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1.
张艳  阎俊  陈新民  何中虎 《麦类作物学报》2007,27(5):803-808835
为了明确糯麦配粉对小麦品质的影响,利用中国北部和黄淮冬麦区的三个小麦主栽品种京411、豫麦49和豫麦34,研究了添加10%、15%、20%、25%和30%不同比例的糯小麦面粉后其蛋白质、淀粉特性及面条品质的变化.结果表明,随着添加比例的提高,面粉蛋白质含量和沉淀值有所增加,和面时间和衰落势无显著变化.直链淀粉含量随添加量的增加而逐渐降低,高峰粘度和反弹值则均有所下降.直链淀粉含量与面条的粘弹性、光滑性和总评分呈二次曲线关系,直链淀粉含量为24%~25%时,面条的粘弹性、光滑性和总评分最好,添加15%~20%的糯小麦面粉即可达到这一效果.添加糯小麦面粉对面条色泽和表观状况无显著影响.  相似文献   

2.
Partial waxy (reduced-amylose) and fully waxy (amylose-free) tetraploid durum wheats (Triticum turgidum L. var durum) were developed by introgression of null alleles at the Wx-A1 and Wx-B1 loci from common hexaploid wheat (Triticum aestivum L.). Purified starches were obtained from each genotype, and chemically modified by: 1) cross-linking with phosphorus oxychloride, 2) substitution with propylene oxide, and 3) sequential cross-linking with phosphorus oxychloride followed by substitution with propylene oxide. Functional properties were compared to blends of waxy and wild-type durum starches of known amylose contents. Significant differences in functionality were observed amongst the genotypes and blends after each modification. Waxy (0% amylose) and wild-type (30% amylose) typically were at the extremes of the observed ranges of functional properties. In general, the functional properties of the chemically modified starches were dependent upon amylose content. Starches from Wx-B1 null lines (24% amylose), were an exception. After substitution, such starches had the significantly highest value for RVA final viscosity, and generally performed in a manner similar to starch blends of 12–18% amylose.  相似文献   

3.
Partial waxy (reduced amylose) and fully waxy (amylose-free) tetraploid durum wheats (Triticum turgidum L. var. durum) were used to investigate the relationships between both intra- and inter-granular variation in amylose concentration and starch functionality. Starches isolated from each genotype (intra-granular amylose variants) were compared to those of commercially available hexaploid wild-type and waxy starches, and functionalities compared to blends (inter-granular variants) of durum waxy and wild-type starches of 0, 6, 12, 18, 24, & 30% amylose content. Starch particle size distributions were not significantly different amongst partial waxy and wild-type genotypes; waxy samples had significantly smaller mean starch granule size. Few significant differences for crystallite melting and related intrinsic heat as determined by differential scanning calorimetry (DSC) were observed. The detected differences in starch gel color or gel strength generally were observed for the waxy samples relative to wild-type. Numerous significant differences were observed via Rapid-Visco Analysis (RVA). Pasting peak viscosity and breakdown were inversely proportional to % amylose. Wx-B1 null final viscosity differed significantly from that of all other blends and genotypes, demonstrating that genotypic differences exist amongst the partial waxy types, independent of amylose concentration per se.  相似文献   

4.
小麦淀粉与面条质量关系的研究进展   总被引:5,自引:0,他引:5  
小麦淀粉品质对白盐面条的质量(尤其是煮后的感官特性)有重要影响。直链与支链淀粉的含量及比例是影响面条质量的重要因素,是造成不同小麦品种淀粉糊化和膨胀特性及面条质量差异的物质基础。较低直链淀粉含量的小麦粉具有较好的糊化和膨胀特性,制作的面条煮制时吸水率高,烹调损失低,具有较高的感官评分。优质白盐面条的直链淀粉含量应在22%左右。峰值黏度、稀懈值、峰值时间是影响面条质量的重要糊化参数,这3项参数高的小麦粉适合制作优质面条。高膨胀势或膨胀体积的小麦粉制作的面条中等偏软,光滑且富有弹性,可以作为面条用小麦的重要选择标准。一般认为,直链淀粉含量较低、峰值黏度和稀懈值高、峰值时间长、膨胀势或膨胀体积高的小麦粉适合制作优质白盐面条。其中,直链淀粉含量、峰值黏度和膨胀势是优质面条小麦评价的关键品质性状。  相似文献   

5.
Quality characteristics of northern-style Chinese steamed bread (CSB) prepared from two soft red winter (SRW) wheat flours blended with 0–30% waxy wheat flour (WWF) were analyzed to estimate the influence of starch amylose content. The increased proportion of WWF in blends raised mixograph absorption with insignificant changes in protein content and dough strength-related parameters. WWF incorporation generally increased specific volume and crumb softness of CSB. The analysis of covariance revealed that CSB quality attributes were little affected by protein content and dough strength-related parameters, indicating that starch amylose content was largely responsible for the changes in CSB quality. Flour blends with 5–10% WWF, of which starch amylose content was 22.4–24.7%, produced CSB with superior crumb structure compared to other blends, but insignificant changes in surface smoothness, stress relaxation and total score compared to the respective control wheat flours. Flour blends with 15% WWF to produce a starch amylose content of 21.4–22.7% exhibited reduced staling of CSB with total scores comparable to the respective control wheat flours. CSB prepared from blends with more than 10% WWF exhibited a higher soluble starch content, indicative of reduced starch retrogradation, than that prepared from wheat flours without WWF during storage for 3 days.  相似文献   

6.
The rheological properties of wheat flour under processing such as extrusion (with 28% moisture content, wet basis) are influenced by the molecular changes its components undergo during processing. But, there was no simple relationship between the wheat-flour characteristics and their rheological properties. In order to investigate the quantitative and qualitative effects of the individual flour components on rheological properties, model blends of wheat starch and wheat gluten with different starch/gluten ratios were studied. The effects of gluten and starch quality were also investigated by using different gluten types and by modifying the amylose content of starch, respectively. The shear viscosity of the blends, determined by capillary rheometry under controlled conditions (35% moisture content, 140 °C), was observed to be modified by both gluten and amylose content. The changes undergone by wheat gluten under these conditions were analysed by HPLC, to determine the levels of unextractable polymeric proteins, and by Lab-on-a-Chip analysis of protein composition, to follow the polymerisation of protein under processing. This study indicated that in low hydrated products in the molten state, shear viscosity is affected by the structure of the blends as determined by fluorescence microscopy and by the molecular changes occurring during processing.  相似文献   

7.
一些小麦品种的淀粉特性分析   总被引:4,自引:0,他引:4       下载免费PDF全文
为了筛选出淀粉品质优良的面条小麦品种,分析了200份国内小麦品种(系)和6份澳大利亚小麦品种的淀粉特性及Waxy蛋白亚基组成.与国外优质面条小麦相比,国内小麦的淀粉品质总体上有一定差距,但主要淀粉特性的变异范围比较广,如直链淀粉含量变幅为17.79%~27.14%,膨胀势为3.77~10.45g/g,峰值粘度为491.50~4 497.00 cP,低谷粘度为74.00~3 042.00 cP,崩解值为584.50~1 986.50 cP.国内小麦品种(系)中有15份缺失Wx-B1蛋白亚基,其直链淀粉含量、峰值粘度和崩解值的平均值优于正常类型品种(系),如花培18-5直链淀粉含量低于19%,峰值粘度高于4000 cP,崩解值高于1 500 cP.河南省小麦品种(系)的淀粉特性优于其他省市.  相似文献   

8.
小麦籽粒淀粉合成动态及糊化特性的基因型差异   总被引:5,自引:1,他引:5       下载免费PDF全文
为了明确不同基因型小麦品种籽粒灌浆过程中的淀粉合成动态,为小麦的高产优质栽培提供参考信息,对6个不同基因型小麦籽粒形成过程中淀粉组分的变化及淀粉糊化特性进行了分析。结果表明,非糯性小麦的直链淀粉含量随着花后天数的增加呈上升的趋势,糯性小麦则呈先上升后下降的趋势,所有品种的支链淀粉含量均呈上升的趋势;不同基因型小麦直链、支链淀粉积累速率均呈单峰曲线图,不同品种直链淀粉最大积累速率出现的时间不同,而支链淀粉最大积累速率出现的时间均在花后21d;不同小麦品种的峰值粘度、低谷粘度、最终粘度均随着花后天数的增加而上升;不同品种淀粉糊化特性不同:糯性小麦之间在糊化时间、反弹值上无差异,而在峰值粘度、低谷粘度、最终粘度、稀懈值上的差异达显著水平;对于非糯性小麦,豫麦50和其他品种的多数糊化参数差异达显著水平;糯小麦和非糯小麦在糊化参数上的差异达1%显著水平。  相似文献   

9.
Dough rheological properties and noodle-making performance of non-waxy whole-wheat flour (WWF) with partial- or full-waxy (PW- or FW-) WWF substitution were studied. The substitution levels were 0, 250, 500, 750, and 1000 g/kg, respectively. FW-WWF reduced the peak viscosity and pasting temperature of WWF blends as its substitution level was increased due to its higher proportions of B-type starch granules and short amylopectin chains, while PW-WWF increased peak viscosity with the increasing substitution level because of its higher amylopectin content. As demonstrated by farinograph and rheometer measurements, FW-WWF interfered with gluten development because of the increased competition for water by arabinoxylans and amylopectin; however, PW-WWF enhanced dough strength due primarily to its increased protein content. Consequently, FW-WWF showed a detrimental effect on cooked noodle texture as the cooked noodle hardness was reduced by 50% at the 1000 g/kg substitution level. In contrast, PW-WWF enhanced noodle integrity and elasticity by increasing cooked noodle cohesiveness and resilience by 10.1% and 14.8%, respectively, at the 1000 g/kg substitution level. The results suggest that with waxy WWF substitution, the changes in starch composition, arabinoxylans, and protein content could modify the interactions among flour components and influence the quality characteristics of noodle products.  相似文献   

10.
为给面条小麦亲本材料的筛选提供可靠依据,用SDS-PAGE和STS-PCR结合的方法鉴定了24个品种的Wx蛋白亚基组成,测定了这些品种的淀粉含量、膨胀势和RVA参数。结果表明,与正常材料相比,Wx蛋白亚基缺失材料的胚乳中直链淀粉含量减少,支链淀粉和总淀粉含量变化不明显,Wx蛋白亚基缺失的数目越多,直链淀粉含量减少得就越多,糯麦的直链淀粉含量最少。在本研究参试材料特定遗传背景干扰的情况下,在Wx蛋白的三个亚基中Wx-A1蛋白亚基对直链淀粉含量影响最大。Wx蛋白亚基的缺失还使面粉的膨胀势、RVA参数的高峰粘度、崩解值增大,峰值时间延长,而使最终粘度、回生值和糊化温度降低,Wx蛋白亚基缺失的数目越多,这些特性变化的幅度越大。在缺失单个亚基的情况下,Wx-B1亚基的缺失使小麦粉的淀粉特性表现最好。相关分析表明,直链淀粉合成数量的减少是导致上述淀粉特性发生变化的主要原因。  相似文献   

11.
为探明氮肥对小麦籽粒淀粉组分和理化特性的影响,采用田间试验,分析了4个施氮量(0、100、200、300kg·hm~(-2))对糯小麦(农大糯50222)和非糯小麦(轮选987)籽粒淀粉组分与理化特性的影响。结果表明,随施氮量的增加,小麦籽粒淀粉含量降低,轮选987籽粒中B型淀粉粒的数目占比增加,而农大糯50222籽粒的B型淀粉粒数目占比呈减少趋势;增施氮肥能显著提高小麦籽粒淀粉的峰值黏度和谷值黏度。相同施氮量处理下,轮选987淀粉的被测糊化特征参数(除谷值黏度外)均大于农大糯50222。施氮量不同,2个品种籽粒淀粉X-衍射图谱中各峰的位置和相对强度明显不同。随施氮量的增加,轮选987淀粉的相对结晶度增大,而农大糯50222淀粉的相对结晶度减小。相关分析表明,小麦籽粒的直链淀粉含量和直支比与最终黏度、稀澥值、反弹值、糊化温度和峰值时间呈显著正相关,与谷值黏度和相对结晶度呈显著负相关;支链淀粉含量反之。小麦籽粒的B型淀粉粒数目占比与峰值黏度、稀澥值呈显著负相关。综上所述,施氮量可影响小麦籽粒的淀粉含量和粒度分布,进而改变其糊化特性和晶体特征。  相似文献   

12.
Granule-bound starch synthase, also known as the waxy protein catalyses the synthesis of amylose in wheat endosperm starch. In durum wheats, the genes encoding GBSS are present at the two Wx loci on chromosome 7A and 4A (a segment of 7B that has been translocated). Several null Wx-B1 (missing GBSS protein from chromosome 4A) durum lines were produced from crosses with null-4A bread wheats backcrossed to durum wheats. Semolina milled from 4 normal and 7 null-4A durum wheat lines grown over two seasons (1999 and 2000) in South Australia were analysed for amylose content, starch pasting properties as measured by the Rapid Viscoanalyzer (RVA), swelling power and starch damage, protein content and electrophoretic protein analysis. Spaghetti was prepared with a micro-scale extruder and the cooked pasta evaluated for cooking loss, firmness, stickiness and water absorption. The null-4A lines had significantly lower (ca. 5%) amylose content, higher starch peak viscosities and semolina swelling power. The pasta derived from the null-4A lines had lower cooking loss and in 1999 was more adhesive than the non-waxy lines. Cooking loss was correlated with amylose content, peak starch viscosity, swelling power of semolina and cooked pasta adhesiveness. Semolina swelling power was highly correlated with RVA peak viscosity. Waxy durum wheats appear to have an advantage over the normal types in terms of lower cooking loss, widely used as an indicator of pasta cooking quality.  相似文献   

13.
糯小麦(Waxy wheat)因有高于普通小麦的支链淀粉含量,在食品和工业方面有很大的应用价值和潜力,自发现后就成为各国小麦研究的重点之一.通过多年的努力,人们在糯小麦的遗传机理、育种理论和方法、品质性状及利用等方面的研究都取得了很大的进展,并在1995首次育成了全糯性小麦.糯小麦根据其糯蛋白缺失情况可分为8种类型.控制3个Waxy位点的Wx-A1,Wx-B1和Wx-D1基因分别位于染色体的7AS、4AL和7DS上.3个基因的核苷酸序列长度分别是2 781、2 794、2 862 bp,基因的完全编码区含有11个外显子和10个内含子,在核苷酸序列上三个糯基因彼此之间有惊人的同源性.研究表明,直链淀粉含量和淀粉糊化特性受Wx-B1的影响最大,其次是Wx-D1,Wx-A1蛋白的影响最小.糯小麦的鉴定主要在细胞水平、蛋白质水平、分子水平上进行,I2-KI染色法和SDS-PAGE法是较常见的鉴定小麦糯性的方法.糯小麦野生型与突变型基因组成差异的发现使得分子标记技术在糯小麦的选育中得到了极大的发展和利用.  相似文献   

14.
氮钾配施对济南17淀粉理化特性的影响   总被引:7,自引:1,他引:7  
为给优质小麦的高产保质栽培提供借鉴,研究了氮钾配施对高产优质面包强筋小麦品种济南17籽粒淀粉理化特性的影响。结果表明,氮肥对济南17面粉直、支链淀粉和总淀粉含量均有一定的影响,氮肥水平提高,直、支链淀粉合成增加,淀粉总含量增加,但对淀粉化学特性的影响较小。后期施氮量提高,蛋白合成增加,直链淀粉合成能力相对减弱,但淀粉中直/支链淀粉比例相对稳定,峰值粘度没有明显变化。增施钾肥,面粉中直链淀粉含量下降,而支链淀粉和总淀粉含量显著上升,因此直/支链淀粉含量比例显著下降,但以中钾条件下支链淀粉和总淀粉含量最高。随着钾肥水平的提高,峰值粘度显著升高,但对膨胀势影响不显著。总之,氮肥处理对济南17面条品质的影响不大,但在合理的范围内增施钾肥可以显著改善淀粉理化特性,提高面条品质。  相似文献   

15.
Increases in the proportion of amylose in the starch of wheat grains result in higher levels of resistant starch, a fermentable dietary fiber associated with human health benefits. The objective of this study was to assess the effect of combined mutations in five STARCH BRANCHING ENZYME II (SBEII) genes on starch composition, grain yield and bread-making quality in two hexaploid wheat varieties. Significantly higher amylose (∼60%) and resistant starch content (10-fold) was detected in the SBEII mutants than in the wild-type controls. Mutant lines showed a significant decrease in total starch (6%), kernel weight (3%) and total grain yield (6%). Effects of the mutations in bread-making quality included increases in grain hardness, starch damage, water absorption and flour protein content; and reductions in flour extraction, farinograph development and stability times, starch viscosity, and loaf volume. Several traits showed significant interactions between genotypes, varieties, and environments, suggesting that some of the negative impacts of the combined SBEII mutations can be ameliorated by adequate selection of genetic background and growing location. The deployment of wheat varieties with increased resistant starch will likely require economic incentives to compensate growers and millers for the significant reductions detected in grain and flour yields.  相似文献   

16.
Reduced glutathione (GSH) commonly exists in wheat flour and has remarkable influence on gluten properties. In this study, effect of GSH on the gelatinization and retrogradation of wheat flour and wheat starch were investigated to better understand the GSH-gluten-starch interactions in wheat flour. Compared with wheat starch, wheat flour showed significant decreases in peak and final viscosity, and gelatinization onset temperature with increasing GSH concentration. GSH depolymerized gluten and thereby broke down the protein barrier around starch granules to make the starch easily gelatinized. However, the interaction between GSH and wheat starch restrained starch swelling. GSH addition resulted in weakened structure with higher water mobility in freshly gelatinized wheat flour dispersions but decreased water mobility in wheat starch dispersions. After storage at 4 °C for 7 d, GSH increased elasticity and retrogradation degree in wheat flour dispersions but retarded retrogradation in wheat starch dispersions. The results indicated that GSH promoted retrogradation of wheat flour, which mainly attributed to the depolymerized gluten embedding in the leached starch chains, and inhibiting the re-association of amylose, and subsequently promoted the starch intermolecular associations and starch retrogradation. This study could provide valuable information for the control of the quality of wheat flour-based products.  相似文献   

17.
糯性普通小麦的产生及其淀粉特性研究   总被引:46,自引:14,他引:46  
以江苏白火麦(缺失Wx-Dl)和关东107(缺失Wx-Al和Wx-Bl)为亲本配制杂交组合,采用花粉和籽粒剖面染色、蛋白质电泳进行筛选和鉴定,人工创造得到自然界不存在的糯性小麦,并对其直链淀粉含量及淀粉品质性状进行研究。结果表明,所得到的两个糯性小麦吕系直链淀粉含量很低(小于1%),其糊化特性、膨胀势特性亦与普通小麦品种不同;RVA高峰粘度明显低于其亲本,其高峰粘度出现较快,而且反弹值不明显;其淀粉膨胀能力远大于亲本。  相似文献   

18.
以缺失不同Wx蛋白小麦为试验材料,研究缺失不同Wx蛋白对小麦籽粒直链淀粉合成及淀粉特性的影响。试验结果表明,缺失不同Wx蛋白对小麦籽粒直链淀粉含量、积累量和积累速率、束缚态淀粉合成酶基因(GBSSI)基因相对表达量以及束缚态淀粉合成酶(GBSS)活性的影响顺序表现为:缺ABD型>缺AB型>缺B型>缺D型>缺A型>正常型;对淀粉最终粘度、反弹值以及糊化温度影响的表现顺序与其一致;对淀粉峰值粘度、低谷粘度、稀懈值以及膨胀势影响的表现顺序与此相反。相关分析表明,直链淀粉积累速率峰值、GBSSI相对表达量最大值以及GBSS活性峰值三者之间相互呈极显著正相关。说明GBSSI基因可能通过编码GBSS酶,从而控制小麦籽粒中直链淀粉的合成。  相似文献   

19.
Waxy maize (a standard starch of normal granule size) and five small granule starches from different botanical sources (rice, wheat B type, oat, quinoa and amaranth) were subjected to 2-octenyl-1-succinic anhydride (OSA) modification. Changes of pasting, gel texture, thermal and rheological properties were investigated. Different small granule starches showed quite different property changes after OSA modification. Pasting viscosity was generally increased in OSA starches, among which OSA oat starch had notably high peak and breakdown viscosity but low setback viscosity. Gel hardness of rice, wheat B type, oat and quinoa starches was reduced by OSA treatment, whereas that of waxy maize and amaranth starches was increased. Amylose content was considered to be the major factor influencing pasting, gel and thermal property of OSA starches. Esterification increased pseudoplastic flow behavior of all starches, while OSA oat starch uniquely had reduced flow consistency coefficient. The dynamic rheological properties were also changed differentially among OSA starches. Viscoelastic properties of rice, wheat B type, oat and quinoa starches were increased after OSA treatment, whereas those of waxy maize and amaranth starches were decreased. This study showed that diverse functionalities from OSA small granule starches may fulfil different demands in product development.  相似文献   

20.
小麦Wx蛋白及主要淀粉性状与面条品质的关系   总被引:2,自引:0,他引:2  
为了研究小麦Wx蛋白及淀粉主要性状与面条品质的关系,对26个小麦品种的Wx蛋白含量、直链淀粉含量、峰值粘度、膨胀势进行了测定,并探讨了其与面条品质的关系。结果表明.三种Wx蛋白亚基的含量与面条品质均呈显著负相关.相关系数分别为-0.598、-0.446和-0.451;直链淀粉含量与面条品质呈显著负相关,相关系数为-0.711;峰值粘度和膨胀势与面条品质呈显著正相关,相关系数分别为0.614和0.601。表明三种Wx蛋白亚基及直链淀粉含量、峰值粘度、膨胀势均与面条品质有密切关系,呈显著相关(正向或负向)。  相似文献   

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