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Takeshi Toyoda Jun-ichi Akagi Young-Man Cho Yasuko Mizuta Saeko Onami Isamu Suzuki Kumiko Ogawa 《Journal of toxicologic pathology》2013,26(2):215-221
To evaluate the potential role of DNA repair in bladder carcinogenesis, we performed an immunohistochemical analysis of expression of various DNA repair enzymes and γ-H2AX, a high-sensitivity marker of DNA double-strand breaks, in the urothelium of male F344 rats treated with N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN), a bladder-specific carcinogen. Our results clearly demonstrated that γ-H2AX aggregation was specifically generated in nuclei of bladder epithelial cells of BBN-treated rats, which was not found in untreated controls or mesenchymal cells. γ-H2AX-positive cells were detected not only in hyperplastic and neoplastic areas but also in the normal-like urothelium after BBN treatment. These data indicate that γ-H2AX has potential as a useful biomarker for early detection of genotoxicity in the rat urinary bladder. To the best of our knowledge, this is the first report demonstrating expression of γ-H2AX during bladder carcinogenesis. 相似文献
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Toshiaki SUMIYOSHI Tomomi TANAKA Hideo KAMOMAE 《The Journal of reproduction and development》2014,60(2):106-114
Lactating Holstein-Friesian cows kept in tie-stall barn were used as subjects in this
study. Rectal examination, ultrasonography and blood sampling were conducted every other
day and then daily after the day on which diameter of the corpus luteum decreased. After
the luteal diameter decreased for 2 consecutive days, rectal and ultrasound examinations,
blood sampling, and observation of estrous signs were conducted at 6-h intervals. Most of
the estrous signs became obvious with the increase in estradiol-17β (E2) and
became most remarkable 24 to 30 hours before ovulation, at which point the E2
peak and luteinizing hormone (LH) surge were achieved, and then weakened which progression
to ovulation. The correlation between the intensity of four estrous signs (hyperemia and
swelling of the intravaginal part of the uterus, opening of the external uterine orifice
and viscosity of the cervical mucus) and the plasma E2 concentration was higher
than that of three estrous signs (swelling of the vulva, contraction of the uterus,
diameter of uterine horn) and the plasma E2 concentration. The relaxation of
the intravaginal part of the uterus showed a unique change compared with the other estrous
signs, and it became most obvious 6, 12 and 18 h before ovulation; this obviously relaxed
period was consistent with the generally accepted theoretical optimal time for artificial
insemination (AI), i.e., 6 to 24 h after initiation of estrus. These results suggest that
observation of estrous signs by vaginoscopic examination gave useful information for
detection of the optimal timing of AI in the periovulatory period in lactating dairy cows
kept in a tie-stall barn. 相似文献
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Y. Brito‐Casillas P. Figueirinhas J.C. Wiebe L. Lpez‐Ríos D. Prez‐Barreto C. Melin A.M. Wgner 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(5):1405-1413
Background
Portable blood glucose meters (PBGMs) allow easy glucose measurements. As animal‐specific PBGMs are not available everywhere, those for humans are widely used.Objectives
To assess the accuracy and precision of 9 PBGMs in canine whole blood (WB) and plasma, based on the ISO 15197:2013.Animals
Fifty‐nine client‐owned dogs attending the Veterinary Teaching Hospital.Methods
Analytical evaluation of 100 blood samples was performed for accuracy and 23 for precision (glucose 29–579 mg/dL) following ISO recommendations. A PBGM was considered accurate if 95% of the measurements were within ±15 mg/dL from the reference when glucose was <100 mg/dL and within ±15% when it was ≥100 mg/dL, and if 99% of them were within zones A and B in error grid analysis (EG). A hexokinase‐based analyzer was used as reference. Ninety samples were assessed for hematocrit interferences.Results
Accuracy requirements were not fulfilled by any PBGM in WB (74% of measurements within the limits for the most accurate) and by 1 only in plasma. However, the EG analysis in WB was passed by 6 PBGM and by all in plasma. The most accurate were also the most precise, with coefficients of variation <5% in WB and <3% in plasma. Hematocrit correlated with bias against the reference method in 4 PBGM (r = −0.243 − [−0.371]; P < .021).Conclusions and Clinical Importance
This disparity among PBGM suggests that meters approved for humans need to be evaluated before use in other species. 相似文献12.
“洞·庭×碧·波”是湖南省蚕桑科学研究所承担国家“八·五”科技攻关课题和湖南省“八·五”科技重点攻关课题而育成的夏秋用双限性斑纹四元杂交新品种。洞和碧分别是限性品种“限1Ｂ”和“限2 Ａ” ,庭和波是限性品种“秋丰Ｂ”和“854Ｂ” ,它的配成和应用在国内同类研究中尚属首次。其一代杂交种表现出抗性强、好养、茧形大、高产稳产、丝质优的特点 ,特别是原蚕强健、雌雄鉴别容易、繁育系数高 ,是种场、蚕农、丝厂欢迎的新品种。目前 ,在湖南、湖北、重庆、四川、贵州、云南、广东、广西、江苏、浙江、河南、江西等省 (区 )市均有应… 相似文献
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湖·滨×明·光是我所承担湖南省“九·五”科技攻关课题育成的四元杂交桑蚕新品种，2003年3月获得湖南省农作物品种登记证书。该品种具有抗逆性强、好饲养、茧形大、高产稳产、丝多质优、一代杂交种繁育系数高、适应性广等特点，适宜在长江流域和南方蚕区及工业污染较为严重的地区推广应用。为加快科技成果转化，加速新品种的推广与应用，我所与湖南省蚕业开发中心共同承担了第六批国家茧丝绸风险基金项目“桑蚕新品种湖·滨×明·光良种的繁育与推广”。[第一段] 相似文献
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