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1.
卵黄提取液防治新城疫一传染性法氏囊病的试验研究 总被引：1，自引：0，他引：1
首次应用高免卵黄的三氯甲烷提取液在实验室保护试验成功的基础上对现地５８８，６８８只病鸡进行了治疗保护试验，对新城疫、传染性法氏囊病及两者混合感染鸡群的保护率为９６．４５％，试验结果表明，卵黄提取液的应用为防治ＮＤ－ＩＢＤ病鸡提供了一种可靠的手段。 相似文献
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目前,国内外均用平板凝集试验(AGGP)检测血清中抗体净化鸡白痢病。国内亦有用琼扩试验(AGP)检验鸡白痢病的报导,但用卵黄代替血清进行鸡白痢病净化检疫的研究未见报导。从《鸡白痢病诊断方法特异性及敏感性的研究Ⅱ血清和卵黄平板法琼扩法及卵黄平板法与琼扩法符合率比较试验》(《黑龙江畜牧兽医》1990 相似文献
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应用卵黄抗体稀释液先后对115群病鸡共68,315只作治疗试验,保护率为84.02%,其中效价在640以上的卵黄抗体稀释液,其保护率高达93.52%。使用卵黄抗体治疗新城疫病鸡是可行的. 相似文献
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我们在本市10余个鸡场的60000多只鸡群中,试用鸡传染性法氏囊病高免卵黄液对患鸡进行治疗,取得了满意效果,现报告如下。一、试验鸡:选自当地鸡场(户)患鸡传染性法氏囊病的30～37日龄京白823和星杂228鸡。二、传染性法氏囊病高免卵黄液:出山东省畜牧兽医总站提供。三、试验分组 相似文献
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1991年12月20日由吉林省科委组织省内有关专家对吉林省兽医科学研究所的鸡法氏囊病高免卵黄液的“鸡法氏囊病高免卵黄液和鸡法氏囊病一新城疫高免卵黄液”的制备与应用进行了鉴定。近年来鸡传染性法氏囊病(IBD)在我省乃至全国广泛暴发流行。给养鸡业造成严重威胁和损失。该课题组采用三株不同毒力的 相似文献
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应用PPA-ELISA检测了北京市某种鸡场三个父母代鸡群种卵卵黄传染性法氏囊病的抗体水平,结果表明。卵黄抗体效价与血清抗体效价基本相同,证实了运用卵黄替代血清进行鸡传染性法氏囊病抗体水平的监测是一种省力。省时有效,实用的抗体监测手段;同时,利用几种不同方法进行卵黄抗体的提取,结果聚乙二醇和氯仿处理获得的卵黄抗体提取液用于检测效果最好。 相似文献
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鸡白痢检疫多应用血清平板法(后称血平法)、琼脂扩散沉淀法(血琼法)。近年卵黄平板法(卵平法)和卵黄琼脂扩散沉淀法(卵琼法),也有应用于检疫的报道。但鸡白痢病卵黄平板法、琼扩法与血清平板法、琼扩法及卵黄平板法与琼扩法的符合率,尚未见研究报告。本试验进行了探讨,现报告如下。材料和方法1.试验材料(1) 试验鸡:460日龄鸡200只,选自数月前曾有鸡白痢临床发病的鸡群。(2) 试样采取:血样采自鸡翅翼根静脉,以针刺法刺破翅静脉,用2×3毫米高压聚乙烯输血管,长约10厘米,接取0.6毫升血液,待 相似文献
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鸡抗传染性法氏囊病卵黄抗体的ELISA及琼脂扩散检测 总被引：2，自引：0，他引：2
鸡传染性法氏囊病(IBD)是目前危害养鸡业的主要疾病之一。卵黄抗体(IgY)是母鸡血清中的IgG转移至卵黄中并积累形成，且与血清中抗体水平相当，因此通过检测卵黄抗体可监测鸡群的抗体水平，为鸡传染性疾病(如：IBD、新城疫、禽流感等)免疫程序的建立提供参考。研究使用间接ELISA(酶联免疫吸附试验)快速检测卵黄抗体的方法，同时对卵黄抗体的生产制备具有指导意义。 相似文献
10.
为明确鸡群经不同途径感染禽网状内皮组织增殖病病毒(REV)的排毒动态,本试验通过设立无特定病原体(SPF)鸡胚6胚龄卵黄囊感染组、1日龄SPF鸡腹腔感染组以及对上述两组SPF鸡分别加入感染鸡的卵黄囊感染同居组和腹腔感染同居组,建立SPF鸡感染REV模型,同时设置空白对照组,在不同日龄记录各组鸡的体重和死亡情况,并综合运用反转录PCR(RT-PCR)、实时荧光定量PCR(RT-qPCR)和间接免疫荧光试验(IFA)3种检测方法对各感染组进行REV的动态监测。结果显示：(1)与空白对照组相比,腹腔感染组和卵黄囊感染组SPF鸡体重增长在感染后第21～70天均受到显著抑制(P<0.05),卵黄囊感染组SPF鸡的死亡率在感染后第49和63天显著升高(P<0.05),腹腔感染组SPF鸡的死亡率在感染后第42天显著升高(P<0.05),卵黄囊感染组SPF鸡血浆中REV病毒载量在感染后第21天显著高于腹腔感染组(P<0.05);(2)卵黄囊感染组SPF鸡自出壳即可检测到病毒血症阳性,并在感染后第21天时达到排毒高峰,腹腔感染组SPF鸡在感染后第14天时达到排毒高峰;(3)卵黄囊... 相似文献
11.
抗鸡新城疫卵黄抗体IgY的研制 总被引：7，自引：2，他引：7
产蛋母鸡经新城疫灭活抗原重复免疫后，所产生的高免卵黄中，特异性抗体的HI效价可达到12log2以上。从高免卵黄中分离提纯的特异性新城疫抗体，病毒中和试验结果表明，具有明显的中和病毒的作用，特异性病毒中和指数可达到630957以上。使用纯化后的高免卵黄抗体(IgY)安全、高效、无任何不良反应。 相似文献
12.
Yolk harvested from eggs laid by hens hyperimmunized with killed Newcastle disease virus (NDV) was inoculated into the yolk sac of 1-day-old specific-pathogen-free (SPF) chickens. Serum hemagglutination-inhibition antibody titers reached maximum levels 1 to 4 days after yolk inoculation and declined at a rate similar to that reported for naturally acquired maternal antibody. Expected levels of immune interference were observed when yolk-inoculated chickens were vaccinated with a conventional oil-emulsion NDV vaccine. These results show that yolk-sac inoculation with yolk antibody is a suitable approach for producing maternally immune chickens for laboratory studies. 相似文献
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抗鸡新城疫和传染性法氏囊病二联卵黄抗体的研制及应用 总被引：2，自引：1，他引：1
用新城疫疫苗和传染性法氏囊病疫苗免疫健康高产蛋鸡制备抗鸡新城疫和传染性法氏囊病二联卵黄抗体,并在40个规模化肉鸡场做推广应用,结果表明,预防保护率达100%,治愈率为63%~85%。 相似文献
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非典型鸡新城疫的病因研究初报（一） 总被引：7，自引：1，他引：6
应用血清学、生物学方法、从我省产蛋量下降、HI抗体在1：128以上的疑似非典型鸡新城疫（CND）病鸡群中分离到1株病毒，经鉴定和致病力测定，明确是1株ND强毒，毒力与F48E9相近，然后采用该株野毒感染不同抗体水平的3批蛋鸡及肉鸡，结果3批蛋鸡均是低抗体滴度组（1：10－1：40）发生了CND症状，感染后6－7天开始产蛋停止，经15－17天后才恢复产蛋，高抗体滴定组（1：80以上）无明显变化，无抗体对照组出现典型鸡新城疫变化，全部死亡；病毒感染后第6、10天监测HI抗体，结果低滴度组第6天达1：80－1：160，第10天达1：160－1：1280。试验结果表明，我们分离到的NDV，既可引起典型的ND，也可引起非典型的ND；高HI抗体蛋鸡产生非典型鸡新城疫的病因之一，是由于存在低抗体鸡群而发生了CND，其高HI抗体有竞争是由于强毒感染后产生的抗体。 相似文献
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Oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODN) have been proven to be immunoprotective in mouse models. However, little work has been conducted on in vivo immune responses in chicken with CpG ODN. The objective of this study was to investigate the immunoadjuvant effects of CpG ODN to Newcastle disease (ND) vaccine and its protective effects against ND virus in SPF chicken. In this report, the titre of serum IgG to ND vaccine and the proliferation of lymphocytes were monitored in SPF chickens. The results demonstrated that the above-mentioned immune responses were significantly stronger in chickens that received CpG ODN than in the birds that received only ND vaccine. Furthermore, ND vaccine plus CpG ODN protected SPF chicken from challenge with an otherwise lethal dose of ND virus. These data suggest that CpG ODN holds considerable promise as an adjuvant for future vaccines against ND virus. 相似文献
17.
Vaccination of chickens using raw rice coated with novel trehalose nano-organogels containing Newcastle disease (strain I-2) vaccine 总被引：1，自引：1，他引：0
P. N. Wambura 《Tropical animal health and production》2009,41(5):797-802
The formulation and evaluation of trehalose nano-organogels for storage and oral delivery of Newcastle disease (ND) strain
I-2 vaccine to chickens were carried out in this study. Trehalose sugar was blended with vegetable oil to form nano-organogels
where trehalose also acted as a stabilizer against thermal inactivation of I-2 ND virus. Results from infectivity titration
assay indicated that the titre of 107.5 EID50/0.1 mL was maintained after 12 weeks of storage of nano-organogel I-2 vaccine at ambient room temperature. Serology results
showed that 33% chickens which were vaccinated with nano-organogel I-2 vaccine after 14 days had HI antibody titres of ≥ 3.0 log2 with GMT of 2.3. Moreover, results showed 100% of chickens vaccinated with nano-organogel I-2 vaccine had the mean antibody
titres of 3.4 and 3.7 log2 at 21 and 28 days after vaccination, respectively. All vaccinated chickens (100%) survived the challenge of virulent ND virus
whereas all unvaccinated chickens succumbed to challenge and died of signs consistent with ND. The findings from this study
showed that the nano-organogel I-2 vaccine was stable at room temperature, safe and produced protective antibody response
in vaccinated chickens. Moreover the nano-organogel I-2 vaccine was used for oral administration and hence is suitable for
mass vaccination. However, optimization of the formulation of trehalose nano-organogel vaccine is required in order to achieve
its application potentials. 相似文献
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The immunosuppressive effect of infectious bursal disease virus (IBDV) on vaccination against Newcastle disease (ND) was compared among 2-, 3-, and 4-week-old chickens inoculated with the highly virulent IBDV field isolate 90-11 and the reference serotype 1 strain GBF-1. In all age groups, isolate 90-11 severely suppressed antibody response to ND vaccination and protective vaccinal immunity against ND. In contrast, chickens inoculated with strain GBF-1 and vaccinated with ND vaccine were well protected from the ND virus challenge. The mitogenic response to phytohemagglutinin of splenic lymphocytes from chickens inoculated with isolate 90-11 or strain GBF-1 was significantly lower than that of uninoculated controls. There was no difference between the two inoculated groups in responsiveness, although lymphocyte depletion in the thymus was more severe in chickens inoculated with isolate 90-11 than in chickens inoculated with strain GBF-1. 相似文献
20.
Otim Onapa M Christensen H Mukiibi GM Bisgaard M 《Tropical animal health and production》2006,38(4):285-289
The role of ducks in the transmission of Newcastle disease virus (NDV) to free-range village chicken was investigated experimentally.
Newcastle disease (ND) seronegative ducklings reared in a pen were infected oronasally with velogenic NDV of intracerebral
pathogenicity index (ICPI) 1.8 isolated from outbreaks in village chickens in Uganda. A first group of 3-week-old ND seronegative
chicks was mixed with the ducks and they were kept together for 7 days. Both ducks and chicks were observed for ND clinical
signs and any mortality, and they were bled and their sera were tested for ND antibodies by haemagglutination inhibition (HI)
test. The chicks were removed, euthanized and examined for any ND lesions, while the ducks were transferred to a fresh pen
and a second group of chicks was introduced and observed and treated as above. The ducks and the chicks tested positive for
ND antibodies 7 days post infection and contact, respectively, but showed no clinical signs, post-mortem lesions or mortality.
The mean ND antibody titre of the second group of chicks was lower than for the first group. This study has shown that although
ducks can be infected with velogenic NDV, they do not show clinical signs but are able to transmit NDV to in-contact chicks.
Further investigations are needed of the lack of clinical signs in the in-contact chicks and how long the ducks remain infective. 相似文献