Cynodon dactylon control with tillage and glyphosate     

A. E. Abdullahi   《Crop Protection》2002,21(10):1093-1100

Field experiments were conducted during two cropping seasons at two sites in Botswana to determine the efficacy of combining glyphosate and tillage in controlling C. dactylon. Treatments consisted of no-tillage, single and double ploughing during winter and spring plus the application of glyphosate at 0, 1.08, and 2.16 kg ha⁻¹ 3–4 weeks after the last ploughing. Visual assessment indicated higher levels of grass control from combining glyphosate and tillage than when these elements were applied separately. However, biomass data indicated that either double ploughing or glyphosate application controlled C. dactylon. A single ploughing followed by glyphosate at 2.16 kg ha⁻¹ after a regrowth of C. dactylon may provide an effective and affordable control method to small-scale farmers.  相似文献   

Possible alternative utilization of Cynara spp.: I. Biomass, grain yield and chemical composition of grain     

S. Foti  G. Mauromicale  S. A. Raccuia  B. Fallico  F. Fanella  E. Maccarone 《Industrial Crops and Products》1999,10(3):3167-228

Globe artichoke (Cynara scolymus L.) and cultivated cardoon (C. cardunculus L. var. altilis DC.) are horticulturally important crop plants. These species have potential as biomass and oilseed crops. We field tested, for 3 years, two artichoke and two cardoon cultivars and one wild cardoon (C. cardunculus L. var. sylvestris Lam.) population on the Sicilian plain of Catania (37°27′ N, 15°04′ E, 10 m a.s.l.). On a 3-year average, the dry aboveground biomass resulted about 31 t ha⁻¹ in both cultivated cardoons, 18.8 t ha⁻¹ in wild cardoon, 13.7 t ha⁻¹ in globe artichoke ‘3/10 V.S.’ and 9.9 t ha⁻¹ in globe artichoke ‘374’ F₁. The caloric values of aboveground biomass (except for seeds), which was not significantly different among genotypes, ranged between 16 005 and 17 028 KJ kg⁻¹ of dry matter. The cultivated cardoon ‘Gigante di Lucca’ had the greatest grain yield (on 3-year average, 2.6 t ha⁻¹), whereas the two globe artichokes had the lowest yield (on 3-year average, 0.5 t ha⁻¹). Regardless of genotypes and years, the grains contain 20.1% crude protein, 24.4% oil, 18.5% crude fiber and 4.1% ash (dry weight basis). The grains of globe artichokes showed the highest crude protein content (21.6%), whereas those of cardoons the highest oil content (25.2%).  相似文献   

Comparing weed and crop seedling response to pre-emergence pendimethalin application in Corchorus olitorius and Abelmoschus esculentus     

M.A.K. Smith   《Crop Protection》2006,25(12):1221-1226

Field experiments and bioassay tests were carried out to evaluate the relative response of the crops, tossa jute (Corchorus olitorius L.) and okra [Abelmoschus esculentus (L.) Moench], and their associated weeds to pre-emergence application of pendimethalin at 0.33, 0.66, 0.99, 1.32 kg ai ha⁻¹ and a pendimethalin+atrazine tank mixture at 1.32+2.05 kg ai ha⁻¹. Bioassay tests were carried out using herbicide solutions of corresponding concentrations and herbicide-treated soil. Field application of pendimethalin at 0.66 kg ai ha⁻¹ and the herbicide mixture in both crops effectively controlled most seedling weeds including Rottboellia cochinchinensis. Euphorbia heterophylla and Calopogonium mucunoides, which persisted from 2 weeks after treatment (WAT) were not controlled. Herbicide application during crop establishment markedly inhibited the growth of both seedling weeds and crops. The mixture caused the highest weed and crop injury. Pendimethalin at 0.33 kg ai ha⁻¹ had minimal effect on these crops. Weed growth, weed tolerance of herbicide treatment and crop seedling injury were higher in tossa jute than that in okra, under the conditions of this study. The use of low pendimethalin doses in an integrated weed management system will ensure effective control of seedling weeds, and prevent crop injury and residue accumulation in edible plant produce.  相似文献   

Application of an emulsifiable mixture of 1,3-dichloropropene and chloropicrin against root knot nematodes and soilborne fungi for greenhouse tomatoes in Italy   总被引：6，自引：0，他引：6  

A. Minuto  M.L. Gullino  F. Lamberti  T. D&#x;Addabbo  E. Tescari  H. Ajwa  A. Garibaldi 《Crop Protection》2006,25(12):1244-1252

A mixture of 1,3-dicloropropene 60.5% w/w and chloropicrin 33.3% w/w (Telone C35 EC) may be registered in Italy for soil drip fumigation. Five experiments on greenhouse tomatoes in Northern, Central and Southern Italy compared the effectiveness of this mixture in comparison with methyl bromide to find the optimum application rate in soils infested by Fusarium oxysporum f.sp. lycopersici, F. oxysporum f.sp. radicis lycopersici, Sclerotium rolfsii, Meloidogyne javanica and M. incognita. Its efficacy against F. oxysporum f.sp. radicis lycopersici and M. incognita was confirmed when applied to soils at 100, 200, 300 and 400 l ha⁻¹ (132.4, 268.4, 402.6 and 536.8 kg ha⁻¹) under gas-tight films with 15–45 mm of application water (900–1200 mg Telone C35 EC l⁻¹). In sandy soils, with slight F. radicis lycopersici infections and with heavy nematode (M. incognita) attacks, the mixture, drip applied at 900 mg l⁻¹ during late summer (fumigation: late summer; transplant: late-summer/autumn; last harvest: early spring), performed well up to 132.4 kg ha⁻¹ (100 l ha⁻¹). In sandy loam soils with slight F. radicis lycopersici infections and severe infections of F. lycopersici and galling nematodes (M. javanica), 268.4 kg ha⁻¹ (200 l ha⁻¹) of the mixture applied at 900 mg l⁻¹ as a drip provided yields similar to those of methyl bromide treated plots both in spring and summer cycles. In sandy loam soils, the diseases (F. lycopersici, F. radicis lycopersici) were controlled at rates 268.4 kg ha⁻¹ (containing 90 kg ha⁻¹ of chloropicrin), but the mixture was ineffective against Sclerotium rolfsii occasionally observed in sandy loam soils. In both sandy and sandy loam soils, no significant relationships were found between the rates of mixture applied (132.4, 268.4, 402.6 and 536.8 kg ha⁻¹) and the degree of nematode infestation.  相似文献   

Glasshouse and field evaluation of fungicides for the control of Septoria apiicola on celery     

T. J. Wicks 《Crop Protection》1990,9(6):433-438

Propiconazole was more effective than either triadimenol or hexaconazole when applied after infection, for the control of Septoria apiicola on celery seedlings. In three out of four glasshouse experiments, 25 mg propiconazole I⁻¹ controlled S. apiicola completely when applied within 3 days after inoculation. Propiconazole and triadimenol at 5–15 mg l⁻¹ were also effective when applied 2 days after inoculation. Soil applications of a granular formulation of triadimenol (up to 20 mg per plant) controlled S. apiicola for 5 weeks on celery seedlings grown in pots and, in the field, granules at 100 mg per plant applied at planting were effective for 6 weeks. In two other field experiments the most effective treatments were foliar sprays of 25 mg propiconazole l⁻¹ applied every 7–10 days with or without the addition of anilazine or chlorothalonil.  相似文献   

Occurrence of resistant strains of Botrytis cinerea to anilinopyrimidine fungicides in table grapes in Chile   总被引：1，自引：0，他引：1  

B. A. Latorre  I. Spadaro  M. E. Rioja 《Crop Protection》2002,21(10):957-961

Effort to control gray mold caused by Botrytis cinerea failed in a table grapevine (Vitis vinifera) vineyard near Santiago, Chile where cyprodinil (Vangard 50 WP), a new fungicide of the anilinopyrimidine group, had been applied alone up to four times per year during two growing seasons. A relatively high frequency (38.5%) of resistant isolates of B. cinerea (EC₅₀ for mycelial growth inhibition varied from 2.9 to 4.84 μg ml⁻¹) may explain the partial loss of field control efficacy obtained. Resistance was correlated with a complete loss of in vivo sensitivity to cyprodinil. Resistant isolates of B. cinerea showed cross resistance to the anilinopyrimidines fungicides mepanipyrim and pyrimethanil. Cyprodinil partially impaired conidia germination and differentially affected conidial germination of resistant and sensitive isolates. Significant differences (p<0.05) in growth rate, sclerotia production and osmotic sensitivity were found among isolates of B. cinerea, but no correlation could be drawn between these biological differences and resistance or sensitivity. This indicates a disruptive selection characteristic of monogenic resistance. Thus, strategies were implemented to avoid the further development and spread of resistance in B. cinerea to the anilinopyrimidine fungicides. To our knowledge this constitutes the first mention of resistance in B. cinerea populations to anilinopyrimidine fungicides in South America.  相似文献   

土壤因子与小粒咖啡品质产量形成关系研究   总被引：1，自引：0，他引：1  

赵明珠  郭铁英  马关润  萧自位  白学慧  周华  苏琳琳 《热带作物学报》2020,41(6):1065-1075

土壤因子是影响小粒咖啡品质产量的主要因子。本研究通过对土壤因子进行相关性分析、改进的灰色关联分析、通径分析及多元线性回归分析,明确影响小粒咖啡品质和产量的主要因子,以指导科学施肥。结果表明:小粒咖啡咖啡因含量的土壤决定因子为海拔（-0.296^*）,总糖含量的土壤决定因子为有机质（0.607^**）和速效磷（-0.232^*）,蛋白质的土壤决定因子为pH（-0.387^**）和有机质（0.227^*）,灰分含量的土壤决定因子为速效磷（0.334^**）,水溶性浸出物含量的土壤决定因子为海拔（-0.494^**）,脂肪含量的土壤决定因子为碱解氮（-0.461^**）,产量的土壤决定因子为速效钾（0.339^**）和有机质（0.248^*）。研究结果可为小粒咖啡优质高产的土壤条件筛选和肥料的减施增效提供一定的理论基础,以期增强优质咖啡种质的适宜性和目的性。  相似文献   

Plant density and self-thinning affect yield and quality of fibre hemp (Cannabis sativa L.)     

H. M. G. van der Werf  M. Wijlhuizen  J. A. A. de Schutter 《Field Crops Research》1995,40(3):153-164

To ascertain the reasons for the high plant mortality in fibre-hemp (Cannabis sativa L.) crops in the Netherlands changes in biomass yield, plant mortality and plant morphology were investigated in a hemp cultivar grown at initial densities of 10, 30, 90 and 270 plants m⁻². At 90 plants m⁻² this cultivar was compared with a high bast-fibre cultivar and a late-flowering cultivar. Rate of canopy establishment and early growth rate increased with increasing plant density. At 90 and 270 plants m⁻², plants died as a result of self-thinning. Self-thinning was associated with a reduced crop growth rate. In self-thinning stands, dry biomass (B, g m⁻²) was related to density of surviving plants (D, m⁻²) as log B = 3.81 − 0.304 log D. At the same crop growth rate, the rate at which plants died from self-thinning was higher in hemp than in other herbaceous dicots. The proportion of stem in the total dry matter increased with increasing plant density. Stem yield was maximum at 90 plants m⁻². Stem quality improved with increasing density as the bark content in the stem increased, but self-thinning reduced the bark content in the stem. Optimum plant density was close to that resulting after self-thinning. In hemp the relationship between yield and optimum plant density is approximated by the equation of its self-thinning line. The late-flowering cultivar yielded more than the other two cultivars because it grew faster during the latter part of the growing season.  相似文献   

On-farm measurements of nitrogen fixation by winter and summer legumes in the Hill and Terai regions of Nepal     

S. L. Maskey  S. Bhattarai  M. B. Peoples  D. F. Herridge 《Field Crops Research》2001,70(3):540-221

Results are reported from on-farm surveys of N₂ fixation in Nepal, conducted between 1994 and 1999, involving the summer legumes soybean, mashbean and groundnut, and the winter legumes lentil and chickpea, at various locations in the Hill and Terai regions of Nepal. Additional less-detailed data were also collected for pigeonpea, grasspea and fababean. There were a total of 107 crops in the major and minor surveys. Estimates of the proportion of legume nitrogen (N) derived from N₂ fixation (Pfix) were determined using the natural ¹⁵N abundance method. Mean estimates of Pfix for the various species were 62% (soybean), 47% (mashbean), 57% (groundnut), 78% (lentil), 79% (chickpea), 75% (pigeonpea), 87% (grasspea) and 85% (fababean). Estimates of total N fixed (including roots) were 59 kg N ha⁻¹ (soybean), 28 kg N ha⁻¹ (mashbean), 153 kg N ha⁻¹ (groundnut), 72 kg N ha⁻¹ (lentil), 84 kg N ha⁻¹ (chickpea), 412 N ha⁻¹ (pigeonpea) and 80 N ha⁻¹ (grasspea and fababean). The on-farm measurements of N₂ fixation were generally similar to those of experimental crops in the same areas of the country. Correlation matrices of soil fertility parameters, shoot dry matter and N, and N₂ fixation revealed that the total amounts of N fixed were influenced primarily by crop growth. Based on the above figures, it was estimated that approximately 30,000 t N were fixed annually in Nepal by legumes, valued at US$ 15 million.  相似文献   

Fungicidal control of metalaxyl-insensitive strains of Bremia lactucae on lettuce     

T.G. Wicks  B. Hall  P. Pezzaniti 《Crop Protection》1994,13(8):617-623

Strains of B. lactucae insensitive to 0.01 g a.i. l⁻¹ metalaxyl have been detected in all the lettuce-growing districts of South Australia. Despite this, applications of a formulated mixture of 0.25 g a.i. l⁻¹ metalaxyl and 2 g a.i. l⁻¹ mancozeb every 7–10 days controlled downy mildew in field experiments, where similar applications of either metalaxyl or mancozeb alone were ineffective. Protectant schedules of either 0.3 g a.i. l⁻¹ dimethomorph or 2–2.4 g a.i. l⁻¹ phosphonic acid also controlled downy mildew, but were less effective than the metalaxyl/mancozeb formulation. Mixtures of either dimethomorph or phosphonic acid with mancozeb enabled the time between the sprays to be increased to 14 days without significantly reducing control. Fungicide applications increased marketable yield by 60% compared with that of the unsprayed plants. Drenching seedlings with 4 g a.i. l⁻¹ phosphonic acid controlled downy mildew for at least 14 days and should prevent the spread of the disease from plant production areas.  相似文献   

Improving spring and summer fungicide treatments for winter barley     

P. Gladders  M.J. Hims 《Crop Protection》1994,13(8):597-606

The response of winter barley to broad-spectrum fungicides applied at first node stage [growth stage (GS) 31] or as two-spray programmes [first node stage and flag leaf to ear emergence (GS 39–59)] was examined at 22 sites over five years during 1986–1990. Single sprays were also applied at an intermediate timing [third node to flag leaf emerging (GS 33–37)] at all sites in 1989 and 1990. The mean response to the single spray at GS 30–31 was 0.86 t ha⁻¹ but ranged from 0.25 to 3.03 t ha⁻¹ (4–80%). With the addition of a late spray (GS 39–59) of propiconazole plus tridemorph to the spring treatment, mean response was 1.25 t ha⁻¹ [range 0.35–3.32 t ha⁻¹ (5–87%)]. There were some differences between fungicides and fungicide mixtures applied at GS 31 and a prochloraz component was needed for effective control of eyespot. Seasonal differences in the mean response to the GS 39–59 treatment reflected differences in disease pressure after GS 59. The GS 30–31 treatment failed to maintain disease control up to the milky ripe stage (GS 75) in years when disease pressure was high. Yield responses were correlated with disease severity on leaf 2 at the time of the spring (GS 31) application and with the degree of control of eyespot on the stem base and of foliar diseases on the flag leaf at GS 75. A single spray applied at GS 33–37 gave comparable yields to the two-spray programmes and has potential to improve profitability where disease levels are low at the first node stage.  相似文献   

Rice yield and productivity gaps in irrigated systems of the forest zone of Côte d'Ivoire     

Mathias Becker  David E. Johnson 《Field Crops Research》1999,60(3):367-208

Much of the rapidly growing demand for rice in West Africa will be met from increased production in irrigated lowlands, which cover about 12% of the regional rice-growing area. A large potential for expansion of irrigated areas exists particularly in the inland valleys of the humid forest zone. Current production is characterized by large variability in productivity, management practices and production constraints. Quantifying the variability in rice yield and identifying the determining factors are prerequisites to the development of site-specific recommendations and to improved targeting of technologies. Diagnostic on-farm trials were conducted on 64 irrigated lowland fields in the humid forest zone of southern Côte d'Ivoire, in 1995–1996. This was a part of the regional gradient study of irrigated systems from the desert margin to the humid forest zone. Cropping calendars, field operations and input use were monitored. Weed biomass, rice N uptake, and grain yield were determined in farmers' fields as well as in super-imposed, researcher-managed subplots (clean weeding, no N control, and mineral fertilizer N application). Rice yield potential was simulated by using the Oryza-S crop growth model. Yield losses were attributed to management factors based on performance of rice in researcher-managed subplots (management-related yield gap) and by multiple regression with management options. Grain yields varied between 0.2 and 7.3 Mg ha⁻¹ with mean yields of 3.2 in partially and 4.2 Mg ha⁻¹ in fully irrigated systems, 44% and 57% of the potential yield of 7.3 Mg ha⁻¹, respectively. Age of seedlings at transplanting, timeliness of operations and application of P fertilizer were correlated to yield and explained 60% of the observed variability. Grain yield was correlated with N uptake (r² = 0.93^***) but not with N application rate. Split application of mineral fertilizer N was associated with a 0.48 Mg ha⁻¹ yield increase (p = 0.002), regardless of the quantity applied. Additional weeding increased yield only in systems with imperfect irrigation. Weed biomass was reduced with improved water control and it increased with age of seedlings at transplanting, and was higher in direct-seeded than in transplanted rice. Echinochloa spp. were the most common weeds in fully irrigated systems and Panicum laxum was more common in the imperfectly irrigated fields. While improved water management was associated with substantial rice yield increases (1.16 Mg ha⁻¹), the timeliness of transplanting, weeding and N fertilization appears to be the key to increased rice yields in the forest zone of West Africa.  相似文献   

水稻二化螟病原线虫N-Yz1的分离鉴定及其感染特性     

刘琴  夏杨  韩光杰  李传明  陆玉荣  黄立鑫  祁建杭  徐健 《中国水稻科学》2022,36(6):639-646

【目的】二化螟是危害水稻的重要害虫，其钻蛀为害，防治困难，严重威胁水稻生产。本研究对感染二化螟昆虫病原线虫的分离筛选和感染特性进行研究，期望为水稻钻蛀害虫危害的防治提供新的途径和方法。【方法】自越冬代二化螟幼虫体内通过组织分离筛选，获得野外感染二化螟的昆虫病原线虫，室内接种实验分析分离昆虫病原线虫对二化螟的感染活性和作用特性。【结果】自然罹死二化螟幼虫体内分离获得野外感染的昆虫病原线虫N-Yz1，接种4龄二化螟幼虫导致感染并死亡，后期虫体内产生大量形态一致的侵染期线虫(IJ)。形态学观察结合rDNA-ITS序列分析，鉴定分离昆虫病原线虫隶属于斯氏线虫属，与已鉴定种小卷蛾斯氏线虫同源性达99.87%。昆虫病原线虫N-Yz1对不同龄期二化螟幼虫都有较高感染致死作用，40头(IJ)的线虫接种剂量，对低龄(2~3龄)二化螟致死速度快、致死率高， 接种2 4 h 就出现感染致死个体，48 h后幼虫全部死亡。高龄幼虫(4~6龄)感染致死速度较慢，死亡高峰发生在接种线虫后的48 h。N-Yz1接种剂量与二化螟死亡率呈显著正相关，剂量超过30 IJs/头时，4龄二化螟感染死亡率达90%以上。N-Yz1对二化螟幼虫具有明显搜索能力，在距离寄主害虫0-30 cm的范围内，通过迁移扩散，感染二化螟并导致其死亡。【结论】分离昆虫病原线虫N-Yz1具有较强的感染致死能力，表现出防治水稻钻蛀害虫二化螟的潜在应用价值。  相似文献   

Effect of antagonistic Fusarium spp. and of different commercial biofungicide formulations on Fusarium wilt of basil (Ocimum basilicum L.)     

Andrea Minuto  Giovanni Minuto  Quirico Migheli  Massimo Mocioni  M.Lodovica Gullino 《Crop Protection》1997,16(8):765-769

The ability of antagonistic Fusarium spp. to control Fusarium wilt of basil caused by F. oxysporum f. sp. basilici was tested under glasshouse conditions from 1994 to 1996. Fusarium oxysporum antagonistic strain 251/2, a protoplast fusion-derived hybrid coded FI-11 and F. moniliforme strains TF4 and TF4 RB were able to decrease (P = 0.05) the incidence of Fusarium wilt significantly. Biocontrol was consistent, especially when the antagonists were applied by mixing a talc preparation of propagules in the substrate (10⁵ CFU/ml of substrate) 1–2 weeks before sowing or at sowing. Seed coating with the biocontrol strains (10⁸ CFU/g of seed) did not generally provide sufficient disease containment, whereas the combination of substrate treatment and seed coating did not improve wilt control in comparison with substrate treatment alone. Two commercial formulations, based on antagonistic F. oxysporum strains and one containing the antagonistic strain K61 of Streptomyces griseoviridis, were generally not sufficiently effective at the tested dosages.  相似文献   

Wheat stem sawfly (Cephus pygmaeus L.) damage; impacts on grain yield, quality and marketing prices in Anatolia     

&#x;rfan zberk  Ayhan Atl&#x;  Abuzer Yücel  Fethiye zberk  Yalc&#x;n Cokun 《Crop Protection》2005,24(12):1054-1060

This study aimed to draw the attention of the all stake holders attention to an underestimated insect pest of wheat in Southeastern Anatolia. The field studies were carried out in the experimental field of GAP Training, Extension and Research Center in Koruklu in 2003–2004 cropping season.

It was found that the number of sawfly damaged spikes varied between 6 and 12% in durum wheat and 8 and 12% in bread wheat. Comparing healthy grains, grain weight spike⁻¹ decreased significantly, giving 0.430 g less kernel weight in durum wheat and 0.385 g in bread wheat. Some of the grain quality characteristics of both sawfly damaged and healthy spikes were tested and it was found that protein content (%) in durum wheat, and 1000 kernel weight in bread wheat were reduced significantly, whilst, the SDS sedimentation value in bread wheat increased significantly for sawfly damaged grains. Grain yield losses by sawfly infestation were found to be 2.23% in durum wheat and 3.32% in bread wheat. Marketing price studies showed that sawfly damage reduced it significantly, resulting in $ 0.016 kg⁻¹ less price in bread wheat. But this was not serious for durum wheat.

It was concluded that income loss, depending on grain yield loss, un-harvestable broken spikes and lower marketing price of sawfly damaged grains, could be no less than $ 68.8 ha⁻¹ in durum wheat and $ 68.6 ha⁻¹ for bread wheat. Therefore, some control methods are required for sawfly infestation, where damage is already over the economic threshold (10–15% stem cut by pest) especially in bread wheat.  相似文献   

Increased frequency of phytosanitary pod removal in cacao (Theobroma cacao) increases yield economically in eastern Peru     

W. Soberanis  R. Ríos  E. Arvalo  L. Zúiga  O. Cabezas  U. Krauss 《Crop Protection》1999,18(10):109-685

Diseases caused by Moniliophthora roreri (moniliasis), Crinipellis perniciosa (witches’ broom) and Phytophthora palmivora (black pod) are the most important factors limiting cocoa production in Peru and cultural management is considered to be the only practical means of control for the smallholder. The objective of this study was to develop an epidemiologically and economically sound control recommendation based on the frequency of phytosanitary pod removal. Weekly removal of diseased pods reduced the incidence of diseases significantly in comparison with fortnightly removal. In three fields, moniliasis was decreased by 26–41%, black pod by 35–66% and witches’ broom on pods by 14–57%. The cumulative effect was a consistent yield increase from an average of 504–660 kg ha⁻¹ yr⁻¹. Returns compensated for increased labour costs. Weekly pod removal was 32% more profitable. Time-course analyses indicated that weekly removal should be practised throughout the year under field conditions of eastern Peru.  相似文献   

球孢白僵菌Bbchitinase 1和Bbchitinase 2在侵染宿主过程中的不同作用     

汪永松  耿涛  卢芙萍  武华周  娄德钊  涂娜娜  王树昌 《热带作物学报》2021,42(4):1092-1098

球孢白僵菌（Beauveria bassiana）在宿主昆虫体壁穿透和体内定植过程中分泌了Bbchitinase 1和Bbchitinase 2两种胞外几丁质酶,但二者的作用分工尚不明确。本文通过菌株胞外总几丁质酶活性与侵染毒力的相关性分析,Bbchitinase 1和Bbchitinase 2基因表达水平与侵染毒力的相关性分析,Bbchitinase 1和Bbchitinase 2的生物信息学分析以及在侵染过程中的表达模式分析,以期明晰Bbchitinase 1和Bbchitinase 2在菌株侵染宿主过程中的作用分工。结果表明,菌株胞外几丁质酶活性与侵染毒力呈正相关,但Bbchitinase 1和Bbchitinase 2表达水平不同。菌株间Bbchitinase 1表达水平与菌株侵染毒力（MMT和LT₅₀）呈显著正相关,而Bbchitinase 2表达水平却与侵染毒力不相关。生物信息学分析结果显示,Bbchitinase 1和Bbchitinase 2具有显著的结构和功能差异,可能结合不同的配体蛋白,参与不同的代谢过程。表达模式分析结果表明,Bbchitinase 1和Bbchitinase 2基因在侵染宿主过程中均上调表达;但Bbchitinase 1仅在侵染家蚕的体壁穿透阶段显著上调表达,而Bbchitinase 2在芽生孢子生成阶段和菌丝大量增殖阶段显著上调表达。以上研究结果说明,Bbchitinase 1可能参与菌株侵染早期的侵入钉形成和体壁穿透过程,与侵染毒力呈正相关;而Bbchitinase 2可能参与侵染过程中的芽生孢子发育和菌丝大量增殖代谢调控。本研究明晰了球孢白僵菌不同几丁质酶在侵染过程中的作用分工,建立了以Bbchitinase 1基因表达水平判定菌株毒力的数学模型,为高毒、高稳定性球孢白僵菌生防菌株的筛选提供重要科学依据,对提升绿色生态防治应用效果具有重要意义。  相似文献   

Concentrations in soil and efficacy of drip-applied 1,3-D+chloropicrin and metam sodium in plastic-mulched sandy soil beds     

B.L. Candole  A.S. Csinos  D. Wang 《Crop Protection》2007,26(12):1801-1809

Concentrations in soil and efficacy of 1,3-dichloropropene (1,3-D)+chloropicrin and methyl isothiocyanate (MITC) against the survival of Phytophthora capcisi, Rhizoctonia solani and yellow nutsedge (Cyperus esculentus L.) were studied in plastic-mulched Southern Georgia sandy soil beds. Beds were treated with InLine (60.8% 1,3-D+33.3% chloropicrin) at 93.5, 187, and 280.5 l ha⁻¹ and Vapam (42% metam sodium) 233.8, 467.5 and 701.3 l ha⁻¹. 1,3-D+chloropicrin and MITC concentrations in soil were monitored after drip application for 7 d and 240 h, respectively, from pre-selected sites in beds. 1,3-D+chloropicrin and MITC concentrations decreased with time and distance from the emitter. Survival of P. capsici, R. solani and yellow nutsedge in treated beds was higher with distance from the emitter. These methyl bromide alternatives did not diffuse laterally at effective concentrations beyond the point of application. Improved application techniques are required to improve the lateral distribution of these alternatives.  相似文献   

Effects of irrigation, nitrogen and gypsum on yield, nitrogen accumulation and water use by wheat   总被引：1，自引：0，他引：1  

D. M. Whitfield  C. J. Smith  O. A. Gyles  G. C. Wright 《Field Crops Research》1989,20(4):261-277

Responses of wheat grown on a heavy clay soil in the Goulburn-Murray Irrigation Region of south-eastern Australia to a factorial combination of three irrigation treatments and nitrogen and gypsum application were investigated.Irrigation treatments included a rainfed control (treatment R_F) and irrigation on either a weekly (treatment I_w) or fortnightly (treatmnt I_F) basis beginning in spring and maintained until physiological maturity. Nitrogen was applied at 0 and 150 kg N ha⁻¹ (treatments N₀ and N₁₅₀, respectively) and gypsum at 0 and 5 t ha⁻¹. Nitrogen and gypsum treatments were applied at sowing.

Yield increased from a mean of 4 t ha⁻¹ treatment R_F to 6.6 t ha⁻¹ in treatments I_F and I_W, largely because of promotive effects of irrigation on kernel weight (increase from 31 mg to 42 mg kernel⁻¹ and kernel spikelet⁻¹ (1.4 as compared with 1.7). Seasonal conditions and the relative fertility of the site were sufficient to maximise spike number and spikelet spike⁻¹. Nitrogen increased kernel spikelet⁻¹ but effects on yield were not significant because of a decrease in kernel weight. Effects of gypsum on yield were not significant.

Water-use efficiency of both rainfed and irrigated treatments was ca. 1.25 g grain kg⁻¹ H₂O. However, transpirational water-use efficiency, calculated after allowing 110 mm water for soil evaporation, fell from 2 g kg⁻¹ in treatment R_F to 1.7 and 1.5 g kg⁻¹ in treatments I_F and I_W, respectively. The decrease was ascribed, in part, to lodging and soil evaporative losses may have been in excess of 110 mm with more frequent irrigation. Effects of N on water use could not be distinguished, again because of the initial fertility of the site, which supported rapid growth and resulted in complete canopy closure.

Nitrogen and irrigation treatments had independent effects on the concentration of N in the grain (%N_G) which increased by a mean of 0.6% with N treatment despite a decrease in N harvest index (HI_N) from 0.77 to .70. Irrigation decreased %N_G by approximately 0.5%. Approximately 90 kg pN ha⁻¹ was found in the grain of treatments R_FN₀, I_WN₀, I_wN₀ and R_FN₁₅₀ and differences in %N_G in these treatments attributed to a ‘dilution’ effect mediated by the increase in yield effected by irrigation. The grain accounted for approximately 115 kg N ha⁻¹ in treatments I_FN₁₅₀ and I_WN₁₅₀, countering the inverse relationship between %N_g and yield despite the increase in HI_N index caused by N application.  相似文献   

Growth, gas exchange, water relations, and ion composition of Phaseolus species grown under saline conditions     

Jeannette S. Bayuelo-Jimnez  Daniel G. Debouck  Jonathan P. Lynch 《Field Crops Research》2003,80(3):207-222

We examined the effects of salinity on four wild (Phaseolus angustissimus, Phaseolus filiformis, P. microcarpus, and P. vulgaris) and two cultivated (P. acutifolius and P. vulgaris L.) Phaseolus species. Relative growth rate (RGR, g g⁻¹ per day), unit leaf rate (ULR, g m⁻² per day), leaf area ratio (LAR, m² g⁻¹), specific leaf area (SLA, m² g⁻¹), leaf weight ratio (LWR, g leaf g⁻¹), and rate of ion uptake were calculated for the period between 10 and 20 days after planting. Salinity significantly reduced RGR, ULR, LAR, and SLA whereas LWR showed no definite trend. In all species, except in P. filiformis, ULR, but not LAR, was significantly correlated with RGR, indicating that ULR was an important factor underlying the salinity-induced differences in RGR among species. In P. filiformis, high salinity reduced SLA, and consequently LAR. The significant correlation of SLA and LAR with RGR suggested that growth components affecting leaf area expansion were the primary factors explaining the inhibition of growth in this species. Increasing salinity progressively decreased leaf water vapor conductance. The rate of CO₂ assimilation decreased gradually with salinity, showing significant reductions only at the highest salt level (80 mM NaCl). Approximately two-thirds of the reduction in CO₂ assimilation rate at high salinity was attributable to reduced stomatal conductance. In P. filiformis, however, neither stomatal conductance nor CO₂ assimilation were affected by salt stress. Leaf water and osmotic potentials declined significantly as stress intensified. However, osmotic adjustment permitted the maintenance of positive turgor throughout the growth period. Salinity had a significant effect on tissue concentrations of Na⁺, K⁺, Ca²⁺, and Cl⁻ and on the uptake rate of Na⁺, K⁺, Ca²⁺, and Cl⁻. Thus, in addition to the toxic effects of high concentrations of Na⁺ and Cl⁻ in plant tissue, saline-induced changes in mineral nutrient uptake likely contributed to the reduction of plant growth. It appears that salt tolerance in P. filiformis is associated with Na⁺ exclusion and organ Na⁺ compartmentation in roots and stems as well as sustained K⁺ concentration in leaves and better stomatal control through osmotic adjustment. All other Phaseolus species are Na⁺ excluders, and maintained turgor-driven extension growth by accumulating Cl⁻ (osmotic adjustment), but subsequent weight gain reductions suggest that this led to ion toxicity.  相似文献