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1.
Shiv Shankhar Kaundun 《Pest management science》2010,66(11):1249-1256
BACKGROUND: The increasing use of ACCase‐inhibiting herbicides has resulted in evolved resistance in key grass weeds infesting cereal cropping systems worldwide. Here, a thorough and systematic approach is proposed to elucidate the basis of resistance to three ACCase herbicides in a Lolium multiflorum Lam. (Italian rye grass) population from the United Kingdom (UK24). RESULTS: Resistance to sethoxydim and pinoxaden was always associated with a dominant D2078G (Alopecurus myosuroides Huds. equivalent) target‐site mutation in UK24. Conversely, whole‐plant herbicide assays on predetermined ACCase genotypes showed very high levels of resistance to diclofop‐methyl for all three wild DD2078 and mutant DG2078 and GG2078 ACCase genotypes from the mixed resistant population UK24. This indicates the presence of other diclofop‐methyl‐specific resistance mechanism(s) yet to be determined in this population. The D2078G mutation could be detected using an unambiguous DNA‐based dCAPS procedure that proved very transferable to A. myosuroides, Avena fatua L., Setaria viridis (L.) Beauv. and Phalaris minor Retz. CONCLUSION: This study provides further understanding of the molecular basis of resistance to ACCase inhibitor herbicides in a Lolium population and a widely applicable PCR‐based method for monitoring the D2078G target‐site resistance mutation in five major grass weed species. Copyright © 2010 Society of Chemical Industry 相似文献
2.
Vulpia myuros is an annual grass species of Mediterranean origin, which has achieved a global distribution. It is a fast‐growing species, with high colonisation and competitive abilities. This species is considered an invasive weed in most countries where it has been introduced, with highly negative economic impact where it now dominates. It is increasingly found to be a problematic weed in winter cereals, especially in no‐till and reduced tillage systems, across Europe, United States and Australia. Seeds of V. myuros have reduced germination potential when buried. However, where tillage interventions are reduced, ideal conditions for V. myuros are created. Minimum and no tillage practices are increasing worldwide, with a concomitant increase in the spread and abundance of V. myuros. Effectiveness of herbicides is mostly suboptimal, in particular for well‐established populations forming dense swards, even though no herbicide resistance has yet been identified. An integrated management approach, increasing crop diversification combined with management adaptations, possibly including herbicides is suggested as an effective control strategy. Despite increasing research on V. myuros, more information is needed to optimise the management of this weed. Based on the species’ Mediterranean origins and adaptation to warm and dry environments, an increase of its global importance may be expected with climate changes. It is thus paramount to increase the awareness around this species, improve its identification in the field and monitor its spread before it becomes a concern of similar magnitude to grass weeds like Alopecurus myosuroides or Lolium rigidum. 相似文献
3.
Lolium rigidum (annual or rigid ryegrass) is a widespread annual weed in cropping systems of southern Australia, and herbicide resistance in L. rigidum is a common problem in this region. In 2010, a random survey was conducted across the grain belt of Western Australia to determine the frequency of herbicide‐resistant L. rigidum populations and to compare this with the results of previous surveys in 1998 and 2003. During the survey, 466 cropping fields were visited, with a total of 362 L. rigidum populations collected. Screening of these populations with the herbicides commonly used for control of L. rigidum revealed that resistance to the ACCase‐ and ALS‐inhibiting herbicides was common, with 96% of populations having plants resistant to the ACCase herbicide diclofop‐methyl and 98% having plants resistant to the ALS herbicide sulfometuron. Resistance to another ACCase herbicide, clethodim, is increasing, with 65% of populations now containing resistant plants. Resistance to other herbicide modes of action was significantly lower, with 27% of populations containing plants with resistance to the pre‐emergent herbicide trifluralin, and glyphosate, atrazine and paraquat providing good control of most of the populations screened in this survey. Ninety five per cent of L. rigidum populations contained plants with resistance to at least two herbicide modes of action. These results demonstrate that resistance levels have increased dramatically for the ACCase‐ and ALS‐inhibiting herbicides since the last survey in 2003 (>95% vs. 70–90%); therefore, the use of a wide range of integrated weed management options are required to sustain these cropping systems in the future. 相似文献
4.
杂草对ACCase抑制剂的抗性 总被引：3，自引：0，他引：3
以乙酰辅酶A羧化酶（ACCase）为作用靶标的除草剂是高效、选择性的禾本科杂草除草剂,其在全球范围内的广泛、重复使用,导致了抗药性杂草的发生和发展。到目前为止,已经在30个国家有37种抗此类除草剂的杂草生物型。抗药性杂草严重威胁杂草治理和农业生产,由此引发的生态问题及粮食安全问题引起了广泛的关注。文章概述了ACCase抑制剂抗药性杂草的发生现状,从杂草ACCase突变、代谢解毒等几个方面综述了杂草抗ACCase抑制剂的抗性机制,以期为ACCase抑制剂抗性研究提供参考。最后讨论了阻止或延缓抗药性发生的杂草管理措施。 相似文献
5.
Substitution of isoleucine by leucine at the equivalent of residue 1781 of acetyl CoA carboxylase (ACCase) in Alopecurus myosuroides (I1781L) has been shown to be a key point mutation conferring resistance to most aryloxypropionate and cyclohexanedione herbicides in Lolium spp., A. myosuroides, Avena fatua and Setaria viridis. This substitution results from changing an adenine residue to either thymine or cytosine at position 5341 in the ACCase coding sequence of A. myosuroides and at the homologous position in the other species. The I1781L mutation can be detected by allele‐specific amplification assays. These are, however, very dependent on the conservation of the nucleotide sequences flanking the causative single nucleotide polymorphism. Moreover, such assays cannot distinguish between homozygous and heterozygous individuals in a single polymerase chain reaction reaction. Here we present an alternative derived Cleaved Amplified Polymorphic Sequence (dCAPS) method to define I1781L status in the ACCase enzyme of four grass weeds. This dCAPS approach is simple, economical, highly transferable between species and can readily distinguish homozygous Leu/Leu 1781 and heterozygous Ile/Leu 1781 resistant individuals, providing the basis for accurate measures of the frequency of the dominant Leu allele in a given population. 相似文献
6.
Michaela Kolářová Theresa A. Reinhardt Piskáčková Luděk Tyšer Thien T. Hoová 《Weed Research》2023,63(1):57-67
Herbicide resistance is a widespread issue that impacts management of conventional farms, but also has ramifications for the weed community assembly; it is therefore important to see how these species factor into the weed community assembly of farms throughout the countryside. This research analysed species richness and community diversity in 98 field relevés from 48 organic and 50 conventional farms evenly distributed between two major production regions of the Czech Republic and then evaluated the incidence of species which have been reported resistant in the Czech Republic and its neighbouring countries. Farms were selected independently of any acknowledged resistant species. Out of 164 species found in this survey, only eight species have had herbicide-resistant biotypes reported in the Czech Republic, while a total of 19 species had herbicide-resistant biotypes reported in neighbouring countries. Species with recorded resistance to PSII inhibitors in the Czech Republic tended to be found together and were mostly associated with the beet production region, characterised by low altitude: Amaranthus retroflexus, Chenopodium album, Echinochloa crus-galli and Solanum nigrum. Species with reported resistance to ALS and ACCase-inhibiting herbicides were not clearly associated with a particular region or farming type. Of the species which have had reported herbicide resistance in the neighbouring countries, several were found in conventional fields within the growing season and we recommend immediate screening for herbicide resistance in these species and more diligent action in management according to anti-resistance strategies: Bromus sterilis resistant to ACCase-inhibiting herbicides, A. retroflexus resistant to ALS-inhibiting herbicides or Avena fatua resistant to ACCase and ALS-inhibiting herbicides. This work is unique in that it is evaluating weed species diversity in organic and conventional farms and using the context to frame the prevalence of high-risk herbicide-resistant species; thereby putting the potential incidence of herbicide resistance into perspective at the landscape level. 相似文献
7.
Debrah F Lorraine-Colwill Tim R Hawkes Patricia H Williams Simon AJ Warner Peter B Sutton Stephen B Powles Christopher Preston 《Pest management science》1999,55(4):489-491
Annual ryegrass (Lolium rigidum) is a widespread and important weed of Australia and populations of this weed have developed resistance to most major herbicides, including glyphosate. The possible mechanisms of resistance have been examined in one glyphosate-resistant Lolium population. No major differences were observed between resistant and susceptible biotypes in respect of (i) the target enzyme (EPSP synthase), (ii) DAHP synthase, the first enzyme of the target (shikimate) pathway, (iii) absorption of glyphosate, or (iv) translocation. Following treatment with glyphosate, there was greater accumulation of shikimate (derived from shikimate-3-Pi) in susceptible than in resistant plants. In addition, the resistant population exhibited cross-resistance to 2-hydroxy-3-(1,2,4-triazol-1-yl)propyl phosphonate, a herbicide which, although structurally similar to glyphosate, acts at an unrelated target site. On the basis of these observations we speculate that movement of glyphosate to its site of action in the plastid is involved in the resistance mechanism. © 1999 Society of Chemical Industry 相似文献
8.
JM Green 《Pest management science》2012,68(10):1323-1331
Since 1996, genetically modified herbicide-resistant crops, primarily glyphosate-resistant soybean, corn, cotton and canola, have helped to revolutionize weed management and have become an important tool in crop production practices. Glyphosate-resistant crops have enabled the implementation of weed management practices that have improved yield and profitability while better protecting the environment. Growers have recognized their benefits and have made glyphosate-resistant crops the most rapidly adopted technology in the history of agriculture. Weed management systems with glyphosate-resistant crops have often relied on glyphosate alone, have been easy to use and have been effective, economical and more environmentally friendly than the systems they have replaced. Glyphosate has worked extremely well in controlling weeds in glyphosate-resistant crops for more than a decade, but some key weeds have evolved resistance, and using glyphosate alone has proved unsustainable. Now, growers need to renew their weed management practices and use glyphosate with other cultural, mechanical and herbicide options in integrated systems. New multiple-herbicide-resistant crops with resistance to glyphosate and other herbicides will expand the utility of existing herbicide technologies and will be an important component of future weed management systems that help to sustain the current benefits of high-efficiency and high-production agriculture. Copyright © 2012 Society of Chemical Industry 相似文献
9.
Differences in the molecular basis of resistance to the cyclohexanedione herbicide sethoxydim in Lolium multiflorum 总被引：1，自引：0，他引：1
Resistance to the cyclohexanedione (CHD) herbicide sethoxydim was investigated in two UK Lolium multiflorum populations, Yorks A2 and PYL. Resistance screening experiments demonstrated a qualitative difference in the responses of the two populations to sethoxydim, suggesting that the molecular basis of resistance between them was different. After treatment, Yorks A2 plants were either alive (78% of sample tested) or dead (22% tested) but plants of the PYL population showed two further intermediate categories of response. The level of acetyl-CoA carboxylase (ACCase) insensitivity directly correlated with the degree of resistance at the whole plant level, indicating that the molecular basis of resistance is associated with differences in ACCase sensitivity in each population. Direct sequencing of the carboxyl transferase domain of the ACCase gene showed that an Ile-418-Leu substitution in the L. multiflorum chloroplastic ACCase (GenBank accession number AY710293 ) confers resistance to sethoxydim in Yorks A2. This corresponds to amino acid residue 1781 in the Alopecurus myosuroides full ACCase sequence. This is the first report of this mutation in this L. multiflorum, which has also been reported in four other grass-weeds, including L. rigidum. However, no amino acid substitutions were found to be specifically associated with the resistant phenotypes in the PYL population and the molecular basis of resistance in this population remains to be resolved. 相似文献
10.
小麦是我国主要的粮食作物, 麦田草害的发生与危害严重影响小麦的产量与品质。我国小麦田的禾本科杂草主要包括日本看麦娘、看麦娘、菵草和多花黑麦草等, 田间杂草防除主要依靠化学除草剂。精噁唑禾草灵自20世纪80年代进入我国市场以来, 长期用于小麦田禾本科杂草防除, 已经报道麦田多种禾本科杂草对精噁唑禾草灵产生了高水平抗药性。为更加科学合理地控制小麦田杂草的发生与危害, 一些学者就麦田禾本科杂草对精噁唑禾草灵的抗药性水平监测和抗性机理进行了系统研究。本文系统总结了我国小麦田抗精噁唑禾草灵杂草的发生种群、抗性水平、靶标酶抗性机理与非靶标抗性机理, 还梳理了抗性杂草的交互抗性与多抗性发生情况, 分析了我国在抗性杂草治理方面的经验以及面临的问题, 为农田杂草防除提供指导。 相似文献
11.
12.
'Universal' primers for PCR-sequencing of grass chloroplastic acetyl-CoA carboxylase domains involved in resistance to herbicides 总被引：2，自引：0，他引：2
Primers were designed to amplify two regions involved in sensitivity to herbicides inhibiting the plastidic acetyl-CoA carboxylase (ACCase) from grasses (Poaceae). The first primer pair amplified a 551-bp amplicon containing a variable Ile/Leu codon at position 1781 in Alopecurus myosuroides sequence. The second primer pair amplified a 406-bp amplicon containing four variable codons (Trp/Cys, Ile/Asn, Asp/Gly, Gly/Ala) at positions 2027, 2041, 2078 and 2096, respectively, in A. myosuroides sequence. Both primer pairs amplified the targeted fragments from genes encoding plastidic ACCases, but not from the very similar genes encoding cytosolic ACCases. Clear DNA sequences were obtained from fresh or dried plant material from the field, and from 29 various grass species. Sequences revealed that the gene encoding plastidic ACCase in Poa annua and Festuca rubra contained a Leu1781 codon, in agreement with both species being inherently tolerant to herbicides inhibiting ACCase. Sequencing confirmed the hybrid origin of P. annua. Compared with ACCase enzyme assay, polymerase chain reaction is faster, can be performed from a single plant and suppresses the need for radioactive experiments. It can be completed with basic molecular biology laboratory equipment. It is the tool of choice for diagnosing resistance caused by alteration(s) of the plastidic ACCase. 相似文献
13.
Taking stock of herbicide-resistant crops ten years after introduction 总被引：11，自引：0，他引：11
Duke SO 《Pest management science》2005,61(3):211-218
Since transgenic, bromoxynil-resistant cotton and glufosinate-resistant canola were introduced in 1995, planting of transgenic herbicide-resistant crops has grown substantially, revolutionizing weed management where they have been available. Before 1995, several commercial herbicide-resistant crops were produced by biotechnology through selection for resistance in tissue culture. However, non-transgenic herbicide-resistant crops have had less commercial impact. Since the introduction of glyphosate-resistant soybean in 1996, and the subsequent introduction of other glyphosate-resistant crops, where available, they have taken a commanding share of the herbicide-resistant crop market, especially in soybean, cotton and canola. The high level of adoption of glyphosate-resistant crops by North American farmers has helped to significantly reduce the value of the remaining herbicide market. This has resulted in reduced investment in herbicide discovery, which may be problematic for addressing future weed-management problems. Introduction of herbicide-resistant crops that can be used with selective herbicides has apparently been hindered by the great success of glyphosate-resistant crops. Evolution of glyphosate-resistant weeds and movement of naturally resistant weed species into glyphosate-resistant crop fields will require increases in the use of other herbicides, but the speed with which these processes compromise the use of glyphosate alone is uncertain. The future of herbicide-resistant crops will be influenced by many factors, including alternative technologies, public opinion and weed resistance. Considering the relatively few recent approvals for field testing new herbicide-resistant crops and recent decisions not to grow glyphosate-resistant sugarbeet and wheat, the introduction and adoption of herbicide-resistant crops during the next 10 years is not likely to be as dramatic as in the past 10 years. 相似文献
14.
Resistance to the acetyl‐coenzyme A carboxylase (ACCase)‐inhibiting herbicides in Lolium rigidum is widespread in grain cropping areas of South Australia. To better understand the occurrence and spread of resistance to these herbicides and how it has changed with time, the carboxyl transferase (CT) domain of the ACCase gene from resistant L. rigidum plants, collected from both random surveys of the mid‐north of Southern Australia over 10 years as well as stratified surveys in individual fields, was sequenced and target site mutations characterised. Amino acid substitutions occurring as a consequence of these target site mutations, at seven positions in the ACCase gene previously correlated with herbicide resistance, were identified in c. 80% of resistant individuals, indicating target site mutation is a common mechanism of resistance in L. rigidum to this herbicide mode of action. Individuals containing multiple amino acid substitutions (two, and in two cases, three substitutions) were also found. Substitutions at position 2041 occurred at the highest frequency in all years of the large area survey, while substitutions at position 2078 were most common in the single farm analysis. This study has shown that target site mutations leading to amino acid substitutions in ACCase of L. rigidum are widespread across South Australia and that these mutations have likely evolved independently in different locations. The results indicate that seed movement, both within and between fields, may contribute to the spread of resistance in a single field. However, over a large area, the independent appearance and selection of target site mutations conferring resistance through herbicide use is the most important factor. 相似文献
15.
Malcolm D. Devine 《Pest management science》1997,51(3):259-264
Resistance to acetyl-coenzyme A carboxylase (ACCase) inhibitors has developed in at least 10 grass weed species in recent years. In most instances, resistance is conferred by an ACCase alteration in the resistant biotypes that reduces sensitivity to aryloxyphenoxypropionate (AOPP) and cyclohexanedione (CHD) herbicides. Analysis of ACCase from many of these resistant weed biotypes suggests the presence of different mutations, each conferring a different pattern and level of resistance to various AOPP and CHD herbicides. In all cases analyzed to date, resistance is controlled by a single dominant or semi-dominant nuclear gene. In several weed biotypes, resistance is conferred by enhanced herbicide detoxification, primarily through elevated expression or activity of cytochrome P450 monooxygenase(s). This mechanism can confer cross-resistance to herbicides from other chemical classes with different modes of action. Finally, multiple herbicide resistance, i.e. the acquisition of several different resistance mechanisms, has been reported in some weed biotypes. ©1997 SCI 相似文献
16.
Repeated use of ACCase‐ and ALS‐inhibiting herbicides in northern Greece has resulted in the evolution of a population of Lolium rigidum resistant to diclofop and chlorsulfuron. The biotype from Athos was highly resistant to diclofop and also exhibited differential cross‐resistance to clodinafop, fluazifop, tralkoxydim and sethoxydim. Assay of ACCase activity confirmed that the resistant biotype was tenfold more resistant to diclofop than the susceptible biotype, suggesting that the resistance mechanism could involve an altered target site. The diclofop‐resistant biotype has also exhibited multiple resistance to chlorsulfuron and the mechanism for this is unknown. Seed‐bioassay was found to be a rapid, cheap and reliable method to identify populations of L rigidum resistant to ACCase inhibitors and chlorsulfuron. Moreover, root elongation in the seed bioassay was more sensitive to ACCase inhibitors and chlorsulfuron than shoot elongation. © 2000 Society of Chemical Industry 相似文献
17.
Powles SB 《Pest management science》2008,64(4):360-365
Glyphosate is the world's most important herbicide, with many uses that deliver effective and sustained control of a wide spectrum of unwanted (weedy) plant species. Until recently there were relatively few reports of weedy plant species evolving resistance to glyphosate. Since 1996, the advent and subsequent high adoption of transgenic glyphosate-resistant crops in the Americas has meant unprecedented and often exclusive use of glyphosate for weed control over very large areas. Consequently, in regions of the USA where transgenic glyphosate-resistant crops dominate, there are now evolved glyphosate-resistant populations of the economically damaging weed species Ambrosia artemissifolia L., Ambrosia trifida L., Amaranthus palmeri S Watson, Amaranthus rudis JD Sauer, Amaranthus tuberculatus (Moq) JD Sauer and various Conyza and Lolium spp. Likewise, in areas of transgenic glyphosate-resistant crops in Argentina and Brazil, there are now evolved glyphosate-resistant populations of Sorghum halepense (L.) Pers and Euphorbia heterophylla L. respectively. As transgenic glyphosate-resistant crops will remain very popular with producers, it is anticipated that glyphosate-resistant biotypes of other prominent weed species will evolve over the next few years. Therefore, evolved glyphosate-resistant weeds are a major risk for the continued success of glyphosate and transgenic glyphosate-resistant crops. However, glyphosate-resistant weeds are not yet a problem in many parts of the world, and lessons can be learnt and actions taken to achieve glyphosate sustainability. A major lesson is that maintenance of diversity in weed management systems is crucial for glyphosate to be sustainable. Glyphosate is essential for present and future world food production, and action to secure its sustainability for future generations is a global imperative. 相似文献
18.
吡啶甲酸类除草剂属合成激素类, 目前登记使用的有效成分包括氨氯吡啶酸、二氯吡啶酸和氯氨吡啶酸, 分别于1963年、1977年和2005年上市, 主要用于阔叶杂草和灌木的茎叶处理防控, 其中氯氨吡啶酸具有土壤封闭活性。吡啶甲酸类除草剂的作用靶标仍未明确, 有可能来自生长素结合蛋白家族。全世界报道的抗吡啶甲酸类除草剂杂草共涉及7种8个生物型。目前, 我国登记的除草剂品种中共有13个复配剂含氨氯吡啶酸和二氯吡啶酸, 1个含氨氯吡啶酸、氯氨吡啶酸和二氯吡啶酸;国外登记的吡啶甲酸类除草剂复配剂主要为与其他激素型除草剂、ALS(乙酰乳酸合酶)抑制剂、ACCase(乙酰辅酶A羧化酶)抑制剂的组合。该类除草剂仍然具有较好的应用前景, 在主要应用场景下常见杂草对氨氯吡啶酸、二氯吡啶酸、氯氨吡啶酸的敏感性仍需系统研究, 该类除草剂主要靶标杂草种群的抗药性水平也亟须检测。 相似文献
19.
This study was conducted to determine a suitable medium for in vitro germination of Alopecurus myosuroides pollen and to develop a reliable test for the rapid screening of ACCase target site‐resistant plants within populations. The assay is based upon germination of pollen in a medium supplemented with ACCase inhibitors. A 0.25% agar medium, containing 200 mg L–1 CaNO3, 100 mg L–1 H3BO3, 200 g L–1 sucrose, was selected as a suitable medium for in vitro pollen germination. At 25 °C, this medium supported a mean germination rate of 85% within two hours. Plants highly resistant (Rh) to aryloxyphenoxypropionate (APP), owing to the expression of an insensitive ACCase, were found to express this resistance in their pollen. In contrast, plants moderately resistant (Rm) to APP herbicides, owing to an enhanced capacity to detoxify herbicides, did not exhibit this resistance in their pollen. Concentrations of 120 μM fenoxaprop and 1000 μM clodinafop were selected as the best for reliable discrimination of the target‐site‐resistant biotypes. At these concentrations there was more than 50% germination of the Rh pollen grains whereas less than 10% of the S and Rm pollen grains germinated. This test, using haploid material, may also permit distinction between homozygous‐ and heterozygous‐resistant individuals. 相似文献
20.
我国水稻田杂草抗药性研究进展 总被引：13，自引：0，他引：13
水稻是我国最主要的粮食作物之一,杂草的危害严重影响了水稻的产量与品质。化学防除仍然是治理水稻田杂草最有效的途径。目前我国水稻田稗属杂草、千金子、马唐、雨久花、野慈姑、异型莎草、耳叶水苋、眼子菜、节节菜、萤蔺等多种杂草对二氯喹啉酸、五氟磺草胺、氰氟草酯、噁唑酰草胺、苄嘧磺隆、吡嘧磺隆、双草醚、噁草酮、乙氧氟草醚等多种常用除草剂产生了抗药性。面对日趋严重的水稻田抗药性杂草的危害,对抗药性杂草进行深入系统的研究以达到科学防治的目的显得尤为重要。本文总结了我国水稻田抗药性杂草的抗药性水平、靶标酶抗药性机理、代谢酶抗药性机理和其他抗药性机理,归纳了抗药性杂草的交互抗性、多抗性发生情况以及抗药性治理现状,分析了我们在杂草抗药性研究与治理方面面临的问题。 相似文献