共查询到20条相似文献，搜索用时 15 毫秒
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The glucocorticoid receptor protein binds to transfer RNA 总被引：6，自引：0，他引：6
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Molecular cloning of the chicken progesterone receptor 总被引：16，自引：0，他引：16
O M Conneely W P Sullivan D O Toft M Birnbaumer R G Cook B L Maxwell T Zarucki-Schulz G L Greene W T Schrader B W O'Malley 《Science (New York, N.Y.)》1986,233(4765):767-770
To define the functional domains of the progesterone receptor required for gene regulation, complementary DNA (cDNA) clones encoding the chicken progesterone receptor have been isolated from a chicken oviduct lambda gt11 cDNA expression library. Positive clones expressed antigenic determinants that cross-reacted with six monospecific antibodies derived from two independent sources. A 36-amino acid peptide sequence obtained by microsequencing of purified progesterone receptor was encoded by nucleotide sequences in the longest cDNA clone. Analysis of the amino acid sequence of the progesterone receptor deduced from the cDNA clones revealed a cysteine-rich region that was homologous to a region found in the estrogen and glucocorticoid receptors and to the avian erythroblastosis virus gag-erb-A fusion protein. Northern blot analysis with chicken progesterone receptor cDNA's indicated the existence of at least three messenger RNA species. These messages were found only in oviduct and could be induced by estrogens. 相似文献
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Creating a protein-based element of inheritance 总被引：1，自引：0，他引：1
Proteins capable of self-perpetuating changes in conformation and function (known as prions) can serve as genetic elements. To test whether novel prions could be created by recombinant methods, a yeast prion determinant was fused to the rat glucocorticoid receptor. The fusion protein existed in different heritable functional states, switched between states at a low spontaneous rate, and could be induced to switch by experimental manipulations. The complete change in phenotype achieved by transferring a prion determinant from one protein to another confirms the protein-only nature of prion inheritance and establishes a mechanism for engineering heritable changes in phenotype that should be broadly applicable. 相似文献
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Induced expression of the glucocorticoid receptor in the rat intermediate pituitary lobe 总被引：6，自引：0，他引：6
T Antakly A Sasaki A S Liotta M Palkovits D T Krieger 《Science (New York, N.Y.)》1985,229(4710):277-279
Synthesis and release of pro-opiomelanocortin-derived peptides are under differential regulation in the anterior and intermediate lobes of the pituitary. Glucocorticoids inhibit synthesis of pro-opiomelanocortin-related peptides in the anterior lobe but not in the intermediate lobe. These two lobes are also characterized by differences in neural innervation and blood flow, both of which may represent routes of access for regulatory factors (the intermediate lobe is avascular). Immunoreactive glucocorticoid receptor, which can be demonstrated in many tissues, is absent from the intermediate lobe. Immunocytochemistry was used to demonstrate the presence of immunoreactive glucocorticoid receptor in the intermediate lobe after pituitary stalk transection, neurointermediate lobe grafts to kidney capsule, or monolayer culture of neurointermediate pituitary cells. This appearance of the glucocorticoid receptor is presumably a consequence of removal of intermediate pituitary cells from neural influences that may be responsible for inhibiting their expression under normal conditions in vivo. 相似文献
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Human diabetes associated with a deletion of the tyrosine kinase domain of the insulin receptor 总被引：11，自引：0，他引：11
M Taira M Taira N Hashimoto F Shimada Y Suzuki A Kanatsuka F Nakamura Y Ebina M Tatibana H Makino 《Science (New York, N.Y.)》1989,245(4913):63-66
The insulin receptor has an intrinsic tyrosine kinase activity that is essential for signal transduction. A mutant insulin receptor gene lacking almost the entire kinase domain has been identified in an individual with type A insulin resistance and acanthosis nigricans. Insulin binding to the erythrocytes or cultured fibroblasts from this individual was normal. However receptor autophosphorylation and tyrosine kinase activity toward an exogenous substrate were reduced in partially purified insulin receptors from the proband's lymphocytes that had been transformed by Epstein-Barr virus. The insulin resistance associated with this mutated gene was inherited by the proband from her mother as an apparently autosomal dominant trait. Thus a deletion in one allele of the insulin receptor gene may be at least partly responsible for some instances of insulin-resistant diabetes. 相似文献
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Negative regulation by glucocorticoids through interference with a cAMP responsive enhancer 总被引：65，自引：0，他引：65
I E Akerblom E P Slater M Beato J D Baxter P L Mellon 《Science (New York, N.Y.)》1988,241(4863):350-353
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通过分离纯化卵泡抑制素受体,确定其受体类型,旨在为抑制素的作用机制研究奠定基础.采集30头长白猪垂体组织制备膜蛋白,用亲和层析法从该蛋白组分中分离、纯化出抑制素受体.SDS-PAGE结果发现该受体提取物中有6种蛋白,分子质量从14 ku到130 ku不等,表明抑制素受体可能不是特异性的单一受体,而是共受体.改良的EIASA结果证实,该受体提取物特异性活性为99 U/mg,能与抑制素结合,且与剂量呈正相关(R=0.993). 相似文献
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Glucocorticoid receptor-like antigen in lymphoma cell membranes: correlation to cell lysis 总被引：12，自引：0，他引：12
B Gametchu 《Science (New York, N.Y.)》1987,236(4800):456-461
S-49 mouse lymphoma cells undergo lysis when treated with glucocorticoids; the mechanism of this effect is not understood. A protein was detected in the plasma membrane of these cells by means of direct immunofluorescent labeling with a monoclonal antibody to the soluble glucocorticoid receptor. Cellular heterogeneity in the content of this glucocorticoid receptor-like molecule was evident. By immunoadsorption to antibody-coated tissue culture plates, the cells were separated into populations positive (100%) and depleted (38%) for this membrane antigen. Gel electrophoresis, specific immunoblot, and autoradiographic (binding of [3H]dexamethasone mesylate) analysis of the membrane proteins from the membrane antigen-positive group revealed multiple protein bands ranging in size from 85 to 145 kilodaltons. Furthermore, comparison of the glucocorticoid sensitivity of these groups of cells showed complete lysis of the membrane antigen-positive cells and only partial lysis of the antigen-deficient group, which suggests that the lysis response of cells to glucocorticoids is mediated by a glucocorticoid receptor-like molecule located in the plasma membrane. 相似文献
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己烯雌酚酶联免疫检测方法的建立 总被引：4，自引：0，他引：4
建立灵敏度高和特异性强的己烯雌酚残留检测分析方法。用人工合成的己烯雌酚免疫原多次免疫家兔,获取高效价抗体。纯化抗体经生物素标记,可同辣根过氧化物酶(HRP)标记的亲和素特异结合。采用纯化的抗体包被96孔板,形成固相抗体。加入不同浓度的己烯雌酚标准品后,再加入生物素标记抗体,以酶标记亲和素作为探针,经酶底物显色反应可获得良好的ELISA标准曲线。该方法检测灵敏度为0.125 ng/mL,可定量检测范围为0.25~15.60 ng/mL。该方法操作简便,具有较强灵敏度和特异性,可满足实践中一般样品的残留检测。 相似文献