共查询到20条相似文献，搜索用时 78 毫秒
1.
杨朴丽摘录 《柑桔与亚热带果树信息》2014,(3):73-73
据《园艺学报》2014年第2期《“黄冠”梨采后热处理和钙处理对其钙形态及细胞壁物质代谢的影响》（作者王伶俐等）报道,以“黄冠”梨为材料,研究了采后热处理（38℃热空气24小时）,钙处理（6％CaCl2浸果15分钟）及热＋钙处理（6％CaCl2＋38℃）对果实低温贮藏过程中钙形态及细胞壁物质代谢的影响,以清水浸果为对照。 相似文献
2.
高温胁迫下糙皮侧耳胞内钙离子对热激蛋白基因表达的调控 总被引：1，自引：0，他引：1
以糙皮侧耳(Pleurotus ostreatus)菌株CCMSSC00389为材料,研究高温胁迫(40℃、120min)下钙离子的响应及其对热激蛋白的调控。在高温胁迫条件下,在培养基中添加胞内钙离子螯合剂、胞外钙离子螯合剂、钙调蛋白抑制剂、质膜钙通道抑制剂、三磷酸肌醇受体拮抗剂,利用钙离子特异性荧光探针检测菌丝体胞内钙离子含量,采用荧光定量PCR检测热激蛋白的基因表达量。结果表明,高温胁迫促进胞内钙离子浓度升高,增加Hsp60、Grp78、Hsp90、Hsp104等4种热激蛋白的基因表达量;培养基中添加钙离子螯合剂或抑制剂会降低胞内钙离子浓度,抑制这些热激蛋白的基因表达。研究结果表明高温胁迫会诱导糙皮侧耳胞内钙离子浓度升高,参与信号转导,从而调控热激蛋白的表达来缓解高温胁迫引起的伤害。 相似文献
3.
钙是苹果必需的营养元素,参与细胞壁的组成,非原生质体中的钙在形成细胞壁的多糖与蛋白质复合体中起粘和作用,从而增强了膜的稳定性。同时,由于钙固着于原生质表面和细胞壁的交换点上,使其渗透性减少。因而果实组织中Ca^2＋与成熟衰老密切相关,同样Ca^2＋可以延迟叶片的早衰。一般果实组织中Ca^2＋含量高时,可延迟果实衰老,提高硬度,增强耐贮性。 相似文献
4.
5.
温度逆境胁迫可以诱导热激蛋白的表达,而热激处理能够减轻果实冷害。为了探讨温度逆境对香蕉热激蛋白基因表达的调控、辨析香蕉热激蛋白基因的表达与果实冷害的关系,采用同源序列法分离了香蕉果皮HSP70基因序列-根据已经报道的HSP70基因的氨基酸保守序列设计引物、以香蕉果皮总RNA为模板,用RT-PCR方法克隆香蕉的HSP70 cDNA,Northern杂交分析该基因在不同贮藏温度下的表达特征。结果得到2个序列不同的HSP70基因片段,分别命名为Ma-HSP70-1和Ma-HSP70-2,Ma-HSP70-1和Ma-HSP70-2之间的碱基同源性为86.9%,氨基酸同源性为97.1%。Northern杂交的结果表明,38℃短期热激处理诱导了Ma-HSP70-2基因的表达,低温冷藏能使2个基因的表达增强。并初步认为Ma-HSP70可能与香蕉果实耐冷性有关。 相似文献
6.
本书是针对目前我国果实品质下降和生理病害日趋严重的现实编写的。全书共分六章，比较详细地总结了果树缺钙症、果实钙素营养水平的调节，Ca^2＋在树体内的运转与分配规律，钙与花芽分化、花粉萌发和花粉管生长、结实及发育之间的关系，钙参与果实成熟衰老和抗逆性的调控机制，以及典型缺钙症——苹果苦痘病研究的评述等。 相似文献
7.
8.
9.
低温胁迫下不同耐冷性番茄品种幼叶细胞Ca^2＋分布变化的差异 总被引：8，自引：2，他引：6
采用焦锑酸钙沉淀的电镜细胞化学方法，研究了低温胁迫与冷锻炼下不同耐冷性番茄品种幼叶细胞Ｃａ＾２＋定位分布的变化。结果表明：正常生长条件下，番茄幼叶细胞Ｃａ＾２＋主要存在于液泡和细胞间隙内，细胞质中含量很低，且耐冷性不同品种之间无明显差异。经５℃低温处理２４ｈ后，耐冷品种细胞内Ｃａ＾２＋分布变化明显，胞内钙库（即液泡）释放Ｃａ＾２＋进入细胞基质，细胞间隙中仍有大量Ｃａ＾２＋存在；而冷敏感品种无明显变化。当处理４８ｈ后，耐冷品种细胞Ｃａ＾２＋分布趋向于恢复到处理前的状态，而冷敏感品种则在细胞内形成较大的钙沉淀颗粒，多分布于绿体被膜与质膜内侧，细胞间隙内Ｃａ＾２＋颗粒也集中聚集成团。昼１５℃／夜８℃条件下的冷锻炼对不同耐冷性番茄幼叶细胞内Ｃａ＾２＋分布的影响也表现不同。 相似文献
10.
意大利巴里大学的研究人员研究了转色期前后钙处理对鲜食葡萄贮藏期腐烂率和果实品质的影响。以鲜食葡萄品种Italia为材料，连续两年在着果至转色期、转色期至采收期对葡萄喷洒44％螯合态氯化钙，以不作处理为对照。结果表明，转色前钙处理果皮中Ca^2＋含量最高。 相似文献
11.
The effects of combinations of salinity (no salt, 2000 p.p.m. or 4000 p.p.m. of CaCl2 and NaCl, 1:1) and water table (30 cm, 60 cm or 90 cm from the soil surface), on the vegetative growth and tolerance of ‘Golden Japanese’ plum and ‘Mit Ghamre’ and ‘Balady’ peaches were studied. The plants were grown in lysimeters. The growth of the trunk, total shoot length, the increase in shoot length per cm and the fresh weight of top, root and total plant were reduced with increasing salinity of the irrigation water. The effect was accentuated when the plants were maintained at high water table level. The salinity treatments resulted in the death of 43%, 73% and 76% of the plants in the plum, and the ‘Mit Ghamre’ and ‘Balady’ peaches, respectively, indicating that the plum is more tolerant to salinity than the peach. The plants of the salinity treatments showed various symptoms of salt injury, such as leaf burn, defoliation, shoot die-back and finally death. In the peaches, salt injury started to occur in the first growing-season, whereas the salt injury appeared in the plum in the second growing-season. The symptoms were more pronounced in the 4000 p.p.m. treatment than in the 2000 p.p.m. treatment and were more pronounced at the high water table level. The salinity level was the predominant factor and the effect of the water table on the vegetative growth diminished with increase in the salinity level of the irrigation water. 相似文献
12.
13.
AIM：To establish a fast, accurate and economical technique for culturing mouse pulmonary arteriolar smooth muscle cells (PASMCs), and to explore the effects of hypoxia on the proliferation and apoptosis of the PASMCs. METHODS：In sterile condition, the pulmonary artery was isolated from the male BALB/c mice by digesting with collagenase I, and the cells were cultured in fetal bovine serum-coated flask. Centrifugal procedure was not used during the cell passage. The cell morphology was observed under an inverted phase-contrast microscope. α-Smooth muscle actin was identified by immunocytochemistry and immunofluorescence. The effects of hypoxia on the proliferation and apoptosis of the PASMCs were detected by CCK-8 assay and TUNEL assay. RESULTS：PASMCs were identified by the methods of immunocytochemistry, immunofluorescence staining and observation of morphology. Unlike the rat PASMCs with typical subcultured peak-vally pattern, the mouse PASMCs showed a lot different without a peak-vally pattern. The cells could be subcultured after 5 d to 7 d and there was 3 to 5 generations depending on the activity of the cells. CCK-8 assay demonstrated that the A values of PASMCs exposed to hypoxia increased after 24 h (P＜0.05) as compared with normoxia. TUNEL result showed that the apoptotic index of the PASMCs in hypoxia decreased after 24 h (P<0.05). CONCLUSION：This technique for obtaining cultured mouse PASMCs is simple, fast, accurate and economical. The digestion time is easy to control. Hypoxia promotes the proliferation and inhibits the apoptosis of PASMCs. 相似文献
14.
小檗碱和壳聚糖抗蔬菜病原真菌活性测定及复合膜制备 总被引：2，自引：0，他引：2
研究了小檗碱和壳聚糖对几种常见蔬菜病原真菌的抑制活性,以及以小檗碱和壳聚糖为主料制备复合膜的方法,并测定了该膜的药物释放效果。试验表明:低浓度(0.234 mg/mL)小檗碱即可抑制辣椒炭疽病菌(Vermicularia capsici)等5种蔬菜病原真菌的生长。20 mg/mL浓度壳聚糖对番茄灰霉病菌(Botrytis cinerea)的抑制率高达65%,而对其余4种果蔬病原真菌也有一定的抑制作用。为了集成这2种天然化合物的优点,制备了小檗碱-壳聚糖复合膜,该膜具有缓释功能,在模拟外部环境(磷酸缓冲液,pH 6.8)条件下,20 d小檗碱累计释放率接近25%,提示其在果蔬贮藏抗菌中的应用价值。 相似文献
15.
16.
Fresh onion seeds desiccated to 6.0% seed moisture content (SMC) were stored in various packaging materials under different storage conditions. Seeds packed in aluminum-laminated pouches beside those stored with silica gel at 25 °C maintained satisfactory germinability and vigour after 12 months. Desiccated seeds stored in moisture impervious containers produced more vigourous seedlings. Germination potential of onion seeds increased with reduced SMC besides storage in moisture impervious packets along with desiccants as physiological and biochemical attributes are regulated. Seed viability and vigour decreased with accelerated ageing due to increased lipid peroxidation, decreased activities of several free radical and peroxide scavenging enzymes. Electrical conductance of seed leachates also increased with ageing. Thus, adoption of appropriate storage temperature and moisture control techniques would significantly affect onion seed quality, which was due to minimum accumulation of free peroxide radicals and enhanced activity of free radical scavenging enzymes. 相似文献
17.
18.
19.
以大荔县冬枣种植区采集的发病枣果为试材,采用常规组织分离法进行病原菌的分离纯化,通过柯赫氏证病法则、形态学观察及分子生物学分析,研究鉴定了冬枣黑斑病和炭疽病的病原菌种类,并采用田间调查法对2种病害为害特征进行比较分析,以期为田间有效区分冬枣黑斑病和炭疽病为害症状,并快速判断其病原类型,及进一步深入开展精准防控提供参考和指导.结果表明:陕西大荔冬枣黑斑病的病原菌为细极链格孢(Alternaria tenuissima),炭疽病的病原菌为胶孢炭疽菌(Colletotrichum gloeosporioides).2种病害均主要为害枣果实,在枣果果腰或果肩上形成黑褐色病斑,可能同时存在且症状相近极易混淆,但在病斑表面、皮下及久置表型3个方面存在明显区别. 相似文献
20.
钾肥在小白菜和萝卜上的施用效果 总被引：6，自引：0，他引：6
采用盆栽试验方法研究了施用钾肥对小白菜、萝卜生长发育、产量、品质及钾素含量的影响。结果表明 ,施用钾肥对小白菜和水萝卜的株高、叶片数无显著的影响 ,但可提高水萝卜块根的产量 ,提高幅度为 31.0 4 %～ 97.93%。施用钾肥可提高小白菜和萝卜的蛋白质和VC含量 ,降低硝酸盐含量 ,增加植株的钾素含量 相似文献