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1.
Endophytic bacteria carrying out dinitrogen (N2) fixation and indole acetic acid (IAA) synthesis were firstly identified in C. alismatifolia, a globally important flower crop. Their potential as growth promoters to stimulate the rapid growth of host plant was also examined. It will be beneficial to reduce the propagation period of tissue culture plantlets, and also utilize as a biofertilizer for rhizome production in the field. Seven endophytic bacteria were isolated from the leaf, four isolates from the leaf base, and two from the rhizome. ECS203, a gram-negative bacterium with a round shape, showed the highest N2 fixation at 4.2 nmol C2H4/106 cells/hr, and ECS202 showed the highest IAA synthesis at 296 μL μg ? 1 protein. Three selected isolates of N2-fixing and IAA synthesizing endophytic bacteria, i.e., ECS202, ECS203, and ECS204, isolated from the leaf base, were used to reinoculate Curcuma plantlets derived from tissue culture. Then, plants were grown in sterilized sand for 2 months and weekly supplied with N-free nutrient solution. Plant growth, colonization, nitrogen fixation, and IAA synthesis were measured at two months after planting. The inoculated plants clearly showed a better performance of plant growth and yield in terms of the plant height, plant weight, leaf area, and diameter of new rhizomes compared with uninoculated plants. The chlorophyll content and N concentration of leaves and roots also increased in inoculated plants. Endophytic bacteria from inoculated plants colonized the roots, rhizome, and leaf base. Partial sequence analysis using 16S rDNA indicated that the isolate ECS202 corresponded to Sphingomonas pseudosanguinis (99.2% similarity over 1,371 bp), ECS203 to Bacillus drentensis (99.4% similarity over 1,450 bp) and ECS204 to Bacillus methylotrophicus (99.9% similarity over 13,06 bp).  相似文献   

2.
A large number of actinomycetes (hundreds, thousands, and millions of CFU/g of soil) were isolated from saline soils in the territory of the landscapes of the Ber hillocks. A significantly smaller quantity of actinomycetes was isolated from the soda saline soils and sor saline soils that formed at the bottom of the ephemeral salty lakes in Buryatiya and in the estuary of the Syr-Darya River. Actinomycetes were represented in the studied soils by the Streptomyces, Micromonospora, Actinomadura, and Nocardiopsis genera. Among streptomycetes, the species of the Albus section and the Albus series predominated. The activity of the consumption of substrates by cultures of actinomycetes was largely influenced by conditions of preincubation. Obviously, the influence was related to the alteration in the metabolism of actinomycetes as one of the mechanisms of adaptation to the increased osmolarity of environment. The alcalotolerance, thermotolerance, and xerotolerance of halotolerant actinomycetes of the saline soils of arid territories were experimentally revealed.  相似文献   

3.
Abstract

Nutrient sorption studies were carried out as a part of a mega project on “Yield maximization in cassava (Manihot esculenta Crantz) through systematic approach in fertilizer use” to find out the sorption characteristics of major, secondary, and micronutrients [i.e., phosphorus (P), potassium (K), sulfur (S), copper (Cu), zinc (Zn), manganese (Mn), and boron (B)] in a typic kandiustult of Kerala, India. The sorption curve fitted by using this study along with the results of preliminary soil analysis and critical level of these nutrients were used to optimize the nutritional status of this soil. Sorption study revealed that P, K, S, and B were limiting and others were sufficient for this soil. The quantities of the limiting nutrients required to optimize the soil nutritional status were estimated from the sorption curve as P, K, S, and B at 136 µg mL?1, 0.338 meq 100 mL?1, 20 µg mL?1, and 6.025 µg mL?1, respectively.  相似文献   

4.
A commonly occurring auxin indole‐3‐aceticacid (IAA) and a rarely occurring chlorosubstituted auxin (4‐Cl‐IAA) were compared for their impact on growth and nitrogen metabolism in mung bean for the first time. The plants were generated from healthy and Rhizobium coated seeds in earthen pots. The seedlings at 7 and/or 14 days were percolated with 0, 10?10, 10?8, or 10?6 M of IAA or 4‐Cl‐IAA. The plants were sampled at 30 days after sowing (DAS) to assess the growth and various biochemical characteristics. The auxins significantly enhanced the growth (length and dry mass of shoot and root), nodule fresh mass, nitrogenase activity in fresh nodules, leaf carbonic anhydrase activity, chlorophyll content, and rate of photosynthesis. The effect of the auxins lasted up to the harvest where the seed yield, 100 seed mass, and number of pods per plant were significantly affected by the auxins. At a moderate concentration (10?8 M), 4‐Cl‐IAA generated the best response. However, a comparable response was generated by the higher concentration (10?6 M) of 4‐Cl‐IAA. The application of the hormone twice (at 7 and 14 DAS) was much more effective than single application (at 7 or 14 DAS). It was concluded that IAA and 4‐Cl‐IAA improved the growth and nitrogen fixation in mung bean. The 4‐Cl‐IAA proved more effective than IAA.  相似文献   

5.
Fifty strains of bacteria isolated from banana roots were studied for their plant growth promoting (PGP) activities. Indole -3- acetic acid (IAA) production by root-associated bacteria ranged from 20 to 302 µg ml?1. Seventeen isolates (34%) were positive for siderophore production and 18 isolates (36%) showed phosphate solubilization. None of the isolates showed potassium solubilization. All the isolates showed growth on nitrogen free Jensen medium. Identification of the bacteria based on 16S rRNA gene sequencing revealed that the isolates belonged to genus Bacillus sp, Klebsiella sp, Microbacterium sp and Enterobacter sp. A pot experiment in a greenhouse was conducted to investigate the effect PGP bacteria on banana plant growth and enzyme activities. The results demonstrated a significant (P < 0.05) increase in plant growth, chlorophyll, total phenolics, proline, catalase and ascorbic acid oxidase in banana plants treated with PGP bacteria as compared to control. However, the plant-growth response was variable and dependent on the bacterial strains, enzyme activity, and growth parameter observed. The present study revealed that bacteria showing multiple PGP activity could be used as biostimulants in enhancing banana production.  相似文献   

6.
In order to study the potential use of microfauna as an indicator of effluent quality and operational parameters in an activated sludge system for treating piggery wastewater, an experimental sequencing batch reactor was set up and evaluated by biological and physical–chemical analyses for 12 months. Results show that microfauna (and specifically ciliate protozoa) are a good parameter for assessing effluent quality in terms of both chemical oxygen demand (COD) and ammonia and for assessing the organic and nitrogen load of the system. Specifically, the abundance of ciliates decreases from 20,000 individuals·mL?1 to ca. 2,500 individuals·mL?1 and from ca. 10,000 individuals mL?1 to ca. 200 individuals mL?1 when effluent concentration is between 550 and 750 mg L?1 and above 100 mg L?1 to the COD and ammonia concentrations, respectively. Furthermore, microfauna abundance is reduced from ca. 18,000 individuals mL?1 (organic load between 0.1 and 0.2 mg COD mg total suspended solids (TSS)?1 day?1) to ca. 500 individuals mL?1 (organic load between 0.3 and 04 mg COD mg TSS?1 day?1). Microfauna abundance also decreases as nitrogen loading increases. Nitrogen loading in the range of 5–60 mg NH4–N g TSS?1 day?1 does not have any significant effect on microfauna abundance. However, ammonia loading from 60 to 120 mg NH4–N g TSS?1 day?1 reduces microfauna abundance ca. 6-fold. Ciliate protozoa were the largest microfauna group during the whole period of study, representing ca. 75% of the total microfauna abundance. The largest group in the ciliate community was that of the free-swimming ciliates. This was followed by the group of attached and crawling ciliates. Specifically, the dominant ciliate species during the whole study period were Uronema nigricans, Vorticella microstoma-complex, Epistylis coronata, and Acineria uncinata.  相似文献   

7.
In this work, the relationship among accumulation of selenium, auxin, and some nutrient elements [magnesium (Mg2+), iron (Fe3+), manganese (Mn2+), copper (Cu2+), zinc (Zn2+)] in tissues of roots, mesocotyls, and leaves of Zea mays L. plants was studied. Seeds of maize were cultivated for 4 days in the darkness at 27 °C on moist filter paper, then the individual seedlings were transferred into an aerated solution containing the macro‐ and microelements and were cultivated in a greenhouse for 12 h in the light and 12 h (12‐h photoperiod) in the dark at 25 °C. The seedlings were exposed to the solution containing sodium hydrogen selenite (NaHSeO3), indole‐3 acetic acid (IAA), or IAA+NaHSeO3 for approximately 96 h before chemical analysis. The concentration of IAA in the external medium was 10?4 mol dm?3, concentration of selenite (NaHSeO3) was 10?6 mol dm?3, and the pH of the medium was 6.5.

The accumulation of the probed elements in seedlings of maize was measured by inductively coupled plasma optical emission spectroscopy (ICP‐OES). It was determined that the selenite and IAA, present in the external medium of growing plants, changed the uptake and accumulation of some cations in tissues of leaves, mesocotyls, and roots. The change of transport conditions of these nutrient elements is probably one of the first observed symptoms of selenium effects on plants.  相似文献   

8.
Abstract

Maximum yield research (MYR) and maximum economic yield systems (MEY) are important under the present situation of increased demographic changes, which have reduced the per capita availability of both land and food. Among root and tuber crops, cassava is found in a variety of production systems and performs well under various levels of management from low‐input to high‐input systems. A systematic approach in fertilizer use involves the determination and elimination of soil nutrient constraints for balanced supply of all potentially deficient essential nutrients for sustainable high yield. In the systematic approach of determining optimal fertilizer for cassava (Manihot esculenta Crantz) in a Typic Kandiustult soil of Kerala, India, studies on critical levels of nutrients, original nutritional status of the soil, its sorption capacity, and greenhouse/screenhouse nutrient survey were carried out. The critical levels of phosphorus (P) and potassium (K) were determined as 8.23 and 43.5 µg g?1, respectively. The preliminary analysis of the soil of the experimental site revealed the overall fertility status as very poor. The sorption studies conducted for P, K, copper (Cu), zinc (Zn), manganese (Mn), sulfur (S), and boron (B) indicated P, K, S, and B were limiting in this particular soil. The quantities of these nutrients needed to optimize the soil nutrient status were determined from the preliminary soil analysis, critical levels of nutrients, and sorption curves and found to be 136 µg mL?1 P, 0.338 meq 100 mL?1 K, 20 µg mL?1 S, and 6.025 µg mL?1 B, respectively. From the greenhouse nutrient survey, N, P, K, and calcium (Ca) were seen as limiting nutrients for this soil; therefore the optimum treatment for cassava was fixed as N‐P‐K at 100:300:300 kg ha?1, respectively. Field experiments were conducted for two seasons with different levels of the optimum treatment using a short duration (6–7 months) cassava variety, Sree Vijaya. The optimum treatment gave a yield of 43.41 t ha?1, whereas the yield under the existing practice recommendations was 20–25 t ha?1. Economic analysis showed this optimum treatment as the best in terms of highest gross return (Rs 1,31,125/‐), net return (Rs 83,150/‐), added return (Rs 70, 178/‐), added profit (Rs 56,142/‐), and benefit cost ratio (BCR) (2.73).  相似文献   

9.
Plant growth promoting bacteria (PGPB) are known to colonize ginger rhizome. A total of 57 strains were isolated and identified by 16S rDNA PCR-RFLP fingerprinting, and were grouped into genera Serratia, Enterobacter, Acinetobacter, Pseudomonas, Stenotrophomonas, Agrobacterium, Ochrobactrum, Bacillus and Tetrathiobacter. Out of 34 representative strains, 14 were positive for indole-3-acetic acid (IAA) production (1.02–49.66 µg ml–1), 16 were able to solubilize phosphate (9.3–233.05 µg ml–1) and 18 showed siderophore activities (9.47–70.66%). Six representative strains with plant growth promoting (PGP) activities boosted the growth of Zea mays, where not only the plant height, leaf area, and biomass yield were significantly improved, plant N, P and K uptakes were also increased. Plants co-inoculated with both PGPB and chemical fertilizer also showed an increased yield. Our study thus indicates that there is an abundance of endophytic bacteria in ginger rhizome, some of which possess promising capabilities for enhancing the growth of Z. mays and have the potential to serve as bio-fertilizers for crops.  相似文献   

10.
The research goal was to determine if onsite wastewater system (OWS) density had an influence on the concentrations and watershed exports of Escherichia coli and enterococci in urbanizing watersheds. Eight watersheds with OWS densities ranging from <?0.1 to 1.88 systems ha?1 plus a watershed served by sewer (Sewer) and a mostly forested, natural watershed (Natural) in the Piedmont of North Carolina served as the study locations. Stream samples were collected approximately monthly during baseflow conditions between January 2015 and December 2016 (n?=?21). Median concentrations of E. coli (2014 most probable number (MPN) 100 mL?1) and enterococci (168 MPN 100 mL?1) were elevated in streams draining watersheds with a high density of OWS (>?0.77 system ha?1) relative to watersheds with a low (<?0.77 system ha?1) density (E. coli: 204 MPN 100 mL?1 and enterococci: 88 MPN 100 mL?1) and control watersheds (Natural: E. coli: 355 MPN 100 mL?1 and enterococci: 62 MPN 100 mL?1; Sewer: 177 MPN 100 mL?1 and 130 MPN 100 mL?1). Samples collected from watersheds with a high density of OWS had E. coli and enterococci concentrations that exceeded recommended thresholds 88 and 57% of times sampled, respectively. Results show that stream E. coli and enterococci concentrations and exports are influenced by the density of OWS in urbanizing watersheds. Cost share programs to help finance OWS repairs and maintenance are suggested to help improve water quality in watersheds with OWS.  相似文献   

11.
Abstract

Viruses are the most abundant biological entities in marine and freshwater environments. Many studies have shown the ecological importance of viruses in the primary production and microbial food web in aquatic environments. However, no studies have examined viral abundance in the floodwater of paddy fields. The present study surveyed the abundance of virus-like particles (VLPs) and bacteria in the floodwater of a Japanese paddy field under a long-term fertilizer trial since 1925 during the rice cultivation period. Virus-like particles and bacterial abundances in the floodwater ranged from 5.6 × 106 to 1.2 × 109 VLPs mL?1 and from 9.2 × 105 to 4.3 × 108 cells mL?1 with mean abundances of 1.5 × 108 VLPs mL?1 and 5.1 × 107 cells mL?1, respectively, and increased with an increase in the turbidity of the floodwater with suspended particles. The magnitude of seasonal variation was more than 50-fold for VLP abundance and 100-fold for bacterial abundance. The virus-to-bacterium ratios fluctuated over the rice cultivation period, ranging from 0.11 to 72 and their increase correlated with the decrease in bacterial abundance. Our results suggest that viral abundance in the floodwater of paddy fields is larger than in natural marine and freshwater environments.  相似文献   

12.
In the present study, a method based on dispersive liquid–liquid microextraction (DLLME) followed by graphite furnace atomic absorption spectrometry (GFAAS) determination was proposed for the determination of selenium by using ammonium pyrrolidine dithiocarbamate (APDC) as the chelating reagent. The main factors influencing the DLLME were investigated systematically. Under the optimal conditions, the limit of detection for Se(IV) was 0.02 ng mL?1. The relative standard deviation was 4.1% (CSe(IV) = 0.2 ng mL?1, n = 8) with an enhancement factor of 135.8-fold from only 5 mL of the water sample. The proposed method was successfully applied to the determination of Se(IV) in anodic slime and electrolyte samples. In order to validate the proposed method, a Certified Reference Material (trace elements in water, 1643e, NIST) was analyzed, and the determined value obtained was in good agreement with the certified value.  相似文献   

13.
A simple and very sensitive spectrophotometric method determining microgram quantities of gallium (Ga) has been developed. The method is based on the complex of Ga(III) with Chrome Azurol S (CAS) in the presence of benzyldodecyldimethylammonium bromide (ST) and polyoxyethylenesorbitan monopalmitate (Tween 40). The complex forms in weakly acid solution (pH 3.8) and shows the absorption maximum at 627 nm. The molar absorptivity (ε) of this system is 1.87 × 105 L mol?1 cm?1, and it is clearly higher than ε of other spectrophotometric methods of gallium determination. The detection and quantification limits (LOD, LOQ) are 7.6 × 10–3 and 0.023 µg mL?1, respectively. Beer‘s law is obeyed in the range from 0.02 to 0.60 µg mL?1 of Ga. The precision and accuracy are good. This first spectrophotometric method of Ga(III) determination in the four-component system has been successfully applied for the analysis of mushrooms and grasses with low content of gallium.  相似文献   

14.
The field experiment was conducted on black soil (Vertic Ustropept) at Zonal Agricultural Research Station farm, Solapur, for successive 30 years from 1987–1988 to 2016–2017 under dryland condition in a randomized block design with 10 treatments and 3 replications. The pooled results of seven years (2010–2011 to 2016–2017) revealed that the application of 25 kg N ha?1 through crop residue (CR, byre waste) along with 25 kg N ha-1 through Leucaena lopping (Leucaena leucocephala) to rabi sorghum gave significantly higher grain and stover yield and Sustainable Yield Index (14.61 and 36.11 q ha?1 and 0.47, respectively) which was on par with T7, where 25 kg N ha?1 through farmyard manure (FYM) + 25 kg N ha?1 through urea was applied for grain and stover yield (13.95 and 34.46 q ha?1 and 0.44, respectively). The gross and net monetary returns and benefit–cost ratio were also influenced significantly due to integrated nitrogen management (Rs. 59,796, Rs. 47,353 ha?1, and 3.13, respectively). This was also reflected in residual soil fertility status of soil after harvest of rabi sorghum. The organic carbon content and available nitrogen content of soil, as well as nitrogen uptake and moisture use efficiency for grain, were also increased. The total microbial count of bacteria, fungi, and actinomycetes was more where FYM or CR addition was done. The count of N fixers and P solubilizers was more under Leucaena application either alone or with CR or urea. Application of CR at 4.8 t ha?1 (25 kg N ha?1) along with Leucaena lopping at 3.5 t ha?1 (25 kg N ha?1) as green leaf manure is the best alternative organic source for fertilizer urea (50 kg N ha?1) to increase the production of dryland rabi sorghum.  相似文献   

15.
Abstract

Gram-positive bacterium BRF-1 isolated from the rhizosphere of root-rotten diseased soybean plants shows strong antifungal activity on plates. In the present study, the ability of BRF-1 to control brown stem rot disease and to promote plant growth was examined using soybean plants grown in pots. Brown stem rot is a severe soil-borne disease of soybean that is caused by the pathogenic fungus Phialophora gregata. Eighteen-day-old soybean seedlings were grown in a mixture of soil (40 mL) and BRF-1 suspension (10 mL) and were dipped in a suspension of P. gregata (106 c.f.u. mL?1), and the severity of the disease was judged 62 days after the fungal inoculation. Inoculation with BRF-1 at 107 and 108 c.f.u. mL?1 significantly lowered the severity of the disease. Soybean seeds incubated in 100 mL of the BRF-1 suspension (2 × 106 c.f.u. mL?1) were sown in pots filled with sand, and plants were collected 7 days after emergence. The dry weight increase in shoots and roots and mineral absorption were significantly stimulated by the inoculation. These results suggest that BRF-1 might be an effective biocontrol agent to manage brown stem rot disease in soybean. The 16S rDNA sequence of BRF-1 showed high homology with sequences of Paenibacillus polymyxa strains (e.g. 99.7% identity with P. polymyxa strain WY110).  相似文献   

16.
The population and taxonomy of actinomycetes in chestnut soils of Mongolian plains were studied. The actinomycetes identified belong to the genera Streptomyces, Micromonospora, Saccharopolyspotra, Streptosporangium, and Microbiospora. In the composition of Streptomyces, the species of sections Cinereus, Helvo-Flavus, Albus, and Imperfectus were present. The species of Imperfectus section predominated in the Streptomyces complex and amounted to 88.2%.  相似文献   

17.
Abstract

Nitrous oxide (N2O) emissions were measured monthly over 1 year in three ecosystems on tropical peatland of Sarawak, Malaysia, using a closed-chamber technique. The three ecosystems investigated were mixed peat swamp forest, sago (Metroxylon sagu) and oil palm (Elaeis guineensis) plantations. The highest annual N2O emissions were observed in the sago ecosystem with a production rate of 3.3 kg N ha?1 year?1, followed by the oil palm ecosystem at 1.2 kg N ha?1 year?1 and the forest ecosystem at 0.7 kg N ha?1 year?1. The N2O emissions ranged from –3.4 to 19.7 µg N m?2 h?1 for the forest ecosystem, from 1.0 to 176.3 µg N m?2 h?1 for the sago ecosystem and from 0.9 to 58.4 µg N m?2 h?1 for the oil palm ecosystem. Multiple regression analysis showed that N2O production in each ecosystem was regulated by different variables. The key factors influencing N2O emissions in the forest ecosystem were the water table and the NH+ 4 concentration at 25–50 cm, soil temperature at 5 cm and nitrate concentration at 0–25 cm in the sago ecosystem, and water-filled pore space, soil temperature at 5 cm and NH+ 4 concentrations at 0–25 cm in the oil palm ecosystem. R2 values for the above regression equations were 0.57, 0.63 and 0.48 for forest, sago and oil palm, respectively. The results suggest that the conversion of tropical peat swamp forest to agricultural crops, which causes substantial changes to the environment and soil properties, will significantly affect the exchange of N2O between the tropical peatland and the atmosphere. Thus, the estimation of net N2O production from tropical peatland for the global N2O budget should take into consideration ecosystem type.  相似文献   

18.
In this work, silver iodide (Ag(I)) ions were separated via the carrier element-free coprecipitation (CEFC) method using an organic coprecipitating agent, 1,6-diamino-4-(4-chlornphenyl)-2-oxo-1,2-dihydropyridine-3,5-dicarbonitrile [DCODD] prior to its determination by ?ame atomic absorption spectrometry (FAAS). Analytical parameters including pH of aqueous solution, amount of DCODD, standing time, centrifugation rate and time, and sample volume were studied and optimized. Under the best experimental conditions, it was found that extraction can be performed from the sample volume of 500.0 for silver ion (preconcentration factor of 100.0). Linearity was maintained between 0.006 and 1.50 µg.mL?1 for silver. Detection limit for silver based on 3Sb was 1.60 ng.mL?1. The relative standard deviation of eight replicate measurements of 0.20 µg.mL?1of silver was 2.10%. Finally, the developed method was successfully applied to extraction and determination of the silver ions in the Lepidium draba L plant, water and standard samples and satisfactory results were obtained.  相似文献   

19.
Enhancement of plant growth by Bacillus is well documented and several mechanisms have been suggested for the phytostimulatory activity of this group of plant growth-promoting rhizobacteria (PGPR). In the present work, the PGP potential of plant associated Bacillus spp. and their growth-promoting effect on wheat were studied. Six out of 35 strains were chosen based on seed germination assay, plant growth-promoting abilities, enzymatic function, and auxin production. All tested strains were subjected to pot experiments and their phenotypic and molecular assays were also done. Two Bacillus strains including WhIr-15 and WhIr-12 produce maximum amount of auxin (16.2 and 14 µg ml?1, respectively). Strain WhIr-15 had just the ability to produce indo-3-acetic acid (IAA), lipase, and protease enzymes. Strain WhIr-12 was also recorded positive for siderophore, auxin production, and phosphorus (P) solubilization. Bacterial IAA production positively correlated with root length (r = 0.875; p ≤ 0.05). Significant enhancement in root weight (71% and 53%) and in panicle weight (91% and 77%) was recorded in WhIr-15 and WhIr-12, respectively, over untreated controls. Based on phenotypic and 16S rDNA sequencing, these two strains belong to Bacillus sp. Based on our results, phytohormone-producing Bacillus sp. can be applied at field level to improve wheat productivity.  相似文献   

20.
The contamination of hazardous metal(loid) is one of the serious environmental and human health risks. This study isolated a total of 40 cadmium (Cd)- and arsenic (As)-resistant bacterial isolates from coastal sediments by pour plate technique using tryptic soy agar supplemented with Cd or As (50 mg l?1) for use as metal(loid) bioremediation agents. Out of 40, 4 isolates, RCd3, RCd6, RAs7, and RAs10, showed a relatively higher growth rate in Cd- or As-supplemented culture media which were selected for further study. The selected isolates showed a high minimum inhibitory concentration (60–400 mg l?1 for Cd and 400–2200 mg l?1 for As), which demonstrated their remarkable Cd and As resistance capabilities. The metal(loid) removal efficiencies (0.032–0.268 μg Cd h?1 mg?1 and 0.0003–0.0172 μg As h?1 mg?1 [wet weight cell]) of selected isolates indicated their greater magnitude in absorbing Cd compared to As from water. Phylogenetic analysis of the 16S rDNA sequences revealed that isolates RCd3, RCd6, RAs7, and RAs10 were closely related to Acinetobacter brisouii, Pseudomonas abietaniphila, Exiguobacterium aestuarii, and Planococcus rifietoensis, respectively. Because of high Cd and As resistance and removal efficiency, the selected isolates can survive in a high metal(loid)-contaminated environment and could be a potential tool for bioremediation of high metal(loid)-contaminated effluents to protect the aquatic environment.  相似文献   

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