim of this study was to use soya-lecithin as a cryoprotectant in extender and to investigate its effects on in vitro quality and in vivo fertility of buffalo semen. Semen from three buffalo bulls was frozen in tris-citric extender containing 5.0%, 10% or 15% soya-lecithin or 20% egg yolk. Sperm motility, plasma membrane integrity and viability were assessed post-dilution, pre-freezing and post-thaw. In Post-dilution and pre-freezing, the values for motility, plasma membrane integrity and viability remained higher (p ≤ 0.05) in extenders containing 10% soya-lecithin and control compared with extender containing 5% and 15% soya-lecithin. However, motility, plasma membrane integrity and viability were higher (p < 0.05) in extender containing 10% soya-lecithin compared with control and extenders containing 5% and 15% soya-lecithin. Semen from two buffalo bulls was frozen in tris-citric extender containing either 10% soya-lecithin or 20% egg yolk. Higher (p < 0.05) fertility rate was recorded in buffaloes inseminated with semen containing 10% soya-lecithin (56%) compared with 20% egg yolk (41.5%). The results suggest that 10% soya-lecithin in extender improves the freezability and fertility of buffalo bull spermatozoa and can be used as an alternate to egg yolk in cryopreservation of buffalo semen. 相似文献
16.
Bettina T. Olivieri Brenda C. Love Grant B. Rezabek Catherine G. Lamm Dickson D. Varner Mark E. Payton G. Reed Holyoak 《Journal of Equine Veterinary Science》2011,31(11):655-660
Taylorella equigenitalis is a gram-negative coccobacillus and the causative agent of a transmissible venereal disease in horses known as contagious equine metritis. Outbreaks of contagious equine metritis have been documented in various countries since 1977, with the most recent discovery in the United States in December 2008. During disease occurrences, culturing semen samples for T equigenitalis before breeding may help to prevent transmission of this disease; however, little is known about the antimicrobial activity of equine semen extenders against the organism. The purpose of this study was to investigate the infectivity levels of T equigenitalis in three equine semen extenders inoculated with known concentrations of the organism. The semen extenders used for this study included INRA 96, E-Z Mixin BF, and VMDZ. In addition, Timentin was added to INRA 96 at three different concentrations (0.5, 1.0, and 1.5 mg/mL) to investigate possible synergistic effects of antibiotic supplementation of extenders. Results were based on the visual counting of the colonies on chocolate Eugon agar plates. Both INRA 96 (with added Timentin) and VMDZ (as supplied by the manufacturer) significantly reduced the numbers of T equigenitalis isolated from semen extenders as compared with INRA 96 (as supplied by the manufacturer) or the antibiotic free E-Z Mixin BF. Our findings indicate that INRA 96 (with added Timentin) or VMDZ may significantly decrease the growth of T equigenitalis in extended semen; however, it is also important to consider the possible effects of antibiotic supplemented extenders on sperm longevity and fertility in addition to eliminating specific pathogens in semen. 相似文献
17.
Canisso IF Carvalho GR Morel MD Ker PG Rodrigues AL Silva EC Coutinho Da Silva MA 《Equine veterinary journal》2011,43(2):179-183
Reasons for performing study: As mule production is often concentrated in remote areas of the world, a simplified semen cryopreservation protocol is required. Aim: To compare the seminal parameters of cryopreserved donkey semen in lactose‐EDTA and lactose‐yolk extenders and the fertility rates on horse mares. Methods: Trial 1: Sperm total and progressive motility, vigour (scale 0–5), morphology (major and minor defects) and plasma membrane integrity (HOST) were evaluated in 25 ejaculates from 5 donkey jacks immediately after collection (raw), after chilling to 5°C (chilled) and after freezing/thawing. The semen was mixed with skimmed‐milk extender, centrifuged, and then re‐suspended in lactose‐EDTA or lactose‐yolk extender. Semen was loaded into 0.5 ml straws and chilled to 5°C for 1 h, after which samples were either evaluated (chilled semen) or placed above liquid nitrogen for 20 min prior to immersion. Seminal parameters were evaluated by ANOVA and Tukey's test. Trial 2: Cryopreserved semen from 3 males was used to inseminate 53 mares at 60 oestrous cycles randomly assigned to lactose‐yolk (n = 30 cycles) or lactose‐EDTA (n = 30 cycles) extenders. Pregnancy diagnosis was performed 15 and 25 days post ovulation. The pregnancy rates were compared using Chi‐squared tests. Results: Trial 1: No significant differences were evident in any seminal parameters between extenders after either chilling or cryopreservation. Total and progressive motility were significantly (P<0.05) lower in cryopreserved semen than raw and chilled semen for both extenders. Trial 2: Pregnancy rates did not significantly differ between extenders (lactose‐EDTA extender 53.33 and 43.33%; lactose‐yolk 50.0 and 46.66% for Days 15 and 25 post ovulation, respectively). Conclusions: Cryopreservation of donkey semen using the simplified lactose‐yolk extender resulted in similar seminal parameters and fertility rates when compared to lactose‐EDTA extender. Potential relevance: Lactose‐yolk extender may be advocated as a simple, easy to prepare extender, for use in geographically isolated enterprises producing mules throughout the world. 相似文献
18.
Fukui Y Kohno H Togari T Hiwasa M Okabe K 《The Journal of reproduction and development》2008,54(4):286-289
The present study aimed to compare the fertility of ewes intrauterinally inseminated with frozen-thawed semen using a soybean-based semen extender (AndroMed) with those of ewes intrauterinally inseminated with frozen-thawed semen using a Tris-based extender containing either egg yolk or BSA. Suffolk ewes (n=104) were treated with an intravaginal sponge containing 40 mg fluoroprogesterone acetate (FGA) for 12 days and an intramuscular injection of 500 IU equine chorionic gonadotropin to induce estrus and ovulation during the non-breeding season (July, 2007). Intrauterine insemination was carried out 40-46 h after removal of the FGA sponge (n=90), regardless of the incidence of estrus. The pregnancy rates were not significantly different among the semen extenders containing egg yolk (64.5%) or BSA (58.6%) and AndroMed extender (56.7%). The lambing rates (64.5, 55.2 and 56.7% for the semen extenders containing egg yolk, BSA and AndroMed, respectively) and prolificacy (1.59 to 1.75) were also not significantly different. The present results indicate that an egg yolk-containing semen extender can be replaced with the non-animal derived extender AndroMed, which could be used for intrauterine insemination using frozen-thawed ram semen without reducing fertility. 相似文献
19.
Lukaszewicz E 《British poultry science》2001,42(3):308-314
1. The objective of the present study was to determine if the age of semen donors affects the susceptibility of spermatozoa to freezing and whether DMF (dimethyl formamide) inseminated with freeze-thawed gander semen decreases fertility. 2. Semen was collected 3 times a week by dorsal-abdominal massage from two groups of White Italian ganders: 3 and 2 years-old. Both samples were diluted, mixed with DMF to a final concentration of 6% (v/v), pre-frozen and transferred into LN2. 3. Twice a week, the freeze-thawed semen was used for insemination of two groups of geese at a dose of 4 to 16 million live morphologically normal spermatozoa. One group was inseminated immediately after thawing, the 2nd with semen from which the DMF was removed. 4. Donor age had no effect on the spermatozoa's aptitude for freezing. The differences in quality and quantity of fresh and freeze-thawed semen produced by 3 or 2 year-old ganders were not significant. 5. The presence of DMF in the inseminated freeze-thawed semen did not affect the reproductive efficiency of spermatozoa. The fertility rate obtained with semen inseminated either with or without the cryoprotectant averaged 92.9% and 87.2% respectively. The hatchability of set eggs was 81.1% and 79.9% and, the hatchability of fertile eggs amounted to 87.3% and 89.4%. 相似文献
20.
F Van Wambeke 《British poultry science》1972,13(2):179-183
Semen obtained from Rhode Island Red males was stored for 3 and 24 h in fresh and freeze‐dried diluent. Fertility and hatchability of eggs laid from the 2nd to 8th d inclusive after a single insemination was tested with hens of proven fertility. The fertilising power of diluted semen after storage did not differ significantly from the undiluted control, but the hatchability of the fertile eggs was significantly reduced after 24 h storage in the freeze‐dried diluent. 相似文献